Rw3G029390

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000710
Physical Location & Seq
Reverse (-)
45275102 .. 45275695
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G029390.1

Sequence Viewer

Length: 594 bp
ATGACAGTTGAAGCAATAATGAAAAATTTTGCTGATTTTAATACTAGTACTAGCGGAGCAAAAACTTCAAACATTTCAACCACAATTAAGTGGTCTGCTCCTCCTAGTTCTTTTGTTAAAATCAACTTTGATGGTTCAGTTTGTAGAGATTCCGCCGCTAGTGGTTTTGTTATTAGAGACCATGATGGAAGACCAATTATCGCTTCCACCAAGTATATTGGCAACTCTACTACCCCTGTCGCAGAAGCTACTGCTCTTAGGGATAGCCTGGTTGCGGTCAAAGACAAAGGTTTCACAAGAGTTGAAGTTGAAGGAGACTCGAAGCTGGTTATCGATGCAGTTAATGGAGGGGTGACTCCACCTTGGAGGCTGCTCAAATTAATTGAAGACATTAGAACTATTGCAACTTCCTTTTCTCAAATTTCCTTCAAACATATCTATAGGGAAGCAAACTTTGTAGCTGATGCCATTGCAAATTTAGGACATGGGGCTGCTTTTCCTATGTCTTGGATTGATAGGGTTCCTAGTGAGGCTTCTAGTGCCTTATTGTTTGACGTTGTAAACATTGGTTGCCCAAGAGGCTTCCTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

197

Amino Acids

21.22

Weight (kDa)

6.83

Isoelectric Point (pI)

27.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 42 - 160 3.9e-25 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 54, 153, 156, 275
AcsI RAATTY 3 cut(s) 25, 420, 475
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 274
AgsI TTSAA 7 cut(s) 11, 69, 78, 305, 311, 386, 430
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 1 cut(s) 267
AjuI GAANNNNNNNTTGG 2 cut(s) 187, 219
AluBI AGCT 3 cut(s) 248, 325, 461
AluI AGCT 3 cut(s) 248, 325, 461
Alw26I GTCTC 2 cut(s) 171, 309
ApeKI GCWGC 2 cut(s) 370, 491
ApoI RAATTY 3 cut(s) 25, 420, 475
AseI ATTAAT 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 196, 393
BbvI GCAGC 2 cut(s) 357, 478
BccI CCATC 2 cut(s) 125, 179
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 171, 309
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 6 cut(s) 45, 51, 105, 159, 525, 537
BfmI CTRYAG 1 cut(s) 439
BglI GCCNNNNNGGC 1 cut(s) 579
BisI GCNGC 3 cut(s) 156, 371, 492
BlsI GCNGC 3 cut(s) 157, 372, 493
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 2 cut(s) 325, 454
BpiI GAAGAC 2 cut(s) 196, 393
Bsa29I ATCGAT 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 362
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 1 cut(s) 269
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMI GCAATG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 2 cut(s) 357, 478
BshVI ATCGAT 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 274
BsmAI GTCTC 2 cut(s) 171, 309
Bso31I GGTCTC 1 cut(s) 171
BspACI CCGC 4 cut(s) 54, 153, 156, 275
BspDI ATCGAT 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 522
BspTNI GGTCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 468
BssECI CCNNGG 1 cut(s) 362
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 257
BstMAI GTCTC 2 cut(s) 171, 309
BstMWI GCNNNNNNNGC 2 cut(s) 539, 579
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 439
BstV1I GCAGC 2 cut(s) 357, 478
BstV2I GAAGAC 2 cut(s) 196, 393
Bsu15I ATCGAT 1 cut(s) 333
BsuTUI ATCGAT 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 1 cut(s) 48
CviAII CATG 2 cut(s) 182, 485
CviJI RGCY 8 cut(s) 248, 267, 325, 370, 461, 491, 533, 582
CviKI_1 RGCY 8 cut(s) 248, 267, 325, 370, 461, 491, 533, 582
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 257
EciI GGCGGA 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 2 cut(s) 185, 488
FaiI YATR 6 cut(s) 183, 216, 435, 441, 486, 503
FatI CATG 2 cut(s) 181, 484
Fnu4HI GCNGC 3 cut(s) 156, 371, 492
Fsp4HI GCNGC 3 cut(s) 156, 371, 492
FspBI CTAG 6 cut(s) 45, 51, 105, 159, 525, 537
GluI GCNGC 3 cut(s) 156, 371, 492
Hin1II CATG 2 cut(s) 185, 488
HinfI GANTC 3 cut(s) 149, 317, 355
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 562
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 2 cut(s) 305, 436
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 3 cut(s) 338, 404, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 579
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 2 cut(s) 185, 488
LmnI GCTCC 2 cut(s) 56, 103
LpnPI CCDG 4 cut(s) 249, 254, 281, 311
Lsp1109I GCAGC 2 cut(s) 357, 478
LweI GCATC 2 cut(s) 325, 454
MaeI CTAG 6 cut(s) 45, 51, 105, 159, 525, 537
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 352
MboII GAAGA 2 cut(s) 201, 398
MluCI AATT 7 cut(s) 25, 84, 195, 377, 381, 420, 475
MlyI GAGTC 2 cut(s) 311, 349
MnlI CCTC 5 cut(s) 111, 341, 360, 523, 572
MseI TTAA 6 cut(s) 39, 87, 117, 342, 380, 592
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 539, 579
NlaIII CATG 2 cut(s) 185, 488
NlaIV GGNNCC 1 cut(s) 522
NmuCI GTSAC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 3 cut(s) 157, 372, 493
PleI GAGTC 2 cut(s) 311, 349
PpsI GAGTC 2 cut(s) 311, 349
PshBI ATTAAT 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 522
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 6 cut(s) 39, 87, 117, 342, 380, 592
SatI GCNGC 3 cut(s) 156, 371, 492
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 2 cut(s) 311, 349
ScrFI CCNGG 1 cut(s) 269
SetI ASST 6 cut(s) 250, 292, 327, 364, 463, 558
SfaNI GCATC 2 cut(s) 325, 454
SfcI CTRYAG 1 cut(s) 439
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 7 cut(s) 25, 84, 195, 377, 381, 420, 475
SsiI CCGC 4 cut(s) 54, 153, 156, 275
SspMI CTAG 6 cut(s) 45, 51, 105, 159, 525, 537
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 320, 333
TasI AATT 7 cut(s) 25, 84, 195, 377, 381, 420, 475
TatI WGTACW 1 cut(s) 47
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 6 cut(s) 39, 87, 117, 342, 380, 592
Tru9I TTAA 6 cut(s) 39, 87, 117, 342, 380, 592
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 370, 491
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 35
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 3 cut(s) 25, 420, 475
XspI CTAG 6 cut(s) 45, 51, 105, 159, 525, 537
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.