Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Reverse (-)

10358687 .. 10364907

Gene Structure 6221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG124600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGGCGGCGGAGAATCCGTTCAAGAGTATATTGAAATCGCTTGAGAAGCCGGACGGCGGCGAGTTCGGAAAGTACTACAGCCTGCCGGCTCTCAATGATCCTAGAGTCGATAAGCTACCGTACTCTATTAAGATCCTTCTCGAGTCTGCAATCCGTAACTGCGATGAGTTTCAGGTTAAGAGTAAGGATGTTGAGAAAATTATTGATTGGGAGAACAGTGCTCCCAAGCAGGTTGAGATTCCATTCAAACCTGCTAGAGTCCTTCTTCAGGATTTCACTGGTGTACCTGCTGTTGTCGATCTGGCTTGCATGCGAGATGCCATGAACAATCTCAAGGGAGATTCTAACAAGATTAACCCATTGGTACCAGTTGATCTCGTCATTGATCACTCGGTTCAAGTAGATGTGGCAAGATCAGACAATGCAGTGCAGGCAAACATGGAATTTGAGTTCAAGAGAAACAATGAACGGTTTGGGTTTCTAAAATGGGGATCAAATGCATTCCATAACATGCTCGTTGTTCCTCCTGGATCAGGGATCGTTCATCAGGTTAATTTAGAATACCTTGGACGGGTGGTGTTCAACACAGATGGCTTGCTATATCCTGATAGTGTTGTTGGGACAGATTCACACACCACAATGATTGACGGACTAGGTGTTGCTGGTTGGGGAGTGGGTGGTATCGAAGCAGAAGCTGCGATGCTTGGCCAGCCCATGAGCATGGTCCTCCCTGGTGTGGTTGGGTTCAAGTTGTTAGGAAAACTGAGAGATGGTGTGACAGCTACTGATTTGGTTCTGACAGTAACTCAAATTCTGAGGAAGCATGGAGTTGTTGGGAAGTTTGTGGAGTTTTATGGGGAAGGCATGAGTGAATTATCATTAGCTGACCGTGCTACTATTGCCAACATGTCTCCTGAGTATGGTGCAACCATGGGCTTCTTTCCTGTCGATCATGTCACGCTGCAATATCTAAAGCTAACAGGCAGAACTGCTGACACTGTTGCTATGATAGAATCTTATTTACGGGCTAATAAGATGTTTGTGGACTACAGTGAGCCCCAGATAGAGAGAGTATACTCTGCTAATCTTGAGCTAAAACTTGAGGATGTGGAGCCTTGCATATCAGGGCCAAAGAGGCCACATGATCGTGTTCCTCTGAAAGAAATGAAAGTGGATTGGCATGCATGCCTTGACAATAGAGTTGGATTTAAGGGTTTTGCTGTGCCAAAGGAATCTCAAAAGAAAGTTGTAGAGTTCTCATTTCATGGAATCCCTGCACAGCTTAGGCATGGAGATGTCGTAATTGCAGCTATTACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTAGGAGCTGCCTTGGTTGCAAAGAAAGCTTGTGAATTAGGATTGGAGGTCAAGCCATGGATTAAGACTAGTCTTGCGCCGGGCTCTGGGGTTGTAACCAAATACTTGGAAAAGAGTGGGTTGCAGAAGTATCTGAATCAGCTAGGATTTAATATCGTTGGGTATGGATGCACCACATGCATTGGAAATTCAGGAGATATTGATGAAGCAGTGGCATCAGCTATTACGGAAAATGATATTGTAGCTTCAGCCGTATTATCTGGAAATAGGAATTTTGAGGGCCGTGTACATCCTTTAACACGGGCTAATTATCTTGCTTCTCCTCCCCTTGTGGTTGCTTATGCTCTTGCTGGCACGGTGGATATTGATTTTGACACTGAACCTATAGGGGTGGGAAAAGATGGAAAGAAGATATTTTTCAGGGATATATGGCCATCTAATCAAGAAGTAGCAGAGGCTGTGCAATCAAATGTGCTTCCTCAGATGTTTATGGCTACATATGAAGCAATCACCAAAGGAAATCCCATGTGGAATCAGTTATCTGTACCTTCTGGCACTCTCTATGCATGGGACCCAAAGTCCACTTACATACATGAGCCACCTTATTTTAAAGATATGACCATGTCTCCTCCCGGCGCTCATGGTGTGAAGAATGCTTACTGCTTGCTCAACTTTGGAGATAGTATTACAACTGACCACATCTCACCAGCTGGTAGCATCCACAAAGATAGTCCAGCAGCCAAATACCTTATGGAGCGTGGTGTTGATAGAAGAGACTTCAACTCTTATGGTAGTCGTCGTGGTAATGACGAAATAATGGCAAGAGGTACTTTTGCAAATATTCGCCTGGTCAATAAGTTTTTGATAGGAGAAGTGGGCCCCAAAACAATTCATCATCCCTCAGGAGAGAAAGTTTCCGTGTTTGATGCTGCCATGAAGTACAAAAGTGAAGGGCATGACACAATCATTTTGGCTGGTGCTGAATATGGGAGTGGAAGTTCTCGTGATTGGGCTGCTAAGGGTCCAATGCTACTGGGGGTGAAGGCTGTCATAGCAAAGAGCTTTGAGCGCATTCACCGCAGTAATCTTGTGGGCATGGGTATCATTCCCCTATGCTTCAAGGCTGGGGAGGATACTGAAACTCTTGGATTGACTGGTGAGGAACGCTACACCATCGACCTTCCAAGCACTGTTAGCGAAATTAGACCCGGTCAGGATATCACGGTGGTGACAGACAATGGAAAGTCATTTGTATGTACGCTACGGTTTGATACAGAGGTGGAGCTGGCTTATTTTGATCACGGAGGCATTTTGCAATATGTCATCAGGAATCTGATCGGCGCAAAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

900

Amino Acids

98.32

Weight (kDa)

5.86

Isoelectric Point (pI)

33.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	65 - 568	2.2e-167	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	697 - 825	2.2e-40	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	220, 259, 295
Acc65I	GGTACC	1 cut(s)	364
AccB1I	GGYRCC	1 cut(s)	364
AccI	GTMKAC	1 cut(s)	1074
AciI	CCGC	4 cut(s)	5, 8, 57, 2428
AclWI	GGATC	5 cut(s)	92, 127, 499, 538, 545
AcoI	YGGCCR	2 cut(s)	706, 1781
AcsI	RAATTY	4 cut(s)	443, 810, 1537, 1621
AcuI	CTGAAG	2 cut(s)	251, 1581
AfaI	GTAC	9 cut(s)	74, 122, 285, 366, 1638, 1896, 2179, 2291, 2608
AfiI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
AflIII	ACRYGT	1 cut(s)	906
AgsI	TTSAA	9 cut(s)	22, 34, 247, 398, 454, 583, 748, 2131, 2470
AhdI	GACNNNNNGTC	1 cut(s)	1927
AhlI	ACTAGT	1 cut(s)	1418
AjnI	CCWGG	3 cut(s)	526, 730, 2196
AleI	CACNNNNGTG	1 cut(s)	2576
AloI	GAACNNNNNNTCC	5 cut(s)	34, 434, 466, 2332, 2364
Alw21I	GWGCWC	1 cut(s)	223
Alw26I	GTCTC	3 cut(s)	915, 1980, 2118
AlwI	GGATC	5 cut(s)	92, 127, 499, 538, 545
AlwNI	CAGNNNCTG	3 cut(s)	695, 785, 1808
Ama87I	CYCGRG	1 cut(s)	140
AoxI	GGCC	6 cut(s)	706, 1127, 1136, 1630, 1781, 2227
ApaI	GGGCCC	1 cut(s)	2231
ApeKI	GCWGC	7 cut(s)	695, 961, 1307, 1358, 2087, 2279, 2363
ApoI	RAATTY	4 cut(s)	443, 810, 1537, 1621
Asp700I	GAANNNNTTC	1 cut(s)	17
Asp718I	GGTACC	1 cut(s)	364
AspLEI	GCGC	4 cut(s)	1429, 1988, 2421, 2693
AspS9I	GGNCC	7 cut(s)	724, 1127, 1630, 1921, 2227, 2228, 2372
AsuC2I	CCSGG	3 cut(s)	1431, 1983, 2559
AsuHPI	GGTGA	6 cut(s)	1852, 2046, 2401, 2417, 2519, 2590
AvaI	CYCGRG	1 cut(s)	140
AvaII	GGWCC	3 cut(s)	724, 1921, 2372
AxyI	CCTNAGG	1 cut(s)	2251
BaeGI	GKGCMC	1 cut(s)	2231
BaeI	ACNNNNGTAYC	4 cut(s)	267, 300, 2613, 2646
BalI	TGGCCA	2 cut(s)	708, 1783
BanI	GGYRCC	1 cut(s)	364
BanII	GRGCYC	3 cut(s)	1059, 1436, 2231
BarI	GAAGNNNNNNTAC	2 cut(s)	1991, 2023
BauI	CACGAG	1 cut(s)	2352
Bbv12I	GWGCWC	1 cut(s)	223
BbvI	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2099, 2266, 2350
BccI	CCATC	5 cut(s)	584, 764, 1745, 1792, 2531
BceAI	ACGGC	3 cut(s)	70, 1586, 1617
BciT130I	CCWGG	3 cut(s)	528, 732, 2198
BciVI	GTATCC	1 cut(s)	2476
BclI	TGATCA	2 cut(s)	385, 2647
BcnI	CCSGG	3 cut(s)	1431, 1983, 2559
BcoDI	GTCTC	3 cut(s)	915, 1980, 2118
BcuI	ACTAGT	1 cut(s)	1418
BfaI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1419, 1493
BfmI	CTRYAG	3 cut(s)	76, 1048, 1734
BfoI	RGCGCY	1 cut(s)	1989
BfuAI	ACCTGC	3 cut(s)	220, 259, 295
BfuI	GTATCC	1 cut(s)	2476
BglI	GCCNNNNNGGC	1 cut(s)	1135
BisI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
BlsI	GCNGC	9 cut(s)	7, 59, 697, 963, 1309, 1360, 2089, 2281, 2365
BmcAI	AGTACT	1 cut(s)	74
Bme1390I	CCNGG	6 cut(s)	528, 732, 1431, 1983, 2198, 2559
Bme18I	GGWCC	3 cut(s)	724, 1921, 2372
BmeRI	GACNNNNNGTC	1 cut(s)	1927
BmeT110I	CYCGRG	1 cut(s)	140
BmgT120I	GGNCC	7 cut(s)	724, 1127, 1630, 1921, 2227, 2228, 2372
BmiI	GGNNCC	7 cut(s)	366, 1113, 1922, 1923, 2229, 2230, 2373
BmrFI	CCNGG	6 cut(s)	528, 732, 1431, 1983, 2198, 2559
BmrI	ACTGGG	1 cut(s)	2393
BmsI	GCATC	6 cut(s)	307, 690, 1508, 1574, 2076, 2266
BmuI	ACTGGG	1 cut(s)	2393
Bpu10I	CCTNAGC	2 cut(s)	1283, 2367
BpuEI	CTTGAG	4 cut(s)	62, 317, 1109, 1121
BpuMI	CCSGG	3 cut(s)	1431, 1983, 2559
BsaJI	CCNNGG	5 cut(s)	565, 730, 930, 1362, 1406
BsaXI	ACNNNNNCTCC	9 cut(s)	32, 1284, 1314, 1960, 1990, 2247, 2277, 2332, 2362
Bsc4I	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
Bse118I	RCCGGY	1 cut(s)	85
Bse1I	ACTGG	5 cut(s)	283, 368, 1317, 2388, 2509
Bse21I	CCTNAGG	1 cut(s)	2251
BseBI	CCWGG	3 cut(s)	528, 732, 2198
BseDI	CCNNGG	5 cut(s)	565, 730, 930, 1362, 1406
BseGI	GGATG	6 cut(s)	193, 1111, 1523, 1639, 2067, 2245
BseLI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
BseMII	CTCAG	5 cut(s)	755, 806, 906, 1844, 2265
BseNI	ACTGG	5 cut(s)	283, 368, 1317, 2388, 2509
BseRI	GAGGAG	2 cut(s)	1662, 1968
BseSI	GKGCMC	1 cut(s)	2231
BseXI	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2099, 2266, 2350
BseYI	CCCAGC	1 cut(s)	2474
BsgI	GTGCAG	2 cut(s)	449, 1260
BshFI	GGCC	6 cut(s)	708, 1129, 1138, 1632, 1783, 2229
BshNI	GGYRCC	1 cut(s)	364
BsiHKAI	GWGCWC	1 cut(s)	223
BsiHKCI	CYCGRG	1 cut(s)	140
BsiSI	CCGG	5 cut(s)	50, 86, 1430, 1983, 2559
BslFI	GGGAC	2 cut(s)	634, 1934
BslI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
BsmAI	GTCTC	3 cut(s)	915, 1980, 2118
BsmFI	GGGAC	2 cut(s)	634, 1934
BsmI	GAATGC	3 cut(s)	500, 2008, 2421
BsnI	GGCC	6 cut(s)	708, 1129, 1138, 1632, 1783, 2229
BsoBI	CYCGRG	1 cut(s)	140
Bsp120I	GGGCCC	1 cut(s)	2227
Bsp1286I	GDGCHC	4 cut(s)	223, 1059, 1436, 2231
Bsp1407I	TGTACA	1 cut(s)	1636
Bsp19I	CCATGG	2 cut(s)	930, 1406
BspACI	CCGC	4 cut(s)	5, 8, 57, 2428
BspANI	GGCC	6 cut(s)	708, 1129, 1138, 1632, 1783, 2229
BspCNI	CTCAG	5 cut(s)	756, 807, 907, 1843, 2264
BspLI	GGNNCC	7 cut(s)	366, 1113, 1922, 1923, 2229, 2230, 2373
BspMI	ACCTGC	3 cut(s)	220, 259, 295
BspPI	GGATC	5 cut(s)	92, 127, 499, 538, 545
BspT107I	GGYRCC	1 cut(s)	364
BsrFI	RCCGGY	1 cut(s)	85
BsrGI	TGTACA	1 cut(s)	1636
BsrI	ACTGG	5 cut(s)	283, 368, 1317, 2388, 2509
BssAI	RCCGGY	1 cut(s)	85
BssECI	CCNNGG	5 cut(s)	565, 730, 930, 1362, 1406
BssNAI	GTATAC	1 cut(s)	1075
BssSI	CACGAG	1 cut(s)	2352
BssT1I	CCWWGG	4 cut(s)	565, 930, 1362, 1406
Bst1107I	GTATAC	1 cut(s)	1075
Bst2BI	CACGAG	1 cut(s)	2352
Bst2UI	CCWGG	3 cut(s)	528, 732, 2198
Bst6I	CTCTTC	1 cut(s)	2116
BstAPI	GCANNNNNTGC	2 cut(s)	695, 1527
BstAUI	TGTACA	1 cut(s)	1636
BstDEI	CTNAG	7 cut(s)	764, 815, 915, 1283, 1830, 2251, 2367
BstDSI	CCRYGG	2 cut(s)	930, 1406
BstENI	CCTNNNNNAGG	2 cut(s)	266, 531
BstF5I	GGATG	6 cut(s)	193, 1111, 1523, 1639, 2067, 2245
BstH2I	RGCGCY	1 cut(s)	1989
BstHHI	GCGC	4 cut(s)	1429, 1988, 2421, 2693
BstMAI	GTCTC	3 cut(s)	915, 1980, 2118
BstNI	CCWGG	3 cut(s)	528, 732, 2198
BstNSI	RCATGY	6 cut(s)	313, 514, 910, 1184, 1188, 1530
BstSCI	CCNGG	6 cut(s)	526, 730, 1429, 1981, 2196, 2557
BstSFI	CTRYAG	3 cut(s)	76, 1048, 1734
BstSLI	GKGCMC	1 cut(s)	2231
BstV1I	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2099, 2266, 2350
BstX2I	RGATCY	1 cut(s)	132
BstXI	CCANNNNNNTGG	2 cut(s)	721, 1456
BstYI	RGATCY	1 cut(s)	132
BstZ17I	GTATAC	1 cut(s)	1075
Bsu36I	CCTNAGG	1 cut(s)	2251
BsuI	GTATCC	1 cut(s)	2476
BsuRI	GGCC	6 cut(s)	708, 1129, 1138, 1632, 1783, 2229
BtgI	CCRYGG	2 cut(s)	930, 1406
BtgZI	GCGATG	2 cut(s)	177, 713
BtsCI	GGATG	6 cut(s)	193, 1111, 1523, 1639, 2067, 2245
BtsI	GCAGTG	2 cut(s)	432, 1566
BtsIMutI	CAGTG	8 cut(s)	223, 276, 432, 996, 1057, 1566, 1725, 2538
BveI	ACCTGC	3 cut(s)	220, 259, 295
CaiI	CAGNNNCTG	3 cut(s)	695, 785, 1808
CfoI	GCGC	4 cut(s)	1429, 1988, 2421, 2693
Cfr10I	RCCGGY	1 cut(s)	85
Cfr13I	GGNCC	7 cut(s)	724, 1127, 1630, 1921, 2227, 2228, 2372
Csp6I	GTAC	9 cut(s)	73, 121, 284, 365, 1637, 1895, 2178, 2290, 2607
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 1513, 1548
CviQI	GTAC	9 cut(s)	73, 121, 284, 365, 1637, 1895, 2178, 2290, 2607
DdeI	CTNAG	7 cut(s)	764, 815, 915, 1283, 1830, 2251, 2367
DraI	TTTAAA	1 cut(s)	1960
DriI	GACNNNNNGTC	1 cut(s)	1927
EaeI	YGGCCR	2 cut(s)	706, 1781
Eam1104I	CTCTTC	1 cut(s)	2116
Eam1105I	GACNNNNNGTC	1 cut(s)	1927
EarI	CTCTTC	1 cut(s)	2116
EciI	GGCGGA	1 cut(s)	23
Eco130I	CCWWGG	4 cut(s)	565, 930, 1362, 1406
Eco24I	GRGCYC	3 cut(s)	1059, 1436, 2231
Eco32I	GATATC	1 cut(s)	2569
Eco47I	GGWCC	3 cut(s)	724, 1921, 2372
Eco57I	CTGAAG	2 cut(s)	251, 1581
Eco81I	CCTNAGG	1 cut(s)	2251
Eco88I	CYCGRG	1 cut(s)	140
EcoNI	CCTNNNNNAGG	2 cut(s)	266, 531
EcoO109I	RGGNCCY	2 cut(s)	1921, 2228
EcoRII	CCWGG	3 cut(s)	526, 730, 2196
EcoRV	GATATC	1 cut(s)	2569
EcoT14I	CCWWGG	4 cut(s)	565, 930, 1362, 1406
EcoT22I	ATGCAT	4 cut(s)	502, 1186, 1532, 1918
EcoT38I	GRGCYC	3 cut(s)	1059, 1436, 2231
ErhI	CCWWGG	4 cut(s)	565, 930, 1362, 1406
FalI	AAGNNNNNCTT	2 cut(s)	2164, 2196
FaqI	GGGAC	2 cut(s)	634, 1934
FauNDI	CATATG	1 cut(s)	1849
FbaI	TGATCA	2 cut(s)	385, 2647
FblI	GTMKAC	1 cut(s)	1074
Fnu4HI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
FokI	GGATG	6 cut(s)	200, 1118, 1530, 1626, 2054, 2232
FriOI	GRGCYC	3 cut(s)	1059, 1436, 2231
Fsp4HI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
FspBI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1419, 1493
GlaI	GCGC	4 cut(s)	1428, 1987, 2420, 2692
GluI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
GsaI	CCCAGC	1 cut(s)	2478
HaeII	RGCGCY	1 cut(s)	1989
HaeIII	GGCC	6 cut(s)	708, 1129, 1138, 1632, 1783, 2229
HapII	CCGG	5 cut(s)	50, 86, 1430, 1983, 2559
HhaI	GCGC	4 cut(s)	1429, 1988, 2421, 2693
Hin6I	GCGC	4 cut(s)	1427, 1986, 2419, 2691
HinP1I	GCGC	4 cut(s)	1427, 1986, 2419, 2691
HindIII	AAGCTT	1 cut(s)	1377
HpaII	CCGG	5 cut(s)	50, 86, 1430, 1983, 2559
HphI	GGTGA	6 cut(s)	1852, 2046, 2401, 2417, 2519, 2590
Hpy166II	GTNNAC	5 cut(s)	284, 1045, 1075, 1637, 1932
Hpy188I	TCNGA	8 cut(s)	68, 418, 798, 816, 1158, 1485, 1833, 2685
Hpy8I	GTNNAC	5 cut(s)	284, 1045, 1075, 1637, 1932
Hpy99I	CGWCG	1 cut(s)	2151
HpyAV	CCTTC	7 cut(s)	146, 272, 854, 1908, 2294, 2386, 2540
HpyF3I	CTNAG	7 cut(s)	764, 815, 915, 1283, 1830, 2251, 2367
HspAI	GCGC	4 cut(s)	1427, 1986, 2419, 2691
KflI	GGGWCCC	1 cut(s)	1921
KpnI	GGTACC	1 cut(s)	368
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	2 cut(s)	385, 2647
LmnI	GCTCC	5 cut(s)	226, 1111, 1355, 2104, 2632
Lsp1109I	GCAGC	7 cut(s)	682, 948, 1319, 1345, 2099, 2266, 2350
LweI	GCATC	6 cut(s)	307, 690, 1508, 1574, 2076, 2266
MaeI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1419, 1493
MaeIII	GTNAC	6 cut(s)	155, 775, 802, 955, 1444, 2578
MboII	GAAGA	4 cut(s)	257, 1771, 2011, 2133
MflI	RGATCY	1 cut(s)	132
MhlI	GDGCHC	4 cut(s)	223, 1059, 1436, 2231
MlsI	TGGCCA	2 cut(s)	708, 1783
MluNI	TGGCCA	2 cut(s)	708, 1783
MlyI	GAGTC	3 cut(s)	114, 152, 267
MmeI	TCCRAC	1 cut(s)	1183
Mox20I	TGGCCA	2 cut(s)	708, 1783
Mph1103I	ATGCAT	4 cut(s)	502, 1186, 1532, 1918
MroNI	GCCGGC	1 cut(s)	85
MroXI	GAANNNNTTC	1 cut(s)	17
MscI	TGGCCA	2 cut(s)	708, 1783
MseI	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
MslI	CAYNNNNRTG	6 cut(s)	638, 719, 1146, 1293, 2576, 2602
Msp20I	TGGCCA	2 cut(s)	708, 1783
MspA1I	CMGCKG	1 cut(s)	2060
MspI	CCGG	5 cut(s)	50, 86, 1430, 1983, 2559
MspR9I	CCNGG	6 cut(s)	528, 732, 1431, 1983, 2198, 2559
Mva1269I	GAATGC	3 cut(s)	500, 2008, 2421
MvaI	CCWGG	3 cut(s)	528, 732, 2198
NaeI	GCCGGC	1 cut(s)	87
NciI	CCSGG	3 cut(s)	1431, 1983, 2559
NcoI	CCATGG	2 cut(s)	930, 1406
NdeI	CATATG	1 cut(s)	1849
NgoMIV	GCCGGC	1 cut(s)	85
NlaIV	GGNNCC	7 cut(s)	366, 1113, 1922, 1923, 2229, 2230, 2373
NmuCI	GTSAC	3 cut(s)	775, 955, 2578
NsiI	ATGCAT	4 cut(s)	502, 1186, 1532, 1918
NspI	RCATGY	6 cut(s)	313, 514, 910, 1184, 1188, 1530
OliI	CACNNNNGTG	1 cut(s)	2576
PaeI	GCATGC	3 cut(s)	313, 1184, 1188
PaeR7I	CTCGAG	1 cut(s)	140
PciI	ACATGT	1 cut(s)	906
PctI	GAATGC	3 cut(s)	500, 2008, 2421
PdiI	GCCGGC	1 cut(s)	87
PdmI	GAANNNNTTC	1 cut(s)	17
PflFI	GACNNNGTC	2 cut(s)	1972, 2559
PfoI	TCCNGGA	1 cut(s)	526
PkrI	GCNGC	9 cut(s)	7, 59, 697, 963, 1309, 1360, 2089, 2281, 2365
PleI	GAGTC	3 cut(s)	113, 151, 266
PpsI	GAGTC	3 cut(s)	113, 151, 266
PpuMI	RGGWCCY	1 cut(s)	1921
PscI	ACATGT	1 cut(s)	906
Psp5II	RGGWCCY	1 cut(s)	1921
Psp6I	CCWGG	3 cut(s)	526, 730, 2196
PspFI	CCCAGC	1 cut(s)	2474
PspGI	CCWGG	3 cut(s)	526, 730, 2196
PspN4I	GGNNCC	7 cut(s)	366, 1113, 1922, 1923, 2229, 2230, 2373
PspOMI	GGGCCC	1 cut(s)	2227
PspPI	GGNCC	7 cut(s)	724, 1127, 1630, 1921, 2227, 2228, 2372
PspPPI	RGGWCCY	1 cut(s)	1921
PstNI	CAGNNNCTG	3 cut(s)	695, 785, 1808
PsuI	RGATCY	1 cut(s)	132
PsyI	GACNNNGTC	2 cut(s)	1972, 2559
PvuII	CAGCTG	1 cut(s)	2060
RsaI	GTAC	9 cut(s)	74, 122, 285, 366, 1638, 1896, 2179, 2291, 2608
RsaNI	GTAC	9 cut(s)	73, 121, 284, 365, 1637, 1895, 2178, 2290, 2607
RseI	CAYNNNNRTG	6 cut(s)	638, 719, 1146, 1293, 2576, 2602
SaqAI	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
SatI	GCNGC	9 cut(s)	6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
Sau96I	GGNCC	7 cut(s)	724, 1127, 1630, 1921, 2227, 2228, 2372
ScaI	AGTACT	1 cut(s)	74
SchI	GAGTC	3 cut(s)	114, 152, 267
ScrFI	CCNGG	6 cut(s)	528, 732, 1431, 1983, 2198, 2559
SduI	GDGCHC	4 cut(s)	223, 1059, 1436, 2231
SfaNI	GCATC	6 cut(s)	307, 690, 1508, 1574, 2076, 2266
SfcI	CTRYAG	3 cut(s)	76, 1048, 1734
SfiI	GGCCNNNNNGGCC	1 cut(s)	1135
Sfr274I	CTCGAG	1 cut(s)	140
SinI	GGWCC	3 cut(s)	724, 1921, 2372
SlaI	CTCGAG	1 cut(s)	140
SmiMI	CAYNNNNRTG	6 cut(s)	638, 719, 1146, 1293, 2576, 2602
SmlI	CTYRAG	5 cut(s)	41, 140, 332, 1088, 1100
SmoI	CTYRAG	5 cut(s)	41, 140, 332, 1088, 1100
SpeI	ACTAGT	1 cut(s)	1418
SphI	GCATGC	3 cut(s)	313, 1184, 1188
SsiI	CCGC	4 cut(s)	5, 8, 57, 2428
SspI	AATATT	1 cut(s)	2191
SspMI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1419, 1493
StyD4I	CCNGG	6 cut(s)	526, 730, 1429, 1981, 2196, 2557
StyI	CCWWGG	4 cut(s)	565, 930, 1362, 1406
TaqI	TCGA	6 cut(s)	108, 141, 297, 684, 948, 2526
TatI	WGTACW	3 cut(s)	72, 1636, 2289
TauI	GCSGC	2 cut(s)	8, 60
Tru1I	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
Tru9I	TTAA	9 cut(s)	129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
TscAI	CASTG	8 cut(s)	223, 283, 432, 1003, 1057, 1566, 1732, 2545
TseFI	GTSAC	3 cut(s)	775, 955, 2578
TseI	GCWGC	7 cut(s)	695, 961, 1307, 1358, 2087, 2279, 2363
Tsp45I	GTSAC	3 cut(s)	775, 955, 2578
TspDTI	ATGAA	9 cut(s)	338, 480, 533, 1181, 1253, 1569, 1866, 2231, 2300
TspGWI	ACGGA	6 cut(s)	6, 143, 663, 1592, 2257, 2667
TspRI	CASTG	8 cut(s)	223, 283, 432, 1003, 1057, 1566, 1732, 2545
Tth111I	GACNNNGTC	2 cut(s)	1972, 2559
VpaK11BI	GGWCC	3 cut(s)	724, 1921, 2372
XagI	CCTNNNNNAGG	2 cut(s)	266, 531
XapI	RAATTY	4 cut(s)	443, 810, 1537, 1621
XceI	RCATGY	6 cut(s)	313, 514, 910, 1184, 1188, 1530
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2098
XhoI	CTCGAG	1 cut(s)	140
XmiI	GTMKAC	1 cut(s)	1074
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	7 cut(s)	102, 255, 653, 1341, 1352, 1419, 1493
ZrmI	AGTACT	1 cut(s)	74
Zsp2I	ATGCAT	4 cut(s)	502, 1186, 1532, 1918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.