Rw5G021940

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014323
Physical Location & Seq
Reverse (-)
29867494 .. 29869327
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021940.1

Sequence Viewer

Length: 558 bp
ATGGCCTCAAAGCTGTTGTTGATCGCAATCGTCATCGTTAACATCATCGCTTTTGGGCTCGCTGTTGCAGCTGAGCAGAGGAGAAGCCATGCAAATATCGTGCAAGATAATGAAAAGAACTACAACTATTGTGTCTATGACTCAGACATTGCAACAGGCTACGGTGTTGGAGCATTTTTGTTCCTCTTGGTATCTCAGGTTATCGTAATGTTGGTAAGCCGATGCTTTTGTTGCGGAAAGCCATTGAGCCCTGGAGGAGCAAGAGCCTGGGCAGTCGTCCTTTTCATAACCTGCTGGATCTTTTTCTTCATTGCTGAGGTGTGCTTGCTGGCGGGTTCCGTGAGTAACGCCTACCACACCAAGTACAGGACCATTTTCGGCGAAGACCCTCCAGATTGCCAGACATTGAGAAAGGGAGTTTTTGGGGCAGGAGCAGCTTTCATTTTCCTGAACACAATTGTGTCCTCGTTCTACTACACTAACTATTCCAGAGCCAGGGCAAGCTTTCAGTCATACGGAACTGGAGAGGCCGCTGTTGGTCTCGGCACCTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

185

Amino Acids

20.16

Weight (kDa)

8.68

Isoelectric Point (pI)

26.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 152 2.4e-32 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27435
fragaria_vesca FvH4_3g20620
malus_domestica MD03G1223600.v1.1 MD11G1241500.v1.1
prunus_persica Prupe.4G185900_v2.0.a1
pyrus_communis pycom03g17190 pycom11g21310
rosa_chinensis RchiOBHm_Chr5g0034641
rosa_laevigata RLG00000033562
rosa_multiflora Rmu_sc0002460.1_g000087
rosa_roxburghii Rroxscaffold_1G00045680
rosa_rugosa Rorug05G0147400 Rorug05G0147400
rosa_samantha Rh5AG239400 Rh5BG240100 Rh5DG248300
rosa_wichuraiana Rw5G021940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 545
AciI CCGC 3 cut(s) 234, 332, 531
AclWI GGATC 1 cut(s) 305
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 366, 495
AjnI CCWGG 3 cut(s) 250, 266, 494
AleI CACNNNNGTG 1 cut(s) 458
AluBI AGCT 4 cut(s) 13, 71, 437, 504
AluI AGCT 4 cut(s) 13, 71, 437, 504
Alw26I GTCTC 1 cut(s) 545
AlwI GGATC 1 cut(s) 305
AoxI GGCC 2 cut(s) 3, 528
ApeKI GCWGC 2 cut(s) 68, 434
AspS9I GGNCC 1 cut(s) 369
AvaII GGWCC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 2 cut(s) 60, 251
BbsI GAAGAC 1 cut(s) 390
BbvCI CCTCAGC 1 cut(s) 315
BbvI GCAGC 2 cut(s) 80, 446
BciT130I CCWGG 3 cut(s) 252, 268, 496
BcoDI GTCTC 1 cut(s) 545
BfuAI ACCTGC 1 cut(s) 299
BisI GCNGC 3 cut(s) 69, 435, 531
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 3 cut(s) 70, 436, 532
Bme1390I CCNGG 3 cut(s) 252, 268, 496
Bme18I GGWCC 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 2 cut(s) 337, 547
BmrFI CCNGG 3 cut(s) 252, 268, 496
BmsI GCATC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 3 cut(s) 273, 375, 543
Bpu10I CCTNAGC 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 3 cut(s) 250, 267, 495
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 495
Bse1I ACTGG 1 cut(s) 526
Bse3DI GCAATG 2 cut(s) 147, 309
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 3 cut(s) 252, 268, 496
BseDI CCNNGG 3 cut(s) 250, 267, 495
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 366, 495
BseMI GCAATG 2 cut(s) 147, 309
BseMII CTCAG 4 cut(s) 63, 156, 209, 306
BseNI ACTGG 1 cut(s) 526
BseRI GAGGAG 2 cut(s) 94, 270
BseXI GCAGC 2 cut(s) 80, 446
BshFI GGCC 2 cut(s) 5, 530
BshNI GGYRCC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 366, 495
BsmAI GTCTC 1 cut(s) 545
BsnI GGCC 2 cut(s) 5, 530
Bso31I GGTCTC 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 60, 251
Bsp143I GATC 2 cut(s) 21, 297
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 3 cut(s) 234, 332, 531
BspANI GGCC 2 cut(s) 5, 530
BspCNI CTCAG 4 cut(s) 64, 155, 208, 307
BspLI GGNNCC 2 cut(s) 337, 547
BspMI ACCTGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 545
BsrDI GCAATG 2 cut(s) 147, 309
BsrI ACTGG 1 cut(s) 526
BssECI CCNNGG 3 cut(s) 250, 267, 495
BssMI GATC 2 cut(s) 21, 297
Bst2UI CCWGG 3 cut(s) 252, 268, 496
Bst4CI ACNGT 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 60, 326, 330, 502
BstDEI CTNAG 4 cut(s) 72, 142, 195, 315
BstKTI GATC 2 cut(s) 24, 300
BstMAI GTCTC 1 cut(s) 545
BstMBI GATC 2 cut(s) 21, 297
BstMWI GCNNNNNNNGC 3 cut(s) 68, 231, 434
BstNI CCWGG 3 cut(s) 252, 268, 496
BstSCI CCNGG 3 cut(s) 250, 266, 494
BstV1I GCAGC 2 cut(s) 80, 446
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BsuRI GGCC 2 cut(s) 5, 530
BtgZI GCGATG 1 cut(s) 31
BveI ACCTGC 1 cut(s) 299
Cac8I GCNNGC 4 cut(s) 60, 326, 330, 502
Cfr13I GGNCC 1 cut(s) 369
Csp6I GTAC 1 cut(s) 364
CviAII CATG 1 cut(s) 89
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 4 cut(s) 72, 142, 195, 315
DpnI GATC 2 cut(s) 23, 299
DpnII GATC 2 cut(s) 21, 297
DraI TTTAAA 1 cut(s) 553
Eco24I GRGCYC 2 cut(s) 60, 251
Eco31I GGTCTC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 369
EcoRII CCWGG 3 cut(s) 250, 266, 494
EcoT38I GRGCYC 2 cut(s) 60, 251
FaeI CATG 1 cut(s) 92
FaiI YATR 4 cut(s) 90, 138, 287, 514
FatI CATG 1 cut(s) 88
FauI CCCGC 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 69, 435, 531
FriOI GRGCYC 2 cut(s) 60, 251
Fsp4HI GCNGC 3 cut(s) 69, 435, 531
GluI GCNGC 3 cut(s) 69, 435, 531
GsuI CTGGAG 3 cut(s) 273, 375, 543
HaeIII GGCC 2 cut(s) 5, 530
Hin1II CATG 1 cut(s) 92
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 502
HinfI GANTC 1 cut(s) 140
HpaI GTTAAC 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 3 cut(s) 392, 448, 489
Hpy8I GTNNAC 1 cut(s) 40
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 4 cut(s) 68, 92, 103, 152
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 231, 434
HpyF3I CTNAG 4 cut(s) 72, 142, 195, 315
Hsp92II CATG 1 cut(s) 92
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 21, 297
LmnI GCTCC 3 cut(s) 170, 257, 431
Lsp1109I GCAGC 2 cut(s) 80, 446
LweI GCATC 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 344
MalI GATC 2 cut(s) 23, 299
MboI GATC 2 cut(s) 21, 297
MboII GAAGA 2 cut(s) 298, 395
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 2 cut(s) 60, 251
MluCI AATT 1 cut(s) 456
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 8 cut(s) 16, 72, 194, 248, 310, 399, 475, 520
MseI TTAA 2 cut(s) 39, 552
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 2 cut(s) 71, 533
MspR9I CCNGG 3 cut(s) 252, 268, 496
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 3 cut(s) 252, 268, 496
MwoI GCNNNNNNNGC 3 cut(s) 68, 231, 434
NdeII GATC 2 cut(s) 21, 297
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 2 cut(s) 337, 547
NmeAIII GCCGAG 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 458
PkrI GCNGC 3 cut(s) 70, 436, 532
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
Psp6I CCWGG 3 cut(s) 250, 266, 494
PspGI CCWGG 3 cut(s) 250, 266, 494
PspN4I GGNNCC 2 cut(s) 337, 547
PspPI GGNCC 1 cut(s) 369
PsuI RGATCY 1 cut(s) 297
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 2 cut(s) 39, 552
SatI GCNGC 3 cut(s) 69, 435, 531
Sau3AI GATC 2 cut(s) 21, 297
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 3 cut(s) 252, 268, 496
SduI GDGCHC 2 cut(s) 60, 251
SetI ASST 8 cut(s) 15, 73, 201, 293, 321, 439, 506, 551
SfaNI GCATC 1 cut(s) 212
SinI GGWCC 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 458
Sse9I AATT 1 cut(s) 456
SsiI CCGC 3 cut(s) 234, 332, 531
StyD4I CCNGG 3 cut(s) 250, 266, 494
TaaI ACNGT 1 cut(s) 164
TasI AATT 1 cut(s) 456
TatI WGTACW 1 cut(s) 363
TauI GCSGC 1 cut(s) 533
Tru1I TTAA 2 cut(s) 39, 552
Tru9I TTAA 2 cut(s) 39, 552
TseI GCWGC 2 cut(s) 68, 434
TspDTI ATGAA 4 cut(s) 126, 274, 298, 430
TspGWI ACGGA 2 cut(s) 328, 531
VpaK11BI GGWCC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.