Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Reverse (-)

16972569 .. 16978161

Gene Structure 5593 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G35830.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2697 bp

ATGGCTTCCGAGAATCCTTTCCGAAGCATATTGAAGGCGTTAGAGAAGCCTGATGGTGGTGAATTCGGTAACTACTACAGCTTACCTGCTTTGAACGATCCCAGGATCGATAAACTACCTTATTCCATTAGGATACTTCTTGAATCGGCCATACGTAACTGTGATGAGTTCCAAGTTAAGAGCAAAGATGTTGAGAAGATTCTTGATTGGGAGAATACTTCTCCCAAGCAGGTTGAGATTCCGTTCAAGCCTGCTCGGGTTCTTCTTCAGGACTTTACTGGTGTTCCTGCTGTTGTTGATCTTGCTTGCATGAGAGATGCCATGAATAATCTCGGTGGTGATTCTAATAAAATTAATCCGCTGGTCCCTGTAGATCTTGTCATTGATCACTCCGTTCAGGTGGATGTGGCGAGATCAGAGAACGCAGTGCAGGCAAACATGGAGCTTGAGTTCCAGCGTAACAAGGAAAGATTTGCTTTTCTTAAGTGGGGATCCAACGCCTTTCACAACATGCTTGTCGTACCTCCTGGATCTGGAATAGTTCATCAAGTCAACCTAGAATACCTTGCCAGAGTTGTTTTCAACACAAATGGACTTCTTTACCCAGACAGTGTTGTTGGCACAGACTCTCACACCACTATGATTGATGGACTGGGTGTTGCTGGATGGGGAGTTGGCGGTATAGAAGCGGAAGCTACCATGCTTGGTCAGCCAATGAGCATGGTCCTACCCGGTGTTGTGGGTTTCAAGCTAACGGGAAAGTTAAGAGATGGAATGACAGCTACTGATTTGGTCTTAACAGTGACTCAGATGTTGAGGAAACATGGAGTAGTTGGAAAGTTTGTTGAATTCCACGGGGAAGGGATGAGAGAATTGTCTTTAGCTGACCGTGCTACAATTGCCAATATGTCTCCTGAGTACGGTGCGACCATGGGATTCTTCCCAGTCGATCATGTCACTTTGCAGTATCTAAGGTTGACAGGCAGGAGCGATGACACTGTCTCCATGATAGAGGCGTATTTACGAGCAAACAAGATGTTTGTGGATTACAGTGAGCCGGAGAGTAAGACAGTTTATTCCTCATGTCTGGAATTGAATCTCGAGGATGTGGAACCTTGTGTTTCTGGTCCCAAGAGGCCTCATGATCGTGTTCCTTTGAAGGAAATGAAAGCGGACTGGCATTCTTGCTTGGACAATAGAGTAGGATTCAAGGGTTTCGCTGTACCTAAAGAAGCACAGAGTAAGGCTGTAGAGTTCAATTTTAACGGGACCACAGCACAGCTTAGACATGGAGATGTTGTTATAGCAGCAATCACCAGTTGCACAAATACTTCAAACCCTAGTGTAATGCTTGGCGCTGCCTTAGTTGCAAAAAAGGCCTGCGACCTAGGACTGGAGGTTAAGCCATGGATCAAAACTAGTCTTGCTCCAGGCTCTGGAGTTGTAACAAAGTACTTGGCAAAGAGTGGCTTGCAGAAGTACTTGAATCAGCTCGGCTTCAGTATCGTTGGTTATGGGTGCACCACATGCATTGGAAACTCGGGGGATATCCATGAAGCTGTGGCTTCAGCAATAGTTGATAATGACTTGGTGGCATCCGCTGTGTTGTCTGGGAACAGAAATTTTGAGGGACGTGTTCACCCGTTAACAAGAGCTAACTATCTAGCTTCCCCACCGCTTGTTGTAGCCTATGCTCTGGCTGGAACTGTTGACATTGATTTTGAGACACAGCCCATTGGAACTGGGAAAGATGGAAAACAGATATTTTTCAGGGACATTTGGCCCTCTAACAAAGAAGTTGCTGAGGTTGTTCAATCTAGTGTCCTTCCTGATATGTTCAAAGCTACATATGAAGCAATCACCAAAGGAAATTCCATGTGGAATCAGTTATCTGTGGCGTCAGGTACTCTCTATGAGTGGGACCCGAAATCAACTTACATTCACGAGCCGCCTTATTTCAAGGGCATGACCATGTCTCCACCCGGTCCACATGGTGTGAAAGACGCATACTGTTTACTCAATTTTGGAGACAGTATTACCACTGATCACATCTCACCAGCTGGTAGCATCCACAAGGACAGTCCTGCGGCTAAGTACTTGATGGAACGAGGTGTGGATAGAAGAGACTTCAACTCATACGGGAGTCGCCGTGGTAATGATGAGATTATGGCGAGAGGCACTTTTGCAAATATCCGTATTGTCAACAAACACTTGAAAGGAGAAGTTGGTCCCAAAACAGTTCACATTCCCACTGGAGAGAAGCTTTCTGTTTTCGATGCTGCCATGAAATATAGGAACGAGGGACGCGACACAATCATTTTGGCTGGTGCTGAATACGGTAGTGGAAGTTCTCGTGATTGGGCTGCCAAGGGTCCAATGCTTCTGGGTGTGAAAGCTGTGATTTCAAAGAGCTTCGAGCGAATTCACCGAAGCAATTTGGTGGGAATGGGAATCATACCTTTGTGCTTCAAGGCGGGAGAAGATGCTGAGACCCTTGGCCTAACGGGTCAGGAGCTTTACACCATTGAGCTCCCAAACAATGTTAGTGAGATCAAACCAGGACAAGATGTAACAGTCGTCACAAACAATGGCAAATCTTTCACATGTACACTCCGATTTGACACAGAGGTGGAGTTGGCTTATTTCGATCACGGAGGGATTTTGCAATACGTTATCAGGAACTTGATCAAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

898

Amino Acids

98.15

Weight (kDa)

5.98

Isoelectric Point (pI)

33.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	1 - 465	2.4e-159	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	594 - 722	3.1e-40	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	94, 220
AccB7I	CCANNNNNTGG	1 cut(s)	1436
AccII	CGCG	1 cut(s)	2307
AciI	CCGC	9 cut(s)	359, 678, 689, 1172, 1599, 1676, 1949, 2087, 2474
AclWI	GGATC	6 cut(s)	92, 113, 486, 499, 538, 1418
AcoI	YGGCCR	1 cut(s)	147
AcsI	RAATTY	5 cut(s)	62, 848, 1621, 1870, 2421
AcuI	CTGAAG	3 cut(s)	251, 1483, 1551
AcyI	GRCGYC	1 cut(s)	1898
AdeI	CACNNNGTG	1 cut(s)	1994
AfaI	GTAC	8 cut(s)	522, 920, 1224, 1454, 1481, 1906, 2096, 2608
AfiI	CCNNNNNNNGG	5 cut(s)	56, 533, 920, 1393, 1436
AflII	CTTAAG	1 cut(s)	482
AflIII	ACRYGT	2 cut(s)	1633, 2603
AhlI	ACTAGT	1 cut(s)	1418
AjiI	CACGTC	1 cut(s)	1634
AjnI	CCWGG	4 cut(s)	101, 526, 1429, 2557
AleI	CACNNNNGTG	1 cut(s)	2627
AloI	GAACNNNNNNTCC	2 cut(s)	434, 466
Alw21I	GWGCWC	2 cut(s)	1523, 2532
Alw26I	GTCTC	7 cut(s)	915, 1006, 1718, 1980, 2022, 2118, 2483
Alw44I	GTGCAC	1 cut(s)	1519
AlwI	GGATC	6 cut(s)	92, 113, 486, 499, 538, 1418
AlwNI	CAGNNNCTG	2 cut(s)	785, 1436
Ama87I	CYCGRG	3 cut(s)	255, 1100, 1540
AoxI	GGCC	5 cut(s)	147, 1136, 1377, 1781, 2497
ApaLI	GTGCAC	1 cut(s)	1519
ApeKI	GCWGC	4 cut(s)	1307, 1358, 2279, 2363
ApoI	RAATTY	5 cut(s)	62, 848, 1621, 1870, 2421
ArsI	GACNNNNNNTTYG	2 cut(s)	1703, 1735
AseI	ATTAAT	1 cut(s)	354
Asp700I	GAANNNNTTC	1 cut(s)	17
AspA2I	CCTAGG	1 cut(s)	1387
AspLEI	GCGC	1 cut(s)	1358
AspS9I	GGNCC	9 cut(s)	364, 724, 1127, 1269, 1782, 1921, 1985, 2228, 2372
AsuC2I	CCSGG	2 cut(s)	732, 1983
AsuHPI	GGTGA	7 cut(s)	71, 350, 1306, 1631, 1852, 2046, 2417
AvaI	CYCGRG	3 cut(s)	255, 1100, 1540
AvaII	GGWCC	8 cut(s)	364, 724, 1127, 1269, 1921, 1985, 2228, 2372
AvrII	CCTAGG	1 cut(s)	1387
BaeGI	GKGCMC	1 cut(s)	1523
BamHI	GGATCC	1 cut(s)	491
BanII	GRGCYC	1 cut(s)	2532
BauI	CACGAG	2 cut(s)	1943, 2352
Bbv12I	GWGCWC	2 cut(s)	1523, 2532
BbvCI	CCTCAGC	1 cut(s)	1803
BbvI	GCAGC	4 cut(s)	1319, 1345, 2266, 2350
BccI	CCATC	6 cut(s)	47, 641, 660, 764, 1745, 2095
BceAI	ACGGC	1 cut(s)	2133
BciT130I	CCWGG	4 cut(s)	103, 528, 1431, 2559
BciVI	GTATCC	1 cut(s)	126
BclI	TGATCA	3 cut(s)	385, 2044, 2685
BcnI	CCSGG	2 cut(s)	732, 1983
BcoDI	GTCTC	7 cut(s)	915, 1006, 1718, 1980, 2022, 2118, 2483
BcuI	ACTAGT	1 cut(s)	1418
BfaI	CTAG	6 cut(s)	557, 1341, 1388, 1419, 1664, 1818
BfmI	CTRYAG	3 cut(s)	76, 369, 1248
BfoI	RGCGCY	1 cut(s)	1359
BfrI	CTTAAG	1 cut(s)	482
BfuAI	ACCTGC	2 cut(s)	94, 220
BfuI	GTATCC	1 cut(s)	126
BglII	AGATCT	1 cut(s)	373
BisI	GCNGC	6 cut(s)	1308, 1359, 1949, 2088, 2280, 2364
BlnI	CCTAGG	1 cut(s)	1387
BlsI	GCNGC	6 cut(s)	1309, 1360, 1950, 2089, 2281, 2365
BmcAI	AGTACT	3 cut(s)	1454, 1481, 2096
Bme1390I	CCNGG	6 cut(s)	103, 528, 732, 1431, 1983, 2559
Bme18I	GGWCC	8 cut(s)	364, 724, 1127, 1269, 1921, 1985, 2228, 2372
BmeT110I	CYCGRG	3 cut(s)	255, 1100, 1540
BmgBI	CACGTC	1 cut(s)	1634
BmgT120I	GGNCC	9 cut(s)	364, 724, 1127, 1269, 1782, 1921, 1985, 2228, 2372
BmiI	GGNNCC	9 cut(s)	366, 493, 1113, 1129, 1270, 1922, 1923, 2230, 2373
BmrFI	CCNGG	6 cut(s)	103, 528, 732, 1431, 1983, 2559
BmrI	ACTGGG	3 cut(s)	662, 938, 1752
BmsI	GCATC	5 cut(s)	307, 1604, 2076, 2266, 2473
BmuI	ACTGGG	3 cut(s)	662, 938, 1752
BpmI	CTGGAG	4 cut(s)	1413, 1415, 1458, 2274
Bpu10I	CCTNAGC	1 cut(s)	1803
BpuEI	CTTGAG	1 cut(s)	467
BpuMI	CCSGG	2 cut(s)	732, 1983
Bsa29I	ATCGAT	1 cut(s)	108
BsaAI	YACGTR	1 cut(s)	155
BsaHI	GRCGYC	1 cut(s)	1898
BsaI	GGTCTC	1 cut(s)	2483
BsaJI	CCNNGG	8 cut(s)	101, 853, 930, 1387, 1406, 2149, 2367, 2494
Bsc4I	CCNNNNNNNGG	5 cut(s)	56, 533, 920, 1393, 1436
Bse1I	ACTGG	8 cut(s)	283, 657, 944, 1181, 1317, 1398, 1747, 2257
BseBI	CCWGG	4 cut(s)	103, 528, 1431, 2559
BseCI	ATCGAT	1 cut(s)	108
BseDI	CCNNGG	8 cut(s)	101, 853, 930, 1387, 1406, 2149, 2367, 2494
BseGI	GGATG	6 cut(s)	409, 671, 870, 1111, 1595, 2067
BseLI	CCNNNNNNNGG	5 cut(s)	56, 533, 920, 1393, 1436
BseMII	CTCAG	4 cut(s)	821, 906, 1794, 2478
BseNI	ACTGG	8 cut(s)	283, 657, 944, 1181, 1317, 1398, 1747, 2257
BseSI	GKGCMC	1 cut(s)	1523
BseXI	GCAGC	4 cut(s)	1319, 1345, 2266, 2350
BsgI	GTGCAG	1 cut(s)	449
Bsh1236I	CGCG	1 cut(s)	2307
BshFI	GGCC	5 cut(s)	149, 1138, 1379, 1783, 2499
BshVI	ATCGAT	1 cut(s)	108
BsiHKAI	GWGCWC	2 cut(s)	1523, 2532
BsiHKCI	CYCGRG	3 cut(s)	255, 1100, 1540
BsiSI	CCGG	3 cut(s)	732, 1058, 1983
BslFI	GGGAC	8 cut(s)	350, 1113, 1282, 1644, 1787, 1934, 2214, 2316
BslI	CCNNNNNNNGG	5 cut(s)	56, 533, 920, 1393, 1436
BsmAI	GTCTC	7 cut(s)	915, 1006, 1718, 1980, 2022, 2118, 2483
BsmFI	GGGAC	8 cut(s)	350, 1113, 1282, 1644, 1787, 1934, 2214, 2316
BsmI	GAATGC	1 cut(s)	1180
BsnI	GGCC	5 cut(s)	149, 1138, 1379, 1783, 2499
Bso31I	GGTCTC	1 cut(s)	2483
BsoBI	CYCGRG	3 cut(s)	255, 1100, 1540
Bsp1286I	GDGCHC	2 cut(s)	1523, 2532
Bsp1407I	TGTACA	1 cut(s)	2606
Bsp19I	CCATGG	2 cut(s)	930, 1406
BspACI	CCGC	9 cut(s)	359, 678, 689, 1172, 1599, 1676, 1949, 2087, 2474
BspANI	GGCC	5 cut(s)	149, 1138, 1379, 1783, 2499
BspCNI	CTCAG	4 cut(s)	820, 907, 1795, 2479
BspDI	ATCGAT	1 cut(s)	108
BspFNI	CGCG	1 cut(s)	2307
BspHI	TCATGA	1 cut(s)	1141
BspLI	GGNNCC	9 cut(s)	366, 493, 1113, 1129, 1270, 1922, 1923, 2230, 2373
BspMI	ACCTGC	2 cut(s)	94, 220
BspPI	GGATC	6 cut(s)	92, 113, 486, 499, 538, 1418
BspTI	CTTAAG	1 cut(s)	482
BspTNI	GGTCTC	1 cut(s)	2483
BsrGI	TGTACA	1 cut(s)	2606
BsrI	ACTGG	8 cut(s)	283, 657, 944, 1181, 1317, 1398, 1747, 2257
BssECI	CCNNGG	8 cut(s)	101, 853, 930, 1387, 1406, 2149, 2367, 2494
BssNI	GRCGYC	1 cut(s)	1898
BssSI	CACGAG	2 cut(s)	1943, 2352
BssT1I	CCWWGG	5 cut(s)	930, 1387, 1406, 2367, 2494
Bst2BI	CACGAG	2 cut(s)	1943, 2352
Bst2UI	CCWGG	4 cut(s)	103, 528, 1431, 2559
Bst6I	CTCTTC	1 cut(s)	2116
BstACI	GRCGYC	1 cut(s)	1898
BstAFI	CTTAAG	1 cut(s)	482
BstAPI	GCANNNNNTGC	1 cut(s)	1527
BstAUI	TGTACA	1 cut(s)	2606
BstBAI	YACGTR	1 cut(s)	155
BstC8I	GCNNGC	5 cut(s)	252, 307, 432, 1381, 1472
BstDEI	CTNAG	8 cut(s)	807, 915, 971, 1283, 1363, 1803, 2091, 2487
BstDSI	CCRYGG	4 cut(s)	853, 930, 1406, 2149
BstF5I	GGATG	6 cut(s)	409, 671, 870, 1111, 1595, 2067
BstFNI	CGCG	1 cut(s)	2307
BstH2I	RGCGCY	1 cut(s)	1359
BstHHI	GCGC	1 cut(s)	1358
BstMAI	GTCTC	7 cut(s)	915, 1006, 1718, 1980, 2022, 2118, 2483
BstMWI	GCNNNNNNNGC	7 cut(s)	431, 709, 890, 899, 1367, 1376, 1527
BstNI	CCWGG	4 cut(s)	103, 528, 1431, 2559
BstNSI	RCATGY	3 cut(s)	514, 1530, 2607
BstSCI	CCNGG	6 cut(s)	101, 526, 730, 1429, 1981, 2557
BstSFI	CTRYAG	3 cut(s)	76, 369, 1248
BstSLI	GKGCMC	1 cut(s)	1523
BstSNI	TACGTA	1 cut(s)	155
BstUI	CGCG	1 cut(s)	2307
BstV1I	GCAGC	4 cut(s)	1319, 1345, 2266, 2350
BstX2I	RGATCY	3 cut(s)	373, 491, 530
BstYI	RGATCY	3 cut(s)	373, 491, 530
Bsu15I	ATCGAT	1 cut(s)	108
BsuI	GTATCC	1 cut(s)	126
BsuRI	GGCC	5 cut(s)	149, 1138, 1379, 1783, 2499
BsuTUI	ATCGAT	1 cut(s)	108
BtgI	CCRYGG	4 cut(s)	853, 930, 1406, 2149
BtgZI	GCGATG	1 cut(s)	1005
BtrI	CACGTC	1 cut(s)	1634
BtsCI	GGATG	6 cut(s)	409, 671, 870, 1111, 1595, 2067
BtsI	GCAGTG	1 cut(s)	432
BtsIMutI	CAGTG	7 cut(s)	432, 616, 807, 996, 1057, 2040, 2250
BveI	ACCTGC	2 cut(s)	94, 220
Cac8I	GCNNGC	5 cut(s)	252, 307, 432, 1381, 1472
CaiI	CAGNNNCTG	2 cut(s)	785, 1436
CciI	TCATGA	1 cut(s)	1141
CfoI	GCGC	1 cut(s)	1358
Cfr13I	GGNCC	9 cut(s)	364, 724, 1127, 1269, 1782, 1921, 1985, 2228, 2372
ClaI	ATCGAT	1 cut(s)	108
CseI	GACGC	3 cut(s)	1887, 2012, 2313
Csp6I	GTAC	8 cut(s)	521, 919, 1223, 1453, 1480, 1905, 2095, 2607
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 1513, 1548
CviQI	GTAC	8 cut(s)	521, 919, 1223, 1453, 1480, 1905, 2095, 2607
DdeI	CTNAG	8 cut(s)	807, 915, 971, 1283, 1363, 1803, 2091, 2487
DraIII	CACNNNGTG	1 cut(s)	1994
EaeI	YGGCCR	1 cut(s)	147
Eam1104I	CTCTTC	1 cut(s)	2116
EarI	CTCTTC	1 cut(s)	2116
Ecl136II	GAGCTC	1 cut(s)	2530
Eco105I	TACGTA	1 cut(s)	155
Eco130I	CCWWGG	5 cut(s)	930, 1387, 1406, 2367, 2494
Eco147I	AGGCCT	2 cut(s)	1138, 1379
Eco24I	GRGCYC	1 cut(s)	2532
Eco31I	GGTCTC	1 cut(s)	2483
Eco32I	GATATC	1 cut(s)	1549
Eco47I	GGWCC	8 cut(s)	364, 724, 1127, 1269, 1921, 1985, 2228, 2372
Eco53kI	GAGCTC	1 cut(s)	2530
Eco57I	CTGAAG	3 cut(s)	251, 1483, 1551
Eco88I	CYCGRG	3 cut(s)	255, 1100, 1540
EcoICRI	GAGCTC	1 cut(s)	2530
EcoO109I	RGGNCCY	1 cut(s)	1921
EcoRI	GAATTC	3 cut(s)	62, 848, 2421
EcoRII	CCWGG	4 cut(s)	101, 526, 1429, 2557
EcoRV	GATATC	1 cut(s)	1549
EcoT14I	CCWWGG	5 cut(s)	930, 1387, 1406, 2367, 2494
EcoT22I	ATGCAT	1 cut(s)	1532
EcoT38I	GRGCYC	1 cut(s)	2532
ErhI	CCWWGG	5 cut(s)	930, 1387, 1406, 2367, 2494
FalI	AAGNNNNNCTT	4 cut(s)	460, 492, 1454, 1486
FaqI	GGGAC	8 cut(s)	350, 1113, 1282, 1644, 1787, 1934, 2214, 2316
FauI	CCCGC	1 cut(s)	2467
FauNDI	CATATG	1 cut(s)	1849
FbaI	TGATCA	3 cut(s)	385, 2044, 2685
Fnu4HI	GCNGC	6 cut(s)	1308, 1359, 1949, 2088, 2280, 2364
FokI	GGATG	6 cut(s)	416, 678, 877, 1118, 1582, 2054
FriOI	GRGCYC	1 cut(s)	2532
Fsp4HI	GCNGC	6 cut(s)	1308, 1359, 1949, 2088, 2280, 2364
FspBI	CTAG	6 cut(s)	557, 1341, 1388, 1419, 1664, 1818
GlaI	GCGC	1 cut(s)	1357
GluI	GCNGC	6 cut(s)	1308, 1359, 1949, 2088, 2280, 2364
GsuI	CTGGAG	4 cut(s)	1413, 1415, 1458, 2274
HaeII	RGCGCY	1 cut(s)	1359
HaeIII	GGCC	5 cut(s)	149, 1138, 1379, 1783, 2499
HapII	CCGG	3 cut(s)	732, 1058, 1983
HgaI	GACGC	3 cut(s)	1887, 2012, 2313
HhaI	GCGC	1 cut(s)	1358
Hin1I	GRCGYC	1 cut(s)	1898
Hin6I	GCGC	1 cut(s)	1356
HinP1I	GCGC	1 cut(s)	1356
HincII	GTYRAC	5 cut(s)	553, 978, 1647, 1711, 2203
HindII	GTYRAC	5 cut(s)	553, 978, 1647, 1711, 2203
HindIII	AAGCTT	1 cut(s)	2261
HpaI	GTTAAC	1 cut(s)	1647
HpaII	CCGG	3 cut(s)	732, 1058, 1983
HphI	GGTGA	7 cut(s)	71, 350, 1306, 1631, 1852, 2046, 2417
Hpy188I	TCNGA	5 cut(s)	10, 23, 418, 810, 2615
HpyAV	CCTTC	4 cut(s)	28, 854, 1153, 1835
HpyCH4IV	ACGT	3 cut(s)	154, 1633, 2670
HpyF10VI	GCNNNNNNNGC	7 cut(s)	431, 709, 890, 899, 1367, 1376, 1527
HpyF3I	CTNAG	8 cut(s)	807, 915, 971, 1283, 1363, 1803, 2091, 2487
HpySE526I	ACGT	3 cut(s)	154, 1633, 2670
Hsp92I	GRCGYC	1 cut(s)	1898
HspAI	GCGC	1 cut(s)	1356
KflI	GGGWCCC	1 cut(s)	1921
Ksp22I	TGATCA	3 cut(s)	385, 2044, 2685
KspAI	GTTAAC	1 cut(s)	1647
LmnI	GCTCC	5 cut(s)	442, 987, 1432, 2512, 2535
Lsp1109I	GCAGC	4 cut(s)	1319, 1345, 2266, 2350
LweI	GCATC	5 cut(s)	307, 1604, 2076, 2266, 2473
MaeI	CTAG	6 cut(s)	557, 1341, 1388, 1419, 1664, 1818
MaeII	ACGT	3 cut(s)	154, 1633, 2670
MaeIII	GTNAC	8 cut(s)	68, 155, 458, 802, 955, 1444, 2569, 2578
MboII	GAAGA	6 cut(s)	208, 254, 257, 931, 2133, 2492
MfeI	CAATTG	1 cut(s)	897
MflI	RGATCY	3 cut(s)	373, 491, 530
MhlI	GDGCHC	2 cut(s)	1523, 2532
MlyI	GAGTC	3 cut(s)	620, 799, 2152
MmeI	TCCRAC	2 cut(s)	519, 814
Mph1103I	ATGCAT	1 cut(s)	1532
MroXI	GAANNNNTTC	1 cut(s)	17
MseI	TTAA	8 cut(s)	177, 354, 483, 764, 797, 1263, 1401, 1646
MslI	CAYNNNNRTG	5 cut(s)	638, 1146, 1293, 1970, 2627
MspA1I	CMGCKG	3 cut(s)	361, 1601, 2060
MspCI	CTTAAG	1 cut(s)	482
MspI	CCGG	3 cut(s)	732, 1058, 1983
MspR9I	CCNGG	6 cut(s)	103, 528, 732, 1431, 1983, 2559
MunI	CAATTG	1 cut(s)	897
Mva1269I	GAATGC	1 cut(s)	1180
MvaI	CCWGG	4 cut(s)	103, 528, 1431, 2559
MvnI	CGCG	1 cut(s)	2307
MwoI	GCNNNNNNNGC	7 cut(s)	431, 709, 890, 899, 1367, 1376, 1527
NciI	CCSGG	2 cut(s)	732, 1983
NcoI	CCATGG	2 cut(s)	930, 1406
NdeI	CATATG	1 cut(s)	1849
NlaIV	GGNNCC	9 cut(s)	366, 493, 1113, 1129, 1270, 1922, 1923, 2230, 2373
NmeAIII	GCCGAG	1 cut(s)	1473
NmuCI	GTSAC	3 cut(s)	802, 955, 2578
NsiI	ATGCAT	1 cut(s)	1532
NspI	RCATGY	3 cut(s)	514, 1530, 2607
OliI	CACNNNNGTG	1 cut(s)	2627
PaeR7I	CTCGAG	1 cut(s)	1100
PagI	TCATGA	1 cut(s)	1141
PceI	AGGCCT	2 cut(s)	1138, 1379
PciI	ACATGT	1 cut(s)	2603
PcsI	WCGNNNNNNNCGW	1 cut(s)	2304
PctI	GAATGC	1 cut(s)	1180
PdmI	GAANNNNTTC	1 cut(s)	17
PflFI	GACNNNGTC	2 cut(s)	998, 1972
PflMI	CCANNNNNTGG	1 cut(s)	1436
PfoI	TCCNGGA	1 cut(s)	526
PkrI	GCNGC	6 cut(s)	1309, 1360, 1950, 2089, 2281, 2365
PleI	GAGTC	3 cut(s)	620, 799, 2151
PpsI	GAGTC	3 cut(s)	620, 799, 2151
Ppu21I	YACGTR	1 cut(s)	155
PpuMI	RGGWCCY	1 cut(s)	1921
PscI	ACATGT	1 cut(s)	2603
PshBI	ATTAAT	1 cut(s)	354
Psp124BI	GAGCTC	1 cut(s)	2532
Psp5II	RGGWCCY	1 cut(s)	1921
Psp6I	CCWGG	4 cut(s)	101, 526, 1429, 2557
PspGI	CCWGG	4 cut(s)	101, 526, 1429, 2557
PspN4I	GGNNCC	9 cut(s)	366, 493, 1113, 1129, 1270, 1922, 1923, 2230, 2373
PspPI	GGNCC	9 cut(s)	364, 724, 1127, 1269, 1782, 1921, 1985, 2228, 2372
PspPPI	RGGWCCY	1 cut(s)	1921
PsrI	GAACNNNNNNTAC	2 cut(s)	2332, 2364
PstNI	CAGNNNCTG	2 cut(s)	785, 1436
PsuI	RGATCY	3 cut(s)	373, 491, 530
PsyI	GACNNNGTC	2 cut(s)	998, 1972
PvuII	CAGCTG	1 cut(s)	2060
RsaI	GTAC	8 cut(s)	522, 920, 1224, 1454, 1481, 1906, 2096, 2608
RsaNI	GTAC	8 cut(s)	521, 919, 1223, 1453, 1480, 1905, 2095, 2607
RseI	CAYNNNNRTG	5 cut(s)	638, 1146, 1293, 1970, 2627
SacI	GAGCTC	1 cut(s)	2532
SaqAI	TTAA	8 cut(s)	177, 354, 483, 764, 797, 1263, 1401, 1646
SatI	GCNGC	6 cut(s)	1308, 1359, 1949, 2088, 2280, 2364
Sau96I	GGNCC	9 cut(s)	364, 724, 1127, 1269, 1782, 1921, 1985, 2228, 2372
ScaI	AGTACT	3 cut(s)	1454, 1481, 2096
SchI	GAGTC	3 cut(s)	620, 799, 2152
ScrFI	CCNGG	6 cut(s)	103, 528, 732, 1431, 1983, 2559
SduI	GDGCHC	2 cut(s)	1523, 2532
SfaNI	GCATC	5 cut(s)	307, 1604, 2076, 2266, 2473
SfcI	CTRYAG	3 cut(s)	76, 369, 1248
Sfr274I	CTCGAG	1 cut(s)	1100
SinI	GGWCC	8 cut(s)	364, 724, 1127, 1269, 1921, 1985, 2228, 2372
SlaI	CTCGAG	1 cut(s)	1100
SmiMI	CAYNNNNRTG	5 cut(s)	638, 1146, 1293, 1970, 2627
SmlI	CTYRAG	3 cut(s)	446, 482, 1100
SmoI	CTYRAG	3 cut(s)	446, 482, 1100
SnaBI	TACGTA	1 cut(s)	155
SpeI	ACTAGT	1 cut(s)	1418
SseBI	AGGCCT	2 cut(s)	1138, 1379
SsiI	CCGC	9 cut(s)	359, 678, 689, 1172, 1599, 1676, 1949, 2087, 2474
SspMI	CTAG	6 cut(s)	557, 1341, 1388, 1419, 1664, 1818
SstI	GAGCTC	1 cut(s)	2532
StuI	AGGCCT	2 cut(s)	1138, 1379
StyD4I	CCNGG	6 cut(s)	101, 526, 730, 1429, 1981, 2557
StyI	CCWWGG	5 cut(s)	930, 1387, 1406, 2367, 2494
TaiI	ACGT	3 cut(s)	157, 1636, 2673
TaqI	TCGA	6 cut(s)	108, 948, 1101, 2274, 2415, 2646
TatI	WGTACW	4 cut(s)	1452, 1479, 2094, 2606
TauI	GCSGC	2 cut(s)	1951, 2090
Tru1I	TTAA	8 cut(s)	177, 354, 483, 764, 797, 1263, 1401, 1646
Tru9I	TTAA	8 cut(s)	177, 354, 483, 764, 797, 1263, 1401, 1646
TscAI	CASTG	7 cut(s)	432, 616, 807, 1003, 1057, 2047, 2257
TseFI	GTSAC	3 cut(s)	802, 955, 2578
TseI	GCWGC	4 cut(s)	1307, 1358, 2279, 2363
Tsp45I	GTSAC	3 cut(s)	802, 955, 2578
TspDTI	ATGAA	6 cut(s)	338, 533, 1181, 1569, 1866, 2300
TspGWI	ACGGA	4 cut(s)	231, 382, 2183, 2667
TspRI	CASTG	7 cut(s)	432, 616, 807, 1003, 1057, 2047, 2257
Tth111I	GACNNNGTC	2 cut(s)	998, 1972
Van91I	CCANNNNNTGG	1 cut(s)	1436
Vha464I	CTTAAG	1 cut(s)	482
VneI	GTGCAC	1 cut(s)	1519
VpaK11BI	GGWCC	8 cut(s)	364, 724, 1127, 1269, 1921, 1985, 2228, 2372
VspI	ATTAAT	1 cut(s)	354
XapI	RAATTY	5 cut(s)	62, 848, 1621, 1870, 2421
XceI	RCATGY	3 cut(s)	514, 1530, 2607
XhoI	CTCGAG	1 cut(s)	1100
XmaJI	CCTAGG	1 cut(s)	1387
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	6 cut(s)	557, 1341, 1388, 1419, 1664, 1818
ZrmI	AGTACT	3 cut(s)	1454, 1481, 2096
Zsp2I	ATGCAT	1 cut(s)	1532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.