Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006089

Physical Location & Seq

Genomic Coordinates Reverse (-)

16821005 .. 16835041

Gene Structure 14037 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1093200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGCTCTCTGCAGTCTCGTCTTCCCTCCTTATCTTCCACAAGCCCTTCACTCGCGCTCTTCGACTTCCCCTCCCCAAACCCCCTCTCACCCCAAACCCTAGACCCCTCTCTTTACTCTCCCCGGCCGCTTCTTCTCTCACCCCCAATGCCACCGGTGGCGGTCGCATCGGGGCTCTATCTCCTGCGCCGGCCACCGATGACCTCCAGAAGGTCGACGTTAACCCTCCCAAGGGCACCAGGGACTTCCCGCCAGAAGATATGCGCCTCCGCAACTGGCTCTTCTGCAATTTCAAAGAGGTCTCGCGGCTGTTCGGCTTCGAAGAGGTCGACTTTCCGGTTCTCGAGACTGAGGCGCTGTTTATCAGAAAAGCAGGGGAGGAGATTCGTGACCAGCTGTATTGCTTCGAAGATCGAGGCAATCGTCGCGTCGCGTTGAGGCCGGAGCTCACTCCTTCTTTGGCCAGGCTGGTGATACAGAAAGGAAAATCTCTGTCCCTGCCATTGAAATGGTTTGCCGTTGGGCAGTGTTGGCGGTACGAGAGGATGACAAGGGGGCGGCGTCGGGAGCATTACCAGTGGAATATGGATATTATTGGTGTACCTGAGGTTACGGCTGAAGCAGAACTTATTTCTTCAATTGTCACTTTCCTCAAGCGATTAGGAATTACGGCATCAGATGTTGGATTTAAGGTTTCAAGTCGTAAGGTTTTACAAGAAGTACTGAGGCGCTATTCCATACCCGAATCTTTATTTGGCAAAGTATGCATCATCATAGACAAGATAGAGAAGATTCCAGTGGATGATATAAAAAATGAGTTGAAATCTGCTGGTGTATCAGAAGAGGCTATTGAACAGTTATTGCAAATTCTTTCCATAAAGTCATTGACAAAGCTGGAAGAGGTTCTTGGAGATGCTGGGGAAGCAGTTGCTGATTTGAAACAACTATTTTCTCTTTCTGACAAATTTGGTTATTCTGAATGGATCCAGTTTGATGCTTCTATTGTTCGTGGCCTTGCCTACTACACTGGTATTGTTTTCGAGGGTTTTGATAGAGGAGGAAAGTTACGAGCCATTTGTGGTGGTGGGCGATATGACCGCTTGCTTTCTACTTTTGGTGGTGATGACATTCCGGCTTGTGGATTTGGATTTGGTGATGCTGTCATAGTGGAATTGCTTAAGGAGAAGGGCCTTCTGCCGGAACTCAGCCTGCAAGTAGAGAACATTGTGTGTGCTCTTGATCCTGGACTTAAAGAAGCAGCTGCAACAGTTGCCACCATACTCAGGGGGAAAGGTCAAAGTGTTGATTTAGTCTTGGAGAACAAACCGCTTAAATGGGTATTCAAGCGTGCAGCACGGATAAATGCTAATAGGTTGATATTGGTGGGAGATTCAGAGTGGCAGAGGGGTATGGTGAGTGTGAAAGTCCTTTCTTCTGGTGAACAACATGAGATCAAACTTGATGAACTAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004821 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006427 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_His

Protein Analysis

491

Amino Acids

54.44

Weight (kDa)

8.04

Isoelectric Point (pI)

39.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_His	PF13393	78 - 382	5.6e-43	Histidyl-tRNA synthetase
tRNA-synt_2b	PF00587	133 - 229	2.7e-10	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	410 - 489	1.2e-09	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46100 AT5G03406
fragaria_vesca	FvH4_3g29130 FvH4_3g29130 FvH4_3g29130
malus_domestica	MD04G1093200.v1.1 MD12G1112900.v1.1
prunus_persica	Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1
pyrus_communis	pycom04g08820
rosa_chinensis	RchiOBHm_Chr3g0470291
rosa_laevigata	RLG00000024282
rosa_multiflora	Rmu_sc0011073.1_g000001 Rmu_sc0019034.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410990
rosa_rugosa	Rorug03G0107800 Rorug03G0107900
rosa_samantha	Rh3AG157600 Rh3BG181800 Rh3CG172500 Rh3DG193100
rosa_wichuraiana	Rw3G014990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	233
AccI	GTMKAC	2 cut(s)	213, 327
AccII	CGCG	4 cut(s)	54, 304, 426, 431
AciI	CCGC	9 cut(s)	126, 159, 248, 268, 304, 532, 556, 1096, 1325
AclWI	GGATC	3 cut(s)	976, 989, 1232
AcoI	YGGCCR	3 cut(s)	123, 189, 459
AcsI	RAATTY	2 cut(s)	864, 962
AcuI	CTGAAG	1 cut(s)	636
AcyI	GRCGYC	1 cut(s)	559
AfaI	GTAC	3 cut(s)	536, 600, 720
AfiI	CCNNNNNNNGG	6 cut(s)	208, 229, 230, 1136, 1195, 1281
AflII	CTTAAG	1 cut(s)	1175
AgeI	ACCGGT	1 cut(s)	152
AgsI	TTSAA	8 cut(s)	292, 505, 636, 696, 820, 851, 937, 1342
AjnI	CCWGG	3 cut(s)	236, 461, 1240
AjuI	GAANNNNNNNTTGG	4 cut(s)	735, 767, 888, 920
AluBI	AGCT	4 cut(s)	394, 445, 892, 1259
AluI	AGCT	4 cut(s)	394, 445, 892, 1259
Alw21I	GWGCWC	2 cut(s)	447, 1234
Alw26I	GTCTC	3 cut(s)	19, 304, 338
AlwI	GGATC	3 cut(s)	976, 989, 1232
AlwNI	CAGNNNCTG	1 cut(s)	929
Ama87I	CYCGRG	1 cut(s)	341
AoxI	GGCC	6 cut(s)	123, 189, 437, 459, 1009, 1186
ApeKI	GCWGC	3 cut(s)	1256, 1259, 1349
ApoI	RAATTY	2 cut(s)	864, 962
AsiGI	ACCGGT	1 cut(s)	152
Asp700I	GAANNNNTTC	1 cut(s)	900
AspLEI	GCGC	5 cut(s)	56, 187, 264, 355, 729
AspS9I	GGNCC	1 cut(s)	1186
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	7 cut(s)	79, 130, 481, 1130, 1163, 1423, 1448
AsuII	TTCGAA	2 cut(s)	318, 405
AvaI	CYCGRG	1 cut(s)	341
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	1 cut(s)	236
BalI	TGGCCA	1 cut(s)	461
BamHI	GGATCC	1 cut(s)	981
BanI	GGYRCC	1 cut(s)	233
BanII	GRGCYC	2 cut(s)	175, 447
BbsI	GAAGAC	1 cut(s)	12
Bbv12I	GWGCWC	2 cut(s)	447, 1234
BbvI	GCAGC	3 cut(s)	1246, 1268, 1361
BceAI	ACGGC	3 cut(s)	500, 627, 684
BciT130I	CCWGG	3 cut(s)	238, 463, 1242
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	3 cut(s)	19, 304, 338
BfaI	CTAG	2 cut(s)	99, 1466
BfmI	CTRYAG	1 cut(s)	9
BfoI	RGCGCY	2 cut(s)	356, 730
BfrI	CTTAAG	1 cut(s)	1175
BisI	GCNGC	6 cut(s)	126, 305, 557, 1257, 1260, 1350
BlsI	GCNGC	6 cut(s)	127, 306, 558, 1258, 1261, 1351
BmcAI	AGTACT	1 cut(s)	720
Bme1390I	CCNGG	4 cut(s)	122, 238, 463, 1242
BmeT110I	CYCGRG	1 cut(s)	341
BmgT120I	GGNCC	1 cut(s)	1186
BmiI	GGNNCC	2 cut(s)	235, 983
BmrFI	CCNGG	4 cut(s)	122, 238, 463, 1242
BmsI	GCATC	6 cut(s)	174, 680, 774, 901, 982, 1144
BpiI	GAAGAC	1 cut(s)	12
BpmI	CTGGAG	1 cut(s)	188
Bpu14I	TTCGAA	2 cut(s)	318, 405
BpuEI	CTTGAG	1 cut(s)	635
BpuMI	CCSGG	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	559
BsaI	GGTCTC	1 cut(s)	304
BsaJI	CCNNGG	3 cut(s)	120, 228, 237
BsaWI	WCCGGW	2 cut(s)	152, 334
BsaXI	ACNNNNNCTCC	2 cut(s)	54, 84
Bsc4I	CCNNNNNNNGG	6 cut(s)	208, 229, 230, 1136, 1195, 1281
Bse118I	RCCGGY	2 cut(s)	152, 187
Bse1I	ACTGG	5 cut(s)	278, 574, 794, 985, 1030
Bse21I	CCTNAGG	1 cut(s)	603
BseBI	CCWGG	3 cut(s)	238, 463, 1242
BseDI	CCNNGG	3 cut(s)	120, 228, 237
BseGI	GGATG	2 cut(s)	549, 805
BseLI	CCNNNNNNNGG	6 cut(s)	208, 229, 230, 1136, 1195, 1281
BseMII	CTCAG	5 cut(s)	339, 594, 713, 1216, 1294
BseNI	ACTGG	5 cut(s)	278, 574, 794, 985, 1030
BseRI	GAGGAG	2 cut(s)	392, 1068
BseSI	GKGCMC	1 cut(s)	236
BseX3I	CGGCCG	1 cut(s)	123
BseXI	GCAGC	3 cut(s)	1246, 1268, 1361
BseYI	CCCAGC	1 cut(s)	914
BsgI	GTGCAG	1 cut(s)	1368
Bsh1236I	CGCG	4 cut(s)	54, 304, 426, 431
Bsh1285I	CGRYCG	2 cut(s)	126, 163
BshFI	GGCC	6 cut(s)	125, 191, 439, 461, 1011, 1188
BshNI	GGYRCC	1 cut(s)	233
BshTI	ACCGGT	1 cut(s)	152
BsiEI	CGRYCG	2 cut(s)	126, 163
BsiHKAI	GWGCWC	2 cut(s)	447, 1234
BsiHKCI	CYCGRG	1 cut(s)	341
BsiSI	CCGG	7 cut(s)	122, 153, 188, 335, 440, 1130, 1196
BslFI	GGGAC	2 cut(s)	254, 478
BslI	CCNNNNNNNGG	6 cut(s)	208, 229, 230, 1136, 1195, 1281
BsmAI	GTCTC	3 cut(s)	19, 304, 338
BsmFI	GGGAC	2 cut(s)	254, 478
BsnI	GGCC	6 cut(s)	125, 191, 439, 461, 1011, 1188
Bso31I	GGTCTC	1 cut(s)	304
BsoBI	CYCGRG	1 cut(s)	341
Bsp119I	TTCGAA	2 cut(s)	318, 405
Bsp1286I	GDGCHC	4 cut(s)	175, 236, 447, 1234
Bsp143I	GATC	4 cut(s)	409, 981, 1237, 1449
BspACI	CCGC	9 cut(s)	126, 159, 248, 268, 304, 532, 556, 1096, 1325
BspANI	GGCC	6 cut(s)	125, 191, 439, 461, 1011, 1188
BspCNI	CTCAG	5 cut(s)	340, 595, 714, 1215, 1293
BspFNI	CGCG	4 cut(s)	54, 304, 426, 431
BspLI	GGNNCC	2 cut(s)	235, 983
BspMAI	CTGCAG	1 cut(s)	13
BspPI	GGATC	3 cut(s)	976, 989, 1232
BspQI	GCTCTTC	2 cut(s)	63, 284
BspT104I	TTCGAA	2 cut(s)	318, 405
BspT107I	GGYRCC	1 cut(s)	233
BspTI	CTTAAG	1 cut(s)	1175
BspTNI	GGTCTC	1 cut(s)	304
BsrFI	RCCGGY	2 cut(s)	152, 187
BsrI	ACTGG	5 cut(s)	278, 574, 794, 985, 1030
BssAI	RCCGGY	2 cut(s)	152, 187
BssECI	CCNNGG	3 cut(s)	120, 228, 237
BssMI	GATC	4 cut(s)	409, 981, 1237, 1449
BssNI	GRCGYC	1 cut(s)	559
BssT1I	CCWWGG	1 cut(s)	228
Bst2UI	CCWGG	3 cut(s)	238, 463, 1242
Bst4CI	ACNGT	2 cut(s)	855, 1267
Bst6I	CTCTTC	5 cut(s)	63, 284, 315, 834, 891
BstACI	GRCGYC	1 cut(s)	559
BstAFI	CTTAAG	1 cut(s)	1175
BstAPI	GCANNNNNTGC	2 cut(s)	762, 1268
BstBI	TTCGAA	2 cut(s)	318, 405
BstC8I	GCNNGC	4 cut(s)	189, 1100, 1208, 1347
BstDEI	CTNAG	5 cut(s)	348, 603, 722, 1202, 1280
BstENI	CCTNNNNNAGG	1 cut(s)	206
BstF5I	GGATG	2 cut(s)	549, 805
BstFNI	CGCG	4 cut(s)	54, 304, 426, 431
BstH2I	RGCGCY	2 cut(s)	356, 730
BstHHI	GCGC	5 cut(s)	56, 187, 264, 355, 729
BstKTI	GATC	4 cut(s)	412, 984, 1240, 1452
BstMAI	GTCTC	3 cut(s)	19, 304, 338
BstMBI	GATC	4 cut(s)	409, 981, 1237, 1449
BstMCI	CGRYCG	2 cut(s)	126, 163
BstMWI	GCNNNNNNNGC	6 cut(s)	423, 529, 565, 762, 920, 1268
BstNI	CCWGG	3 cut(s)	238, 463, 1242
BstSCI	CCNGG	4 cut(s)	120, 236, 461, 1240
BstSFI	CTRYAG	1 cut(s)	9
BstSLI	GKGCMC	1 cut(s)	236
BstUI	CGCG	4 cut(s)	54, 304, 426, 431
BstV1I	GCAGC	3 cut(s)	1246, 1268, 1361
BstV2I	GAAGAC	1 cut(s)	12
BstX2I	RGATCY	1 cut(s)	981
BstXI	CCANNNNNNTGG	1 cut(s)	507
BstYI	RGATCY	1 cut(s)	981
BstZI	CGGCCG	1 cut(s)	123
Bsu36I	CCTNAGG	1 cut(s)	603
BsuRI	GGCC	6 cut(s)	125, 191, 439, 461, 1011, 1188
BtsCI	GGATG	2 cut(s)	549, 805
BtsI	GCAGTG	1 cut(s)	530
BtsIMutI	CAGTG	4 cut(s)	530, 581, 801, 1023
Cac8I	GCNNGC	4 cut(s)	189, 1100, 1208, 1347
CaiI	CAGNNNCTG	1 cut(s)	929
CfoI	GCGC	5 cut(s)	56, 187, 264, 355, 729
Cfr10I	RCCGGY	2 cut(s)	152, 187
Cfr13I	GGNCC	1 cut(s)	1186
CseI	GACGC	2 cut(s)	415, 548
Csp6I	GTAC	3 cut(s)	535, 599, 719
CspAI	ACCGGT	1 cut(s)	152
CviAII	CATG	1 cut(s)	1445
CviQI	GTAC	3 cut(s)	535, 599, 719
DdeI	CTNAG	5 cut(s)	348, 603, 722, 1202, 1280
DpnI	GATC	4 cut(s)	411, 983, 1239, 1451
DpnII	GATC	4 cut(s)	409, 981, 1237, 1449
EaeI	YGGCCR	3 cut(s)	123, 189, 459
EagI	CGGCCG	1 cut(s)	123
Eam1104I	CTCTTC	5 cut(s)	63, 284, 315, 834, 891
EarI	CTCTTC	5 cut(s)	63, 284, 315, 834, 891
Ecl136II	GAGCTC	1 cut(s)	445
EclXI	CGGCCG	1 cut(s)	123
Eco130I	CCWWGG	1 cut(s)	228
Eco24I	GRGCYC	2 cut(s)	175, 447
Eco31I	GGTCTC	1 cut(s)	304
Eco52I	CGGCCG	1 cut(s)	123
Eco53kI	GAGCTC	1 cut(s)	445
Eco57I	CTGAAG	1 cut(s)	636
Eco81I	CCTNAGG	1 cut(s)	603
Eco88I	CYCGRG	1 cut(s)	341
EcoICRI	GAGCTC	1 cut(s)	445
EcoNI	CCTNNNNNAGG	1 cut(s)	206
EcoO109I	RGGNCCY	1 cut(s)	1186
EcoRII	CCWGG	3 cut(s)	236, 461, 1240
EcoT14I	CCWWGG	1 cut(s)	228
EcoT22I	ATGCAT	1 cut(s)	767
EcoT38I	GRGCYC	2 cut(s)	175, 447
ErhI	CCWWGG	1 cut(s)	228
FaeI	CATG	1 cut(s)	1448
FalI	AAGNNNNNCTT	4 cut(s)	609, 641, 888, 920
FaqI	GGGAC	2 cut(s)	254, 478
FatI	CATG	1 cut(s)	1444
FauI	CCCGC	1 cut(s)	255
FblI	GTMKAC	2 cut(s)	213, 327
Fnu4HI	GCNGC	6 cut(s)	126, 305, 557, 1257, 1260, 1350
FokI	GGATG	2 cut(s)	556, 812
FriOI	GRGCYC	2 cut(s)	175, 447
Fsp4HI	GCNGC	6 cut(s)	126, 305, 557, 1257, 1260, 1350
FspBI	CTAG	2 cut(s)	99, 1466
GlaI	GCGC	5 cut(s)	55, 186, 263, 354, 728
GluI	GCNGC	6 cut(s)	126, 305, 557, 1257, 1260, 1350
GsaI	CCCAGC	1 cut(s)	918
GsuI	CTGGAG	1 cut(s)	188
HaeII	RGCGCY	2 cut(s)	356, 730
HaeIII	GGCC	6 cut(s)	125, 191, 439, 461, 1011, 1188
HapII	CCGG	7 cut(s)	122, 153, 188, 335, 440, 1130, 1196
HgaI	GACGC	2 cut(s)	415, 548
HhaI	GCGC	5 cut(s)	56, 187, 264, 355, 729
Hin1I	GRCGYC	1 cut(s)	559
Hin1II	CATG	1 cut(s)	1448
Hin6I	GCGC	5 cut(s)	54, 185, 262, 353, 727
HinP1I	GCGC	5 cut(s)	54, 185, 262, 353, 727
HincII	GTYRAC	3 cut(s)	214, 220, 328
HindII	GTYRAC	3 cut(s)	214, 220, 328
HinfI	GANTC	4 cut(s)	382, 743, 790, 1388
HpaI	GTTAAC	1 cut(s)	220
HpaII	CCGG	7 cut(s)	122, 153, 188, 335, 440, 1130, 1196
HphI	GGTGA	7 cut(s)	79, 130, 481, 1130, 1163, 1423, 1448
Hpy166II	GTNNAC	5 cut(s)	214, 220, 328, 599, 1439
Hpy188I	TCNGA	6 cut(s)	365, 676, 838, 958, 976, 1393
Hpy188III	TCNNGA	6 cut(s)	205, 341, 343, 386, 563, 1235
Hpy8I	GTNNAC	5 cut(s)	214, 220, 328, 599, 1439
Hpy99I	CGWCG	4 cut(s)	218, 426, 431, 564
HpyAV	CCTTC	5 cut(s)	55, 202, 462, 1177, 1199
HpyCH4III	ACNGT	2 cut(s)	855, 1267
HpyCH4IV	ACGT	1 cut(s)	216
HpyCH4V	TGCA	7 cut(s)	11, 285, 765, 862, 1210, 1262, 1349
HpyF10VI	GCNNNNNNNGC	6 cut(s)	423, 529, 565, 762, 920, 1268
HpyF3I	CTNAG	5 cut(s)	348, 603, 722, 1202, 1280
HpySE526I	ACGT	1 cut(s)	216
Hsp92I	GRCGYC	1 cut(s)	559
Hsp92II	CATG	1 cut(s)	1448
HspAI	GCGC	5 cut(s)	54, 185, 262, 353, 727
KroI	GCCGGC	1 cut(s)	187
KroNI	GCCGGC	1 cut(s)	189
KspAI	GTTAAC	1 cut(s)	220
Kzo9I	GATC	4 cut(s)	409, 981, 1237, 1449
LguI	GCTCTTC	2 cut(s)	63, 284
LmnI	GCTCC	2 cut(s)	442, 565
Lsp1109I	GCAGC	3 cut(s)	1246, 1268, 1361
LweI	GCATC	6 cut(s)	174, 680, 774, 901, 982, 1144
MaeI	CTAG	2 cut(s)	99, 1466
MaeII	ACGT	1 cut(s)	216
MaeIII	GTNAC	4 cut(s)	386, 607, 640, 1062
MalI	GATC	4 cut(s)	411, 983, 1239, 1451
MboI	GATC	4 cut(s)	409, 981, 1237, 1449
MfeI	CAATTG	1 cut(s)	636
MflI	RGATCY	1 cut(s)	981
MhlI	GDGCHC	4 cut(s)	175, 236, 447, 1234
MlsI	TGGCCA	1 cut(s)	461
MluCI	AATT	6 cut(s)	286, 636, 663, 864, 962, 1169
MluNI	TGGCCA	1 cut(s)	461
MmeI	TCCRAC	1 cut(s)	661
Mox20I	TGGCCA	1 cut(s)	461
Mph1103I	ATGCAT	1 cut(s)	767
MroNI	GCCGGC	1 cut(s)	187
MroXI	GAANNNNTTC	1 cut(s)	900
MscI	TGGCCA	1 cut(s)	461
MseI	TTAA	5 cut(s)	219, 687, 1176, 1248, 1329
MslI	CAYNNNNRTG	1 cut(s)	505
Msp20I	TGGCCA	1 cut(s)	461
MspA1I	CMGCKG	2 cut(s)	394, 1259
MspCI	CTTAAG	1 cut(s)	1175
MspI	CCGG	7 cut(s)	122, 153, 188, 335, 440, 1130, 1196
MspR9I	CCNGG	4 cut(s)	122, 238, 463, 1242
MunI	CAATTG	1 cut(s)	636
MvaI	CCWGG	3 cut(s)	238, 463, 1242
MvnI	CGCG	4 cut(s)	54, 304, 426, 431
MwoI	GCNNNNNNNGC	6 cut(s)	423, 529, 565, 762, 920, 1268
NaeI	GCCGGC	1 cut(s)	189
NciI	CCSGG	1 cut(s)	122
NdeII	GATC	4 cut(s)	409, 981, 1237, 1449
NgoMIV	GCCGGC	1 cut(s)	187
NlaIII	CATG	1 cut(s)	1448
NlaIV	GGNNCC	2 cut(s)	235, 983
NmuCI	GTSAC	2 cut(s)	386, 640
NsiI	ATGCAT	1 cut(s)	767
NspV	TTCGAA	2 cut(s)	318, 405
PaeR7I	CTCGAG	1 cut(s)	341
PciSI	GCTCTTC	2 cut(s)	63, 284
PcsI	WCGNNNNNNNCGW	3 cut(s)	58, 324, 418
PdiI	GCCGGC	1 cut(s)	189
PdmI	GAANNNNTTC	1 cut(s)	900
PfeI	GAWTC	4 cut(s)	382, 743, 790, 1388
PfoI	TCCNGGA	1 cut(s)	1240
PinAI	ACCGGT	1 cut(s)	152
PkrI	GCNGC	6 cut(s)	127, 306, 558, 1258, 1261, 1351
Psp124BI	GAGCTC	1 cut(s)	447
Psp6I	CCWGG	3 cut(s)	236, 461, 1240
PspFI	CCCAGC	1 cut(s)	914
PspGI	CCWGG	3 cut(s)	236, 461, 1240
PspN4I	GGNNCC	2 cut(s)	235, 983
PspPI	GGNCC	1 cut(s)	1186
PstI	CTGCAG	1 cut(s)	13
PstNI	CAGNNNCTG	1 cut(s)	929
PsuI	RGATCY	1 cut(s)	981
PvuII	CAGCTG	2 cut(s)	394, 1259
RsaI	GTAC	3 cut(s)	536, 600, 720
RsaNI	GTAC	3 cut(s)	535, 599, 719
RseI	CAYNNNNRTG	1 cut(s)	505
SacI	GAGCTC	1 cut(s)	447
SalI	GTCGAC	2 cut(s)	212, 326
SapI	GCTCTTC	2 cut(s)	63, 284
SaqAI	TTAA	5 cut(s)	219, 687, 1176, 1248, 1329
SatI	GCNGC	6 cut(s)	126, 305, 557, 1257, 1260, 1350
Sau3AI	GATC	4 cut(s)	409, 981, 1237, 1449
Sau96I	GGNCC	1 cut(s)	1186
ScaI	AGTACT	1 cut(s)	720
ScrFI	CCNGG	4 cut(s)	122, 238, 463, 1242
SduI	GDGCHC	4 cut(s)	175, 236, 447, 1234
SfaNI	GCATC	6 cut(s)	174, 680, 774, 901, 982, 1144
SfcI	CTRYAG	1 cut(s)	9
Sfr274I	CTCGAG	1 cut(s)	341
SfuI	TTCGAA	2 cut(s)	318, 405
SgrAI	CRCCGGYG	1 cut(s)	152
SlaI	CTCGAG	1 cut(s)	341
SmiMI	CAYNNNNRTG	1 cut(s)	505
SmlI	CTYRAG	3 cut(s)	341, 650, 1175
SmoI	CTYRAG	3 cut(s)	341, 650, 1175
Sse9I	AATT	6 cut(s)	286, 636, 663, 864, 962, 1169
SsiI	CCGC	9 cut(s)	126, 159, 248, 268, 304, 532, 556, 1096, 1325
SspMI	CTAG	2 cut(s)	99, 1466
SstI	GAGCTC	1 cut(s)	447
StyD4I	CCNGG	4 cut(s)	120, 236, 461, 1240
StyI	CCWWGG	1 cut(s)	228
TaaI	ACNGT	2 cut(s)	855, 1267
TaiI	ACGT	1 cut(s)	219
TaqI	TCGA	8 cut(s)	61, 213, 318, 327, 342, 405, 412, 1038
TasI	AATT	6 cut(s)	286, 636, 663, 864, 962, 1169
TatI	WGTACW	1 cut(s)	718
TauI	GCSGC	3 cut(s)	128, 307, 559
TfiI	GAWTC	4 cut(s)	382, 743, 790, 1388
Tru1I	TTAA	5 cut(s)	219, 687, 1176, 1248, 1329
Tru9I	TTAA	5 cut(s)	219, 687, 1176, 1248, 1329
TscAI	CASTG	4 cut(s)	530, 581, 801, 1030
TseFI	GTSAC	2 cut(s)	386, 640
TseI	GCWGC	3 cut(s)	1256, 1259, 1349
Tsp45I	GTSAC	2 cut(s)	386, 640
TspGWI	ACGGA	1 cut(s)	1369
TspRI	CASTG	4 cut(s)	530, 581, 801, 1030
Vha464I	CTTAAG	1 cut(s)	1175
XagI	CCTNNNNNAGG	1 cut(s)	206
XapI	RAATTY	2 cut(s)	864, 962
XhoI	CTCGAG	1 cut(s)	341
XmiI	GTMKAC	2 cut(s)	213, 327
XmnI	GAANNNNTTC	1 cut(s)	900
XspI	CTAG	2 cut(s)	99, 1466
ZrmI	AGTACT	1 cut(s)	720
Zsp2I	ATGCAT	1 cut(s)	767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.