AT4G32210
HSP70 Family

Encodes one of the membrane anchor subunits of the mitochondrial respiratory complex II. The protein is encoded by the nuclear genome but is imported into the mitochondrion. There are two genes that encode this protein, the other is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010847
Physical Location & Seq
Reverse (-)
15556397 .. 15558167
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32210.1

Sequence Viewer

Length: 642 bp
ATGGCTGCCACGGCTCTTTTCCGATCGATTCGTCGACGCGACGTCGTCTCCGCGCCTCTTTCCGTTTACAAATCCCTTGCTGGTAATGCTCAACCTTCCTGGGGTAGTTCATACATTGGTCAGAACTATGCAAGTTTCTCCAGAGCTTTCGGGTCAAAACCTGTTGTGAATGACATTCTTGGTACTGGTTTGGGCACTAACAATGCCATTAGAGAGGAGAGAGAGAAGTCAAAATCTACTGAAGCTGCAATTGTTGGTGCTCAATTGACTCGATCTTTCCGTGCTCTTGATGTGGGAACATCGAAACGATTGTTTTCTACAATCTCAGGGGATATAAAGACAACACAGGAGGAACCAAAAATCAAAAGCTTTCGCCCTTTATCTCCTCACCTTTCTGTTTACCAGCCTCAGATGAACTCCATGCTATCGATTTTCAACAGAATCTCAGGGGTTTACTTGACCGGTGTCACTTTCGCTGGCTACCTTCTCTACCTGAAGATGGGTATGATCTGCCTCACCTACCCGAGTTTCTACCAAGTCCTTTACCATACACAACAGCAACTTCCAGTCATCACCTCGGTTACTGCATTAGCCGCTATTTATCATACTATCAAGAGTACTCACTCACTCTTGACCCATTAA

Protein Analysis

213

Amino Acids

23.45

Weight (kDa)

9.96

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sdh_cyt PF01127 125 - 205 1.3e-09 Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
AccI GTMKAC 1 cut(s) 34
AccII CGCG 2 cut(s) 39, 53
AciI CCGC 2 cut(s) 51, 594
AcuI CTGAAG 2 cut(s) 261, 515
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 2 cut(s) 184, 619
AfiI CCNNNNNNNGG 2 cut(s) 101, 499
AgeI ACCGGT 1 cut(s) 461
AgsI TTSAA 1 cut(s) 436
AhdI GACNNNNNGTC 1 cut(s) 41
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 3 cut(s) 146, 245, 369
AluI AGCT 3 cut(s) 146, 245, 369
Alw21I GWGCWC 2 cut(s) 262, 286
Alw26I GTCTC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 5, 245
AsiGI ACCGGT 1 cut(s) 461
AspLEI GCGC 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 380, 508, 565
AvaI CYCGRG 1 cut(s) 523
BaeGI GKGCMC 1 cut(s) 197
Bbv12I GWGCWC 2 cut(s) 262, 286
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 493
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 52
BisI GCNGC 3 cut(s) 6, 246, 594
BlsI GCNGC 3 cut(s) 7, 247, 595
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 1 cut(s) 100
BmeRI GACNNNNNGTC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 523
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 464
BpmI CTGGAG 1 cut(s) 124
Bsa29I ATCGAT 2 cut(s) 26, 428
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 3 cut(s) 9, 99, 576
BsaWI WCCGGW 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 499
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 2 cut(s) 190, 566
BseBI CCWGG 1 cut(s) 100
BseCI ATCGAT 2 cut(s) 26, 428
BseDI CCNNGG 3 cut(s) 9, 99, 576
BseLI CCNNNNNNNGG 2 cut(s) 101, 499
BseMII CTCAG 3 cut(s) 339, 422, 459
BseNI ACTGG 2 cut(s) 190, 566
BseRI GAGGAG 2 cut(s) 230, 375
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 1 cut(s) 232
Bsh1236I CGCG 2 cut(s) 39, 53
Bsh1285I CGRYCG 1 cut(s) 26
BshTI ACCGGT 1 cut(s) 461
BshVI ATCGAT 2 cut(s) 26, 428
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 2 cut(s) 262, 286
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 1 cut(s) 462
BslI CCNNNNNNNGG 2 cut(s) 101, 499
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 523
Bsp1286I GDGCHC 3 cut(s) 197, 262, 286
Bsp143I GATC 3 cut(s) 23, 272, 507
BspACI CCGC 2 cut(s) 51, 594
BspCNI CTCAG 3 cut(s) 338, 421, 458
BspDI ATCGAT 2 cut(s) 26, 428
BspFNI CGCG 2 cut(s) 39, 53
BspLI GGNNCC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 2 cut(s) 190, 566
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 3 cut(s) 9, 99, 576
BssMI GATC 3 cut(s) 23, 272, 507
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 3 cut(s) 325, 408, 445
BstDSI CCRYGG 1 cut(s) 9
BstFNI CGCG 2 cut(s) 39, 53
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 3 cut(s) 26, 275, 510
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 3 cut(s) 23, 272, 507
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 11, 86, 593
BstNI CCWGG 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 98
BstSLI GKGCMC 1 cut(s) 197
BstUI CGCG 2 cut(s) 39, 53
BstV1I GCAGC 1 cut(s) 232
Bsu15I ATCGAT 2 cut(s) 26, 428
BsuTUI ATCGAT 2 cut(s) 26, 428
BtgI CCRYGG 1 cut(s) 9
Cac8I GCNNGC 1 cut(s) 478
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 461
ClaI ATCGAT 2 cut(s) 26, 428
CseI GACGC 1 cut(s) 45
Csp6I GTAC 2 cut(s) 183, 618
CspAI ACCGGT 1 cut(s) 461
CviAII CATG 1 cut(s) 421
CviJI RGCY 8 cut(s) 5, 14, 146, 245, 369, 406, 480, 593
CviKI_1 RGCY 8 cut(s) 5, 14, 146, 245, 369, 406, 480, 593
CviQI GTAC 2 cut(s) 183, 618
DdeI CTNAG 3 cut(s) 325, 408, 445
DpnI GATC 3 cut(s) 25, 274, 509
DpnII GATC 3 cut(s) 23, 272, 507
DriI GACNNNNNGTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 41
Eco57I CTGAAG 2 cut(s) 261, 515
Eco88I CYCGRG 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 98
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 1 cut(s) 424
FaiI YATR 7 cut(s) 112, 129, 335, 422, 506, 549, 606
FatI CATG 1 cut(s) 420
FblI GTMKAC 1 cut(s) 34
Fnu4HI GCNGC 3 cut(s) 6, 246, 594
Fsp4HI GCNGC 3 cut(s) 6, 246, 594
GlaI GCGC 1 cut(s) 54
GluI GCNGC 3 cut(s) 6, 246, 594
GsuI CTGGAG 1 cut(s) 124
HapII CCGG 1 cut(s) 462
HgaI GACGC 1 cut(s) 45
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 1 cut(s) 424
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 35
HindII GTYRAC 1 cut(s) 35
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 3 cut(s) 28, 268, 441
HpaII CCGG 1 cut(s) 462
HphI GGTGA 3 cut(s) 380, 508, 565
Hpy166II GTNNAC 4 cut(s) 35, 67, 400, 454
Hpy188I TCNGA 3 cut(s) 23, 123, 411
Hpy188III TCNNGA 4 cut(s) 141, 287, 613, 631
Hpy8I GTNNAC 4 cut(s) 35, 67, 400, 454
Hpy99I CGWCG 4 cut(s) 36, 39, 44, 47
HpyAV CCTTC 2 cut(s) 105, 494
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 3 cut(s) 131, 248, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 86, 593
HpyF3I CTNAG 3 cut(s) 325, 408, 445
HpySE526I ACGT 1 cut(s) 42
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 1 cut(s) 424
HspAI GCGC 1 cut(s) 53
Kzo9I GATC 3 cut(s) 23, 272, 507
Lsp1109I GCAGC 1 cut(s) 232
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 2 cut(s) 466, 580
MalI GATC 3 cut(s) 25, 274, 509
MboI GATC 3 cut(s) 23, 272, 507
MboII GAAGA 1 cut(s) 508
MfeI CAATTG 2 cut(s) 249, 263
MhlI GDGCHC 3 cut(s) 197, 262, 286
MluCI AATT 2 cut(s) 249, 263
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 7 cut(s) 66, 208, 343, 396, 417, 524, 586
MseI TTAA 1 cut(s) 640
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 1 cut(s) 100
MunI CAATTG 2 cut(s) 249, 263
MvaI CCWGG 1 cut(s) 100
MvnI CGCG 2 cut(s) 39, 53
MwoI GCNNNNNNNGC 3 cut(s) 11, 86, 593
NdeII GATC 3 cut(s) 23, 272, 507
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PfeI GAWTC 2 cut(s) 28, 441
PflFI GACNNNGTC 1 cut(s) 44
PinAI ACCGGT 1 cut(s) 461
PkrI GCNGC 3 cut(s) 7, 247, 595
Ple19I CGATCG 1 cut(s) 26
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 98
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 354
PsyI GACNNNGTC 1 cut(s) 44
PvuI CGATCG 1 cut(s) 26
RsaI GTAC 2 cut(s) 184, 619
RsaNI GTAC 2 cut(s) 183, 618
SalI GTCGAC 1 cut(s) 33
SaqAI TTAA 1 cut(s) 640
SatI GCNGC 3 cut(s) 6, 246, 594
Sau3AI GATC 3 cut(s) 23, 272, 507
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 3 cut(s) 197, 262, 286
SgrDI CGTCGACG 1 cut(s) 33
Sse9I AATT 2 cut(s) 249, 263
SsiI CCGC 2 cut(s) 51, 594
StyD4I CCNGG 1 cut(s) 98
TaiI ACGT 1 cut(s) 45
TaqI TCGA 5 cut(s) 26, 34, 271, 302, 428
TasI AATT 2 cut(s) 249, 263
TatI WGTACW 1 cut(s) 617
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 2 cut(s) 28, 441
Tru1I TTAA 1 cut(s) 640
Tru9I TTAA 1 cut(s) 640
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 5, 245
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 2 cut(s) 99, 428
TspGWI ACGGA 2 cut(s) 52, 269
Tth111I GACNNNGTC 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 34
ZraI GACGTC 1 cut(s) 43
ZrmI AGTACT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.