Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0011733

Physical Location & Seq

Genomic Coordinates Reverse (-)

6054564 .. 6060614

Gene Structure 6051 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g11110.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2697 bp

ATGGCGGCGGAGAATCCGTTCAAGAGTATATTGAAATCGCTCGAGAAGCCGGACGGCGGCGAGTTCGGGAAGTACTACAGCTTGCCAGCTCTCAATGATCCTAGAGTCGATAAGCTACCGTACTCTATTAAGATCCTTCTGGAGTCTGCAATTCGCAACTGCGATGAGTTTCAGGTTAAGAGCAAGGATGTTGAGAAGATTATTGATTGGGAGAACAGTGCTCCCAAGCAGGTTGAGATCCCATTCAAACCTGCTAGAGTCCTTCTTCAGGATTTTACTGGTGTGCCAGCTGTGGTTGATCTAGCTTGCATGCGAGATGCCATGAACAATCTCAAGGGAGATTCTAACAAGATTAACCCATTGGTACCAGTAGATCTTGTCATTGATCACTCAGTTCAGGTAGATGTGGCAAGATCAGAAAATGCTGTGCAGGCAAATATGGAACTAGAGTTCCAGAGAAACAACGAACGGTTTGGTTTTCTGAAATGGGGATCAAATGCATTCGATAACATGCTTGTTGTTCCTCCTGGATCAGGAATTGTTCATCAGGTTAATTTAGAATACCTTGGGCGGGTGGTGTTCAACACAAATGGCTTGCTATATCCTGATAGTGTTGTTGGGACAGATTCACACACCACAATGATTGACGGATTAGGTGTTGCTGGTTGGGGAGTAGGTGGGATTGAAGCAGAAGCTACTATGCTTGGCCAGCCAATGAGCATGGTCCTCCCTGGTGTGGTTGGGTTCAAACTGTTAGGAAAATTGAGAGATGGTGTGACAGCTACCGACTTGGTTCTGACAGTAACTCAAATGCTGAGGAAGCATGGAGTTGTTGGGAAGTTTGTGGAGTTTTATGGGGAAGGCATGAGTGAATTGTCATTAGCTGACCGTGCAACTATTGCTAACATGTCTCCTGAATATGGTGCAACCATGGGCTTTTTTCCTGTTGATCATGTTACGTTACAATATCTAAAACTAACTGGCAGAACTGCTGACACTGTTGCTATGATAGAATCATATTTACGGGCAAATAAGATGTTCGTGGACTACAGTGAGCCCCAGTTAGAGAGAGTATACTCTGCTAGTCTTGAGCTAAAACTTGAGGATGTCGAGCCTTGCATATCCGGGCCAAAGAGGCCACATGATCGTGTTTCTCTGAAAGAAATGAAAGTTGATTGGCATGCGTGCCTTGACAATAGAGTTGGATTCAAGGGTTTTGCTGTGCCGAAGGAATCTCAAACGAAAGTCGTAGAGTTCTCATTTCATGGAACCCCCGCACAGCTTAGGCATGGAGATGTCGTAATAGCAGCTATTACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTTGGAGCTGCATTGGTTGCAAAGAAAGCTTGTGATTTAGGATTGGAGGTCAAGCCATGGATTAAGACTAGTCTTGCTCCTGGATCTGGGGTTGTAACCAAATACTTGGAAAAGAGTGGGTTGCAGAAGTATCTGAATCAGCTAGGATTTAACATTGTTGGGTATGGATGCACCACATGCATTGGAAATTCAGGAGATATTGATGACGCAGTGGCATCAGCTATTACGGAAAATGATATTGTAGCTGCTGCTGTATTGTCTGGAAATAGGAACTTTGAGGGTCGTGTACATCCTTTAACAAGGGCAAATTATCTTGCTTCTCCTCCTCTTGTGGTTGCCTATGCTCTTGCTGGCACGGTGGATATTGATTTTGAAACTGAACCTATTGGGGTGGGGAAAGACGGAAAGAAGATATTTTTCAGGGATATATGGCCATCTAATAAAGAAGTGGCAGAGGCTGTCCAATCAAATGTTCTTCCTCAGATGTTTATGGCTACATATGAAGCAATCACCCAAGGAAATCCCATGTGGAATCAGTTATCTGTACCATCTGGCAATCTTTATGCGTGGGACCCAAAGTCCACTTATATACACGAGCCACCTTACTTTAAAGATATGACCATGTCTCCTCCTGGCCCTCATGGTGTGAAGAATGCTTACTGCTTGCTCAACTTTGGAGATAGCATTACAACTGACCACATCTCACCAGCTGGTAGCATCCACAGAGAGAGCCCAGCAGCCAAATACCTTATGGAGCGCGGTGTTGATAGACGAAACTTCAACTCTTATGGTAGTCGTCGTGGTAATGATGAAATAATGGCGAGAGGCACTTTTGCAAATATTCGCCTGGTCAATAAGTTTTTGAAAGGAGAAGTTGGCCCCAAAACAATCCATCATCCCACAGGAGATAAAGTTTCAGTCTTTGATGCTGCCATGAAGTACAAAAGTGAAGGGCATGACACAATTATTCTGGCTGGTGCTGAATATGGGAGTGGAAGTTCTCGTGATTGGGCTGCTAAGGGTCCAAAGCTACTGGGGGTGAAGGCCGTCATAGCAAAAAGCTTTGAGCGCATTCACCGTAGTAATCTCGTGGGCATGGGTATCATTCCCCTATGCTTCAAGGCTGGGGAGGATGCTGAAACTCTTGGATTGACTGGTGAGGAACGCTACACCATCGACCTCCCAAGCACTGTTAGTGAAATTAAACCCGGTCAAGATATCACAGTGGTGACAAGCAATGGAAAGTCATTTGTATGCACGCTACGATTTGATACAGAGGTTGAGCTTGCGTATTTTGATCACGGAGGCATTTTGCAATATGTCATCAGGAACCTGATAAAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009987 GO:0009991 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017144 GO:0019752 GO:0030054 GO:0031667 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048027 GO:0048046 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:1901363 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

899

Amino Acids

98.09

Weight (kDa)

5.95

Isoelectric Point (pI)

31.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	65 - 568	9.6e-168	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	697 - 825	8e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011733)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35830 AT4G35830
fragaria_vesca	FvH4_1g11110
malus_domestica	MD02G1123700.v1.1 MD15G1237700.v1.1
prunus_persica	Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1 Prupe.7G174800_v2.0.a1
pyrus_communis	pycom02g09660 pycom15g21140
rosa_chinensis	RchiOBHm_Chr2g0098381
rosa_multiflora	Rmu_ssc0000194.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144020
rosa_rugosa	Rorug02G0072800
rosa_samantha	Rh2AG119800 Rh2BG122900 Rh2CG124500 Rh2DG124600
rosa_wichuraiana	Rw2G009390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	220, 259
Acc65I	GGTACC	1 cut(s)	364
AccB1I	GGYRCC	1 cut(s)	364
AccI	GTMKAC	1 cut(s)	1074
AccII	CGCG	1 cut(s)	2109
AciI	CCGC	6 cut(s)	5, 8, 57, 571, 1275, 2109
AclWI	GGATC	6 cut(s)	92, 127, 232, 499, 538, 1441
AcoI	YGGCCR	2 cut(s)	706, 1781
AcsI	RAATTY	1 cut(s)	1537
AcuI	CTGAAG	1 cut(s)	251
AfaI	GTAC	6 cut(s)	74, 122, 366, 1638, 1896, 2291
AfiI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
AflIII	ACRYGT	1 cut(s)	906
AhdI	GACNNNNNGTC	1 cut(s)	1927
AhlI	ACTAGT	1 cut(s)	1418
AjnI	CCWGG	5 cut(s)	526, 730, 1429, 1981, 2196
AjuI	GAANNNNNNNTTGG	2 cut(s)	1806, 1838
AleI	CACNNNNGTG	1 cut(s)	2576
AloI	GAACNNNNNNTCC	7 cut(s)	34, 434, 435, 466, 467, 2332, 2364
Alw21I	GWGCWC	1 cut(s)	223
Alw26I	GTCTC	2 cut(s)	915, 1980
AlwI	GGATC	6 cut(s)	92, 127, 232, 499, 538, 1441
AlwNI	CAGNNNCTG	1 cut(s)	1808
Ama87I	CYCGRG	1 cut(s)	41
AoxI	GGCC	7 cut(s)	706, 1127, 1136, 1781, 1984, 2227, 2394
ApeKI	GCWGC	7 cut(s)	1307, 1358, 1595, 1598, 2087, 2279, 2363
ApoI	RAATTY	1 cut(s)	1537
Asp700I	GAANNNNTTC	1 cut(s)	17
Asp718I	GGTACC	1 cut(s)	364
AspLEI	GCGC	2 cut(s)	2109, 2421
AspS9I	GGNCC	6 cut(s)	724, 1127, 1921, 1985, 2228, 2372
AsuC2I	CCSGG	2 cut(s)	1126, 2559
AsuHPI	GGTGA	6 cut(s)	1852, 2046, 2401, 2417, 2519, 2590
AvaI	CYCGRG	1 cut(s)	41
AvaII	GGWCC	3 cut(s)	724, 1921, 2372
BaeI	ACNNNNGTAYC	2 cut(s)	2613, 2646
BalI	TGGCCA	2 cut(s)	708, 1783
BanI	GGYRCC	1 cut(s)	364
BanII	GRGCYC	2 cut(s)	1059, 2084
BarI	GAAGNNNNNNTAC	2 cut(s)	1991, 2023
BauI	CACGAG	3 cut(s)	1943, 2352, 2438
Bbv12I	GWGCWC	1 cut(s)	223
BbvCI	CCTCAGC	1 cut(s)	815
BbvI	GCAGC	7 cut(s)	1319, 1345, 1582, 1585, 2099, 2266, 2350
BccI	CCATC	5 cut(s)	764, 1792, 1906, 2250, 2531
BceAI	ACGGC	2 cut(s)	70, 2381
BciT130I	CCWGG	5 cut(s)	528, 732, 1431, 1983, 2198
BclI	TGATCA	3 cut(s)	385, 949, 2647
BcnI	CCSGG	2 cut(s)	1126, 2559
BcoDI	GTCTC	2 cut(s)	915, 1980
BcuI	ACTAGT	1 cut(s)	1418
BfaI	CTAG	8 cut(s)	102, 255, 302, 446, 1083, 1341, 1419, 1493
BfmI	CTRYAG	2 cut(s)	76, 1048
BfuAI	ACCTGC	2 cut(s)	220, 259
BglI	GCCNNNNNGGC	1 cut(s)	1135
BglII	AGATCT	1 cut(s)	373
BisI	GCNGC	9 cut(s)	6, 58, 1308, 1359, 1596, 1599, 2088, 2280, 2364
BlsI	GCNGC	9 cut(s)	7, 59, 1309, 1360, 1597, 1600, 2089, 2281, 2365
BmcAI	AGTACT	1 cut(s)	74
Bme1390I	CCNGG	7 cut(s)	528, 732, 1126, 1431, 1983, 2198, 2559
Bme18I	GGWCC	3 cut(s)	724, 1921, 2372
BmeRI	GACNNNNNGTC	1 cut(s)	1927
BmeT110I	CYCGRG	1 cut(s)	41
BmgT120I	GGNCC	6 cut(s)	724, 1127, 1921, 1985, 2228, 2372
BmiI	GGNNCC	7 cut(s)	366, 1270, 1922, 1923, 2230, 2373, 2681
BmrFI	CCNGG	7 cut(s)	528, 732, 1126, 1431, 1983, 2198, 2559
BmrI	ACTGGG	2 cut(s)	1054, 2393
BmsI	GCATC	6 cut(s)	307, 1508, 1574, 2076, 2266, 2473
BmuI	ACTGGG	2 cut(s)	1054, 2393
BpmI	CTGGAG	1 cut(s)	161
Bpu10I	CCTNAGC	3 cut(s)	815, 1283, 2367
BpuEI	CTTGAG	3 cut(s)	317, 1109, 1121
BpuMI	CCSGG	2 cut(s)	1126, 2559
BsaJI	CCNNGG	5 cut(s)	565, 730, 930, 1406, 1864
BsaXI	ACNNNNNCTCC	9 cut(s)	32, 1284, 1314, 1960, 1990, 2247, 2277, 2332, 2362
Bsc4I	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
Bse1I	ACTGG	7 cut(s)	283, 368, 985, 1060, 1317, 2388, 2509
Bse3DI	GCAATG	1 cut(s)	2593
BseBI	CCWGG	5 cut(s)	528, 732, 1431, 1983, 2198
BseDI	CCNNGG	5 cut(s)	565, 730, 930, 1406, 1864
BseGI	GGATG	7 cut(s)	193, 1111, 1523, 1639, 2067, 2245, 2488
BseLI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
BseMI	GCAATG	1 cut(s)	2593
BseMII	CTCAG	3 cut(s)	405, 806, 1844
BseNI	ACTGG	7 cut(s)	283, 368, 985, 1060, 1317, 2388, 2509
BseRI	GAGGAG	3 cut(s)	1662, 1665, 1968
BseXI	GCAGC	7 cut(s)	1319, 1345, 1582, 1585, 2099, 2266, 2350
BseYI	CCCAGC	2 cut(s)	2083, 2474
BsgI	GTGCAG	1 cut(s)	449
Bsh1236I	CGCG	1 cut(s)	2109
BshFI	GGCC	7 cut(s)	708, 1129, 1138, 1783, 1986, 2229, 2396
BshNI	GGYRCC	1 cut(s)	364
BsiHKAI	GWGCWC	1 cut(s)	223
BsiHKCI	CYCGRG	1 cut(s)	41
BsiSI	CCGG	3 cut(s)	50, 1125, 2559
BslFI	GGGAC	2 cut(s)	634, 1934
BslI	CCNNNNNNNGG	7 cut(s)	56, 268, 533, 571, 736, 920, 1436
BsmAI	GTCTC	2 cut(s)	915, 1980
BsmFI	GGGAC	2 cut(s)	634, 1934
BsmI	GAATGC	3 cut(s)	500, 2008, 2421
BsnI	GGCC	7 cut(s)	708, 1129, 1138, 1783, 1986, 2229, 2396
BsoBI	CYCGRG	1 cut(s)	41
Bsp1286I	GDGCHC	3 cut(s)	223, 1059, 2084
Bsp1407I	TGTACA	1 cut(s)	1636
Bsp19I	CCATGG	2 cut(s)	930, 1406
BspACI	CCGC	6 cut(s)	5, 8, 57, 571, 1275, 2109
BspANI	GGCC	7 cut(s)	708, 1129, 1138, 1783, 1986, 2229, 2396
BspCNI	CTCAG	3 cut(s)	404, 807, 1843
BspFNI	CGCG	1 cut(s)	2109
BspLI	GGNNCC	7 cut(s)	366, 1270, 1922, 1923, 2230, 2373, 2681
BspMI	ACCTGC	2 cut(s)	220, 259
BspPI	GGATC	6 cut(s)	92, 127, 232, 499, 538, 1441
BspT107I	GGYRCC	1 cut(s)	364
BsrDI	GCAATG	1 cut(s)	2593
BsrGI	TGTACA	1 cut(s)	1636
BsrI	ACTGG	7 cut(s)	283, 368, 985, 1060, 1317, 2388, 2509
BssECI	CCNNGG	5 cut(s)	565, 730, 930, 1406, 1864
BssNAI	GTATAC	1 cut(s)	1075
BssSI	CACGAG	3 cut(s)	1943, 2352, 2438
BssT1I	CCWWGG	4 cut(s)	565, 930, 1406, 1864
Bst1107I	GTATAC	1 cut(s)	1075
Bst2BI	CACGAG	3 cut(s)	1943, 2352, 2438
Bst2UI	CCWGG	5 cut(s)	528, 732, 1431, 1983, 2198
BstAPI	GCANNNNNTGC	3 cut(s)	899, 1367, 1527
BstAUI	TGTACA	1 cut(s)	1636
BstDEI	CTNAG	5 cut(s)	391, 815, 1283, 1830, 2367
BstDSI	CCRYGG	2 cut(s)	930, 1406
BstENI	CCTNNNNNAGG	2 cut(s)	266, 531
BstF5I	GGATG	7 cut(s)	193, 1111, 1523, 1639, 2067, 2245, 2488
BstFNI	CGCG	1 cut(s)	2109
BstHHI	GCGC	2 cut(s)	2109, 2421
BstMAI	GTCTC	2 cut(s)	915, 1980
BstNI	CCWGG	5 cut(s)	528, 732, 1431, 1983, 2198
BstNSI	RCATGY	5 cut(s)	313, 514, 910, 1184, 1530
BstSCI	CCNGG	7 cut(s)	526, 730, 1124, 1429, 1981, 2196, 2557
BstSFI	CTRYAG	2 cut(s)	76, 1048
BstUI	CGCG	1 cut(s)	2109
BstV1I	GCAGC	7 cut(s)	1319, 1345, 1582, 1585, 2099, 2266, 2350
BstX2I	RGATCY	4 cut(s)	132, 237, 373, 1433
BstXI	CCANNNNNNTGG	1 cut(s)	1456
BstYI	RGATCY	4 cut(s)	132, 237, 373, 1433
BstZ17I	GTATAC	1 cut(s)	1075
BsuRI	GGCC	7 cut(s)	708, 1129, 1138, 1783, 1986, 2229, 2396
BtgI	CCRYGG	2 cut(s)	930, 1406
BtgZI	GCGATG	1 cut(s)	177
BtsCI	GGATG	7 cut(s)	193, 1111, 1523, 1639, 2067, 2245, 2488
BtsI	GCAGTG	1 cut(s)	1566
BtsIMutI	CAGTG	6 cut(s)	223, 996, 1057, 1566, 2538, 2580
BveI	ACCTGC	2 cut(s)	220, 259
CaiI	CAGNNNCTG	1 cut(s)	1808
CfoI	GCGC	2 cut(s)	2109, 2421
Cfr13I	GGNCC	6 cut(s)	724, 1127, 1921, 1985, 2228, 2372
CseI	GACGC	1 cut(s)	1565
Csp6I	GTAC	6 cut(s)	73, 121, 365, 1637, 1895, 2290
CspCI	CAANNNNNGTGG	4 cut(s)	625, 660, 1513, 1548
CviQI	GTAC	6 cut(s)	73, 121, 365, 1637, 1895, 2290
DdeI	CTNAG	5 cut(s)	391, 815, 1283, 1830, 2367
DraI	TTTAAA	1 cut(s)	1960
DriI	GACNNNNNGTC	1 cut(s)	1927
EaeI	YGGCCR	2 cut(s)	706, 1781
Eam1105I	GACNNNNNGTC	1 cut(s)	1927
EciI	GGCGGA	1 cut(s)	23
Eco130I	CCWWGG	4 cut(s)	565, 930, 1406, 1864
Eco24I	GRGCYC	2 cut(s)	1059, 2084
Eco32I	GATATC	1 cut(s)	2569
Eco47I	GGWCC	3 cut(s)	724, 1921, 2372
Eco57I	CTGAAG	1 cut(s)	251
Eco88I	CYCGRG	1 cut(s)	41
EcoNI	CCTNNNNNAGG	2 cut(s)	266, 531
EcoO109I	RGGNCCY	1 cut(s)	1921
EcoRII	CCWGG	5 cut(s)	526, 730, 1429, 1981, 2196
EcoRV	GATATC	1 cut(s)	2569
EcoT14I	CCWWGG	4 cut(s)	565, 930, 1406, 1864
EcoT22I	ATGCAT	2 cut(s)	502, 1532
EcoT38I	GRGCYC	2 cut(s)	1059, 2084
ErhI	CCWWGG	4 cut(s)	565, 930, 1406, 1864
FaqI	GGGAC	2 cut(s)	634, 1934
FauI	CCCGC	2 cut(s)	564, 1282
FauNDI	CATATG	1 cut(s)	1849
FbaI	TGATCA	3 cut(s)	385, 949, 2647
FblI	GTMKAC	1 cut(s)	1074
Fnu4HI	GCNGC	9 cut(s)	6, 58, 1308, 1359, 1596, 1599, 2088, 2280, 2364
FokI	GGATG	7 cut(s)	200, 1118, 1530, 1626, 2054, 2232, 2495
FriOI	GRGCYC	2 cut(s)	1059, 2084
Fsp4HI	GCNGC	9 cut(s)	6, 58, 1308, 1359, 1596, 1599, 2088, 2280, 2364
FspBI	CTAG	8 cut(s)	102, 255, 302, 446, 1083, 1341, 1419, 1493
GlaI	GCGC	2 cut(s)	2108, 2420
GluI	GCNGC	9 cut(s)	6, 58, 1308, 1359, 1596, 1599, 2088, 2280, 2364
GsaI	CCCAGC	2 cut(s)	2087, 2478
GsuI	CTGGAG	1 cut(s)	161
HaeIII	GGCC	7 cut(s)	708, 1129, 1138, 1783, 1986, 2229, 2396
HapII	CCGG	3 cut(s)	50, 1125, 2559
HgaI	GACGC	1 cut(s)	1565
HhaI	GCGC	2 cut(s)	2109, 2421
Hin6I	GCGC	2 cut(s)	2107, 2419
HinP1I	GCGC	2 cut(s)	2107, 2419
HindIII	AAGCTT	2 cut(s)	1377, 2410
HpaII	CCGG	3 cut(s)	50, 1125, 2559
HphI	GGTGA	6 cut(s)	1852, 2046, 2401, 2417, 2519, 2590
Hpy166II	GTNNAC	4 cut(s)	1045, 1075, 1637, 1932
Hpy188I	TCNGA	6 cut(s)	418, 483, 798, 1158, 1485, 1833
Hpy8I	GTNNAC	4 cut(s)	1045, 1075, 1637, 1932
Hpy99I	CGWCG	1 cut(s)	2151
HpyAV	CCTTC	6 cut(s)	146, 272, 854, 1222, 2294, 2386
HpyCH4IV	ACGT	1 cut(s)	959
HpyF3I	CTNAG	5 cut(s)	391, 815, 1283, 1830, 2367
HpySE526I	ACGT	1 cut(s)	959
HspAI	GCGC	2 cut(s)	2107, 2419
KflI	GGGWCCC	1 cut(s)	1921
KpnI	GGTACC	1 cut(s)	368
Ksp22I	TGATCA	3 cut(s)	385, 949, 2647
LmnI	GCTCC	4 cut(s)	226, 1355, 1432, 2104
Lsp1109I	GCAGC	7 cut(s)	1319, 1345, 1582, 1585, 2099, 2266, 2350
LweI	GCATC	6 cut(s)	307, 1508, 1574, 2076, 2266, 2473
MaeI	CTAG	8 cut(s)	102, 255, 302, 446, 1083, 1341, 1419, 1493
MaeII	ACGT	1 cut(s)	959
MaeIII	GTNAC	6 cut(s)	775, 802, 955, 960, 1444, 2578
MboII	GAAGA	5 cut(s)	208, 257, 1771, 1817, 2011
MflI	RGATCY	4 cut(s)	132, 237, 373, 1433
MhlI	GDGCHC	3 cut(s)	223, 1059, 2084
MlsI	TGGCCA	2 cut(s)	708, 1783
MluCI	AATT	9 cut(s)	150, 537, 553, 761, 872, 1537, 1657, 2313, 2550
MluNI	TGGCCA	2 cut(s)	708, 1783
MlyI	GAGTC	3 cut(s)	114, 152, 267
MmeI	TCCRAC	1 cut(s)	1183
Mox20I	TGGCCA	2 cut(s)	708, 1783
Mph1103I	ATGCAT	2 cut(s)	502, 1532
MroXI	GAANNNNTTC	1 cut(s)	17
MscI	TGGCCA	2 cut(s)	708, 1783
MseI	TTAA	9 cut(s)	129, 177, 354, 552, 1413, 1500, 1646, 1959, 2553
MslI	CAYNNNNRTG	5 cut(s)	638, 1146, 1293, 2576, 2602
Msp20I	TGGCCA	2 cut(s)	708, 1783
MspA1I	CMGCKG	2 cut(s)	290, 2060
MspI	CCGG	3 cut(s)	50, 1125, 2559
MspR9I	CCNGG	7 cut(s)	528, 732, 1126, 1431, 1983, 2198, 2559
Mva1269I	GAATGC	3 cut(s)	500, 2008, 2421
MvaI	CCWGG	5 cut(s)	528, 732, 1431, 1983, 2198
MvnI	CGCG	1 cut(s)	2109
NciI	CCSGG	2 cut(s)	1126, 2559
NcoI	CCATGG	2 cut(s)	930, 1406
NdeI	CATATG	1 cut(s)	1849
NlaIV	GGNNCC	7 cut(s)	366, 1270, 1922, 1923, 2230, 2373, 2681
NmuCI	GTSAC	2 cut(s)	775, 2578
NsiI	ATGCAT	2 cut(s)	502, 1532
NspI	RCATGY	5 cut(s)	313, 514, 910, 1184, 1530
OliI	CACNNNNGTG	1 cut(s)	2576
PaeI	GCATGC	2 cut(s)	313, 1184
PaeR7I	CTCGAG	1 cut(s)	41
PciI	ACATGT	1 cut(s)	906
PctI	GAATGC	3 cut(s)	500, 2008, 2421
PdmI	GAANNNNTTC	1 cut(s)	17
PfeI	GAWTC	8 cut(s)	13, 341, 626, 1013, 1206, 1232, 1486, 1882
PflFI	GACNNNGTC	1 cut(s)	1972
PfoI	TCCNGGA	2 cut(s)	526, 1429
PkrI	GCNGC	9 cut(s)	7, 59, 1309, 1360, 1597, 1600, 2089, 2281, 2365
PleI	GAGTC	3 cut(s)	113, 151, 266
PpsI	GAGTC	3 cut(s)	113, 151, 266
PpuMI	RGGWCCY	1 cut(s)	1921
PscI	ACATGT	1 cut(s)	906
Psp5II	RGGWCCY	1 cut(s)	1921
Psp6I	CCWGG	5 cut(s)	526, 730, 1429, 1981, 2196
PspFI	CCCAGC	2 cut(s)	2083, 2474
PspGI	CCWGG	5 cut(s)	526, 730, 1429, 1981, 2196
PspN4I	GGNNCC	7 cut(s)	366, 1270, 1922, 1923, 2230, 2373, 2681
PspPI	GGNCC	6 cut(s)	724, 1127, 1921, 1985, 2228, 2372
PspPPI	RGGWCCY	1 cut(s)	1921
PstNI	CAGNNNCTG	1 cut(s)	1808
PsuI	RGATCY	4 cut(s)	132, 237, 373, 1433
PsyI	GACNNNGTC	1 cut(s)	1972
PvuII	CAGCTG	2 cut(s)	290, 2060
RsaI	GTAC	6 cut(s)	74, 122, 366, 1638, 1896, 2291
RsaNI	GTAC	6 cut(s)	73, 121, 365, 1637, 1895, 2290
RseI	CAYNNNNRTG	5 cut(s)	638, 1146, 1293, 2576, 2602
SaqAI	TTAA	9 cut(s)	129, 177, 354, 552, 1413, 1500, 1646, 1959, 2553
SatI	GCNGC	9 cut(s)	6, 58, 1308, 1359, 1596, 1599, 2088, 2280, 2364
Sau96I	GGNCC	6 cut(s)	724, 1127, 1921, 1985, 2228, 2372
ScaI	AGTACT	1 cut(s)	74
SchI	GAGTC	3 cut(s)	114, 152, 267
ScrFI	CCNGG	7 cut(s)	528, 732, 1126, 1431, 1983, 2198, 2559
SduI	GDGCHC	3 cut(s)	223, 1059, 2084
SfaNI	GCATC	6 cut(s)	307, 1508, 1574, 2076, 2266, 2473
SfcI	CTRYAG	2 cut(s)	76, 1048
SfiI	GGCCNNNNNGGCC	1 cut(s)	1135
Sfr274I	CTCGAG	1 cut(s)	41
SinI	GGWCC	3 cut(s)	724, 1921, 2372
SlaI	CTCGAG	1 cut(s)	41
SmiMI	CAYNNNNRTG	5 cut(s)	638, 1146, 1293, 2576, 2602
SmlI	CTYRAG	4 cut(s)	41, 332, 1088, 1100
SmoI	CTYRAG	4 cut(s)	41, 332, 1088, 1100
SpeI	ACTAGT	1 cut(s)	1418
SphI	GCATGC	2 cut(s)	313, 1184
Sse9I	AATT	9 cut(s)	150, 537, 553, 761, 872, 1537, 1657, 2313, 2550
SsiI	CCGC	6 cut(s)	5, 8, 57, 571, 1275, 2109
SspI	AATATT	1 cut(s)	2191
SspMI	CTAG	8 cut(s)	102, 255, 302, 446, 1083, 1341, 1419, 1493
StyD4I	CCNGG	7 cut(s)	526, 730, 1124, 1429, 1981, 2196, 2557
StyI	CCWWGG	4 cut(s)	565, 930, 1406, 1864
TaiI	ACGT	1 cut(s)	962
TaqI	TCGA	5 cut(s)	42, 108, 504, 1110, 2526
TasI	AATT	9 cut(s)	150, 537, 553, 761, 872, 1537, 1657, 2313, 2550
TatI	WGTACW	3 cut(s)	72, 1636, 2289
TauI	GCSGC	2 cut(s)	8, 60
TfiI	GAWTC	8 cut(s)	13, 341, 626, 1013, 1206, 1232, 1486, 1882
Tru1I	TTAA	9 cut(s)	129, 177, 354, 552, 1413, 1500, 1646, 1959, 2553
Tru9I	TTAA	9 cut(s)	129, 177, 354, 552, 1413, 1500, 1646, 1959, 2553
TscAI	CASTG	6 cut(s)	223, 1003, 1057, 1566, 2545, 2580
TseFI	GTSAC	2 cut(s)	775, 2578
TseI	GCWGC	7 cut(s)	1307, 1358, 1595, 1598, 2087, 2279, 2363
Tsp45I	GTSAC	2 cut(s)	775, 2578
TspDTI	ATGAA	7 cut(s)	338, 533, 1181, 1253, 1866, 2175, 2300
TspGWI	ACGGA	5 cut(s)	6, 663, 1592, 1767, 2667
TspRI	CASTG	6 cut(s)	223, 1003, 1057, 1566, 2545, 2580
Tth111I	GACNNNGTC	1 cut(s)	1972
VpaK11BI	GGWCC	3 cut(s)	724, 1921, 2372
XagI	CCTNNNNNAGG	2 cut(s)	266, 531
XapI	RAATTY	1 cut(s)	1537
XceI	RCATGY	5 cut(s)	313, 514, 910, 1184, 1530
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2098
XhoI	CTCGAG	1 cut(s)	41
XmiI	GTMKAC	1 cut(s)	1074
XmnI	GAANNNNTTC	1 cut(s)	17
XspI	CTAG	8 cut(s)	102, 255, 302, 446, 1083, 1341, 1419, 1493
ZrmI	AGTACT	1 cut(s)	74
Zsp2I	ATGCAT	2 cut(s)	502, 1532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.