RLG00000015701

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016542
Physical Location & Seq
Forward (+)
809236 .. 809880
645 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015701

Sequence Viewer

Length: 549 bp
ATGGCTTCCAACACAATCTGCCTTCCAGTTCTGTCCCTACTCTTCCTGCTGTTTGCTCTCTCTGAAGCGAGAGAAGTCACGGTCGAGGGTTGGACAGTCGAAAACTCCGATCATTTGACCAAGTGGACTCACAGACACCATTTCACCACTGGCGATGTTCTCATATGGAAAACGAGCACACCCCTCGGTTTTGAGCCTCCAGTCCTGGAAGTGACAAAGGAGGCGTATGAGAGCTGTGAAATATCAAACCCGCTCAAAGAAAACGTGACGGAGGTGACTTTGAACCCTGCTGGGCTGTTCTACTTTATTAGCGGCGCTAAGGAGCTTTGTGACAAGGGATTGAAGATAACTGTGAAGGTTCATGATGCTCATAAACATTATGGGGCTCCTGCTCCGGCACCGGCTCTGGCTCCGGAGACGAATAATATGGCTGCAGAGCCAGCTGCGCCACCAACCAGTGCTGCACCGCATGGATTGTTGGGGGGTGGTTTTATGGGTTTTGTTGCGATTGGGGTGGCAGGTGTAGTGGGAATGGCTTTGAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

183

Amino Acids

19.36

Weight (kDa)

5.54

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 31 - 113 4e-13 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016542)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30590
fragaria_vesca FvH4_1g00840
malus_domestica MD02G1006700.v1.1
prunus_persica Prupe.7G263900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0085571
rosa_laevigata RLG00000015701
rosa_multiflora Rmu_ssc0000347.1_g000005
rosa_roxburghii Rroxscaffold_2G00155260
rosa_rugosa Rorug01G0461400
rosa_samantha Rh2AG010300 Rh2BG010900 Rh2CG011800 Rh2DG012800
rosa_wichuraiana Rw2G000920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 253
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 3 cut(s) 251, 312, 467
AcuI CTGAAG 1 cut(s) 84
AgsI TTSAA 3 cut(s) 283, 343, 541
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 3 cut(s) 234, 325, 443
AluI AGCT 3 cut(s) 234, 325, 443
Alw21I GWGCWC 1 cut(s) 179
Alw26I GTCTC 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 412
ApeKI GCWGC 3 cut(s) 431, 443, 461
AspLEI GCGC 2 cut(s) 317, 448
AsuHPI GGTGA 2 cut(s) 136, 286
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 388
Bbv12I GWGCWC 1 cut(s) 179
BbvI GCAGC 3 cut(s) 418, 430, 448
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 509
BisI GCNGC 4 cut(s) 313, 432, 444, 462
BlsI GCNGC 4 cut(s) 314, 433, 445, 463
Bme1390I CCNGG 1 cut(s) 206
BmiI GGNNCC 3 cut(s) 387, 399, 411
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 355
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 184
BsaWI WCCGGW 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 4 cut(s) 26, 154, 200, 456
BseAI TCCGGA 1 cut(s) 412
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 184
BseNI ACTGG 4 cut(s) 26, 154, 200, 456
BseXI GCAGC 3 cut(s) 418, 430, 448
BseYI CCCAGC 1 cut(s) 290
BsgI GTGCAG 1 cut(s) 447
Bsh1285I CGRYCG 1 cut(s) 84
BshNI GGYRCC 1 cut(s) 397
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 179
BsiSI CCGG 3 cut(s) 395, 401, 413
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 410
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 179, 388
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 1 cut(s) 109
BspACI CCGC 3 cut(s) 251, 312, 467
BspEI TCCGGA 1 cut(s) 412
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 3 cut(s) 387, 399, 411
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 4 cut(s) 26, 154, 200, 456
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 3 cut(s) 82, 97, 352
Bst6I CTCTTC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 2 cut(s) 317, 448
BstKTI GATC 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 1 cut(s) 109
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 440, 445
BstNI CCWGG 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 3 cut(s) 418, 430, 448
BtgZI GCGATG 1 cut(s) 168
BtsIMutI CAGTG 2 cut(s) 147, 463
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 441
CciI TCATGA 1 cut(s) 361
CfoI GCGC 2 cut(s) 317, 448
Cfr10I RCCGGY 1 cut(s) 400
CviAII CATG 2 cut(s) 362, 470
DdeI CTNAG 1 cut(s) 318
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 204
EcoT38I GRGCYC 1 cut(s) 388
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 2 cut(s) 365, 473
FaiI YATR 9 cut(s) 164, 166, 228, 363, 372, 381, 428, 471, 494
FaqI GGGAC 1 cut(s) 19
FatI CATG 2 cut(s) 361, 469
FauI CCCGC 1 cut(s) 258
FauNDI CATATG 1 cut(s) 164
Fnu4HI GCNGC 4 cut(s) 313, 432, 444, 462
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 4 cut(s) 313, 432, 444, 462
GlaI GCGC 2 cut(s) 316, 447
GluI GCNGC 4 cut(s) 313, 432, 444, 462
GsaI CCCAGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 183
HaeII RGCGCY 1 cut(s) 318
HapII CCGG 3 cut(s) 395, 401, 413
HhaI GCGC 2 cut(s) 317, 448
Hin1II CATG 2 cut(s) 365, 473
Hin6I GCGC 2 cut(s) 315, 446
HinP1I GCGC 2 cut(s) 315, 446
HinfI GANTC 1 cut(s) 127
HpaII CCGG 3 cut(s) 395, 401, 413
HphI GGTGA 2 cut(s) 136, 286
Hpy166II GTNNAC 1 cut(s) 126
Hpy188I TCNGA 2 cut(s) 64, 109
Hpy188III TCNNGA 2 cut(s) 362, 413
Hpy8I GTNNAC 1 cut(s) 126
HpyAV CCTTC 2 cut(s) 32, 349
HpyCH4III ACNGT 3 cut(s) 82, 97, 352
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 2 cut(s) 434, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 445
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 365, 473
HspAI GCGC 2 cut(s) 315, 446
Kpn2I TCCGGA 1 cut(s) 412
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 4 cut(s) 322, 391, 397, 415
Lsp1109I GCAGC 3 cut(s) 418, 430, 448
LweI GCATC 1 cut(s) 355
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 5 cut(s) 76, 211, 265, 274, 329
MalI GATC 1 cut(s) 111
MbiI CCGCTC 1 cut(s) 253
MboI GATC 1 cut(s) 109
MboII GAAGA 2 cut(s) 34, 355
MhlI GDGCHC 2 cut(s) 179, 388
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 2 cut(s) 33, 71
MnlI CCTC 5 cut(s) 79, 194, 207, 214, 265
MroI TCCGGA 1 cut(s) 412
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 3 cut(s) 395, 401, 413
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 440, 445
NdeI CATATG 1 cut(s) 164
NdeII GATC 1 cut(s) 109
NlaIII CATG 2 cut(s) 365, 473
NlaIV GGNNCC 3 cut(s) 387, 399, 411
NmuCI GTSAC 5 cut(s) 76, 211, 265, 274, 329
PagI TCATGA 1 cut(s) 361
PaqCI CACCTGC 1 cut(s) 509
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 204
PkrI GCNGC 4 cut(s) 314, 433, 445, 463
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 204
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 3 cut(s) 387, 399, 411
PstI CTGCAG 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 443
SatI GCNGC 4 cut(s) 313, 432, 444, 462
Sau3AI GATC 1 cut(s) 109
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 2 cut(s) 179, 388
SetI ASST 7 cut(s) 236, 267, 276, 327, 360, 445, 523
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 432
SsiI CCGC 3 cut(s) 251, 312, 467
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 3 cut(s) 82, 97, 352
TaiI ACGT 1 cut(s) 267
TaqI TCGA 2 cut(s) 84, 99
TauI GCSGC 1 cut(s) 315
TscAI CASTG 2 cut(s) 154, 463
TseFI GTSAC 5 cut(s) 76, 211, 265, 274, 329
TseI GCWGC 3 cut(s) 431, 443, 461
Tsp45I GTSAC 5 cut(s) 76, 211, 265, 274, 329
TspDTI ATGAA 1 cut(s) 350
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 2 cut(s) 154, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.