FvH4_4g02991

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000710
Physical Location & Seq
Reverse (-)
2647238 .. 2647699
462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02991.t1

Sequence Viewer

Length: 336 bp
ATGAATGCATGTGAGTGGCTATTTGCTTTTTTAGCTTTTTTGGCAAGTGTAGAAGGTGACTCGAAGCTGGTGATTGATGTCATCAATGGGAAATTATCCCCTCCTTGGAGATTGCAGAAGATTGTCCAAGATATTCGGATTATTGCTATCTCTTTCTCCTCAGTTTGTTTTAACCATGTATACAGAGAAGCAAACTTTGTGGCGGATGCTTTTGCAAATGAAGGCCACCAACTCCCGAATGGTTGTGAGTGGCAAGATGGATACCCAGCAGCTGTTGCTAAAGCGCTGATATTTGATACTGTTAACTCGGGTTGTCCGAGAGGTTTGTCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

112

Amino Acids

12.22

Weight (kDa)

5.16

Isoelectric Point (pI)

20.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 17 - 74 5.9e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 180
AciI CCGC 1 cut(s) 203
AfeI AGCGCT 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 105
AluBI AGCT 3 cut(s) 35, 67, 272
AluI AGCT 3 cut(s) 35, 67, 272
AlwNI CAGNNNCTG 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 307
Aor51HI AGCGCT 1 cut(s) 285
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 269
AspLEI GCGC 1 cut(s) 286
AsuHPI GGTGA 2 cut(s) 68, 82
AvaI CYCGRG 1 cut(s) 307
BbvI GCAGC 1 cut(s) 281
BccI CCATC 1 cut(s) 251
BciVI GTATCC 1 cut(s) 254
BfoI RGCGCY 1 cut(s) 287
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 307
BmsI GCATC 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 104
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 1 cut(s) 281
BseYI CCCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 307
BslI CCNNNNNNNGG 1 cut(s) 105
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 307
BspACI CCGC 1 cut(s) 203
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 104
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 104
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 301
BstAPI GCANNNNNTGC 1 cut(s) 275
BstDEI CTNAG 1 cut(s) 160
BstF5I GGATG 1 cut(s) 211
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 275
BstNSI RCATGY 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 281
BstZ17I GTATAC 1 cut(s) 181
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 1 cut(s) 211
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 1 cut(s) 286
CspCI CAANNNNNGTGG 2 cut(s) 180, 215
CviAII CATG 2 cut(s) 9, 176
CviJI RGCY 5 cut(s) 19, 35, 67, 225, 272
CviKI_1 RGCY 5 cut(s) 19, 35, 67, 225, 272
DdeI CTNAG 1 cut(s) 160
EciI GGCGGA 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 104
Eco47III AGCGCT 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 104
EcoT22I ATGCAT 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 2 cut(s) 12, 179
FaiI YATR 3 cut(s) 10, 177, 181
FatI CATG 2 cut(s) 8, 175
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 1 cut(s) 270
GlaI GCGC 1 cut(s) 285
GluI GCNGC 1 cut(s) 270
GsaI CCCAGC 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 1 cut(s) 225
HhaI GCGC 1 cut(s) 286
Hin1II CATG 2 cut(s) 12, 179
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 1 cut(s) 59
HpaI GTTAAC 1 cut(s) 304
HphI GGTGA 2 cut(s) 68, 82
Hpy166II GTNNAC 2 cut(s) 181, 304
Hpy188I TCNGA 2 cut(s) 138, 318
Hpy188III TCNNGA 1 cut(s) 235
Hpy8I GTNNAC 2 cut(s) 181, 304
HpyAV CCTTC 2 cut(s) 47, 215
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 3 cut(s) 8, 115, 215
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 275
HpyF3I CTNAG 1 cut(s) 160
Hsp92II CATG 2 cut(s) 12, 179
HspAI GCGC 1 cut(s) 284
KspAI GTTAAC 1 cut(s) 304
LpnPI CCDG 2 cut(s) 53, 279
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 1 cut(s) 196
MaeIII GTNAC 1 cut(s) 56
MboII GAAGA 1 cut(s) 130
MluCI AATT 1 cut(s) 92
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 3 cut(s) 111, 169, 314
Mph1103I ATGCAT 1 cut(s) 10
MseI TTAA 2 cut(s) 171, 303
MslI CAYNNNNRTG 1 cut(s) 13
MspA1I CMGCKG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 10
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 275
NlaIII CATG 2 cut(s) 12, 179
NmuCI GTSAC 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 10
NspI RCATGY 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 314
PctI GAATGC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 271
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 272
RseI CAYNNNNRTG 1 cut(s) 13
SaqAI TTAA 2 cut(s) 171, 303
SatI GCNGC 1 cut(s) 270
SchI GAGTC 1 cut(s) 53
SetI ASST 5 cut(s) 37, 58, 69, 274, 325
SfaNI GCATC 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 13
Sse9I AATT 1 cut(s) 92
SsiI CCGC 1 cut(s) 203
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 301
TaqI TCGA 1 cut(s) 62
TasI AATT 1 cut(s) 92
Tru1I TTAA 2 cut(s) 171, 303
Tru9I TTAA 2 cut(s) 171, 303
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 17, 234
XceI RCATGY 1 cut(s) 12
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.