Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006089

Physical Location & Seq

Genomic Coordinates Reverse (-)

17862164 .. 17870364

Gene Structure 8201 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1112900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGCTCTCTGCAGTCTCGTCTTCCCTCCTTACCTTCCACAAGCCCTTCACTCGCGCTCTTCGACTCCCCCTCCCCAAACTCCCCATTTCTTCAAACCCTAAACCCCTCTCTTCTCTCTCCCCCGCCACTTCTTCTCTCATCCCCAATTCCACCGGCGGTGGTCGCGCCGGGGCTTTATCTCCGGCCCCGGCCACCGACGACCTCCAGAAGATCGATGTTAACCCTCCCAAGGGCACGAGGGACTTCCCACCCGAAGATATGCGCCTCCGCAACTGGCTCTTCTACAATTTCAGAGAGGTCTCGCGGCTGTTCGGCTTCGAAGAGGTCGACTATCCGGTTCTTGAGACGGAGGCTCTGTTTATCAGAAAGGCAGGGGAGGAGATTCGAGACCAGCTGTATTGCTTCGAAGATCGGGGAAATCGTCGCGTTGCGCTGAGGCCGGAGCTCACTCCTTCTTTGGCAAGGCTGGTGATACAGAAAGGAAAATCTCTATCCCTTCCGTTGAAATGGTTTGCCATTGGGCAGTGTTGGCGGTATGAGAGGATGACAAGGGGGCGGCGTCGGGAGCATTACCAGTGGAATATGGATGTTATTGGTGTACCTGAGGTTACGGCTGAAGCAGAACTTATTTCTTCAATTGTCACTTTCTTCAAGCGAGTAGGAATTACAGCATCAGATGTTGGATTCAAGGTTTCTAGTCGTAAGGTTTTACAAGAAGTATTGAGGCGTTATTCCATACCCGAAGCTTTATTTGGCAAAGTTTGCATCATCATAGACAAGATAGAGAAGATTCCAGTGGATGATATAAAAAATGAGTTGAAATCTGCTGGTGTATCTGAAGAGGCTATTGAACAGTTATTGCAAGTTCTTTCCATAAAGTCATTGACAAAGCTGGAAGAGATACTTGGAGGAGCAGGGGAAGCAGTTGCTGATCTGAAACAACTATTCTCTCTTGCTGACAAATTTGGTTACTCTGAATGGATTCAGTTTGATGCTTCTATTGTTCGTGGCCTTGCCTACTACACTGGTATTGTCTTTGAGGGTTTTGATAGAGGAGGAAAGTTACGAGCCATTTGTGGTGGTGGGCGATATGACCGCTTGCTTTCTACTTTTGGTGGTGATGACATTTCTGCTTGTGGATTTGGGTTTGGTGATGCTGTTATAGTGGAATTGCTTAAGGAGAAGGGTCTTCTACCGGAACTCAGCCTGCAAGTAGAGAACATTGTGTGTGCTCTTGATCCTGGACTCCAAGGAGCAGCTGCAACAGTTGCCACCATACTCAGGGGAAAAGGTCAAAGTGTTGATTTAGTCTTGGAGAACAAACCGCTTAAATGGGTATTCAAGCGTGCATCACGAATAAATGCTAACAGGCTGATATTGGTGGGAGATTCGGAGTGGCAAAGGGGTATGGTGAGTGTGAAAGTCCTTTCTTCCGGCGAACAACGTGAGATCAAACTTGATGAACTAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004821 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006427 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_His

Protein Analysis

491

Amino Acids

54.38

Weight (kDa)

8.56

Isoelectric Point (pI)

41.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_His	PF13393	78 - 382	2.8e-42	Histidyl-tRNA synthetase
tRNA-synt_2b	PF00587	133 - 229	8.6e-11	tRNA synthetase class II core domain (G, H, P, S and T)
HGTP_anticodon	PF03129	411 - 489	2e-09	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46100 AT5G03406
fragaria_vesca	FvH4_3g29130 FvH4_3g29130 FvH4_3g29130
malus_domestica	MD04G1093200.v1.1 MD12G1112900.v1.1
prunus_persica	Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1
pyrus_communis	pycom04g08820
rosa_chinensis	RchiOBHm_Chr3g0470291
rosa_laevigata	RLG00000024282
rosa_multiflora	Rmu_sc0011073.1_g000001 Rmu_sc0019034.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410990
rosa_rugosa	Rorug03G0107800 Rorug03G0107900
rosa_samantha	Rh3AG157600 Rh3BG181800 Rh3CG172500 Rh3DG193100
rosa_wichuraiana	Rw3G014990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	327
AccII	CGCG	4 cut(s)	54, 165, 304, 426
AciI	CCGC	8 cut(s)	123, 156, 268, 304, 532, 556, 1096, 1325
AclWI	GGATC	1 cut(s)	1232
AcoI	YGGCCR	1 cut(s)	189
AcsI	RAATTY	1 cut(s)	962
AcuI	CTGAAG	2 cut(s)	636, 858
AcyI	GRCGYC	1 cut(s)	559
AfaI	GTAC	1 cut(s)	600
AfiI	CCNNNNNNNGG	3 cut(s)	229, 230, 1281
AflII	CTTAAG	1 cut(s)	1175
AgsI	TTSAA	8 cut(s)	93, 505, 636, 652, 688, 820, 851, 1342
AjnI	CCWGG	1 cut(s)	1240
AjuI	GAANNNNNNNTTGG	4 cut(s)	735, 767, 888, 920
AluBI	AGCT	5 cut(s)	394, 445, 746, 892, 1259
AluI	AGCT	5 cut(s)	394, 445, 746, 892, 1259
Alw21I	GWGCWC	2 cut(s)	447, 1234
Alw26I	GTCTC	4 cut(s)	19, 304, 338, 381
AlwI	GGATC	1 cut(s)	1232
AlwNI	CAGNNNCTG	1 cut(s)	929
AoxI	GGCC	4 cut(s)	183, 189, 437, 1009
ApeKI	GCWGC	2 cut(s)	1256, 1259
ApoI	RAATTY	1 cut(s)	962
Asp700I	GAANNNNTTC	1 cut(s)	981
AspLEI	GCGC	4 cut(s)	56, 167, 264, 433
AspS9I	GGNCC	1 cut(s)	184
AsuC2I	CCSGG	2 cut(s)	169, 188
AsuHPI	GGTGA	4 cut(s)	481, 1130, 1163, 1423
AsuII	TTCGAA	2 cut(s)	318, 405
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	1 cut(s)	236
BanII	GRGCYC	1 cut(s)	447
BauI	CACGAG	1 cut(s)	235
BbsI	GAAGAC	2 cut(s)	12, 1181
Bbv12I	GWGCWC	2 cut(s)	447, 1234
BbvCI	CCTCAGC	1 cut(s)	434
BbvI	GCAGC	2 cut(s)	1246, 1268
BceAI	ACGGC	1 cut(s)	627
BciT130I	CCWGG	1 cut(s)	1242
BcnI	CCSGG	2 cut(s)	169, 188
BcoDI	GTCTC	4 cut(s)	19, 304, 338, 381
BfaI	CTAG	2 cut(s)	696, 1466
BfmI	CTRYAG	1 cut(s)	9
BfrI	CTTAAG	1 cut(s)	1175
BisI	GCNGC	4 cut(s)	305, 557, 1257, 1260
BlsI	GCNGC	4 cut(s)	306, 558, 1258, 1261
Bme1390I	CCNGG	3 cut(s)	169, 188, 1242
BmgT120I	GGNCC	1 cut(s)	184
BmiI	GGNNCC	1 cut(s)	186
BmrFI	CCNGG	3 cut(s)	169, 188, 1242
BmsI	GCATC	5 cut(s)	680, 774, 982, 1144, 1358
BpiI	GAAGAC	2 cut(s)	12, 1181
BpmI	CTGGAG	1 cut(s)	188
Bpu10I	CCTNAGC	1 cut(s)	434
Bpu14I	TTCGAA	2 cut(s)	318, 405
BpuEI	CTTGAG	1 cut(s)	362
BpuMI	CCSGG	2 cut(s)	169, 188
Bsa29I	ATCGAT	1 cut(s)	213
BsaHI	GRCGYC	1 cut(s)	559
BsaI	GGTCTC	2 cut(s)	304, 381
BsaJI	CCNNGG	4 cut(s)	168, 186, 228, 1249
BsaWI	WCCGGW	2 cut(s)	334, 1195
BsaXI	ACNNNNNCTCC	4 cut(s)	54, 84, 341, 371
Bsc4I	CCNNNNNNNGG	3 cut(s)	229, 230, 1281
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	4 cut(s)	278, 574, 794, 1030
Bse21I	CCTNAGG	1 cut(s)	603
BseBI	CCWGG	1 cut(s)	1242
BseCI	ATCGAT	1 cut(s)	213
BseDI	CCNNGG	4 cut(s)	168, 186, 228, 1249
BseGI	GGATG	4 cut(s)	138, 549, 592, 805
BseLI	CCNNNNNNNGG	3 cut(s)	229, 230, 1281
BseMII	CTCAG	4 cut(s)	425, 594, 1216, 1294
BseNI	ACTGG	4 cut(s)	278, 574, 794, 1030
BseRI	GAGGAG	3 cut(s)	392, 924, 1068
BseSI	GKGCMC	1 cut(s)	236
BseXI	GCAGC	2 cut(s)	1246, 1268
Bsh1236I	CGCG	4 cut(s)	54, 165, 304, 426
BshFI	GGCC	4 cut(s)	185, 191, 439, 1011
BshVI	ATCGAT	1 cut(s)	213
BsiHKAI	GWGCWC	2 cut(s)	447, 1234
BsiSI	CCGG	8 cut(s)	153, 168, 182, 188, 335, 440, 1196, 1434
BslFI	GGGAC	1 cut(s)	254
BslI	CCNNNNNNNGG	3 cut(s)	229, 230, 1281
BsmAI	GTCTC	4 cut(s)	19, 304, 338, 381
BsmBI	CGTCTC	1 cut(s)	338
BsmFI	GGGAC	1 cut(s)	254
BsnI	GGCC	4 cut(s)	185, 191, 439, 1011
Bso31I	GGTCTC	2 cut(s)	304, 381
Bsp119I	TTCGAA	2 cut(s)	318, 405
Bsp1286I	GDGCHC	3 cut(s)	236, 447, 1234
Bsp143I	GATC	5 cut(s)	210, 409, 931, 1237, 1449
BspACI	CCGC	8 cut(s)	123, 156, 268, 304, 532, 556, 1096, 1325
BspANI	GGCC	4 cut(s)	185, 191, 439, 1011
BspCNI	CTCAG	4 cut(s)	426, 595, 1215, 1293
BspDI	ATCGAT	1 cut(s)	213
BspFNI	CGCG	4 cut(s)	54, 165, 304, 426
BspLI	GGNNCC	1 cut(s)	186
BspMAI	CTGCAG	1 cut(s)	13
BspPI	GGATC	1 cut(s)	1232
BspQI	GCTCTTC	2 cut(s)	63, 284
BspT104I	TTCGAA	2 cut(s)	318, 405
BspTI	CTTAAG	1 cut(s)	1175
BspTNI	GGTCTC	2 cut(s)	304, 381
BsrFI	RCCGGY	1 cut(s)	152
BsrI	ACTGG	4 cut(s)	278, 574, 794, 1030
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	4 cut(s)	168, 186, 228, 1249
BssMI	GATC	5 cut(s)	210, 409, 931, 1237, 1449
BssNI	GRCGYC	1 cut(s)	559
BssSI	CACGAG	1 cut(s)	235
BssT1I	CCWWGG	2 cut(s)	228, 1249
Bst2BI	CACGAG	1 cut(s)	235
Bst2UI	CCWGG	1 cut(s)	1242
Bst4CI	ACNGT	2 cut(s)	855, 1267
Bst6I	CTCTTC	6 cut(s)	63, 115, 284, 315, 834, 891
BstACI	GRCGYC	1 cut(s)	559
BstAFI	CTTAAG	1 cut(s)	1175
BstAPI	GCANNNNNTGC	2 cut(s)	762, 1268
BstBI	TTCGAA	2 cut(s)	318, 405
BstC8I	GCNNGC	3 cut(s)	1100, 1208, 1347
BstDEI	CTNAG	4 cut(s)	434, 603, 1202, 1280
BstF5I	GGATG	4 cut(s)	138, 549, 592, 805
BstFNI	CGCG	4 cut(s)	54, 165, 304, 426
BstHHI	GCGC	4 cut(s)	56, 167, 264, 433
BstKTI	GATC	5 cut(s)	213, 412, 934, 1240, 1452
BstMAI	GTCTC	4 cut(s)	19, 304, 338, 381
BstMBI	GATC	5 cut(s)	210, 409, 931, 1237, 1449
BstMWI	GCNNNNNNNGC	6 cut(s)	162, 529, 565, 762, 920, 1268
BstNI	CCWGG	1 cut(s)	1242
BstSCI	CCNGG	3 cut(s)	167, 186, 1240
BstSFI	CTRYAG	1 cut(s)	9
BstSLI	GKGCMC	1 cut(s)	236
BstUI	CGCG	4 cut(s)	54, 165, 304, 426
BstV1I	GCAGC	2 cut(s)	1246, 1268
BstV2I	GAAGAC	2 cut(s)	12, 1181
Bsu15I	ATCGAT	1 cut(s)	213
Bsu36I	CCTNAGG	1 cut(s)	603
BsuRI	GGCC	4 cut(s)	185, 191, 439, 1011
BsuTUI	ATCGAT	1 cut(s)	213
BtsCI	GGATG	4 cut(s)	138, 549, 592, 805
BtsI	GCAGTG	1 cut(s)	530
BtsIMutI	CAGTG	4 cut(s)	530, 581, 801, 1023
Cac8I	GCNNGC	3 cut(s)	1100, 1208, 1347
CaiI	CAGNNNCTG	1 cut(s)	929
CfoI	GCGC	4 cut(s)	56, 167, 264, 433
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	1 cut(s)	184
ClaI	ATCGAT	1 cut(s)	213
CseI	GACGC	1 cut(s)	548
Csp6I	GTAC	1 cut(s)	599
CviQI	GTAC	1 cut(s)	599
DdeI	CTNAG	4 cut(s)	434, 603, 1202, 1280
DpnI	GATC	5 cut(s)	212, 411, 933, 1239, 1451
DpnII	GATC	5 cut(s)	210, 409, 931, 1237, 1449
EaeI	YGGCCR	1 cut(s)	189
Eam1104I	CTCTTC	6 cut(s)	63, 115, 284, 315, 834, 891
EarI	CTCTTC	6 cut(s)	63, 115, 284, 315, 834, 891
Ecl136II	GAGCTC	1 cut(s)	445
Eco130I	CCWWGG	2 cut(s)	228, 1249
Eco24I	GRGCYC	1 cut(s)	447
Eco31I	GGTCTC	2 cut(s)	304, 381
Eco53kI	GAGCTC	1 cut(s)	445
Eco57I	CTGAAG	2 cut(s)	636, 858
Eco81I	CCTNAGG	1 cut(s)	603
EcoICRI	GAGCTC	1 cut(s)	445
EcoRII	CCWGG	1 cut(s)	1240
EcoT14I	CCWWGG	2 cut(s)	228, 1249
EcoT38I	GRGCYC	1 cut(s)	447
ErhI	CCWWGG	2 cut(s)	228, 1249
Esp3I	CGTCTC	1 cut(s)	338
FalI	AAGNNNNNCTT	4 cut(s)	609, 641, 888, 920
FaqI	GGGAC	1 cut(s)	254
FauI	CCCGC	1 cut(s)	130
FblI	GTMKAC	1 cut(s)	327
Fnu4HI	GCNGC	4 cut(s)	305, 557, 1257, 1260
FokI	GGATG	4 cut(s)	125, 556, 599, 812
FriOI	GRGCYC	1 cut(s)	447
Fsp4HI	GCNGC	4 cut(s)	305, 557, 1257, 1260
FspBI	CTAG	2 cut(s)	696, 1466
GlaI	GCGC	4 cut(s)	55, 166, 263, 432
GluI	GCNGC	4 cut(s)	305, 557, 1257, 1260
GsuI	CTGGAG	1 cut(s)	188
HaeIII	GGCC	4 cut(s)	185, 191, 439, 1011
HapII	CCGG	8 cut(s)	153, 168, 182, 188, 335, 440, 1196, 1434
HgaI	GACGC	1 cut(s)	548
HhaI	GCGC	4 cut(s)	56, 167, 264, 433
Hin1I	GRCGYC	1 cut(s)	559
Hin6I	GCGC	4 cut(s)	54, 165, 262, 431
HinP1I	GCGC	4 cut(s)	54, 165, 262, 431
HincII	GTYRAC	2 cut(s)	220, 328
HindII	GTYRAC	2 cut(s)	220, 328
HindIII	AAGCTT	1 cut(s)	744
HinfI	GANTC	7 cut(s)	63, 382, 684, 790, 982, 1245, 1388
HpaI	GTTAAC	1 cut(s)	220
HpaII	CCGG	8 cut(s)	153, 168, 182, 188, 335, 440, 1196, 1434
HphI	GGTGA	4 cut(s)	481, 1130, 1163, 1423
Hpy166II	GTNNAC	3 cut(s)	220, 328, 599
Hpy188I	TCNGA	7 cut(s)	293, 365, 676, 838, 936, 976, 1393
Hpy188III	TCNNGA	6 cut(s)	205, 341, 386, 563, 1235, 1353
Hpy8I	GTNNAC	3 cut(s)	220, 328, 599
Hpy99I	CGWCG	3 cut(s)	200, 426, 564
HpyAV	CCTTC	5 cut(s)	43, 55, 462, 506, 1177
HpyCH4III	ACNGT	2 cut(s)	855, 1267
HpyCH4IV	ACGT	1 cut(s)	1444
HpyCH4V	TGCA	6 cut(s)	11, 765, 862, 1210, 1262, 1349
HpyF10VI	GCNNNNNNNGC	6 cut(s)	162, 529, 565, 762, 920, 1268
HpyF3I	CTNAG	4 cut(s)	434, 603, 1202, 1280
HpySE526I	ACGT	1 cut(s)	1444
Hsp92I	GRCGYC	1 cut(s)	559
HspAI	GCGC	4 cut(s)	54, 165, 262, 431
KspAI	GTTAAC	1 cut(s)	220
Kzo9I	GATC	5 cut(s)	210, 409, 931, 1237, 1449
LguI	GCTCTTC	2 cut(s)	63, 284
LmnI	GCTCC	4 cut(s)	442, 565, 911, 1253
Lsp1109I	GCAGC	2 cut(s)	1246, 1268
LweI	GCATC	5 cut(s)	680, 774, 982, 1144, 1358
MaeI	CTAG	2 cut(s)	696, 1466
MaeII	ACGT	1 cut(s)	1444
MaeIII	GTNAC	4 cut(s)	607, 640, 968, 1062
MalI	GATC	5 cut(s)	212, 411, 933, 1239, 1451
MboI	GATC	5 cut(s)	210, 409, 931, 1237, 1449
MfeI	CAATTG	1 cut(s)	636
MhlI	GDGCHC	3 cut(s)	236, 447, 1234
MluCI	AATT	6 cut(s)	145, 286, 636, 663, 962, 1169
MlyI	GAGTC	2 cut(s)	57, 1239
MmeI	TCCRAC	1 cut(s)	661
MroXI	GAANNNNTTC	1 cut(s)	981
MseI	TTAA	3 cut(s)	219, 1176, 1329
MspA1I	CMGCKG	2 cut(s)	394, 1259
MspCI	CTTAAG	1 cut(s)	1175
MspI	CCGG	8 cut(s)	153, 168, 182, 188, 335, 440, 1196, 1434
MspR9I	CCNGG	3 cut(s)	169, 188, 1242
MunI	CAATTG	1 cut(s)	636
MvaI	CCWGG	1 cut(s)	1242
MvnI	CGCG	4 cut(s)	54, 165, 304, 426
MwoI	GCNNNNNNNGC	6 cut(s)	162, 529, 565, 762, 920, 1268
NciI	CCSGG	2 cut(s)	169, 188
NdeII	GATC	5 cut(s)	210, 409, 931, 1237, 1449
NlaIV	GGNNCC	1 cut(s)	186
NmuCI	GTSAC	1 cut(s)	640
NspV	TTCGAA	2 cut(s)	318, 405
PciSI	GCTCTTC	2 cut(s)	63, 284
PcsI	WCGNNNNNNNCGW	3 cut(s)	58, 324, 418
PdmI	GAANNNNTTC	1 cut(s)	981
PfeI	GAWTC	5 cut(s)	382, 684, 790, 982, 1388
PfoI	TCCNGGA	1 cut(s)	1240
PkrI	GCNGC	4 cut(s)	306, 558, 1258, 1261
PleI	GAGTC	2 cut(s)	57, 1239
PpsI	GAGTC	2 cut(s)	57, 1239
Psp124BI	GAGCTC	1 cut(s)	447
Psp6I	CCWGG	1 cut(s)	1240
PspGI	CCWGG	1 cut(s)	1240
PspN4I	GGNNCC	1 cut(s)	186
PspPI	GGNCC	1 cut(s)	184
PstI	CTGCAG	1 cut(s)	13
PstNI	CAGNNNCTG	1 cut(s)	929
PvuII	CAGCTG	2 cut(s)	394, 1259
RsaI	GTAC	1 cut(s)	600
RsaNI	GTAC	1 cut(s)	599
SacI	GAGCTC	1 cut(s)	447
SalI	GTCGAC	1 cut(s)	326
SapI	GCTCTTC	2 cut(s)	63, 284
SaqAI	TTAA	3 cut(s)	219, 1176, 1329
SatI	GCNGC	4 cut(s)	305, 557, 1257, 1260
Sau3AI	GATC	5 cut(s)	210, 409, 931, 1237, 1449
Sau96I	GGNCC	1 cut(s)	184
SchI	GAGTC	2 cut(s)	57, 1239
ScrFI	CCNGG	3 cut(s)	169, 188, 1242
SduI	GDGCHC	3 cut(s)	236, 447, 1234
SfaNI	GCATC	5 cut(s)	680, 774, 982, 1144, 1358
SfcI	CTRYAG	1 cut(s)	9
SfuI	TTCGAA	2 cut(s)	318, 405
SgrAI	CRCCGGYG	1 cut(s)	152
SmlI	CTYRAG	2 cut(s)	341, 1175
SmoI	CTYRAG	2 cut(s)	341, 1175
Sse9I	AATT	6 cut(s)	145, 286, 636, 663, 962, 1169
SsiI	CCGC	8 cut(s)	123, 156, 268, 304, 532, 556, 1096, 1325
SspMI	CTAG	2 cut(s)	696, 1466
SstI	GAGCTC	1 cut(s)	447
StyD4I	CCNGG	3 cut(s)	167, 186, 1240
StyI	CCWWGG	2 cut(s)	228, 1249
TaaI	ACNGT	2 cut(s)	855, 1267
TaiI	ACGT	1 cut(s)	1447
TaqI	TCGA	6 cut(s)	61, 213, 318, 327, 385, 405
TasI	AATT	6 cut(s)	145, 286, 636, 663, 962, 1169
TauI	GCSGC	2 cut(s)	307, 559
TfiI	GAWTC	5 cut(s)	382, 684, 790, 982, 1388
Tru1I	TTAA	3 cut(s)	219, 1176, 1329
Tru9I	TTAA	3 cut(s)	219, 1176, 1329
TscAI	CASTG	4 cut(s)	530, 581, 801, 1030
TseFI	GTSAC	1 cut(s)	640
TseI	GCWGC	2 cut(s)	1256, 1259
Tsp45I	GTSAC	1 cut(s)	640
TspGWI	ACGGA	2 cut(s)	362, 489
TspRI	CASTG	4 cut(s)	530, 581, 801, 1030
Vha464I	CTTAAG	1 cut(s)	1175
XapI	RAATTY	1 cut(s)	962
XmiI	GTMKAC	1 cut(s)	327
XmnI	GAANNNNTTC	1 cut(s)	981
XspI	CTAG	2 cut(s)	696, 1466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.