AT5G03770

3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008414
Physical Location & Seq
Forward (+)
995095 .. 997849
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03770.3

Sequence Viewer

Length: 1344 bp
ATGAAGCTCGGAGTGTTTGTATACAGATTATACAGAGCTTTAACCTACGGTGTCTCCCCGTTGATTCACCTACACATACGGTGGCGCAGACTCCGGGGATTAGAGCATTTCAGCCGTTGGCCCGAGCGGTTCGGTCATCCCTCCGCCGTAAGACCACCGGGATCGCTTATCTGGTTTCACGCCGTCTCTTTAGGTGAAGGAATGGCTGCTATTCCGGTGATCAGACACTGCAATGAAGTAAAGTCAGATTTGACTATCCTAATGACGACGACGACAGTATCTGCATTTGAAGTAATAAAGAATCAGCTCCCTGTTGGTGTATTACATCAGTTTGCACCACTGGATACACCTTTAGCTATTGACAGATTCCTTGGCCATTGGAAACCGAATGCGATTATCATAATGGAGAACGAACTCTGGCCTAATCTTATCATGGCTGCTTCAGGACTTCTAATTCCGTTGGGATTGTTGAACGCTCGGATGTCTACTAAATCCTTTAAACGGTGGTCGAGTCCGCTCTTACTACCGCTGGTATCGTTGTTGCTATCTAAATTCTCATTGATAGCCCCACTGAGCACCCTGCAGGGTATACGTTTTCAGTTGCTGCATGCGCCACCCTTTGTCATAAATTATTCTGGTGACTTAAAATACGTGGTAAACAAGTTCCATGTATCTAGTGGAACATCTGAAAGTATAAGAGATCTAAAAGTAGAACTCGCAGAAATGAAAGTCTGGATTGCATCTTCATTACATAGAGGCGAAGAAGAAGTCATTTTAGGAGTTCACAACATGCTTCTCGAATCACATCCGGACTCTGTTGTCATAATTGTGCCTCGACATCCACATCATGGCCAACAGATTGCTCATAAACTGCGGAAAGATGGCCAAAGTGTTGCTCTAAGGTCTCAAAACGAAAAGCTTACACCAAGGAAGACGAATATATATGTGGTGGATACACTAGGTGAACTAAGGGAATTGTACAGTGTCGCTCCAATAGCCGTCATTGGAGGCTCTTTCATCCCGGGCTTAACTGGTCACAACTTGTCTGAAGCAGCTGCTGCTGGCTGCGCCGTTATTACAGGTTGTCATGTTGGGCATTTCTCTCACATGGTGAAGGCGATGCAGCAGGCGAACCCCTTATCGGTGACACAGGTCTCGACAAAACTAGAGCTCAAAGAAGCTGTCGACTTGCTCCTGAGTAACCCTGAGATCTTGGAAACACATCAGAGAGCTTCTAAAGACGTTTATGAATCTCTATCAAGCTGCATTATCACTAACATCTGGAAACTTCTCAATCTTCATATCTTTAGAGGGAAATCAAGAAATCACATTGAATGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00540 ko01100 map00540 map01100
Pfam Domains
Protein Families
Glycos_transf_N

Protein Analysis

447

Amino Acids

49.89

Weight (kDa)

9.5

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_N PF04413 2 - 150 2.6e-40 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 818
AccB7I CCANNNNNTGG 1 cut(s) 848
AccBSI CCGCTC 2 cut(s) 127, 517
AccI GTMKAC 4 cut(s) 21, 485, 589, 1185
AccIII TCCGGA 1 cut(s) 808
AciI CCGC 5 cut(s) 127, 144, 515, 527, 874
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 3 cut(s) 373, 850, 883
AcsI RAATTY 1 cut(s) 551
AcuI CTGAAG 2 cut(s) 426, 1068
AdeI CACNNNGTG 2 cut(s) 962, 1111
AfaI GTAC 1 cut(s) 980
AfiI CCNNNNNNNGG 3 cut(s) 501, 848, 1141
AgsI TTSAA 3 cut(s) 290, 472, 1334
Alw21I GWGCWC 2 cut(s) 578, 1173
Alw26I GTCTC 4 cut(s) 58, 190, 909, 1159
AlwI GGATC 1 cut(s) 169
AlwNI CAGNNNCTG 4 cut(s) 228, 281, 604, 1058
Ama87I CYCGRG 2 cut(s) 122, 1021
Aor13HI TCCGGA 1 cut(s) 808
AoxI GGCC 5 cut(s) 119, 373, 419, 850, 883
ApeKI GCWGC 9 cut(s) 206, 437, 604, 1052, 1055, 1058, 1065, 1123, 1263
ApoI RAATTY 1 cut(s) 551
ArsI GACNNNNNNTTYG 4 cut(s) 753, 785, 1167, 1199
AspLEI GCGC 3 cut(s) 87, 613, 1070
AspS9I GGNCC 1 cut(s) 120
AsuC2I CCSGG 4 cut(s) 95, 159, 1022, 1023
AsuHPI GGTGA 7 cut(s) 59, 206, 229, 650, 974, 1123, 1156
AvaI CYCGRG 2 cut(s) 122, 1021
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BalI TGGCCA 3 cut(s) 375, 852, 885
BanII GRGCYC 1 cut(s) 1173
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 2 cut(s) 578, 1173
BbvI GCAGC 9 cut(s) 193, 424, 591, 1042, 1045, 1052, 1064, 1135, 1250
BccI CCATC 1 cut(s) 875
BceAI ACGGC 5 cut(s) 99, 131, 167, 983, 1055
BciVI GTATCC 2 cut(s) 337, 946
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 4 cut(s) 95, 159, 1022, 1023
BcoDI GTCTC 4 cut(s) 58, 190, 909, 1159
BfaI CTAG 3 cut(s) 675, 959, 1166
BfmI CTRYAG 1 cut(s) 581
BfuI GTATCC 2 cut(s) 337, 946
BglII AGATCT 2 cut(s) 700, 1209
BisI GCNGC 9 cut(s) 207, 438, 605, 1053, 1056, 1059, 1066, 1124, 1264
BlsI GCNGC 9 cut(s) 208, 439, 606, 1054, 1057, 1060, 1067, 1125, 1265
Bme1390I CCNGG 4 cut(s) 95, 159, 1022, 1023
BmeT110I CYCGRG 2 cut(s) 122, 1021
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 4 cut(s) 95, 159, 1022, 1023
BmsI GCATC 2 cut(s) 749, 1110
BoxI GACNNNNGTC 1 cut(s) 1151
BpiI GAAGAC 1 cut(s) 938
BpuMI CCSGG 4 cut(s) 95, 159, 1022, 1023
BsaAI YACGTR 1 cut(s) 652
BsaI GGTCTC 2 cut(s) 909, 1159
BsaJI CCNNGG 4 cut(s) 94, 370, 926, 1021
BsaWI WCCGGW 2 cut(s) 214, 808
BsaXI ACNNNNNCTCC 3 cut(s) 33, 38, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 501, 848, 1141
Bse1I ACTGG 2 cut(s) 345, 1036
Bse3DI GCAATG 1 cut(s) 238
BseAI TCCGGA 1 cut(s) 808
BseDI CCNNGG 4 cut(s) 94, 370, 926, 1021
BseGI GGATG 5 cut(s) 136, 486, 805, 838, 1017
BseLI CCNNNNNNNGG 3 cut(s) 501, 848, 1141
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 3 cut(s) 563, 1187, 1197
BseNI ACTGG 2 cut(s) 345, 1036
BseXI GCAGC 9 cut(s) 193, 424, 591, 1042, 1045, 1052, 1064, 1135, 1250
BshFI GGCC 5 cut(s) 121, 375, 421, 852, 885
BsiHKAI GWGCWC 2 cut(s) 578, 1173
BsiHKCI CYCGRG 2 cut(s) 122, 1021
BsiSI CCGG 5 cut(s) 94, 158, 215, 809, 1022
BslI CCNNNNNNNGG 3 cut(s) 501, 848, 1141
BsmAI GTCTC 4 cut(s) 58, 190, 909, 1159
BsmBI CGTCTC 1 cut(s) 190
BsmI GAATGC 2 cut(s) 394, 1340
BsnI GGCC 5 cut(s) 121, 375, 421, 852, 885
Bso31I GGTCTC 2 cut(s) 909, 1159
BsoBI CYCGRG 2 cut(s) 122, 1021
Bsp1286I GDGCHC 2 cut(s) 578, 1173
Bsp13I TCCGGA 1 cut(s) 808
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 4 cut(s) 161, 219, 700, 1209
BspACI CCGC 5 cut(s) 127, 144, 515, 527, 874
BspANI GGCC 5 cut(s) 121, 375, 421, 852, 885
BspCNI CTCAG 3 cut(s) 564, 1188, 1198
BspEI TCCGGA 1 cut(s) 808
BspMAI CTGCAG 1 cut(s) 585
BspPI GGATC 1 cut(s) 169
BspTNI GGTCTC 2 cut(s) 909, 1159
BsrBI CCGCTC 2 cut(s) 127, 517
BsrDI GCAATG 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 2 cut(s) 345, 1036
BssECI CCNNGG 4 cut(s) 94, 370, 926, 1021
BssMI GATC 4 cut(s) 161, 219, 700, 1209
BssNAI GTATAC 2 cut(s) 22, 590
BssT1I CCWWGG 2 cut(s) 370, 926
Bst1107I GTATAC 2 cut(s) 22, 590
Bst4CI ACNGT 5 cut(s) 50, 81, 277, 504, 983
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 978
BstBAI YACGTR 1 cut(s) 652
BstC8I GCNNGC 3 cut(s) 609, 1063, 1128
BstDEI CTNAG 5 cut(s) 572, 899, 968, 1196, 1206
BstF5I GGATG 5 cut(s) 136, 486, 805, 838, 1017
BstHHI GCGC 3 cut(s) 87, 613, 1070
BstKTI GATC 4 cut(s) 164, 222, 703, 1212
BstMAI GTCTC 4 cut(s) 58, 190, 909, 1159
BstMBI GATC 4 cut(s) 161, 219, 700, 1209
BstMWI GCNNNNNNNGC 4 cut(s) 610, 995, 1058, 1067
BstNSI RCATGY 2 cut(s) 611, 793
BstPAI GACNNNNGTC 1 cut(s) 1151
BstSCI CCNGG 4 cut(s) 93, 157, 1020, 1021
BstSFI CTRYAG 1 cut(s) 581
BstV1I GCAGC 9 cut(s) 193, 424, 591, 1042, 1045, 1052, 1064, 1135, 1250
BstV2I GAAGAC 1 cut(s) 938
BstX2I RGATCY 2 cut(s) 700, 1209
BstYI RGATCY 2 cut(s) 700, 1209
BstZ17I GTATAC 2 cut(s) 22, 590
BsuI GTATCC 2 cut(s) 337, 946
BsuRI GGCC 5 cut(s) 121, 375, 421, 852, 885
BtgZI GCGATG 1 cut(s) 1133
BtsCI GGATG 5 cut(s) 136, 486, 805, 838, 1017
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 4 cut(s) 226, 338, 569, 988
Cac8I GCNNGC 3 cut(s) 609, 1063, 1128
CaiI CAGNNNCTG 4 cut(s) 228, 281, 604, 1058
CfoI GCGC 3 cut(s) 87, 613, 1070
Cfr13I GGNCC 1 cut(s) 120
Cfr9I CCCGGG 1 cut(s) 1021
Csp6I GTAC 1 cut(s) 979
CviAII CATG 7 cut(s) 433, 608, 668, 790, 848, 1088, 1108
CviQI GTAC 1 cut(s) 979
DdeI CTNAG 5 cut(s) 572, 899, 968, 1196, 1206
DpnI GATC 4 cut(s) 163, 221, 702, 1211
DpnII GATC 4 cut(s) 161, 219, 700, 1209
DraI TTTAAA 1 cut(s) 499
DraIII CACNNNGTG 2 cut(s) 962, 1111
DrdI GACNNNNNNGTC 1 cut(s) 818
DseDI GACNNNNNNGTC 1 cut(s) 818
EaeI YGGCCR 3 cut(s) 373, 850, 883
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 1171
Eco130I CCWWGG 2 cut(s) 370, 926
Eco24I GRGCYC 1 cut(s) 1173
Eco31I GGTCTC 2 cut(s) 909, 1159
Eco53kI GAGCTC 1 cut(s) 1171
Eco57I CTGAAG 2 cut(s) 426, 1068
Eco88I CYCGRG 2 cut(s) 122, 1021
EcoICRI GAGCTC 1 cut(s) 1171
EcoT14I CCWWGG 2 cut(s) 370, 926
EcoT38I GRGCYC 1 cut(s) 1173
ErhI CCWWGG 2 cut(s) 370, 926
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 7 cut(s) 436, 611, 671, 793, 851, 1091, 1111
FatI CATG 7 cut(s) 432, 607, 667, 789, 847, 1087, 1107
FbaI TGATCA 1 cut(s) 219
FblI GTMKAC 4 cut(s) 21, 485, 589, 1185
Fnu4HI GCNGC 9 cut(s) 207, 438, 605, 1053, 1056, 1059, 1066, 1124, 1264
FokI GGATG 5 cut(s) 123, 493, 792, 825, 1004
FriOI GRGCYC 1 cut(s) 1173
Fsp4HI GCNGC 9 cut(s) 207, 438, 605, 1053, 1056, 1059, 1066, 1124, 1264
FspBI CTAG 3 cut(s) 675, 959, 1166
GlaI GCGC 3 cut(s) 86, 612, 1069
GluI GCNGC 9 cut(s) 207, 438, 605, 1053, 1056, 1059, 1066, 1124, 1264
HaeIII GGCC 5 cut(s) 121, 375, 421, 852, 885
HapII CCGG 5 cut(s) 94, 158, 215, 809, 1022
HhaI GCGC 3 cut(s) 87, 613, 1070
Hin1II CATG 7 cut(s) 436, 611, 671, 793, 851, 1091, 1111
Hin6I GCGC 3 cut(s) 85, 611, 1068
HinP1I GCGC 3 cut(s) 85, 611, 1068
HincII GTYRAC 1 cut(s) 1186
HindII GTYRAC 1 cut(s) 1186
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 8 cut(s) 64, 90, 301, 366, 511, 800, 812, 1250
HpaII CCGG 5 cut(s) 94, 158, 215, 809, 1022
HphI GGTGA 7 cut(s) 59, 206, 229, 650, 974, 1123, 1156
Hpy166II GTNNAC 7 cut(s) 22, 486, 590, 658, 784, 965, 1186
Hpy188I TCNGA 7 cut(s) 11, 224, 247, 480, 688, 1048, 1227
Hpy188III TCNNGA 8 cut(s) 444, 733, 797, 809, 1156, 1195, 1282, 1320
Hpy8I GTNNAC 7 cut(s) 22, 486, 590, 658, 784, 965, 1186
Hpy99I CGWCG 2 cut(s) 271, 274
HpyAV CCTTC 2 cut(s) 191, 1108
HpyCH4III ACNGT 5 cut(s) 50, 81, 277, 504, 983
HpyCH4IV ACGT 3 cut(s) 592, 651, 1242
HpyCH4V TGCA 9 cut(s) 231, 284, 335, 583, 607, 740, 1123, 1266, 1338
HpyF10VI GCNNNNNNNGC 4 cut(s) 610, 995, 1058, 1067
HpyF3I CTNAG 5 cut(s) 572, 899, 968, 1196, 1206
HpySE526I ACGT 3 cut(s) 592, 651, 1242
Hsp92II CATG 7 cut(s) 436, 611, 671, 793, 851, 1091, 1111
HspAI GCGC 3 cut(s) 85, 611, 1068
Kpn2I TCCGGA 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 4 cut(s) 161, 219, 700, 1209
LmnI GCTCC 3 cut(s) 312, 994, 1197
Lsp1109I GCAGC 9 cut(s) 193, 424, 591, 1042, 1045, 1052, 1064, 1135, 1250
LweI GCATC 2 cut(s) 749, 1110
MaeI CTAG 3 cut(s) 675, 959, 1166
MaeII ACGT 3 cut(s) 592, 651, 1242
MaeIII GTNAC 4 cut(s) 638, 1034, 1144, 1199
MalI GATC 4 cut(s) 163, 221, 702, 1211
MbiI CCGCTC 2 cut(s) 127, 517
MboI GATC 4 cut(s) 161, 219, 700, 1209
MboII GAAGA 5 cut(s) 735, 773, 776, 943, 1289
MflI RGATCY 2 cut(s) 700, 1209
MhlI GDGCHC 2 cut(s) 578, 1173
MlsI TGGCCA 3 cut(s) 375, 852, 885
MluCI AATT 5 cut(s) 453, 551, 628, 825, 974
MluNI TGGCCA 3 cut(s) 375, 852, 885
MlyI GAGTC 3 cut(s) 84, 520, 806
MnlI CCTC 5 cut(s) 151, 749, 843, 1001, 1304
Mox20I TGGCCA 3 cut(s) 375, 852, 885
MroI TCCGGA 1 cut(s) 808
MscI TGGCCA 3 cut(s) 375, 852, 885
MseI TTAA 4 cut(s) 41, 498, 644, 1028
MslI CAYNNNNRTG 2 cut(s) 231, 827
Msp20I TGGCCA 3 cut(s) 375, 852, 885
MspA1I CMGCKG 2 cut(s) 529, 1055
MspI CCGG 5 cut(s) 94, 158, 215, 809, 1022
MspR9I CCNGG 4 cut(s) 95, 159, 1022, 1023
Mva1269I GAATGC 2 cut(s) 394, 1340
MwoI GCNNNNNNNGC 4 cut(s) 610, 995, 1058, 1067
NciI CCSGG 4 cut(s) 95, 159, 1022, 1023
NdeII GATC 4 cut(s) 161, 219, 700, 1209
NlaIII CATG 7 cut(s) 436, 611, 671, 793, 851, 1091, 1111
NmuCI GTSAC 3 cut(s) 638, 1034, 1144
NspI RCATGY 2 cut(s) 611, 793
PaeI GCATGC 1 cut(s) 611
PctI GAATGC 2 cut(s) 394, 1340
PfeI GAWTC 5 cut(s) 64, 301, 366, 800, 1250
PflMI CCANNNNNTGG 1 cut(s) 848
PkrI GCNGC 9 cut(s) 208, 439, 606, 1054, 1057, 1060, 1067, 1125, 1265
PleI GAGTC 3 cut(s) 84, 519, 806
PpsI GAGTC 3 cut(s) 84, 519, 806
Ppu21I YACGTR 1 cut(s) 652
PshAI GACNNNNGTC 1 cut(s) 1151
Psp124BI GAGCTC 1 cut(s) 1173
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 585
PstNI CAGNNNCTG 4 cut(s) 228, 281, 604, 1058
PsuI RGATCY 2 cut(s) 700, 1209
PvuII CAGCTG 1 cut(s) 1055
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
RseI CAYNNNNRTG 2 cut(s) 231, 827
SacI GAGCTC 1 cut(s) 1173
SalI GTCGAC 1 cut(s) 1184
SaqAI TTAA 4 cut(s) 41, 498, 644, 1028
SatI GCNGC 9 cut(s) 207, 438, 605, 1053, 1056, 1059, 1066, 1124, 1264
Sau3AI GATC 4 cut(s) 161, 219, 700, 1209
Sau96I GGNCC 1 cut(s) 120
SbfI CCTGCAGG 1 cut(s) 585
SchI GAGTC 3 cut(s) 84, 520, 806
ScrFI CCNGG 4 cut(s) 95, 159, 1022, 1023
SdaI CCTGCAGG 1 cut(s) 585
SduI GDGCHC 2 cut(s) 578, 1173
SfaNI GCATC 2 cut(s) 749, 1110
SfcI CTRYAG 1 cut(s) 581
SmaI CCCGGG 1 cut(s) 1023
SmiMI CAYNNNNRTG 2 cut(s) 231, 827
SphI GCATGC 1 cut(s) 611
Sse8387I CCTGCAGG 1 cut(s) 585
Sse9I AATT 5 cut(s) 453, 551, 628, 825, 974
SsiI CCGC 5 cut(s) 127, 144, 515, 527, 874
SspMI CTAG 3 cut(s) 675, 959, 1166
SstI GAGCTC 1 cut(s) 1173
StyD4I CCNGG 4 cut(s) 93, 157, 1020, 1021
StyI CCWWGG 2 cut(s) 370, 926
TaaI ACNGT 5 cut(s) 50, 81, 277, 504, 983
TaiI ACGT 3 cut(s) 595, 654, 1245
TaqI TCGA 5 cut(s) 509, 798, 835, 1157, 1185
TaqII GACCGA 1 cut(s) 122
TasI AATT 5 cut(s) 453, 551, 628, 825, 974
TatI WGTACW 1 cut(s) 978
TfiI GAWTC 5 cut(s) 64, 301, 366, 800, 1250
Tru1I TTAA 4 cut(s) 41, 498, 644, 1028
Tru9I TTAA 4 cut(s) 41, 498, 644, 1028
TscAI CASTG 4 cut(s) 233, 345, 576, 988
TseFI GTSAC 3 cut(s) 638, 1034, 1144
TseI GCWGC 9 cut(s) 206, 437, 604, 1052, 1055, 1058, 1065, 1123, 1263
Tsp45I GTSAC 3 cut(s) 638, 1034, 1144
TspDTI ATGAA 7 cut(s) 17, 249, 735, 740, 1006, 1263, 1289
TspGWI ACGGA 1 cut(s) 447
TspMI CCCGGG 1 cut(s) 1021
TspRI CASTG 4 cut(s) 233, 345, 576, 988
Van91I CCANNNNNTGG 1 cut(s) 848
XapI RAATTY 1 cut(s) 551
XceI RCATGY 2 cut(s) 611, 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 1021
XmiI GTMKAC 4 cut(s) 21, 485, 589, 1185
XspI CTAG 3 cut(s) 675, 959, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.