Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007989

Physical Location & Seq

Genomic Coordinates Reverse (-)

16126179 .. 16130608

Gene Structure 4430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G33530.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2568 bp

ATGTTTCACGTGGAAGAAGAAAGCAGTGGAGGCGATGGGTCTGAGATTGATGAGGAGTTTGGCGGAGACGATTCAACGACGTCATTGTCGCGATGGGTGTTCGATGAGAAAGATGATTATGAGGTTAATGAAGATTACGACGATGATGGATATGATGAGCATAATCACCCGGAAATGGATTCCGATGAGGAGGATGACAATGTGGAGCAGCGTTTGATTCGCACTAGCCCTGCCGTTGACTCTTTCGATGTAGATGCTCTTGAGATTCCTGGAACTCAGAAAAACGAAATCGAGGACACTGGTATAGGAAAGAAACTCATACTTGCTTTGCAGACACTTGGGGTTGTATTTGGTGATATTGGAACTAGCCCATTGTATACCTTCACTGTCATGTTCAGAAGATCTCCAATTAACGACAAAGAAGATATTATTGGAGCCTTGTCATTGGTTATATACACTTTAATATTGATTCCTCTTGTAAAGTATGTACATTTTGTTCTTTGGGCCAATGACGATGGCGAAGGTGGGACGTTTGCTTTGTATTCGTTGATCTGCCGGCATGCAAATGTTAGCCTTATCCCAAATCAACTTCCATCAGATGCTCGCATATCAGGCTTTGGTTTGAAGGTTCCGTCTCCAGAACTTGAAAGATCATTGATAATTAAAGAAAGACTTGAGGCGTCAATGGCCTTGAAAAAGCTTCTTCTGATTTTAGTTCTTGCCGGCACTGCTATGGTGATTGCTGATGCTGTTGTTACGCCAGCAATGTCAGTAATGTCTGCTATCGGTGGTCTGAAGGTTGGAGTTGGTGTTATAGAACAAGATCAGGTGGTCGTGATATCAGTCAGCTTTCTTGTGATCTTGTTCAGTGTACAAAAATATGGAACCAGCAAATTGGGGCTTGTTTTGGGTCCTGCTTTACTTCTGTGGTTTTTTTGTCTAGCGGGCATTGGAATTTACAACCTCGTAAAATATGACAGCAGCGTTTTTAAAGCGTTCAATCCTGCATATATCTATTTCTTTTTCAAGAGAAACTCTGTAAATGCTTGGTATGCACTTGGGGGTTGCGTTTTATGTGCAACTGGATCGGAGGCCATGTTTGCAGATCTTAGCTATTTCTCTGTTCACTCTATCCAGCTTACTTTTATCCTTCTGGTGTTACCTTGCCTCTTGCTGGGTTATTTGGGTCAAGCCGCATACCTCTCTGAAAACTTCAGCGCTGCCGGGGATGCTTTCTTTTCGTCAGTTCCAAGTTCTTTGTTCTGGCCAGTCTTTCTCATTTCTAATGTTGCTGCTTTAATTGCCAGTCGTGCAATGACAACAGCCACATTTACATGCATCAAACAGTCAATAGCACTAGGCTGTTTCCCACGTCTTAAAATCATTCACACCTCAAAGAAATTCATTGGACAGATTTATATACCGGTTCTTAACTGGTCCCTTCTGGTGGTGTGTCTGATCGTTGTCTGCTCTACCTCAAATATCTTCGCGATTGGAAACGCTTATGGCATCGCAGAGCTGGGAATTATGATGACTACAACAATTTTGGTGACCCTTATCATGCTTCTTATCTGGCAGACGAACATCATAGTTGTGAGCATGTTTGCAATTGTTTCCCTGATAGTCGAACTGGTTTTCTTCTCATCCGTTTGTTCAAGTGTGGCTGATGGAAGTTGGATAATCTTGGTTTTTGCTACAATTATGTTTCTCATAATGTTTGTTTGGAACTACGGGAGTAAACTGAAGTATGAAACTGAAGTCCAGAAAAAGCTACCAATGGACCTACTACGAGAACTGGGCAGTAACCTTGGGACAATTAGAGCACCCGGTATTGGTCTACTTTATAATGAGCTAGCTAAAGGAGTTCCGGCAATATTTGGTCATTTTCTAACCACCCTTCCTGCAATCCATTCCATGGTTATCTTCGTGTGTATAAAGTATGTCCCTGTTCCAAGTGTGCCTCAGACCGAGAGATTTCTTTTCAGACGTGTATGCCCAAGAAGCTATCATTTATTCCGCTGTGTAGCCAGGTATGGATACAAAGATGTGCGGAAGGAAAGTCACCAGGCGTTTGAGCAGATACTGATTGAGAGTCTAGAAAAATTTATACGTAAGGAAGCACAGGAGCGTGCACTTGAGAGTGACGGAGACCATAATGATACAGATTCTGAGGATGATACGACTCTGTCCAGAGTTCTGATAGCACCCAATGGAAGTGTGTATTCACTTGGAGTGCCTCTCTTAGCTGAGCACATGAATTCGTCAAACAAGCGACCCATGGAGAGAAGAAAGGCCAGCATAGATTTTGGGGCAGGTCCTTCATCGGCGTTGGATGTGGAGCAGAGTCTAGAAAAAGAATTGTCGTTTATACACAAAGCGAAAGAGTCAGGGGTGGTGTATCTACTGGGACATGGGGACATAAGGGCTACTAAGGATTCTTGGTTTCTGAAGAAGCTGGTAATAAATTACTTGTATGCTTTCTTGAGGAAGAACTCCAGGAGAGGGATAACAAACCTAAGCGTTCCGCATACACATTTGATGCAAGTTGGAATGACTTATATGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004016 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009975 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0016829 GO:0016849 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

855

Amino Acids

94.74

Weight (kDa)

5.36

Isoelectric Point (pI)

39.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	4 - 366	5.2e-109	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	381 - 625	6.3e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007989)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33530 AT4G33530 AT5G09400
fragaria_vesca	FvH4_2g11940 FvH4_2g11940 FvH4_2g11940 FvH4_2g11940
malus_domestica	MD05G1045000.v1.1 MD10G1051900.v1.1
prunus_persica	Prupe.8G060500_v2.0.a1 Prupe.8G060500_v2.0.a1 Prupe.8G060500_v2.0.a1
pyrus_communis	pycom05g03750 pycom10g03670
rosa_chinensis	RchiOBHm_Chr6g0271831
rosa_laevigata	RLG00000013711
rosa_multiflora	Rmu_sc0002396.1_g000028
rosa_roxburghii	Rroxscaffold_173G00434990 Rroxscaffold_7G00196740
rosa_rugosa	Rorug06G0063500
rosa_samantha	Rh6AG181700 Rh6BG185800 Rh6CG182300
rosa_wichuraiana	Rw6G015650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1845
AasI	GACNNNNNNGTC	1 cut(s)	85
AatII	GACGTC	1 cut(s)	83
Acc36I	ACCTGC	1 cut(s)	2303
AccB7I	CCANNNNNTGG	1 cut(s)	1915
AccI	GTMKAC	2 cut(s)	377, 1837
AccII	CGCG	2 cut(s)	91, 1490
AciI	CCGC	6 cut(s)	63, 944, 1194, 2017, 2050, 2525
AclWI	GGATC	1 cut(s)	1093
AcoI	YGGCCR	1 cut(s)	1265
AcsI	RAATTY	4 cut(s)	954, 1400, 2102, 2257
AcuI	CTGAAG	5 cut(s)	815, 1198, 1763, 1776, 2468
AcvI	CACGTG	1 cut(s)	10
AcyI	GRCGYC	2 cut(s)	80, 680
AfaI	GTAC	2 cut(s)	489, 873
AfeI	AGCGCT	1 cut(s)	1219
AfiI	CCNNNNNNNGG	6 cut(s)	175, 1174, 1447, 1832, 1915, 2502
AflIII	ACRYGT	1 cut(s)	1987
AgeI	ACCGGT	1 cut(s)	1423
AgsI	TTSAA	7 cut(s)	75, 625, 647, 694, 1000, 1027, 1656
AjiI	CACGTC	2 cut(s)	1373, 1988
AjnI	CCWGG	4 cut(s)	268, 2027, 2064, 2495
AjuI	GAANNNNNNNTTGG	2 cut(s)	414, 446
Alw21I	GWGCWC	3 cut(s)	1825, 2134, 2253
Alw26I	GTCTC	3 cut(s)	60, 639, 2142
Alw44I	GTGCAC	1 cut(s)	2130
AlwI	GGATC	1 cut(s)	1093
AlwNI	CAGNNNCTG	2 cut(s)	2083, 2168
Aor51HI	AGCGCT	1 cut(s)	1219
AoxI	GGCC	5 cut(s)	504, 687, 1092, 1265, 2292
ApaLI	GTGCAC	1 cut(s)	2130
ApeKI	GCWGC	4 cut(s)	208, 981, 1220, 1292
ApoI	RAATTY	4 cut(s)	954, 1400, 2102, 2257
ArsI	GACNNNNNNTTYG	2 cut(s)	520, 552
AsiGI	ACCGGT	1 cut(s)	1423
AspLEI	GCGC	1 cut(s)	1220
AspS9I	GGNCC	5 cut(s)	504, 911, 1437, 1780, 2315
AsuC2I	CCSGG	3 cut(s)	170, 1225, 1827
AsuHPI	GGTGA	5 cut(s)	158, 365, 748, 1561, 2054
AsuNHI	GCTAGC	1 cut(s)	1852
AvaII	GGWCC	4 cut(s)	911, 1437, 1780, 2315
BaeGI	GKGCMC	1 cut(s)	2134
BalI	TGGCCA	1 cut(s)	1267
BbrPI	CACGTG	1 cut(s)	10
Bbv12I	GWGCWC	3 cut(s)	1825, 2134, 2253
BbvI	GCAGC	4 cut(s)	220, 993, 1207, 1279
BccI	CCATC	6 cut(s)	29, 87, 140, 509, 601, 1661
BceAI	ACGGC	1 cut(s)	218
BcgI	CGANNNNNNTGC	4 cut(s)	236, 270, 1068, 1102
BciT130I	CCWGG	4 cut(s)	270, 2029, 2066, 2497
BciVI	GTATCC	1 cut(s)	2030
BcnI	CCSGG	3 cut(s)	170, 1225, 1827
BcoDI	GTCTC	3 cut(s)	60, 639, 2142
BfaI	CTAG	7 cut(s)	225, 366, 941, 1358, 1853, 2096, 2348
BfoI	RGCGCY	1 cut(s)	1221
BfuAI	ACCTGC	1 cut(s)	2303
BfuI	GTATCC	1 cut(s)	2030
BglII	AGATCT	2 cut(s)	401, 1105
BisI	GCNGC	5 cut(s)	209, 982, 1194, 1221, 1293
BlpI	GCTNAGC	1 cut(s)	2247
BlsI	GCNGC	5 cut(s)	210, 983, 1195, 1222, 1294
Bme1390I	CCNGG	7 cut(s)	170, 270, 1225, 1827, 2029, 2066, 2497
Bme18I	GGWCC	4 cut(s)	911, 1437, 1780, 2315
BmgBI	CACGTC	2 cut(s)	1373, 1988
BmgT120I	GGNCC	5 cut(s)	504, 911, 1437, 1780, 2315
BmiI	GGNNCC	5 cut(s)	436, 630, 886, 912, 1439
BmrFI	CCNGG	7 cut(s)	170, 270, 1225, 1827, 2029, 2066, 2497
BmrI	ACTGGG	2 cut(s)	1805, 2414
BmsI	GCATC	7 cut(s)	244, 589, 736, 1219, 1347, 1518, 2529
BmtI	GCTAGC	1 cut(s)	1856
BmuI	ACTGGG	2 cut(s)	1805, 2414
BplI	GAGNNNNNCTC	2 cut(s)	2223, 2255
BpmI	CTGGAG	2 cut(s)	621, 2479
Bpu10I	CCTNAGC	1 cut(s)	2516
Bpu1102I	GCTNAGC	1 cut(s)	2247
BpuEI	CTTGAG	4 cut(s)	281, 695, 2156, 2503
BpuMI	CCSGG	3 cut(s)	170, 1225, 1827
BsaAI	YACGTR	2 cut(s)	10, 2111
BsaHI	GRCGYC	2 cut(s)	80, 680
BsaI	GGTCTC	1 cut(s)	2142
BsaJI	CCNNGG	4 cut(s)	1224, 1807, 1914, 2277
BsaWI	WCCGGW	1 cut(s)	1423
BsaXI	ACNNNNNCTCC	4 cut(s)	795, 825, 1082, 1112
Bsc4I	CCNNNNNNNGG	6 cut(s)	175, 1174, 1447, 1832, 1915, 2502
Bse118I	RCCGGY	3 cut(s)	555, 722, 1423
Bse1I	ACTGG	8 cut(s)	304, 1087, 1268, 1305, 1439, 1635, 1800, 2409
Bse3DI	GCAATG	2 cut(s)	771, 1320
BseBI	CCWGG	4 cut(s)	270, 2029, 2066, 2497
BseDI	CCNNGG	4 cut(s)	1224, 1807, 1914, 2277
BseGI	GGATG	5 cut(s)	199, 1234, 1643, 2179, 2338
BseLI	CCNNNNNNNGG	6 cut(s)	175, 1174, 1447, 1832, 1915, 2502
BseMI	GCAATG	2 cut(s)	771, 1320
BseMII	CTCAG	5 cut(s)	33, 290, 1976, 2160, 2238
BseNI	ACTGG	8 cut(s)	304, 1087, 1268, 1305, 1439, 1635, 1800, 2409
BseRI	GAGGAG	2 cut(s)	68, 203
BseSI	GKGCMC	1 cut(s)	2134
BseXI	GCAGC	4 cut(s)	220, 993, 1207, 1279
BseYI	CCCAGC	2 cut(s)	1174, 1519
Bsh1236I	CGCG	2 cut(s)	91, 1490
BshFI	GGCC	5 cut(s)	506, 689, 1094, 1267, 2294
BshTI	ACCGGT	1 cut(s)	1423
BsiHKAI	GWGCWC	3 cut(s)	1825, 2134, 2253
BsiSI	CCGG	7 cut(s)	170, 556, 723, 1224, 1424, 1827, 1868
BslFI	GGGAC	6 cut(s)	541, 1423, 1825, 1928, 2421, 2429
BslI	CCNNNNNNNGG	6 cut(s)	175, 1174, 1447, 1832, 1915, 2502
BsmAI	GTCTC	3 cut(s)	60, 639, 2142
BsmBI	CGTCTC	2 cut(s)	60, 639
BsmFI	GGGAC	6 cut(s)	541, 1423, 1825, 1928, 2421, 2429
BsnI	GGCC	5 cut(s)	506, 689, 1094, 1267, 2294
Bso31I	GGTCTC	1 cut(s)	2142
Bsp1286I	GDGCHC	3 cut(s)	1825, 2134, 2253
Bsp1407I	TGTACA	2 cut(s)	487, 871
Bsp143I	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
Bsp1720I	GCTNAGC	1 cut(s)	2247
Bsp19I	CCATGG	2 cut(s)	1914, 2277
Bsp68I	TCGCGA	2 cut(s)	91, 1490
BspACI	CCGC	6 cut(s)	63, 944, 1194, 2017, 2050, 2525
BspANI	GGCC	5 cut(s)	506, 689, 1094, 1267, 2294
BspCNI	CTCAG	5 cut(s)	34, 289, 1975, 2161, 2239
BspFNI	CGCG	2 cut(s)	91, 1490
BspLI	GGNNCC	5 cut(s)	436, 630, 886, 912, 1439
BspMI	ACCTGC	1 cut(s)	2303
BspOI	GCTAGC	1 cut(s)	1856
BspPI	GGATC	1 cut(s)	1093
BspTNI	GGTCTC	1 cut(s)	2142
BsrDI	GCAATG	2 cut(s)	771, 1320
BsrFI	RCCGGY	3 cut(s)	555, 722, 1423
BsrGI	TGTACA	2 cut(s)	487, 871
BsrI	ACTGG	8 cut(s)	304, 1087, 1268, 1305, 1439, 1635, 1800, 2409
BssAI	RCCGGY	3 cut(s)	555, 722, 1423
BssECI	CCNNGG	4 cut(s)	1224, 1807, 1914, 2277
BssMI	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
BssNAI	GTATAC	1 cut(s)	378
BssNI	GRCGYC	2 cut(s)	80, 680
BssT1I	CCWWGG	3 cut(s)	1807, 1914, 2277
Bst1107I	GTATAC	1 cut(s)	378
Bst2UI	CCWGG	4 cut(s)	270, 2029, 2066, 2497
Bst4CI	ACNGT	2 cut(s)	388, 1347
BstACI	GRCGYC	2 cut(s)	80, 680
BstAUI	TGTACA	2 cut(s)	487, 871
BstBAI	YACGTR	2 cut(s)	10, 2111
BstC8I	GCNNGC	9 cut(s)	557, 561, 604, 724, 762, 946, 1854, 2130, 2296
BstDEI	CTNAG	9 cut(s)	42, 276, 1109, 1962, 2169, 2242, 2247, 2430, 2516
BstDSI	CCRYGG	2 cut(s)	1914, 2277
BstEII	GGTNACC	1 cut(s)	1549
BstF5I	GGATG	5 cut(s)	199, 1234, 1643, 2179, 2338
BstFNI	CGCG	2 cut(s)	91, 1490
BstH2I	RGCGCY	1 cut(s)	1221
BstHHI	GCGC	1 cut(s)	1220
BstKTI	GATC	8 cut(s)	404, 552, 653, 826, 861, 1088, 1108, 1461
BstMAI	GTCTC	3 cut(s)	60, 639, 2142
BstMBI	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
BstNI	CCWGG	4 cut(s)	270, 2029, 2066, 2497
BstNSI	RCATGY	3 cut(s)	563, 1338, 1603
BstPI	GGTNACC	1 cut(s)	1549
BstSCI	CCNGG	7 cut(s)	168, 268, 1223, 1825, 2027, 2064, 2495
BstSLI	GKGCMC	1 cut(s)	2134
BstSNI	TACGTA	1 cut(s)	2111
BstUI	CGCG	2 cut(s)	91, 1490
BstV1I	GCAGC	4 cut(s)	220, 993, 1207, 1279
BstX2I	RGATCY	2 cut(s)	401, 1105
BstXI	CCANNNNNNTGG	1 cut(s)	895
BstYI	RGATCY	2 cut(s)	401, 1105
BstZ17I	GTATAC	1 cut(s)	378
BsuI	GTATCC	1 cut(s)	2030
BsuRI	GGCC	5 cut(s)	506, 689, 1094, 1267, 2294
BtgI	CCRYGG	2 cut(s)	1914, 2277
BtgZI	GCGATG	3 cut(s)	48, 106, 1495
BtrI	CACGTC	2 cut(s)	1373, 1988
BtsCI	GGATG	5 cut(s)	199, 1234, 1643, 2179, 2338
BtsI	GCAGTG	2 cut(s)	31, 726
BtsIMutI	CAGTG	5 cut(s)	31, 297, 384, 726, 874
BtuMI	TCGCGA	2 cut(s)	91, 1490
BveI	ACCTGC	1 cut(s)	2303
Cac8I	GCNNGC	9 cut(s)	557, 561, 604, 724, 762, 946, 1854, 2130, 2296
CaiI	CAGNNNCTG	2 cut(s)	2083, 2168
CfoI	GCGC	1 cut(s)	1220
Cfr10I	RCCGGY	3 cut(s)	555, 722, 1423
Cfr13I	GGNCC	5 cut(s)	504, 911, 1437, 1780, 2315
CseI	GACGC	1 cut(s)	669
Csp6I	GTAC	2 cut(s)	488, 872
CspAI	ACCGGT	1 cut(s)	1423
CviQI	GTAC	2 cut(s)	488, 872
DdeI	CTNAG	9 cut(s)	42, 276, 1109, 1962, 2169, 2242, 2247, 2430, 2516
DpnI	GATC	8 cut(s)	403, 551, 652, 825, 860, 1087, 1107, 1460
DpnII	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
DraI	TTTAAA	1 cut(s)	991
DrdI	GACNNNNNNGTC	1 cut(s)	85
DseDI	GACNNNNNNGTC	1 cut(s)	85
EaeI	YGGCCR	1 cut(s)	1265
EciI	GGCGGA	1 cut(s)	78
Eco105I	TACGTA	1 cut(s)	2111
Eco130I	CCWWGG	3 cut(s)	1807, 1914, 2277
Eco31I	GGTCTC	1 cut(s)	2142
Eco32I	GATATC	1 cut(s)	840
Eco47I	GGWCC	4 cut(s)	911, 1437, 1780, 2315
Eco47III	AGCGCT	1 cut(s)	1219
Eco57I	CTGAAG	5 cut(s)	815, 1198, 1763, 1776, 2468
Eco72I	CACGTG	1 cut(s)	10
Eco91I	GGTNACC	1 cut(s)	1549
EcoO109I	RGGNCCY	2 cut(s)	911, 2315
EcoO65I	GGTNACC	1 cut(s)	1549
EcoRI	GAATTC	1 cut(s)	2257
EcoRII	CCWGG	4 cut(s)	268, 2027, 2064, 2495
EcoRV	GATATC	1 cut(s)	840
EcoT14I	CCWWGG	3 cut(s)	1807, 1914, 2277
EcoT22I	ATGCAT	1 cut(s)	1340
ErhI	CCWWGG	3 cut(s)	1807, 1914, 2277
Esp3I	CGTCTC	2 cut(s)	60, 639
FalI	AAGNNNNNCTT	2 cut(s)	657, 689
FaqI	GGGAC	6 cut(s)	541, 1423, 1825, 1928, 2421, 2429
FauI	CCCGC	1 cut(s)	937
FblI	GTMKAC	2 cut(s)	377, 1837
Fnu4HI	GCNGC	5 cut(s)	209, 982, 1194, 1221, 1293
FokI	GGATG	5 cut(s)	206, 1241, 1630, 2186, 2345
Fsp4HI	GCNGC	5 cut(s)	209, 982, 1194, 1221, 1293
FspBI	CTAG	7 cut(s)	225, 366, 941, 1358, 1853, 2096, 2348
GlaI	GCGC	1 cut(s)	1219
GluI	GCNGC	5 cut(s)	209, 982, 1194, 1221, 1293
GsaI	CCCAGC	2 cut(s)	1178, 1523
GsuI	CTGGAG	2 cut(s)	621, 2479
HaeII	RGCGCY	1 cut(s)	1221
HaeIII	GGCC	5 cut(s)	506, 689, 1094, 1267, 2294
HapII	CCGG	7 cut(s)	170, 556, 723, 1224, 1424, 1827, 1868
HgaI	GACGC	1 cut(s)	669
HhaI	GCGC	1 cut(s)	1220
Hin1I	GRCGYC	2 cut(s)	80, 680
Hin6I	GCGC	1 cut(s)	1218
HinP1I	GCGC	1 cut(s)	1218
HincII	GTYRAC	1 cut(s)	238
HindII	GTYRAC	1 cut(s)	238
HindIII	AAGCTT	1 cut(s)	698
HpaII	CCGG	7 cut(s)	170, 556, 723, 1224, 1424, 1827, 1868
HphI	GGTGA	5 cut(s)	158, 365, 748, 1561, 2054
Hpy166II	GTNNAC	7 cut(s)	238, 378, 872, 1126, 1739, 1838, 2132
Hpy8I	GTNNAC	7 cut(s)	238, 378, 872, 1126, 1739, 1838, 2132
Hpy99I	CGWCG	2 cut(s)	82, 143
HpyAV	CCTTC	9 cut(s)	391, 515, 619, 790, 1160, 1451, 1907, 2047, 2328
HpyCH4III	ACNGT	2 cut(s)	388, 1347
HpyCH4IV	ACGT	6 cut(s)	9, 80, 530, 1372, 1987, 2110
HpyF3I	CTNAG	9 cut(s)	42, 276, 1109, 1962, 2169, 2242, 2247, 2430, 2516
HpySE526I	ACGT	6 cut(s)	9, 80, 530, 1372, 1987, 2110
Hsp92I	GRCGYC	2 cut(s)	80, 680
HspAI	GCGC	1 cut(s)	1218
KroI	GCCGGC	2 cut(s)	555, 722
KroNI	GCCGGC	2 cut(s)	557, 724
Kzo9I	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
LmnI	GCTCC	4 cut(s)	205, 434, 2125, 2338
Lsp1109I	GCAGC	4 cut(s)	220, 993, 1207, 1279
LweI	GCATC	7 cut(s)	244, 589, 736, 1219, 1347, 1518, 2529
MaeI	CTAG	7 cut(s)	225, 366, 941, 1358, 1853, 2096, 2348
MaeII	ACGT	6 cut(s)	9, 80, 530, 1372, 1987, 2110
MaeIII	GTNAC	6 cut(s)	754, 1158, 1549, 1802, 2060, 2141
MalI	GATC	8 cut(s)	403, 551, 652, 825, 860, 1087, 1107, 1460
MboI	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
MfeI	CAATTG	1 cut(s)	1608
MflI	RGATCY	2 cut(s)	401, 1105
MhlI	GDGCHC	3 cut(s)	1825, 2134, 2253
MlsI	TGGCCA	1 cut(s)	1267
MluNI	TGGCCA	1 cut(s)	1267
MlyI	GAGTC	5 cut(s)	233, 2101, 2176, 2353, 2393
MmeI	TCCRAC	4 cut(s)	781, 1655, 2310, 2527
Mox20I	TGGCCA	1 cut(s)	1267
Mph1103I	ATGCAT	1 cut(s)	1340
MroNI	GCCGGC	2 cut(s)	555, 722
MscI	TGGCCA	1 cut(s)	1267
MseI	TTAA	8 cut(s)	126, 411, 461, 663, 990, 1298, 1377, 1431
MslI	CAYNNNNRTG	5 cut(s)	389, 564, 731, 1333, 1592
Msp20I	TGGCCA	1 cut(s)	1267
MspA1I	CMGCKG	1 cut(s)	2019
MspI	CCGG	7 cut(s)	170, 556, 723, 1224, 1424, 1827, 1868
MspR9I	CCNGG	7 cut(s)	170, 270, 1225, 1827, 2029, 2066, 2497
MunI	CAATTG	1 cut(s)	1608
MvaI	CCWGG	4 cut(s)	270, 2029, 2066, 2497
MvnI	CGCG	2 cut(s)	91, 1490
NaeI	GCCGGC	2 cut(s)	557, 724
NciI	CCSGG	3 cut(s)	170, 1225, 1827
NcoI	CCATGG	2 cut(s)	1914, 2277
NdeII	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
NgoMIV	GCCGGC	2 cut(s)	555, 722
NheI	GCTAGC	1 cut(s)	1852
NlaIV	GGNNCC	5 cut(s)	436, 630, 886, 912, 1439
NmuCI	GTSAC	3 cut(s)	1549, 2060, 2141
NruI	TCGCGA	2 cut(s)	91, 1490
NsiI	ATGCAT	1 cut(s)	1340
NspI	RCATGY	3 cut(s)	563, 1338, 1603
PaeI	GCATGC	1 cut(s)	563
PdiI	GCCGGC	2 cut(s)	557, 724
PfeI	GAWTC	7 cut(s)	71, 179, 217, 265, 469, 2165, 2435
PflFI	GACNNNGTC	1 cut(s)	2185
PflMI	CCANNNNNTGG	1 cut(s)	1915
PfoI	TCCNGGA	2 cut(s)	268, 2495
PinAI	ACCGGT	1 cut(s)	1423
PkrI	GCNGC	5 cut(s)	210, 983, 1195, 1222, 1294
PleI	GAGTC	5 cut(s)	233, 2100, 2176, 2352, 2392
PmaCI	CACGTG	1 cut(s)	10
PmlI	CACGTG	1 cut(s)	10
PpsI	GAGTC	5 cut(s)	233, 2100, 2176, 2352, 2392
Ppu21I	YACGTR	2 cut(s)	10, 2111
PpuMI	RGGWCCY	2 cut(s)	911, 2315
PsiI	TTATAA	1 cut(s)	1845
Psp5II	RGGWCCY	2 cut(s)	911, 2315
Psp6I	CCWGG	4 cut(s)	268, 2027, 2064, 2495
PspCI	CACGTG	1 cut(s)	10
PspEI	GGTNACC	1 cut(s)	1549
PspFI	CCCAGC	2 cut(s)	1174, 1519
PspGI	CCWGG	4 cut(s)	268, 2027, 2064, 2495
PspN4I	GGNNCC	5 cut(s)	436, 630, 886, 912, 1439
PspPI	GGNCC	5 cut(s)	504, 911, 1437, 1780, 2315
PspPPI	RGGWCCY	2 cut(s)	911, 2315
PsrI	GAACNNNNNNTAC	2 cut(s)	480, 512
PstNI	CAGNNNCTG	2 cut(s)	2083, 2168
PsuI	RGATCY	2 cut(s)	401, 1105
PsyI	GACNNNGTC	1 cut(s)	2185
RruI	TCGCGA	2 cut(s)	91, 1490
RsaI	GTAC	2 cut(s)	489, 873
RsaNI	GTAC	2 cut(s)	488, 872
RseI	CAYNNNNRTG	5 cut(s)	389, 564, 731, 1333, 1592
SaqAI	TTAA	8 cut(s)	126, 411, 461, 663, 990, 1298, 1377, 1431
SatI	GCNGC	5 cut(s)	209, 982, 1194, 1221, 1293
Sau3AI	GATC	8 cut(s)	401, 549, 650, 823, 858, 1085, 1105, 1458
Sau96I	GGNCC	5 cut(s)	504, 911, 1437, 1780, 2315
SchI	GAGTC	5 cut(s)	233, 2101, 2176, 2353, 2393
ScrFI	CCNGG	7 cut(s)	170, 270, 1225, 1827, 2029, 2066, 2497
SduI	GDGCHC	3 cut(s)	1825, 2134, 2253
SfaNI	GCATC	7 cut(s)	244, 589, 736, 1219, 1347, 1518, 2529
SinI	GGWCC	4 cut(s)	911, 1437, 1780, 2315
SmiMI	CAYNNNNRTG	5 cut(s)	389, 564, 731, 1333, 1592
SmlI	CTYRAG	4 cut(s)	260, 674, 2135, 2482
SmoI	CTYRAG	4 cut(s)	260, 674, 2135, 2482
SnaBI	TACGTA	1 cut(s)	2111
SphI	GCATGC	1 cut(s)	563
SsiI	CCGC	6 cut(s)	63, 944, 1194, 2017, 2050, 2525
SspI	AATATT	2 cut(s)	465, 1875
SspMI	CTAG	7 cut(s)	225, 366, 941, 1358, 1853, 2096, 2348
StyD4I	CCNGG	7 cut(s)	168, 268, 1223, 1825, 2027, 2064, 2495
StyI	CCWWGG	3 cut(s)	1807, 1914, 2277
TaaI	ACNGT	2 cut(s)	388, 1347
TaiI	ACGT	6 cut(s)	12, 83, 533, 1375, 1990, 2113
TaqI	TCGA	4 cut(s)	102, 246, 291, 1626
TaqII	GACCGA	1 cut(s)	1982
TatI	WGTACW	2 cut(s)	487, 871
TauI	GCSGC	1 cut(s)	1196
TfiI	GAWTC	7 cut(s)	71, 179, 217, 265, 469, 2165, 2435
Tru1I	TTAA	8 cut(s)	126, 411, 461, 663, 990, 1298, 1377, 1431
Tru9I	TTAA	8 cut(s)	126, 411, 461, 663, 990, 1298, 1377, 1431
TscAI	CASTG	5 cut(s)	31, 304, 391, 733, 874
TseFI	GTSAC	3 cut(s)	1549, 2060, 2141
TseI	GCWGC	4 cut(s)	208, 981, 1220, 1292
Tsp45I	GTSAC	3 cut(s)	1549, 2060, 2141
TspDTI	ATGAA	5 cut(s)	144, 1393, 1764, 2270, 2310
TspGWI	ACGGA	3 cut(s)	621, 1636, 2160
TspRI	CASTG	5 cut(s)	31, 304, 391, 733, 874
Tth111I	GACNNNGTC	1 cut(s)	2185
Van91I	CCANNNNNTGG	1 cut(s)	1915
VneI	GTGCAC	1 cut(s)	2130
VpaK11BI	GGWCC	4 cut(s)	911, 1437, 1780, 2315
XapI	RAATTY	4 cut(s)	954, 1400, 2102, 2257
XbaI	TCTAGA	2 cut(s)	2095, 2347
XceI	RCATGY	3 cut(s)	563, 1338, 1603
XmiI	GTMKAC	2 cut(s)	377, 1837
XspI	CTAG	7 cut(s)	225, 366, 941, 1358, 1853, 2096, 2348
ZraI	GACGTC	1 cut(s)	81
Zsp2I	ATGCAT	1 cut(s)	1340

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.