Rorug05G0339800

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000710
Physical Location & Seq
Forward (+)
41312469 .. 41314541
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0339800.1

Sequence Viewer

Length: 1332 bp
ATGTCATCTTCAAATAATGACCATGATCAGGTTAAATCTGCAGGACTCTTCGAGAGGTTATGGGGGAAGATAGTTAAGTTTGGCTTGATGTTAAAAAAACTAGGGCAAGATGATCCAAGAAGAATTGTTCATTCTCTTAAAGTGGCACTTGCTGTTACATTAGTCTCCATGTTATTCTACTTTGAACCACTATATGACGGTTTTGGGCTTGCTGCTATGTGGGCTATTCTGACTGTTGTGATCGTTTTTGAATTTACTGTTGGAGCGACGATAGGACGAGGTTTGAATAGAATGATGGCAACTTTAGTAGCTGCTGCTCTTGGCATTGGAGTCCATCGATTAGCAACTCTTTCTGGAAAGCAAGGAGAGCCAATACTTATTGCTCTCTTTGTCTTCGTAGAAGCTGGAATAGTGACATTCTTCAGATTTATTCCCCAAATGAAGGCAAGATACGATTTTGGTCTGCTGGTATTCAACTTGACATTCTGCCTGATATCGGTGTCTGGCTATCGCGACGATGAGGTTATACGAATGGGATTCGAGAGAACAAATACCATCATCATTGGAAGCTGTACTGCTGTTGCTGTTTGTGTTTTCATTCGTCCAGTATGGATTGGCGTGGAACTTCACAATCAGATTGCTACCAACATGGAAAAGCTTGCCAACTTCTTAGAAGGATTTGGAGATGAATACTTTGTAGTATCTGAGAATGGCCAGACCAGAGACAAGTCATCTTCTCTTCAGGGATATAAAAGTGTTCTAACTTCAAAAAGCAGTGAAGACACCATGGCTAATTTGGCAAGATGGGAACCGGGCCATGGGAGGTTCAGGTTTCGTCATCCATGGAAACAATACTTGAAAGTTGGAACCCTAACTCGACAATGTGCGTTCAAAATTGAAGCTCTCAACAACTACCTTACCTCTGAAACCCAAACACCTCAGGACGTTAAAAGCATAATACAAGGGCCAAGCGTGGTTATCAGCTCAGAGTGTGGGAAAGCATTGAAGCAGTTAGCAACCTCAATGAGGAAAATGACCAAATCACCCTCAAACGATCCCCACATTGCAAAGTCTAAAGATGCCGCTGAAGAACTCAAAGCTGTTATAAGATCAAGCTTGTGTAAACACCAAGATGATTCCCTAAATATAATAAAAGATGGTGCAGTGGCATTACTACTCTTTGAAATTATCACATGTACAGAGAAAATTTCTGAGGCTGCGCATAAACTAGCATCCCAAGCACATTTTAAGGATGTAAAACCTAGAGTGACACCGGTCCAGGAAAACTCGGGGAATGATGGGCCGCACCATGTTATTACGATTGAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

443

Amino Acids

49.04

Weight (kDa)

8.92

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALMT PF11744 33 - 371 5.5e-137 Aluminium activated malate transporter
FUSC_2 PF13515 72 - 196 4.3e-08 Fusaric acid resistance protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
Acc16I TGCGCA 1 cut(s) 1221
AccII CGCG 1 cut(s) 513
AciI CCGC 2 cut(s) 1083, 1304
AclWI GGATC 2 cut(s) 107, 1049
AcoI YGGCCR 1 cut(s) 712
AcsI RAATTY 2 cut(s) 251, 1206
AcuI CTGAAG 3 cut(s) 406, 725, 1107
AfaI GTAC 2 cut(s) 574, 1198
AfiI CCNNNNNNNGG 5 cut(s) 28, 442, 496, 818, 1026
AflIII ACRYGT 1 cut(s) 1193
AgeI ACCGGT 1 cut(s) 1273
AjnI CCWGG 1 cut(s) 1278
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 8 cut(s) 311, 404, 570, 658, 902, 984, 1100, 1116
AluI AGCT 8 cut(s) 311, 404, 570, 658, 902, 984, 1100, 1116
Alw26I GTCTC 2 cut(s) 169, 717
AlwI GGATC 2 cut(s) 107, 1049
Ama87I CYCGRG 1 cut(s) 1288
AoxI GGCC 4 cut(s) 712, 814, 965, 1301
ApeKI GCWGC 4 cut(s) 212, 311, 314, 1217
ApoI RAATTY 2 cut(s) 251, 1206
AsiGI ACCGGT 1 cut(s) 1273
AspLEI GCGC 1 cut(s) 1222
AspS9I GGNCC 4 cut(s) 814, 965, 1276, 1301
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 1 cut(s) 1035
AvaI CYCGRG 1 cut(s) 1288
AvaII GGWCC 1 cut(s) 1276
AxyI CCTNAGG 1 cut(s) 939
BalI TGGCCA 1 cut(s) 714
BarI GAAGNNNNNNTAC 2 cut(s) 434, 466
BbsI GAAGAC 2 cut(s) 385, 786
BbvI GCAGC 4 cut(s) 199, 298, 301, 1204
BccI CCATC 6 cut(s) 289, 342, 563, 798, 1151, 1292
BciT130I CCWGG 1 cut(s) 1280
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 2 cut(s) 169, 717
BfaI CTAG 3 cut(s) 101, 1229, 1263
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 6 cut(s) 213, 312, 315, 1083, 1218, 1304
BlsI GCNGC 6 cut(s) 214, 313, 316, 1084, 1219, 1305
Bme1390I CCNGG 2 cut(s) 813, 1280
Bme18I GGWCC 1 cut(s) 1276
BmeT110I CYCGRG 1 cut(s) 1288
BmgT120I GGNCC 4 cut(s) 814, 965, 1276, 1301
BmiI GGNNCC 2 cut(s) 810, 868
BmrFI CCNGG 2 cut(s) 813, 1280
BmsI GCATC 2 cut(s) 1069, 1241
BoxI GACNNNNGTC 1 cut(s) 1274
BpiI GAAGAC 2 cut(s) 385, 786
BpuMI CCSGG 1 cut(s) 813
Bsa29I ATCGAT 1 cut(s) 337
BsaJI CCNNGG 3 cut(s) 786, 817, 842
BsaWI WCCGGW 1 cut(s) 1273
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 442, 496, 818, 1026
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 1 cut(s) 605
Bse21I CCTNAGG 1 cut(s) 939
Bse3DI GCAATG 1 cut(s) 1062
BseBI CCWGG 1 cut(s) 1280
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 786, 817, 842
BseGI GGATG 3 cut(s) 838, 1232, 1258
BseLI CCNNNNNNNGG 5 cut(s) 28, 442, 496, 818, 1026
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 4 cut(s) 696, 953, 999, 1203
BseNI ACTGG 1 cut(s) 605
BseXI GCAGC 4 cut(s) 199, 298, 301, 1204
BsgI GTGCAG 1 cut(s) 1182
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 4 cut(s) 714, 816, 967, 1303
BshTI ACCGGT 1 cut(s) 1273
BshVI ATCGAT 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 1288
BsiSI CCGG 2 cut(s) 812, 1274
BslI CCNNNNNNNGG 5 cut(s) 28, 442, 496, 818, 1026
BsmAI GTCTC 2 cut(s) 169, 717
BsnI GGCC 4 cut(s) 714, 816, 967, 1303
BsoBI CYCGRG 1 cut(s) 1288
Bsp1407I TGTACA 1 cut(s) 1196
Bsp143I GATC 5 cut(s) 25, 112, 240, 1054, 1109
Bsp19I CCATGG 3 cut(s) 786, 817, 842
Bsp68I TCGCGA 1 cut(s) 513
BspACI CCGC 2 cut(s) 1083, 1304
BspANI GGCC 4 cut(s) 714, 816, 967, 1303
BspCNI CTCAG 4 cut(s) 697, 952, 998, 1204
BspDI ATCGAT 1 cut(s) 337
BspFNI CGCG 1 cut(s) 513
BspLI GGNNCC 2 cut(s) 810, 868
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 2 cut(s) 107, 1049
BsrDI GCAATG 1 cut(s) 1062
BsrFI RCCGGY 1 cut(s) 1273
BsrGI TGTACA 1 cut(s) 1196
BsrI ACTGG 1 cut(s) 605
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 3 cut(s) 786, 817, 842
BssMI GATC 5 cut(s) 25, 112, 240, 1054, 1109
BssT1I CCWWGG 3 cut(s) 786, 817, 842
Bst2UI CCWGG 1 cut(s) 1280
Bst4CI ACNGT 3 cut(s) 200, 235, 259
Bst6I CTCTTC 2 cut(s) 53, 744
BstAUI TGTACA 1 cut(s) 1196
BstC8I GCNNGC 2 cut(s) 210, 660
BstDEI CTNAG 5 cut(s) 670, 705, 939, 985, 1212
BstDSI CCRYGG 3 cut(s) 786, 817, 842
BstENI CCTNNNNNAGG 1 cut(s) 1024
BstF5I GGATG 3 cut(s) 838, 1232, 1258
BstFNI CGCG 1 cut(s) 513
BstHHI GCGC 1 cut(s) 1222
BstKTI GATC 5 cut(s) 28, 115, 243, 1057, 1112
BstMAI GTCTC 2 cut(s) 169, 717
BstMBI GATC 5 cut(s) 25, 112, 240, 1054, 1109
BstMWI GCNNNNNNNGC 4 cut(s) 221, 367, 797, 1238
BstNI CCWGG 1 cut(s) 1280
BstNSI RCATGY 1 cut(s) 1197
BstPAI GACNNNNGTC 1 cut(s) 1274
BstSCI CCNGG 2 cut(s) 811, 1278
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 199, 298, 301, 1204
BstV2I GAAGAC 2 cut(s) 385, 786
Bsu15I ATCGAT 1 cut(s) 337
Bsu36I CCTNAGG 1 cut(s) 939
BsuRI GGCC 4 cut(s) 714, 816, 967, 1303
BsuTUI ATCGAT 1 cut(s) 337
BtgI CCRYGG 3 cut(s) 786, 817, 842
BtsCI GGATG 3 cut(s) 838, 1232, 1258
BtsI GCAGTG 2 cut(s) 781, 1170
BtsIMutI CAGTG 2 cut(s) 781, 1170
BtuMI TCGCGA 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 210, 660
CfoI GCGC 1 cut(s) 1222
Cfr10I RCCGGY 1 cut(s) 1273
Cfr13I GGNCC 4 cut(s) 814, 965, 1276, 1301
ClaI ATCGAT 1 cut(s) 337
Csp6I GTAC 2 cut(s) 573, 1197
CspAI ACCGGT 1 cut(s) 1273
CviAII CATG 8 cut(s) 23, 169, 649, 787, 818, 843, 1194, 1310
CviQI GTAC 2 cut(s) 573, 1197
DdeI CTNAG 5 cut(s) 670, 705, 939, 985, 1212
DpnI GATC 5 cut(s) 27, 114, 242, 1056, 1111
DpnII GATC 5 cut(s) 25, 112, 240, 1054, 1109
EaeI YGGCCR 1 cut(s) 712
Eam1104I CTCTTC 2 cut(s) 53, 744
EarI CTCTTC 2 cut(s) 53, 744
Eco130I CCWWGG 3 cut(s) 786, 817, 842
Eco32I GATATC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 1276
Eco57I CTGAAG 3 cut(s) 406, 725, 1107
Eco81I CCTNAGG 1 cut(s) 939
Eco88I CYCGRG 1 cut(s) 1288
EcoNI CCTNNNNNAGG 1 cut(s) 1024
EcoRII CCWGG 1 cut(s) 1278
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 3 cut(s) 786, 817, 842
ErhI CCWWGG 3 cut(s) 786, 817, 842
FaeI CATG 8 cut(s) 26, 172, 652, 790, 821, 846, 1197, 1313
FalI AAGNNNNNCTT 4 cut(s) 68, 100, 132, 164
FatI CATG 8 cut(s) 22, 168, 648, 786, 817, 842, 1193, 1309
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 6 cut(s) 213, 312, 315, 1083, 1218, 1304
FokI GGATG 3 cut(s) 825, 1219, 1265
Fsp4HI GCNGC 6 cut(s) 213, 312, 315, 1083, 1218, 1304
FspBI CTAG 3 cut(s) 101, 1229, 1263
FspI TGCGCA 1 cut(s) 1221
GlaI GCGC 1 cut(s) 1221
GluI GCNGC 6 cut(s) 213, 312, 315, 1083, 1218, 1304
HaeIII GGCC 4 cut(s) 714, 816, 967, 1303
HapII CCGG 2 cut(s) 812, 1274
HhaI GCGC 1 cut(s) 1222
Hin1II CATG 8 cut(s) 26, 172, 652, 790, 821, 846, 1197, 1313
Hin6I GCGC 1 cut(s) 1220
HinP1I GCGC 1 cut(s) 1220
HindIII AAGCTT 2 cut(s) 656, 1114
HinfI GANTC 4 cut(s) 45, 330, 537, 1136
HpaII CCGG 2 cut(s) 812, 1274
HphI GGTGA 1 cut(s) 1035
Hpy166II GTNNAC 1 cut(s) 1124
Hpy188I TCNGA 7 cut(s) 231, 425, 636, 706, 925, 988, 1213
Hpy188III TCNNGA 5 cut(s) 52, 354, 512, 541, 941
Hpy8I GTNNAC 1 cut(s) 1124
Hpy99I CGWCG 2 cut(s) 271, 518
HpyAV CCTTC 2 cut(s) 436, 668
HpyCH4III ACNGT 3 cut(s) 200, 235, 259
HpyCH4IV ACGT 1 cut(s) 945
HpyCH4V TGCA 3 cut(s) 41, 1067, 1163
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 367, 797, 1238
HpyF3I CTNAG 5 cut(s) 670, 705, 939, 985, 1212
HpySE526I ACGT 1 cut(s) 945
Hsp92II CATG 8 cut(s) 26, 172, 652, 790, 821, 846, 1197, 1313
HspAI GCGC 1 cut(s) 1220
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 5 cut(s) 25, 112, 240, 1054, 1109
LmnI GCTCC 1 cut(s) 263
Lsp1109I GCAGC 4 cut(s) 199, 298, 301, 1204
LweI GCATC 2 cut(s) 1069, 1241
MaeI CTAG 3 cut(s) 101, 1229, 1263
MaeII ACGT 1 cut(s) 945
MaeIII GTNAC 3 cut(s) 154, 412, 1267
MalI GATC 5 cut(s) 27, 114, 242, 1056, 1111
MboI GATC 5 cut(s) 25, 112, 240, 1054, 1109
MboII GAAGA 9 cut(s) 40, 79, 132, 385, 412, 726, 731, 791, 1100
MlsI TGGCCA 1 cut(s) 714
MluCI AATT 6 cut(s) 123, 251, 793, 894, 1185, 1206
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 2 cut(s) 39, 339
MmeI TCCRAC 2 cut(s) 241, 844
Mox20I TGGCCA 1 cut(s) 714
MscI TGGCCA 1 cut(s) 714
MseI TTAA 6 cut(s) 33, 75, 92, 138, 948, 1248
MslI CAYNNNNRTG 1 cut(s) 1131
Msp20I TGGCCA 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 1085
MspI CCGG 2 cut(s) 812, 1274
MspR9I CCNGG 2 cut(s) 813, 1280
MvaI CCWGG 1 cut(s) 1280
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 221, 367, 797, 1238
NciI CCSGG 1 cut(s) 813
NcoI CCATGG 3 cut(s) 786, 817, 842
NdeII GATC 5 cut(s) 25, 112, 240, 1054, 1109
NlaIII CATG 8 cut(s) 26, 172, 652, 790, 821, 846, 1197, 1313
NlaIV GGNNCC 2 cut(s) 810, 868
NmuCI GTSAC 2 cut(s) 412, 1267
NruI TCGCGA 1 cut(s) 513
NsbI TGCGCA 1 cut(s) 1221
NspI RCATGY 1 cut(s) 1197
PciI ACATGT 1 cut(s) 1193
PfeI GAWTC 2 cut(s) 537, 1136
PfoI TCCNGGA 1 cut(s) 1278
PinAI ACCGGT 1 cut(s) 1273
PkrI GCNGC 6 cut(s) 214, 313, 316, 1084, 1219, 1305
PleI GAGTC 2 cut(s) 39, 338
PpsI GAGTC 2 cut(s) 39, 338
PscI ACATGT 1 cut(s) 1193
PshAI GACNNNNGTC 1 cut(s) 1274
PsiI TTATAA 1 cut(s) 1106
Psp6I CCWGG 1 cut(s) 1278
PspGI CCWGG 1 cut(s) 1278
PspN4I GGNNCC 2 cut(s) 810, 868
PspPI GGNCC 4 cut(s) 814, 965, 1276, 1301
PstI CTGCAG 1 cut(s) 43
RruI TCGCGA 1 cut(s) 513
RsaI GTAC 2 cut(s) 574, 1198
RsaNI GTAC 2 cut(s) 573, 1197
RseI CAYNNNNRTG 1 cut(s) 1131
SaqAI TTAA 6 cut(s) 33, 75, 92, 138, 948, 1248
SatI GCNGC 6 cut(s) 213, 312, 315, 1083, 1218, 1304
Sau3AI GATC 5 cut(s) 25, 112, 240, 1054, 1109
Sau96I GGNCC 4 cut(s) 814, 965, 1276, 1301
SchI GAGTC 2 cut(s) 39, 339
ScrFI CCNGG 2 cut(s) 813, 1280
SfaNI GCATC 2 cut(s) 1069, 1241
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 1276
SmiMI CAYNNNNRTG 1 cut(s) 1131
Sse9I AATT 6 cut(s) 123, 251, 793, 894, 1185, 1206
SsiI CCGC 2 cut(s) 1083, 1304
SspMI CTAG 3 cut(s) 101, 1229, 1263
StyD4I CCNGG 2 cut(s) 811, 1278
StyI CCWWGG 3 cut(s) 786, 817, 842
TaaI ACNGT 3 cut(s) 200, 235, 259
TaiI ACGT 1 cut(s) 948
TaqI TCGA 4 cut(s) 51, 337, 540, 877
TasI AATT 6 cut(s) 123, 251, 793, 894, 1185, 1206
TatI WGTACW 2 cut(s) 572, 1196
TauI GCSGC 2 cut(s) 1085, 1306
TfiI GAWTC 2 cut(s) 537, 1136
Tru1I TTAA 6 cut(s) 33, 75, 92, 138, 948, 1248
Tru9I TTAA 6 cut(s) 33, 75, 92, 138, 948, 1248
TscAI CASTG 2 cut(s) 781, 1170
TseFI GTSAC 2 cut(s) 412, 1267
TseI GCWGC 4 cut(s) 212, 311, 314, 1217
Tsp45I GTSAC 2 cut(s) 412, 1267
TspDTI ATGAA 4 cut(s) 119, 455, 586, 702
TspRI CASTG 2 cut(s) 781, 1170
VpaK11BI GGWCC 1 cut(s) 1276
XagI CCTNNNNNAGG 1 cut(s) 1024
XapI RAATTY 2 cut(s) 251, 1206
XceI RCATGY 1 cut(s) 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 793
XspI CTAG 3 cut(s) 101, 1229, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.