Rh6DG202700
HSP70 Family

succinate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010847
Physical Location & Seq
Reverse (-)
36141354 .. 36144914
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG202700.1

Sequence Viewer

Length: 810 bp
ATGCTGATTGACTTGATATTTGTAGGAAAAACCTTCATGGACCATGATACTGGAAGATCTACAGGACTTGGATTCATTACTTCTAGTGCCATCCATCCCTTGGACGGGCAGGAGCTCCATGGTCGCCAAGTAAGGTTGAATTATTCTACTGATAGGCCTCGAAGTAATTTTGGAAGTTGTTATGGTGGTTACACAGGAGGTGTAGTAGGTTATTATGGTCAACATGGTTCTGGATATGGTTTTGGTGTTGGTTATGGTTTGAGGTGTTATGGAGTTGGAGCAGGTGACAAGTTTTCATCCACTGGTAATAGCAGCAACTATGGTCCTGGTGCAGGTGTTGGTAGTGCAGTCAACTCTACTATTGGTAGTGCTGATAAATCTGGTGTTGGTCAGAGAAACAGCTTTTCCAGTGGAGCTGCTGGAAACACTGGGGTTGGATATGAAATTGCCTACACCCGCAGTGGGGCAAGCACAGCTGATGAACATGGCATCCTACCCAATACTCTTCGTCCATTATCCCCCCATCTTCCTGTTTACAAGCCACAAATCAATTCGACACGTTCAATACTTGACAGAATTGCAGCATCTTACCTAACCGCTATAATTTTGCTGTACTATCTTGTTTGTGTGAAAATGGGTTCTATTTGCTTCACCTTTGAGTATTTCTACAAATTCCTCTTTTATTCAGCAAGCCTTGTCCCCCTCTCCCTTGAGCTTTCTGCTGTAGCAATGGCCTATCATACGTATTATGGGATTGGTCATCTATTGAACGACTTTAAGGGTTTTAGGTTTCTTAGATTTGGAAAGTAG

Protein Analysis

269

Amino Acids

28.82

Weight (kDa)

9.05

Isoelectric Point (pI)

22.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sdh_cyt PF01127 170 - 262 5.3e-11 Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 272, 323
Acc36I ACCTGC 2 cut(s) 272, 323
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 2 cut(s) 457, 597
AcsI RAATTY 1 cut(s) 671
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 6 cut(s) 100, 104, 105, 332, 462, 463
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 3 cut(s) 139, 564, 769
AjnI CCWGG 1 cut(s) 325
AloI GAACNNNNNNTCC 2 cut(s) 474, 506
AluBI AGCT 5 cut(s) 115, 402, 416, 476, 715
AluI AGCT 5 cut(s) 115, 402, 416, 476, 715
Alw21I GWGCWC 1 cut(s) 117
AoxI GGCC 2 cut(s) 155, 732
ApeKI GCWGC 3 cut(s) 312, 416, 581
ApoI RAATTY 1 cut(s) 671
ArsI GACNNNNNNTTYG 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 40, 323
AsuHPI GGTGA 2 cut(s) 296, 643
AvaII GGWCC 2 cut(s) 40, 323
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 324, 403, 593
BccI CCATC 3 cut(s) 98, 102, 531
BciT130I CCWGG 1 cut(s) 327
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 2 cut(s) 60, 723
BfuAI ACCTGC 2 cut(s) 272, 323
BglII AGATCT 1 cut(s) 56
BisI GCNGC 3 cut(s) 313, 417, 582
BlsI GCNGC 3 cut(s) 314, 418, 583
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 2 cut(s) 40, 323
BmgT120I GGNCC 2 cut(s) 40, 323
BmrFI CCNGG 1 cut(s) 327
BmrI ACTGGG 1 cut(s) 438
BmsI GCATC 2 cut(s) 498, 593
BmuI ACTGGG 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 731
BsaAI YACGTR 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 99, 118
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 104, 105, 332, 462, 463
Bse1I ACTGG 4 cut(s) 55, 307, 408, 433
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 99, 118
BseGI GGATG 4 cut(s) 90, 94, 296, 489
BseLI CCNNNNNNNGG 6 cut(s) 100, 104, 105, 332, 462, 463
BseMI GCAATG 1 cut(s) 735
BseNI ACTGG 4 cut(s) 55, 307, 408, 433
BseXI GCAGC 3 cut(s) 324, 403, 593
BsgI GTGCAG 2 cut(s) 351, 366
BshFI GGCC 2 cut(s) 157, 734
BsiHKAI GWGCWC 1 cut(s) 117
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 6 cut(s) 100, 104, 105, 332, 462, 463
BsmFI GGGAC 1 cut(s) 683
BsnI GGCC 2 cut(s) 157, 734
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 1 cut(s) 56
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 2 cut(s) 457, 597
BspANI GGCC 2 cut(s) 157, 734
BspMI ACCTGC 2 cut(s) 272, 323
BsrDI GCAATG 1 cut(s) 735
BsrI ACTGG 4 cut(s) 55, 307, 408, 433
BssECI CCNNGG 2 cut(s) 99, 118
BssMI GATC 1 cut(s) 56
BssT1I CCWWGG 2 cut(s) 99, 118
Bst2UI CCWGG 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 469, 691
BstDEI CTNAG 1 cut(s) 794
BstDSI CCRYGG 1 cut(s) 118
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 4 cut(s) 90, 94, 296, 489
BstKTI GATC 1 cut(s) 59
BstMBI GATC 1 cut(s) 56
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 2 cut(s) 60, 723
BstSNI TACGTA 1 cut(s) 744
BstV1I GCAGC 3 cut(s) 324, 403, 593
BstX2I RGATCY 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 50
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 2 cut(s) 157, 734
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 4 cut(s) 90, 94, 296, 489
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 4 cut(s) 300, 415, 426, 466
BveI ACCTGC 2 cut(s) 272, 323
Cac8I GCNNGC 2 cut(s) 469, 691
Cfr13I GGNCC 2 cut(s) 40, 323
Csp6I GTAC 1 cut(s) 613
CviAII CATG 5 cut(s) 37, 44, 119, 224, 485
CviJI RGCY 9 cut(s) 115, 157, 402, 416, 476, 541, 693, 715, 734
CviKI_1 RGCY 9 cut(s) 115, 157, 402, 416, 476, 541, 693, 715, 734
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 1 cut(s) 794
DpnI GATC 1 cut(s) 58
DpnII GATC 1 cut(s) 56
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 115
Eco105I TACGTA 1 cut(s) 744
Eco130I CCWWGG 2 cut(s) 99, 118
Eco147I AGGCCT 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 40, 323
Eco53kI GAGCTC 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 2 cut(s) 99, 118
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 2 cut(s) 99, 118
FaeI CATG 5 cut(s) 40, 47, 122, 227, 488
FaqI GGGAC 1 cut(s) 683
FatI CATG 5 cut(s) 36, 43, 118, 223, 484
FauI CCCGC 1 cut(s) 464
Fnu4HI GCNGC 3 cut(s) 313, 417, 582
FokI GGATG 4 cut(s) 77, 81, 283, 476
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 3 cut(s) 313, 417, 582
FspBI CTAG 1 cut(s) 84
GluI GCNGC 3 cut(s) 313, 417, 582
HaeIII GGCC 2 cut(s) 157, 734
Hin1II CATG 5 cut(s) 40, 47, 122, 227, 488
HincII GTYRAC 2 cut(s) 221, 352
HindII GTYRAC 2 cut(s) 221, 352
HinfI GANTC 1 cut(s) 72
HphI GGTGA 2 cut(s) 296, 643
Hpy166II GTNNAC 3 cut(s) 221, 352, 535
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 1 cut(s) 231
Hpy8I GTNNAC 3 cut(s) 221, 352, 535
HpyAV CCTTC 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 559, 743
HpyCH4V TGCA 3 cut(s) 332, 347, 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 1 cut(s) 794
HpySE526I ACGT 2 cut(s) 559, 743
Hsp92II CATG 5 cut(s) 40, 47, 122, 227, 488
Kzo9I GATC 1 cut(s) 56
LmnI GCTCC 4 cut(s) 112, 120, 278, 413
Lsp1109I GCAGC 3 cut(s) 324, 403, 593
LweI GCATC 2 cut(s) 498, 593
MaeI CTAG 1 cut(s) 84
MaeII ACGT 2 cut(s) 559, 743
MaeIII GTNAC 2 cut(s) 188, 284
MalI GATC 1 cut(s) 58
MboI GATC 1 cut(s) 56
MboII GAAGA 3 cut(s) 66, 497, 518
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 7 cut(s) 139, 166, 444, 550, 576, 603, 671
MmeI TCCRAC 2 cut(s) 256, 415
MnlI CCTC 5 cut(s) 168, 191, 255, 686, 713
MseI TTAA 1 cut(s) 777
MspA1I CMGCKG 1 cut(s) 476
MspR9I CCNGG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 473
NcoI CCATGG 1 cut(s) 118
NdeII GATC 1 cut(s) 56
NlaIII CATG 5 cut(s) 40, 47, 122, 227, 488
NmuCI GTSAC 1 cut(s) 284
PaqCI CACCTGC 2 cut(s) 272, 323
PceI AGGCCT 1 cut(s) 157
PfeI GAWTC 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 3 cut(s) 314, 418, 583
Ppu21I YACGTR 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspPI GGNCC 2 cut(s) 40, 323
PsuI RGATCY 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 777
SatI GCNGC 3 cut(s) 313, 417, 582
Sau3AI GATC 1 cut(s) 56
Sau96I GGNCC 2 cut(s) 40, 323
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 117
SfaNI GCATC 2 cut(s) 498, 593
SfcI CTRYAG 2 cut(s) 60, 723
SinI GGWCC 2 cut(s) 40, 323
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SnaBI TACGTA 1 cut(s) 744
Sse9I AATT 7 cut(s) 139, 166, 444, 550, 576, 603, 671
SseBI AGGCCT 1 cut(s) 157
SsiI CCGC 2 cut(s) 457, 597
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 117
StuI AGGCCT 1 cut(s) 157
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 2 cut(s) 99, 118
TaiI ACGT 2 cut(s) 562, 746
TaqI TCGA 2 cut(s) 160, 554
TasI AATT 7 cut(s) 139, 166, 444, 550, 576, 603, 671
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 4 cut(s) 307, 415, 433, 466
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 3 cut(s) 312, 416, 581
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 5 cut(s) 25, 64, 285, 456, 495
TspRI CASTG 4 cut(s) 307, 415, 433, 466
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 40, 323
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 1 cut(s) 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.