Rw5G023470

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000710
Physical Location & Seq
Forward (+)
33080013 .. 33080618
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023470.1

Sequence Viewer

Length: 606 bp
ATGTCAATTGTTATATCAGTTGAAGCAATAATGAAAAGCTTTGCAGATCTTAACACTAGTACTAGTGGAGAAAAAACTTCAAACCTTTCAACCACAATTAAGTGGTCTGCTCCTCCTAGTTCTTTTGTTAAAATCAATTTTGATGGCTCAGTTAGAAGAGATTCAGCTGCTACTAGTTTCGTTATCAGAGACCATAATGGAAGACCAGTTATTGCTGCAACCAAGTGTGTTGGCAATTCTTCTACTCCTGTTGCAGAAGCTACTGCACTCAGGGATAGTCTAATTGCAGCCAAAGACAAAGGTTTTACAAGAGTTGAAGTTGAAGGAGACTCAAAGCTGGTTATTGATGCAGTTACAGGAAGGGTGATTCCACCGTGGAGGCTACTCAAATTGATTGAAGACATTAGAACCATTGCAACTTCATTTTCTCAAATTACCTTTAAGCATATCTATAGGGAAGCAAACTTTGTAGCTGATGCTATTGCAAATTTAGGACATAATGCTGCTTCTCCTATGTCTTGGAGTGATAGGGTCCCAAGTGAGGCTTCTAGAGCCTTAATTTTTGACGTTGTAAACTTTGGTTGCCCAAGAGACTTCCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

201

Amino Acids

21.79

Weight (kDa)

8.62

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 46 - 164 4.2e-25 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 487
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 541
AgsI TTSAA 6 cut(s) 23, 81, 90, 317, 323, 398
AhlI ACTAGT 3 cut(s) 56, 62, 173
AjuI GAANNNNNNNTTGG 2 cut(s) 529, 561
AluBI AGCT 5 cut(s) 39, 167, 260, 337, 473
AluI AGCT 5 cut(s) 39, 167, 260, 337, 473
Alw26I GTCTC 3 cut(s) 183, 321, 585
ApeKI GCWGC 4 cut(s) 167, 215, 287, 503
ApoI RAATTY 1 cut(s) 487
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 532
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 532
BbsI GAAGAC 2 cut(s) 208, 405
BbvI GCAGC 4 cut(s) 154, 202, 299, 490
BccI CCATC 1 cut(s) 137
BcoDI GTCTC 3 cut(s) 183, 321, 585
BcuI ACTAGT 3 cut(s) 56, 62, 173
BfaI CTAG 5 cut(s) 57, 63, 117, 174, 549
BfmI CTRYAG 1 cut(s) 451
BglII AGATCT 1 cut(s) 46
BisI GCNGC 4 cut(s) 168, 216, 288, 504
BlsI GCNGC 4 cut(s) 169, 217, 289, 505
BmcAI AGTACT 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 2 cut(s) 533, 534
BmsI GCATC 2 cut(s) 337, 466
BpiI GAAGAC 2 cut(s) 208, 405
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 374
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 374
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 162, 283
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 4 cut(s) 154, 202, 299, 490
BsgI GTGCAG 1 cut(s) 249
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 3 cut(s) 183, 321, 585
BsmFI GGGAC 1 cut(s) 518
Bso31I GGTCTC 1 cut(s) 183
Bsp143I GATC 1 cut(s) 46
BspCNI CTCAG 2 cut(s) 161, 282
BspLI GGNNCC 2 cut(s) 533, 534
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 1 cut(s) 151
BstDEI CTNAG 2 cut(s) 148, 269
BstDSI CCRYGG 1 cut(s) 374
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 3 cut(s) 183, 321, 585
BstMBI GATC 1 cut(s) 46
BstMWI GCNNNNNNNGC 1 cut(s) 551
BstSFI CTRYAG 1 cut(s) 451
BstV1I GCAGC 4 cut(s) 154, 202, 299, 490
BstV2I GAAGAC 2 cut(s) 208, 405
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BtgI CCRYGG 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 60
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 148, 269
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 532
EcoO109I RGGNCCY 1 cut(s) 532
FaiI YATR 6 cut(s) 14, 195, 447, 453, 498, 515
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 1 cut(s) 518
Fnu4HI GCNGC 4 cut(s) 168, 216, 288, 504
Fsp4HI GCNGC 4 cut(s) 168, 216, 288, 504
FspBI CTAG 5 cut(s) 57, 63, 117, 174, 549
GluI GCNGC 4 cut(s) 168, 216, 288, 504
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 161, 329, 367
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 1 cut(s) 549
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 2 cut(s) 317, 354
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 8 cut(s) 44, 218, 254, 266, 287, 350, 416, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 551
HpyF3I CTNAG 2 cut(s) 148, 269
HpySE526I ACGT 1 cut(s) 567
KflI GGGWCCC 1 cut(s) 532
Kzo9I GATC 1 cut(s) 46
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 5 cut(s) 219, 256, 261, 323, 342
Lsp1109I GCAGC 4 cut(s) 154, 202, 299, 490
LweI GCATC 2 cut(s) 337, 466
MaeI CTAG 5 cut(s) 57, 63, 117, 174, 549
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 1 cut(s) 352
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 4 cut(s) 168, 213, 231, 410
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 1 cut(s) 46
MluCI AATT 9 cut(s) 6, 96, 136, 235, 282, 389, 432, 487, 558
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 3 cut(s) 123, 372, 535
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
MspA1I CMGCKG 1 cut(s) 167
MunI CAATTG 1 cut(s) 6
MwoI GCNNNNNNNGC 1 cut(s) 551
NdeII GATC 1 cut(s) 46
NlaIV GGNNCC 2 cut(s) 533, 534
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 2 cut(s) 161, 367
PkrI GCNGC 4 cut(s) 169, 217, 289, 505
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 533, 534
PspPI GGNCC 1 cut(s) 532
PspPPI RGGWCCY 1 cut(s) 532
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SaqAI TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
SatI GCNGC 4 cut(s) 168, 216, 288, 504
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 1 cut(s) 532
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 1 cut(s) 323
SetI ASST 9 cut(s) 41, 87, 169, 262, 304, 339, 440, 475, 570
SfaNI GCATC 2 cut(s) 337, 466
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 532
SpeI ACTAGT 3 cut(s) 56, 62, 173
Sse9I AATT 9 cut(s) 6, 96, 136, 235, 282, 389, 432, 487, 558
SspMI CTAG 5 cut(s) 57, 63, 117, 174, 549
TaaI ACNGT 1 cut(s) 375
TaiI ACGT 1 cut(s) 570
TasI AATT 9 cut(s) 6, 96, 136, 235, 282, 389, 432, 487, 558
TatI WGTACW 1 cut(s) 59
TfiI GAWTC 2 cut(s) 161, 367
Tru1I TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
Tru9I TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
TseI GCWGC 4 cut(s) 167, 215, 287, 503
TspDTI ATGAA 2 cut(s) 47, 411
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 1 cut(s) 487
XbaI TCTAGA 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 5 cut(s) 57, 63, 117, 174, 549
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.