MD03G1223600.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014323
Physical Location & Seq
Forward (+)
30867671 .. 30870648
2978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1223600.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGCATCCAAGCTGTTGCTGATGACAGTGTTCATCCTGAATGTCATTGCTTTTGGGCTGGCTGTTGCAGCTGAGCAGAGGAGAAGCTCTGCAACGGTTGTTCAGGATACTGAGTTAAACAACTATTGTGTCTATGAGTCAGACATCGCAACTGGCTACGGGGTTGGTGCATTTTTATTCTTCTTGGTAGGTCAGGTCATTATAATGGTGGCAAGCCGATGCTTCTGCTGCGGGAAGCCATTGAAACCTGGAGGGTCAAGAGCCTGGGCAGTTATCCTTTTCATAGTCTGCTGGGTCTTTTTCTTAATCGCTGAGGCGTGTTTGCTGGCGGGTTCGGTGAGGAATGCCTACCACACCAAGTACAGGACCATTTTCGGCCAAAGCCCTCCAGATTGCCAGCAACTGAGAAAGGGAGTTTTCGGTGCAGGAGCAGCTTTCATTTTCCTCACCACAATAGTTTCTGAGTTCTACTATGTTTGCTATTCCAGAGCCAGGGAAAGCTTCCAGCCATACGGCGGAGAGGCTGCTGTTGGTCTCGGAACCTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

184

Amino Acids

20.0

Weight (kDa)

8.8

Isoelectric Point (pI)

32.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 57 - 152 1.4e-31 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27435
fragaria_vesca FvH4_3g20620
malus_domestica MD03G1223600.v1.1 MD11G1241500.v1.1
prunus_persica Prupe.4G185900_v2.0.a1
pyrus_communis pycom03g17190 pycom11g21310
rosa_chinensis RchiOBHm_Chr5g0034641
rosa_laevigata RLG00000033562
rosa_multiflora Rmu_sc0002460.1_g000087
rosa_roxburghii Rroxscaffold_1G00045680
rosa_rugosa Rorug05G0147400 Rorug05G0147400
rosa_samantha Rh5AG239400 Rh5BG240100 Rh5DG248300
rosa_wichuraiana Rw5G021940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AciI CCGC 3 cut(s) 231, 329, 516
AcoI YGGCCR 1 cut(s) 376
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 363, 492, 515
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 3 cut(s) 247, 263, 491
AluBI AGCT 5 cut(s) 13, 71, 87, 434, 501
AluI AGCT 5 cut(s) 13, 71, 87, 434, 501
Alw26I GTCTC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 403
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 4 cut(s) 68, 228, 431, 524
AspS9I GGNCC 1 cut(s) 366
AsuHPI GGTGA 2 cut(s) 349, 439
AvaII GGWCC 1 cut(s) 366
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 80, 215, 443, 511
BceAI ACGGC 1 cut(s) 529
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 3 cut(s) 249, 265, 493
BciVI GTATCC 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 539
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 4 cut(s) 69, 229, 432, 525
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 4 cut(s) 70, 230, 433, 526
Bme1390I CCNGG 3 cut(s) 249, 265, 493
Bme18I GGWCC 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 1 cut(s) 541
BmrFI CCNGG 3 cut(s) 249, 265, 493
BmsI GCATC 2 cut(s) 14, 209
BpmI CTGGAG 2 cut(s) 270, 372
Bpu10I CCTNAGC 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 264, 492
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 492, 515
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 3 cut(s) 249, 265, 493
BseDI CCNNGG 2 cut(s) 264, 492
BseGI GGATG 2 cut(s) 5, 33
BseLI CCNNNNNNNGG 3 cut(s) 363, 492, 515
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 5 cut(s) 63, 102, 303, 395, 453
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 4 cut(s) 80, 215, 443, 511
BseYI CCCAGC 1 cut(s) 291
BsgI GTGCAG 1 cut(s) 444
BshFI GGCC 1 cut(s) 378
BslI CCNNNNNNNGG 3 cut(s) 363, 492, 515
BsmAI GTCTC 1 cut(s) 539
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 1 cut(s) 378
Bso31I GGTCTC 1 cut(s) 539
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 3 cut(s) 231, 329, 516
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 5 cut(s) 64, 103, 304, 396, 454
BspLI GGNNCC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 264, 492
Bst2UI CCWGG 3 cut(s) 249, 265, 493
Bst4CI ACNGT 2 cut(s) 28, 97
BstC8I GCNNGC 4 cut(s) 60, 214, 327, 398
BstDEI CTNAG 5 cut(s) 72, 111, 312, 404, 462
BstF5I GGATG 2 cut(s) 5, 33
BstMAI GTCTC 1 cut(s) 539
BstMWI GCNNNNNNNGC 3 cut(s) 68, 228, 431
BstNI CCWGG 3 cut(s) 249, 265, 493
BstSCI CCNGG 3 cut(s) 247, 263, 491
BstV1I GCAGC 4 cut(s) 80, 215, 443, 511
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 2 cut(s) 5, 33
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 4 cut(s) 60, 214, 327, 398
CaiI CAGNNNCTG 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 366
Csp6I GTAC 1 cut(s) 361
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 5 cut(s) 72, 111, 312, 404, 462
DraI TTTAAA 1 cut(s) 547
EaeI YGGCCR 1 cut(s) 376
EciI GGCGGA 1 cut(s) 531
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 366
EcoRII CCWGG 3 cut(s) 247, 263, 491
FaiI YATR 5 cut(s) 135, 203, 284, 474, 511
FauI CCCGC 2 cut(s) 224, 322
Fnu4HI GCNGC 4 cut(s) 69, 229, 432, 525
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 4 cut(s) 69, 229, 432, 525
GluI GCNGC 4 cut(s) 69, 229, 432, 525
GsaI CCCAGC 1 cut(s) 295
GsuI CTGGAG 2 cut(s) 270, 372
HaeIII GGCC 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 1 cut(s) 137
HphI GGTGA 2 cut(s) 349, 439
Hpy188I TCNGA 3 cut(s) 142, 463, 539
Hpy188III TCNNGA 5 cut(s) 37, 104, 258, 389, 486
HpyCH4III ACNGT 2 cut(s) 28, 97
HpyCH4V TGCA 4 cut(s) 68, 92, 170, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 228, 431
HpyF3I CTNAG 5 cut(s) 72, 111, 312, 404, 462
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 4 cut(s) 80, 215, 443, 511
LweI GCATC 2 cut(s) 14, 209
MboII GAAGA 1 cut(s) 172
MlyI GAGTC 1 cut(s) 146
MnlI CCTC 7 cut(s) 72, 245, 307, 333, 396, 455, 514
MseI TTAA 3 cut(s) 116, 305, 546
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 3 cut(s) 249, 265, 493
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 3 cut(s) 249, 265, 493
MwoI GCNNNNNNNGC 3 cut(s) 68, 228, 431
NlaIV GGNNCC 1 cut(s) 541
PctI GAATGC 1 cut(s) 349
PkrI GCNGC 4 cut(s) 70, 230, 433, 526
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 247, 263, 491
PspFI CCCAGC 1 cut(s) 291
PspGI CCWGG 3 cut(s) 247, 263, 491
PspN4I GGNNCC 1 cut(s) 541
PspPI GGNCC 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 3 cut(s) 116, 305, 546
SatI GCNGC 4 cut(s) 69, 229, 432, 525
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 3 cut(s) 249, 265, 493
SetI ASST 9 cut(s) 15, 73, 89, 193, 198, 250, 436, 503, 545
SfaNI GCATC 2 cut(s) 14, 209
SinI GGWCC 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 203
SsiI CCGC 3 cut(s) 231, 329, 516
StyD4I CCNGG 3 cut(s) 247, 263, 491
TaaI ACNGT 2 cut(s) 28, 97
TatI WGTACW 1 cut(s) 360
Tru1I TTAA 3 cut(s) 116, 305, 546
Tru9I TTAA 3 cut(s) 116, 305, 546
TscAI CASTG 1 cut(s) 33
TseI GCWGC 4 cut(s) 68, 228, 431, 524
TspDTI ATGAA 3 cut(s) 22, 271, 427
TspRI CASTG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.