Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012828

Physical Location & Seq

Genomic Coordinates Reverse (-)

23558325 .. 23561967

Gene Structure 3643 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1155100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGGACAGAGCTTTCACTCACCAAGAGGAAAGAGAAGGAAGAACCCACCTACAAGATCACCACCTCAAGCTCTGTCTGCCATTACTGAGCTTCAGAAGCCTCAGCTCCCACAACCCATTTTCTCTTTTCGGTTTCTGGGCGTCGAAGTATCTCCGGAACTTGCATTGCAGAGCTCGAATCTCCAACTCCTGCTGCCTGGGAAGGCTTCGGTACGATGGTATGATGGTGAACAACTCTGCTTTCTGTGATTCGCCGGGTTTTGGTGACGAAGGAGGAGCTTCAATGGAGGGTTTGGTGGAGAAAGATGACGTTATTGGGGAGAAAAGTGACGGATTTTTTGGTGAAGCTTTGGATAAAGTTGGGAGCTTTGAGGATTTGATTGAGGAGAAGGGTCGTGAGAGCAGTTCAAGTTCGGATTGTCTGACGTCGGAGACGACGGGGCACGAGGAGGAGCAGAGCCACAGCAGCTCGGAGGAGTCATCTTGGCCGCCGTCGTTGGGTTGGCCTGTTCAGAAAGGCGAGGCGCCGGATTGCACCAGTGAGAATGGTGATGAAGCGGCGGAGAAAACCCATTTGGATGATAGGAAACTAGAGAAACAAGGGTCGACCATATCAGAGACTGAGTTGATGAAGGAAAGGTTTTCAAAATTGCTGCTTGGAGAAGACATGTCAGGTTGTGGAAATGGAGTTTGCACAGCATTGGCGATATCAAATGCCATTACTAATCTTTGTGCTACCCTTTTCGGGCAACTTTGGAGACTAGAACCTCTACCAAAAGTGAAGAAATCAATGTGGCAAAGAGAGATGGAATGGCTTCTTTGTGTGAGTGATCACATTGTTGAGTTGATGCCTTCTTGGCAGACTTTTCCTGATGGAAGCAAATTGGAGGTCATGACTTGCAGACCGCGCTCAGATCTTTACGTTAATCTCCCTGCCCTTCGTAAATTGGATAACATGCTTCTTGAAATCTTAGATGGCTTTGACAATACAGAGTTCTGGTATGTTGATCAAGGGATTCTAGCCCCAGATGCGGATGGGTCAACCTCTTTCCGAAAAGCGTTACAGCGCCAAGAGGAAAAATGGTGGCTACCTGTGCCCCGAGTTCCTTCCGGTGGACTCCATAAAAATTCAAGAAAGCAATTGCAGCACAAACGCGATTGCACGAGTCAAATATTAAAAGCTGCCATGGCAATCAATAGTATTACTTTAGCAGATATGGAAGTCCCTGAATCATATCTGGAATCTCTTCCAAAGAATGGAAAAGCAAGCTTAGGGGATGTTATTTATCGATACATTACGTCAGATCATTTTTCCGCAGAATGCCTGCTTGATTTCCTTGATTTGTCTTCTGAACATCAAGCCATAGAGATTGCGAATAGAGTGGAGGCCTCGATCTATCTGTGGCATAAAAAAGCAAACCCAAAACCTACGAGTATTGTGAACAGATTTGGTTCGAAGTCATCATGGGAAATGGTCAAGGAGCTGATGATTGATGCAGAGAAAAAGGAGTTGCTTCCAGAAAGAGCAGAAAGTTTGTTAATGTGTTTGAAGCAACGGTTCCCTGGTCTTCCACAGACAGCCCTCGATATGAGCAAGATTCAGTACAATAAGGATGTCGGGAAATCCATCTTGGAGAGCTATTCAAGGGTTTTGGAGAGCCTGGCATTTAACATTGTGGCACGCATTGATGACCTGCTTTATGTAGATGACTTGATGCGGCATTCTGATCAGTTCCCATCAATCTCGAACGTGAGTCTGATTTCACACAAGAGCAATCCTACTCCATACTCGGTGCCCATCTCAAGCACACCATACAAAACAGCTTTCACCACACCAAGCTTTTCACCCGCACAGCTAGTTAGCCCTCTGAGGGGAGACAGATCACCATTCATCACCATCGGCAAGATCCCTCAGCATGGGTTGGGTGTAAAAAAAGTTCTGACTGATTATCTTAACATCGATGCAACAGGAAAGGACTACAGTGATCCAATTGAGAAACCAGATTCAGTATCGAACACAATGCAAGAAACGATAGGGTCTCACGTGCGAATGGATTCTTTTGAGCGTACAAAAGCATCAAGAAGCCTGGAATCACAGGACTCATTCACGCAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0031267 GO:0032092 GO:0032501 GO:0032502 GO:0032879 GO:0032989 GO:0040007 GO:0040008 GO:0043393 GO:0043900 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048518 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051020 GO:0051049 GO:0051098 GO:0051099 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0065008 GO:0065009 GO:0071840 GO:0071944 GO:0080092 GO:0098590 GO:0098772 GO:1904424 GO:1904426 GO:1904475 GO:1904477 GO:1905097 GO:1905099 GO:2000012 GO:2000241 GO:2001106 GO:2001108

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PRONE

Protein Analysis

706

Amino Acids

79.16

Weight (kDa)

5.35

Isoelectric Point (pI)

50.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	205 - 573	1.7e-183	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02010
fragaria_vesca	FvH4_6g12700
malus_domestica	MD04G1139300.v1.1 MD12G1155100.v1.1
prunus_persica	Prupe.6G264800_v2.0.a1
pyrus_communis	pycom04g12750 pycom12g14720
rosa_chinensis	RchiOBHm_Chr3g0464551
rosa_laevigata	RLG00000024683
rosa_multiflora	Rmu_sc0002031.1_g000015 Rmu_sc0014550.1_g000001 Rmu_sc0042806.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415540
rosa_rugosa	Rorug03G0073100
rosa_samantha	Rh3BG135300 Rh3CG138000 Rh3DG136900
rosa_wichuraiana	Rw3G011080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	428
Acc36I	ACCTGC	1 cut(s)	1701
AccB1I	GGYRCC	2 cut(s)	523, 1792
AccB7I	CCANNNNNTGG	1 cut(s)	1256
AccI	GTMKAC	1 cut(s)	605
AccII	CGCG	2 cut(s)	907, 1155
AccIII	TCCGGA	1 cut(s)	153
AciI	CCGC	8 cut(s)	488, 557, 560, 905, 1031, 1314, 1717, 1848
AclWI	GGATC	2 cut(s)	1900, 1979
AcoI	YGGCCR	1 cut(s)	485
AcsI	RAATTY	1 cut(s)	1126
AcuI	CTGAAG	1 cut(s)	76
AcvI	CACGTG	1 cut(s)	2044
AcyI	GRCGYC	3 cut(s)	140, 425, 524
AfaI	GTAC	3 cut(s)	212, 1604, 2068
AfiI	CCNNNNNNNGG	9 cut(s)	260, 497, 744, 1030, 1112, 1256, 1870, 1871, 1916
AflIII	ACRYGT	1 cut(s)	666
AgsI	TTSAA	7 cut(s)	282, 408, 645, 965, 1131, 1549, 1644
AjnI	CCWGG	4 cut(s)	195, 1561, 1659, 2085
AjuI	GAANNNNNNNTTGG	4 cut(s)	557, 589, 1613, 1645
Alw21I	GWGCWC	1 cut(s)	175
Alw26I	GTCTC	5 cut(s)	425, 611, 751, 1869, 2043
AlwI	GGATC	2 cut(s)	1900, 1979
AlwNI	CAGNNNCTG	1 cut(s)	620
Ama87I	CYCGRG	1 cut(s)	1098
Aor13HI	TCCGGA	1 cut(s)	153
AoxI	GGCC	3 cut(s)	485, 503, 1386
ApeKI	GCWGC	5 cut(s)	192, 465, 652, 1144, 1181
ApoI	RAATTY	1 cut(s)	1126
ArsI	GACNNNNNNTTYG	4 cut(s)	320, 352, 873, 905
Asp700I	GAANNNNTTC	3 cut(s)	813, 1245, 2053
AspLEI	GCGC	3 cut(s)	526, 909, 1068
AsuC2I	CCSGG	1 cut(s)	255
AsuII	TTCGAA	1 cut(s)	1454
AvaI	CYCGRG	1 cut(s)	1098
BaeGI	GKGCMC	3 cut(s)	444, 1098, 1797
BaeI	ACNNNNGTAYC	2 cut(s)	202, 235
BanI	GGYRCC	2 cut(s)	523, 1792
BanII	GRGCYC	1 cut(s)	175
BauI	CACGAG	2 cut(s)	443, 1162
BbrPI	CACGTG	1 cut(s)	2044
BbsI	GAAGAC	3 cut(s)	669, 1338, 1559
Bbv12I	GWGCWC	1 cut(s)	175
BbvCI	CCTCAGC	2 cut(s)	101, 1911
BbvI	GCAGC	5 cut(s)	179, 477, 639, 1156, 1168
BceAI	ACGGC	1 cut(s)	475
BcgI	CGANNNNNNTGC	2 cut(s)	2002, 2036
BciT130I	CCWGG	4 cut(s)	197, 1563, 1661, 2087
BclI	TGATCA	3 cut(s)	829, 1006, 1726
BcnI	CCSGG	1 cut(s)	255
BcoDI	GTCTC	5 cut(s)	425, 611, 751, 1869, 2043
BfaI	CTAG	4 cut(s)	590, 761, 1019, 1856
BfmI	CTRYAG	1 cut(s)	1978
BfoI	RGCGCY	2 cut(s)	527, 1069
BfuAI	ACCTGC	1 cut(s)	1701
BglI	GCCNNNNNGGC	1 cut(s)	856
BglII	AGATCT	1 cut(s)	913
BisI	GCNGC	8 cut(s)	193, 466, 488, 558, 653, 1145, 1182, 1718
BlsI	GCNGC	8 cut(s)	194, 467, 489, 559, 654, 1146, 1183, 1719
Bme1390I	CCNGG	5 cut(s)	197, 255, 1563, 1661, 2087
BmeT110I	CYCGRG	1 cut(s)	1098
BmiI	GGNNCC	3 cut(s)	525, 1559, 1794
BmrFI	CCNGG	5 cut(s)	197, 255, 1563, 1661, 2087
BmsI	GCATC	6 cut(s)	837, 1018, 1483, 1704, 1951, 2084
BpiI	GAAGAC	3 cut(s)	669, 1338, 1559
Bpu10I	CCTNAGC	3 cut(s)	101, 1270, 1911
Bpu14I	TTCGAA	1 cut(s)	1454
BpuEI	CTTGAG	2 cut(s)	50, 1786
BpuMI	CCSGG	1 cut(s)	255
Bsa29I	ATCGAT	2 cut(s)	1288, 1959
BsaAI	YACGTR	1 cut(s)	2044
BsaHI	GRCGYC	3 cut(s)	140, 425, 524
BsaI	GGTCTC	1 cut(s)	2043
BsaJI	CCNNGG	3 cut(s)	196, 1185, 1561
BsaWI	WCCGGW	2 cut(s)	153, 1109
Bsc4I	CCNNNNNNNGG	9 cut(s)	260, 497, 744, 1030, 1112, 1256, 1870, 1871, 1916
Bse1I	ACTGG	1 cut(s)	537
Bse3DI	GCAATG	1 cut(s)	163
BseAI	TCCGGA	1 cut(s)	153
BseBI	CCWGG	4 cut(s)	197, 1563, 1661, 2087
BseCI	ATCGAT	2 cut(s)	1288, 1959
BseDI	CCNNGG	3 cut(s)	196, 1185, 1561
BseGI	GGATG	4 cut(s)	583, 1039, 1282, 1618
BseLI	CCNNNNNNNGG	9 cut(s)	260, 497, 744, 1030, 1112, 1256, 1870, 1871, 1916
BseMI	GCAATG	1 cut(s)	163
BseMII	CTCAG	6 cut(s)	77, 115, 612, 924, 1859, 1925
BseNI	ACTGG	1 cut(s)	537
BseRI	GAGGAG	5 cut(s)	288, 398, 461, 464, 488
BseSI	GKGCMC	3 cut(s)	444, 1098, 1797
BseXI	GCAGC	5 cut(s)	179, 477, 639, 1156, 1168
Bsh1236I	CGCG	2 cut(s)	907, 1155
BshFI	GGCC	3 cut(s)	487, 505, 1388
BshNI	GGYRCC	2 cut(s)	523, 1792
BshVI	ATCGAT	2 cut(s)	1288, 1959
BsiHKAI	GWGCWC	1 cut(s)	175
BsiHKCI	CYCGRG	1 cut(s)	1098
BsiSI	CCGG	4 cut(s)	154, 254, 527, 1110
BslFI	GGGAC	1 cut(s)	1208
BslI	CCNNNNNNNGG	9 cut(s)	260, 497, 744, 1030, 1112, 1256, 1870, 1871, 1916
BsmAI	GTCTC	5 cut(s)	425, 611, 751, 1869, 2043
BsmBI	CGTCTC	1 cut(s)	425
BsmFI	GGGAC	1 cut(s)	1208
BsmI	GAATGC	2 cut(s)	1325, 1720
BsnI	GGCC	3 cut(s)	487, 505, 1388
Bso31I	GGTCTC	1 cut(s)	2043
BsoBI	CYCGRG	1 cut(s)	1098
Bsp119I	TTCGAA	1 cut(s)	1454
Bsp1286I	GDGCHC	4 cut(s)	175, 444, 1098, 1797
Bsp13I	TCCGGA	1 cut(s)	153
Bsp19I	CCATGG	1 cut(s)	1185
BspACI	CCGC	8 cut(s)	488, 557, 560, 905, 1031, 1314, 1717, 1848
BspANI	GGCC	3 cut(s)	487, 505, 1388
BspCNI	CTCAG	6 cut(s)	78, 114, 613, 923, 1860, 1924
BspDI	ATCGAT	2 cut(s)	1288, 1959
BspEI	TCCGGA	1 cut(s)	153
BspFNI	CGCG	2 cut(s)	907, 1155
BspHI	TCATGA	1 cut(s)	891
BspLI	GGNNCC	3 cut(s)	525, 1559, 1794
BspMI	ACCTGC	1 cut(s)	1701
BspPI	GGATC	2 cut(s)	1900, 1979
BspT104I	TTCGAA	1 cut(s)	1454
BspT107I	GGYRCC	2 cut(s)	523, 1792
BspTNI	GGTCTC	1 cut(s)	2043
BsrDI	GCAATG	1 cut(s)	163
BsrI	ACTGG	1 cut(s)	537
BssECI	CCNNGG	3 cut(s)	196, 1185, 1561
BssNI	GRCGYC	3 cut(s)	140, 425, 524
BssSI	CACGAG	2 cut(s)	443, 1162
BssT1I	CCWWGG	1 cut(s)	1185
Bst2BI	CACGAG	2 cut(s)	443, 1162
Bst2UI	CCWGG	4 cut(s)	197, 1563, 1661, 2087
Bst4CI	ACNGT	2 cut(s)	1557, 1982
Bst6I	CTCTTC	1 cut(s)	1251
BstACI	GRCGYC	3 cut(s)	140, 425, 524
BstBAI	YACGTR	1 cut(s)	2044
BstBI	TTCGAA	1 cut(s)	1454
BstC8I	GCNNGC	3 cut(s)	1267, 1325, 1681
BstDEI	CTNAG	8 cut(s)	86, 101, 621, 910, 970, 1270, 1868, 1911
BstDSI	CCRYGG	1 cut(s)	1185
BstF5I	GGATG	4 cut(s)	583, 1039, 1282, 1618
BstFNI	CGCG	2 cut(s)	907, 1155
BstH2I	RGCGCY	2 cut(s)	527, 1069
BstHHI	GCGC	3 cut(s)	526, 909, 1068
BstMAI	GTCTC	5 cut(s)	425, 611, 751, 1869, 2043
BstMWI	GCNNNNNNNGC	9 cut(s)	76, 96, 465, 856, 906, 1028, 1093, 1144, 1187
BstNI	CCWGG	4 cut(s)	197, 1563, 1661, 2087
BstNSI	RCATGY	2 cut(s)	670, 958
BstSCI	CCNGG	5 cut(s)	195, 253, 1561, 1659, 2085
BstSFI	CTRYAG	1 cut(s)	1978
BstSLI	GKGCMC	3 cut(s)	444, 1098, 1797
BstUI	CGCG	2 cut(s)	907, 1155
BstV1I	GCAGC	5 cut(s)	179, 477, 639, 1156, 1168
BstV2I	GAAGAC	3 cut(s)	669, 1338, 1559
BstX2I	RGATCY	2 cut(s)	913, 1905
BstYI	RGATCY	2 cut(s)	913, 1905
Bsu15I	ATCGAT	2 cut(s)	1288, 1959
BsuRI	GGCC	3 cut(s)	487, 505, 1388
BsuTUI	ATCGAT	2 cut(s)	1288, 1959
BtgI	CCRYGG	1 cut(s)	1185
BtsCI	GGATG	4 cut(s)	583, 1039, 1282, 1618
BtsIMutI	CAGTG	2 cut(s)	544, 1987
BveI	ACCTGC	1 cut(s)	1701
Cac8I	GCNNGC	3 cut(s)	1267, 1325, 1681
CaiI	CAGNNNCTG	1 cut(s)	620
CciI	TCATGA	1 cut(s)	891
CfoI	GCGC	3 cut(s)	526, 909, 1068
ClaI	ATCGAT	2 cut(s)	1288, 1959
CseI	GACGC	1 cut(s)	129
Csp6I	GTAC	3 cut(s)	211, 1603, 2067
CviAII	CATG	6 cut(s)	667, 892, 955, 1186, 1464, 1916
CviQI	GTAC	3 cut(s)	211, 1603, 2067
DdeI	CTNAG	8 cut(s)	86, 101, 621, 910, 970, 1270, 1868, 1911
DinI	GGCGCC	1 cut(s)	525
EaeI	YGGCCR	1 cut(s)	485
Eam1104I	CTCTTC	1 cut(s)	1251
EarI	CTCTTC	1 cut(s)	1251
EciI	GGCGGA	1 cut(s)	575
Ecl136II	GAGCTC	1 cut(s)	173
Eco130I	CCWWGG	1 cut(s)	1185
Eco147I	AGGCCT	1 cut(s)	1388
Eco24I	GRGCYC	1 cut(s)	175
Eco31I	GGTCTC	1 cut(s)	2043
Eco32I	GATATC	1 cut(s)	708
Eco53kI	GAGCTC	1 cut(s)	173
Eco57I	CTGAAG	1 cut(s)	76
Eco72I	CACGTG	1 cut(s)	2044
Eco88I	CYCGRG	1 cut(s)	1098
EcoICRI	GAGCTC	1 cut(s)	173
EcoRII	CCWGG	4 cut(s)	195, 1561, 1659, 2085
EcoRV	GATATC	1 cut(s)	708
EcoT14I	CCWWGG	1 cut(s)	1185
EcoT38I	GRGCYC	1 cut(s)	175
EgeI	GGCGCC	1 cut(s)	525
EheI	GGCGCC	1 cut(s)	525
ErhI	CCWWGG	1 cut(s)	1185
Esp3I	CGTCTC	1 cut(s)	425
FaeI	CATG	6 cut(s)	670, 895, 958, 1189, 1467, 1919
FaqI	GGGAC	1 cut(s)	1208
FatI	CATG	6 cut(s)	666, 891, 954, 1185, 1463, 1915
FauI	CCCGC	1 cut(s)	1855
FbaI	TGATCA	3 cut(s)	829, 1006, 1726
FblI	GTMKAC	1 cut(s)	605
Fnu4HI	GCNGC	8 cut(s)	193, 466, 488, 558, 653, 1145, 1182, 1718
FokI	GGATG	4 cut(s)	590, 1046, 1289, 1625
FriOI	GRGCYC	1 cut(s)	175
Fsp4HI	GCNGC	8 cut(s)	193, 466, 488, 558, 653, 1145, 1182, 1718
FspBI	CTAG	4 cut(s)	590, 761, 1019, 1856
GlaI	GCGC	3 cut(s)	525, 908, 1067
GluI	GCNGC	8 cut(s)	193, 466, 488, 558, 653, 1145, 1182, 1718
HaeII	RGCGCY	2 cut(s)	527, 1069
HaeIII	GGCC	3 cut(s)	487, 505, 1388
HapII	CCGG	4 cut(s)	154, 254, 527, 1110
HgaI	GACGC	1 cut(s)	129
HhaI	GCGC	3 cut(s)	526, 909, 1068
Hin1I	GRCGYC	3 cut(s)	140, 425, 524
Hin1II	CATG	6 cut(s)	670, 895, 958, 1189, 1467, 1919
Hin6I	GCGC	3 cut(s)	524, 907, 1066
HinP1I	GCGC	3 cut(s)	524, 907, 1066
HincII	GTYRAC	2 cut(s)	606, 1041
HindII	GTYRAC	2 cut(s)	606, 1041
HindIII	AAGCTT	3 cut(s)	345, 1267, 1837
HpaII	CCGG	4 cut(s)	154, 254, 527, 1110
Hpy166II	GTNNAC	5 cut(s)	229, 606, 1041, 1115, 1441
Hpy8I	GTNNAC	5 cut(s)	229, 606, 1041, 1115, 1441
Hpy99I	CGWCG	4 cut(s)	145, 430, 439, 496
HpyAV	CCTTC	8 cut(s)	29, 195, 263, 382, 625, 861, 947, 1116
HpyCH4III	ACNGT	2 cut(s)	1557, 1982
HpyCH4IV	ACGT	6 cut(s)	309, 425, 921, 1298, 1749, 2043
HpyF10VI	GCNNNNNNNGC	9 cut(s)	76, 96, 465, 856, 906, 1028, 1093, 1144, 1187
HpyF3I	CTNAG	8 cut(s)	86, 101, 621, 910, 970, 1270, 1868, 1911
HpySE526I	ACGT	6 cut(s)	309, 425, 921, 1298, 1749, 2043
Hsp92I	GRCGYC	3 cut(s)	140, 425, 524
Hsp92II	CATG	6 cut(s)	670, 895, 958, 1189, 1467, 1919
HspAI	GCGC	3 cut(s)	524, 907, 1066
KasI	GGCGCC	1 cut(s)	523
Kpn2I	TCCGGA	1 cut(s)	153
Ksp22I	TGATCA	3 cut(s)	829, 1006, 1726
LmnI	GCTCC	5 cut(s)	110, 275, 363, 451, 1480
Lsp1109I	GCAGC	5 cut(s)	179, 477, 639, 1156, 1168
LweI	GCATC	6 cut(s)	837, 1018, 1483, 1704, 1951, 2084
MaeI	CTAG	4 cut(s)	590, 761, 1019, 1856
MaeII	ACGT	6 cut(s)	309, 425, 921, 1298, 1749, 2043
MaeIII	GTNAC	3 cut(s)	263, 326, 1059
MboII	GAAGA	6 cut(s)	51, 674, 793, 1238, 1338, 1559
MfeI	CAATTG	2 cut(s)	1139, 1989
MflI	RGATCY	2 cut(s)	913, 1905
MhlI	GDGCHC	4 cut(s)	175, 444, 1098, 1797
MluCI	AATT	6 cut(s)	647, 881, 944, 1126, 1139, 1989
Mly113I	GGCGCC	1 cut(s)	524
MlyI	GAGTC	5 cut(s)	485, 1110, 1174, 1762, 2093
MmeI	TCCRAC	2 cut(s)	207, 408
MroI	TCCGGA	1 cut(s)	153
MroXI	GAANNNNTTC	3 cut(s)	813, 1245, 2053
MseI	TTAA	5 cut(s)	924, 1175, 1538, 1668, 1953
MslI	CAYNNNNRTG	1 cut(s)	576
MspI	CCGG	4 cut(s)	154, 254, 527, 1110
MspR9I	CCNGG	5 cut(s)	197, 255, 1563, 1661, 2087
MunI	CAATTG	2 cut(s)	1139, 1989
Mva1269I	GAATGC	2 cut(s)	1325, 1720
MvaI	CCWGG	4 cut(s)	197, 1563, 1661, 2087
MvnI	CGCG	2 cut(s)	907, 1155
MwoI	GCNNNNNNNGC	9 cut(s)	76, 96, 465, 856, 906, 1028, 1093, 1144, 1187
NarI	GGCGCC	1 cut(s)	524
NciI	CCSGG	1 cut(s)	255
NcoI	CCATGG	1 cut(s)	1185
NlaIII	CATG	6 cut(s)	670, 895, 958, 1189, 1467, 1919
NlaIV	GGNNCC	3 cut(s)	525, 1559, 1794
NmuCI	GTSAC	2 cut(s)	263, 326
NspI	RCATGY	2 cut(s)	670, 958
NspV	TTCGAA	1 cut(s)	1454
PagI	TCATGA	1 cut(s)	891
PceI	AGGCCT	1 cut(s)	1388
PciI	ACATGT	1 cut(s)	666
PcsI	WCGNNNNNNNCGW	1 cut(s)	431
PctI	GAATGC	2 cut(s)	1325, 1720
PdmI	GAANNNNTTC	3 cut(s)	813, 1245, 2053
PfeI	GAWTC	9 cut(s)	177, 248, 1015, 1229, 1241, 1597, 2003, 2054, 2090
PflMI	CCANNNNNTGG	1 cut(s)	1256
PkrI	GCNGC	8 cut(s)	194, 467, 489, 559, 654, 1146, 1183, 1719
PleI	GAGTC	5 cut(s)	484, 1110, 1173, 1761, 2093
PluTI	GGCGCC	1 cut(s)	527
PmaCI	CACGTG	1 cut(s)	2044
PmlI	CACGTG	1 cut(s)	2044
PpsI	GAGTC	5 cut(s)	484, 1110, 1173, 1761, 2093
Ppu21I	YACGTR	1 cut(s)	2044
PscI	ACATGT	1 cut(s)	666
Psp124BI	GAGCTC	1 cut(s)	175
Psp6I	CCWGG	4 cut(s)	195, 1561, 1659, 2085
PspCI	CACGTG	1 cut(s)	2044
PspGI	CCWGG	4 cut(s)	195, 1561, 1659, 2085
PspN4I	GGNNCC	3 cut(s)	525, 1559, 1794
PsrI	GAACNNNNNNTAC	2 cut(s)	1920, 1952
PstNI	CAGNNNCTG	1 cut(s)	620
PsuI	RGATCY	2 cut(s)	913, 1905
RsaI	GTAC	3 cut(s)	212, 1604, 2068
RsaNI	GTAC	3 cut(s)	211, 1603, 2067
RseI	CAYNNNNRTG	1 cut(s)	576
SacI	GAGCTC	1 cut(s)	175
SalI	GTCGAC	1 cut(s)	604
SaqAI	TTAA	5 cut(s)	924, 1175, 1538, 1668, 1953
SatI	GCNGC	8 cut(s)	193, 466, 488, 558, 653, 1145, 1182, 1718
SchI	GAGTC	5 cut(s)	485, 1110, 1174, 1762, 2093
ScrFI	CCNGG	5 cut(s)	197, 255, 1563, 1661, 2087
SduI	GDGCHC	4 cut(s)	175, 444, 1098, 1797
SfaNI	GCATC	6 cut(s)	837, 1018, 1483, 1704, 1951, 2084
SfcI	CTRYAG	1 cut(s)	1978
SfoI	GGCGCC	1 cut(s)	525
SfuI	TTCGAA	1 cut(s)	1454
SmiMI	CAYNNNNRTG	1 cut(s)	576
SmlI	CTYRAG	2 cut(s)	65, 1801
SmoI	CTYRAG	2 cut(s)	65, 1801
Sse9I	AATT	6 cut(s)	647, 881, 944, 1126, 1139, 1989
SseBI	AGGCCT	1 cut(s)	1388
SsiI	CCGC	8 cut(s)	488, 557, 560, 905, 1031, 1314, 1717, 1848
SspDI	GGCGCC	1 cut(s)	523
SspI	AATATT	1 cut(s)	1173
SspMI	CTAG	4 cut(s)	590, 761, 1019, 1856
SstI	GAGCTC	1 cut(s)	175
StuI	AGGCCT	1 cut(s)	1388
StyD4I	CCNGG	5 cut(s)	195, 253, 1561, 1659, 2085
StyI	CCWWGG	1 cut(s)	1185
TaaI	ACNGT	2 cut(s)	1557, 1982
TaiI	ACGT	6 cut(s)	312, 428, 924, 1301, 1752, 2046
TasI	AATT	6 cut(s)	647, 881, 944, 1126, 1139, 1989
TatI	WGTACW	1 cut(s)	1602
TauI	GCSGC	3 cut(s)	490, 560, 1720
TfiI	GAWTC	9 cut(s)	177, 248, 1015, 1229, 1241, 1597, 2003, 2054, 2090
Tru1I	TTAA	5 cut(s)	924, 1175, 1538, 1668, 1953
Tru9I	TTAA	5 cut(s)	924, 1175, 1538, 1668, 1953
TscAI	CASTG	2 cut(s)	544, 1987
TseFI	GTSAC	2 cut(s)	263, 326
TseI	GCWGC	5 cut(s)	192, 465, 652, 1144, 1181
Tsp45I	GTSAC	2 cut(s)	263, 326
TspDTI	ATGAA	3 cut(s)	567, 644, 1879
TspGWI	ACGGA	1 cut(s)	345
TspRI	CASTG	2 cut(s)	544, 1987
Van91I	CCANNNNNTGG	1 cut(s)	1256
XapI	RAATTY	1 cut(s)	1126
XceI	RCATGY	2 cut(s)	670, 958
XmiI	GTMKAC	1 cut(s)	605
XmnI	GAANNNNTTC	3 cut(s)	813, 1245, 2053
XspI	CTAG	4 cut(s)	590, 761, 1019, 1856
ZraI	GACGTC	1 cut(s)	426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.