Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Forward (+)

81946679 .. 81952779

Gene Structure 6101 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00144020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGCGGCGGAGAATCCGTTCAGGAGTATATTGAAATCGCTTGAGAAGCCGGACGGCGGCGAGTTCGGAAAGTACTACAGCTTGCCGGCTCTCAATGATCCTAGAGTCGATAAGCTACCGTACTCTATTAAGATCCTTCTCGAGTCTGCAATCCGTAACTGCGATGAGTTTCAGGTTAAGAGTAAGGATGTTGAGAAAATTATTGATTGGGAGAACAGTGCTCCCAAGCAGGTTGAGATTCCGTTCAAACCTGCTAGAGTCCTTCTTCAGGATTTCACTGGTGTACCTGCTGTTGTTGATCTGGCTTGCATGCGAGATGCCATGAACAATCTCAAGGGAGATTCTAACAAGATTAACCCATTGGTACCAGTTGATCTCGTCATTGATCACTCGGTTCAAGTAGATGTGGCAAGATCAGACAATGCAGTGCAGGCAAACATGGAATTTGAGTTCAAGAGAAACAATGAACGGTTTGGGTTTCTAAAATGGGGATCAAATGCATTCCACAACATGCTCGTTGTTCCTCCTGGATCAGGGATTGTTCATCAGGTTAATTTAGAATACCTTGGACGGGTGGTGTTCAACTCAGATGGCTTGCTATATCCTGATAGTGTTGTTGGGACAGATTCACACACCACAATGATTGACGGACTAGGTGTTGCTGGTTGGGGAGTGGGTGGGATCGAAGCAGAAGCTGCGATGCTTGGCCAGCCCATGAGCATGGTCCTCCCTGGTGTGGTTGGGTTCAAGTTGTTAGGAAAACTGAGAGATGGTGTGACAGCTACTGATTTGGTTCTGACAGTAACTCAAATGCTGAGGAAGCATGGAGTTGTTGGGAAGTTTGTGGAGTTTTATGGGGAAGGCATGAGTGAATTGTCATTAGCTGACCGTGCTACTATTGCCAACATGTCTCCTGAGTATGGTGCAACCATGGGCTTCTTTCCTGTCGATCGTGTCACGCTGCAATATCTAAAGCTAACAGGCAGAACTGCTGACACTGTTGCTATGATAGAATCTTATTTACGGGCTAATAAGATGTTCGTGGACTACAGTGAGCCCCAGATAGAGAGAGTATACTCTGCTAATCTTGAGCTAAAACTTGAGGATGTGGAGCCTTGCATATCAGGGCCAAAGAGGCCACATGATCGTGTTTCTCTGAAAGAAATGAAAGTGGATTGGCATGCATGCCTTGACAATAGAGTTGGATTTAAGGGTTTTGCTGTGCCAAAGGAATCTCAAAAGAAAGTTGTAGAGTTCTCATTTCATGGAACCCCTGCACAGCTTAGGCATGGAGATGTCGTAATTGCAGCTATTACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTAGGAGCTGCCTTGGTTGCAAAGAAAGCTTGTGAATTAGGATTGGAGGTTGGATGTCTACTTTTGGCTGTTTCTCATGTTGGTATAATCATATATAGGTTGAGAAAAAGTGAAAAGGAAGTATATGATGCATCAGTCAAGCCATGGATTAAGACTAGTCTTGCGCCAGGCTCTGGGGTTGTAACCAAATACTTGGAAAAGAGTGGGTTGCAGAAGTATCTGAATCAGCTAGGATTTAATATCGTTGGGTATGGATGCACCACATGCATTGGAAATTCAGGAGACATTGATGAAGCAGTGGCATCAGCTATTACGGAAAATGATATTGTAGCTTCAGCCGTATTATCTGGAAATAGGAATTTTGAGGGCCGTGTACATCCTTTAACACGGGCTAATTATCTTGCTTCTCCTCCCCTTGTGGTTGCTTATGCTCTTGCTGGCACGGTGGATATTGATTTTGACACTGAACCTATAGGGGTGGGAAAAGATGGAAAGAAGATATTTTTCAGGGATTTATGGCCATCTAATCAAGAAGTAGCAGAGGCTGTGCAATCAAATGTGCTTCCTCAGATGTTTATGGCTACATATGAAGCAATCACCAAAGGAAATCCCATGTGGAATCAGTTATCTGTACCTTCTGGCACTCTCTATGCATGGGACCCAAAGTCCACTTATATACATGAGCCACCTTATTTTAAAGATATGACCATGTCTCCTCCCGGCCCTCATGGTGTGAAGAATGCTTACTGCTTGCTCAACTTTGGAGATAGTATTACAACTGACCACATCTCACCAGCTGGTAGCATCCACAAAGATAGTCCAGCAGCCAAATACCTTATGGAACGTGGTGTTGATAGAAGAGACTTCAACTCTTATGGTAGTCGTCGTGGTAATGACGAAATAATGGCAAGAGGTACTTTTGCAAATATTCGCCTGGTCAATAAGTTTTTGAAAGGAGAAGTGGGCCCCAAAACAATTCATCATCCCTCAGGAGAGAAAGTTTCCGTGTTTGATGCTGCCATGAAGTACAAAAGTGAAGGGCATGACACAATCATTTTGGCTGGTGCTGAATATGGGAGTGGAAGTTCTCGTGATTGGGCTGCTAAGGGTCCAATGCTACTGGGGGTGAAGGCCGTCATAGCAAAGAGCTTTGAGCGCATTCACCGCAGTAATCTTGTGGGCATGGGTATCATTCCCCTATGCTTCAAGGCTGGGGAGGATGCTGAAACTCTTGGATTGACTGGCGAGGAACGCTACACCATCGACCTTCCAAGCACTGTTAGCGAAATTAGACCCGGTCAGGATATCAGAGTGGTGACAGACAATGGAAAGTCATTTGTATGTACGCTACGGTTTGATACAGAGGTGGAGCTGGCTTATTTTGATCACGGAGGCATTTTGCAATATGTCATCAGGAATCTGATTGGCGCAAAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

929

Amino Acids

101.63

Weight (kDa)

6.11

Isoelectric Point (pI)

33.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	65 - 467	4.3e-125	Aconitase family (aconitate hydratase)
Aconitase	PF00330	493 - 597	1.7e-34	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	726 - 854	5.2e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	220, 259, 295
Acc65I	GGTACC	1 cut(s)	364
AccB1I	GGYRCC	1 cut(s)	364
AccB7I	CCANNNNNTGG	1 cut(s)	1523
AccI	GTMKAC	2 cut(s)	1074, 1408
AciI	CCGC	4 cut(s)	5, 8, 57, 2515
AclWI	GGATC	5 cut(s)	92, 127, 499, 538, 689
AcoI	YGGCCR	2 cut(s)	706, 1868
AcsI	RAATTY	3 cut(s)	443, 1624, 1708
AcuI	CTGAAG	2 cut(s)	251, 1668
AfaI	GTAC	9 cut(s)	74, 122, 285, 366, 1725, 1983, 2266, 2378, 2695
AfiI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1523
AflIII	ACRYGT	1 cut(s)	906
AgsI	TTSAA	9 cut(s)	34, 247, 398, 454, 583, 748, 2218, 2302, 2557
AhdI	GACNNNNNGTC	1 cut(s)	2014
AhlI	ACTAGT	1 cut(s)	1505
AjnI	CCWGG	4 cut(s)	526, 730, 1516, 2283
AloI	GAACNNNNNNTCC	5 cut(s)	34, 434, 466, 2419, 2451
Alw21I	GWGCWC	1 cut(s)	223
Alw26I	GTCTC	4 cut(s)	915, 1626, 2067, 2205
AlwI	GGATC	5 cut(s)	92, 127, 499, 538, 689
AlwNI	CAGNNNCTG	4 cut(s)	695, 785, 1523, 1895
Ama87I	CYCGRG	1 cut(s)	140
AoxI	GGCC	8 cut(s)	706, 1127, 1136, 1717, 1868, 2071, 2314, 2481
ApaI	GGGCCC	1 cut(s)	2318
ApeKI	GCWGC	7 cut(s)	695, 961, 1307, 1358, 2174, 2366, 2450
ApoI	RAATTY	3 cut(s)	443, 1624, 1708
Asp700I	GAANNNNTTC	1 cut(s)	17
Asp718I	GGTACC	1 cut(s)	364
AspLEI	GCGC	3 cut(s)	1516, 2508, 2780
AspS9I	GGNCC	8 cut(s)	724, 1127, 1717, 2008, 2072, 2314, 2315, 2459
AsuC2I	CCSGG	2 cut(s)	2070, 2646
AsuHPI	GGTGA	5 cut(s)	1939, 2133, 2488, 2504, 2677
AvaI	CYCGRG	1 cut(s)	140
AvaII	GGWCC	3 cut(s)	724, 2008, 2459
AxyI	CCTNAGG	1 cut(s)	2338
BaeGI	GKGCMC	1 cut(s)	2318
BaeI	ACNNNNGTAYC	4 cut(s)	267, 300, 2700, 2733
BalI	TGGCCA	2 cut(s)	708, 1870
BanI	GGYRCC	1 cut(s)	364
BanII	GRGCYC	2 cut(s)	1059, 2318
BarI	GAAGNNNNNNTAC	2 cut(s)	2078, 2110
BauI	CACGAG	1 cut(s)	2439
Bbv12I	GWGCWC	1 cut(s)	223
BbvCI	CCTCAGC	1 cut(s)	815
BbvI	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2186, 2353, 2437
BccI	CCATC	5 cut(s)	584, 764, 1832, 1879, 2618
BceAI	ACGGC	4 cut(s)	70, 1673, 1704, 2468
BciT130I	CCWGG	4 cut(s)	528, 732, 1518, 2285
BclI	TGATCA	2 cut(s)	385, 2734
BcnI	CCSGG	2 cut(s)	2070, 2646
BcoDI	GTCTC	4 cut(s)	915, 1626, 2067, 2205
BcuI	ACTAGT	1 cut(s)	1505
BfaI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1506, 1580
BfmI	CTRYAG	3 cut(s)	76, 1048, 1821
BfuAI	ACCTGC	3 cut(s)	220, 259, 295
BglI	GCCNNNNNGGC	1 cut(s)	1135
BisI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2175, 2367, 2451
BlsI	GCNGC	9 cut(s)	7, 59, 697, 963, 1309, 1360, 2176, 2368, 2452
BmcAI	AGTACT	1 cut(s)	74
Bme1390I	CCNGG	6 cut(s)	528, 732, 1518, 2070, 2285, 2646
Bme18I	GGWCC	3 cut(s)	724, 2008, 2459
BmeRI	GACNNNNNGTC	1 cut(s)	2014
BmeT110I	CYCGRG	1 cut(s)	140
BmgT120I	GGNCC	8 cut(s)	724, 1127, 1717, 2008, 2072, 2314, 2315, 2459
BmiI	GGNNCC	8 cut(s)	366, 1113, 1270, 2009, 2010, 2316, 2317, 2460
BmrFI	CCNGG	6 cut(s)	528, 732, 1518, 2070, 2285, 2646
BmrI	ACTGGG	1 cut(s)	2480
BmsI	GCATC	9 cut(s)	307, 690, 1468, 1490, 1595, 1661, 2163, 2353, 2560
BmuI	ACTGGG	1 cut(s)	2480
Bpu10I	CCTNAGC	3 cut(s)	815, 1283, 2454
BpuEI	CTTGAG	4 cut(s)	62, 317, 1109, 1121
BpuMI	CCSGG	2 cut(s)	2070, 2646
BsaJI	CCNNGG	5 cut(s)	565, 730, 930, 1362, 1493
BsaXI	ACNNNNNCTCC	9 cut(s)	32, 1284, 1314, 2047, 2077, 2334, 2364, 2419, 2449
Bsc4I	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1523
Bse118I	RCCGGY	1 cut(s)	85
Bse1I	ACTGG	5 cut(s)	283, 368, 1317, 2475, 2596
Bse21I	CCTNAGG	1 cut(s)	2338
BseBI	CCWGG	4 cut(s)	528, 732, 1518, 2285
BseDI	CCNNGG	5 cut(s)	565, 730, 930, 1362, 1493
BseGI	GGATG	8 cut(s)	193, 1111, 1409, 1610, 1726, 2154, 2332, 2575
BseLI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1523
BseMII	CTCAG	6 cut(s)	600, 755, 806, 906, 1931, 2352
BseNI	ACTGG	5 cut(s)	283, 368, 1317, 2475, 2596
BseRI	GAGGAG	2 cut(s)	1749, 2055
BseSI	GKGCMC	1 cut(s)	2318
BseXI	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2186, 2353, 2437
BseYI	CCCAGC	1 cut(s)	2561
BsgI	GTGCAG	2 cut(s)	449, 1260
Bsh1285I	CGRYCG	1 cut(s)	952
BshFI	GGCC	8 cut(s)	708, 1129, 1138, 1719, 1870, 2073, 2316, 2483
BshNI	GGYRCC	1 cut(s)	364
BsiEI	CGRYCG	1 cut(s)	952
BsiHKAI	GWGCWC	1 cut(s)	223
BsiHKCI	CYCGRG	1 cut(s)	140
BsiSI	CCGG	4 cut(s)	50, 86, 2070, 2646
BslFI	GGGAC	2 cut(s)	634, 2021
BslI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1523
BsmAI	GTCTC	4 cut(s)	915, 1626, 2067, 2205
BsmFI	GGGAC	2 cut(s)	634, 2021
BsmI	GAATGC	3 cut(s)	500, 2095, 2508
BsnI	GGCC	8 cut(s)	708, 1129, 1138, 1719, 1870, 2073, 2316, 2483
BsoBI	CYCGRG	1 cut(s)	140
Bsp120I	GGGCCC	1 cut(s)	2314
Bsp1286I	GDGCHC	3 cut(s)	223, 1059, 2318
Bsp1407I	TGTACA	1 cut(s)	1723
Bsp19I	CCATGG	2 cut(s)	930, 1493
BspACI	CCGC	4 cut(s)	5, 8, 57, 2515
BspANI	GGCC	8 cut(s)	708, 1129, 1138, 1719, 1870, 2073, 2316, 2483
BspCNI	CTCAG	6 cut(s)	599, 756, 807, 907, 1930, 2351
BspLI	GGNNCC	8 cut(s)	366, 1113, 1270, 2009, 2010, 2316, 2317, 2460
BspMI	ACCTGC	3 cut(s)	220, 259, 295
BspPI	GGATC	5 cut(s)	92, 127, 499, 538, 689
BspT107I	GGYRCC	1 cut(s)	364
BsrFI	RCCGGY	1 cut(s)	85
BsrGI	TGTACA	1 cut(s)	1723
BsrI	ACTGG	5 cut(s)	283, 368, 1317, 2475, 2596
BssAI	RCCGGY	1 cut(s)	85
BssECI	CCNNGG	5 cut(s)	565, 730, 930, 1362, 1493
BssNAI	GTATAC	1 cut(s)	1075
BssSI	CACGAG	1 cut(s)	2439
BssT1I	CCWWGG	4 cut(s)	565, 930, 1362, 1493
Bst1107I	GTATAC	1 cut(s)	1075
Bst2BI	CACGAG	1 cut(s)	2439
Bst2UI	CCWGG	4 cut(s)	528, 732, 1518, 2285
Bst6I	CTCTTC	1 cut(s)	2203
BstAPI	GCANNNNNTGC	2 cut(s)	695, 1614
BstAUI	TGTACA	1 cut(s)	1723
BstDEI	CTNAG	8 cut(s)	586, 764, 815, 915, 1283, 1917, 2338, 2454
BstDSI	CCRYGG	2 cut(s)	930, 1493
BstENI	CCTNNNNNAGG	2 cut(s)	266, 531
BstF5I	GGATG	8 cut(s)	193, 1111, 1409, 1610, 1726, 2154, 2332, 2575
BstHHI	GCGC	3 cut(s)	1516, 2508, 2780
BstMAI	GTCTC	4 cut(s)	915, 1626, 2067, 2205
BstMCI	CGRYCG	1 cut(s)	952
BstNI	CCWGG	4 cut(s)	528, 732, 1518, 2285
BstNSI	RCATGY	6 cut(s)	313, 514, 910, 1184, 1188, 1617
BstSCI	CCNGG	6 cut(s)	526, 730, 1516, 2068, 2283, 2644
BstSFI	CTRYAG	3 cut(s)	76, 1048, 1821
BstSLI	GKGCMC	1 cut(s)	2318
BstV1I	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2186, 2353, 2437
BstX2I	RGATCY	1 cut(s)	132
BstXI	CCANNNNNNTGG	2 cut(s)	721, 1543
BstYI	RGATCY	1 cut(s)	132
BstZ17I	GTATAC	1 cut(s)	1075
Bsu36I	CCTNAGG	1 cut(s)	2338
BsuRI	GGCC	8 cut(s)	708, 1129, 1138, 1719, 1870, 2073, 2316, 2483
BtgI	CCRYGG	2 cut(s)	930, 1493
BtgZI	GCGATG	2 cut(s)	177, 713
BtsCI	GGATG	8 cut(s)	193, 1111, 1409, 1610, 1726, 2154, 2332, 2575
BtsI	GCAGTG	2 cut(s)	432, 1653
BtsIMutI	CAGTG	8 cut(s)	223, 276, 432, 996, 1057, 1653, 1812, 2625
BveI	ACCTGC	3 cut(s)	220, 259, 295
CaiI	CAGNNNCTG	4 cut(s)	695, 785, 1523, 1895
CfoI	GCGC	3 cut(s)	1516, 2508, 2780
Cfr10I	RCCGGY	1 cut(s)	85
Cfr13I	GGNCC	8 cut(s)	724, 1127, 1717, 2008, 2072, 2314, 2315, 2459
Csp6I	GTAC	9 cut(s)	73, 121, 284, 365, 1724, 1982, 2265, 2377, 2694
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 1600, 1635
CviQI	GTAC	9 cut(s)	73, 121, 284, 365, 1724, 1982, 2265, 2377, 2694
DdeI	CTNAG	8 cut(s)	586, 764, 815, 915, 1283, 1917, 2338, 2454
DraI	TTTAAA	1 cut(s)	2047
DriI	GACNNNNNGTC	1 cut(s)	2014
EaeI	YGGCCR	2 cut(s)	706, 1868
Eam1104I	CTCTTC	1 cut(s)	2203
Eam1105I	GACNNNNNGTC	1 cut(s)	2014
EarI	CTCTTC	1 cut(s)	2203
EciI	GGCGGA	1 cut(s)	23
Eco130I	CCWWGG	4 cut(s)	565, 930, 1362, 1493
Eco24I	GRGCYC	2 cut(s)	1059, 2318
Eco32I	GATATC	1 cut(s)	2656
Eco47I	GGWCC	3 cut(s)	724, 2008, 2459
Eco57I	CTGAAG	2 cut(s)	251, 1668
Eco81I	CCTNAGG	1 cut(s)	2338
Eco88I	CYCGRG	1 cut(s)	140
EcoNI	CCTNNNNNAGG	2 cut(s)	266, 531
EcoO109I	RGGNCCY	2 cut(s)	2008, 2315
EcoRII	CCWGG	4 cut(s)	526, 730, 1516, 2283
EcoRV	GATATC	1 cut(s)	2656
EcoT14I	CCWWGG	4 cut(s)	565, 930, 1362, 1493
EcoT22I	ATGCAT	5 cut(s)	502, 1186, 1483, 1619, 2005
EcoT38I	GRGCYC	2 cut(s)	1059, 2318
ErhI	CCWWGG	4 cut(s)	565, 930, 1362, 1493
FalI	AAGNNNNNCTT	2 cut(s)	2251, 2283
FaqI	GGGAC	2 cut(s)	634, 2021
FauNDI	CATATG	1 cut(s)	1936
FbaI	TGATCA	2 cut(s)	385, 2734
FblI	GTMKAC	2 cut(s)	1074, 1408
Fnu4HI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2175, 2367, 2451
FokI	GGATG	8 cut(s)	200, 1118, 1416, 1617, 1713, 2141, 2319, 2582
FriOI	GRGCYC	2 cut(s)	1059, 2318
Fsp4HI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2175, 2367, 2451
FspBI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1506, 1580
GlaI	GCGC	3 cut(s)	1515, 2507, 2779
GluI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2175, 2367, 2451
GsaI	CCCAGC	1 cut(s)	2565
HaeIII	GGCC	8 cut(s)	708, 1129, 1138, 1719, 1870, 2073, 2316, 2483
HapII	CCGG	4 cut(s)	50, 86, 2070, 2646
HhaI	GCGC	3 cut(s)	1516, 2508, 2780
Hin6I	GCGC	3 cut(s)	1514, 2506, 2778
HinP1I	GCGC	3 cut(s)	1514, 2506, 2778
HindIII	AAGCTT	1 cut(s)	1377
HpaII	CCGG	4 cut(s)	50, 86, 2070, 2646
HphI	GGTGA	5 cut(s)	1939, 2133, 2488, 2504, 2677
Hpy166II	GTNNAC	6 cut(s)	284, 1045, 1075, 1409, 1724, 2019
Hpy188I	TCNGA	9 cut(s)	68, 418, 589, 798, 1158, 1572, 1920, 2660, 2772
Hpy8I	GTNNAC	6 cut(s)	284, 1045, 1075, 1409, 1724, 2019
Hpy99I	CGWCG	1 cut(s)	2238
HpyAV	CCTTC	7 cut(s)	146, 272, 854, 1995, 2381, 2473, 2627
HpyCH4IV	ACGT	1 cut(s)	2194
HpyF3I	CTNAG	8 cut(s)	586, 764, 815, 915, 1283, 1917, 2338, 2454
HpySE526I	ACGT	1 cut(s)	2194
HspAI	GCGC	3 cut(s)	1514, 2506, 2778
KflI	GGGWCCC	1 cut(s)	2008
KpnI	GGTACC	1 cut(s)	368
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	2 cut(s)	385, 2734
LmnI	GCTCC	4 cut(s)	226, 1111, 1355, 2719
Lsp1109I	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2186, 2353, 2437
LweI	GCATC	9 cut(s)	307, 690, 1468, 1490, 1595, 1661, 2163, 2353, 2560
MaeI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1506, 1580
MaeII	ACGT	1 cut(s)	2194
MaeIII	GTNAC	6 cut(s)	155, 775, 802, 955, 1531, 2665
MboII	GAAGA	4 cut(s)	257, 1858, 2098, 2220
MflI	RGATCY	1 cut(s)	132
MhlI	GDGCHC	3 cut(s)	223, 1059, 2318
MlsI	TGGCCA	2 cut(s)	708, 1870
MluNI	TGGCCA	2 cut(s)	708, 1870
MlyI	GAGTC	3 cut(s)	114, 152, 267
MmeI	TCCRAC	2 cut(s)	1183, 1381
Mox20I	TGGCCA	2 cut(s)	708, 1870
Mph1103I	ATGCAT	5 cut(s)	502, 1186, 1483, 1619, 2005
MroNI	GCCGGC	1 cut(s)	85
MroXI	GAANNNNTTC	1 cut(s)	17
MscI	TGGCCA	2 cut(s)	708, 1870
MseI	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1500, 1587, 1733, 2046
MslI	CAYNNNNRTG	5 cut(s)	638, 719, 1146, 1293, 2689
Msp20I	TGGCCA	2 cut(s)	708, 1870
MspA1I	CMGCKG	1 cut(s)	2147
MspI	CCGG	4 cut(s)	50, 86, 2070, 2646
MspR9I	CCNGG	6 cut(s)	528, 732, 1518, 2070, 2285, 2646
Mva1269I	GAATGC	3 cut(s)	500, 2095, 2508
MvaI	CCWGG	4 cut(s)	528, 732, 1518, 2285
NaeI	GCCGGC	1 cut(s)	87
NciI	CCSGG	2 cut(s)	2070, 2646
NcoI	CCATGG	2 cut(s)	930, 1493
NdeI	CATATG	1 cut(s)	1936
NgoMIV	GCCGGC	1 cut(s)	85
NlaIV	GGNNCC	8 cut(s)	366, 1113, 1270, 2009, 2010, 2316, 2317, 2460
NmuCI	GTSAC	3 cut(s)	775, 955, 2665
NsiI	ATGCAT	5 cut(s)	502, 1186, 1483, 1619, 2005
NspI	RCATGY	6 cut(s)	313, 514, 910, 1184, 1188, 1617
PaeI	GCATGC	3 cut(s)	313, 1184, 1188
PaeR7I	CTCGAG	1 cut(s)	140
PciI	ACATGT	1 cut(s)	906
PctI	GAATGC	3 cut(s)	500, 2095, 2508
PdiI	GCCGGC	1 cut(s)	87
PdmI	GAANNNNTTC	1 cut(s)	17
PfeI	GAWTC	9 cut(s)	13, 238, 341, 626, 1013, 1232, 1573, 1969, 2767
PflFI	GACNNNGTC	2 cut(s)	2059, 2646
PflMI	CCANNNNNTGG	1 cut(s)	1523
PfoI	TCCNGGA	1 cut(s)	526
PkrI	GCNGC	9 cut(s)	7, 59, 697, 963, 1309, 1360, 2176, 2368, 2452
Ple19I	CGATCG	1 cut(s)	952
PleI	GAGTC	3 cut(s)	113, 151, 266
PpsI	GAGTC	3 cut(s)	113, 151, 266
PpuMI	RGGWCCY	1 cut(s)	2008
PscI	ACATGT	1 cut(s)	906
Psp5II	RGGWCCY	1 cut(s)	2008
Psp6I	CCWGG	4 cut(s)	526, 730, 1516, 2283
PspFI	CCCAGC	1 cut(s)	2561
PspGI	CCWGG	4 cut(s)	526, 730, 1516, 2283
PspN4I	GGNNCC	8 cut(s)	366, 1113, 1270, 2009, 2010, 2316, 2317, 2460
PspOMI	GGGCCC	1 cut(s)	2314
PspPI	GGNCC	8 cut(s)	724, 1127, 1717, 2008, 2072, 2314, 2315, 2459
PspPPI	RGGWCCY	1 cut(s)	2008
PstNI	CAGNNNCTG	4 cut(s)	695, 785, 1523, 1895
PsuI	RGATCY	1 cut(s)	132
PsyI	GACNNNGTC	2 cut(s)	2059, 2646
PvuI	CGATCG	1 cut(s)	952
PvuII	CAGCTG	1 cut(s)	2147
RsaI	GTAC	9 cut(s)	74, 122, 285, 366, 1725, 1983, 2266, 2378, 2695
RsaNI	GTAC	9 cut(s)	73, 121, 284, 365, 1724, 1982, 2265, 2377, 2694
RseI	CAYNNNNRTG	5 cut(s)	638, 719, 1146, 1293, 2689
SaqAI	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1500, 1587, 1733, 2046
SatI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2175, 2367, 2451
Sau96I	GGNCC	8 cut(s)	724, 1127, 1717, 2008, 2072, 2314, 2315, 2459
ScaI	AGTACT	1 cut(s)	74
SchI	GAGTC	3 cut(s)	114, 152, 267
ScrFI	CCNGG	6 cut(s)	528, 732, 1518, 2070, 2285, 2646
SduI	GDGCHC	3 cut(s)	223, 1059, 2318
SfaNI	GCATC	9 cut(s)	307, 690, 1468, 1490, 1595, 1661, 2163, 2353, 2560
SfcI	CTRYAG	3 cut(s)	76, 1048, 1821
SfiI	GGCCNNNNNGGCC	1 cut(s)	1135
Sfr274I	CTCGAG	1 cut(s)	140
SinI	GGWCC	3 cut(s)	724, 2008, 2459
SlaI	CTCGAG	1 cut(s)	140
SmiMI	CAYNNNNRTG	5 cut(s)	638, 719, 1146, 1293, 2689
SmlI	CTYRAG	5 cut(s)	41, 140, 332, 1088, 1100
SmoI	CTYRAG	5 cut(s)	41, 140, 332, 1088, 1100
SpeI	ACTAGT	1 cut(s)	1505
SphI	GCATGC	3 cut(s)	313, 1184, 1188
SsiI	CCGC	4 cut(s)	5, 8, 57, 2515
SspI	AATATT	1 cut(s)	2278
SspMI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1506, 1580
StyD4I	CCNGG	6 cut(s)	526, 730, 1516, 2068, 2283, 2644
StyI	CCWWGG	4 cut(s)	565, 930, 1362, 1493
TaiI	ACGT	1 cut(s)	2197
TaqI	TCGA	5 cut(s)	108, 141, 684, 948, 2613
TatI	WGTACW	3 cut(s)	72, 1723, 2376
TauI	GCSGC	2 cut(s)	8, 60
TfiI	GAWTC	9 cut(s)	13, 238, 341, 626, 1013, 1232, 1573, 1969, 2767
Tru1I	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1500, 1587, 1733, 2046
Tru9I	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1500, 1587, 1733, 2046
TscAI	CASTG	8 cut(s)	223, 283, 432, 1003, 1057, 1653, 1819, 2632
TseFI	GTSAC	3 cut(s)	775, 955, 2665
TseI	GCWGC	7 cut(s)	695, 961, 1307, 1358, 2174, 2366, 2450
Tsp45I	GTSAC	3 cut(s)	775, 955, 2665
TspDTI	ATGAA	9 cut(s)	338, 480, 533, 1181, 1253, 1656, 1953, 2318, 2387
TspGWI	ACGGA	7 cut(s)	6, 143, 231, 663, 1679, 2344, 2754
TspRI	CASTG	8 cut(s)	223, 283, 432, 1003, 1057, 1653, 1819, 2632
Tth111I	GACNNNGTC	2 cut(s)	2059, 2646
Van91I	CCANNNNNTGG	1 cut(s)	1523
VpaK11BI	GGWCC	3 cut(s)	724, 2008, 2459
XagI	CCTNNNNNAGG	2 cut(s)	266, 531
XapI	RAATTY	3 cut(s)	443, 1624, 1708
XceI	RCATGY	6 cut(s)	313, 514, 910, 1184, 1188, 1617
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2185
XhoI	CTCGAG	1 cut(s)	140
XmiI	GTMKAC	2 cut(s)	1074, 1408
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1506, 1580
ZrmI	AGTACT	1 cut(s)	74
Zsp2I	ATGCAT	5 cut(s)	502, 1186, 1483, 1619, 2005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.