Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006089

Physical Location & Seq

Genomic Coordinates Forward (+)

839754 .. 841900

Gene Structure 2147 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G03406.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 792 bp

ATGCGAGCAATTCACATCGTGACGACGCGATTGAGCTCCAGCTTTATTAGGCCTGTTCTTCTTGACTTCGTTTCTTGTTCGCCTCTACGCCAGTTCTCTACCCCTAGGCGCTTTATCTGTGCCGCGAGCAAGGTCAATGGAGGAGGCAGGTCAGGTTCGATTGTTGCTCCATTGGTAGTGAATGAAGAATTTCAGAACATCGATGTCAATCCTCCTAAAGGAACTCGTGATTTCCCTCCTGAGGATATGCGACTCCGCAACTGGCTCTTCAACCACTTCAAAGAGGTCTCACGGTTATTTGGGTATGAGGAAGTGGATTTTCCAGTACTGGAGACGGAGGCATTGTTCATCAGAAAAGCTGGGGAGGAGATTAGAGACCAACTTTACTGCTTTGAAGATAGGGGTAATCGCCGTGTAGCATTGAGGCCTGAGCTTACACCTTCTTTAGCGAGGCTTGTCATACAGAAAGGAAAATCTGTTTCCCTTCCATTGAAGTGGTTTGCTGTTGGGCAGTGTTGGAGATATGAGAGAATGACACGAGGAAGGCGACGTGAGCATTACCAGTGGAATATGGATATTATTGGTGTGCCTGAAGTTACAGCTGAAGCAGAACTAATTTCATCTATAATGACATTCTTCAAGCGAATTGGAATCACTGCATCAGATGTGGGTTTTAAGGTTTCAAGCCGCAAGGTTTTACAAGAATTTTTAAAAAAGTATGGTGTACCCGAAGACCTGTTTGGAAGAGTTTGCTTCATTATAGACAAGGTTAGCAATCTAAACATCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004821 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006427 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_His

Protein Analysis

263

Amino Acids

30.31

Weight (kDa)

9.58

Isoelectric Point (pI)

48.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_His	PF13393	74 - 246	2.3e-23	Histidyl-tRNA synthetase
tRNA-synt_2b	PF00587	129 - 221	4.8e-11	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46100 AT5G03406
fragaria_vesca	FvH4_3g29130 FvH4_3g29130 FvH4_3g29130
malus_domestica	MD04G1093200.v1.1 MD12G1112900.v1.1
prunus_persica	Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1
pyrus_communis	pycom04g08820
rosa_chinensis	RchiOBHm_Chr3g0470291
rosa_laevigata	RLG00000024282
rosa_multiflora	Rmu_sc0011073.1_g000001 Rmu_sc0019034.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410990
rosa_rugosa	Rorug03G0107800 Rorug03G0107900
rosa_samantha	Rh3AG157600 Rh3BG181800 Rh3CG172500 Rh3DG193100
rosa_wichuraiana	Rw3G014990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	138
AccII	CGCG	2 cut(s)	28, 125
AciI	CCGC	3 cut(s)	123, 256, 688
AcsI	RAATTY	2 cut(s)	188, 704
AcuI	CTGAAG	2 cut(s)	612, 624
AdeI	CACNNNGTG	1 cut(s)	19
AfaI	GTAC	2 cut(s)	327, 726
AfiI	CCNNNNNNNGG	2 cut(s)	218, 241
AgsI	TTSAA	6 cut(s)	271, 280, 395, 493, 640, 684
AjiI	CACGTC	1 cut(s)	551
AjuI	GAANNNNNNNTTGG	2 cut(s)	723, 755
AloI	GAACNNNNNNTCC	2 cut(s)	329, 361
AluBI	AGCT	5 cut(s)	36, 42, 359, 433, 602
AluI	AGCT	5 cut(s)	36, 42, 359, 433, 602
Alw21I	GWGCWC	1 cut(s)	38
Alw26I	GTCTC	3 cut(s)	292, 326, 369
AoxI	GGCC	2 cut(s)	50, 425
ApoI	RAATTY	2 cut(s)	188, 704
Asp700I	GAANNNNTTC	1 cut(s)	189
AspA2I	CCTAGG	1 cut(s)	104
AspLEI	GCGC	1 cut(s)	111
AvrII	CCTAGG	1 cut(s)	104
AxyI	CCTNAGG	1 cut(s)	240
BanII	GRGCYC	1 cut(s)	38
BauI	CACGAG	2 cut(s)	225, 537
BbsI	GAAGAC	1 cut(s)	738
Bbv12I	GWGCWC	1 cut(s)	38
BceAI	ACGGC	1 cut(s)	396
BcoDI	GTCTC	3 cut(s)	292, 326, 369
BfaI	CTAG	1 cut(s)	105
BfoI	RGCGCY	1 cut(s)	112
BfuAI	ACCTGC	1 cut(s)	138
BisI	GCNGC	2 cut(s)	123, 688
BlnI	CCTAGG	1 cut(s)	104
BlsI	GCNGC	2 cut(s)	124, 689
BmcAI	AGTACT	1 cut(s)	327
BmgBI	CACGTC	1 cut(s)	551
BmsI	GCATC	1 cut(s)	668
BpiI	GAAGAC	1 cut(s)	738
BpmI	CTGGAG	2 cut(s)	22, 350
Bpu10I	CCTNAGC	1 cut(s)	429
Bsa29I	ATCGAT	1 cut(s)	201
BsaBI	GATNNNNATC	1 cut(s)	207
BsaI	GGTCTC	2 cut(s)	292, 369
BsaJI	CCNNGG	1 cut(s)	104
BsaXI	ACNNNNNCTCC	2 cut(s)	329, 359
Bsc4I	CCNNNNNNNGG	2 cut(s)	218, 241
Bse1I	ACTGG	5 cut(s)	91, 266, 323, 333, 562
Bse21I	CCTNAGG	1 cut(s)	240
Bse8I	GATNNNNATC	1 cut(s)	207
BseCI	ATCGAT	1 cut(s)	201
BseDI	CCNNGG	1 cut(s)	104
BseJI	GATNNNNATC	1 cut(s)	207
BseLI	CCNNNNNNNGG	2 cut(s)	218, 241
BseMII	CTCAG	2 cut(s)	231, 420
BseNI	ACTGG	5 cut(s)	91, 266, 323, 333, 562
BseRI	GAGGAG	2 cut(s)	156, 380
BseYI	CCCAGC	1 cut(s)	359
Bsh1236I	CGCG	2 cut(s)	28, 125
BshFI	GGCC	2 cut(s)	52, 427
BshVI	ATCGAT	1 cut(s)	201
BsiHKAI	GWGCWC	1 cut(s)	38
BslI	CCNNNNNNNGG	2 cut(s)	218, 241
BsmAI	GTCTC	3 cut(s)	292, 326, 369
BsmBI	CGTCTC	1 cut(s)	326
BsnI	GGCC	2 cut(s)	52, 427
Bso31I	GGTCTC	2 cut(s)	292, 369
Bsp1286I	GDGCHC	1 cut(s)	38
BspACI	CCGC	3 cut(s)	123, 256, 688
BspANI	GGCC	2 cut(s)	52, 427
BspCNI	CTCAG	2 cut(s)	232, 421
BspDI	ATCGAT	1 cut(s)	201
BspFNI	CGCG	2 cut(s)	28, 125
BspMI	ACCTGC	1 cut(s)	138
BspQI	GCTCTTC	1 cut(s)	272
BspTNI	GGTCTC	2 cut(s)	292, 369
BsrI	ACTGG	5 cut(s)	91, 266, 323, 333, 562
BssECI	CCNNGG	1 cut(s)	104
BssSI	CACGAG	2 cut(s)	225, 537
BssT1I	CCWWGG	1 cut(s)	104
Bst2BI	CACGAG	2 cut(s)	225, 537
Bst4CI	ACNGT	1 cut(s)	294
Bst6I	CTCTTC	2 cut(s)	272, 739
BstC8I	GCNNGC	2 cut(s)	6, 127
BstDEI	CTNAG	2 cut(s)	240, 429
BstENI	CCTNNNNNAGG	1 cut(s)	216
BstFNI	CGCG	2 cut(s)	28, 125
BstH2I	RGCGCY	1 cut(s)	112
BstHHI	GCGC	1 cut(s)	111
BstMAI	GTCTC	3 cut(s)	292, 326, 369
BstMWI	GCNNNNNNNGC	1 cut(s)	553
BstUI	CGCG	2 cut(s)	28, 125
BstV2I	GAAGAC	1 cut(s)	738
BstXI	CCANNNNNNTGG	1 cut(s)	495
Bsu15I	ATCGAT	1 cut(s)	201
Bsu36I	CCTNAGG	1 cut(s)	240
BsuRI	GGCC	2 cut(s)	52, 427
BsuTUI	ATCGAT	1 cut(s)	201
BtrI	CACGTC	1 cut(s)	551
BtsI	GCAGTG	2 cut(s)	518, 654
BtsIMutI	CAGTG	3 cut(s)	518, 569, 654
BveI	ACCTGC	1 cut(s)	138
Cac8I	GCNNGC	2 cut(s)	6, 127
CfoI	GCGC	1 cut(s)	111
ClaI	ATCGAT	1 cut(s)	201
CseI	GACGC	1 cut(s)	34
Csp6I	GTAC	2 cut(s)	326, 725
CviQI	GTAC	2 cut(s)	326, 725
DdeI	CTNAG	2 cut(s)	240, 429
DraI	TTTAAA	1 cut(s)	711
DraIII	CACNNNGTG	1 cut(s)	19
Eam1104I	CTCTTC	2 cut(s)	272, 739
EarI	CTCTTC	2 cut(s)	272, 739
Ecl136II	GAGCTC	1 cut(s)	36
Eco130I	CCWWGG	1 cut(s)	104
Eco147I	AGGCCT	2 cut(s)	52, 427
Eco24I	GRGCYC	1 cut(s)	38
Eco31I	GGTCTC	2 cut(s)	292, 369
Eco53kI	GAGCTC	1 cut(s)	36
Eco57I	CTGAAG	2 cut(s)	612, 624
Eco81I	CCTNAGG	1 cut(s)	240
EcoICRI	GAGCTC	1 cut(s)	36
EcoNI	CCTNNNNNAGG	1 cut(s)	216
EcoT14I	CCWWGG	1 cut(s)	104
EcoT38I	GRGCYC	1 cut(s)	38
ErhI	CCWWGG	1 cut(s)	104
Esp3I	CGTCTC	1 cut(s)	326
FaiI	YATR	8 cut(s)	248, 306, 461, 525, 572, 626, 720, 761
Fnu4HI	GCNGC	2 cut(s)	123, 688
FriOI	GRGCYC	1 cut(s)	38
Fsp4HI	GCNGC	2 cut(s)	123, 688
FspBI	CTAG	1 cut(s)	105
GlaI	GCGC	1 cut(s)	110
GluI	GCNGC	2 cut(s)	123, 688
GsaI	CCCAGC	1 cut(s)	363
GsuI	CTGGAG	2 cut(s)	22, 350
HaeII	RGCGCY	1 cut(s)	112
HaeIII	GGCC	2 cut(s)	52, 427
HgaI	GACGC	1 cut(s)	34
HhaI	GCGC	1 cut(s)	111
Hin6I	GCGC	1 cut(s)	109
HinP1I	GCGC	1 cut(s)	109
HinfI	GANTC	2 cut(s)	252, 651
Hpy166II	GTNNAC	1 cut(s)	725
Hpy188I	TCNGA	3 cut(s)	195, 353, 664
Hpy188III	TCNNGA	4 cut(s)	19, 62, 227, 239
Hpy8I	GTNNAC	1 cut(s)	725
Hpy99I	CGWCG	2 cut(s)	28, 552
HpyAV	CCTTC	3 cut(s)	450, 494, 537
HpyCH4III	ACNGT	1 cut(s)	294
HpyCH4IV	ACGT	1 cut(s)	550
HpyCH4V	TGCA	1 cut(s)	659
HpyF10VI	GCNNNNNNNGC	1 cut(s)	553
HpyF3I	CTNAG	2 cut(s)	240, 429
HpySE526I	ACGT	1 cut(s)	550
HspAI	GCGC	1 cut(s)	109
LguI	GCTCTTC	1 cut(s)	272
LmnI	GCTCC	2 cut(s)	41, 172
LweI	GCATC	1 cut(s)	668
MaeI	CTAG	1 cut(s)	105
MaeII	ACGT	1 cut(s)	550
MaeIII	GTNAC	2 cut(s)	19, 595
MboII	GAAGA	7 cut(s)	50, 197, 259, 407, 628, 743, 756
MhlI	GDGCHC	1 cut(s)	38
MluCI	AATT	6 cut(s)	9, 188, 615, 645, 704, 787
MlyI	GAGTC	1 cut(s)	246
MmeI	TCCRAC	1 cut(s)	497
MroXI	GAANNNNTTC	1 cut(s)	189
MseI	TTAA	3 cut(s)	675, 710, 790
MslI	CAYNNNNRTG	1 cut(s)	493
MspA1I	CMGCKG	1 cut(s)	602
MvnI	CGCG	2 cut(s)	28, 125
MwoI	GCNNNNNNNGC	1 cut(s)	553
NmuCI	GTSAC	1 cut(s)	19
PceI	AGGCCT	2 cut(s)	52, 427
PciSI	GCTCTTC	1 cut(s)	272
PcsI	WCGNNNNNNNCGW	1 cut(s)	544
PdmI	GAANNNNTTC	1 cut(s)	189
PfeI	GAWTC	1 cut(s)	651
PkrI	GCNGC	2 cut(s)	124, 689
PleI	GAGTC	1 cut(s)	246
PpsI	GAGTC	1 cut(s)	246
Psp124BI	GAGCTC	1 cut(s)	38
PspFI	CCCAGC	1 cut(s)	359
PvuII	CAGCTG	1 cut(s)	602
RsaI	GTAC	2 cut(s)	327, 726
RsaNI	GTAC	2 cut(s)	326, 725
RseI	CAYNNNNRTG	1 cut(s)	493
SacI	GAGCTC	1 cut(s)	38
SapI	GCTCTTC	1 cut(s)	272
SaqAI	TTAA	3 cut(s)	675, 710, 790
SatI	GCNGC	2 cut(s)	123, 688
ScaI	AGTACT	1 cut(s)	327
SchI	GAGTC	1 cut(s)	246
SduI	GDGCHC	1 cut(s)	38
SfaNI	GCATC	1 cut(s)	668
SmiMI	CAYNNNNRTG	1 cut(s)	493
Sse9I	AATT	6 cut(s)	9, 188, 615, 645, 704, 787
SseBI	AGGCCT	2 cut(s)	52, 427
SsiI	CCGC	3 cut(s)	123, 256, 688
SspMI	CTAG	1 cut(s)	105
SstI	GAGCTC	1 cut(s)	38
StuI	AGGCCT	2 cut(s)	52, 427
StyI	CCWWGG	1 cut(s)	104
TaaI	ACNGT	1 cut(s)	294
TaiI	ACGT	1 cut(s)	553
TaqI	TCGA	2 cut(s)	158, 201
TasI	AATT	6 cut(s)	9, 188, 615, 645, 704, 787
TatI	WGTACW	1 cut(s)	325
TauI	GCSGC	2 cut(s)	125, 690
TfiI	GAWTC	1 cut(s)	651
Tru1I	TTAA	3 cut(s)	675, 710, 790
Tru9I	TTAA	3 cut(s)	675, 710, 790
TscAI	CASTG	3 cut(s)	518, 569, 661
TseFI	GTSAC	1 cut(s)	19
Tsp45I	GTSAC	1 cut(s)	19
TspDTI	ATGAA	4 cut(s)	198, 337, 609, 745
TspGWI	ACGGA	1 cut(s)	350
TspRI	CASTG	3 cut(s)	518, 569, 661
XagI	CCTNNNNNAGG	1 cut(s)	216
XapI	RAATTY	2 cut(s)	188, 704
XmaJI	CCTAGG	1 cut(s)	104
XmnI	GAANNNNTTC	1 cut(s)	189
XspI	CTAG	1 cut(s)	105
ZrmI	AGTACT	1 cut(s)	327

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.