RLG00000024135

3-deoxy-D-manno-octulosonic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008414
Physical Location & Seq
Forward (+)
32347740 .. 32351959
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024135

Sequence Viewer

Length: 1368 bp
ATGGCGGCAACGAAGGGAAAGGTGGTTTACAACATTTACAGAGCTTTGAGCCATGGCCTGTCTCCATTTCTCTACCTCCACCTTCACTTGCGTAAACTCCGAGGCCTCGAGCACCCTCTCCGCTGGCCGGAACGCCTCGGCCGCCCCTCCCTCCCTCGCCCGCCGGGCCCACTCCTCTGGTTCCACGCCGTCTCATTAGGTGAAGGAATGTCTGCGATTCCTGTCATCAAAGAATGCATTCAGCGGAGGCCTGATTTAACCATTCTGATGACCACTACGACTTCGTCTGCATTTGATGTAATAAGGAAACGTCTTCCAACTACTGTGATTCATCAGTTTGCGCCGATTGATACCCCTTCTGCTATGGACACATTCCTTGGTCACTGGAAGCCAAATGCAATTGTGTTAATGGAGAGTGAACTGTGGCCAAATTTGATCATCGGTGCTTCAGAAAAGGGTATCATGCTGGCGTTGTTAAATGCACGGGTATCTACTAAATCTTTTAAACGTTGGTCGGGACCCGTGCTTCTTCCACTTATTTCGTTGATGCTCTCAAAGTTTTCATTGATTGCTCCATTGAGCACTATGCAAGCAATCCATTTTCAGCTACTGCATGCCCCACCTTTTGTCATCAACTTTTCTGGTGACCTGAAATATGTCATTGAAGAATTTGACATGTGTGAGGGAGAGATCAGAAGTATAGAAGATTTGACGGTTCACCTTGCCCACAGGCAGGTTTGGATGGCTTCTTCCATTCATAAGGGGGAAGAAGAAGTAATACTAGGCATTCACAAAGTGCTCATGCAAGAATATCCCGATCTGTTCACAATTATTGTGCCTCGACATCCACACCATGGAAAAGAGATTGCTAATAAATTGTGGAGAGAAGGGCATGGCGTGGCTTTGAGGTCTCAGCATGATAAACTTGTTGGAGGAACTAATATATTGGTGGTGGACACATTAGGTGAACTGAGACACTTGTATAGGTTAACTCCAGTAGCTGTAATTGGAGGTTCCTTCTTTCCTAGTCTTTCTGGTCACAACATATCAGAAGCTGCTGCAGCTGGCTGTGCCATTTTGACTGGTCCACATGTTGGGCATTTTTCATCCATGGTACTGGAAATGCAACAACTAAATCCTCTGTCAGTCCTACAGGTTTCTGGTCGATTGGAACTTGAAGAAGCTCTCAGAAAGTTATTGAGTGATGTTAAAATTCTAGAAGAACATCGATTAGCTGCAAAACAAGTGTATCATGCTCTGTCGAGTGGAATTGTTGGGAATGTCTGGAAGCTACTAGATTTGCATATTCTCCAACGCAATTTGAATTTAAAAGGAAATAAAAATCAGCATGCAATAATAGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00540 ko01100 map00540 map01100
Pfam Domains
Protein Families
Glycos_transf_N

Protein Analysis

456

Amino Acids

50.84

Weight (kDa)

9.31

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_N PF04413 41 - 220 3.3e-54 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 724
AccB7I CCANNNNNTGG 2 cut(s) 854, 1094
AciI CCGC 5 cut(s) 5, 121, 142, 161, 244
AclI AACGTT 1 cut(s) 508
AcoI YGGCCR 3 cut(s) 125, 139, 425
AcsI RAATTY 4 cut(s) 430, 668, 1212, 1324
AcuI CTGAAG 1 cut(s) 432
AdeI CACNNNGTG 1 cut(s) 796
AfaI GTAC 1 cut(s) 1116
AfiI CCNNNNNNNGG 4 cut(s) 127, 733, 854, 1094
AflIII ACRYGT 2 cut(s) 675, 1090
AgsI TTSAA 3 cut(s) 665, 1178, 1324
AluBI AGCT 8 cut(s) 44, 607, 1001, 1055, 1064, 1184, 1235, 1291
AluI AGCT 8 cut(s) 44, 607, 1001, 1055, 1064, 1184, 1235, 1291
Alw21I GWGCWC 3 cut(s) 114, 584, 801
Alw26I GTCTC 4 cut(s) 66, 196, 915, 967
AlwNI CAGNNNCTG 3 cut(s) 610, 1001, 1055
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 7 cut(s) 55, 103, 125, 139, 166, 248, 425
ApaI GGGCCC 1 cut(s) 170
ApeKI GCWGC 4 cut(s) 1055, 1058, 1061, 1235
ApoI RAATTY 4 cut(s) 430, 668, 1212, 1324
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 4 cut(s) 166, 167, 518, 1085
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 4 cut(s) 212, 656, 710, 977
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 2 cut(s) 518, 1085
BaeGI GKGCMC 1 cut(s) 170
BaeI ACNNNNGTAYC 2 cut(s) 1232, 1265
BalI TGGCCA 1 cut(s) 427
BanII GRGCYC 1 cut(s) 170
BarI GAAGNNNNNNTAC 2 cut(s) 762, 794
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 3 cut(s) 114, 584, 801
BbvI GCAGC 4 cut(s) 1042, 1045, 1073, 1222
BccI CCATC 1 cut(s) 736
BceAI ACGGC 1 cut(s) 173
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 4 cut(s) 66, 196, 915, 967
BfaI CTAG 4 cut(s) 782, 1026, 1217, 1295
BfmI CTRYAG 2 cut(s) 1059, 1151
BfuAI ACCTGC 1 cut(s) 724
BglI GCCNNNNNGGC 1 cut(s) 165
BisI GCNGC 6 cut(s) 6, 142, 1056, 1059, 1062, 1236
BlsI GCNGC 6 cut(s) 7, 143, 1057, 1060, 1063, 1237
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 2 cut(s) 518, 1085
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 4 cut(s) 166, 167, 518, 1085
BmiI GGNNCC 5 cut(s) 168, 182, 519, 520, 1015
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 978
BpuMI CCSGG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 1228
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 6 cut(s) 52, 100, 136, 376, 853, 1110
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 733, 854, 1094
Bse1I ACTGG 4 cut(s) 389, 995, 1087, 1122
BseCI ATCGAT 1 cut(s) 1228
BseDI CCNNGG 6 cut(s) 52, 100, 136, 376, 853, 1110
BseGI GGATG 3 cut(s) 747, 844, 1106
BseLI CCNNNNNNNGG 4 cut(s) 127, 733, 854, 1094
BseMII CTCAG 3 cut(s) 926, 962, 1201
BseNI ACTGG 4 cut(s) 389, 995, 1087, 1122
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 170
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 4 cut(s) 1042, 1045, 1073, 1222
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 7 cut(s) 57, 105, 127, 141, 168, 250, 427
BshVI ATCGAT 1 cut(s) 1228
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 3 cut(s) 114, 584, 801
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 128, 164
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 4 cut(s) 127, 733, 854, 1094
BsmAI GTCTC 4 cut(s) 66, 196, 915, 967
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 531
BsmI GAATGC 3 cut(s) 237, 239, 786
BsnI GGCC 7 cut(s) 57, 105, 127, 141, 168, 250, 427
Bso31I GGTCTC 1 cut(s) 915
BsoBI CYCGRG 1 cut(s) 107
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 4 cut(s) 114, 170, 584, 801
Bsp143I GATC 3 cut(s) 435, 690, 817
Bsp19I CCATGG 3 cut(s) 52, 853, 1110
BspACI CCGC 5 cut(s) 5, 121, 142, 161, 244
BspANI GGCC 7 cut(s) 57, 105, 127, 141, 168, 250, 427
BspCNI CTCAG 3 cut(s) 925, 963, 1200
BspDI ATCGAT 1 cut(s) 1228
BspLI GGNNCC 5 cut(s) 168, 182, 519, 520, 1015
BspMAI CTGCAG 1 cut(s) 1063
BspMI ACCTGC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 915
BsrI ACTGG 4 cut(s) 389, 995, 1087, 1122
BssECI CCNNGG 6 cut(s) 52, 100, 136, 376, 853, 1110
BssMI GATC 3 cut(s) 435, 690, 817
BssT1I CCWWGG 4 cut(s) 52, 376, 853, 1110
Bst4CI ACNGT 3 cut(s) 325, 423, 715
BstC8I GCNNGC 7 cut(s) 125, 161, 468, 591, 615, 1066, 1350
BstDEI CTNAG 3 cut(s) 912, 971, 1187
BstDSI CCRYGG 3 cut(s) 52, 853, 1110
BstEII GGTNACC 1 cut(s) 644
BstF5I GGATG 3 cut(s) 747, 844, 1106
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 3 cut(s) 438, 693, 820
BstMAI GTCTC 4 cut(s) 66, 196, 915, 967
BstMBI GATC 3 cut(s) 435, 690, 817
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 4 cut(s) 141, 165, 1061, 1070
BstNSI RCATGY 4 cut(s) 617, 679, 1094, 1352
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 2 cut(s) 1059, 1151
BstSLI GKGCMC 1 cut(s) 170
BstV1I GCAGC 4 cut(s) 1042, 1045, 1073, 1222
BstV2I GAAGAC 1 cut(s) 305
BstXI CCANNNNNNTGG 2 cut(s) 177, 1117
BstZI CGGCCG 1 cut(s) 139
Bsu15I ATCGAT 1 cut(s) 1228
BsuRI GGCC 7 cut(s) 57, 105, 127, 141, 168, 250, 427
BsuTUI ATCGAT 1 cut(s) 1228
BtgI CCRYGG 3 cut(s) 52, 853, 1110
BtsCI GGATG 3 cut(s) 747, 844, 1106
BtsIMutI CAGTG 1 cut(s) 382
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 7 cut(s) 125, 161, 468, 591, 615, 1066, 1350
CaiI CAGNNNCTG 3 cut(s) 610, 1001, 1055
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 4 cut(s) 166, 167, 518, 1085
ClaI ATCGAT 1 cut(s) 1228
Csp6I GTAC 1 cut(s) 1115
CviQI GTAC 1 cut(s) 1115
DdeI CTNAG 3 cut(s) 912, 971, 1187
DpnI GATC 3 cut(s) 437, 692, 819
DpnII GATC 3 cut(s) 435, 690, 817
DraI TTTAAA 2 cut(s) 505, 1329
DraIII CACNNNGTG 1 cut(s) 796
EaeI YGGCCR 3 cut(s) 125, 139, 425
EagI CGGCCG 1 cut(s) 139
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 4 cut(s) 52, 376, 853, 1110
Eco147I AGGCCT 2 cut(s) 105, 250
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 915
Eco47I GGWCC 2 cut(s) 518, 1085
Eco52I CGGCCG 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 107
Eco91I GGTNACC 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 644
EcoT14I CCWWGG 4 cut(s) 52, 376, 853, 1110
EcoT22I ATGCAT 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 4 cut(s) 52, 376, 853, 1110
Esp3I CGTCTC 1 cut(s) 196
FaqI GGGAC 1 cut(s) 531
FauI CCCGC 1 cut(s) 168
FbaI TGATCA 1 cut(s) 435
Fnu4HI GCNGC 6 cut(s) 6, 142, 1056, 1059, 1062, 1236
FokI GGATG 3 cut(s) 754, 831, 1093
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 6 cut(s) 6, 142, 1056, 1059, 1062, 1236
FspBI CTAG 4 cut(s) 782, 1026, 1217, 1295
GlaI GCGC 1 cut(s) 342
GluI GCNGC 6 cut(s) 6, 142, 1056, 1059, 1062, 1236
GsuI CTGGAG 1 cut(s) 978
HaeIII GGCC 7 cut(s) 57, 105, 127, 141, 168, 250, 427
HapII CCGG 2 cut(s) 128, 164
HhaI GCGC 1 cut(s) 343
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HinfI GANTC 2 cut(s) 217, 328
HpaI GTTAAC 1 cut(s) 990
HpaII CCGG 2 cut(s) 128, 164
HphI GGTGA 4 cut(s) 212, 656, 710, 977
Hpy166II GTNNAC 9 cut(s) 28, 95, 419, 718, 825, 955, 968, 990, 1088
Hpy188I TCNGA 6 cut(s) 101, 267, 451, 695, 1051, 1190
Hpy188III TCNNGA 4 cut(s) 516, 815, 1217, 1285
Hpy8I GTNNAC 9 cut(s) 28, 95, 419, 718, 825, 955, 968, 990, 1088
HpyAV CCTTC 6 cut(s) 7, 92, 197, 366, 881, 1027
HpyCH4III ACNGT 3 cut(s) 325, 423, 715
HpyCH4IV ACGT 2 cut(s) 310, 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 165, 1061, 1070
HpyF3I CTNAG 3 cut(s) 912, 971, 1187
HpySE526I ACGT 2 cut(s) 310, 508
HspAI GCGC 1 cut(s) 341
KflI GGGWCCC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 435
KspAI GTTAAC 1 cut(s) 990
Kzo9I GATC 3 cut(s) 435, 690, 817
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 4 cut(s) 1042, 1045, 1073, 1222
LweI GCATC 1 cut(s) 537
MaeI CTAG 4 cut(s) 782, 1026, 1217, 1295
MaeII ACGT 2 cut(s) 310, 508
MaeIII GTNAC 3 cut(s) 380, 644, 1037
MalI GATC 3 cut(s) 437, 692, 819
MboI GATC 3 cut(s) 435, 690, 817
MboII GAAGA 9 cut(s) 305, 521, 677, 716, 741, 779, 782, 1190, 1232
MfeI CAATTG 1 cut(s) 399
MhlI GDGCHC 4 cut(s) 114, 170, 584, 801
MlsI TGGCCA 1 cut(s) 427
MluNI TGGCCA 1 cut(s) 427
MmeI TCCRAC 3 cut(s) 341, 910, 1336
Mox20I TGGCCA 1 cut(s) 427
Mph1103I ATGCAT 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 237
MscI TGGCCA 1 cut(s) 427
MseI TTAA 7 cut(s) 257, 407, 476, 504, 989, 1209, 1328
MslI CAYNNNNRTG 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 427
MspA1I CMGCKG 3 cut(s) 123, 244, 1064
MspI CCGG 2 cut(s) 128, 164
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 3 cut(s) 237, 239, 786
MwoI GCNNNNNNNGC 4 cut(s) 141, 165, 1061, 1070
NciI CCSGG 1 cut(s) 165
NcoI CCATGG 3 cut(s) 52, 853, 1110
NdeII GATC 3 cut(s) 435, 690, 817
NlaIV GGNNCC 5 cut(s) 168, 182, 519, 520, 1015
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 3 cut(s) 380, 644, 1037
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 4 cut(s) 617, 679, 1094, 1352
PaeI GCATGC 2 cut(s) 617, 1352
PaeR7I CTCGAG 1 cut(s) 107
PceI AGGCCT 2 cut(s) 105, 250
PciI ACATGT 2 cut(s) 675, 1090
PctI GAATGC 3 cut(s) 237, 239, 786
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 217, 328
PflFI GACNNNGTC 1 cut(s) 283
PflMI CCANNNNNTGG 2 cut(s) 854, 1094
PkrI GCNGC 6 cut(s) 7, 143, 1057, 1060, 1063, 1237
PpuMI RGGWCCY 1 cut(s) 518
PscI ACATGT 2 cut(s) 675, 1090
Psp1406I AACGTT 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 518
PspEI GGTNACC 1 cut(s) 644
PspN4I GGNNCC 5 cut(s) 168, 182, 519, 520, 1015
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 4 cut(s) 166, 167, 518, 1085
PspPPI RGGWCCY 1 cut(s) 518
PspXI VCTCGAGB 1 cut(s) 107
PstI CTGCAG 1 cut(s) 1063
PstNI CAGNNNCTG 3 cut(s) 610, 1001, 1055
PsyI GACNNNGTC 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 1 cut(s) 1116
RsaNI GTAC 1 cut(s) 1115
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 7 cut(s) 257, 407, 476, 504, 989, 1209, 1328
SatI GCNGC 6 cut(s) 6, 142, 1056, 1059, 1062, 1236
Sau3AI GATC 3 cut(s) 435, 690, 817
Sau96I GGNCC 4 cut(s) 166, 167, 518, 1085
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 4 cut(s) 114, 170, 584, 801
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 2 cut(s) 1059, 1151
Sfr274I CTCGAG 1 cut(s) 107
SinI GGWCC 2 cut(s) 518, 1085
SlaI CTCGAG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SphI GCATGC 2 cut(s) 617, 1352
SseBI AGGCCT 2 cut(s) 105, 250
SsiI CCGC 5 cut(s) 5, 121, 142, 161, 244
SspMI CTAG 4 cut(s) 782, 1026, 1217, 1295
StuI AGGCCT 2 cut(s) 105, 250
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 4 cut(s) 52, 376, 853, 1110
TaaI ACNGT 3 cut(s) 325, 423, 715
TaiI ACGT 2 cut(s) 313, 511
TaqI TCGA 5 cut(s) 108, 841, 1165, 1228, 1262
TauI GCSGC 2 cut(s) 8, 144
TfiI GAWTC 2 cut(s) 217, 328
Tru1I TTAA 7 cut(s) 257, 407, 476, 504, 989, 1209, 1328
Tru9I TTAA 7 cut(s) 257, 407, 476, 504, 989, 1209, 1328
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 3 cut(s) 380, 644, 1037
TseI GCWGC 4 cut(s) 1055, 1058, 1061, 1235
Tsp45I GTSAC 3 cut(s) 380, 644, 1037
TspDTI ATGAA 4 cut(s) 320, 552, 746, 1095
TspRI CASTG 1 cut(s) 389
Tth111I GACNNNGTC 1 cut(s) 283
Van91I CCANNNNNTGG 2 cut(s) 854, 1094
VpaK11BI GGWCC 2 cut(s) 518, 1085
XapI RAATTY 4 cut(s) 430, 668, 1212, 1324
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 4 cut(s) 617, 679, 1094, 1352
XhoI CTCGAG 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 4 cut(s) 782, 1026, 1217, 1295
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.