Rh2BG142000

Periodic tryptophan protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007129
Physical Location & Seq
Forward (+)
12182924 .. 12187994
5071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG142000.1

Sequence Viewer

Length: 1260 bp
ATGTTTCCTCTTAAAAATGTCATTAAAAATGTCATTAAGATTTTAAACCTCACAGTGCATCTTTTTTTATATTTGACTACAGGCATTGATCTGTTTAGTGGTGGGTATGGGGACCTTTACTACCCAAGTAATGATATGGATCCATATCTCAAGAAGGACAAGGATGATGACGAGGATTCTGAAGATAGTGAGGACATGACCATCAACCCAAATGATGCAGTCATAGTTTGTGCTCGCAATGAGGATGATATCAGTCAGCTTGAGATTTGGCTGTATACTGAAACAGAAGATGGCGAACCAGATGTTTATGTTCACCATGACATCATCATTTCAGCATTTCCCCTTTGCACAGCATGGCTTGATTGCCCTCTCAAAGGTGGAGACAAAGGGAATTTTATAGCTGTTGGTTTGATGGGTGAACCTACCATTGAGATATGGGATCTTGACATTATTGATGAAGTACAACCATGTGTGGTCCTGGGTGGTATTGCTGAGAAGAAGAAAAAGAAAGGAAAAAAGACCTCAATCAAATACAAAGAAGACAGTCACACAGATTCTGTTCTGGGGCTTGCTTGGAATAAGGAGTACAGAAATATACTGGCCAGTGCAAGTGCTGACAAACAAGTTAAAATTTGGGATGTGGCTACTGGAAAATGTAACATTACTATGGAGCATCATACAGACAAGGTTCAGGCGGTTGCATGGAATCATTTTGCCCCTCAAGTTCTTCTTAGTGGATCTTTTGATCATTCAATTGTCTTGAAAGATGGAAGGGTGCCAACACATTCTGGTTATAAGTGGACAGTGACAGCTGATGTAGAAAGCTTAGCATGGGATCCACACACTGAGCATTCATTTGTGGTGAGTCTTGAAGATGGTACAGTCCAAGGTTTTGATATTCGGGCCGCCACTTCTGCTCCTACTACTGAATCTAAACCCAGTTTTACTCTTCATGCACACGACAAAGCTGTTTGTGGAATATCCTATAATCCTTTGGCACCTAATCTTCTTGCAACTGGATCCACAGATAAAATGGTGAAGCTTTGGGATTTATCAAATAATCAACCTTCATGCATTGCATCCATGAATCCTAAAGCAGGAGCTGTGTTTTCTATTTCTTTCTCACAAGATAACCCCTTTTTGCTGGCCATGGGAGGCTCAAAAGGGAAATTGGAAGTATGGGATACATCGTATGATGCTGCAGTTGCCCAAAGATTTGGGAGTTACATCAAGAAGAGCAGACCCCAGTCTGGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001933 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005794 GO:0006325 GO:0006355 GO:0006356 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010563 GO:0010604 GO:0010605 GO:0010628 GO:0010646 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0033135 GO:0033137 GO:0033139 GO:0033140 GO:0034770 GO:0034773 GO:0034968 GO:0035064 GO:0036211 GO:0042325 GO:0042326 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045893 GO:0045935 GO:0045936 GO:0045943 GO:0046425 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0140030 GO:0140034 GO:1901564 GO:1901836 GO:1901838 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1904892 GO:1990889 GO:2000112 GO:2000736 GO:2000738 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

419

Amino Acids

46.2

Weight (kDa)

5.01

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 140 - 238 1.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 174 - 403 7.1e-34 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 175 - 283 6.9e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 181 - 256 1.5e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 181 - 256 6.3e-11 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 181 - 386 1.6e-11 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 183 - 395 9.7e-23 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 232 - 396 4.4e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 269 - 403 2.4e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 270 - 397 1.2e-10 WDR3 first beta-propeller domain
Beta-prop_RIG_2nd PF23775 270 - 364 2.7e-06 RIG second beta-propeller
WD40_CDC20-Fz PF24807 270 - 401 2e-17 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 271 - 398 2.6e-12 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 273 - 349 4.4e-09 EIPR1 beta-propeller
Beta-prop_TEP1_2nd PF25047 273 - 398 8.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 287 - 396 6.8e-07 WDR36/Utp21 first beta-propeller
WD40 PF00400 312 - 350 4.4e-06 WD domain, G-beta repeat
WDR55 PF24796 317 - 399 8.3e-06 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AccB1I GGYRCC 2 cut(s) 775, 997
AccI GTMKAC 1 cut(s) 275
AciI CCGC 2 cut(s) 695, 906
AclWI GGATC 8 cut(s) 134, 147, 447, 745, 830, 843, 1014, 1027
AcoI YGGCCR 2 cut(s) 600, 1146
AcsI RAATTY 2 cut(s) 391, 630
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 3 cut(s) 462, 587, 880
AfiI CCNNNNNNNGG 5 cut(s) 374, 1097, 1250, 1251, 1252
AgsI TTSAA 3 cut(s) 753, 763, 872
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 7 cut(s) 259, 401, 812, 825, 968, 1042, 1103
AluI AGCT 7 cut(s) 259, 401, 812, 825, 968, 1042, 1103
Alw21I GWGCWC 1 cut(s) 235
Alw26I GTCTC 1 cut(s) 375
AlwI GGATC 8 cut(s) 134, 147, 447, 745, 830, 843, 1014, 1027
AlwNI CAGNNNCTG 2 cut(s) 557, 1103
AoxI GGCC 3 cut(s) 600, 903, 1146
ApeKI GCWGC 1 cut(s) 1199
ApoI RAATTY 2 cut(s) 391, 630
AspS9I GGNCC 3 cut(s) 112, 475, 903
AsuHPI GGTGA 4 cut(s) 305, 428, 874, 1048
AvaII GGWCC 2 cut(s) 112, 475
BalI TGGCCA 2 cut(s) 602, 1148
BamHI GGATCC 3 cut(s) 139, 835, 1019
BanI GGYRCC 2 cut(s) 775, 997
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 235
BbvI GCAGC 1 cut(s) 1186
BccI CCATC 5 cut(s) 209, 284, 406, 761, 869
BciT130I CCWGG 1 cut(s) 479
BciVI GTATCC 1 cut(s) 1177
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 1 cut(s) 375
BfmI CTRYAG 2 cut(s) 78, 1200
BfuI GTATCC 1 cut(s) 1177
BisI GCNGC 2 cut(s) 906, 1200
BlpI GCTNAGC 1 cut(s) 826
BlsI GCNGC 2 cut(s) 907, 1201
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 2 cut(s) 112, 475
BmgT120I GGNCC 3 cut(s) 112, 475, 903
BmiI GGNNCC 6 cut(s) 113, 141, 777, 837, 999, 1021
BmrFI CCNGG 1 cut(s) 479
BmrI ACTGGG 2 cut(s) 933, 1240
BmsI GCATC 5 cut(s) 67, 205, 682, 1088, 1186
BmuI ACTGGG 2 cut(s) 933, 1240
BoxI GACNNNNGTC 1 cut(s) 1246
BpiI GAAGAC 1 cut(s) 546
Bpu1102I GCTNAGC 1 cut(s) 826
BpuEI CTTGAG 3 cut(s) 134, 281, 705
BsaBI GATNNNNATC 2 cut(s) 138, 144
BsaJI CCNNGG 3 cut(s) 478, 886, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bsc4I CCNNNNNNNGG 5 cut(s) 374, 1097, 1250, 1251, 1252
Bse1I ACTGG 6 cut(s) 603, 603, 652, 939, 1021, 1246
Bse3DI GCAATG 2 cut(s) 244, 1074
Bse8I GATNNNNATC 2 cut(s) 138, 144
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 3 cut(s) 478, 886, 1149
BseGI GGATG 4 cut(s) 169, 250, 643, 1079
BseJI GATNNNNATC 2 cut(s) 138, 144
BseLI CCNNNNNNNGG 5 cut(s) 374, 1097, 1250, 1251, 1252
BseMI GCAATG 2 cut(s) 244, 1074
BseMII CTCAG 2 cut(s) 483, 837
BseNI ACTGG 6 cut(s) 603, 603, 652, 939, 1021, 1246
BseXI GCAGC 1 cut(s) 1186
BshFI GGCC 3 cut(s) 602, 905, 1148
BshNI GGYRCC 2 cut(s) 775, 997
BsiHKAI GWGCWC 1 cut(s) 235
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 5 cut(s) 374, 1097, 1250, 1251, 1252
BsmAI GTCTC 1 cut(s) 375
BsmFI GGGAC 1 cut(s) 125
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 3 cut(s) 602, 905, 1148
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
Bsp1720I GCTNAGC 1 cut(s) 826
Bsp19I CCATGG 1 cut(s) 1149
BspACI CCGC 2 cut(s) 695, 906
BspANI GGCC 3 cut(s) 602, 905, 1148
BspCNI CTCAG 2 cut(s) 484, 838
BspLI GGNNCC 6 cut(s) 113, 141, 777, 837, 999, 1021
BspMAI CTGCAG 1 cut(s) 1204
BspPI GGATC 8 cut(s) 134, 147, 447, 745, 830, 843, 1014, 1027
BspQI GCTCTTC 1 cut(s) 1229
BspT107I GGYRCC 2 cut(s) 775, 997
BsrDI GCAATG 2 cut(s) 244, 1074
BsrI ACTGG 6 cut(s) 603, 603, 652, 939, 1021, 1246
BssECI CCNNGG 3 cut(s) 478, 886, 1149
BssMI GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
BssNAI GTATAC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 886, 1149
Bst1107I GTATAC 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 4 cut(s) 55, 545, 805, 883
Bst6I CTCTTC 2 cut(s) 954, 1229
BstC8I GCNNGC 3 cut(s) 235, 570, 1146
BstDEI CTNAG 4 cut(s) 492, 731, 826, 846
BstDSI CCRYGG 1 cut(s) 1149
BstENI CCTNNNNNAGG 2 cut(s) 372, 1095
BstF5I GGATG 4 cut(s) 169, 250, 643, 1079
BstKTI GATC 7 cut(s) 91, 142, 442, 740, 748, 838, 1022
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
BstMWI GCNNNNNNNGC 2 cut(s) 914, 1205
BstNI CCWGG 1 cut(s) 479
BstPAI GACNNNNGTC 1 cut(s) 1246
BstSCI CCNGG 1 cut(s) 477
BstSFI CTRYAG 2 cut(s) 78, 1200
BstV1I GCAGC 1 cut(s) 1186
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 5 cut(s) 139, 439, 737, 835, 1019
BstXI CCANNNNNNTGG 1 cut(s) 1217
BstYI RGATCY 5 cut(s) 139, 439, 737, 835, 1019
BstZ17I GTATAC 1 cut(s) 276
BsuI GTATCC 1 cut(s) 1177
BsuRI GGCC 3 cut(s) 602, 905, 1148
BtgI CCRYGG 1 cut(s) 1149
BtsCI GGATG 4 cut(s) 169, 250, 643, 1079
BtsIMutI CAGTG 4 cut(s) 60, 610, 810, 843
Cac8I GCNNGC 3 cut(s) 235, 570, 1146
CaiI CAGNNNCTG 2 cut(s) 557, 1103
Cfr13I GGNCC 3 cut(s) 112, 475, 903
Csp6I GTAC 3 cut(s) 461, 586, 879
CviQI GTAC 3 cut(s) 461, 586, 879
DdeI CTNAG 4 cut(s) 492, 731, 826, 846
DpnI GATC 7 cut(s) 90, 141, 441, 739, 747, 837, 1021
DpnII GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
DraI TTTAAA 1 cut(s) 45
EaeI YGGCCR 2 cut(s) 600, 1146
Eam1104I CTCTTC 2 cut(s) 954, 1229
EarI CTCTTC 2 cut(s) 954, 1229
Eco130I CCWWGG 2 cut(s) 886, 1149
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 112, 475
Eco57I CTGAAG 1 cut(s) 201
EcoNI CCTNNNNNAGG 2 cut(s) 372, 1095
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 477
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 886, 1149
EcoT22I ATGCAT 1 cut(s) 1076
ErhI CCWWGG 2 cut(s) 886, 1149
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 125
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 2 cut(s) 906, 1200
FokI GGATG 4 cut(s) 176, 257, 650, 1066
Fsp4HI GCNGC 2 cut(s) 906, 1200
GluI GCNGC 2 cut(s) 906, 1200
HaeIII GGCC 3 cut(s) 602, 905, 1148
HindIII AAGCTT 2 cut(s) 823, 1040
HinfI GANTC 6 cut(s) 176, 554, 706, 865, 929, 1087
HphI GGTGA 4 cut(s) 305, 428, 874, 1048
Hpy166II GTNNAC 4 cut(s) 276, 313, 419, 801
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 5 cut(s) 151, 443, 760, 869, 1231
Hpy8I GTNNAC 4 cut(s) 276, 313, 419, 801
HpyAV CCTTC 3 cut(s) 148, 765, 1077
HpyCH4III ACNGT 4 cut(s) 55, 545, 805, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 914, 1205
HpyF3I CTNAG 4 cut(s) 492, 731, 826, 846
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
LguI GCTCTTC 1 cut(s) 1229
LmnI GCTCC 3 cut(s) 670, 922, 1100
Lsp1109I GCAGC 1 cut(s) 1186
LweI GCATC 5 cut(s) 67, 205, 682, 1088, 1186
MaeIII GTNAC 4 cut(s) 545, 656, 805, 1223
MalI GATC 7 cut(s) 90, 141, 441, 739, 747, 837, 1021
MboI GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 5 cut(s) 139, 439, 737, 835, 1019
MhlI GDGCHC 1 cut(s) 235
MlsI TGGCCA 2 cut(s) 602, 1148
MluCI AATT 4 cut(s) 391, 630, 753, 1169
MluNI TGGCCA 2 cut(s) 602, 1148
MlyI GAGTC 1 cut(s) 874
MnlI CCTC 9 cut(s) 18, 59, 166, 184, 235, 378, 532, 729, 1148
Mox20I TGGCCA 2 cut(s) 602, 1148
Mph1103I ATGCAT 1 cut(s) 1076
MscI TGGCCA 2 cut(s) 602, 1148
MseI TTAA 5 cut(s) 12, 24, 36, 44, 627
MslI CAYNNNNRTG 1 cut(s) 665
Msp20I TGGCCA 2 cut(s) 602, 1148
MspA1I CMGCKG 1 cut(s) 812
MspR9I CCNGG 1 cut(s) 479
MunI CAATTG 1 cut(s) 753
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 2 cut(s) 914, 1205
NcoI CCATGG 1 cut(s) 1149
NdeII GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
NlaIV GGNNCC 6 cut(s) 113, 141, 777, 837, 999, 1021
NmuCI GTSAC 2 cut(s) 545, 805
NsiI ATGCAT 1 cut(s) 1076
PciSI GCTCTTC 1 cut(s) 1229
PctI GAATGC 1 cut(s) 850
PfeI GAWTC 5 cut(s) 176, 554, 706, 929, 1087
PkrI GCNGC 2 cut(s) 907, 1201
PleI GAGTC 1 cut(s) 873
PpsI GAGTC 1 cut(s) 873
PpuMI RGGWCCY 1 cut(s) 112
PshAI GACNNNNGTC 1 cut(s) 1246
PsiI TTATAA 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 6 cut(s) 113, 141, 777, 837, 999, 1021
PspPI GGNCC 3 cut(s) 112, 475, 903
PspPPI RGGWCCY 1 cut(s) 112
PstI CTGCAG 1 cut(s) 1204
PstNI CAGNNNCTG 2 cut(s) 557, 1103
PsuI RGATCY 5 cut(s) 139, 439, 737, 835, 1019
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 3 cut(s) 462, 587, 880
RsaNI GTAC 3 cut(s) 461, 586, 879
RseI CAYNNNNRTG 1 cut(s) 665
SapI GCTCTTC 1 cut(s) 1229
SaqAI TTAA 5 cut(s) 12, 24, 36, 44, 627
SatI GCNGC 2 cut(s) 906, 1200
Sau3AI GATC 7 cut(s) 88, 139, 439, 737, 745, 835, 1019
Sau96I GGNCC 3 cut(s) 112, 475, 903
SchI GAGTC 1 cut(s) 874
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 5 cut(s) 67, 205, 682, 1088, 1186
SfcI CTRYAG 2 cut(s) 78, 1200
SinI GGWCC 2 cut(s) 112, 475
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 3 cut(s) 149, 260, 720
SmoI CTYRAG 3 cut(s) 149, 260, 720
Sse9I AATT 4 cut(s) 391, 630, 753, 1169
SsiI CCGC 2 cut(s) 695, 906
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 2 cut(s) 886, 1149
TaaI ACNGT 4 cut(s) 55, 545, 805, 883
TasI AATT 4 cut(s) 391, 630, 753, 1169
TatI WGTACW 2 cut(s) 460, 585
TauI GCSGC 1 cut(s) 908
TfiI GAWTC 5 cut(s) 176, 554, 706, 929, 1087
Tru1I TTAA 5 cut(s) 12, 24, 36, 44, 627
Tru9I TTAA 5 cut(s) 12, 24, 36, 44, 627
TscAI CASTG 4 cut(s) 60, 610, 810, 850
TseFI GTSAC 2 cut(s) 545, 805
TseI GCWGC 1 cut(s) 1199
Tsp45I GTSAC 2 cut(s) 545, 805
TspDTI ATGAA 5 cut(s) 471, 843, 941, 1059, 1100
TspRI CASTG 4 cut(s) 60, 610, 810, 850
VpaK11BI GGWCC 2 cut(s) 112, 475
XagI CCTNNNNNAGG 2 cut(s) 372, 1095
XapI RAATTY 2 cut(s) 391, 630
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 275
Zsp2I ATGCAT 1 cut(s) 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.