Rroxscaffold_7G00193750

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000710
Physical Location & Seq
Reverse (-)
35298370 .. 35299347
978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193750.1

Sequence Viewer

Length: 288 bp
ATGGTAGCAGCTTCAGCTACTGTACTAGCTAACATTGGAACTTCAAATAGGCAAGCAGGGAGACATTCTATTCAAAACCAGCCATCCACTATAAAGTGGATCTCTCCTCCTGCTTGCAGTGCTAAATTAAACTTTGATAACACTGTAAATTGGTCTTCTGATGTTGGAGGTTTTGTTATCCGTGACAGCCATGGCAACCCCCTTGTAGCAGCTACTAGAAATGCCGGATCCACCACAGTATTTTTGGCAGAAGCTAATGCACTAAGAGATGGACTCTTATGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

95

Amino Acids

9.89

Weight (kDa)

8.89

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 107, 222, 235
AfaI GTAC 1 cut(s) 24
AgsI TTSAA 2 cut(s) 45, 74
AluBI AGCT 5 cut(s) 11, 17, 29, 212, 254
AluI AGCT 5 cut(s) 11, 17, 29, 212, 254
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 3 cut(s) 107, 222, 235
AlwNI CAGNNNCTG 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 8, 209
BamHI GGATCC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 20, 221
BccI CCATC 2 cut(s) 91, 263
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 2 cut(s) 26, 216
BisI GCNGC 2 cut(s) 9, 210
BlsI GCNGC 2 cut(s) 10, 211
BmiI GGNNCC 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 147
BplI GAGNNNNNCTC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 2 cut(s) 20, 221
BsiSI CCGG 1 cut(s) 225
BsmAI GTCTC 1 cut(s) 55
Bsp143I GATC 2 cut(s) 99, 227
Bsp19I CCATGG 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 229
BspPI GGATC 3 cut(s) 107, 222, 235
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 2 cut(s) 99, 227
BssT1I CCWWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 22, 145, 238
BstC8I GCNNGC 2 cut(s) 54, 115
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 83
BstKTI GATC 2 cut(s) 102, 230
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 2 cut(s) 99, 227
BstMWI GCNNNNNNNGC 2 cut(s) 14, 119
BstV1I GCAGC 2 cut(s) 20, 221
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 99, 227
BstYI RGATCY 2 cut(s) 99, 227
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 1 cut(s) 83
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 2 cut(s) 124, 141
Cac8I GCNNGC 2 cut(s) 54, 115
CaiI CAGNNNCTG 1 cut(s) 20
Csp6I GTAC 1 cut(s) 23
CviAII CATG 2 cut(s) 191, 285
CviJI RGCY 7 cut(s) 11, 17, 29, 82, 189, 212, 254
CviKI_1 RGCY 7 cut(s) 11, 17, 29, 82, 189, 212, 254
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 1 cut(s) 263
DpnI GATC 2 cut(s) 101, 229
DpnII GATC 2 cut(s) 99, 227
Eco130I CCWWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 2 cut(s) 194, 288
FaiI YATR 4 cut(s) 92, 192, 280, 286
FatI CATG 2 cut(s) 190, 284
Fnu4HI GCNGC 2 cut(s) 9, 210
FokI GGATG 1 cut(s) 70
Fsp4HI GCNGC 2 cut(s) 9, 210
FspBI CTAG 2 cut(s) 26, 216
GluI GCNGC 2 cut(s) 9, 210
HapII CCGG 1 cut(s) 225
Hin1II CATG 2 cut(s) 194, 288
HinfI GANTC 1 cut(s) 273
HpaII CCGG 1 cut(s) 225
Hpy188I TCNGA 1 cut(s) 160
HpyCH4III ACNGT 3 cut(s) 22, 145, 238
HpyCH4V TGCA 3 cut(s) 117, 260, 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 119
HpyF3I CTNAG 1 cut(s) 263
Hsp92II CATG 2 cut(s) 194, 288
Kzo9I GATC 2 cut(s) 99, 227
LpnPI CCDG 4 cut(s) 42, 92, 123, 238
Lsp1109I GCAGC 2 cut(s) 20, 221
MaeI CTAG 2 cut(s) 26, 216
MaeIII GTNAC 1 cut(s) 182
MalI GATC 2 cut(s) 101, 229
MboI GATC 2 cut(s) 99, 227
MboII GAAGA 1 cut(s) 147
MflI RGATCY 2 cut(s) 99, 227
MluCI AATT 2 cut(s) 125, 148
MlyI GAGTC 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 2 cut(s) 117, 161
MseI TTAA 1 cut(s) 128
MspI CCGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 14, 119
NcoI CCATGG 1 cut(s) 190
NdeII GATC 2 cut(s) 99, 227
NlaIII CATG 2 cut(s) 194, 288
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 182
PkrI GCNGC 2 cut(s) 10, 211
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 229
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 2 cut(s) 99, 227
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 9, 210
Sau3AI GATC 2 cut(s) 99, 227
SchI GAGTC 1 cut(s) 267
SetI ASST 6 cut(s) 13, 19, 31, 172, 214, 256
Sse9I AATT 2 cut(s) 125, 148
SspMI CTAG 2 cut(s) 26, 216
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 3 cut(s) 22, 145, 238
TasI AATT 2 cut(s) 125, 148
TatI WGTACW 1 cut(s) 22
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 2 cut(s) 124, 148
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 2 cut(s) 8, 209
Tsp45I GTSAC 1 cut(s) 182
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 124, 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XspI CTAG 2 cut(s) 26, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.