Rh6CG212300
HSP70 Family

succinate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010847
Physical Location & Seq
Reverse (-)
35280315 .. 35283630
3316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG212300.1

Sequence Viewer

Length: 978 bp
ATGCCTCTCCTAAGTAAAGCTGGGAGTATACTCAGGCAGACTGCAAACAGGCAGATCAAATCTGAGTTATCGATCCATCAGGCAATAAGATGCACATCATCCATGGCAAGCTCAGAAGCCTTAAGAGGTACTTTGTATCAGACGGATCAACAAAGTCTGAGGAAAGCTTTTGCAAAATCTGGTGAAGTTGATGGAAAAACCTTCATGCACCATGATACTGGAAGATCTACAGGACTTGGATTCATTACTTCTAGTGCCATCCATCCCTTGGACGGGCAGGAGCTCCATGGTCGCCAAGTAAGGTTGAATTATTCTACTGATAGGCCTCGAAGTAATTTTGGAAGTTGTTATGGTGGTTACACAGGAGGTGTAGTAGGTTATTATGGTCAACATGGTTCTGGATATGGTTTTGGTGTTGGTTATGGTTTGAGGTGTTATGGAGGTGGAGCAGGTGACAAGTTTTCATCCACTGGTAATAGCAGCAACTATGGTCCTGGTGCAGGTGTTGGTAGTGCAGTCAACTCTACTATTGGTAGTGCTGATAAATCTGGTGTTGGTCAGAGAAACAGCTTTTCCAGTGGAGCTACTGGAAACACTGGGGTTGGATATGAAATTGCCTACACCCGCAGTGGGGCAAGCACAGCTGATGAACATGGCATCCTACCCAATACTCTTCGTCCATTATCCCCCCATCTTCCTGTTTACAAGCCACAAATCAATTCGACACGTTCAATACTTGACAGAATTGCAGCATCTTACCTAACCGCTATAATTTTGCTGTACTATCTTGTTTGTGTGAAAATGGGTTCTATTTGCTTCACCTTTGAGTATTTCTACAAATTCCTCTTTTATTCAGCAAGCCTTGTCCCCCTCTCCCTTGAGCTTTCTGCTGTAGCAATGGCCTATCATACGTATTATGGGATCGGTCATCTATTGAACGACTTTAAGGGTTTTAGGTTTCTTAGATTTGGAAAGTAG

Protein Analysis

325

Amino Acids

34.88

Weight (kDa)

9.44

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sdh_cyt PF01127 226 - 318 7.9e-11 Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 440, 491
Acc36I ACCTGC 2 cut(s) 440, 491
AccB7I CCANNNNNTGG 1 cut(s) 268
AccI GTMKAC 1 cut(s) 28
AciI CCGC 2 cut(s) 625, 765
AclWI GGATC 3 cut(s) 67, 153, 929
AcsI RAATTY 1 cut(s) 839
AfaI GTAC 2 cut(s) 130, 782
AfiI CCNNNNNNNGG 6 cut(s) 268, 272, 273, 500, 630, 631
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 3 cut(s) 307, 732, 937
AjnI CCWGG 1 cut(s) 493
AloI GAACNNNNNNTCC 2 cut(s) 642, 674
AluBI AGCT 8 cut(s) 20, 111, 167, 283, 570, 584, 644, 883
AluI AGCT 8 cut(s) 20, 111, 167, 283, 570, 584, 644, 883
Alw21I GWGCWC 1 cut(s) 285
AlwI GGATC 3 cut(s) 67, 153, 929
AoxI GGCC 2 cut(s) 323, 900
ApeKI GCWGC 2 cut(s) 480, 749
ApoI RAATTY 1 cut(s) 839
AspS9I GGNCC 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 194, 464, 811
AvaII GGWCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 492, 761
BccI CCATC 5 cut(s) 84, 185, 266, 270, 699
BciT130I CCWGG 1 cut(s) 495
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 228, 891
BfrI CTTAAG 1 cut(s) 121
BfuAI ACCTGC 2 cut(s) 440, 491
BglII AGATCT 1 cut(s) 224
BisI GCNGC 2 cut(s) 481, 750
BlsI GCNGC 2 cut(s) 482, 751
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 491
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 3 cut(s) 80, 666, 761
BmuI ACTGGG 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 899
Bsa29I ATCGAT 1 cut(s) 71
BsaAI YACGTR 1 cut(s) 912
BsaBI GATNNNNATC 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 102, 267, 286
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 6 cut(s) 268, 272, 273, 500, 630, 631
Bse1I ACTGG 5 cut(s) 223, 475, 576, 592, 601
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 94
BseBI CCWGG 1 cut(s) 495
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 3 cut(s) 102, 267, 286
BseGI GGATG 5 cut(s) 98, 258, 262, 464, 657
BseJI GATNNNNATC 1 cut(s) 94
BseLI CCNNNNNNNGG 6 cut(s) 268, 272, 273, 500, 630, 631
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 4 cut(s) 46, 54, 126, 149
BseNI ACTGG 5 cut(s) 223, 475, 576, 592, 601
BseXI GCAGC 2 cut(s) 492, 761
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 2 cut(s) 519, 534
BshFI GGCC 2 cut(s) 325, 902
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 285
BslFI GGGAC 1 cut(s) 851
BslI CCNNNNNNNGG 6 cut(s) 268, 272, 273, 500, 630, 631
BsmFI GGGAC 1 cut(s) 851
BsnI GGCC 2 cut(s) 325, 902
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 5 cut(s) 54, 72, 145, 224, 921
Bsp19I CCATGG 2 cut(s) 102, 286
BspACI CCGC 2 cut(s) 625, 765
BspANI GGCC 2 cut(s) 325, 902
BspCNI CTCAG 4 cut(s) 45, 55, 125, 150
BspDI ATCGAT 1 cut(s) 71
BspMI ACCTGC 2 cut(s) 440, 491
BspPI GGATC 3 cut(s) 67, 153, 929
BspTI CTTAAG 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 903
BsrI ACTGG 5 cut(s) 223, 475, 576, 592, 601
BssECI CCNNGG 3 cut(s) 102, 267, 286
BssMI GATC 5 cut(s) 54, 72, 145, 224, 921
BssNAI GTATAC 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 102, 267, 286
Bst1107I GTATAC 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 495
Bst6I CTCTTC 1 cut(s) 678
BstAFI CTTAAG 1 cut(s) 121
BstBAI YACGTR 1 cut(s) 912
BstC8I GCNNGC 3 cut(s) 109, 637, 859
BstDEI CTNAG 6 cut(s) 11, 32, 63, 112, 158, 962
BstDSI CCRYGG 2 cut(s) 102, 286
BstENI CCTNNNNNAGG 1 cut(s) 498
BstF5I GGATG 5 cut(s) 98, 258, 262, 464, 657
BstKTI GATC 5 cut(s) 57, 75, 148, 227, 924
BstMBI GATC 5 cut(s) 54, 72, 145, 224, 921
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstNI CCWGG 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 2 cut(s) 228, 891
BstSNI TACGTA 1 cut(s) 912
BstV1I GCAGC 2 cut(s) 492, 761
BstX2I RGATCY 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 218
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 71
BsuRI GGCC 2 cut(s) 325, 902
BsuTUI ATCGAT 1 cut(s) 71
BtgI CCRYGG 2 cut(s) 102, 286
BtsCI GGATG 5 cut(s) 98, 258, 262, 464, 657
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 4 cut(s) 468, 583, 594, 634
BveI ACCTGC 2 cut(s) 440, 491
Cac8I GCNNGC 3 cut(s) 109, 637, 859
Cfr13I GGNCC 1 cut(s) 491
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 2 cut(s) 129, 781
CviAII CATG 6 cut(s) 103, 205, 212, 287, 392, 653
CviQI GTAC 2 cut(s) 129, 781
DdeI CTNAG 6 cut(s) 11, 32, 63, 112, 158, 962
DpnI GATC 5 cut(s) 56, 74, 147, 226, 923
DpnII GATC 5 cut(s) 54, 72, 145, 224, 921
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 283
Eco105I TACGTA 1 cut(s) 912
Eco130I CCWWGG 3 cut(s) 102, 267, 286
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 493
EcoT14I CCWWGG 3 cut(s) 102, 267, 286
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 3 cut(s) 102, 267, 286
FaeI CATG 6 cut(s) 106, 208, 215, 290, 395, 656
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FaqI GGGAC 1 cut(s) 851
FatI CATG 6 cut(s) 102, 204, 211, 286, 391, 652
FauI CCCGC 1 cut(s) 632
FblI GTMKAC 1 cut(s) 28
Fnu4HI GCNGC 2 cut(s) 481, 750
FokI GGATG 5 cut(s) 85, 245, 249, 451, 644
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 2 cut(s) 481, 750
FspBI CTAG 1 cut(s) 252
GluI GCNGC 2 cut(s) 481, 750
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 325, 902
Hin1II CATG 6 cut(s) 106, 208, 215, 290, 395, 656
HincII GTYRAC 2 cut(s) 389, 520
HindII GTYRAC 2 cut(s) 389, 520
HindIII AAGCTT 1 cut(s) 165
HinfI GANTC 1 cut(s) 240
HphI GGTGA 3 cut(s) 194, 464, 811
Hpy166II GTNNAC 4 cut(s) 29, 389, 520, 703
Hpy188I TCNGA 5 cut(s) 64, 115, 141, 159, 561
Hpy188III TCNNGA 1 cut(s) 399
Hpy8I GTNNAC 4 cut(s) 29, 389, 520, 703
HpyAV CCTTC 1 cut(s) 211
HpyCH4IV ACGT 2 cut(s) 727, 911
HpyCH4V TGCA 7 cut(s) 44, 93, 173, 208, 500, 515, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 6 cut(s) 11, 32, 63, 112, 158, 962
HpySE526I ACGT 2 cut(s) 727, 911
Hsp92II CATG 6 cut(s) 106, 208, 215, 290, 395, 656
Kzo9I GATC 5 cut(s) 54, 72, 145, 224, 921
LmnI GCTCC 4 cut(s) 280, 288, 446, 581
Lsp1109I GCAGC 2 cut(s) 492, 761
LweI GCATC 3 cut(s) 80, 666, 761
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 727, 911
MaeIII GTNAC 2 cut(s) 356, 452
MalI GATC 5 cut(s) 56, 74, 147, 226, 923
MboI GATC 5 cut(s) 54, 72, 145, 224, 921
MboII GAAGA 3 cut(s) 234, 665, 686
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 7 cut(s) 307, 334, 612, 718, 744, 771, 839
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 9 cut(s) 15, 119, 153, 336, 359, 423, 434, 854, 881
MseI TTAA 2 cut(s) 122, 945
MspA1I CMGCKG 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 641
NcoI CCATGG 2 cut(s) 102, 286
NdeII GATC 5 cut(s) 54, 72, 145, 224, 921
NlaIII CATG 6 cut(s) 106, 208, 215, 290, 395, 656
NmuCI GTSAC 1 cut(s) 452
PaqCI CACCTGC 2 cut(s) 440, 491
PceI AGGCCT 1 cut(s) 325
PfeI GAWTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 2 cut(s) 482, 751
Ppu21I YACGTR 1 cut(s) 912
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 1 cut(s) 493
PspPI GGNCC 1 cut(s) 491
PsuI RGATCY 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 130, 782
RsaNI GTAC 2 cut(s) 129, 781
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 2 cut(s) 122, 945
SatI GCNGC 2 cut(s) 481, 750
Sau3AI GATC 5 cut(s) 54, 72, 145, 224, 921
Sau96I GGNCC 1 cut(s) 491
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 285
SfaNI GCATC 3 cut(s) 80, 666, 761
SfcI CTRYAG 2 cut(s) 228, 891
SinI GGWCC 1 cut(s) 491
SmlI CTYRAG 2 cut(s) 121, 878
SmoI CTYRAG 2 cut(s) 121, 878
SnaBI TACGTA 1 cut(s) 912
Sse9I AATT 7 cut(s) 307, 334, 612, 718, 744, 771, 839
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 2 cut(s) 625, 765
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 3 cut(s) 102, 267, 286
TaiI ACGT 2 cut(s) 730, 914
TaqI TCGA 3 cut(s) 71, 328, 722
TaqII GACCGA 1 cut(s) 914
TasI AATT 7 cut(s) 307, 334, 612, 718, 744, 771, 839
TatI WGTACW 1 cut(s) 780
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 2 cut(s) 122, 945
Tru9I TTAA 2 cut(s) 122, 945
TscAI CASTG 4 cut(s) 475, 583, 601, 634
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 2 cut(s) 480, 749
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 5 cut(s) 193, 232, 453, 624, 663
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 4 cut(s) 475, 583, 601, 634
Van91I CCANNNNNTGG 1 cut(s) 268
Vha464I CTTAAG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 491
XagI CCTNNNNNAGG 1 cut(s) 498
XapI RAATTY 1 cut(s) 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 28
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.