Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Reverse (-)

10055419 .. 10067646

Gene Structure 12228 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1123700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2697 bp

ATGGCTGCCGCGAATCCGTTTCAGAAGATACTGACGGCGCTCGAGAAGCCGGACGGCGGCGAATTCGGCAAATACTACAGCTTGCCGGCTCTCAACGATCCCAGAATCGAGAAGCTGCCATTCTCGATTAAGATCCTTCTCGAATCCGCTATTCGTAATTGTGATGAGTTTCAAGTGAAGAGTAATGATGTTGAGAAAATTATTGATTGGGAGAACACTTCTCCCAAGCAGGTTGAGATCCCATTCAAACCTGCTAGAGTCCTGCTTCAGGACTTTACTGGAGTACCAGCTGTCGTTGATCTAGCTTGTATGCGTGATGCCATGAACAATCTGAAGGGGGATTCTAACAAGATTAACCCATTGGTCCCAGTTGATCTTGTCATTGATCACTCGGTTCAAGTAGATGTTGCAAGATCAGAAAATGCCGTGCAGGCAAATATGGAATTTGAGTTCCAGCGAAACAAAGAACGGTTTGGTTTTCTGAAGTGGGGATCAGATGCATTCCATAACATGCTTGTTGTTCCTCCTGGATCAGGGATTGTTCACCAGGTTAATTTGGAATACCTTGGTCGAGTTGTGTTTAACACAGACGGCTTGCTTTATCCTGATAGTGTTGTCGGGACAGATTCACACACAACTATGATAGATGGATTAGGTGTCGCTGGCTGGGGAGTAGGTGGTATCGAAGCAGAAGCCACTATGCTTGGCCAGCCCATGAGCATGGTCCTGCCTGGTGTTGTTGGGTTTAAATTATTAGGAAAACTGAGAGACGGTGTGACAGCTACTGACTTGGTTCTTACAGTAACTCAAATGCTGAGGAAGCATGGAGTTGTTGGCAAGTTTGTGGAGTTCTATGGGGAAGGCATGCGTGAATTGTCACTAGCAGACCGTGCCACTATTGCCAACATGTCTCCCGAGTATGGTGCAACCATGGGCTTCTTCCCAGTGGATCATGTCACCCTGCAGTATCTAAAACTTACGGGCAGAAGTGATGACAAGGTCGCTCTGATAGAATCCTATTTACGGGCTAATAAGATGTTTGTCGACTACAATGAGCCCCAGATTGAGAGAGTGTACTCCTCCTATCTTGAGTTAAATCTTAATGATGTGGAACCATGCATATCCGGTCCAAAGAGGCCACACGATCGTGTTACTTTGAAAGAAATGAAAGGGGATTGGCATGCATGCCTTGACAATAGAGTTGGATTTAAGGGTTTTGCTGTGCCAGAGGAATCTCAAAGTAAGGTTGTAGAATTCAATTTCCACGGAACTCCAGCACAGCTTAGGCATGGGGATGTTGTTATTGCAGCTATCACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTTGGAGCTGCTTTGGTTGCCAAGAAAGCTTGTGAATTAGGATTGGAGGTCAAGCCATGGATTAAGACAAGTCTTGCTCCTGGTTCTGGGGTTGTAACTAAATACTTGCAAAAGAGTGGGTTGCAGAAGTATTTGAATCAGTTAGGATTTCATATTGTTGGGTATGGATGCACGACATGCATTGGGAATTCAGGAGATATTGATGATGCAGTAGCGGCTGCTATTACAGAAAATGATCTTGTAGCTGCGGCTGTATTATCTGGAAATAGGAATTTTGAGGGTCGTGTGCATCCGTTAACAAGGGCTAATTATCTTGCTTCTCCTCCCCTTGTTGTTGCCTATGCTCTTGCTGGAACGGTAGATATTGATTTTGAAAAAGAACCAATTGGGGTGGGAAAAGATGGAAAGAAGATATTTTTCAGGGACATCTGGCCATCCAATGAAGAAGTGGCAACAGTTGTGCAATCAAATGTGCTTCCTCATATGTTTATGGCTACATATGAAGCAATCACCAAGGGAAATCCAATGTGGAATCAGTTATCTGTACCATCTGGCACTCTTTATGCCTGGGACCCAAAATCAACATATATACATGAACCGCCGTACTTTAAGGATATGACCATGTCTCCTCCGGGTGCTCATGGTGTGAAGAATGCTTACTGCTTGCTCAACTTTGGAGATAGTATTACAACTGACCACATCTCACCAGCTGGTAGCATCCACAAAGACAGCCCAGCAGCTAAATACCTTCTGGAACGTGGTGTTGATAGAAGAGACTTCAACTCTTATGGAAGTCGCCGTGGTAATGACGAGATTATGGCAAGAGGGACTTTTGCTAATATTCGGCTGGTCAATAAGTTTTTGAAAGGAGAAGTTGGTCCCAAAACAATCCACATTCCCACCGGAGAGAAACTGTCTGTGTTTGATGCTGCTATGAGGTACAAAAGCGAAGGACATGATACGATTATTTTGGCTGGTGCTGAGTATGGAAGTGGAAGTTCTCGTGATTGGGCTGCAAAGGGTCCAATGCTATTGGGTGTGAAGGCAGTCATATCAAAGAGCTTTGAGCGCATTCACCGTAGTAATCTTGTTGGCATGGGCATCATTCCGCTATGTTTCAAGACCGGGGAGGATGCTGACTCTCTTGGTTTAACCGGCGAGGAACGCTACACCATCAATCTTCCCAGCACTGTCAGTGAAATTAGACCCGGTCAGGATGTCACAGTGGTGACAGATAATGGCAAGTCCTTCGTATGTACACTACGTTTCGATACAGAGGTTGAGCTGGCATATTTCGATCACGGAGGCATTTTGCAGTATGTCATCAGGAATCTGATCAAAAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

899

Amino Acids

98.37

Weight (kDa)

5.89

Isoelectric Point (pI)

35.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	65 - 568	2.8e-168	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	697 - 824	1.9e-40	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	220, 259
AccI	GTMKAC	1 cut(s)	1044
AccII	CGCG	1 cut(s)	11
AciI	CCGC	7 cut(s)	9, 57, 147, 1565, 1598, 1949, 2459
AclWI	GGATC	6 cut(s)	92, 127, 232, 499, 538, 957
AcoI	YGGCCR	2 cut(s)	706, 1781
AcsI	RAATTY	5 cut(s)	62, 443, 1253, 1537, 1621
AcuI	CTGAAG	3 cut(s)	251, 353, 503
AfaI	GTAC	6 cut(s)	285, 1076, 1896, 1955, 2291, 2608
AfiI	CCNNNNNNNGG	6 cut(s)	56, 268, 533, 920, 1023, 1436
AflIII	ACRYGT	1 cut(s)	906
AjnI	CCWGG	5 cut(s)	526, 546, 730, 1429, 1916
AleI	CACNNNNGTG	2 cut(s)	1146, 2576
AloI	GAACNNNNNNTCC	2 cut(s)	434, 466
Alw21I	GWGCWC	1 cut(s)	1990
Alw26I	GTCTC	4 cut(s)	762, 915, 1980, 2118
AlwI	GGATC	6 cut(s)	92, 127, 232, 499, 538, 957
AlwNI	CAGNNNCTG	1 cut(s)	785
Ama87I	CYCGRG	2 cut(s)	41, 914
AoxI	GGCC	3 cut(s)	706, 1136, 1781
ApeKI	GCWGC	9 cut(s)	5, 115, 1307, 1358, 1568, 1595, 2087, 2279, 2363
ApoI	RAATTY	5 cut(s)	62, 443, 1253, 1537, 1621
AspLEI	GCGC	2 cut(s)	40, 2421
AspS9I	GGNCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
AsuC2I	CCSGG	3 cut(s)	1983, 2476, 2559
AsuHPI	GGTGA	7 cut(s)	536, 949, 1306, 1852, 2046, 2417, 2590
AvaI	CYCGRG	2 cut(s)	41, 914
AvaII	GGWCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
BaeI	ACNNNNGTAYC	4 cut(s)	267, 300, 2613, 2646
BalI	TGGCCA	2 cut(s)	708, 1783
BanII	GRGCYC	1 cut(s)	1059
BarI	GAAGNNNNNNTAC	2 cut(s)	1991, 2023
BauI	CACGAG	1 cut(s)	2352
Bbv12I	GWGCWC	1 cut(s)	1990
BbvCI	CCTCAGC	1 cut(s)	815
BbvI	GCAGC	8 cut(s)	102, 1319, 1345, 1555, 1582, 2099, 2266, 2350
BccI	CCATC	5 cut(s)	641, 1745, 1792, 1906, 2531
BceAI	ACGGC	6 cut(s)	51, 70, 410, 607, 1936, 2133
BcgI	CGANNNNNNTGC	4 cut(s)	905, 939, 2581, 2615
BciT130I	CCWGG	5 cut(s)	528, 548, 732, 1431, 1918
BclI	TGATCA	2 cut(s)	385, 2685
BcnI	CCSGG	3 cut(s)	1983, 2476, 2559
BcoDI	GTCTC	4 cut(s)	762, 915, 1980, 2118
BfaI	CTAG	5 cut(s)	255, 302, 881, 1341, 2695
BfmI	CTRYAG	2 cut(s)	76, 962
BfoI	RGCGCY	1 cut(s)	41
BfuAI	ACCTGC	2 cut(s)	220, 259
BglI	GCCNNNNNGGC	1 cut(s)	431
Bme1390I	CCNGG	8 cut(s)	528, 548, 732, 1431, 1918, 1983, 2476, 2559
Bme18I	GGWCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
BmeT110I	CYCGRG	2 cut(s)	41, 914
BmgT120I	GGNCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
BmiI	GGNNCC	6 cut(s)	366, 1113, 1922, 1923, 2230, 2373
BmrFI	CCNGG	8 cut(s)	528, 548, 732, 1431, 1918, 1983, 2476, 2559
BmrI	ACTGGG	2 cut(s)	362, 938
BmsI	GCATC	9 cut(s)	307, 487, 1508, 1546, 1648, 2076, 2266, 2460, 2473
BmuI	ACTGGG	2 cut(s)	362, 938
BpmI	CTGGAG	2 cut(s)	300, 1257
Bpu10I	CCTNAGC	2 cut(s)	815, 1283
BpuEI	CTTGAG	1 cut(s)	1109
BpuMI	CCSGG	3 cut(s)	1983, 2476, 2559
BsaBI	GATNNNNATC	1 cut(s)	131
BsaJI	CCNNGG	8 cut(s)	565, 930, 1264, 1406, 1863, 1917, 2149, 2475
BsaWI	WCCGGW	2 cut(s)	1124, 2252
BsaXI	ACNNNNNCTCC	2 cut(s)	1960, 1990
Bsc4I	CCNNNNNNNGG	6 cut(s)	56, 268, 533, 920, 1023, 1436
Bse118I	RCCGGY	2 cut(s)	85, 2504
Bse1I	ACTGG	4 cut(s)	283, 368, 944, 1317
Bse8I	GATNNNNATC	1 cut(s)	131
BseBI	CCWGG	5 cut(s)	528, 548, 732, 1431, 1918
BseDI	CCNNGG	8 cut(s)	565, 930, 1264, 1406, 1863, 1917, 2149, 2475
BseGI	GGATG	7 cut(s)	1300, 1523, 1639, 1784, 2067, 2488, 2572
BseJI	GATNNNNATC	1 cut(s)	131
BseLI	CCNNNNNNNGG	6 cut(s)	56, 268, 533, 920, 1023, 1436
BseMII	CTCAG	3 cut(s)	755, 806, 2322
BseNI	ACTGG	4 cut(s)	283, 368, 944, 1317
BseRI	GAGGAG	3 cut(s)	1069, 1662, 1968
BseXI	GCAGC	8 cut(s)	102, 1319, 1345, 1555, 1582, 2099, 2266, 2350
BseYI	CCCAGC	3 cut(s)	666, 2083, 2534
BsgI	GTGCAG	1 cut(s)	449
Bsh1236I	CGCG	1 cut(s)	11
Bsh1285I	CGRYCG	1 cut(s)	1147
BshFI	GGCC	3 cut(s)	708, 1138, 1783
BsiEI	CGRYCG	1 cut(s)	1147
BsiHKAI	GWGCWC	1 cut(s)	1990
BsiHKCI	CYCGRG	2 cut(s)	41, 914
BsiSI	CCGG	8 cut(s)	50, 86, 1125, 1982, 2253, 2475, 2505, 2559
BslFI	GGGAC	6 cut(s)	350, 634, 1787, 1934, 2191, 2214
BslI	CCNNNNNNNGG	6 cut(s)	56, 268, 533, 920, 1023, 1436
BsmAI	GTCTC	4 cut(s)	762, 915, 1980, 2118
BsmBI	CGTCTC	1 cut(s)	762
BsmFI	GGGAC	6 cut(s)	350, 634, 1787, 1934, 2191, 2214
BsmI	GAATGC	3 cut(s)	500, 2008, 2421
BsnI	GGCC	3 cut(s)	708, 1138, 1783
BsoBI	CYCGRG	2 cut(s)	41, 914
Bsp1286I	GDGCHC	2 cut(s)	1059, 1990
Bsp1407I	TGTACA	1 cut(s)	2606
Bsp19I	CCATGG	2 cut(s)	930, 1406
BspACI	CCGC	7 cut(s)	9, 57, 147, 1565, 1598, 1949, 2459
BspANI	GGCC	3 cut(s)	708, 1138, 1783
BspCNI	CTCAG	3 cut(s)	756, 807, 2323
BspFNI	CGCG	1 cut(s)	11
BspLI	GGNNCC	6 cut(s)	366, 1113, 1922, 1923, 2230, 2373
BspMAI	CTGCAG	1 cut(s)	966
BspMI	ACCTGC	2 cut(s)	220, 259
BspPI	GGATC	6 cut(s)	92, 127, 232, 499, 538, 957
BsrFI	RCCGGY	2 cut(s)	85, 2504
BsrGI	TGTACA	1 cut(s)	2606
BsrI	ACTGG	4 cut(s)	283, 368, 944, 1317
BssAI	RCCGGY	2 cut(s)	85, 2504
BssECI	CCNNGG	8 cut(s)	565, 930, 1264, 1406, 1863, 1917, 2149, 2475
BssSI	CACGAG	1 cut(s)	2352
BssT1I	CCWWGG	4 cut(s)	565, 930, 1406, 1863
Bst2BI	CACGAG	1 cut(s)	2352
Bst2UI	CCWGG	5 cut(s)	528, 548, 732, 1431, 1918
Bst6I	CTCTTC	2 cut(s)	173, 2116
BstAPI	GCANNNNNTGC	2 cut(s)	890, 1527
BstAUI	TGTACA	1 cut(s)	2606
BstDEI	CTNAG	4 cut(s)	764, 815, 1283, 2331
BstDSI	CCRYGG	4 cut(s)	930, 1264, 1406, 2149
BstENI	CCTNNNNNAGG	2 cut(s)	266, 531
BstF5I	GGATG	7 cut(s)	1300, 1523, 1639, 1784, 2067, 2488, 2572
BstFNI	CGCG	1 cut(s)	11
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	2 cut(s)	40, 2421
BstMAI	GTCTC	4 cut(s)	762, 915, 1980, 2118
BstMCI	CGRYCG	1 cut(s)	1147
BstNI	CCWGG	5 cut(s)	528, 548, 732, 1431, 1918
BstNSI	RCATGY	6 cut(s)	514, 868, 910, 1184, 1188, 1530
BstSCI	CCNGG	8 cut(s)	526, 546, 730, 1429, 1916, 1981, 2474, 2557
BstSFI	CTRYAG	2 cut(s)	76, 962
BstUI	CGCG	1 cut(s)	11
BstV1I	GCAGC	8 cut(s)	102, 1319, 1345, 1555, 1582, 2099, 2266, 2350
BstX2I	RGATCY	2 cut(s)	132, 237
BstXI	CCANNNNNNTGG	1 cut(s)	721
BstYI	RGATCY	2 cut(s)	132, 237
BsuRI	GGCC	3 cut(s)	708, 1138, 1783
BtgI	CCRYGG	4 cut(s)	930, 1264, 1406, 2149
BtsCI	GGATG	7 cut(s)	1300, 1523, 1639, 1784, 2067, 2488, 2572
BtsIMutI	CAGTG	4 cut(s)	951, 2538, 2551, 2580
BveI	ACCTGC	2 cut(s)	220, 259
CaiI	CAGNNNCTG	1 cut(s)	785
CfoI	GCGC	2 cut(s)	40, 2421
Cfr10I	RCCGGY	2 cut(s)	85, 2504
Cfr13I	GGNCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
CsiI	ACCWGGT	1 cut(s)	546
Csp6I	GTAC	6 cut(s)	284, 1075, 1895, 1954, 2290, 2607
CspCI	CAANNNNNGTGG	4 cut(s)	685, 720, 1722, 1757
CviQI	GTAC	6 cut(s)	284, 1075, 1895, 1954, 2290, 2607
DdeI	CTNAG	4 cut(s)	764, 815, 1283, 2331
DraI	TTTAAA	1 cut(s)	748
EaeI	YGGCCR	2 cut(s)	706, 1781
Eam1104I	CTCTTC	2 cut(s)	173, 2116
EarI	CTCTTC	2 cut(s)	173, 2116
Eco130I	CCWWGG	4 cut(s)	565, 930, 1406, 1863
Eco24I	GRGCYC	1 cut(s)	1059
Eco47I	GGWCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
Eco57I	CTGAAG	3 cut(s)	251, 353, 503
Eco88I	CYCGRG	2 cut(s)	41, 914
EcoNI	CCTNNNNNAGG	2 cut(s)	266, 531
EcoO109I	RGGNCCY	1 cut(s)	1921
EcoRI	GAATTC	3 cut(s)	62, 1253, 1537
EcoRII	CCWGG	5 cut(s)	526, 546, 730, 1429, 1916
EcoT14I	CCWWGG	4 cut(s)	565, 930, 1406, 1863
EcoT22I	ATGCAT	4 cut(s)	502, 1121, 1186, 1532
EcoT38I	GRGCYC	1 cut(s)	1059
ErhI	CCWWGG	4 cut(s)	565, 930, 1406, 1863
Esp3I	CGTCTC	1 cut(s)	762
FalI	AAGNNNNNCTT	2 cut(s)	2164, 2196
FaqI	GGGAC	6 cut(s)	350, 634, 1787, 1934, 2191, 2214
FauNDI	CATATG	2 cut(s)	1833, 1849
FbaI	TGATCA	2 cut(s)	385, 2685
FblI	GTMKAC	1 cut(s)	1044
FokI	GGATG	7 cut(s)	1307, 1530, 1626, 1771, 2054, 2495, 2579
FriOI	GRGCYC	1 cut(s)	1059
FspBI	CTAG	5 cut(s)	255, 302, 881, 1341, 2695
GlaI	GCGC	2 cut(s)	39, 2420
GsaI	CCCAGC	3 cut(s)	670, 2087, 2538
GsuI	CTGGAG	2 cut(s)	300, 1257
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	3 cut(s)	708, 1138, 1783
HapII	CCGG	8 cut(s)	50, 86, 1125, 1982, 2253, 2475, 2505, 2559
HhaI	GCGC	2 cut(s)	40, 2421
Hin6I	GCGC	2 cut(s)	38, 2419
HinP1I	GCGC	2 cut(s)	38, 2419
HincII	GTYRAC	2 cut(s)	1045, 1647
HindII	GTYRAC	2 cut(s)	1045, 1647
HindIII	AAGCTT	1 cut(s)	1377
HpaI	GTTAAC	1 cut(s)	1647
HpaII	CCGG	8 cut(s)	50, 86, 1125, 1982, 2253, 2475, 2505, 2559
HphI	GGTGA	7 cut(s)	536, 949, 1306, 1852, 2046, 2417, 2590
Hpy166II	GTNNAC	5 cut(s)	544, 1045, 1075, 1647, 2609
Hpy188I	TCNGA	7 cut(s)	24, 333, 418, 483, 496, 1008, 2685
Hpy8I	GTNNAC	5 cut(s)	544, 1045, 1075, 1647, 2609
HpyAV	CCTTC	7 cut(s)	146, 328, 854, 2108, 2294, 2386, 2608
HpyCH4IV	ACGT	2 cut(s)	2107, 2614
HpyF3I	CTNAG	4 cut(s)	764, 815, 1283, 2331
HpySE526I	ACGT	2 cut(s)	2107, 2614
HspAI	GCGC	2 cut(s)	38, 2419
KflI	GGGWCCC	1 cut(s)	1921
KroI	GCCGGC	1 cut(s)	85
KroNI	GCCGGC	1 cut(s)	87
Ksp22I	TGATCA	2 cut(s)	385, 2685
KspAI	GTTAAC	1 cut(s)	1647
LmnI	GCTCC	2 cut(s)	1355, 1432
Lsp1109I	GCAGC	8 cut(s)	102, 1319, 1345, 1555, 1582, 2099, 2266, 2350
LweI	GCATC	9 cut(s)	307, 487, 1508, 1546, 1648, 2076, 2266, 2460, 2473
MabI	ACCWGGT	1 cut(s)	546
MaeI	CTAG	5 cut(s)	255, 302, 881, 1341, 2695
MaeII	ACGT	2 cut(s)	2107, 2614
MaeIII	GTNAC	8 cut(s)	775, 802, 876, 955, 1150, 1444, 2569, 2578
MboII	GAAGA	8 cut(s)	37, 190, 931, 1771, 1805, 2011, 2133, 2522
MfeI	CAATTG	1 cut(s)	1734
MflI	RGATCY	2 cut(s)	132, 237
MhlI	GDGCHC	2 cut(s)	1059, 1990
MlsI	TGGCCA	2 cut(s)	708, 1783
MluNI	TGGCCA	2 cut(s)	708, 1783
MlyI	GAGTC	2 cut(s)	267, 2483
MmeI	TCCRAC	1 cut(s)	1183
Mox20I	TGGCCA	2 cut(s)	708, 1783
Mph1103I	ATGCAT	4 cut(s)	502, 1121, 1186, 1532
MroNI	GCCGGC	1 cut(s)	85
MscI	TGGCCA	2 cut(s)	708, 1783
MslI	CAYNNNNRTG	5 cut(s)	638, 719, 1146, 1293, 2576
Msp20I	TGGCCA	2 cut(s)	708, 1783
MspA1I	CMGCKG	2 cut(s)	290, 2060
MspI	CCGG	8 cut(s)	50, 86, 1125, 1982, 2253, 2475, 2505, 2559
MspR9I	CCNGG	8 cut(s)	528, 548, 732, 1431, 1918, 1983, 2476, 2559
MunI	CAATTG	1 cut(s)	1734
Mva1269I	GAATGC	3 cut(s)	500, 2008, 2421
MvaI	CCWGG	5 cut(s)	528, 548, 732, 1431, 1918
MvnI	CGCG	1 cut(s)	11
NaeI	GCCGGC	1 cut(s)	87
NciI	CCSGG	3 cut(s)	1983, 2476, 2559
NcoI	CCATGG	2 cut(s)	930, 1406
NdeI	CATATG	2 cut(s)	1833, 1849
NgoMIV	GCCGGC	1 cut(s)	85
NlaIV	GGNNCC	6 cut(s)	366, 1113, 1922, 1923, 2230, 2373
NmuCI	GTSAC	5 cut(s)	775, 876, 955, 2569, 2578
NsiI	ATGCAT	4 cut(s)	502, 1121, 1186, 1532
NspI	RCATGY	6 cut(s)	514, 868, 910, 1184, 1188, 1530
OliI	CACNNNNGTG	2 cut(s)	1146, 2576
PaeI	GCATGC	3 cut(s)	868, 1184, 1188
PaeR7I	CTCGAG	1 cut(s)	41
PciI	ACATGT	1 cut(s)	906
PctI	GAATGC	3 cut(s)	500, 2008, 2421
PdiI	GCCGGC	1 cut(s)	87
PflFI	GACNNNGTC	3 cut(s)	998, 1972, 2559
PfoI	TCCNGGA	1 cut(s)	526
Ple19I	CGATCG	1 cut(s)	1147
PleI	GAGTC	2 cut(s)	266, 2483
PpsI	GAGTC	2 cut(s)	266, 2483
PpuMI	RGGWCCY	1 cut(s)	1921
PscI	ACATGT	1 cut(s)	906
Psp5II	RGGWCCY	1 cut(s)	1921
Psp6I	CCWGG	5 cut(s)	526, 546, 730, 1429, 1916
PspFI	CCCAGC	3 cut(s)	666, 2083, 2534
PspGI	CCWGG	5 cut(s)	526, 546, 730, 1429, 1916
PspN4I	GGNNCC	6 cut(s)	366, 1113, 1922, 1923, 2230, 2373
PspPI	GGNCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
PspPPI	RGGWCCY	1 cut(s)	1921
PstI	CTGCAG	1 cut(s)	966
PstNI	CAGNNNCTG	1 cut(s)	785
PsuI	RGATCY	2 cut(s)	132, 237
PsyI	GACNNNGTC	3 cut(s)	998, 1972, 2559
PvuI	CGATCG	1 cut(s)	1147
PvuII	CAGCTG	2 cut(s)	290, 2060
RsaI	GTAC	6 cut(s)	285, 1076, 1896, 1955, 2291, 2608
RsaNI	GTAC	6 cut(s)	284, 1075, 1895, 1954, 2290, 2607
RseI	CAYNNNNRTG	5 cut(s)	638, 719, 1146, 1293, 2576
SalI	GTCGAC	1 cut(s)	1043
Sau96I	GGNCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
SchI	GAGTC	2 cut(s)	267, 2483
ScrFI	CCNGG	8 cut(s)	528, 548, 732, 1431, 1918, 1983, 2476, 2559
SduI	GDGCHC	2 cut(s)	1059, 1990
SexAI	ACCWGGT	1 cut(s)	546
SfaNI	GCATC	9 cut(s)	307, 487, 1508, 1546, 1648, 2076, 2266, 2460, 2473
SfcI	CTRYAG	2 cut(s)	76, 962
Sfr274I	CTCGAG	1 cut(s)	41
SinI	GGWCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
SlaI	CTCGAG	1 cut(s)	41
SmiMI	CAYNNNNRTG	5 cut(s)	638, 719, 1146, 1293, 2576
SmlI	CTYRAG	2 cut(s)	41, 1088
SmoI	CTYRAG	2 cut(s)	41, 1088
SphI	GCATGC	3 cut(s)	868, 1184, 1188
SsiI	CCGC	7 cut(s)	9, 57, 147, 1565, 1598, 1949, 2459
SspI	AATATT	1 cut(s)	2191
SspMI	CTAG	5 cut(s)	255, 302, 881, 1341, 2695
StyD4I	CCNGG	8 cut(s)	526, 546, 730, 1429, 1916, 1981, 2474, 2557
StyI	CCWWGG	4 cut(s)	565, 930, 1406, 1863
TaiI	ACGT	2 cut(s)	2110, 2617
TaqI	TCGA	9 cut(s)	42, 108, 125, 141, 571, 684, 1044, 2619, 2646
TatI	WGTACW	2 cut(s)	1074, 2606
TauI	GCSGC	4 cut(s)	11, 60, 1568, 1601
TscAI	CASTG	4 cut(s)	951, 2545, 2551, 2580
TseFI	GTSAC	5 cut(s)	775, 876, 955, 2569, 2578
TseI	GCWGC	9 cut(s)	5, 115, 1307, 1358, 1568, 1595, 2087, 2279, 2363
Tsp45I	GTSAC	5 cut(s)	775, 876, 955, 2569, 2578
TspDTI	ATGAA	6 cut(s)	338, 1181, 1490, 1806, 1866, 1959
TspGWI	ACGGA	4 cut(s)	6, 1281, 1632, 2667
TspRI	CASTG	4 cut(s)	951, 2545, 2551, 2580
Tth111I	GACNNNGTC	3 cut(s)	998, 1972, 2559
VpaK11BI	GGWCC	6 cut(s)	364, 724, 1127, 1921, 2228, 2372
XagI	CCTNNNNNAGG	2 cut(s)	266, 531
XapI	RAATTY	5 cut(s)	62, 443, 1253, 1537, 1621
XceI	RCATGY	6 cut(s)	514, 868, 910, 1184, 1188, 1530
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1795
XhoI	CTCGAG	1 cut(s)	41
XmiI	GTMKAC	1 cut(s)	1044
XspI	CTAG	5 cut(s)	255, 302, 881, 1341, 2695
Zsp2I	ATGCAT	4 cut(s)	502, 1121, 1186, 1532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.