AT4G35370

Contains the following InterPro domains WD40 repeat 2 (InterPro IPR019782), WD40 repeat, conserved site (InterPro IPR019775), WD40 repeat (InterPro IPR001680), G-protein beta WD-40 repeat, region (InterPro IPR020472), WD40 repeat-like-containing domain (InterPro IPR011046), WD40-repeat-containing domain (InterPro IPR017986), WD40 YVTN repeat-like-containing domain (InterPro IPR015943), WD40 repeat, subgroup (InterPro IPR019781)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007129
Physical Location & Seq
Forward (+)
16815138 .. 16817544
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35370.1

Sequence Viewer

Length: 1302 bp
ATGACGATAGTTGCACTTTCATGGATCCCAAAAGAGGCATCAAAGGCTATGCCTGTTGCTGCTGAATCTCCTTGTATGGAAGAAAAGATGGACGATGAAATTCAGAATGCTCAAGTTACTCATGCCAAGTCTGTAGCAAAATCTTTTGGGAAAAGCAAGGTTGCTTCTTCTTCTTCAACAGATGCTGATGAAGTTGTCAAGTTCTTGAAAGAACTCGACATGGATAACTATGATGAAGAGGATGATGAGATTGAACTGTTTAGCTCTGGGCAGGGACACCTTTATTATCCAAGCAATGACATGGATCCATACCTAAAGGATACTGACGGTGACTATGACTCAGAGGATCACGATGACTTGACAATTAGGCCAACCGATTCACTGATCATCTGTGCTGCTATTAAACATGAAGTCAACTATCTCGAGGTTTATGTATATGAGGAATCCGAAAACATTTATCTTCGCAATGACATGATTATATCCGAGCTTCCATTGTGCACAGCATGGCTTGATTGTCCTCTCAAAGGAGGGGGAAAAGGGAACTTTGTAGCTATCGGGACAATGGAGTCCTCTATAGAGATATGGGATCTTGACCTTGTATGTACTTGTGCTACCTTGTGTACAACTGGGACGGATAATTCCCACACTGGTCCAGTAATTGATCTTGCTTGGAACAAGGAGTTTCGGAATATAGTTGCTAGTGGTAGTGAAGACAAAAAAGTGAAGGTTTGGGATGTGGCTACGGGGAAATGCAAGGTTACTATGGAGCATCACGAAAAGAAGGTTCACGCGGTTGCATGGAACAATTATACTCCAGAAGTACTCCTCAGTGGGTCTCGTGATCGTACTGTAGTATTGAAAGATGGAAGGGACCCTTCAAACTCGGGTTTGAAATGGTCTACCGAGGCCAAAGTCGAAAAATTGGCTTGGGATCCTCACAGCGAACACTCCTTTGTGGTGAGTCTCAAAGATGGAACTGTGAAGGGCTTTGATACACGTGCTTCTGATTTATCACCAAGTTTCATTATCCATGCTCATGATAGTGAAGTCTCCTCCATATCATACAACATACATGCCCCCAATCTTTTGGCTACGGGATCTGCGGATGAAAGTGTGAAACTTTGGGATCTTTCAAACAACCAACCATCATGGATTGCTACAAACAAGCCAAATGCTGGAGAAGTTTTCTCGGTCTCATTCTCAGCGGACTGCCCCTTCTTACTCGCTGTTGGTGGCTCAGAGGGGCTAAATGTAAGTGTGGGATACCTTATCAGACACTGCTGTGTCCCGAAGATATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001933 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005794 GO:0006325 GO:0006355 GO:0006356 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010563 GO:0010604 GO:0010605 GO:0010628 GO:0010646 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0033135 GO:0033137 GO:0033139 GO:0033140 GO:0034770 GO:0034773 GO:0034968 GO:0035064 GO:0036211 GO:0042325 GO:0042326 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045893 GO:0045935 GO:0045936 GO:0045943 GO:0046425 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0140030 GO:0140034 GO:1901564 GO:1901836 GO:1901838 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1904892 GO:1990889 GO:2000112 GO:2000736 GO:2000738 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

433

Amino Acids

47.73

Weight (kDa)

4.69

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 177 - 289 9.4e-08 WDR3 second beta-propeller domain
WDR55 PF24796 178 - 284 9.8e-06 WDR55
Beta-prop_THOC3 PF25174 179 - 410 2.8e-31 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 179 - 289 3.1e-17 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 180 - 412 9.2e-08 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 181 - 315 2.5e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 181 - 284 1.5e-14 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 187 - 291 3.9e-08 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 188 - 381 1.1e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 209 - 284 2.5e-06 WDHD1 first WD40 domain
EIF3I PF24805 210 - 416 1.2e-09 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 214 - 290 9.5e-07 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 216 - 414 6.9e-23 Prp19 WD40 domain
Beta-prop_EIPR1 PF23609 248 - 375 2.8e-10 EIPR1 beta-propeller
Beta-prop_Aladin PF25460 256 - 409 2.4e-07 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 264 - 407 2.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 301 - 408 3.3e-09 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 302 - 413 9.4e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 303 - 407 4.8e-09 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 565
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccI GTMKAC 1 cut(s) 899
AccII CGCG 1 cut(s) 791
AciI CCGC 3 cut(s) 791, 1103, 1205
AcsI RAATTY 1 cut(s) 99
AcvI CACGTG 1 cut(s) 998
AfaI GTAC 4 cut(s) 604, 622, 822, 847
AfiI CCNNNNNNNGG 3 cut(s) 34, 524, 1175
AflIII ACRYGT 1 cut(s) 995
AgsI TTSAA 7 cut(s) 177, 208, 254, 859, 879, 892, 1134
AleI CACNNNNGTG 1 cut(s) 1281
AluBI AGCT 3 cut(s) 264, 487, 551
AluI AGCT 3 cut(s) 264, 487, 551
Alw21I GWGCWC 1 cut(s) 500
Alw26I GTCTC 4 cut(s) 840, 968, 1054, 1198
Alw44I GTGCAC 1 cut(s) 496
AlwNI CAGNNNCTG 2 cut(s) 185, 1278
Ama87I CYCGRG 2 cut(s) 422, 883
AoxI GGCC 2 cut(s) 368, 906
ApaLI GTGCAC 1 cut(s) 496
ApeKI GCWGC 2 cut(s) 59, 395
ApoI RAATTY 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 650, 871
AsuHPI GGTGA 3 cut(s) 341, 970, 1005
AvaI CYCGRG 2 cut(s) 422, 883
AvaII GGWCC 2 cut(s) 650, 871
BaeGI GKGCMC 1 cut(s) 500
BamHI GGATCC 3 cut(s) 24, 304, 931
BauI CACGAG 1 cut(s) 837
BbrPI CACGTG 1 cut(s) 998
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 46, 382
BccI CCATC 4 cut(s) 82, 857, 965, 1153
BciVI GTATCC 2 cut(s) 313, 1256
BclI TGATCA 1 cut(s) 384
BcoDI GTCTC 4 cut(s) 840, 968, 1054, 1198
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 3 cut(s) 132, 573, 849
BfuI GTATCC 2 cut(s) 313, 1256
BisI GCNGC 2 cut(s) 60, 396
BlsI GCNGC 2 cut(s) 61, 397
BmcAI AGTACT 1 cut(s) 822
Bme18I GGWCC 2 cut(s) 650, 871
BmeT110I CYCGRG 2 cut(s) 422, 883
BmgT120I GGNCC 2 cut(s) 650, 871
BmiI GGNNCC 5 cut(s) 26, 306, 872, 873, 933
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 3 cut(s) 47, 172, 778
BmuI ACTGGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 717
BpmI CTGGAG 2 cut(s) 798, 1197
BpuEI CTTGAG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 998
BsaI GGTCTC 2 cut(s) 840, 1198
BsaJI CCNNGG 1 cut(s) 903
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 524, 1175
Bse1I ACTGG 3 cut(s) 631, 652, 653
Bse3DI GCAATG 2 cut(s) 301, 472
BseDI CCNNGG 1 cut(s) 903
BseGI GGATG 3 cut(s) 247, 739, 1111
BseLI CCNNNNNNNGG 3 cut(s) 34, 524, 1175
BseMI GCAATG 2 cut(s) 301, 472
BseMII CTCAG 4 cut(s) 354, 841, 1215, 1251
BseNI ACTGG 3 cut(s) 631, 652, 653
BseRI GAGGAG 2 cut(s) 815, 1042
BseSI GKGCMC 1 cut(s) 500
BseXI GCAGC 2 cut(s) 46, 382
Bsh1236I CGCG 1 cut(s) 791
BshFI GGCC 2 cut(s) 370, 908
BsiHKAI GWGCWC 1 cut(s) 500
BsiHKCI CYCGRG 2 cut(s) 422, 883
BslFI GGGAC 5 cut(s) 288, 571, 643, 884, 1271
BslI CCNNNNNNNGG 3 cut(s) 34, 524, 1175
BsmAI GTCTC 4 cut(s) 840, 968, 1054, 1198
BsmFI GGGAC 5 cut(s) 288, 571, 643, 884, 1271
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 2 cut(s) 370, 908
Bso31I GGTCTC 2 cut(s) 840, 1198
BsoBI CYCGRG 2 cut(s) 422, 883
Bsp1286I GDGCHC 1 cut(s) 500
Bsp1407I TGTACA 1 cut(s) 620
BspACI CCGC 3 cut(s) 791, 1103, 1205
BspANI GGCC 2 cut(s) 370, 908
BspCNI CTCAG 4 cut(s) 353, 840, 1214, 1250
BspFNI CGCG 1 cut(s) 791
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 5 cut(s) 26, 306, 872, 873, 933
BspTNI GGTCTC 2 cut(s) 840, 1198
BsrDI GCAATG 2 cut(s) 301, 472
BsrGI TGTACA 1 cut(s) 620
BsrI ACTGG 3 cut(s) 631, 652, 653
BssECI CCNNGG 1 cut(s) 903
BssSI CACGAG 1 cut(s) 837
Bst2BI CACGAG 1 cut(s) 837
Bst4CI ACNGT 4 cut(s) 258, 329, 850, 979
Bst6I CTCTTC 1 cut(s) 231
BstAUI TGTACA 1 cut(s) 620
BstBAI YACGTR 1 cut(s) 998
BstDEI CTNAG 4 cut(s) 340, 827, 1201, 1237
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 3 cut(s) 247, 739, 1111
BstFNI CGCG 1 cut(s) 791
BstMAI GTCTC 4 cut(s) 840, 968, 1054, 1198
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 1076
BstSFI CTRYAG 3 cut(s) 132, 573, 849
BstSLI GKGCMC 1 cut(s) 500
BstUI CGCG 1 cut(s) 791
BstV1I GCAGC 2 cut(s) 46, 382
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 6 cut(s) 24, 304, 586, 931, 1097, 1126
BstXI CCANNNNNNTGG 1 cut(s) 1087
BstYI RGATCY 6 cut(s) 24, 304, 586, 931, 1097, 1126
BsuI GTATCC 2 cut(s) 313, 1256
BsuRI GGCC 2 cut(s) 370, 908
BtsCI GGATG 3 cut(s) 247, 739, 1111
BtsI GCAGTG 1 cut(s) 1276
BtsIMutI CAGTG 4 cut(s) 380, 645, 835, 1276
CaiI CAGNNNCTG 2 cut(s) 185, 1278
CciI TCATGA 1 cut(s) 1036
Cfr13I GGNCC 2 cut(s) 650, 871
Csp6I GTAC 4 cut(s) 603, 621, 821, 846
CviQI GTAC 4 cut(s) 603, 621, 821, 846
DdeI CTNAG 4 cut(s) 340, 827, 1201, 1237
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco31I GGTCTC 2 cut(s) 840, 1198
Eco47I GGWCC 2 cut(s) 650, 871
Eco72I CACGTG 1 cut(s) 998
Eco88I CYCGRG 2 cut(s) 422, 883
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 871
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FaqI GGGAC 5 cut(s) 288, 571, 643, 884, 1271
FbaI TGATCA 1 cut(s) 384
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 2 cut(s) 60, 396
FokI GGATG 3 cut(s) 254, 746, 1118
Fsp4HI GCNGC 2 cut(s) 60, 396
FspBI CTAG 1 cut(s) 699
GluI GCNGC 2 cut(s) 60, 396
GsuI CTGGAG 2 cut(s) 798, 1197
HaeIII GGCC 2 cut(s) 370, 908
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 6 cut(s) 65, 338, 377, 443, 566, 961
HphI GGTGA 3 cut(s) 341, 970, 1005
Hpy166II GTNNAC 5 cut(s) 415, 498, 621, 787, 900
Hpy188I TCNGA 8 cut(s) 105, 343, 448, 484, 687, 1006, 1240, 1274
Hpy8I GTNNAC 5 cut(s) 415, 498, 621, 787, 900
HpyAV CCTTC 6 cut(s) 718, 775, 861, 885, 976, 1225
HpyCH4III ACNGT 4 cut(s) 258, 329, 850, 979
HpyCH4IV ACGT 1 cut(s) 997
HpyCH4V TGCA 4 cut(s) 14, 498, 753, 797
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 4 cut(s) 340, 827, 1201, 1237
HpySE526I ACGT 1 cut(s) 997
KflI GGGWCCC 1 cut(s) 871
Ksp22I TGATCA 1 cut(s) 384
LmnI GCTCC 1 cut(s) 766
LpnPI CCDG 8 cut(s) 66, 252, 257, 612, 633, 666, 828, 1161
Lsp1109I GCAGC 2 cut(s) 46, 382
LweI GCATC 3 cut(s) 47, 172, 778
MaeI CTAG 1 cut(s) 699
MaeII ACGT 1 cut(s) 997
MaeIII GTNAC 3 cut(s) 115, 329, 757
MboII GAAGA 7 cut(s) 92, 159, 162, 165, 248, 452, 722
MflI RGATCY 6 cut(s) 24, 304, 586, 931, 1097, 1126
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 6 cut(s) 99, 363, 637, 657, 805, 920
MlyI GAGTC 3 cut(s) 332, 575, 970
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 4 cut(s) 19, 1035, 1041, 1281
MspA1I CMGCKG 1 cut(s) 1205
Mva1269I GAATGC 1 cut(s) 112
MvnI CGCG 1 cut(s) 791
MwoI GCNNNNNNNGC 1 cut(s) 44
NlaIV GGNNCC 5 cut(s) 26, 306, 872, 873, 933
NmuCI GTSAC 1 cut(s) 329
NspI RCATGY 1 cut(s) 1076
OliI CACNNNNGTG 1 cut(s) 1281
PaeR7I CTCGAG 1 cut(s) 422
PagI TCATGA 1 cut(s) 1036
PctI GAATGC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 65, 377, 443
PflMI CCANNNNNTGG 1 cut(s) 1175
PkrI GCNGC 2 cut(s) 61, 397
PleI GAGTC 3 cut(s) 332, 574, 969
PmaCI CACGTG 1 cut(s) 998
PmlI CACGTG 1 cut(s) 998
PpsI GAGTC 3 cut(s) 332, 574, 969
Ppu21I YACGTR 1 cut(s) 998
PpuMI RGGWCCY 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 871
PspCI CACGTG 1 cut(s) 998
PspN4I GGNNCC 5 cut(s) 26, 306, 872, 873, 933
PspPI GGNCC 2 cut(s) 650, 871
PspPPI RGGWCCY 1 cut(s) 871
PstNI CAGNNNCTG 2 cut(s) 185, 1278
PsuI RGATCY 6 cut(s) 24, 304, 586, 931, 1097, 1126
RsaI GTAC 4 cut(s) 604, 622, 822, 847
RsaNI GTAC 4 cut(s) 603, 621, 821, 846
RseI CAYNNNNRTG 4 cut(s) 19, 1035, 1041, 1281
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 2 cut(s) 60, 396
Sau96I GGNCC 2 cut(s) 650, 871
ScaI AGTACT 1 cut(s) 822
SchI GAGTC 3 cut(s) 332, 575, 970
SduI GDGCHC 1 cut(s) 500
SfaNI GCATC 3 cut(s) 47, 172, 778
SfcI CTRYAG 3 cut(s) 132, 573, 849
Sfr274I CTCGAG 1 cut(s) 422
SinI GGWCC 2 cut(s) 650, 871
SlaI CTCGAG 1 cut(s) 422
SmiMI CAYNNNNRTG 4 cut(s) 19, 1035, 1041, 1281
SmlI CTYRAG 2 cut(s) 111, 422
SmoI CTYRAG 2 cut(s) 111, 422
Sse9I AATT 6 cut(s) 99, 363, 637, 657, 805, 920
SsiI CCGC 3 cut(s) 791, 1103, 1205
SspMI CTAG 1 cut(s) 699
TaaI ACNGT 4 cut(s) 258, 329, 850, 979
TaiI ACGT 1 cut(s) 1000
TaqI TCGA 3 cut(s) 216, 423, 915
TaqII GACCGA 1 cut(s) 1180
TasI AATT 6 cut(s) 99, 363, 637, 657, 805, 920
TatI WGTACW 3 cut(s) 602, 620, 820
TfiI GAWTC 3 cut(s) 65, 377, 443
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 4 cut(s) 387, 652, 835, 1283
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 2 cut(s) 59, 395
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 7 cut(s) 9, 111, 204, 249, 423, 1012, 1122
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 4 cut(s) 387, 652, 835, 1283
Van91I CCANNNNNTGG 1 cut(s) 1175
VneI GTGCAC 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 650, 871
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 1 cut(s) 99
XceI RCATGY 1 cut(s) 1076
XhoI CTCGAG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 899
XspI CTAG 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.