MD02G1006700.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016542
Physical Location & Seq
Forward (+)
456774 .. 457658
885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1006700.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCTTCCTCGAGAAATATTGGTTTTGCAGTTTTGTCCTTTGTCTTTGTGTTATTTGCTTTCTCCGAAGCCAAAGTCATTCTGGTTGGAGATAAGGACGTTTCTTGGAACGTTGATTCTGTCAAGGCCTTGACTCAGTGGGCTGAGAATAGACGATTCAGAATCGGATATGTTCTCAAATGGAAAAAGGAGGAAGGGAGGGACTCCGTGGTGCAAGTGACAAAGGAAGCCTATGAGACCTGCGACACATCGAAGCCAATCAAAGCAGATGTGGAGGAGATTACGCTGGACAGGTCAGGACCATTCTACTTCATCAGTGGTGTTAAGGAGCACTGTGACAAAGGCTTGAAGCTCACGGTGGTGGTTCTGACGGAAAAACCTAAATATGGTTCGTACTCTCCGTTGCCTGCTCCGGCTGCACCGCCGTCTAGTAATGCTGCTGCCCCTGGTTTCGGTTTGAGTGCTGGATTTATTGCGGTTGTGGTGGCAGTGGGGATGGCTTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

170

Amino Acids

18.32

Weight (kDa)

8.49

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 36 - 115 1.1e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30590
fragaria_vesca FvH4_1g00840
malus_domestica MD02G1006700.v1.1
prunus_persica Prupe.7G263900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0085571
rosa_laevigata RLG00000015701
rosa_multiflora Rmu_ssc0000347.1_g000005
rosa_roxburghii Rroxscaffold_2G00155260
rosa_rugosa Rorug01G0461400
rosa_samantha Rh2AG010300 Rh2BG010900 Rh2CG011800 Rh2DG012800
rosa_wichuraiana Rw2G000920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 248
AciI CCGC 2 cut(s) 422, 476
AclI AACGTT 1 cut(s) 111
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 2 cut(s) 386, 452
AgsI TTSAA 1 cut(s) 349
AjnI CCWGG 1 cut(s) 445
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 1 cut(s) 352
AluI AGCT 1 cut(s) 352
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 3 cut(s) 416, 437, 440
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AspS9I GGNCC 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 403, 424, 427
BccI CCATC 1 cut(s) 490
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 1 cut(s) 429
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 3 cut(s) 417, 438, 441
BlsI GCNGC 3 cut(s) 418, 439, 442
Bme1390I CCNGG 1 cut(s) 447
Bme18I GGWCC 1 cut(s) 299
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 1 cut(s) 299
BmrFI CCNGG 1 cut(s) 447
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 207, 445
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 452
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 207, 445
BseGI GGATG 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 386, 452
BseMII CTCAG 2 cut(s) 135, 149
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 3 cut(s) 403, 424, 427
BsgI GTGCAG 1 cut(s) 402
BshFI GGCC 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 386, 452
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 333
BspACI CCGC 2 cut(s) 422, 476
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 2 cut(s) 136, 148
BspMI ACCTGC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 230
BssECI CCNNGG 2 cut(s) 207, 445
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 2 cut(s) 335, 358
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 2 cut(s) 135, 144
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 501
BstMAI GTCTC 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 1 cut(s) 445
BstV1I GCAGC 3 cut(s) 403, 424, 427
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 1 cut(s) 501
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 4 cut(s) 143, 322, 331, 495
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 394
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 2 cut(s) 135, 144
Eco147I AGGCCT 1 cut(s) 128
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 445
FaiI YATR 3 cut(s) 171, 234, 387
FaqI GGGAC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 417, 438, 441
Fsp4HI GCNGC 3 cut(s) 417, 438, 441
FspBI CTAG 1 cut(s) 429
GluI GCNGC 3 cut(s) 417, 438, 441
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 413
HinfI GANTC 5 cut(s) 116, 133, 156, 162, 203
HpaII CCGG 1 cut(s) 413
Hpy188I TCNGA 4 cut(s) 67, 161, 167, 369
Hpy188III TCNNGA 2 cut(s) 12, 297
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 335, 358
HpyCH4IV ACGT 2 cut(s) 99, 111
HpyCH4V TGCA 3 cut(s) 29, 214, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
HpyF3I CTNAG 2 cut(s) 135, 144
HpySE526I ACGT 2 cut(s) 99, 111
LmnI GCTCC 2 cut(s) 328, 415
Lsp1109I GCAGC 3 cut(s) 403, 424, 427
MaeI CTAG 1 cut(s) 429
MaeII ACGT 2 cut(s) 99, 111
MaeIII GTNAC 2 cut(s) 217, 335
MhlI GDGCHC 1 cut(s) 333
MlyI GAGTC 2 cut(s) 127, 197
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 4 cut(s) 19, 184, 192, 268
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 359
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 447
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 1 cut(s) 416
NmuCI GTSAC 2 cut(s) 217, 335
OliI CACNNNNGTG 1 cut(s) 359
PaeR7I CTCGAG 1 cut(s) 10
PceI AGGCCT 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PfeI GAWTC 3 cut(s) 116, 156, 162
PkrI GCNGC 3 cut(s) 418, 439, 442
PleI GAGTC 2 cut(s) 127, 197
PpsI GAGTC 2 cut(s) 127, 197
Psp1406I AACGTT 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspPI GGNCC 1 cut(s) 299
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 3 cut(s) 417, 438, 441
Sau96I GGNCC 1 cut(s) 299
SchI GAGTC 2 cut(s) 127, 197
ScrFI CCNGG 1 cut(s) 447
SduI GDGCHC 1 cut(s) 333
SetI ASST 6 cut(s) 102, 114, 242, 296, 354, 382
Sfr274I CTCGAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 299
SlaI CTCGAG 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SseBI AGGCCT 1 cut(s) 128
SsiI CCGC 2 cut(s) 422, 476
SspI AATATT 1 cut(s) 19
SspMI CTAG 1 cut(s) 429
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 445
TaaI ACNGT 2 cut(s) 335, 358
TaiI ACGT 2 cut(s) 102, 114
TaqI TCGA 2 cut(s) 11, 251
TfiI GAWTC 3 cut(s) 116, 156, 162
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 4 cut(s) 143, 322, 338, 495
TseFI GTSAC 2 cut(s) 217, 335
TseI GCWGC 3 cut(s) 416, 437, 440
Tsp45I GTSAC 2 cut(s) 217, 335
TspDTI ATGAA 1 cut(s) 301
TspGWI ACGGA 3 cut(s) 196, 386, 390
TspRI CASTG 4 cut(s) 143, 322, 338, 495
VpaK11BI GGWCC 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 10
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.