Rw2G025350

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000710
Physical Location & Seq
Forward (+)
37850035 .. 37850640
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025350.1

Sequence Viewer

Length: 606 bp
ATGTCAATTGTTATGTCAGTTGAAGCAATAATGAAAAGCTTTATTGATTTTAACACTAGTACTAGTGGAGCAAAAACTTCAAACCTTTCAACCACAATTAAGTGGTCTGCTCCTCCTAGTTCTTTTGTTAAAATCAATTTTGATGGCTCAGTTAGAAGAGATTCAGCTACTAGTGGTTTCGTTATCAGAGACCATAATGGAAGACCAGTTATTGTTGCAACCAAGTGTGTTGGCAATTCTTCTACCCATGTTGCAAAAGCTACTGCGCTCAGGGATAGTCTAATTGCGGCCAAAGACAAAGGTTTTACAAGAGTTGAAGTTGAAGGAGACTCAAAGCTGGTTATTGATGCAGTTACTGGAATGATGATTCCACCGTGGAGGCTTCTCAAATTGATTGAAGACATTAGAACCATTGCAGCTTCCTTCTCTCAAATTACCTTTAAGCATATCTATAGGGAGGCAAACTTTGTAGCTGATGCTATTGCAAATTTAGGACATGATGCTGCTTCTCCTATGTCGTGGAGTGATAGGGTCCCAAGTGAGGCTTCTAGAGCCTTAATTTTTGATGTTGTAAACTTTGGTTGCCCAAGAGGCTTCATTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

201

Amino Acids

21.8

Weight (kDa)

9.23

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 46 - 164 2.7e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 487
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 541
AgsI TTSAA 6 cut(s) 23, 81, 90, 317, 323, 398
AhlI ACTAGT 3 cut(s) 56, 62, 170
AjuI GAANNNNNNNTTGG 2 cut(s) 529, 561
AluBI AGCT 6 cut(s) 39, 167, 260, 337, 419, 473
AluI AGCT 6 cut(s) 39, 167, 260, 337, 419, 473
Alw26I GTCTC 2 cut(s) 183, 321
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 2 cut(s) 416, 503
ApoI RAATTY 1 cut(s) 487
Asp700I GAANNNNTTC 1 cut(s) 160
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 532
AvaII GGWCC 1 cut(s) 532
BbsI GAAGAC 2 cut(s) 208, 405
BbvI GCAGC 2 cut(s) 428, 490
BccI CCATC 1 cut(s) 137
BcoDI GTCTC 2 cut(s) 183, 321
BcuI ACTAGT 3 cut(s) 56, 62, 170
BfaI CTAG 5 cut(s) 57, 63, 117, 171, 549
BfmI CTRYAG 1 cut(s) 451
BglI GCCNNNNNGGC 1 cut(s) 591
BisI GCNGC 3 cut(s) 288, 417, 504
BlsI GCNGC 3 cut(s) 289, 418, 505
BmcAI AGTACT 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 2 cut(s) 533, 534
BmsI GCATC 3 cut(s) 337, 466, 490
BpiI GAAGAC 2 cut(s) 208, 405
Bpu10I CCTNAGC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 374
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 2 cut(s) 206, 361
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 374
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 162, 283
BseNI ACTGG 2 cut(s) 206, 361
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 428, 490
BshFI GGCC 1 cut(s) 290
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 2 cut(s) 183, 321
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 1 cut(s) 183
BspACI CCGC 1 cut(s) 287
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 2 cut(s) 161, 282
BspLI GGNNCC 2 cut(s) 533, 534
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 2 cut(s) 206, 361
BssECI CCNNGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 1 cut(s) 151
BstDEI CTNAG 2 cut(s) 148, 269
BstDSI CCRYGG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 268
BstMAI GTCTC 2 cut(s) 183, 321
BstMWI GCNNNNNNNGC 2 cut(s) 551, 591
BstSFI CTRYAG 1 cut(s) 451
BstV1I GCAGC 2 cut(s) 428, 490
BstV2I GAAGAC 2 cut(s) 208, 405
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 1 cut(s) 374
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 60
CviAII CATG 2 cut(s) 248, 497
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 148, 269
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 532
EcoO109I RGGNCCY 1 cut(s) 532
FaeI CATG 2 cut(s) 251, 500
FaiI YATR 7 cut(s) 14, 195, 249, 447, 453, 498, 515
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 247, 496
Fnu4HI GCNGC 3 cut(s) 288, 417, 504
Fsp4HI GCNGC 3 cut(s) 288, 417, 504
FspBI CTAG 5 cut(s) 57, 63, 117, 171, 549
GlaI GCGC 1 cut(s) 267
GluI GCNGC 3 cut(s) 288, 417, 504
HaeIII GGCC 1 cut(s) 290
HhaI GCGC 1 cut(s) 268
Hin1II CATG 2 cut(s) 251, 500
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 161, 329, 367
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 1 cut(s) 549
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 2 cut(s) 317, 433
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 218, 254, 350, 416, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 551, 591
HpyF3I CTNAG 2 cut(s) 148, 269
Hsp92II CATG 2 cut(s) 251, 500
HspAI GCGC 1 cut(s) 266
KflI GGGWCCC 1 cut(s) 532
LmnI GCTCC 2 cut(s) 68, 115
LpnPI CCDG 4 cut(s) 219, 256, 323, 342
Lsp1109I GCAGC 2 cut(s) 428, 490
LweI GCATC 3 cut(s) 337, 466, 490
MaeI CTAG 5 cut(s) 57, 63, 117, 171, 549
MaeIII GTNAC 1 cut(s) 352
MboII GAAGA 4 cut(s) 168, 213, 231, 410
MfeI CAATTG 1 cut(s) 6
MluCI AATT 9 cut(s) 6, 96, 136, 235, 282, 389, 432, 487, 558
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 5 cut(s) 123, 372, 451, 535, 584
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
MunI CAATTG 1 cut(s) 6
MwoI GCNNNNNNNGC 2 cut(s) 551, 591
NlaIII CATG 2 cut(s) 251, 500
NlaIV GGNNCC 2 cut(s) 533, 534
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 2 cut(s) 161, 367
PkrI GCNGC 3 cut(s) 289, 418, 505
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 533, 534
PspPI GGNCC 1 cut(s) 532
PspPPI RGGWCCY 1 cut(s) 532
PstNI CAGNNNCTG 1 cut(s) 356
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SaqAI TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
SatI GCNGC 3 cut(s) 288, 417, 504
Sau96I GGNCC 1 cut(s) 532
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 1 cut(s) 323
SetI ASST 9 cut(s) 41, 87, 169, 262, 304, 339, 421, 440, 475
SfaNI GCATC 3 cut(s) 337, 466, 490
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 532
SpeI ACTAGT 3 cut(s) 56, 62, 170
Sse9I AATT 9 cut(s) 6, 96, 136, 235, 282, 389, 432, 487, 558
SsiI CCGC 1 cut(s) 287
SspMI CTAG 5 cut(s) 57, 63, 117, 171, 549
TaaI ACNGT 1 cut(s) 375
TasI AATT 9 cut(s) 6, 96, 136, 235, 282, 389, 432, 487, 558
TatI WGTACW 1 cut(s) 59
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 2 cut(s) 161, 367
Tru1I TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
Tru9I TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
TseI GCWGC 2 cut(s) 416, 503
TspDTI ATGAA 2 cut(s) 47, 586
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 1 cut(s) 487
XbaI TCTAGA 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 5 cut(s) 57, 63, 117, 171, 549
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.