AT4G30590

early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016542
Physical Location & Seq
Reverse (-)
14935584 .. 14936562
979 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30590.1

Sequence Viewer

Length: 573 bp
ATGGGAATCATAGTTCCAGTTTTGACCCTTGTCTTCCTCTTGTTTGCTAAAGTGAGCCATGGTGCCTCCAATCCTAGGGTGATTCTAGTTGGTGGATCTGTGGGATCATGGAAGGTTCCTGATTCTCCTAACAACACTCTCAATCACTGGGCCGAGAACAACCGTTTCAAAGTCGGCGACTTCATCGTGTGGAAGTACGACATGAAAGTAGATTCGGTACTACAAGTGACAAAAGAAGATTACGAGAGCTGTAACACAGCAAATCCCTTGAAGCAATACAATGATGGAAACACTAAGGTTGCGCTTGACAAATCTGGCCCTTACTTCTTCATTAGTGGTGCTCCTGGTAACTGCGCTAAAGGCGAAAAGATTACCCTTGTTGTTTTGGCTGAACGCAAATCCGGTGGTGGCAGCAGCAGCGGTGACGCTCCTAAGGTTTCTCCTGTTAGTCCTACGGCTCAAACTCCAGCTCCAGCTCCCGGTCCAGCTGCAGCCCATAACGCTGCGGTGGGGTTGAAGGTTGCAAGCGGTTGGTTCTTGACCGCAGTCGTGGTTGGTTTGGCTATGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

190

Amino Acids

19.82

Weight (kDa)

9.08

Isoelectric Point (pI)

26.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 121 1.1e-26 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30590
fragaria_vesca FvH4_1g00840
malus_domestica MD02G1006700.v1.1
prunus_persica Prupe.7G263900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0085571
rosa_laevigata RLG00000015701
rosa_multiflora Rmu_ssc0000347.1_g000005
rosa_roxburghii Rroxscaffold_2G00155260
rosa_rugosa Rorug01G0461400
rosa_samantha Rh2AG010300 Rh2BG010900 Rh2CG011800 Rh2DG012800
rosa_wichuraiana Rw2G000920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AciI CCGC 4 cut(s) 420, 506, 528, 543
AclWI GGATC 2 cut(s) 103, 112
AfaI GTAC 2 cut(s) 197, 219
AfiI CCNNNNNNNGG 2 cut(s) 75, 479
AgsI TTSAA 3 cut(s) 169, 271, 517
AjnI CCWGG 1 cut(s) 343
AloI GAACNNNNNNTCC 1 cut(s) 29
AluBI AGCT 4 cut(s) 249, 470, 476, 488
AluI AGCT 4 cut(s) 249, 470, 476, 488
Alw21I GWGCWC 1 cut(s) 343
AlwI GGATC 2 cut(s) 103, 112
AoxI GGCC 2 cut(s) 150, 316
ApeKI GCWGC 6 cut(s) 411, 414, 417, 488, 491, 503
AspA2I CCTAGG 1 cut(s) 74
AspLEI GCGC 2 cut(s) 304, 356
AspS9I GGNCC 3 cut(s) 150, 317, 482
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 2 cut(s) 91, 434
AvaII GGWCC 1 cut(s) 482
AvrII CCTAGG 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 432
BanI GGYRCC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 6 cut(s) 423, 426, 429, 475, 490, 503
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 345
BcnI CCSGG 1 cut(s) 480
BfaI CTAG 2 cut(s) 75, 86
BfmI CTRYAG 1 cut(s) 489
BisI GCNGC 6 cut(s) 412, 415, 418, 489, 492, 504
BlnI CCTAGG 1 cut(s) 74
BlsI GCNGC 6 cut(s) 413, 416, 419, 490, 493, 505
Bme1390I CCNGG 2 cut(s) 345, 480
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 3 cut(s) 150, 317, 482
BmiI GGNNCC 2 cut(s) 64, 117
BmrFI CCNGG 2 cut(s) 345, 480
BmrI ACTGGG 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 157
BoxI GACNNNNGTC 2 cut(s) 29, 545
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 2 cut(s) 450, 456
BpuMI CCSGG 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 58, 74
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 479
Bse1I ACTGG 2 cut(s) 17, 152
Bse21I CCTNAGG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 2 cut(s) 58, 74
BseLI CCNNNNNNNGG 2 cut(s) 75, 479
BseNI ACTGG 2 cut(s) 17, 152
BseXI GCAGC 6 cut(s) 423, 426, 429, 475, 490, 503
BshFI GGCC 2 cut(s) 152, 318
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 2 cut(s) 402, 480
BslI CCNNNNNNNGG 2 cut(s) 75, 479
BsnI GGCC 2 cut(s) 152, 318
Bsp1286I GDGCHC 1 cut(s) 343
Bsp143I GATC 2 cut(s) 95, 104
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 4 cut(s) 420, 506, 528, 543
BspANI GGCC 2 cut(s) 152, 318
BspLI GGNNCC 2 cut(s) 64, 117
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 2 cut(s) 103, 112
BspT107I GGYRCC 1 cut(s) 62
BsrI ACTGG 2 cut(s) 17, 152
BssECI CCNNGG 2 cut(s) 58, 74
BssMI GATC 2 cut(s) 95, 104
BssT1I CCWWGG 2 cut(s) 58, 74
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 3 cut(s) 294, 432, 570
BstDSI CCRYGG 1 cut(s) 58
BstHHI GCGC 2 cut(s) 304, 356
BstKTI GATC 2 cut(s) 98, 107
BstMBI GATC 2 cut(s) 95, 104
BstMWI GCNNNNNNNGC 3 cut(s) 360, 417, 500
BstNI CCWGG 1 cut(s) 345
BstPAI GACNNNNGTC 2 cut(s) 29, 545
BstSCI CCNGG 2 cut(s) 343, 478
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 6 cut(s) 423, 426, 429, 475, 490, 503
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
Bsu36I CCTNAGG 1 cut(s) 432
BsuRI GGCC 2 cut(s) 152, 318
BtgI CCRYGG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 526
CfoI GCGC 2 cut(s) 304, 356
Cfr13I GGNCC 3 cut(s) 150, 317, 482
CseI GACGC 1 cut(s) 434
Csp6I GTAC 2 cut(s) 196, 218
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 3 cut(s) 59, 108, 202
CviQI GTAC 2 cut(s) 196, 218
DdeI CTNAG 3 cut(s) 294, 432, 570
DpnI GATC 2 cut(s) 97, 106
DpnII GATC 2 cut(s) 95, 104
Eco130I CCWWGG 2 cut(s) 58, 74
Eco47I GGWCC 1 cut(s) 482
Eco81I CCTNAGG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 58, 74
ErhI CCWWGG 2 cut(s) 58, 74
FaeI CATG 3 cut(s) 62, 111, 205
FaiI YATR 6 cut(s) 11, 60, 109, 203, 498, 566
FatI CATG 3 cut(s) 58, 107, 201
Fnu4HI GCNGC 6 cut(s) 412, 415, 418, 489, 492, 504
Fsp4HI GCNGC 6 cut(s) 412, 415, 418, 489, 492, 504
FspBI CTAG 2 cut(s) 75, 86
GlaI GCGC 2 cut(s) 303, 355
GluI GCNGC 6 cut(s) 412, 415, 418, 489, 492, 504
GsuI CTGGAG 2 cut(s) 450, 456
HaeIII GGCC 2 cut(s) 152, 318
HapII CCGG 2 cut(s) 402, 480
HgaI GACGC 1 cut(s) 434
HhaI GCGC 2 cut(s) 304, 356
Hin1II CATG 3 cut(s) 62, 111, 205
Hin6I GCGC 2 cut(s) 302, 354
HinP1I GCGC 2 cut(s) 302, 354
HinfI GANTC 4 cut(s) 6, 82, 122, 212
HpaII CCGG 2 cut(s) 402, 480
HphI GGTGA 2 cut(s) 91, 434
Hpy188III TCNNGA 2 cut(s) 119, 538
HpyAV CCTTC 2 cut(s) 106, 511
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4V TGCA 2 cut(s) 491, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 360, 417, 500
HpyF3I CTNAG 3 cut(s) 294, 432, 570
Hsp92II CATG 3 cut(s) 62, 111, 205
HspAI GCGC 2 cut(s) 302, 354
Kzo9I GATC 2 cut(s) 95, 104
LmnI GCTCC 4 cut(s) 346, 433, 475, 481
Lsp1109I GCAGC 6 cut(s) 423, 426, 429, 475, 490, 503
MaeI CTAG 2 cut(s) 75, 86
MaeIII GTNAC 4 cut(s) 226, 251, 347, 422
MalI GATC 2 cut(s) 97, 106
MboI GATC 2 cut(s) 95, 104
MboII GAAGA 3 cut(s) 25, 248, 319
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 343
MnlI CCTC 2 cut(s) 47, 76
MspA1I CMGCKG 2 cut(s) 420, 488
MspI CCGG 2 cut(s) 402, 480
MspR9I CCNGG 2 cut(s) 345, 480
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 360, 417, 500
NciI CCSGG 1 cut(s) 480
NcoI CCATGG 1 cut(s) 58
NdeII GATC 2 cut(s) 95, 104
NlaIII CATG 3 cut(s) 62, 111, 205
NlaIV GGNNCC 2 cut(s) 64, 117
NmeAIII GCCGAG 1 cut(s) 178
NmuCI GTSAC 2 cut(s) 226, 422
PfeI GAWTC 4 cut(s) 6, 82, 122, 212
PkrI GCNGC 6 cut(s) 413, 416, 419, 490, 493, 505
PshAI GACNNNNGTC 2 cut(s) 29, 545
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 2 cut(s) 64, 117
PspPI GGNCC 3 cut(s) 150, 317, 482
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 2 cut(s) 197, 219
RsaNI GTAC 2 cut(s) 196, 218
SatI GCNGC 6 cut(s) 412, 415, 418, 489, 492, 504
Sau3AI GATC 2 cut(s) 95, 104
Sau96I GGNCC 3 cut(s) 150, 317, 482
ScrFI CCNGG 2 cut(s) 345, 480
SduI GDGCHC 1 cut(s) 343
SetI ASST 8 cut(s) 117, 251, 300, 438, 472, 478, 490, 522
SfcI CTRYAG 1 cut(s) 489
SinI GGWCC 1 cut(s) 482
SsiI CCGC 4 cut(s) 420, 506, 528, 543
SspMI CTAG 2 cut(s) 75, 86
StyD4I CCNGG 2 cut(s) 343, 478
StyI CCWWGG 2 cut(s) 58, 74
TaaI ACNGT 1 cut(s) 164
TfiI GAWTC 4 cut(s) 6, 82, 122, 212
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 2 cut(s) 226, 422
TseI GCWGC 6 cut(s) 411, 414, 417, 488, 491, 503
Tsp45I GTSAC 2 cut(s) 226, 422
TspDTI ATGAA 3 cut(s) 172, 218, 319
TspRI CASTG 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 482
XmaJI CCTAGG 1 cut(s) 74
XspI CTAG 2 cut(s) 75, 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.