Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0006089

Physical Location & Seq

Genomic Coordinates Reverse (-)

34057047 .. 34064485

Gene Structure 7439 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024282

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGCTCTCTACAATTTCTTCTTCCCTCCTTATCCTCAATAAGCCCTTAACCCGCGCCCTCAGATTCCCCATCCCCAACCTACCCCTCGCCACAAACCCTAGGGGCCTCTCTTCTCTATCCACCGCCGCTATAAGCAACAATGCCGGCGGCGGTCGTGCCGGGGCATTGTCTCCGGCTCCGGTCACGGAGGACCTGCAGAAGATCGACGTCAACCCTCCCAAGGGCACCAGGGACTTCCCCCCCGAGGACATGCGCCTCCGCAGCTGGCTCTTCTTCAATTTCAGAGAGGTTTCGCGGTTGTTTGGCTTCGAGGAGGTGGATTTTCCGGTGCTCGAGTCGGAGGCTCTGTTTGTTAGAAAAGCAGGGGAGGAGATTCGAGACCAGCTGTATTGTTTCGAAGACAGGGGAAATCGTCGTGTTGCGTTGAGGCCTGAGCTTACTCCTTCGTTGGCGAGGCTGGTGATACAGAAAGGAAAATCTCTATCCCTTCCATTAAAATGGTTTGCAGTGGGGCAGTGTTGGCGCTATGAGAGAATGACAAGGGGACGGCGTCGGGAGCATTACCAGTGGAATATGGATATTATTGGTGTACCTGAGGTTTCGGCTGAAGCGGAACTTATTTCTGCAATTGTCACTTTCTTCAAGCGATTAGGAATTACAGCTTCGGATGTTGGATTTAAGGTTTCTAGTCGAAAGGTTTTACAAGAAGTATTGAGACGTTACTCCATACCGGAAGCTTTGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAGAAGCTTCCATTGGTGGAAATCAAAAAAGAGTTAGAATCTGTTGGTGTATTGGAAGAAGCAATTGAGCAGTTATTGCAAGTTCTTTCCATAAAGTCATTGACAGAGTTGGAAGAGATACTTGGAGATTCTGGGGAAGCAGTTGCTGATCTGAAGCAGCTGTTCTCTCTAGTGGACAAATTTGGTTATTCTGAATGGATTCAGTTCGATGCTTCTATTGTTCGTGGCCTTGCCTACTATACTGGTATAGTCTTCGAGGGTTTTGATAGAGGAGGAAAGTTGCGAGCCATTTGTGGTGGTGGACGATATGACCGGTTGCTCTCTACTTTTGGCGGCGATGATATTCCTGCTTGTGGCTTTGGATTTGGTGATGCTGTCATAATAGAATTGCTCAAGGAGAAGGGTCTTCTGCCAGAACTTGGCCTGCAAGTAGAGAACATCGTATGTGCTCTGGATCCTGGACTTCAAGGAGCAGCTGCAACAGTTGCCGCCATACTTAGGGAGAAAGGTCAAAGTGTTGATTTAGTATTGGAGAGCAAACCACTCAAATGGGTATTCAAGCGTGCTTCACGAATAAATGCTAACAGGCTGATCTTGGTGGGAGATTTAGAGTGGCAAAAGGACTCTGATAATGGGTGCTGGAACGTAAATGGCAATTCCCTTTATGAGGTGGATTCTGGAACTAAGACACTTGCTGGTAATGTCCCAATCCCAATCCAAGCAAATTTAGCAATAAACCAGTCACTCGATCTGGTTGTGACCACATGCATTGGCTTTTGTCTGGCTTTCTTAGTCATGATTACAGGGCATGACGATTCAGGACCTGGAAAAGGGAAAGAAGCAGAGAAGCGAAGGGAAAAGGGTTTTGTGATGAAAGCTGTAGTTGTTCAGGAAAACAGAATGAACGCCAACCAGCACCATGTCCAACGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004821 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006427 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

HGTP_anticodon tRNA-synt_His

Protein Analysis

569

Amino Acids

62.89

Weight (kDa)

6.94

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_His	PF13393	75 - 379	4.1e-42	Histidyl-tRNA synthetase
tRNA-synt_2b	PF00587	130 - 231	2e-11	tRNA synthetase class II core domain (G, H, P, S and T)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46100 AT5G03406
fragaria_vesca	FvH4_3g29130 FvH4_3g29130 FvH4_3g29130
malus_domestica	MD04G1093200.v1.1 MD12G1112900.v1.1
prunus_persica	Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1 Prupe.6G232000_v2.0.a1
pyrus_communis	pycom04g08820
rosa_chinensis	RchiOBHm_Chr3g0470291
rosa_laevigata	RLG00000024282
rosa_multiflora	Rmu_sc0011073.1_g000001 Rmu_sc0019034.1_g000001
rosa_roxburghii	Rroxscaffold_6G00410990
rosa_rugosa	Rorug03G0107800 Rorug03G0107900
rosa_samantha	Rh3AG157600 Rh3BG181800 Rh3CG172500 Rh3DG193100
rosa_wichuraiana	Rw3G014990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	210
Acc36I	ACCTGC	1 cut(s)	201
AccB1I	GGYRCC	1 cut(s)	224
AccB7I	CCANNNNNTGG	1 cut(s)	1193
AccII	CGCG	2 cut(s)	54, 295
AclI	AACGTT	1 cut(s)	1702
AclWI	GGATC	2 cut(s)	1223, 1236
AcsI	RAATTY	2 cut(s)	953, 1498
AcuI	CTGAAG	2 cut(s)	627, 947
AcyI	GRCGYC	2 cut(s)	207, 550
AfaI	GTAC	1 cut(s)	591
AfiI	CCNNNNNNNGG	8 cut(s)	220, 221, 244, 1127, 1193, 1272, 1441, 1604
AgeI	ACCGGT	1 cut(s)	1086
AgsI	TTSAA	4 cut(s)	277, 643, 1241, 1333
AjnI	CCWGG	3 cut(s)	227, 1231, 1597
AjuI	GAANNNNNNNTTGG	6 cut(s)	726, 758, 770, 802, 879, 911
AluBI	AGCT	9 cut(s)	264, 385, 436, 662, 737, 781, 934, 1250, 1652
AluI	AGCT	9 cut(s)	264, 385, 436, 662, 737, 781, 934, 1250, 1652
Alw21I	GWGCWC	2 cut(s)	333, 1225
Alw26I	GTCTC	3 cut(s)	174, 372, 709
AlwI	GGATC	2 cut(s)	1223, 1236
AlwNI	CAGNNNCTG	2 cut(s)	920, 1598
Ama87I	CYCGRG	2 cut(s)	242, 332
AoxI	GGCC	4 cut(s)	103, 428, 1000, 1195
ApeKI	GCWGC	4 cut(s)	261, 931, 1247, 1250
ApoI	RAATTY	2 cut(s)	953, 1498
AsiGI	ACCGGT	1 cut(s)	1086
Asp700I	GAANNNNTTC	1 cut(s)	972
AspA2I	CCTAGG	1 cut(s)	98
AspLEI	GCGC	3 cut(s)	56, 255, 525
AspS9I	GGNCC	3 cut(s)	103, 190, 1595
AsuC2I	CCSGG	1 cut(s)	160
AsuHPI	GGTGA	2 cut(s)	472, 1154
AsuII	TTCGAA	1 cut(s)	396
AvaI	CYCGRG	2 cut(s)	242, 332
AvaII	GGWCC	2 cut(s)	190, 1595
AvrII	CCTAGG	1 cut(s)	98
AxyI	CCTNAGG	1 cut(s)	594
BaeGI	GKGCMC	1 cut(s)	227
BamHI	GGATCC	1 cut(s)	1228
BanI	GGYRCC	1 cut(s)	224
BbsI	GAAGAC	3 cut(s)	405, 1018, 1172
Bbv12I	GWGCWC	2 cut(s)	333, 1225
BbvI	GCAGC	4 cut(s)	273, 943, 1237, 1259
BccI	CCATC	1 cut(s)	77
BceAI	ACGGC	1 cut(s)	563
BciT130I	CCWGG	3 cut(s)	229, 1233, 1599
BcnI	CCSGG	1 cut(s)	160
BcoDI	GTCTC	3 cut(s)	174, 372, 709
BfaI	CTAG	3 cut(s)	99, 687, 944
BfmI	CTRYAG	2 cut(s)	194, 1653
BfoI	RGCGCY	1 cut(s)	526
BfuAI	ACCTGC	1 cut(s)	201
BisI	GCNGC	8 cut(s)	126, 148, 262, 932, 1108, 1248, 1251, 1263
BlnI	CCTAGG	1 cut(s)	98
BlsI	GCNGC	8 cut(s)	127, 149, 263, 933, 1109, 1249, 1252, 1264
Bme1390I	CCNGG	4 cut(s)	160, 229, 1233, 1599
Bme18I	GGWCC	2 cut(s)	190, 1595
BmeT110I	CYCGRG	2 cut(s)	242, 332
BmgT120I	GGNCC	3 cut(s)	103, 190, 1595
BmiI	GGNNCC	4 cut(s)	104, 177, 226, 1230
BmrFI	CCNGG	4 cut(s)	160, 229, 1233, 1599
BmsI	GCATC	3 cut(s)	765, 973, 1135
BpiI	GAAGAC	3 cut(s)	405, 1018, 1172
Bpu10I	CCTNAGC	1 cut(s)	432
Bpu14I	TTCGAA	1 cut(s)	396
BpuEI	CTTGAG	1 cut(s)	1151
BpuMI	CCSGG	1 cut(s)	160
BsaHI	GRCGYC	2 cut(s)	207, 550
BsaI	GGTCTC	1 cut(s)	372
BsaJI	CCNNGG	5 cut(s)	98, 159, 219, 228, 243
BsaWI	WCCGGW	4 cut(s)	178, 325, 730, 1086
BsaXI	ACNNNNNCTCC	2 cut(s)	332, 362
Bsc4I	CCNNNNNNNGG	8 cut(s)	220, 221, 244, 1127, 1193, 1272, 1441, 1604
Bse118I	RCCGGY	2 cut(s)	143, 1086
Bse1I	ACTGG	3 cut(s)	565, 1021, 1513
Bse21I	CCTNAGG	1 cut(s)	594
BseBI	CCWGG	3 cut(s)	229, 1233, 1599
BseDI	CCNNGG	5 cut(s)	98, 159, 219, 228, 243
BseGI	GGATG	2 cut(s)	69, 673
BseLI	CCNNNNNNNGG	8 cut(s)	220, 221, 244, 1127, 1193, 1272, 1441, 1604
BseMII	CTCAG	3 cut(s)	73, 423, 585
BseNI	ACTGG	3 cut(s)	565, 1021, 1513
BseRI	GAGGAG	3 cut(s)	326, 383, 1059
BseSI	GKGCMC	1 cut(s)	227
BseXI	GCAGC	4 cut(s)	273, 943, 1237, 1259
Bsh1236I	CGCG	2 cut(s)	54, 295
Bsh1285I	CGRYCG	1 cut(s)	154
BshFI	GGCC	4 cut(s)	105, 430, 1002, 1197
BshNI	GGYRCC	1 cut(s)	224
BshTI	ACCGGT	1 cut(s)	1086
BsiEI	CGRYCG	1 cut(s)	154
BsiHKAI	GWGCWC	2 cut(s)	333, 1225
BsiHKCI	CYCGRG	2 cut(s)	242, 332
BsiSI	CCGG	7 cut(s)	144, 159, 173, 179, 326, 731, 1087
BslFI	GGGAC	3 cut(s)	245, 558, 1463
BslI	CCNNNNNNNGG	8 cut(s)	220, 221, 244, 1127, 1193, 1272, 1441, 1604
BsmAI	GTCTC	3 cut(s)	174, 372, 709
BsmBI	CGTCTC	1 cut(s)	709
BsmFI	GGGAC	3 cut(s)	245, 558, 1463
BsnI	GGCC	4 cut(s)	105, 430, 1002, 1197
Bso31I	GGTCTC	1 cut(s)	372
BsoBI	CYCGRG	2 cut(s)	242, 332
Bsp119I	TTCGAA	1 cut(s)	396
Bsp1286I	GDGCHC	3 cut(s)	227, 333, 1225
Bsp143I	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
BspANI	GGCC	4 cut(s)	105, 430, 1002, 1197
BspCNI	CTCAG	3 cut(s)	72, 424, 586
BspFNI	CGCG	2 cut(s)	54, 295
BspHI	TCATGA	1 cut(s)	1569
BspLI	GGNNCC	4 cut(s)	104, 177, 226, 1230
BspMAI	CTGCAG	1 cut(s)	198
BspMI	ACCTGC	1 cut(s)	201
BspPI	GGATC	2 cut(s)	1223, 1236
BspQI	GCTCTTC	1 cut(s)	275
BspT104I	TTCGAA	1 cut(s)	396
BspT107I	GGYRCC	1 cut(s)	224
BspTNI	GGTCTC	1 cut(s)	372
BsrFI	RCCGGY	2 cut(s)	143, 1086
BsrI	ACTGG	3 cut(s)	565, 1021, 1513
BssAI	RCCGGY	2 cut(s)	143, 1086
BssECI	CCNNGG	5 cut(s)	98, 159, 219, 228, 243
BssMI	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
BssNI	GRCGYC	2 cut(s)	207, 550
BssT1I	CCWWGG	2 cut(s)	98, 219
Bst2UI	CCWGG	3 cut(s)	229, 1233, 1599
Bst4CI	ACNGT	1 cut(s)	1258
Bst6I	CTCTTC	3 cut(s)	115, 275, 882
BstACI	GRCGYC	2 cut(s)	207, 550
BstAPI	GCANNNNNTGC	3 cut(s)	753, 850, 1259
BstBI	TTCGAA	1 cut(s)	396
BstC8I	GCNNGC	5 cut(s)	145, 266, 1059, 1199, 1338
BstDEI	CTNAG	6 cut(s)	59, 432, 594, 1271, 1458, 1564
BstENI	CCTNNNNNAGG	2 cut(s)	1439, 1602
BstF5I	GGATG	2 cut(s)	69, 673
BstFNI	CGCG	2 cut(s)	54, 295
BstH2I	RGCGCY	1 cut(s)	526
BstHHI	GCGC	3 cut(s)	56, 255, 525
BstKTI	GATC	5 cut(s)	204, 925, 1231, 1368, 1525
BstMAI	GTCTC	3 cut(s)	174, 372, 709
BstMBI	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
BstMCI	CGRYCG	1 cut(s)	154
BstMWI	GCNNNNNNNGC	7 cut(s)	261, 520, 556, 753, 850, 1259, 1502
BstNI	CCWGG	3 cut(s)	229, 1233, 1599
BstNSI	RCATGY	2 cut(s)	253, 1542
BstSCI	CCNGG	4 cut(s)	158, 227, 1231, 1597
BstSFI	CTRYAG	2 cut(s)	194, 1653
BstSLI	GKGCMC	1 cut(s)	227
BstUI	CGCG	2 cut(s)	54, 295
BstV1I	GCAGC	4 cut(s)	273, 943, 1237, 1259
BstV2I	GAAGAC	3 cut(s)	405, 1018, 1172
BstX2I	RGATCY	1 cut(s)	1228
BstXI	CCANNNNNNTGG	2 cut(s)	498, 1323
BstYI	RGATCY	1 cut(s)	1228
Bsu36I	CCTNAGG	1 cut(s)	594
BsuRI	GGCC	4 cut(s)	105, 430, 1002, 1197
BtgZI	GCGATG	1 cut(s)	1125
BtsCI	GGATG	2 cut(s)	69, 673
BtsI	GCAGTG	2 cut(s)	513, 521
BtsIMutI	CAGTG	3 cut(s)	513, 521, 572
BveI	ACCTGC	1 cut(s)	201
Cac8I	GCNNGC	5 cut(s)	145, 266, 1059, 1199, 1338
CaiI	CAGNNNCTG	2 cut(s)	920, 1598
CciI	TCATGA	1 cut(s)	1569
CfoI	GCGC	3 cut(s)	56, 255, 525
Cfr10I	RCCGGY	2 cut(s)	143, 1086
Cfr13I	GGNCC	3 cut(s)	103, 190, 1595
CseI	GACGC	1 cut(s)	539
Csp6I	GTAC	1 cut(s)	590
CspAI	ACCGGT	1 cut(s)	1086
CspCI	CAANNNNNGTGG	2 cut(s)	1525, 1560
CviAII	CATG	5 cut(s)	250, 1539, 1570, 1583, 1694
CviQI	GTAC	1 cut(s)	590
DdeI	CTNAG	6 cut(s)	59, 432, 594, 1271, 1458, 1564
DpnI	GATC	5 cut(s)	203, 924, 1230, 1367, 1524
DpnII	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
Eam1104I	CTCTTC	3 cut(s)	115, 275, 882
EarI	CTCTTC	3 cut(s)	115, 275, 882
Eco130I	CCWWGG	2 cut(s)	98, 219
Eco147I	AGGCCT	1 cut(s)	430
Eco31I	GGTCTC	1 cut(s)	372
Eco47I	GGWCC	2 cut(s)	190, 1595
Eco57I	CTGAAG	2 cut(s)	627, 947
Eco81I	CCTNAGG	1 cut(s)	594
Eco88I	CYCGRG	2 cut(s)	242, 332
EcoNI	CCTNNNNNAGG	2 cut(s)	1439, 1602
EcoO109I	RGGNCCY	3 cut(s)	103, 190, 1595
EcoRII	CCWGG	3 cut(s)	227, 1231, 1597
EcoT14I	CCWWGG	2 cut(s)	98, 219
EcoT22I	ATGCAT	1 cut(s)	1544
ErhI	CCWWGG	2 cut(s)	98, 219
Esp3I	CGTCTC	1 cut(s)	709
FaeI	CATG	5 cut(s)	253, 1542, 1573, 1586, 1697
FalI	AAGNNNNNCTT	4 cut(s)	600, 632, 879, 911
FaqI	GGGAC	3 cut(s)	245, 558, 1463
FatI	CATG	5 cut(s)	249, 1538, 1569, 1582, 1693
FauI	CCCGC	1 cut(s)	59
Fnu4HI	GCNGC	8 cut(s)	126, 148, 262, 932, 1108, 1248, 1251, 1263
FokI	GGATG	2 cut(s)	56, 680
Fsp4HI	GCNGC	8 cut(s)	126, 148, 262, 932, 1108, 1248, 1251, 1263
FspBI	CTAG	3 cut(s)	99, 687, 944
GlaI	GCGC	3 cut(s)	55, 254, 524
GluI	GCNGC	8 cut(s)	126, 148, 262, 932, 1108, 1248, 1251, 1263
HaeII	RGCGCY	1 cut(s)	526
HaeIII	GGCC	4 cut(s)	105, 430, 1002, 1197
HapII	CCGG	7 cut(s)	144, 159, 173, 179, 326, 731, 1087
HgaI	GACGC	1 cut(s)	539
HhaI	GCGC	3 cut(s)	56, 255, 525
Hin1I	GRCGYC	2 cut(s)	207, 550
Hin1II	CATG	5 cut(s)	253, 1542, 1573, 1586, 1697
Hin6I	GCGC	3 cut(s)	54, 253, 523
HinP1I	GCGC	3 cut(s)	54, 253, 523
HincII	GTYRAC	1 cut(s)	211
HindII	GTYRAC	1 cut(s)	211
HindIII	AAGCTT	2 cut(s)	735, 779
HinfI	GANTC	9 cut(s)	63, 335, 373, 812, 902, 973, 1397, 1448, 1589
HpaII	CCGG	7 cut(s)	144, 159, 173, 179, 326, 731, 1087
HphI	GGTGA	2 cut(s)	472, 1154
Hpy166II	GTNNAC	4 cut(s)	211, 590, 949, 1076
Hpy188I	TCNGA	7 cut(s)	62, 284, 340, 667, 927, 967, 1402
Hpy188III	TCNNGA	8 cut(s)	377, 554, 1226, 1344, 1452, 1570, 1593, 1664
Hpy8I	GTNNAC	4 cut(s)	211, 590, 949, 1076
Hpy99I	CGWCG	3 cut(s)	209, 417, 555
HpyAV	CCTTC	4 cut(s)	453, 497, 1168, 1620
HpyCH4III	ACNGT	1 cut(s)	1258
HpyCH4IV	ACGT	4 cut(s)	207, 718, 1419, 1702
HpyCH4V	TGCA	8 cut(s)	196, 506, 626, 756, 853, 1201, 1253, 1542
HpyF10VI	GCNNNNNNNGC	7 cut(s)	261, 520, 556, 753, 850, 1259, 1502
HpyF3I	CTNAG	6 cut(s)	59, 432, 594, 1271, 1458, 1564
HpySE526I	ACGT	4 cut(s)	207, 718, 1419, 1702
Hsp92I	GRCGYC	2 cut(s)	207, 550
Hsp92II	CATG	5 cut(s)	253, 1542, 1573, 1586, 1697
HspAI	GCGC	3 cut(s)	54, 253, 523
KroI	GCCGGC	1 cut(s)	143
KroNI	GCCGGC	1 cut(s)	145
Kzo9I	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
LguI	GCTCTTC	1 cut(s)	275
LmnI	GCTCC	3 cut(s)	181, 556, 1244
Lsp1109I	GCAGC	4 cut(s)	273, 943, 1237, 1259
LweI	GCATC	3 cut(s)	765, 973, 1135
MaeI	CTAG	3 cut(s)	99, 687, 944
MaeII	ACGT	4 cut(s)	207, 718, 1419, 1702
MaeIII	GTNAC	5 cut(s)	181, 631, 719, 1515, 1531
MalI	GATC	5 cut(s)	203, 924, 1230, 1367, 1524
MboI	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
MfeI	CAATTG	2 cut(s)	627, 837
MflI	RGATCY	1 cut(s)	1228
MhlI	GDGCHC	3 cut(s)	227, 333, 1225
MluCI	AATT	9 cut(s)	12, 277, 627, 654, 837, 953, 1160, 1429, 1498
MlyI	GAGTC	2 cut(s)	344, 1391
MmeI	TCCRAC	3 cut(s)	318, 652, 864
Mph1103I	ATGCAT	1 cut(s)	1544
MroNI	GCCGGC	1 cut(s)	143
MroXI	GAANNNNTTC	1 cut(s)	972
MseI	TTAA	3 cut(s)	47, 494, 678
MslI	CAYNNNNRTG	2 cut(s)	496, 1321
MspA1I	CMGCKG	4 cut(s)	264, 385, 934, 1250
MspI	CCGG	7 cut(s)	144, 159, 173, 179, 326, 731, 1087
MspR9I	CCNGG	4 cut(s)	160, 229, 1233, 1599
MunI	CAATTG	2 cut(s)	627, 837
MvaI	CCWGG	3 cut(s)	229, 1233, 1599
MvnI	CGCG	2 cut(s)	54, 295
MwoI	GCNNNNNNNGC	7 cut(s)	261, 520, 556, 753, 850, 1259, 1502
NaeI	GCCGGC	1 cut(s)	145
NciI	CCSGG	1 cut(s)	160
NdeII	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
NgoMIV	GCCGGC	1 cut(s)	143
NlaIII	CATG	5 cut(s)	253, 1542, 1573, 1586, 1697
NlaIV	GGNNCC	4 cut(s)	104, 177, 226, 1230
NmuCI	GTSAC	4 cut(s)	181, 631, 1515, 1531
NsiI	ATGCAT	1 cut(s)	1544
NspI	RCATGY	2 cut(s)	253, 1542
NspV	TTCGAA	1 cut(s)	396
PaeR7I	CTCGAG	1 cut(s)	332
PagI	TCATGA	1 cut(s)	1569
PceI	AGGCCT	1 cut(s)	430
PciSI	GCTCTTC	1 cut(s)	275
PdiI	GCCGGC	1 cut(s)	145
PdmI	GAANNNNTTC	1 cut(s)	972
PfeI	GAWTC	7 cut(s)	63, 373, 812, 902, 973, 1448, 1589
PflFI	GACNNNGTC	1 cut(s)	549
PflMI	CCANNNNNTGG	1 cut(s)	1193
PfoI	TCCNGGA	1 cut(s)	1231
PinAI	ACCGGT	1 cut(s)	1086
PkrI	GCNGC	8 cut(s)	127, 149, 263, 933, 1109, 1249, 1252, 1264
PleI	GAGTC	2 cut(s)	343, 1391
PpsI	GAGTC	2 cut(s)	343, 1391
PpuMI	RGGWCCY	2 cut(s)	190, 1595
Psp1406I	AACGTT	1 cut(s)	1702
Psp5II	RGGWCCY	2 cut(s)	190, 1595
Psp6I	CCWGG	3 cut(s)	227, 1231, 1597
PspGI	CCWGG	3 cut(s)	227, 1231, 1597
PspN4I	GGNNCC	4 cut(s)	104, 177, 226, 1230
PspPI	GGNCC	3 cut(s)	103, 190, 1595
PspPPI	RGGWCCY	2 cut(s)	190, 1595
PspXI	VCTCGAGB	1 cut(s)	332
PstI	CTGCAG	1 cut(s)	198
PstNI	CAGNNNCTG	2 cut(s)	920, 1598
PsuI	RGATCY	1 cut(s)	1228
PsyI	GACNNNGTC	1 cut(s)	549
PvuII	CAGCTG	4 cut(s)	264, 385, 934, 1250
RsaI	GTAC	1 cut(s)	591
RsaNI	GTAC	1 cut(s)	590
RseI	CAYNNNNRTG	2 cut(s)	496, 1321
SapI	GCTCTTC	1 cut(s)	275
SaqAI	TTAA	3 cut(s)	47, 494, 678
SatI	GCNGC	8 cut(s)	126, 148, 262, 932, 1108, 1248, 1251, 1263
Sau3AI	GATC	5 cut(s)	201, 922, 1228, 1365, 1522
Sau96I	GGNCC	3 cut(s)	103, 190, 1595
SchI	GAGTC	2 cut(s)	344, 1391
ScrFI	CCNGG	4 cut(s)	160, 229, 1233, 1599
SduI	GDGCHC	3 cut(s)	227, 333, 1225
SfaNI	GCATC	3 cut(s)	765, 973, 1135
SfcI	CTRYAG	2 cut(s)	194, 1653
Sfr274I	CTCGAG	1 cut(s)	332
SfuI	TTCGAA	1 cut(s)	396
SinI	GGWCC	2 cut(s)	190, 1595
SlaI	CTCGAG	1 cut(s)	332
SmiMI	CAYNNNNRTG	2 cut(s)	496, 1321
SmlI	CTYRAG	2 cut(s)	332, 1166
SmoI	CTYRAG	2 cut(s)	332, 1166
Sse9I	AATT	9 cut(s)	12, 277, 627, 654, 837, 953, 1160, 1429, 1498
SseBI	AGGCCT	1 cut(s)	430
SspMI	CTAG	3 cut(s)	99, 687, 944
StuI	AGGCCT	1 cut(s)	430
StyD4I	CCNGG	4 cut(s)	158, 227, 1231, 1597
StyI	CCWWGG	2 cut(s)	98, 219
TaaI	ACNGT	1 cut(s)	1258
TaiI	ACGT	4 cut(s)	210, 721, 1422, 1705
TaqI	TCGA	9 cut(s)	204, 309, 333, 376, 396, 691, 981, 1029, 1521
TasI	AATT	9 cut(s)	12, 277, 627, 654, 837, 953, 1160, 1429, 1498
TauI	GCSGC	4 cut(s)	128, 150, 1110, 1265
TfiI	GAWTC	7 cut(s)	63, 373, 812, 902, 973, 1448, 1589
Tru1I	TTAA	3 cut(s)	47, 494, 678
Tru9I	TTAA	3 cut(s)	47, 494, 678
TscAI	CASTG	3 cut(s)	513, 521, 572
TseFI	GTSAC	4 cut(s)	181, 631, 1515, 1531
TseI	GCWGC	4 cut(s)	261, 931, 1247, 1250
Tsp45I	GTSAC	4 cut(s)	181, 631, 1515, 1531
TspDTI	ATGAA	2 cut(s)	1661, 1691
TspGWI	ACGGA	1 cut(s)	200
TspRI	CASTG	3 cut(s)	513, 521, 572
Tth111I	GACNNNGTC	1 cut(s)	549
Van91I	CCANNNNNTGG	1 cut(s)	1193
VpaK11BI	GGWCC	2 cut(s)	190, 1595
XagI	CCTNNNNNAGG	2 cut(s)	1439, 1602
XapI	RAATTY	2 cut(s)	953, 1498
XceI	RCATGY	2 cut(s)	253, 1542
XhoI	CTCGAG	1 cut(s)	332
XmaJI	CCTAGG	1 cut(s)	98
XmnI	GAANNNNTTC	1 cut(s)	972
XspI	CTAG	3 cut(s)	99, 687, 944
ZraI	GACGTC	1 cut(s)	208
Zsp2I	ATGCAT	1 cut(s)	1544

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.