AT5G05830

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013092
Physical Location & Seq
Forward (+)
1755669 .. 1757085
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05830.1

Sequence Viewer

Length: 615 bp
ATGGAATCCGTACAGCCACATCTCGAGACCTTTGTCTCTGTTAAAGTAATCAATAGCTCAAGTGGCTGTGATGGCGATGGAGAGCCTCGTCGGAGCTCCGGTGCGGTGGAGAAGGAGGAAGAAATAGTGAAAGATGAGGAGAGGAGATCCAATGCGTCGGTGTGTTCGGTGGAAATAGATCTGGAGCTTGGATTGCCTGAGAAAGTTGTACATTTGTCGCAATCTGAGAGAGATTGCAGGATTTGTCACATGAGCTTAGATGCAGCGAATCTTGAATCTGGTGTTCCCATTGAGCTTGGCTGTTCTTGTAAAGCTGATCTCGCTGCTGCTCATAAACACTGTGCTGAGACTTGGTTCAAGATCAAAGGAAACAAAATCTGTGAAGTTTGTGGGTCGATTGCGGGTAATGTTGTTGGAAGTGTTGAGGTGGAGTCTGAGGAAAGCCGGAATGAAGCAAATGGTGTAGAGAATCTAACTCTGAGAACATCTGGTCCACGATTGGTGGAAGGTAGAAGCTTCTGGCAAGGTCACCGGTTCTTGAATTTCCTTTTAGCTTGTATGGTCTTTGCATTTGTTATCTCATGGCTCTTCCACTTCAATGTTCCTTCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

204

Amino Acids

22.37

Weight (kDa)

5.11

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 79 - 130 2.2e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05830
fragaria_vesca FvH4_7g23740 FvH4_7g23740
malus_domestica MD01G1144600.v1.1 MD07G1211200.v1.1
prunus_persica Prupe.2G244000_v2.0.a1
pyrus_communis pycom01g16420 pycom07g19470
rosa_chinensis RchiOBHm_Chr1g0369041
rosa_laevigata RLG00000027162
rosa_multiflora Rmu_sc0004250.1_g000028
rosa_roxburghii Rroxscaffold_4G00288060
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG354200 Rh1BG317600 Rh1CG332100 Rh1DG348500
rosa_wichuraiana Rw1G031290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 104, 401
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 541
AfaI GTAC 2 cut(s) 12, 210
AgeI ACCGGT 1 cut(s) 531
AgsI TTSAA 4 cut(s) 275, 358, 541, 598
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
AluBI AGCT 8 cut(s) 57, 96, 187, 255, 295, 314, 516, 554
AluI AGCT 8 cut(s) 57, 96, 187, 255, 295, 314, 516, 554
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 3 cut(s) 20, 40, 341
AlwI GGATC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 23
ApeKI GCWGC 3 cut(s) 263, 323, 326
ApoI RAATTY 1 cut(s) 541
AsiGI ACCGGT 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 491
AsuHPI GGTGA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 275, 310, 313
BccI CCATC 2 cut(s) 65, 71
BcoDI GTCTC 3 cut(s) 20, 40, 341
BglII AGATCT 1 cut(s) 178
BisI GCNGC 3 cut(s) 264, 324, 327
BlsI GCNGC 3 cut(s) 265, 325, 328
Bme18I GGWCC 1 cut(s) 491
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 491
BmsI GCATC 1 cut(s) 250
BoxI GACNNNNGTC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 43
BsaI GGTCTC 1 cut(s) 20
BsaWI WCCGGW 2 cut(s) 98, 531
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bse118I RCCGGY 1 cut(s) 531
BseMII CTCAG 5 cut(s) 189, 216, 336, 426, 470
BseRI GAGGAG 2 cut(s) 152, 157
BseXI GCAGC 3 cut(s) 275, 310, 313
BshTI ACCGGT 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 3 cut(s) 99, 445, 532
BsmAI GTCTC 3 cut(s) 20, 40, 341
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 98
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 4 cut(s) 146, 178, 316, 360
BspACI CCGC 2 cut(s) 104, 401
BspCNI CTCAG 5 cut(s) 190, 217, 337, 427, 471
BspPI GGATC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 531
BsrGI TGTACA 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 531
BssMI GATC 4 cut(s) 146, 178, 316, 360
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 208
BstDEI CTNAG 6 cut(s) 198, 225, 256, 345, 435, 479
BstEII GGTNACC 1 cut(s) 527
BstKTI GATC 4 cut(s) 149, 181, 319, 363
BstMAI GTCTC 3 cut(s) 20, 40, 341
BstMBI GATC 4 cut(s) 146, 178, 316, 360
BstMWI GCNNNNNNNGC 4 cut(s) 63, 72, 193, 320
BstPAI GACNNNNGTC 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 527
BstV1I GCAGC 3 cut(s) 275, 310, 313
BstX2I RGATCY 2 cut(s) 146, 178
BstYI RGATCY 2 cut(s) 146, 178
BtgZI GCGATG 1 cut(s) 90
BtsIMutI CAGTG 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 491
CseI GACGC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 11, 209
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 2 cut(s) 250, 582
CviQI GTAC 2 cut(s) 11, 209
DdeI CTNAG 6 cut(s) 198, 225, 256, 345, 435, 479
DpnI GATC 4 cut(s) 148, 180, 318, 362
DpnII GATC 4 cut(s) 146, 178, 316, 360
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Ecl136II GAGCTC 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 23
Eco91I GGTNACC 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 96
EcoO65I GGTNACC 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 98
FaeI CATG 2 cut(s) 253, 585
FaiI YATR 5 cut(s) 251, 333, 560, 583, 613
FatI CATG 2 cut(s) 249, 581
FauI CCCGC 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 264, 324, 327
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 3 cut(s) 264, 324, 327
GluI GCNGC 3 cut(s) 264, 324, 327
GsuI CTGGAG 1 cut(s) 203
HapII CCGG 3 cut(s) 99, 445, 532
HgaI GACGC 1 cut(s) 144
Hin1II CATG 2 cut(s) 253, 585
HindIII AAGCTT 1 cut(s) 514
HinfI GANTC 5 cut(s) 5, 268, 275, 431, 469
HpaII CCGG 3 cut(s) 99, 445, 532
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 1 cut(s) 494
Hpy188I TCNGA 4 cut(s) 93, 226, 436, 480
Hpy188III TCNNGA 6 cut(s) 23, 25, 182, 272, 358, 538
Hpy8I GTNNAC 1 cut(s) 494
Hpy99I CGWCG 2 cut(s) 93, 160
HpyAV CCTTC 3 cut(s) 106, 500, 615
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4V TGCA 3 cut(s) 237, 263, 569
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 72, 193, 320
HpyF3I CTNAG 6 cut(s) 198, 225, 256, 345, 435, 479
Hsp92II CATG 2 cut(s) 253, 585
Kzo9I GATC 4 cut(s) 146, 178, 316, 360
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 3 cut(s) 93, 101, 184
LpnPI CCDG 9 cut(s) 112, 167, 210, 223, 264, 458, 474, 505, 545
Lsp1109I GCAGC 3 cut(s) 275, 310, 313
LweI GCATC 1 cut(s) 250
MaeIII GTNAC 2 cut(s) 245, 527
MalI GATC 4 cut(s) 148, 180, 318, 362
MboI GATC 4 cut(s) 146, 178, 316, 360
MboII GAAGA 2 cut(s) 131, 580
MflI RGATCY 2 cut(s) 146, 178
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 1 cut(s) 541
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 71, 394
MnlI CCTC 6 cut(s) 96, 109, 130, 135, 418, 430
MseI TTAA 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 597
MspI CCGG 3 cut(s) 99, 445, 532
MwoI GCNNNNNNNGC 4 cut(s) 63, 72, 193, 320
NdeII GATC 4 cut(s) 146, 178, 316, 360
NlaIII CATG 2 cut(s) 253, 585
NmuCI GTSAC 2 cut(s) 245, 527
PaeR7I CTCGAG 1 cut(s) 23
PciSI GCTCTTC 1 cut(s) 593
PfeI GAWTC 4 cut(s) 5, 268, 275, 469
PinAI ACCGGT 1 cut(s) 531
PkrI GCNGC 3 cut(s) 265, 325, 328
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
PshAI GACNNNNGTC 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 98
PspEI GGTNACC 1 cut(s) 527
PspPI GGNCC 1 cut(s) 491
PsuI RGATCY 2 cut(s) 146, 178
RsaI GTAC 2 cut(s) 12, 210
RsaNI GTAC 2 cut(s) 11, 209
RseI CAYNNNNRTG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 98
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 3 cut(s) 264, 324, 327
Sau3AI GATC 4 cut(s) 146, 178, 316, 360
Sau96I GGNCC 1 cut(s) 491
SchI GAGTC 1 cut(s) 440
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 1 cut(s) 250
Sfr274I CTCGAG 1 cut(s) 23
SinI GGWCC 1 cut(s) 491
SlaI CTCGAG 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 2 cut(s) 23, 58
SmoI CTYRAG 2 cut(s) 23, 58
Sse9I AATT 1 cut(s) 541
SsiI CCGC 2 cut(s) 104, 401
SstI GAGCTC 1 cut(s) 98
TaaI ACNGT 1 cut(s) 341
TaqI TCGA 2 cut(s) 24, 395
TasI AATT 1 cut(s) 541
TatI WGTACW 1 cut(s) 208
TfiI GAWTC 4 cut(s) 5, 268, 275, 469
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 1 cut(s) 344
TseFI GTSAC 2 cut(s) 245, 527
TseI GCWGC 3 cut(s) 263, 323, 326
Tsp45I GTSAC 2 cut(s) 245, 527
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 1 cut(s) 541
XhoI CTCGAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.