MD14G1085200.v1.1

Periodic tryptophan protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007129
Physical Location & Seq
Forward (+)
10358323 .. 10363284
4962 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1085200.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGATATCAGCAATTTCGTGGGTGCCCAAAGGGGTTTCGAAGCCGGTTCCCTCCGTCGCCGACCCGCCCACCAAAGAGGAAATCGAAGAGCTGATTAAAACCGGCGCCTTGGAGAAAAGTGGAGATGCTGAAAGTGAGGACTATGATGGAGAAATGGATGTTGAAACCCCCAAGCAGGGTGATCAAGTCTCCCAAGCATTAGAGGTGGCAAAAGCACTCGGAAGAGCTCCTAAAGTTACCGAGCCAGGGGCCAAGTTTGATGACATAGCCGATGGATTGAGGGAACTCAACATGGAACATTATGACGACGAGGATGATGGCATTGACGTGTTTAGTAGTGGGGTTGGGGACCTTTACTACCCAAGTAACGATATGGATCCATATCTAAAGAAGGAAAAGGATGATGACGAGGATTCTGAAGACAGTGATGACATGACAATTAATCCAAGTGATGCAGTCATAGTTTGCGCCCGCAATGAGGATGAAGTCAGCCAGCTTGAGATATGGCTGTATGTTGAATCAGAAGATGGTGAAGCAGATGTTTTTGTTCACCATGACGTCATCCTTTCATCATTTCCCCTTTGCACAGCATGGCTTGATTGTCCTCTCAAAGGTGGAGACAAAGGGAACTTCGTTGCTGTTGGTTTAATGGATGAACCTTCCATTGAGATATGGGATCTTGACATTATTGATGAAGTGCAACCATGCGTGATATTGGGTGGTATTGCTGAGAAGAAAAAAAAGAAAGGAAAGAAGGTATCAATCAAATACAAAGAAGACAGTCACAGAGATTCAGTTCTCGGGCTTGCTTGGAATAAGGAGTACAGAAATATACTTGCTAGTGCAAGTGCTGACAAACAAGTTAAGATTTGGGATGTGGCTACCGGAAAATGTAATATTACCATGGAGCACCATACAGACAAGGTTCAGGCAGTTGCATGGAATCATTTTGCCCCGCAAGTTCTTCTCAGTGGTTCTTTTGATCATACAGTAGTCTTGAAAGATGGGAGGGTGCCATCACATTCTGGGTATAAGTGGACAGTCACAGCTGATGTAGAAAGCTTAGCATGGGATCCACACACTGAGCATTCATTTGTGGTGAGCCTAGAAGATGGCACAATCAAAGGGTTTGATATTCGGGCTGCCACATCTGCTCCTACTTCCGAGTCTAAACCGAGTTTTACTCTTCATGCTCATGACAAAGCTGTTTGCTCAATCTCATATAACCCTGCAGCACCTAATCTTCTTGCAACTGGATCCACAGATAAAATGGTAAAGCTTTGGGATTTGTCAAACAACCAACCTTCATGCATTTCATCCAGGAATCCTAAAGCAGGAGCTGTCTTTTCTATTTCGTTTGCGGAAGATAACCCTTTTTTGCTGGCCATAGGAGGCTCAAAAGGGAAACTGGAAGTATGGGATACATCATATGATGCTGCGGTTGCCCAAAGATTTGGGAGTTACTACAAGAAGAGCAGAACCCAAGCTGGACCTTCATCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001933 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005794 GO:0006325 GO:0006355 GO:0006356 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010563 GO:0010604 GO:0010605 GO:0010628 GO:0010646 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018023 GO:0018193 GO:0018205 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0033135 GO:0033137 GO:0033139 GO:0033140 GO:0034770 GO:0034773 GO:0034968 GO:0035064 GO:0036211 GO:0042325 GO:0042326 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045893 GO:0045935 GO:0045936 GO:0045943 GO:0046425 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0140030 GO:0140034 GO:1901564 GO:1901836 GO:1901838 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1904892 GO:1990889 GO:2000112 GO:2000736 GO:2000738 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

502

Amino Acids

54.73

Weight (kDa)

4.62

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 208 - 315 1.6e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 249 - 382 7.9e-12 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 253 - 362 2.6e-14 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 257 - 482 3.1e-32 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 259 - 335 1.6e-14 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 260 - 466 1.2e-09 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 263 - 474 1.5e-21 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 321 - 475 4e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 326 - 475 1.9e-06 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 347 - 482 1.1e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 349 - 476 2.7e-10 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 349 - 480 3.6e-16 CDC20/Fizzy WD40 domain
Beta-prop_RIG_2nd PF23775 350 - 449 4e-07 RIG second beta-propeller
Beta-prop_WDR5 PF25175 350 - 477 8.8e-12 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 352 - 428 2.1e-09 EIPR1 beta-propeller
Beta-prop_TEP1_2nd PF25047 370 - 484 4e-06 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 371 - 475 3.6e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 391 - 429 6e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 561
AccB1I GGYRCC 3 cut(s) 22, 104, 1012
AciI CCGC 5 cut(s) 65, 472, 956, 1361, 1439
AclWI GGATC 7 cut(s) 371, 384, 684, 1067, 1080, 1251, 1264
AcoI YGGCCR 1 cut(s) 1383
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 2 cut(s) 105, 558
AfaI GTAC 1 cut(s) 824
AfiI CCNNNNNNNGG 7 cut(s) 175, 176, 246, 478, 611, 1334, 1499
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 3 cut(s) 164, 518, 1000
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 2 cut(s) 244, 1319
AluBI AGCT 9 cut(s) 91, 227, 496, 1049, 1062, 1205, 1279, 1340, 1487
AluI AGCT 9 cut(s) 91, 227, 496, 1049, 1062, 1205, 1279, 1340, 1487
Alw21I GWGCWC 2 cut(s) 229, 912
Alw26I GTCTC 2 cut(s) 193, 612
AlwI GGATC 7 cut(s) 371, 384, 684, 1067, 1080, 1251, 1264
AlwNI CAGNNNCTG 1 cut(s) 1340
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 2 cut(s) 249, 1383
ApeKI GCWGC 3 cut(s) 1142, 1232, 1436
AseI ATTAAT 1 cut(s) 441
AspLEI GCGC 2 cut(s) 107, 470
AspS9I GGNCC 3 cut(s) 249, 349, 1490
AsuHPI GGTGA 4 cut(s) 191, 542, 542, 1111
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 800
AvaII GGWCC 2 cut(s) 349, 1490
BaeGI GKGCMC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 1385
BamHI GGATCC 3 cut(s) 376, 1072, 1256
BanI GGYRCC 3 cut(s) 22, 104, 1012
BanII GRGCYC 1 cut(s) 229
BbsI GAAGAC 2 cut(s) 426, 783
Bbv12I GWGCWC 2 cut(s) 229, 912
BbvI GCAGC 3 cut(s) 1129, 1244, 1423
BccI CCATC 7 cut(s) 140, 266, 311, 521, 998, 1024, 1106
BciT130I CCWGG 2 cut(s) 246, 1321
BciVI GTATCC 1 cut(s) 1414
BclI TGATCA 2 cut(s) 181, 982
BcoDI GTCTC 2 cut(s) 193, 612
BfaI CTAG 2 cut(s) 840, 1106
BfmI CTRYAG 1 cut(s) 1230
BfoI RGCGCY 1 cut(s) 108
BfuI GTATCC 1 cut(s) 1414
BisI GCNGC 3 cut(s) 1143, 1233, 1437
BlpI GCTNAGC 1 cut(s) 1063
BlsI GCNGC 3 cut(s) 1144, 1234, 1438
Bme1390I CCNGG 2 cut(s) 246, 1321
Bme18I GGWCC 2 cut(s) 349, 1490
BmeT110I CYCGRG 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 249, 349, 1490
BmiI GGNNCC 9 cut(s) 24, 48, 106, 250, 350, 378, 1014, 1074, 1258
BmrFI CCNGG 2 cut(s) 246, 1321
BmsI GCATC 3 cut(s) 115, 442, 1423
BpiI GAAGAC 2 cut(s) 426, 783
BplI GAGNNNNNCTC 2 cut(s) 1168, 1200
Bpu1102I GCTNAGC 1 cut(s) 1063
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 518
BsaBI GATNNNNATC 2 cut(s) 375, 381
BsaHI GRCGYC 2 cut(s) 105, 558
BsaJI CCNNGG 3 cut(s) 108, 245, 903
BsaWI WCCGGW 1 cut(s) 884
BsaXI ACNNNNNCTCC 2 cut(s) 1138, 1168
Bsc4I CCNNNNNNNGG 7 cut(s) 175, 176, 246, 478, 611, 1334, 1499
Bse118I RCCGGY 2 cut(s) 43, 101
Bse1I ACTGG 2 cut(s) 1258, 1413
Bse3DI GCAATG 1 cut(s) 481
Bse8I GATNNNNATC 2 cut(s) 375, 381
BseBI CCWGG 2 cut(s) 246, 1321
BseDI CCNNGG 3 cut(s) 108, 245, 903
BseGI GGATG 8 cut(s) 163, 319, 406, 487, 561, 658, 880, 1316
BseJI GATNNNNATC 2 cut(s) 375, 381
BseLI CCNNNNNNNGG 7 cut(s) 175, 176, 246, 478, 611, 1334, 1499
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 3 cut(s) 720, 982, 1074
BseNI ACTGG 2 cut(s) 1258, 1413
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 3 cut(s) 1129, 1244, 1423
BshFI GGCC 2 cut(s) 251, 1385
BshNI GGYRCC 3 cut(s) 22, 104, 1012
BsiHKAI GWGCWC 2 cut(s) 229, 912
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 3 cut(s) 44, 102, 885
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 7 cut(s) 175, 176, 246, 478, 611, 1334, 1499
BsmAI GTCTC 2 cut(s) 193, 612
BsmFI GGGAC 1 cut(s) 362
BsmI GAATGC 1 cut(s) 1087
BsnI GGCC 2 cut(s) 251, 1385
BsoBI CYCGRG 1 cut(s) 800
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 3 cut(s) 27, 229, 912
Bsp143I GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
Bsp1720I GCTNAGC 1 cut(s) 1063
Bsp19I CCATGG 1 cut(s) 903
BspACI CCGC 5 cut(s) 65, 472, 956, 1361, 1439
BspANI GGCC 2 cut(s) 251, 1385
BspCNI CTCAG 3 cut(s) 721, 981, 1075
BspHI TCATGA 1 cut(s) 1195
BspLI GGNNCC 9 cut(s) 24, 48, 106, 250, 350, 378, 1014, 1074, 1258
BspMAI CTGCAG 1 cut(s) 1234
BspPI GGATC 7 cut(s) 371, 384, 684, 1067, 1080, 1251, 1264
BspQI GCTCTTC 3 cut(s) 81, 217, 1466
BspT104I TTCGAA 1 cut(s) 38
BspT107I GGYRCC 3 cut(s) 22, 104, 1012
BsrDI GCAATG 1 cut(s) 481
BsrFI RCCGGY 2 cut(s) 43, 101
BsrI ACTGG 2 cut(s) 1258, 1413
BssAI RCCGGY 2 cut(s) 43, 101
BssECI CCNNGG 3 cut(s) 108, 245, 903
BssMI GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
BssNI GRCGYC 2 cut(s) 105, 558
BssT1I CCWWGG 2 cut(s) 108, 903
Bst2UI CCWGG 2 cut(s) 246, 1321
Bst4CI ACNGT 4 cut(s) 425, 782, 991, 1042
Bst6I CTCTTC 4 cut(s) 81, 217, 1191, 1466
BstACI GRCGYC 2 cut(s) 105, 558
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 4 cut(s) 472, 494, 807, 1383
BstDEI CTNAG 4 cut(s) 729, 968, 1063, 1083
BstDSI CCRYGG 1 cut(s) 903
BstENI CCTNNNNNAGG 2 cut(s) 609, 1332
BstF5I GGATG 8 cut(s) 163, 319, 406, 487, 561, 658, 880, 1316
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 2 cut(s) 107, 470
BstKTI GATC 6 cut(s) 184, 379, 679, 985, 1075, 1259
BstMAI GTCTC 2 cut(s) 193, 612
BstMBI GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
BstMWI GCNNNNNNNGC 2 cut(s) 1151, 1442
BstNI CCWGG 2 cut(s) 246, 1321
BstSCI CCNGG 2 cut(s) 244, 1319
BstSFI CTRYAG 1 cut(s) 1230
BstSLI GKGCMC 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 1129, 1244, 1423
BstV2I GAAGAC 2 cut(s) 426, 783
BstX2I RGATCY 4 cut(s) 376, 676, 1072, 1256
BstXI CCANNNNNNTGG 1 cut(s) 1454
BstYI RGATCY 4 cut(s) 376, 676, 1072, 1256
BsuI GTATCC 1 cut(s) 1414
BsuRI GGCC 2 cut(s) 251, 1385
BtgI CCRYGG 1 cut(s) 903
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 8 cut(s) 163, 319, 406, 487, 561, 658, 880, 1316
BtsIMutI CAGTG 3 cut(s) 430, 976, 1080
Cac8I GCNNGC 4 cut(s) 472, 494, 807, 1383
CaiI CAGNNNCTG 1 cut(s) 1340
CciI TCATGA 1 cut(s) 1195
CfoI GCGC 2 cut(s) 107, 470
Cfr10I RCCGGY 2 cut(s) 43, 101
Cfr13I GGNCC 3 cut(s) 249, 349, 1490
Csp6I GTAC 1 cut(s) 823
CviQI GTAC 1 cut(s) 823
DdeI CTNAG 4 cut(s) 729, 968, 1063, 1083
DinI GGCGCC 1 cut(s) 106
DpnI GATC 6 cut(s) 183, 378, 678, 984, 1074, 1258
DpnII GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 4 cut(s) 81, 217, 1191, 1466
EarI CTCTTC 4 cut(s) 81, 217, 1191, 1466
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 108, 903
Eco24I GRGCYC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 349, 1490
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 800
EcoICRI GAGCTC 1 cut(s) 227
EcoNI CCTNNNNNAGG 2 cut(s) 609, 1332
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 2 cut(s) 244, 1319
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 2 cut(s) 108, 903
EcoT22I ATGCAT 1 cut(s) 1313
EcoT38I GRGCYC 1 cut(s) 229
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
ErhI CCWWGG 2 cut(s) 108, 903
FaqI GGGAC 1 cut(s) 362
FauI CCCGC 3 cut(s) 72, 479, 963
FauNDI CATATG 1 cut(s) 1429
FbaI TGATCA 2 cut(s) 181, 982
Fnu4HI GCNGC 3 cut(s) 1143, 1233, 1437
FokI GGATG 8 cut(s) 170, 326, 413, 494, 548, 665, 887, 1303
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 3 cut(s) 1143, 1233, 1437
FspBI CTAG 2 cut(s) 840, 1106
GlaI GCGC 2 cut(s) 106, 469
GluI GCNGC 3 cut(s) 1143, 1233, 1437
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 2 cut(s) 251, 1385
HapII CCGG 3 cut(s) 44, 102, 885
HhaI GCGC 2 cut(s) 107, 470
Hin1I GRCGYC 2 cut(s) 105, 558
Hin6I GCGC 2 cut(s) 105, 468
HinP1I GCGC 2 cut(s) 105, 468
HindIII AAGCTT 2 cut(s) 1060, 1277
HinfI GANTC 6 cut(s) 413, 518, 791, 943, 1166, 1324
HpaII CCGG 3 cut(s) 44, 102, 885
HphI GGTGA 4 cut(s) 191, 542, 542, 1111
Hpy166II GTNNAC 2 cut(s) 550, 1038
Hpy188I TCNGA 4 cut(s) 221, 418, 523, 1165
Hpy188III TCNNGA 3 cut(s) 680, 997, 1196
Hpy8I GTNNAC 2 cut(s) 550, 1038
Hpy99I CGWCG 2 cut(s) 59, 311
HpyAV CCTTC 5 cut(s) 385, 669, 748, 1314, 1503
HpyCH4III ACNGT 4 cut(s) 425, 782, 991, 1042
HpyCH4IV ACGT 2 cut(s) 327, 558
HpyCH4V TGCA 8 cut(s) 455, 585, 700, 845, 938, 1232, 1250, 1311
HpyF10VI GCNNNNNNNGC 2 cut(s) 1151, 1442
HpyF3I CTNAG 4 cut(s) 729, 968, 1063, 1083
HpySE526I ACGT 2 cut(s) 327, 558
Hsp92I GRCGYC 2 cut(s) 105, 558
HspAI GCGC 2 cut(s) 105, 468
KasI GGCGCC 1 cut(s) 104
Ksp22I TGATCA 2 cut(s) 181, 982
Kzo9I GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
LguI GCTCTTC 3 cut(s) 81, 217, 1466
LmnI GCTCC 4 cut(s) 232, 907, 1159, 1337
Lsp1109I GCAGC 3 cut(s) 1129, 1244, 1423
LweI GCATC 3 cut(s) 115, 442, 1423
MaeI CTAG 2 cut(s) 840, 1106
MaeII ACGT 2 cut(s) 327, 558
MaeIII GTNAC 5 cut(s) 235, 365, 782, 1042, 1460
MalI GATC 6 cut(s) 183, 378, 678, 984, 1074, 1258
MboI GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
MflI RGATCY 4 cut(s) 376, 676, 1072, 1256
MhlI GDGCHC 3 cut(s) 27, 229, 912
MlsI TGGCCA 1 cut(s) 1385
MluCI AATT 2 cut(s) 12, 438
MluNI TGGCCA 1 cut(s) 1385
Mly113I GGCGCC 1 cut(s) 105
MlyI GAGTC 1 cut(s) 1175
Mox20I TGGCCA 1 cut(s) 1385
Mph1103I ATGCAT 1 cut(s) 1313
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 5 cut(s) 96, 441, 647, 864, 1504
MslI CAYNNNNRTG 2 cut(s) 326, 1194
Msp20I TGGCCA 1 cut(s) 1385
MspA1I CMGCKG 1 cut(s) 1049
MspI CCGG 3 cut(s) 44, 102, 885
MspR9I CCNGG 2 cut(s) 246, 1321
Mva1269I GAATGC 1 cut(s) 1087
MvaI CCWGG 2 cut(s) 246, 1321
MwoI GCNNNNNNNGC 2 cut(s) 1151, 1442
NarI GGCGCC 1 cut(s) 105
NcoI CCATGG 1 cut(s) 903
NdeI CATATG 1 cut(s) 1429
NdeII GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
NlaIV GGNNCC 9 cut(s) 24, 48, 106, 250, 350, 378, 1014, 1074, 1258
NmuCI GTSAC 2 cut(s) 782, 1042
NsiI ATGCAT 1 cut(s) 1313
NspV TTCGAA 1 cut(s) 38
PagI TCATGA 1 cut(s) 1195
PciSI GCTCTTC 3 cut(s) 81, 217, 1466
PctI GAATGC 1 cut(s) 1087
PfeI GAWTC 5 cut(s) 413, 518, 791, 943, 1324
PfoI TCCNGGA 1 cut(s) 1319
PkrI GCNGC 3 cut(s) 1144, 1234, 1438
PleI GAGTC 1 cut(s) 1174
PluTI GGCGCC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 1174
PpuMI RGGWCCY 1 cut(s) 349
PshBI ATTAAT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 244, 1319
PspGI CCWGG 2 cut(s) 244, 1319
PspN4I GGNNCC 9 cut(s) 24, 48, 106, 250, 350, 378, 1014, 1074, 1258
PspPI GGNCC 3 cut(s) 249, 349, 1490
PspPPI RGGWCCY 1 cut(s) 349
PstI CTGCAG 1 cut(s) 1234
PstNI CAGNNNCTG 1 cut(s) 1340
PsuI RGATCY 4 cut(s) 376, 676, 1072, 1256
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 1 cut(s) 824
RsaNI GTAC 1 cut(s) 823
RseI CAYNNNNRTG 2 cut(s) 326, 1194
SacI GAGCTC 1 cut(s) 229
SapI GCTCTTC 3 cut(s) 81, 217, 1466
SaqAI TTAA 5 cut(s) 96, 441, 647, 864, 1504
SatI GCNGC 3 cut(s) 1143, 1233, 1437
Sau3AI GATC 6 cut(s) 181, 376, 676, 982, 1072, 1256
Sau96I GGNCC 3 cut(s) 249, 349, 1490
SchI GAGTC 1 cut(s) 1175
ScrFI CCNGG 2 cut(s) 246, 1321
SduI GDGCHC 3 cut(s) 27, 229, 912
SfaNI GCATC 3 cut(s) 115, 442, 1423
SfcI CTRYAG 1 cut(s) 1230
SfoI GGCGCC 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 2 cut(s) 349, 1490
SmiMI CAYNNNNRTG 2 cut(s) 326, 1194
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 2 cut(s) 12, 438
SsiI CCGC 5 cut(s) 65, 472, 956, 1361, 1439
SspDI GGCGCC 1 cut(s) 104
SspI AATATT 1 cut(s) 898
SspMI CTAG 2 cut(s) 840, 1106
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 2 cut(s) 244, 1319
StyI CCWWGG 2 cut(s) 108, 903
TaaI ACNGT 4 cut(s) 425, 782, 991, 1042
TaiI ACGT 2 cut(s) 330, 561
TaqI TCGA 2 cut(s) 38, 84
TasI AATT 2 cut(s) 12, 438
TatI WGTACW 1 cut(s) 822
TfiI GAWTC 5 cut(s) 413, 518, 791, 943, 1324
Tru1I TTAA 5 cut(s) 96, 441, 647, 864, 1504
Tru9I TTAA 5 cut(s) 96, 441, 647, 864, 1504
TscAI CASTG 3 cut(s) 430, 976, 1087
TseFI GTSAC 2 cut(s) 782, 1042
TseI GCWGC 3 cut(s) 1142, 1232, 1436
Tsp45I GTSAC 2 cut(s) 782, 1042
TspDTI ATGAA 9 cut(s) 498, 558, 669, 708, 1080, 1178, 1296, 1305, 1485
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 3 cut(s) 430, 976, 1087
VpaK11BI GGWCC 2 cut(s) 349, 1490
VspI ATTAAT 1 cut(s) 441
XagI CCTNNNNNAGG 2 cut(s) 609, 1332
XcmI CCANNNNNNNNNTGG 1 cut(s) 1267
XspI CTAG 2 cut(s) 840, 1106
ZraI GACGTC 1 cut(s) 559
Zsp2I ATGCAT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.