Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012828

Physical Location & Seq

Genomic Coordinates Forward (+)

383153 .. 385910

Gene Structure 2758 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G02010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGGATGGTTCGTCGGAAAATTTGCCAGAAGTGGAAGAGAAAGGTAGAGAAAGCAGTTGCTGCAGCTCGGAAACAACCAGACAAGAGGAGGAAGAACAAAGCCCTAGCTGTACGGAGGATTTCACGGCTTCTCCGGTTTCATCTCGTTGGTCTGTGAAGAACATCGATGGAGAGAAGAAGAAGATTCGTTCGGATTCAAGAGTTTCAGAGGTTGAGATGATGAAGGAAAGATTTTCGAAACTGCTGCTTGGAGAAGATATGTCAGGTTCTGGAAATGGTGTCTGTACAGCTTTAGCTATCTCAAACGCAATCACTAATCTTTGTGCTACCTTGTTTGGACAACTATGGAGGTTAGAACCACTGCCTACAGAGAAGAAAGAGATGTGGAGAAGAGAAATGGAATGGCTTCTCTGTGTCAGTGATCACATTGTAGAGATGACTCCTACTTGGCAAACATTTCCTGATGGAACCAAACTTGAGATAATGACTTGCAGACCAAGATCAGATCTTTATGTTAACCTCCCAGCTCTCAGGAAACTAGATAACATGCTTCTTGAGATATTAGATAGTTTTGAGGAGACAGAGTTTTGGTATGTTGACCAAGGAATCATGGCACACGAATCAGCAGCGGACGGATCATCTTCTTTCCGAAAATCTTTTCAGAGACAAGAAGACAAATGGTGGCTTCCTGTTCCACGGGTTTCACCAGGAGGTTTACAAGAAAACTCAAGAAAACAGTTGCAGCATAAACGCGACTGTACTAATCAGATATTAAAAGCTGCTATGGCCATCAACAGTATCACACTCGCTGACATGGAAATCCCTGAATCATACCTTGAATCTCTTCCGCGGAAAGGGAGATCTTGCCTTGGTGACTTAATCTACAGATACATTTCATCGGATCAATTTTCACCTGAATGTCTTCTTGATTGCCTTGACTTATCCTCTGAACATCAAGCCATAGAGATAGCTAACCGAGTCGAGTCATCGATCTATCTATGGCACAAAAGAACTAATTCCAAACCAGCTACTAATACCAAAACGTCGTGGGAAATGGTGAAAGAACTAATGGTTGATGCAGATAAGCTAGAGCTAATGGCGGATCGAGCTGAAAGTTTATTGCTTTCTTTAAAACAACGATTCCCAGGTCTTCCTCAAACCGCTCTAGACATGAGCAAAATCCAATACAACAAGGACATCGGGAAATCGATTCTGGAAAGCTATTCAAGAGTTCTAGAGAGCTTAGCATTCAACATTGTTGCACGCATCGATGATTTGCTCTTTGTTGATGATCTTACAAGACATTCATCGGATCAGATTCCTACAACATTAGGCAACAACGGGAATGACGCTCCTAAGAGCATTGCAGTGCCGGTTTCAAACTACACAACTCCGAGCTACTCGCCTTCAAAACAGGAGCTTCGATCATCAATTACAGTTCCACCATCTCCCTCCCGGTTCAAGATTCCTCACAGTAGCAGTGTTAAAAGGGTTTTGACAGCTTATGTAACAAAGAACGAACCAAGACTGAAGAATCTTCCTTTAGAAAGATCGAGCCGTTCTTCGTCGAGTGAGAGATTGTCTCTGGAGAAATGCATGAAGGAGTCGTTGAATGTATCGAACCTTGATCCTGGGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0031267 GO:0032092 GO:0032501 GO:0032502 GO:0032879 GO:0032989 GO:0040007 GO:0040008 GO:0043393 GO:0043900 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048518 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051020 GO:0051049 GO:0051098 GO:0051099 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0065008 GO:0065009 GO:0071840 GO:0071944 GO:0080092 GO:0098590 GO:0098772 GO:1904424 GO:1904426 GO:1904475 GO:1904477 GO:1905097 GO:1905099 GO:2000012 GO:2000241 GO:2001106 GO:2001108

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PRONE

Protein Analysis

546

Amino Acids

61.75

Weight (kDa)

5.39

Isoelectric Point (pI)

66.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	69 - 434	3.1e-184	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02010
fragaria_vesca	FvH4_6g12700
malus_domestica	MD04G1139300.v1.1 MD12G1155100.v1.1
prunus_persica	Prupe.6G264800_v2.0.a1
pyrus_communis	pycom04g12750 pycom12g14720
rosa_chinensis	RchiOBHm_Chr3g0464551
rosa_laevigata	RLG00000024683
rosa_multiflora	Rmu_sc0002031.1_g000015 Rmu_sc0014550.1_g000001 Rmu_sc0042806.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415540
rosa_rugosa	Rorug03G0073100
rosa_samantha	Rh3BG135300 Rh3CG138000 Rh3DG136900
rosa_wichuraiana	Rw3G011080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1163
AccII	CGCG	2 cut(s)	753, 850
AciI	CCGC	5 cut(s)	629, 848, 850, 1100, 1161
AclWI	GGATC	5 cut(s)	643, 909, 1110, 1320, 1622
AcoI	YGGCCR	1 cut(s)	786
AcsI	RAATTY	1 cut(s)	19
AcuI	CTGAAG	1 cut(s)	1550
AfaI	GTAC	3 cut(s)	112, 286, 760
AfiI	CCNNNNNNNGG	1 cut(s)	854
AgsI	TTSAA	8 cut(s)	198, 839, 1227, 1252, 1380, 1410, 1462, 1612
AjnI	CCWGG	3 cut(s)	706, 1144, 1630
Alw26I	GTCTC	3 cut(s)	572, 658, 1587
AlwI	GGATC	5 cut(s)	643, 909, 1110, 1320, 1622
AlwNI	CAGNNNCTG	2 cut(s)	60, 269
AoxI	GGCC	1 cut(s)	786
ApeKI	GCWGC	6 cut(s)	60, 63, 244, 626, 742, 779
ApoI	RAATTY	1 cut(s)	19
Asp700I	GAANNNNTTC	4 cut(s)	405, 843, 921, 1015
AsuC2I	CCSGG	1 cut(s)	1456
AsuHPI	GGTGA	4 cut(s)	696, 884, 903, 1069
AsuII	TTCGAA	1 cut(s)	236
BalI	TGGCCA	1 cut(s)	788
BbsI	GAAGAC	3 cut(s)	678, 914, 1142
BbvI	GCAGC	6 cut(s)	47, 75, 231, 638, 754, 766
BccI	CCATC	4 cut(s)	161, 458, 797, 1453
BceAI	ACGGC	2 cut(s)	141, 1542
BcgI	CGANNNNNNTGC	2 cut(s)	226, 260
BciT130I	CCWGG	3 cut(s)	708, 1146, 1632
BclI	TGATCA	1 cut(s)	421
BcnI	CCSGG	1 cut(s)	1456
BcoDI	GTCTC	3 cut(s)	572, 658, 1587
BfaI	CTAG	5 cut(s)	105, 539, 1088, 1166, 1235
BfmI	CTRYAG	3 cut(s)	61, 366, 883
BglII	AGATCT	2 cut(s)	505, 860
BisI	GCNGC	6 cut(s)	61, 64, 245, 627, 743, 780
BlpI	GCTNAGC	1 cut(s)	1243
BlsI	GCNGC	6 cut(s)	62, 65, 246, 628, 744, 781
Bme1390I	CCNGG	4 cut(s)	708, 1146, 1456, 1632
BmiI	GGNNCC	1 cut(s)	469
BmrFI	CCNGG	4 cut(s)	708, 1146, 1456, 1632
BmsI	GCATC	2 cut(s)	1066, 1275
BpiI	GAAGAC	3 cut(s)	678, 914, 1142
BplI	GAGNNNNNCTC	2 cut(s)	1567, 1599
BpmI	CTGGAG	1 cut(s)	1607
Bpu1102I	GCTNAGC	1 cut(s)	1243
Bpu14I	TTCGAA	1 cut(s)	236
BpuEI	CTTGAG	3 cut(s)	497, 575, 712
BpuMI	CCSGG	1 cut(s)	1456
Bsa29I	ATCGAT	4 cut(s)	165, 989, 1208, 1269
BsaJI	CCNNGG	6 cut(s)	601, 695, 848, 868, 1144, 1631
BsaWI	WCCGGW	1 cut(s)	133
BsaXI	ACNNNNNCTCC	4 cut(s)	115, 145, 569, 599
Bsc4I	CCNNNNNNNGG	1 cut(s)	854
Bse118I	RCCGGY	1 cut(s)	1372
Bse3DI	GCAATG	1 cut(s)	1362
BseBI	CCWGG	3 cut(s)	708, 1146, 1632
BseCI	ATCGAT	4 cut(s)	165, 989, 1208, 1269
BseDI	CCNNGG	6 cut(s)	601, 695, 848, 868, 1144, 1631
BseGI	GGATG	1 cut(s)	10
BseLI	CCNNNNNNNGG	1 cut(s)	854
BseMI	GCAATG	1 cut(s)	1362
BseMII	CTCAG	1 cut(s)	544
BseRI	GAGGAG	2 cut(s)	101, 590
BseXI	GCAGC	6 cut(s)	47, 75, 231, 638, 754, 766
BseYI	CCCAGC	1 cut(s)	523
Bsh1236I	CGCG	2 cut(s)	753, 850
BshFI	GGCC	1 cut(s)	788
BshVI	ATCGAT	4 cut(s)	165, 989, 1208, 1269
BsiSI	CCGG	3 cut(s)	134, 1373, 1456
BslI	CCNNNNNNNGG	1 cut(s)	854
BsmAI	GTCTC	3 cut(s)	572, 658, 1587
BsmI	GAATGC	1 cut(s)	1247
BsnI	GGCC	1 cut(s)	788
Bsp119I	TTCGAA	1 cut(s)	236
Bsp1407I	TGTACA	1 cut(s)	284
Bsp1720I	GCTNAGC	1 cut(s)	1243
BspACI	CCGC	5 cut(s)	629, 848, 850, 1100, 1161
BspANI	GGCC	1 cut(s)	788
BspCNI	CTCAG	1 cut(s)	543
BspDI	ATCGAT	4 cut(s)	165, 989, 1208, 1269
BspFNI	CGCG	2 cut(s)	753, 850
BspLI	GGNNCC	1 cut(s)	469
BspMAI	CTGCAG	1 cut(s)	65
BspPI	GGATC	5 cut(s)	643, 909, 1110, 1320, 1622
BspT104I	TTCGAA	1 cut(s)	236
BsrBI	CCGCTC	1 cut(s)	1163
BsrDI	GCAATG	1 cut(s)	1362
BsrFI	RCCGGY	1 cut(s)	1372
BsrGI	TGTACA	1 cut(s)	284
BssAI	RCCGGY	1 cut(s)	1372
BssECI	CCNNGG	6 cut(s)	601, 695, 848, 868, 1144, 1631
BssT1I	CCWWGG	2 cut(s)	601, 868
Bst2UI	CCWGG	3 cut(s)	708, 1146, 1632
Bst4CI	ACNGT	5 cut(s)	738, 758, 797, 1438, 1475
Bst6I	CTCTTC	3 cut(s)	30, 385, 849
BstAPI	GCANNNNNTGC	1 cut(s)	60
BstAUI	TGTACA	1 cut(s)	284
BstBI	TTCGAA	1 cut(s)	236
BstC8I	GCNNGC	1 cut(s)	1264
BstDEI	CTNAG	3 cut(s)	530, 1243, 1356
BstDSI	CCRYGG	2 cut(s)	695, 848
BstF5I	GGATG	1 cut(s)	10
BstFNI	CGCG	2 cut(s)	753, 850
BstMAI	GTCTC	3 cut(s)	572, 658, 1587
BstMWI	GCNNNNNNNGC	3 cut(s)	60, 785, 1106
BstNI	CCWGG	3 cut(s)	708, 1146, 1632
BstNSI	RCATGY	1 cut(s)	550
BstSCI	CCNGG	4 cut(s)	706, 1144, 1454, 1630
BstSFI	CTRYAG	3 cut(s)	61, 366, 883
BstUI	CGCG	2 cut(s)	753, 850
BstV1I	GCAGC	6 cut(s)	47, 75, 231, 638, 754, 766
BstV2I	GAAGAC	3 cut(s)	678, 914, 1142
BstX2I	RGATCY	2 cut(s)	505, 860
BstYI	RGATCY	2 cut(s)	505, 860
Bsu15I	ATCGAT	4 cut(s)	165, 989, 1208, 1269
BsuRI	GGCC	1 cut(s)	788
BsuTUI	ATCGAT	4 cut(s)	165, 989, 1208, 1269
BtgI	CCRYGG	2 cut(s)	695, 848
BtsCI	GGATG	1 cut(s)	10
BtsI	GCAGTG	3 cut(s)	359, 1374, 1486
BtsIMutI	CAGTG	4 cut(s)	359, 424, 1374, 1486
Cac8I	GCNNGC	1 cut(s)	1264
CaiI	CAGNNNCTG	2 cut(s)	60, 269
Cfr10I	RCCGGY	1 cut(s)	1372
Cfr42I	CCGCGG	1 cut(s)	851
ClaI	ATCGAT	4 cut(s)	165, 989, 1208, 1269
CseI	GACGC	1 cut(s)	1358
Csp6I	GTAC	3 cut(s)	111, 285, 759
CviAII	CATG	5 cut(s)	547, 610, 814, 1171, 1597
CviQI	GTAC	3 cut(s)	111, 285, 759
DdeI	CTNAG	3 cut(s)	530, 1243, 1356
DraI	TTTAAA	1 cut(s)	1131
EaeI	YGGCCR	1 cut(s)	786
Eam1104I	CTCTTC	3 cut(s)	30, 385, 849
EarI	CTCTTC	3 cut(s)	30, 385, 849
EciI	GGCGGA	1 cut(s)	1115
Eco130I	CCWWGG	2 cut(s)	601, 868
Eco57I	CTGAAG	1 cut(s)	1550
EcoRII	CCWGG	3 cut(s)	706, 1144, 1630
EcoT14I	CCWWGG	2 cut(s)	601, 868
EcoT22I	ATGCAT	1 cut(s)	1598
ErhI	CCWWGG	2 cut(s)	601, 868
FaeI	CATG	5 cut(s)	550, 613, 817, 1174, 1600
FatI	CATG	5 cut(s)	546, 609, 813, 1170, 1596
FbaI	TGATCA	1 cut(s)	421
Fnu4HI	GCNGC	6 cut(s)	61, 64, 245, 627, 743, 780
FokI	GGATG	1 cut(s)	17
Fsp4HI	GCNGC	6 cut(s)	61, 64, 245, 627, 743, 780
FspBI	CTAG	5 cut(s)	105, 539, 1088, 1166, 1235
GluI	GCNGC	6 cut(s)	61, 64, 245, 627, 743, 780
GsaI	CCCAGC	1 cut(s)	527
GsuI	CTGGAG	1 cut(s)	1607
HaeIII	GGCC	1 cut(s)	788
HapII	CCGG	3 cut(s)	134, 1373, 1456
HgaI	GACGC	1 cut(s)	1358
Hin1II	CATG	5 cut(s)	550, 613, 817, 1174, 1600
HincII	GTYRAC	2 cut(s)	517, 598
HindII	GTYRAC	2 cut(s)	517, 598
HpaI	GTTAAC	1 cut(s)	517
HpaII	CCGG	3 cut(s)	134, 1373, 1456
HphI	GGTGA	4 cut(s)	696, 884, 903, 1069
Hpy166II	GTNNAC	3 cut(s)	517, 598, 716
Hpy8I	GTNNAC	3 cut(s)	517, 598, 716
Hpy99I	CGWCG	3 cut(s)	16, 1048, 1570
HpyAV	CCTTC	3 cut(s)	217, 1416, 1594
HpyCH4III	ACNGT	5 cut(s)	738, 758, 797, 1438, 1475
HpyCH4IV	ACGT	1 cut(s)	1043
HpyCH4V	TGCA	7 cut(s)	63, 492, 742, 1079, 1262, 1367, 1596
HpyF10VI	GCNNNNNNNGC	3 cut(s)	60, 785, 1106
HpyF3I	CTNAG	3 cut(s)	530, 1243, 1356
HpySE526I	ACGT	1 cut(s)	1043
Hsp92II	CATG	5 cut(s)	550, 613, 817, 1174, 1600
Ksp22I	TGATCA	1 cut(s)	421
KspAI	GTTAAC	1 cut(s)	517
KspI	CCGCGG	1 cut(s)	851
LmnI	GCTCC	2 cut(s)	1357, 1417
Lsp1109I	GCAGC	6 cut(s)	47, 75, 231, 638, 754, 766
LweI	GCATC	2 cut(s)	1066, 1275
MaeI	CTAG	5 cut(s)	105, 539, 1088, 1166, 1235
MaeII	ACGT	1 cut(s)	1043
MaeIII	GTNAC	2 cut(s)	872, 1507
MbiI	CCGCTC	1 cut(s)	1163
MflI	RGATCY	2 cut(s)	505, 860
MlsI	TGGCCA	1 cut(s)	788
MluCI	AATT	4 cut(s)	19, 905, 1015, 1431
MluNI	TGGCCA	1 cut(s)	788
MlyI	GAGTC	4 cut(s)	433, 987, 992, 1613
Mox20I	TGGCCA	1 cut(s)	788
Mph1103I	ATGCAT	1 cut(s)	1598
MroXI	GAANNNNTTC	4 cut(s)	405, 843, 921, 1015
MscI	TGGCCA	1 cut(s)	788
MseI	TTAA	5 cut(s)	516, 773, 878, 1130, 1485
MslI	CAYNNNNRTG	2 cut(s)	916, 1367
Msp20I	TGGCCA	1 cut(s)	788
MspA1I	CMGCKG	2 cut(s)	629, 850
MspI	CCGG	3 cut(s)	134, 1373, 1456
MspR9I	CCNGG	4 cut(s)	708, 1146, 1456, 1632
Mva1269I	GAATGC	1 cut(s)	1247
MvaI	CCWGG	3 cut(s)	708, 1146, 1632
MvnI	CGCG	2 cut(s)	753, 850
MwoI	GCNNNNNNNGC	3 cut(s)	60, 785, 1106
NciI	CCSGG	1 cut(s)	1456
NlaIII	CATG	5 cut(s)	550, 613, 817, 1174, 1600
NlaIV	GGNNCC	1 cut(s)	469
NmuCI	GTSAC	1 cut(s)	872
NsiI	ATGCAT	1 cut(s)	1598
NspI	RCATGY	1 cut(s)	550
NspV	TTCGAA	1 cut(s)	236
PctI	GAATGC	1 cut(s)	1247
PdmI	GAANNNNTTC	4 cut(s)	405, 843, 921, 1015
PkrI	GCNGC	6 cut(s)	62, 65, 246, 628, 744, 781
PleI	GAGTC	4 cut(s)	433, 986, 991, 1612
PpsI	GAGTC	4 cut(s)	433, 986, 991, 1612
Psp6I	CCWGG	3 cut(s)	706, 1144, 1630
PspFI	CCCAGC	1 cut(s)	523
PspGI	CCWGG	3 cut(s)	706, 1144, 1630
PspN4I	GGNNCC	1 cut(s)	469
PstI	CTGCAG	1 cut(s)	65
PstNI	CAGNNNCTG	2 cut(s)	60, 269
PsuI	RGATCY	2 cut(s)	505, 860
RsaI	GTAC	3 cut(s)	112, 286, 760
RsaNI	GTAC	3 cut(s)	111, 285, 759
RseI	CAYNNNNRTG	2 cut(s)	916, 1367
SacII	CCGCGG	1 cut(s)	851
SaqAI	TTAA	5 cut(s)	516, 773, 878, 1130, 1485
SatI	GCNGC	6 cut(s)	61, 64, 245, 627, 743, 780
SchI	GAGTC	4 cut(s)	433, 987, 992, 1613
ScrFI	CCNGG	4 cut(s)	708, 1146, 1456, 1632
SfaNI	GCATC	2 cut(s)	1066, 1275
SfcI	CTRYAG	3 cut(s)	61, 366, 883
Sfr303I	CCGCGG	1 cut(s)	851
SfuI	TTCGAA	1 cut(s)	236
SgrBI	CCGCGG	1 cut(s)	851
SmiMI	CAYNNNNRTG	2 cut(s)	916, 1367
SmlI	CTYRAG	3 cut(s)	476, 554, 727
SmoI	CTYRAG	3 cut(s)	476, 554, 727
Sse9I	AATT	4 cut(s)	19, 905, 1015, 1431
SsiI	CCGC	5 cut(s)	629, 848, 850, 1100, 1161
SspMI	CTAG	5 cut(s)	105, 539, 1088, 1166, 1235
StyD4I	CCNGG	4 cut(s)	706, 1144, 1454, 1630
StyI	CCWWGG	2 cut(s)	601, 868
TaaI	ACNGT	5 cut(s)	738, 758, 797, 1438, 1475
TaiI	ACGT	1 cut(s)	1046
TasI	AATT	4 cut(s)	19, 905, 1015, 1431
TatI	WGTACW	2 cut(s)	284, 758
Tru1I	TTAA	5 cut(s)	516, 773, 878, 1130, 1485
Tru9I	TTAA	5 cut(s)	516, 773, 878, 1130, 1485
TscAI	CASTG	4 cut(s)	366, 424, 1374, 1486
TseFI	GTSAC	1 cut(s)	872
TseI	GCWGC	6 cut(s)	60, 63, 244, 626, 742, 779
Tsp45I	GTSAC	1 cut(s)	872
TspDTI	ATGAA	5 cut(s)	129, 236, 885, 1296, 1613
TspGWI	ACGGA	2 cut(s)	128, 648
TspRI	CASTG	4 cut(s)	366, 424, 1374, 1486
XapI	RAATTY	1 cut(s)	19
XbaI	TCTAGA	2 cut(s)	1165, 1234
XceI	RCATGY	1 cut(s)	550
XmnI	GAANNNNTTC	4 cut(s)	405, 843, 921, 1015
XspI	CTAG	5 cut(s)	105, 539, 1088, 1166, 1235
Zsp2I	ATGCAT	1 cut(s)	1598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.