Rroxscaffold_1G00045680

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014323
Physical Location & Seq
Forward (+)
64840445 .. 64842134
1690 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00045680.1

Sequence Viewer

Length: 450 bp
ATGGCCTCAAAGCTGTTGTTGATCGCAATCGTCATCGTTAACATCATCGCTTTTGGGCTCGCTGTTGCAGCTGAGCAGAGGAGAAGCCATGTCATTGTAATGTTGGTAAGCCGATGCTTTTGTTGTGGAAAGCCATTGAGCCCTGGAGGAGCAAGAGCCTGGGCAGTCGTCCTTTTCATAACCTGCTGGATCTTTTTCTTCATTGCTGAGGCGTGCTTGCTGGCGGGTTCCGTGAGTAACGCCTACCACACCAAGTACAGGACCATTTTCAGCGAAAACCCTCCAGATTGCCAGACATTGAGAAAGGGAGTTTTTGGGGCAGGAGCAGCTTTCATTTTCCTGAACACAATTGTGTCCACGTTCTACTACACTAACTATTCCAGAGCCAGGGCAAGCTTTCAGTCATATGGAACTGGAGAGGGCGCTGTTGGTCTCGGCACCTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

149

Amino Acids

16.17

Weight (kDa)

9.47

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 29 - 116 3.4e-29 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27435
fragaria_vesca FvH4_3g20620
malus_domestica MD03G1223600.v1.1 MD11G1241500.v1.1
prunus_persica Prupe.4G185900_v2.0.a1
pyrus_communis pycom03g17190 pycom11g21310
rosa_chinensis RchiOBHm_Chr5g0034641
rosa_laevigata RLG00000033562
rosa_multiflora Rmu_sc0002460.1_g000087
rosa_roxburghii Rroxscaffold_1G00045680
rosa_rugosa Rorug05G0147400 Rorug05G0147400
rosa_samantha Rh5AG239400 Rh5BG240100 Rh5DG248300
rosa_wichuraiana Rw5G021940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 437
AciI CCGC 1 cut(s) 224
AclWI GGATC 1 cut(s) 197
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 258, 387
AjnI CCWGG 3 cut(s) 142, 158, 386
AleI CACNNNNGTG 1 cut(s) 350
AluBI AGCT 4 cut(s) 13, 71, 329, 396
AluI AGCT 4 cut(s) 13, 71, 329, 396
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 1 cut(s) 197
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 68, 326
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 261
AvaII GGWCC 1 cut(s) 261
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 2 cut(s) 60, 143
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 80, 338
BciT130I CCWGG 3 cut(s) 144, 160, 388
BcoDI GTCTC 1 cut(s) 437
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 191
BisI GCNGC 2 cut(s) 69, 327
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 2 cut(s) 70, 328
Bme1390I CCNGG 3 cut(s) 144, 160, 388
Bme18I GGWCC 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 229, 439
BmrFI CCNGG 3 cut(s) 144, 160, 388
BmsI GCATC 1 cut(s) 104
BpmI CTGGAG 3 cut(s) 165, 267, 435
Bpu10I CCTNAGC 1 cut(s) 207
Bpu1102I GCTNAGC 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 3 cut(s) 142, 159, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 387
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 201
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 3 cut(s) 144, 160, 388
BseDI CCNNGG 3 cut(s) 142, 159, 387
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 258, 387
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 2 cut(s) 63, 198
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 2 cut(s) 94, 162
BseXI GCAGC 2 cut(s) 80, 338
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 437
BslI CCNNNNNNNGG 2 cut(s) 258, 387
BsmAI GTCTC 1 cut(s) 437
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 60, 143
Bsp143I GATC 2 cut(s) 21, 189
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 1 cut(s) 224
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 64, 199
BspLI GGNNCC 2 cut(s) 229, 439
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 3 cut(s) 142, 159, 387
BssMI GATC 2 cut(s) 21, 189
Bst2UI CCWGG 3 cut(s) 144, 160, 388
BstC8I GCNNGC 5 cut(s) 60, 214, 218, 222, 394
BstDEI CTNAG 2 cut(s) 72, 207
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 2 cut(s) 24, 192
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 2 cut(s) 21, 189
BstMWI GCNNNNNNNGC 2 cut(s) 68, 326
BstNI CCWGG 3 cut(s) 144, 160, 388
BstSCI CCNGG 3 cut(s) 142, 158, 386
BstV1I GCAGC 2 cut(s) 80, 338
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 31
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 5 cut(s) 60, 214, 218, 222, 394
CfoI GCGC 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 261
Csp6I GTAC 1 cut(s) 256
CviAII CATG 1 cut(s) 89
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 2 cut(s) 72, 207
DpnI GATC 2 cut(s) 23, 191
DpnII GATC 2 cut(s) 21, 189
DraI TTTAAA 1 cut(s) 445
Eco24I GRGCYC 2 cut(s) 60, 143
Eco31I GGTCTC 1 cut(s) 437
Eco47I GGWCC 1 cut(s) 261
EcoRII CCWGG 3 cut(s) 142, 158, 386
EcoT38I GRGCYC 2 cut(s) 60, 143
FaeI CATG 1 cut(s) 92
FaiI YATR 4 cut(s) 90, 179, 406, 408
FatI CATG 1 cut(s) 88
FauI CCCGC 1 cut(s) 217
FauNDI CATATG 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 69, 327
FriOI GRGCYC 2 cut(s) 60, 143
Fsp4HI GCNGC 2 cut(s) 69, 327
GlaI GCGC 1 cut(s) 424
GluI GCNGC 2 cut(s) 69, 327
GsuI CTGGAG 3 cut(s) 165, 267, 435
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 425
Hin1II CATG 1 cut(s) 92
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 394
HpaI GTTAAC 1 cut(s) 40
Hpy166II GTNNAC 2 cut(s) 40, 357
Hpy188III TCNNGA 3 cut(s) 284, 340, 381
Hpy8I GTNNAC 2 cut(s) 40, 357
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 326
HpyF3I CTNAG 2 cut(s) 72, 207
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 1 cut(s) 92
HspAI GCGC 1 cut(s) 423
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 21, 189
LmnI GCTCC 2 cut(s) 149, 323
Lsp1109I GCAGC 2 cut(s) 80, 338
LweI GCATC 1 cut(s) 104
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 236
MalI GATC 2 cut(s) 23, 191
MboI GATC 2 cut(s) 21, 189
MboII GAAGA 1 cut(s) 190
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 2 cut(s) 60, 143
MluCI AATT 1 cut(s) 348
MnlI CCTC 6 cut(s) 16, 72, 140, 202, 291, 412
MseI TTAA 2 cut(s) 39, 444
MslI CAYNNNNRTG 2 cut(s) 98, 350
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 3 cut(s) 144, 160, 388
MunI CAATTG 1 cut(s) 348
MvaI CCWGG 3 cut(s) 144, 160, 388
MwoI GCNNNNNNNGC 2 cut(s) 68, 326
NdeI CATATG 1 cut(s) 406
NdeII GATC 2 cut(s) 21, 189
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 2 cut(s) 229, 439
NmeAIII GCCGAG 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 350
PkrI GCNGC 2 cut(s) 70, 328
Psp6I CCWGG 3 cut(s) 142, 158, 386
PspGI CCWGG 3 cut(s) 142, 158, 386
PspN4I GGNNCC 2 cut(s) 229, 439
PspPI GGNCC 1 cut(s) 261
PsuI RGATCY 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 2 cut(s) 98, 350
SaqAI TTAA 2 cut(s) 39, 444
SatI GCNGC 2 cut(s) 69, 327
Sau3AI GATC 2 cut(s) 21, 189
Sau96I GGNCC 1 cut(s) 261
ScrFI CCNGG 3 cut(s) 144, 160, 388
SduI GDGCHC 2 cut(s) 60, 143
SetI ASST 7 cut(s) 15, 73, 185, 331, 362, 398, 443
SfaNI GCATC 1 cut(s) 104
SinI GGWCC 1 cut(s) 261
SmiMI CAYNNNNRTG 2 cut(s) 98, 350
Sse9I AATT 1 cut(s) 348
SsiI CCGC 1 cut(s) 224
StyD4I CCNGG 3 cut(s) 142, 158, 386
TaiI ACGT 1 cut(s) 362
TasI AATT 1 cut(s) 348
TatI WGTACW 1 cut(s) 255
Tru1I TTAA 2 cut(s) 39, 444
Tru9I TTAA 2 cut(s) 39, 444
TseI GCWGC 2 cut(s) 68, 326
TspDTI ATGAA 3 cut(s) 166, 190, 322
TspGWI ACGGA 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.