Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007989

Physical Location & Seq

Genomic Coordinates Reverse (-)

6881478 .. 6891974

Gene Structure 10497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1051900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGGAGGAGGAGGAAGGTCCCGACAGGGGCGAGATCAATGGCGGACTGGCGTCCATGGACTCCATCGAGTCGCGGTGGGTCTTTCAGGACGAGGACGACTCCGAGGATGCGGATACGGAGGAAGATGCGATGCATCGGACGGTTCTCGATTCGGAGGATGATGAAGAGGAGGACGATAATGCCGAGCAGCGCTTGATTCGCACTGGCCCTCGCGTCGATTCCTTTGACGTCGAAGCGCTCGAGGTCCCCGGCGCTCACAGAACCGAATACGAGGATTTCAGTCTTGGAAGGAAAATAATAATTGCTTTTCAGACCCTTGGGGTTGTCTTTGGTGATGTTGGAACAAGTCCATTATATACGTTCAGTGTCATGTTTAGCAAAGCACCTATTAATGGAAATGAGGATGTTATTGGAGCAATGTCGCTGGTCCTATACACGTTAATCTTGATCCCGTTGCTTAAGTATGTGCTGGTGGTTCTTTGGGCTAATGATGATGGTGAAGGTGGTACATTTGCTTTGTATTCATTGATCTGTCGACATGCTAAGGTCAGTCTTCTTCCCAATCAGCTTCCGTCAGATGCCCGGATATCGAGCTTCAGGCTAAAGGTGCCATCTCCTGAACTTGAAAGATCTTTAAAAATCAAGGAAAGGCTTGAGGCTTCACTGACTCTGAAAAAGCTTCTTCTGATGTTAGTGCTTGCTGGTACTGCTATGGTGATAGCTGATGGGGTTGTTACACCAGCAATGTCAGTAGTATCAGCTGTTGGTGGTCTAAAGGTTGGAGTAGAGGCAATTGAGCAAGATGAAGTGGTGATGATTTCGGTTACCTTTCTTGTTATTTTATTCAGTGTACAGAAATTTGGGACAAGTAAAGTTGGACTGGCTGTAGGCCCTGCCTTATTCATATGGTTTTGTTCCCTTGGTGGGATTGGGATTTACAACCTTGTTAAATATGACAGCAGTGTCTTAAAGGCATTCAATCCTGTTCACATCTATTATTTCTTCGAGAGAAACTCAACTAAGGCTTGGTATTCTCTTGGGGGTTGTCTTTTATGCGCAACAGGTTCTGAGGCAATGTTTGCGGATCTTTGCTATTTTTCTGTACGATCAGTCCAGCTTACATTCGTTTTTCTTGTTTTGCCCTGCCTTATGTTGGGTTATTTGGGTCAAGCCGCCTACCTTATGGAGAACCAAGATGGGGCTGAGCTAGCTTTCTTTTCTTCAATCCCAAGTGGTGTTTTCTGGCCTGTGTTGCTCATTGCTAATGTTGCAGCATTGATTGCTAGTCGTGCAATGACAACGGCCACTTTTTCATGCATAAAACAATCAATGGCACTTGGTTGTTTCCCTCGCCTTAAAATCATACATACCTCCCGGAAGTTCATGGGGCAAATTTATATCCCTGTCGTAAATTGGTTTCTGCTGGTTGTTTCCCTAGTGGCTATCTGCACTATTTCAAACATTGATGAGATTGGAAATGCATATGGCATTGCTGAGCTAGGAGTGATGATGATGACGACCATTTTAGTAACAATCGTTATGCTTCTTATATGGCAGATAAACATAATCATCGTGCTGAGTTTCTTAATCATTTTCCTGGGGTTGGAGTTGACCTTTTTCTCATCGGTTTTGTGTAGTGTGGGAGATGGAAGTTGGATTATTTTGGTCTTTGCCATAATTATGTTTGTTATAATGTCTATCTGGAACTATGGAAGCAAGCTTAAGTATGAAACCGAAGTCAAGCAAAAACTTTCAATGGATCTGATGCGAGAATTGGGTTGCAATCTTGGGACAATCAGAGCTCCAGGCATTGGTTTGCTTTACAATGAGCTGGTGAAGGGAATACCAGCAATCTTTGGCCATTTTCTGACCACACTTCCTGCAATCCATTCGATGATCATATTTGTTTGTATAAAGTATGTTCCAGTTCCTGTAGTGCCTCAAAGTGAAAGGTTCCTTTTCCGGCGTGTCTGTCCGAAAAACTACCACATATTTCGTTGTATTGCCAGGTATGGCTATAAGGATGTTCGTAAAGAAAATCACCAAACATTTGAACAGCTACTAATTGAGAGCCTTGAGAAATTCATACGTAGGGAAGCTCAGGAGCGGTCACTGGAGAGCGATGGGGATGATGGTGATACAGACTCAGAAGATGAGACCTCTGTCTCGAGGGTTCTTATAGCCCCCAACGGAAGTGTTTACTCACTTGGTGTTCCTCTTCTGGCTGAGTACAAGGAGACGAGCCGGCAGCCTGTATCAGAAGCAAGCACATCAGAGGTGAAGCCAGTGCTTCCAGAAGATCCAACAGTTTTTGACTCTGAGCAGAGTATGGATAGGGAACTATCGTTTATACGAAAGGCCAAAGAATCGGGGGTTGTTTATCTTCTTGGTCACGGGGATATTAGGGCCAGGAAAGATTCATGGTTTATCAAGAAGTTAATCATCAATTACTTTTATGCTTTCTTGAGGAAGAACTGCAGGAGGGGCACTGCGAATTTGAGTGTGCCCCATTCGCATCTAATGCAGGTTGGGATGACATATATGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004016 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009975 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0016829 GO:0016849 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

850

Amino Acids

94.54

Weight (kDa)

5.11

Isoelectric Point (pI)

41.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	102 - 587	2.8e-145	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	602 - 847	3.4e-24	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007989)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33530 AT4G33530 AT5G09400
fragaria_vesca	FvH4_2g11940 FvH4_2g11940 FvH4_2g11940 FvH4_2g11940
malus_domestica	MD05G1045000.v1.1 MD10G1051900.v1.1
prunus_persica	Prupe.8G060500_v2.0.a1 Prupe.8G060500_v2.0.a1 Prupe.8G060500_v2.0.a1
pyrus_communis	pycom05g03750 pycom10g03670
rosa_chinensis	RchiOBHm_Chr6g0271831
rosa_laevigata	RLG00000013711
rosa_multiflora	Rmu_sc0002396.1_g000028
rosa_roxburghii	Rroxscaffold_173G00434990 Rroxscaffold_7G00196740
rosa_rugosa	Rorug06G0063500
rosa_samantha	Rh6AG181700 Rh6BG185800 Rh6CG182300
rosa_wichuraiana	Rw6G015650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1691
AasI	GACNNNNNNGTC	1 cut(s)	962
AatII	GACGTC	1 cut(s)	231
Acc16I	TGCGCA	1 cut(s)	1057
Acc36I	ACCTGC	1 cut(s)	2515
AccB1I	GGYRCC	1 cut(s)	607
AccB7I	CCANNNNNTGG	1 cut(s)	1811
AccBSI	CCGCTC	1 cut(s)	2107
AccI	GTMKAC	1 cut(s)	535
AccII	CGCG	2 cut(s)	73, 213
AciI	CCGC	6 cut(s)	42, 73, 110, 1082, 1173, 2107
AclWI	GGATC	4 cut(s)	442, 1092, 1767, 2294
AcoI	YGGCCR	2 cut(s)	1302, 1858
AcsI	RAATTY	4 cut(s)	857, 1392, 2081, 2494
AcuI	CTGAAG	1 cut(s)	580
AcyI	GRCGYC	2 cut(s)	50, 228
AdeI	CACNNNGTG	1 cut(s)	2210
AfaI	GTAC	5 cut(s)	508, 706, 852, 1104, 2231
AfeI	AGCGCT	2 cut(s)	191, 237
AfiI	CCNNNNNNNGG	7 cut(s)	26, 392, 924, 1153, 1198, 1603, 1811
AflII	CTTAAG	2 cut(s)	458, 1721
AflIII	ACRYGT	1 cut(s)	435
AgsI	TTSAA	6 cut(s)	626, 979, 1224, 1458, 1755, 2054
AjnI	CCWGG	4 cut(s)	1596, 1804, 2006, 2408
Alw21I	GWGCWC	1 cut(s)	1804
Alw26I	GTCTC	3 cut(s)	2150, 2170, 2231
AlwI	GGATC	4 cut(s)	442, 1092, 1767, 2294
AlwNI	CAGNNNCTG	2 cut(s)	1067, 1931
Ama87I	CYCGRG	2 cut(s)	239, 2167
Aor51HI	AGCGCT	2 cut(s)	191, 237
AoxI	GGCC	7 cut(s)	205, 889, 1244, 1302, 1858, 2358, 2406
ApeKI	GCWGC	3 cut(s)	187, 1271, 2248
ApoI	RAATTY	4 cut(s)	857, 1392, 2081, 2494
AseI	ATTAAT	1 cut(s)	390
AspLEI	GCGC	4 cut(s)	192, 238, 254, 1058
AspS9I	GGNCC	6 cut(s)	17, 206, 244, 427, 890, 2406
AsuC2I	CCSGG	3 cut(s)	249, 583, 1375
AsuHPI	GGTGA	8 cut(s)	344, 509, 727, 823, 1846, 2033, 2147, 2290
AsuNHI	GCTAGC	1 cut(s)	1207
AvaI	CYCGRG	2 cut(s)	239, 2167
AvaII	GGWCC	3 cut(s)	17, 244, 427
BaeGI	GKGCMC	2 cut(s)	2489, 2508
BalI	TGGCCA	1 cut(s)	1860
BanI	GGYRCC	1 cut(s)	607
BanII	GRGCYC	1 cut(s)	1804
BbsI	GAAGAC	1 cut(s)	545
Bbv12I	GWGCWC	1 cut(s)	1804
BbvI	GCAGC	3 cut(s)	199, 1283, 2260
BccI	CCATC	8 cut(s)	71, 488, 619, 719, 1190, 1640, 2117, 2126
BceAI	ACGGC	1 cut(s)	1317
BcgI	CGANNNNNNTGC	2 cut(s)	1872, 1906
BciT130I	CCWGG	4 cut(s)	1598, 1806, 2008, 2410
BciVI	GTATCC	1 cut(s)	106
BclI	TGATCA	1 cut(s)	1896
BcnI	CCSGG	3 cut(s)	249, 583, 1375
BcoDI	GTCTC	3 cut(s)	2150, 2170, 2231
BfaI	CTAG	4 cut(s)	1208, 1284, 1436, 1499
BfmI	CTRYAG	3 cut(s)	885, 1932, 2476
BfoI	RGCGCY	3 cut(s)	193, 239, 255
BfrI	CTTAAG	2 cut(s)	458, 1721
BfuAI	ACCTGC	1 cut(s)	2515
BfuI	GTATCC	1 cut(s)	106
BglII	AGATCT	1 cut(s)	629
BisI	GCNGC	4 cut(s)	188, 1173, 1272, 2249
BlpI	GCTNAGC	2 cut(s)	1203, 1494
BlsI	GCNGC	4 cut(s)	189, 1174, 1273, 2250
Bme1390I	CCNGG	7 cut(s)	249, 583, 1375, 1598, 1806, 2008, 2410
Bme18I	GGWCC	3 cut(s)	17, 244, 427
BmeT110I	CYCGRG	2 cut(s)	239, 2167
BmgT120I	GGNCC	6 cut(s)	17, 206, 244, 427, 890, 2406
BmiI	GGNNCC	4 cut(s)	19, 246, 609, 1955
BmrFI	CCNGG	7 cut(s)	249, 583, 1375, 1598, 1806, 2008, 2410
BmsI	GCATC	7 cut(s)	97, 115, 120, 142, 568, 1755, 2524
BmtI	GCTAGC	1 cut(s)	1211
BoxI	GACNNNNGTC	2 cut(s)	49, 2162
BpiI	GAAGAC	1 cut(s)	545
BplI	GAGNNNNNCTC	4 cut(s)	83, 115, 998, 1030
BpmI	CTGGAG	2 cut(s)	1788, 2135
Bpu10I	CCTNAGC	2 cut(s)	543, 2100
Bpu1102I	GCTNAGC	2 cut(s)	1203, 1494
BpuEI	CTTGAG	3 cut(s)	674, 2096, 2485
BpuMI	CCSGG	3 cut(s)	249, 583, 1375
BsaAI	YACGTR	1 cut(s)	2090
BsaHI	GRCGYC	2 cut(s)	50, 228
BsaI	GGTCTC	1 cut(s)	2150
BsaJI	CCNNGG	6 cut(s)	54, 102, 247, 316, 919, 1597
Bsc4I	CCNNNNNNNGG	7 cut(s)	26, 392, 924, 1153, 1198, 1603, 1811
Bse118I	RCCGGY	1 cut(s)	2244
Bse1I	ACTGG	6 cut(s)	51, 208, 885, 1925, 2118, 2285
Bse3DI	GCAATG	6 cut(s)	423, 750, 1080, 1257, 1299, 1488
BseBI	CCWGG	4 cut(s)	1598, 1806, 2008, 2410
BseDI	CCNNGG	6 cut(s)	54, 102, 247, 316, 919, 1597
BseGI	GGATG	6 cut(s)	112, 163, 409, 2029, 2134, 2538
BseLI	CCNNNNNNNGG	7 cut(s)	26, 392, 924, 1153, 1198, 1603, 1811
BseMI	GCAATG	6 cut(s)	423, 750, 1080, 1257, 1299, 1488
BseMII	CTCAG	8 cut(s)	1059, 1194, 1485, 1568, 2114, 2160, 2217, 2310
BseNI	ACTGG	6 cut(s)	51, 208, 885, 1925, 2118, 2285
BseRI	GAGGAG	3 cut(s)	20, 23, 182
BseSI	GKGCMC	2 cut(s)	2489, 2508
BseXI	GCAGC	3 cut(s)	199, 1283, 2260
BsgI	GTGCAG	1 cut(s)	1432
Bsh1236I	CGCG	2 cut(s)	73, 213
BshFI	GGCC	7 cut(s)	207, 891, 1246, 1304, 1860, 2360, 2408
BshNI	GGYRCC	1 cut(s)	607
BsiHKAI	GWGCWC	1 cut(s)	1804
BsiHKCI	CYCGRG	2 cut(s)	239, 2167
BsiSI	CCGG	5 cut(s)	249, 583, 1375, 1963, 2245
BslFI	GGGAC	4 cut(s)	3, 230, 877, 1804
BslI	CCNNNNNNNGG	7 cut(s)	26, 392, 924, 1153, 1198, 1603, 1811
BsmAI	GTCTC	3 cut(s)	2150, 2170, 2231
BsmBI	CGTCTC	1 cut(s)	2231
BsmFI	GGGAC	4 cut(s)	3, 230, 877, 1804
BsmI	GAATGC	1 cut(s)	974
BsnI	GGCC	7 cut(s)	207, 891, 1246, 1304, 1860, 2360, 2408
Bso31I	GGTCTC	1 cut(s)	2150
BsoBI	CYCGRG	2 cut(s)	239, 2167
Bsp1286I	GDGCHC	3 cut(s)	1804, 2489, 2508
Bsp1407I	TGTACA	1 cut(s)	850
Bsp143I	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
Bsp1720I	GCTNAGC	2 cut(s)	1203, 1494
Bsp19I	CCATGG	1 cut(s)	54
BspACI	CCGC	6 cut(s)	42, 73, 110, 1082, 1173, 2107
BspANI	GGCC	7 cut(s)	207, 891, 1246, 1304, 1860, 2360, 2408
BspCNI	CTCAG	8 cut(s)	1060, 1195, 1486, 1569, 2113, 2159, 2218, 2311
BspFNI	CGCG	2 cut(s)	73, 213
BspLI	GGNNCC	4 cut(s)	19, 246, 609, 1955
BspMAI	CTGCAG	1 cut(s)	2480
BspMI	ACCTGC	1 cut(s)	2515
BspOI	GCTAGC	1 cut(s)	1211
BspPI	GGATC	4 cut(s)	442, 1092, 1767, 2294
BspT107I	GGYRCC	1 cut(s)	607
BspTI	CTTAAG	2 cut(s)	458, 1721
BspTNI	GGTCTC	1 cut(s)	2150
BsrBI	CCGCTC	1 cut(s)	2107
BsrDI	GCAATG	6 cut(s)	423, 750, 1080, 1257, 1299, 1488
BsrFI	RCCGGY	1 cut(s)	2244
BsrGI	TGTACA	1 cut(s)	850
BsrI	ACTGG	6 cut(s)	51, 208, 885, 1925, 2118, 2285
BssAI	RCCGGY	1 cut(s)	2244
BssECI	CCNNGG	6 cut(s)	54, 102, 247, 316, 919, 1597
BssMI	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
BssNI	GRCGYC	2 cut(s)	50, 228
BssT1I	CCWWGG	3 cut(s)	54, 316, 919
Bst2UI	CCWGG	4 cut(s)	1598, 1806, 2008, 2410
Bst4CI	ACNGT	2 cut(s)	142, 2308
Bst6I	CTCTTC	2 cut(s)	159, 2223
BstACI	GRCGYC	2 cut(s)	50, 228
BstAFI	CTTAAG	2 cut(s)	458, 1721
BstAPI	GCANNNNNTGC	3 cut(s)	1079, 1280, 2521
BstAUI	TGTACA	1 cut(s)	850
BstBAI	YACGTR	1 cut(s)	2090
BstC8I	GCNNGC	5 cut(s)	699, 1209, 1718, 2246, 2266
BstDSI	CCRYGG	1 cut(s)	54
BstEII	GGTNACC	1 cut(s)	823
BstF5I	GGATG	6 cut(s)	112, 163, 409, 2029, 2134, 2538
BstFNI	CGCG	2 cut(s)	73, 213
BstH2I	RGCGCY	3 cut(s)	193, 239, 255
BstHHI	GCGC	4 cut(s)	192, 238, 254, 1058
BstKTI	GATC	9 cut(s)	36, 450, 531, 632, 1087, 1109, 1762, 1899, 2302
BstMAI	GTCTC	3 cut(s)	2150, 2170, 2231
BstMBI	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
BstNI	CCWGG	4 cut(s)	1598, 1806, 2008, 2410
BstNSI	RCATGY	1 cut(s)	542
BstPAI	GACNNNNGTC	2 cut(s)	49, 2162
BstPI	GGTNACC	1 cut(s)	823
BstSCI	CCNGG	7 cut(s)	247, 581, 1373, 1596, 1804, 2006, 2408
BstSFI	CTRYAG	3 cut(s)	885, 1932, 2476
BstSLI	GKGCMC	2 cut(s)	2489, 2508
BstSNI	TACGTA	1 cut(s)	2090
BstUI	CGCG	2 cut(s)	73, 213
BstV1I	GCAGC	3 cut(s)	199, 1283, 2260
BstV2I	GAAGAC	1 cut(s)	545
BstX2I	RGATCY	4 cut(s)	629, 1084, 1759, 2299
BstYI	RGATCY	4 cut(s)	629, 1084, 1759, 2299
BsuI	GTATCC	1 cut(s)	106
BsuRI	GGCC	7 cut(s)	207, 891, 1246, 1304, 1860, 2360, 2408
BtgI	CCRYGG	1 cut(s)	54
BtgZI	GCGATG	2 cut(s)	143, 2136
BtsCI	GGATG	6 cut(s)	112, 163, 409, 2029, 2134, 2538
BtsI	GCAGTG	2 cut(s)	967, 2487
BtsIMutI	CAGTG	8 cut(s)	201, 370, 662, 853, 967, 2111, 2292, 2487
BveI	ACCTGC	1 cut(s)	2515
Cac8I	GCNNGC	5 cut(s)	699, 1209, 1718, 2246, 2266
CaiI	CAGNNNCTG	2 cut(s)	1067, 1931
CfoI	GCGC	4 cut(s)	192, 238, 254, 1058
Cfr10I	RCCGGY	1 cut(s)	2244
Cfr13I	GGNCC	6 cut(s)	17, 206, 244, 427, 890, 2406
CseI	GACGC	2 cut(s)	39, 202
Csp6I	GTAC	5 cut(s)	507, 705, 851, 1103, 2230
CviAII	CATG	6 cut(s)	55, 370, 539, 1314, 1384, 2421
CviQI	GTAC	5 cut(s)	507, 705, 851, 1103, 2230
DpnI	GATC	9 cut(s)	35, 449, 530, 631, 1086, 1108, 1761, 1898, 2301
DpnII	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
DraI	TTTAAA	1 cut(s)	636
DraIII	CACNNNGTG	1 cut(s)	2210
DrdI	GACNNNNNNGTC	1 cut(s)	962
DseDI	GACNNNNNNGTC	1 cut(s)	962
EaeI	YGGCCR	2 cut(s)	1302, 1858
Eam1104I	CTCTTC	2 cut(s)	159, 2223
EarI	CTCTTC	2 cut(s)	159, 2223
EciI	GGCGGA	1 cut(s)	57
Ecl136II	GAGCTC	1 cut(s)	1802
Eco105I	TACGTA	1 cut(s)	2090
Eco130I	CCWWGG	3 cut(s)	54, 316, 919
Eco24I	GRGCYC	1 cut(s)	1804
Eco31I	GGTCTC	1 cut(s)	2150
Eco32I	GATATC	1 cut(s)	588
Eco47I	GGWCC	3 cut(s)	17, 244, 427
Eco47III	AGCGCT	2 cut(s)	191, 237
Eco53kI	GAGCTC	1 cut(s)	1802
Eco57I	CTGAAG	1 cut(s)	580
Eco88I	CYCGRG	2 cut(s)	239, 2167
Eco91I	GGTNACC	1 cut(s)	823
EcoICRI	GAGCTC	1 cut(s)	1802
EcoO109I	RGGNCCY	3 cut(s)	17, 244, 890
EcoO65I	GGTNACC	1 cut(s)	823
EcoRII	CCWGG	4 cut(s)	1596, 1804, 2006, 2408
EcoRV	GATATC	1 cut(s)	588
EcoT14I	CCWWGG	3 cut(s)	54, 316, 919
EcoT22I	ATGCAT	3 cut(s)	135, 1319, 1483
EcoT38I	GRGCYC	1 cut(s)	1804
ErhI	CCWWGG	3 cut(s)	54, 316, 919
Esp3I	CGTCTC	1 cut(s)	2231
FaeI	CATG	6 cut(s)	58, 373, 542, 1317, 1387, 2424
FaqI	GGGAC	4 cut(s)	3, 230, 877, 1804
FatI	CATG	6 cut(s)	54, 369, 538, 1313, 1383, 2420
FauNDI	CATATG	2 cut(s)	905, 1483
FbaI	TGATCA	1 cut(s)	1896
FblI	GTMKAC	1 cut(s)	535
Fnu4HI	GCNGC	4 cut(s)	188, 1173, 1272, 2249
FokI	GGATG	6 cut(s)	119, 170, 416, 2036, 2141, 2545
FriOI	GRGCYC	1 cut(s)	1804
Fsp4HI	GCNGC	4 cut(s)	188, 1173, 1272, 2249
FspBI	CTAG	4 cut(s)	1208, 1284, 1436, 1499
FspI	TGCGCA	1 cut(s)	1057
GlaI	GCGC	4 cut(s)	191, 237, 253, 1057
GluI	GCNGC	4 cut(s)	188, 1173, 1272, 2249
GsuI	CTGGAG	2 cut(s)	1788, 2135
HaeII	RGCGCY	3 cut(s)	193, 239, 255
HaeIII	GGCC	7 cut(s)	207, 891, 1246, 1304, 1860, 2360, 2408
HapII	CCGG	5 cut(s)	249, 583, 1375, 1963, 2245
HgaI	GACGC	2 cut(s)	39, 202
HhaI	GCGC	4 cut(s)	192, 238, 254, 1058
Hin1I	GRCGYC	2 cut(s)	50, 228
Hin1II	CATG	6 cut(s)	58, 373, 542, 1317, 1387, 2424
Hin6I	GCGC	4 cut(s)	190, 236, 252, 1056
HinP1I	GCGC	4 cut(s)	190, 236, 252, 1056
HincII	GTYRAC	2 cut(s)	536, 1611
HindII	GTYRAC	2 cut(s)	536, 1611
HindIII	AAGCTT	2 cut(s)	677, 1718
HpaII	CCGG	5 cut(s)	249, 583, 1375, 1963, 2245
HphI	GGTGA	8 cut(s)	344, 509, 727, 823, 1846, 2033, 2147, 2290
Hpy166II	GTNNAC	5 cut(s)	536, 851, 988, 1611, 2200
Hpy8I	GTNNAC	5 cut(s)	536, 851, 988, 1611, 2200
Hpy99I	CGWCG	2 cut(s)	218, 233
HpyAV	CCTTC	4 cut(s)	8, 282, 494, 1831
HpyCH4III	ACNGT	2 cut(s)	142, 2308
HpyCH4IV	ACGT	4 cut(s)	228, 359, 437, 2089
HpySE526I	ACGT	4 cut(s)	228, 359, 437, 2089
Hsp92I	GRCGYC	2 cut(s)	50, 228
Hsp92II	CATG	6 cut(s)	58, 373, 542, 1317, 1387, 2424
HspAI	GCGC	4 cut(s)	190, 236, 252, 1056
KroI	GCCGGC	1 cut(s)	2244
KroNI	GCCGGC	1 cut(s)	2246
Ksp22I	TGATCA	1 cut(s)	1896
Kzo9I	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
LmnI	GCTCC	3 cut(s)	413, 1807, 2104
Lsp1109I	GCAGC	3 cut(s)	199, 1283, 2260
LweI	GCATC	7 cut(s)	97, 115, 120, 142, 568, 1755, 2524
MaeI	CTAG	4 cut(s)	1208, 1284, 1436, 1499
MaeII	ACGT	4 cut(s)	228, 359, 437, 2089
MaeIII	GTNAC	5 cut(s)	733, 823, 1528, 2109, 2390
MalI	GATC	9 cut(s)	35, 449, 530, 631, 1086, 1108, 1761, 1898, 2301
MbiI	CCGCTC	1 cut(s)	2107
MboI	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
MfeI	CAATTG	1 cut(s)	792
MflI	RGATCY	4 cut(s)	629, 1084, 1759, 2299
MhlI	GDGCHC	3 cut(s)	1804, 2489, 2508
MlsI	TGGCCA	1 cut(s)	1860
MluNI	TGGCCA	1 cut(s)	1860
MlyI	GAGTC	6 cut(s)	53, 77, 92, 661, 2138, 2309
MmeI	TCCRAC	6 cut(s)	319, 760, 856, 1584, 1634, 2327
Mox20I	TGGCCA	1 cut(s)	1860
Mph1103I	ATGCAT	3 cut(s)	135, 1319, 1483
MroNI	GCCGGC	1 cut(s)	2244
MscI	TGGCCA	1 cut(s)	1860
MslI	CAYNNNNRTG	1 cut(s)	1679
Msp20I	TGGCCA	1 cut(s)	1860
MspA1I	CMGCKG	1 cut(s)	761
MspCI	CTTAAG	2 cut(s)	458, 1721
MspI	CCGG	5 cut(s)	249, 583, 1375, 1963, 2245
MspR9I	CCNGG	7 cut(s)	249, 583, 1375, 1598, 1806, 2008, 2410
MunI	CAATTG	1 cut(s)	792
Mva1269I	GAATGC	1 cut(s)	974
MvaI	CCWGG	4 cut(s)	1598, 1806, 2008, 2410
MvnI	CGCG	2 cut(s)	73, 213
NaeI	GCCGGC	1 cut(s)	2246
NciI	CCSGG	3 cut(s)	249, 583, 1375
NcoI	CCATGG	1 cut(s)	54
NdeI	CATATG	2 cut(s)	905, 1483
NdeII	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
NgoMIV	GCCGGC	1 cut(s)	2244
NheI	GCTAGC	1 cut(s)	1207
NlaIII	CATG	6 cut(s)	58, 373, 542, 1317, 1387, 2424
NlaIV	GGNNCC	4 cut(s)	19, 246, 609, 1955
NmeAIII	GCCGAG	1 cut(s)	208
NmuCI	GTSAC	2 cut(s)	2109, 2390
NsbI	TGCGCA	1 cut(s)	1057
NsiI	ATGCAT	3 cut(s)	135, 1319, 1483
NspI	RCATGY	1 cut(s)	542
PaeR7I	CTCGAG	2 cut(s)	239, 2167
PcsI	WCGNNNNNNNCGW	2 cut(s)	180, 237
PctI	GAATGC	1 cut(s)	974
PdiI	GCCGGC	1 cut(s)	2246
PfeI	GAWTC	5 cut(s)	149, 196, 218, 2366, 2417
PflMI	CCANNNNNTGG	1 cut(s)	1811
PfoI	TCCNGGA	1 cut(s)	1373
PkrI	GCNGC	4 cut(s)	189, 1174, 1273, 2250
PleI	GAGTC	6 cut(s)	53, 76, 92, 661, 2138, 2309
PpsI	GAGTC	6 cut(s)	53, 76, 92, 661, 2138, 2309
Ppu21I	YACGTR	1 cut(s)	2090
PpuMI	RGGWCCY	2 cut(s)	17, 244
PshAI	GACNNNNGTC	2 cut(s)	49, 2162
PshBI	ATTAAT	1 cut(s)	390
PsiI	TTATAA	1 cut(s)	1691
Psp124BI	GAGCTC	1 cut(s)	1804
Psp5II	RGGWCCY	2 cut(s)	17, 244
Psp6I	CCWGG	4 cut(s)	1596, 1804, 2006, 2408
PspEI	GGTNACC	1 cut(s)	823
PspGI	CCWGG	4 cut(s)	1596, 1804, 2006, 2408
PspN4I	GGNNCC	4 cut(s)	19, 246, 609, 1955
PspPI	GGNCC	6 cut(s)	17, 206, 244, 427, 890, 2406
PspPPI	RGGWCCY	2 cut(s)	17, 244
PspXI	VCTCGAGB	1 cut(s)	239
PstI	CTGCAG	1 cut(s)	2480
PstNI	CAGNNNCTG	2 cut(s)	1067, 1931
PsuI	RGATCY	4 cut(s)	629, 1084, 1759, 2299
PvuII	CAGCTG	1 cut(s)	761
RsaI	GTAC	5 cut(s)	508, 706, 852, 1104, 2231
RsaNI	GTAC	5 cut(s)	507, 705, 851, 1103, 2230
RseI	CAYNNNNRTG	1 cut(s)	1679
SacI	GAGCTC	1 cut(s)	1804
SalI	GTCGAC	1 cut(s)	534
SatI	GCNGC	4 cut(s)	188, 1173, 1272, 2249
Sau3AI	GATC	9 cut(s)	33, 447, 528, 629, 1084, 1106, 1759, 1896, 2299
Sau96I	GGNCC	6 cut(s)	17, 206, 244, 427, 890, 2406
SchI	GAGTC	6 cut(s)	53, 77, 92, 661, 2138, 2309
ScrFI	CCNGG	7 cut(s)	249, 583, 1375, 1598, 1806, 2008, 2410
SduI	GDGCHC	3 cut(s)	1804, 2489, 2508
SfaNI	GCATC	7 cut(s)	97, 115, 120, 142, 568, 1755, 2524
SfcI	CTRYAG	3 cut(s)	885, 1932, 2476
Sfr274I	CTCGAG	2 cut(s)	239, 2167
SinI	GGWCC	3 cut(s)	17, 244, 427
SlaI	CTCGAG	2 cut(s)	239, 2167
SmiMI	CAYNNNNRTG	1 cut(s)	1679
SmlI	CTYRAG	7 cut(s)	239, 458, 653, 1721, 2075, 2167, 2464
SmoI	CTYRAG	7 cut(s)	239, 458, 653, 1721, 2075, 2167, 2464
SnaBI	TACGTA	1 cut(s)	2090
SsiI	CCGC	6 cut(s)	42, 73, 110, 1082, 1173, 2107
SspMI	CTAG	4 cut(s)	1208, 1284, 1436, 1499
SstI	GAGCTC	1 cut(s)	1804
StyD4I	CCNGG	7 cut(s)	247, 581, 1373, 1596, 1804, 2006, 2408
StyI	CCWWGG	3 cut(s)	54, 316, 919
TaaI	ACNGT	2 cut(s)	142, 2308
TaiI	ACGT	4 cut(s)	231, 362, 440, 2092
TatI	WGTACW	2 cut(s)	850, 2229
TauI	GCSGC	1 cut(s)	1175
TfiI	GAWTC	5 cut(s)	149, 196, 218, 2366, 2417
TscAI	CASTG	8 cut(s)	208, 370, 669, 853, 967, 2118, 2292, 2494
TseFI	GTSAC	2 cut(s)	2109, 2390
TseI	GCWGC	3 cut(s)	187, 1271, 2248
Tsp45I	GTSAC	2 cut(s)	2109, 2390
TspDTI	ATGAA	9 cut(s)	177, 513, 819, 892, 1302, 1372, 1743, 2074, 2409
TspGWI	ACGGA	3 cut(s)	131, 561, 2205
TspRI	CASTG	8 cut(s)	208, 370, 669, 853, 967, 2118, 2292, 2494
Van91I	CCANNNNNTGG	1 cut(s)	1811
Vha464I	CTTAAG	2 cut(s)	458, 1721
VpaK11BI	GGWCC	3 cut(s)	17, 244, 427
VspI	ATTAAT	1 cut(s)	390
XapI	RAATTY	4 cut(s)	857, 1392, 2081, 2494
XceI	RCATGY	1 cut(s)	542
XhoI	CTCGAG	2 cut(s)	239, 2167
XmiI	GTMKAC	1 cut(s)	535
XspI	CTAG	4 cut(s)	1208, 1284, 1436, 1499
ZraI	GACGTC	1 cut(s)	229
Zsp2I	ATGCAT	3 cut(s)	135, 1319, 1483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.