Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Reverse (-)

19549683 .. 19555524

Gene Structure 5842 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1237700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGGCGGCGGCGAATCCGTTTCAGAAGATACTGACGGCGCTCGAGAAGCCGGACGGCGGCGAATTCGGCAAATACTACAGCTTGCCGGCTCTGAACGATCCCAGAATCGAGAAGCTGCCATTCTCTATTAAGATCCTTCTCGAATCCGCAATTCGTAACTGTGATGAGTTTCAAGTGAAGAGTAATGATGTTGAGAAAATTATTGATTGGGAGAACACTTCTCCCAAGCAGGTTGAGATCCCATTCAAACCTGCTAGAGTCCTGCTTCAGGACTTTACTGGAGTACCAGCTGTCGTTGATCTAGCTTGTATGCGTGATGCCATGAACAATCTGAAGGGGGATTCTAACAAGATTAACCCGTTGGTCCCAGTAGATCTTGTCATTGATCACTCGGTTCAAGTAGATATTGCGAGATCAGAAAATGCAGTGCAGGCAAATATGGAACTTGAGTTCCAGCGAAACAAAGAACGGTTTGGTTTTCTGAAATGGGGATCAAATGCTTTCCATAACATGCTCGTTGTTCCTCCTGGATCAGGGATTGTTCATCAGGTTAATTTGGAATACCTTGGTCGAGTTGTGTTCAACACAGACGGCTTGCTTTATCCTGATAGTGTTGTTGGGACAGATTCACACACAACTATGATAGACGGATTAGGTGTCGCTGGCTGGGGTGTGGGTGGTATCGAAGCAGAAGCCACTATGCTTGGCCAGCCCATGAGCATGGTCCTGCCTGGTGTTGTTGGGTTTAAATTATTAGGAAAACTGAGAGACGGTGTGACAGCTACTGACTTGGTTCTGACAGTAACTCAAATGCTGAGGAAGCATGGAGTTGTTGGCAAGTTTGTGGAGTTCTATGGGGAAGGCATGAGTGAATTGTCACTAGCAGACCGTGCCACTATTGCTAACATGTCTCCTGAGTATGGTGCAACCATGGGCTTCTTCCCTGTGGATCATGTCACCCTGCAATATCTAAAACTTACTGGCAGAAGTGATGACAAGGTCGCTCTGATAGAATCCTATTTACGGGCTAATAAGATGTTTGTTGACTACAATGAGATCGAGAGAGTGTACTCCTCCTATCTTGAGCTAAATCTTAATGAAGTGGAACCATGCATATCAGGTCCAAAGAGGCCACATGATCGTGTTGCTTTGAAAGAAATGAAAGTGGATTGGCATGCATGCCTTGACAATAGAGTTGGATTTAAGGGTTTTTCTGTGCCAAAGGAATCTCAAAGTAAAGTTGTAGAATTCAATTTTCAAGGATCCCCAGCACAGCTTAGGCATGGGGATGTTGTTATTGCAGCTATCACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTTGGAGCTGCTTTGGTTGCAAAGAAAGCTTGTGATTTAGGATTGGAGGTCAAGCCATGGATTAAGACAAGTCTTGCTCCCGGTTCTGGGGTTGTAACCAAATACTTGCAAAAGAGTGGGTTGCAGAAGTATTTGAATCAGTTAGGGTTTCATATTGTTGGGTATGGATGCACCACTTGCATTGGGAATTCAGGAGATATTGATGATGCAGTAGCGGCTGCAATTACAGAAAATGATGTTGTAGCTGCGGCTGTATTATCTGGAAATAGGAATTTTGAGGGTCGTGTGCATCCGTTAACAAGGGCTAATTATCTTGCTTCTCCTCCCCTTGTTGTTGCCTATGCACTTGCTGGAACGGTAGATATTGATTTCGAAACAGAACCAATTGGGGTGGGAAAAGATGGGAAAAAGATATTTTTCAGGGACATCTGGCCATCCAATGAAGAAGTAGCAACGGTTGTGCAATCAAATGTGCTTCCTCATATGTTTATGGCCACCTATGAAGCAATCACCAAAGGAAATCCAATGTGGAATCAGTTATCTGTACCATCTGGCACTCTTTATGCCTGGGACCCAAAATCAACTTATATACATGAACCGCCGTATTTTAAGGATATGACCATGTCTCCCCCGGGCGCTCATGGAGTGAAGAATGCTTACTGTTTGCTCAACTTTGGAGATAGTATTACAACCGATCACATCTCACCAGCTGGTAGCATTCACAAAGACAGCCCAGCAGCCAAATACCTACTGGAACGTGGCGTTGATAGAAGAGACTTTAACTCCTATGGAAGTCGCCGTGGTAATGATGAGGTTATGGCAAGAGGCACTTTTGCAAATATTCGGCTGGTCAATAAGTTTTTGAAAGGAGAAGTTGGTCCCAAAACAATCCACATTCCCACTGGAGAGAAACTGTCCGTGTTTGATGCTGCTATGAGGTACAAAAGCGAAGGACATGATACAATTATTTTGGCTGGTGCCGAGTATGGAAGTGGAAGTTCTCGTGATTGGGCTGCAAAGGGTCCCATGCTACTGGGTGTGAAGGCGGTCATAGCAAAGAGCTTTGAGCGCATTCACCGTAGTAATCTTGTTGGCATGGGCATCATTCCACTATGTTTCAAGACTGGGGAGGATGCCGACTCTCTTGGTTTAACTGGGGAGGAACGCTACACCATCGATCTTCCAAGCAGTGTTAGTGAAATTAGACCCGGTCAGGATATCACAGTGGTGACAGATAACGGCAAGTCCTTCATATGTACGCTACGTTTCGATACAGAGGTCGAGCTGGCCTATTTCGACCACGGGGGCATTTTGCAATACGTCATCAGGAATCTGATCAAAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

897

Amino Acids

98.0

Weight (kDa)

5.86

Isoelectric Point (pI)

36.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	65 - 566	7.4e-169	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	695 - 822	2.3e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	220, 259
AccB1I	GGYRCC	1 cut(s)	2321
AciI	CCGC	8 cut(s)	5, 8, 57, 147, 1559, 1592, 1943, 2390
AclWI	GGATC	8 cut(s)	92, 127, 232, 499, 538, 957, 1257, 1270
AcoI	YGGCCR	3 cut(s)	706, 1775, 1836
AcsI	RAATTY	4 cut(s)	62, 1247, 1531, 1615
AcuI	CTGAAG	2 cut(s)	251, 353
AfaI	GTAC	5 cut(s)	285, 1070, 1890, 2285, 2602
AfiI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 920, 1023, 1430, 1431
AflIII	ACRYGT	1 cut(s)	906
AjnI	CCWGG	3 cut(s)	526, 730, 1910
AleI	CACNNNNGTG	1 cut(s)	2570
AloI	GAACNNNNNNTCC	4 cut(s)	434, 435, 466, 467
Alw26I	GTCTC	4 cut(s)	762, 915, 1974, 2112
AlwI	GGATC	8 cut(s)	92, 127, 232, 499, 538, 957, 1257, 1270
AlwNI	CAGNNNCTG	1 cut(s)	785
Ama87I	CYCGRG	2 cut(s)	41, 1975
AoxI	GGCC	5 cut(s)	706, 1130, 1775, 1836, 2631
ApeKI	GCWGC	8 cut(s)	115, 1301, 1352, 1562, 1589, 2081, 2273, 2357
ApoI	RAATTY	4 cut(s)	62, 1247, 1531, 1615
AspLEI	GCGC	3 cut(s)	40, 1982, 2415
AspS9I	GGNCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
AsuC2I	CCSGG	4 cut(s)	1425, 1976, 1977, 2553
AsuHPI	GGTGA	6 cut(s)	949, 1300, 1846, 2040, 2411, 2584
AsuII	TTCGAA	1 cut(s)	1716
AvaI	CYCGRG	2 cut(s)	41, 1975
AvaII	GGWCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
BaeI	ACNNNNGTAYC	4 cut(s)	267, 300, 2607, 2640
BalI	TGGCCA	3 cut(s)	708, 1777, 1838
BamHI	GGATCC	1 cut(s)	1262
BanI	GGYRCC	1 cut(s)	2321
BarI	GAAGNNNNNNTAC	2 cut(s)	1985, 2017
BauI	CACGAG	1 cut(s)	2346
BbvCI	CCTCAGC	1 cut(s)	815
BbvI	GCAGC	8 cut(s)	102, 1313, 1339, 1549, 1576, 2093, 2260, 2344
BccI	CCATC	4 cut(s)	1739, 1786, 1900, 2525
BceAI	ACGGC	6 cut(s)	51, 70, 607, 1930, 2127, 2599
BciT130I	CCWGG	3 cut(s)	528, 732, 1912
BclI	TGATCA	2 cut(s)	385, 2679
BcnI	CCSGG	4 cut(s)	1425, 1976, 1977, 2553
BcoDI	GTCTC	4 cut(s)	762, 915, 1974, 2112
BfaI	CTAG	4 cut(s)	255, 302, 881, 1335
BfmI	CTRYAG	1 cut(s)	76
BfoI	RGCGCY	2 cut(s)	41, 1983
BfuAI	ACCTGC	2 cut(s)	220, 259
BglII	AGATCT	1 cut(s)	373
Bme1390I	CCNGG	7 cut(s)	528, 732, 1425, 1912, 1976, 1977, 2553
Bme18I	GGWCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
BmeT110I	CYCGRG	2 cut(s)	41, 1975
BmgT120I	GGNCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
BmiI	GGNNCC	9 cut(s)	366, 1107, 1264, 1916, 1917, 2224, 2323, 2367, 2368
BmrFI	CCNGG	7 cut(s)	528, 732, 1425, 1912, 1976, 1977, 2553
BmrI	ACTGGG	4 cut(s)	362, 2387, 2478, 2508
BmsI	GCATC	7 cut(s)	307, 1502, 1540, 1642, 2260, 2454, 2467
BmuI	ACTGGG	4 cut(s)	362, 2387, 2478, 2508
BpmI	CTGGAG	2 cut(s)	300, 2268
Bpu10I	CCTNAGC	2 cut(s)	815, 1277
Bpu14I	TTCGAA	1 cut(s)	1716
BpuEI	CTTGAG	2 cut(s)	467, 1103
BpuMI	CCSGG	4 cut(s)	1425, 1976, 1977, 2553
Bsa29I	ATCGAT	1 cut(s)	2520
BsaJI	CCNNGG	8 cut(s)	565, 930, 1400, 1911, 1974, 1975, 2143, 2644
BsaXI	ACNNNNNCTCC	4 cut(s)	1954, 1984, 2111, 2141
Bsc4I	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 920, 1023, 1430, 1431
Bse118I	RCCGGY	1 cut(s)	85
Bse1I	ACTGG	9 cut(s)	283, 368, 985, 1311, 2100, 2251, 2382, 2473, 2503
BseBI	CCWGG	3 cut(s)	528, 732, 1912
BseCI	ATCGAT	1 cut(s)	2520
BseDI	CCNNGG	8 cut(s)	565, 930, 1400, 1911, 1974, 1975, 2143, 2644
BseGI	GGATG	5 cut(s)	1294, 1517, 1633, 1778, 2482
BseLI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 920, 1023, 1430, 1431
BseMII	CTCAG	3 cut(s)	755, 806, 906
BseNI	ACTGG	9 cut(s)	283, 368, 985, 1311, 2100, 2251, 2382, 2473, 2503
BseRI	GAGGAG	2 cut(s)	1063, 1656
BseXI	GCAGC	8 cut(s)	102, 1313, 1339, 1549, 1576, 2093, 2260, 2344
BseYI	CCCAGC	3 cut(s)	666, 1267, 2077
BsgI	GTGCAG	1 cut(s)	449
BshFI	GGCC	5 cut(s)	708, 1132, 1777, 1838, 2633
BshNI	GGYRCC	1 cut(s)	2321
BshVI	ATCGAT	1 cut(s)	2520
BsiHKCI	CYCGRG	2 cut(s)	41, 1975
BsiSI	CCGG	5 cut(s)	50, 86, 1425, 1976, 2553
BslFI	GGGAC	6 cut(s)	350, 634, 1781, 1928, 2208, 2352
BslI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 920, 1023, 1430, 1431
BsmAI	GTCTC	4 cut(s)	762, 915, 1974, 2112
BsmBI	CGTCTC	1 cut(s)	762
BsmFI	GGGAC	6 cut(s)	350, 634, 1781, 1928, 2208, 2352
BsmI	GAATGC	3 cut(s)	2002, 2061, 2415
BsnI	GGCC	5 cut(s)	708, 1132, 1777, 1838, 2633
BsoBI	CYCGRG	2 cut(s)	41, 1975
Bsp119I	TTCGAA	1 cut(s)	1716
Bsp19I	CCATGG	2 cut(s)	930, 1400
BspACI	CCGC	8 cut(s)	5, 8, 57, 147, 1559, 1592, 1943, 2390
BspANI	GGCC	5 cut(s)	708, 1132, 1777, 1838, 2633
BspCNI	CTCAG	3 cut(s)	756, 807, 907
BspDI	ATCGAT	1 cut(s)	2520
BspLI	GGNNCC	9 cut(s)	366, 1107, 1264, 1916, 1917, 2224, 2323, 2367, 2368
BspMI	ACCTGC	2 cut(s)	220, 259
BspPI	GGATC	8 cut(s)	92, 127, 232, 499, 538, 957, 1257, 1270
BspT104I	TTCGAA	1 cut(s)	1716
BspT107I	GGYRCC	1 cut(s)	2321
BsrFI	RCCGGY	1 cut(s)	85
BsrI	ACTGG	9 cut(s)	283, 368, 985, 1311, 2100, 2251, 2382, 2473, 2503
BssAI	RCCGGY	1 cut(s)	85
BssECI	CCNNGG	8 cut(s)	565, 930, 1400, 1911, 1974, 1975, 2143, 2644
BssSI	CACGAG	1 cut(s)	2346
BssT1I	CCWWGG	3 cut(s)	565, 930, 1400
Bst2BI	CACGAG	1 cut(s)	2346
Bst2UI	CCWGG	3 cut(s)	528, 732, 1912
Bst6I	CTCTTC	2 cut(s)	173, 2110
BstAPI	GCANNNNNTGC	2 cut(s)	890, 1521
BstBI	TTCGAA	1 cut(s)	1716
BstC8I	GCNNGC	9 cut(s)	83, 87, 432, 596, 664, 710, 1176, 1180, 2631
BstDEI	CTNAG	4 cut(s)	764, 815, 915, 1277
BstDSI	CCRYGG	4 cut(s)	930, 1400, 2143, 2644
BstENI	CCTNNNNNAGG	2 cut(s)	266, 531
BstF5I	GGATG	5 cut(s)	1294, 1517, 1633, 1778, 2482
BstH2I	RGCGCY	2 cut(s)	41, 1983
BstHHI	GCGC	3 cut(s)	40, 1982, 2415
BstMAI	GTCTC	4 cut(s)	762, 915, 1974, 2112
BstNI	CCWGG	3 cut(s)	528, 732, 1912
BstNSI	RCATGY	4 cut(s)	514, 910, 1178, 1182
BstSCI	CCNGG	7 cut(s)	526, 730, 1423, 1910, 1974, 1975, 2551
BstSFI	CTRYAG	1 cut(s)	76
BstV1I	GCAGC	8 cut(s)	102, 1313, 1339, 1549, 1576, 2093, 2260, 2344
BstX2I	RGATCY	4 cut(s)	132, 237, 373, 1262
BstXI	CCANNNNNNTGG	2 cut(s)	721, 2377
BstYI	RGATCY	4 cut(s)	132, 237, 373, 1262
Bsu15I	ATCGAT	1 cut(s)	2520
BsuRI	GGCC	5 cut(s)	708, 1132, 1777, 1838, 2633
BsuTUI	ATCGAT	1 cut(s)	2520
BtgI	CCRYGG	4 cut(s)	930, 1400, 2143, 2644
BtsCI	GGATG	5 cut(s)	1294, 1517, 1633, 1778, 2482
BtsI	GCAGTG	2 cut(s)	432, 2539
BtsIMutI	CAGTG	4 cut(s)	432, 2244, 2539, 2574
BveI	ACCTGC	2 cut(s)	220, 259
Cac8I	GCNNGC	9 cut(s)	83, 87, 432, 596, 664, 710, 1176, 1180, 2631
CaiI	CAGNNNCTG	1 cut(s)	785
CfoI	GCGC	3 cut(s)	40, 1982, 2415
Cfr10I	RCCGGY	1 cut(s)	85
Cfr13I	GGNCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
Cfr9I	CCCGGG	1 cut(s)	1975
ClaI	ATCGAT	1 cut(s)	2520
Csp6I	GTAC	5 cut(s)	284, 1069, 1889, 2284, 2601
CspCI	CAANNNNNGTGG	6 cut(s)	685, 720, 1507, 1542, 1716, 1751
CviQI	GTAC	5 cut(s)	284, 1069, 1889, 2284, 2601
DdeI	CTNAG	4 cut(s)	764, 815, 915, 1277
DraI	TTTAAA	1 cut(s)	748
EaeI	YGGCCR	3 cut(s)	706, 1775, 1836
Eam1104I	CTCTTC	2 cut(s)	173, 2110
EarI	CTCTTC	2 cut(s)	173, 2110
Eco130I	CCWWGG	3 cut(s)	565, 930, 1400
Eco32I	GATATC	1 cut(s)	2563
Eco47I	GGWCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
Eco57I	CTGAAG	2 cut(s)	251, 353
Eco88I	CYCGRG	2 cut(s)	41, 1975
EcoNI	CCTNNNNNAGG	2 cut(s)	266, 531
EcoO109I	RGGNCCY	2 cut(s)	1915, 2366
EcoRI	GAATTC	3 cut(s)	62, 1247, 1531
EcoRII	CCWGG	3 cut(s)	526, 730, 1910
EcoRV	GATATC	1 cut(s)	2563
EcoT14I	CCWWGG	3 cut(s)	565, 930, 1400
EcoT22I	ATGCAT	2 cut(s)	1115, 1180
ErhI	CCWWGG	3 cut(s)	565, 930, 1400
Esp3I	CGTCTC	1 cut(s)	762
FalI	AAGNNNNNCTT	2 cut(s)	2158, 2190
FaqI	GGGAC	6 cut(s)	350, 634, 1781, 1928, 2208, 2352
FauNDI	CATATG	2 cut(s)	1827, 2597
FbaI	TGATCA	2 cut(s)	385, 2679
FokI	GGATG	5 cut(s)	1301, 1524, 1620, 1765, 2489
FspBI	CTAG	4 cut(s)	255, 302, 881, 1335
GlaI	GCGC	3 cut(s)	39, 1981, 2414
GsaI	CCCAGC	3 cut(s)	670, 1271, 2081
GsuI	CTGGAG	2 cut(s)	300, 2268
HaeII	RGCGCY	2 cut(s)	41, 1983
HaeIII	GGCC	5 cut(s)	708, 1132, 1777, 1838, 2633
HapII	CCGG	5 cut(s)	50, 86, 1425, 1976, 2553
HhaI	GCGC	3 cut(s)	40, 1982, 2415
Hin6I	GCGC	3 cut(s)	38, 1980, 2413
HinP1I	GCGC	3 cut(s)	38, 1980, 2413
HincII	GTYRAC	2 cut(s)	1045, 1641
HindII	GTYRAC	2 cut(s)	1045, 1641
HindIII	AAGCTT	1 cut(s)	1371
HpaI	GTTAAC	1 cut(s)	1641
HpaII	CCGG	5 cut(s)	50, 86, 1425, 1976, 2553
HphI	GGTGA	6 cut(s)	949, 1300, 1846, 2040, 2411, 2584
Hpy166II	GTNNAC	3 cut(s)	1045, 1069, 1641
Hpy188I	TCNGA	8 cut(s)	24, 93, 333, 418, 483, 798, 1008, 2679
Hpy8I	GTNNAC	3 cut(s)	1045, 1069, 1641
HpyAV	CCTTC	6 cut(s)	146, 328, 854, 2288, 2380, 2602
HpyCH4IV	ACGT	3 cut(s)	2101, 2608, 2664
HpyF3I	CTNAG	4 cut(s)	764, 815, 915, 1277
HpySE526I	ACGT	3 cut(s)	2101, 2608, 2664
HspAI	GCGC	3 cut(s)	38, 1980, 2413
KflI	GGGWCCC	2 cut(s)	1915, 2366
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	2 cut(s)	385, 2679
KspAI	GTTAAC	1 cut(s)	1641
LmnI	GCTCC	2 cut(s)	1349, 1426
Lsp1109I	GCAGC	8 cut(s)	102, 1313, 1339, 1549, 1576, 2093, 2260, 2344
LweI	GCATC	7 cut(s)	307, 1502, 1540, 1642, 2260, 2454, 2467
MaeI	CTAG	4 cut(s)	255, 302, 881, 1335
MaeII	ACGT	3 cut(s)	2101, 2608, 2664
MaeIII	GTNAC	7 cut(s)	155, 775, 802, 876, 955, 1438, 2572
MboII	GAAGA	7 cut(s)	37, 190, 931, 1799, 2005, 2127, 2516
MfeI	CAATTG	1 cut(s)	1728
MflI	RGATCY	4 cut(s)	132, 237, 373, 1262
MlsI	TGGCCA	3 cut(s)	708, 1777, 1838
MluNI	TGGCCA	3 cut(s)	708, 1777, 1838
MlyI	GAGTC	2 cut(s)	267, 2477
MmeI	TCCRAC	1 cut(s)	1177
Mox20I	TGGCCA	3 cut(s)	708, 1777, 1838
Mph1103I	ATGCAT	2 cut(s)	1115, 1180
MroNI	GCCGGC	1 cut(s)	85
MscI	TGGCCA	3 cut(s)	708, 1777, 1838
MslI	CAYNNNNRTG	5 cut(s)	638, 719, 1140, 1287, 2570
Msp20I	TGGCCA	3 cut(s)	708, 1777, 1838
MspA1I	CMGCKG	2 cut(s)	290, 2054
MspI	CCGG	5 cut(s)	50, 86, 1425, 1976, 2553
MspR9I	CCNGG	7 cut(s)	528, 732, 1425, 1912, 1976, 1977, 2553
MunI	CAATTG	1 cut(s)	1728
Mva1269I	GAATGC	3 cut(s)	2002, 2061, 2415
MvaI	CCWGG	3 cut(s)	528, 732, 1912
NaeI	GCCGGC	1 cut(s)	87
NciI	CCSGG	4 cut(s)	1425, 1976, 1977, 2553
NcoI	CCATGG	2 cut(s)	930, 1400
NdeI	CATATG	2 cut(s)	1827, 2597
NgoMIV	GCCGGC	1 cut(s)	85
NlaIV	GGNNCC	9 cut(s)	366, 1107, 1264, 1916, 1917, 2224, 2323, 2367, 2368
NmeAIII	GCCGAG	1 cut(s)	2350
NmuCI	GTSAC	4 cut(s)	775, 876, 955, 2572
NsiI	ATGCAT	2 cut(s)	1115, 1180
NspI	RCATGY	4 cut(s)	514, 910, 1178, 1182
NspV	TTCGAA	1 cut(s)	1716
OliI	CACNNNNGTG	1 cut(s)	2570
PaeI	GCATGC	2 cut(s)	1178, 1182
PaeR7I	CTCGAG	1 cut(s)	41
PciI	ACATGT	1 cut(s)	906
PctI	GAATGC	3 cut(s)	2002, 2061, 2415
PdiI	GCCGGC	1 cut(s)	87
PflFI	GACNNNGTC	3 cut(s)	998, 1966, 2553
PfoI	TCCNGGA	1 cut(s)	526
PleI	GAGTC	2 cut(s)	266, 2477
PpsI	GAGTC	2 cut(s)	266, 2477
PpuMI	RGGWCCY	2 cut(s)	1915, 2366
PscI	ACATGT	1 cut(s)	906
Psp5II	RGGWCCY	2 cut(s)	1915, 2366
Psp6I	CCWGG	3 cut(s)	526, 730, 1910
PspFI	CCCAGC	3 cut(s)	666, 1267, 2077
PspGI	CCWGG	3 cut(s)	526, 730, 1910
PspN4I	GGNNCC	9 cut(s)	366, 1107, 1264, 1916, 1917, 2224, 2323, 2367, 2368
PspPI	GGNCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
PspPPI	RGGWCCY	2 cut(s)	1915, 2366
PstNI	CAGNNNCTG	1 cut(s)	785
PsuI	RGATCY	4 cut(s)	132, 237, 373, 1262
PsyI	GACNNNGTC	3 cut(s)	998, 1966, 2553
PvuII	CAGCTG	2 cut(s)	290, 2054
RsaI	GTAC	5 cut(s)	285, 1070, 1890, 2285, 2602
RsaNI	GTAC	5 cut(s)	284, 1069, 1889, 2284, 2601
RseI	CAYNNNNRTG	5 cut(s)	638, 719, 1140, 1287, 2570
Sau96I	GGNCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
SchI	GAGTC	2 cut(s)	267, 2477
ScrFI	CCNGG	7 cut(s)	528, 732, 1425, 1912, 1976, 1977, 2553
SfaNI	GCATC	7 cut(s)	307, 1502, 1540, 1642, 2260, 2454, 2467
SfcI	CTRYAG	1 cut(s)	76
Sfr274I	CTCGAG	1 cut(s)	41
SfuI	TTCGAA	1 cut(s)	1716
SinI	GGWCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
SlaI	CTCGAG	1 cut(s)	41
SmaI	CCCGGG	1 cut(s)	1977
SmiMI	CAYNNNNRTG	5 cut(s)	638, 719, 1140, 1287, 2570
SmlI	CTYRAG	3 cut(s)	41, 446, 1082
SmoI	CTYRAG	3 cut(s)	41, 446, 1082
SphI	GCATGC	2 cut(s)	1178, 1182
SsiI	CCGC	8 cut(s)	5, 8, 57, 147, 1559, 1592, 1943, 2390
SspI	AATATT	1 cut(s)	2185
SspMI	CTAG	4 cut(s)	255, 302, 881, 1335
StyD4I	CCNGG	7 cut(s)	526, 730, 1423, 1910, 1974, 1975, 2551
StyI	CCWWGG	3 cut(s)	565, 930, 1400
TaiI	ACGT	3 cut(s)	2104, 2611, 2667
TatI	WGTACW	1 cut(s)	1068
TauI	GCSGC	5 cut(s)	8, 11, 60, 1562, 1595
TscAI	CASTG	4 cut(s)	432, 2251, 2539, 2574
TseFI	GTSAC	4 cut(s)	775, 876, 955, 2572
TseI	GCWGC	8 cut(s)	115, 1301, 1352, 1562, 1589, 2081, 2273, 2357
Tsp45I	GTSAC	4 cut(s)	775, 876, 955, 2572
TspDTI	ATGAA	9 cut(s)	338, 533, 1113, 1175, 1484, 1800, 1860, 1953, 2584
TspGWI	ACGGA	4 cut(s)	6, 663, 1626, 2251
TspMI	CCCGGG	1 cut(s)	1975
TspRI	CASTG	4 cut(s)	432, 2251, 2539, 2574
Tth111I	GACNNNGTC	3 cut(s)	998, 1966, 2553
VpaK11BI	GGWCC	6 cut(s)	364, 724, 1121, 1915, 2222, 2366
XagI	CCTNNNNNAGG	2 cut(s)	266, 531
XapI	RAATTY	4 cut(s)	62, 1247, 1531, 1615
XceI	RCATGY	4 cut(s)	514, 910, 1178, 1182
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2092, 2243
XhoI	CTCGAG	1 cut(s)	41
XmaI	CCCGGG	1 cut(s)	1975
XspI	CTAG	4 cut(s)	255, 302, 881, 1335
Zsp2I	ATGCAT	2 cut(s)	1115, 1180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.