Rh1CG332100

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0013092
Physical Location & Seq
Reverse (-)
60633280 .. 60636828
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG332100.1

Sequence Viewer

Length: 636 bp
ATGGCATCCAAAACCCAAGTTGTAGTTGTTGATGTTGAGCAAGGAGGTCGCCGGAGCTCGGGTCATGGGTACTCGGATTCCGGGTCAGATGAGGAGGAGGAGGAGGAGCACAGGAGCTCGTGTGTGTCCGGGAGTAGTGAAGAGATTGTGGAGAGGGGATCCTCTGAGAATGAGTCTTCGGTAGAGGAGGAGGTGGATCTGGAATGTGGAGGGGTGCCTGCTGAAGTGAAGGTCCACGTGGCAAAAGTGAAGAGGGATTGTAGGATTTGTCATCTGAGCATGGATGCAACCAATCATGAATCTGGGATTCCTATCGAGTTAGGGTGTTCTTGTAAGGAGGACTTGGCTGCTGCACATAAACAGTGTGCTGAGGCCTGGTTCAAAATCAAGGGTAACAAAACCTGTGAGATCTGCGGATCAACTGCACGCAATGTTTCTGGGGTGAATGAAGCTGAGTTGGCAGAGCAGTGGAATGAAGCAAATGATACTGCAGTGCCACCAGCACCAGTTGCCAGTACACAGCCAGCAGAGAACCGAAACTTCTGGCAGGGTCATCGGTTCCTCAACTTCTTGCTAGCTTGTATGGTCTTCGCCTTTGTCATCTCTTGGCTCTTTCACTTCAATGTACCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

211

Amino Acids

23.0

Weight (kDa)

4.81

Isoelectric Point (pI)

61.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 87 - 138 1.8e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05830
fragaria_vesca FvH4_7g23740 FvH4_7g23740
malus_domestica MD01G1144600.v1.1 MD07G1211200.v1.1
prunus_persica Prupe.2G244000_v2.0.a1
pyrus_communis pycom01g16420 pycom07g19470
rosa_chinensis RchiOBHm_Chr1g0369041
rosa_laevigata RLG00000027162
rosa_multiflora Rmu_sc0004250.1_g000028
rosa_roxburghii Rroxscaffold_4G00288060
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG354200 Rh1BG317600 Rh1CG332100 Rh1DG348500
rosa_wichuraiana Rw1G031290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 214
AciI CCGC 1 cut(s) 414
AclWI GGATC 4 cut(s) 153, 166, 204, 424
AcuI CTGAAG 1 cut(s) 243
AcvI CACGTG 1 cut(s) 238
AfaI GTAC 3 cut(s) 71, 517, 627
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 2 cut(s) 382, 622
AjnI CCWGG 1 cut(s) 374
AluBI AGCT 4 cut(s) 57, 117, 452, 578
AluI AGCT 4 cut(s) 57, 117, 452, 578
Alw21I GWGCWC 3 cut(s) 59, 111, 119
AlwI GGATC 4 cut(s) 153, 166, 204, 424
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 347, 350
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 2 cut(s) 82, 130
AsuHPI GGTGA 1 cut(s) 454
AsuNHI GCTAGC 1 cut(s) 574
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 1 cut(s) 232
BaeI ACNNNNGTAYC 2 cut(s) 477, 510
BamHI GGATCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 214
BanII GRGCYC 2 cut(s) 59, 119
BauI CACGAG 1 cut(s) 118
BbrPI CACGTG 1 cut(s) 238
BbsI GAAGAC 2 cut(s) 168, 580
Bbv12I GWGCWC 3 cut(s) 59, 111, 119
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 2 cut(s) 334, 337
BcgI CGANNNNNNTGC 4 cut(s) 29, 63, 536, 570
BciT130I CCWGG 1 cut(s) 376
BcnI CCSGG 2 cut(s) 82, 130
BfaI CTAG 1 cut(s) 575
BfmI CTRYAG 1 cut(s) 489
BglII AGATCT 1 cut(s) 408
BisI GCNGC 2 cut(s) 348, 351
BlsI GCNGC 2 cut(s) 349, 352
Bme1390I CCNGG 3 cut(s) 82, 130, 376
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 3 cut(s) 160, 216, 560
BmrFI CCNGG 3 cut(s) 82, 130, 376
BmsI GCATC 2 cut(s) 14, 274
BmtI GCTAGC 1 cut(s) 578
BpiI GAAGAC 2 cut(s) 168, 580
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 2 cut(s) 82, 130
BsaAI YACGTR 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 311
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 106, 136
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 506, 513
Bse3DI GCAATG 1 cut(s) 436
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 1 cut(s) 376
BseGI GGATG 2 cut(s) 5, 289
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 4 cut(s) 156, 266, 360, 444
BseNI ACTGG 2 cut(s) 506, 513
BseRI GAGGAG 7 cut(s) 107, 110, 113, 116, 119, 200, 203
BseXI GCAGC 2 cut(s) 334, 337
BsgI GTGCAG 2 cut(s) 336, 408
BshFI GGCC 1 cut(s) 374
BshNI GGYRCC 1 cut(s) 214
BsiHKAI GWGCWC 3 cut(s) 59, 111, 119
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 3 cut(s) 52, 81, 129
BslI CCNNNNNNNGG 1 cut(s) 58
BsnI GGCC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 58
Bsp1286I GDGCHC 3 cut(s) 59, 111, 119
Bsp143I GATC 4 cut(s) 158, 196, 408, 416
BspACI CCGC 1 cut(s) 414
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 4 cut(s) 157, 267, 361, 445
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 3 cut(s) 160, 216, 560
BspMAI CTGCAG 1 cut(s) 493
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 4 cut(s) 153, 166, 204, 424
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 2 cut(s) 506, 513
BssMI GATC 4 cut(s) 158, 196, 408, 416
BssSI CACGAG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 376
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 2 cut(s) 135, 245
BstAPI GCANNNNNTGC 1 cut(s) 509
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 219, 427, 525, 576
BstDEI CTNAG 4 cut(s) 165, 275, 369, 453
BstF5I GGATG 2 cut(s) 5, 289
BstKTI GATC 4 cut(s) 161, 199, 411, 419
BstMBI GATC 4 cut(s) 158, 196, 408, 416
BstMWI GCNNNNNNNGC 2 cut(s) 458, 509
BstNI CCWGG 1 cut(s) 376
BstSCI CCNGG 3 cut(s) 80, 128, 374
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 334, 337
BstV2I GAAGAC 2 cut(s) 168, 580
BstX2I RGATCY 3 cut(s) 158, 196, 408
BstYI RGATCY 3 cut(s) 158, 196, 408
BsuRI GGCC 1 cut(s) 374
BtsCI GGATG 2 cut(s) 5, 289
BtsI GCAGTG 2 cut(s) 473, 498
BtsIMutI CAGTG 3 cut(s) 368, 473, 498
Cac8I GCNNGC 4 cut(s) 219, 427, 525, 576
CciI TCATGA 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 3 cut(s) 70, 516, 626
CviAII CATG 3 cut(s) 65, 280, 296
CviJI RGCY 8 cut(s) 57, 117, 347, 374, 452, 523, 578, 610
CviKI_1 RGCY 8 cut(s) 57, 117, 347, 374, 452, 523, 578, 610
CviQI GTAC 3 cut(s) 70, 516, 626
DdeI CTNAG 4 cut(s) 165, 275, 369, 453
DpnI GATC 4 cut(s) 160, 198, 410, 418
DpnII GATC 4 cut(s) 158, 196, 408, 416
Eam1104I CTCTTC 2 cut(s) 135, 245
EarI CTCTTC 2 cut(s) 135, 245
Ecl136II GAGCTC 2 cut(s) 57, 117
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 2 cut(s) 59, 119
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 2 cut(s) 57, 117
Eco57I CTGAAG 1 cut(s) 243
Eco72I CACGTG 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 58
EcoICRI GAGCTC 2 cut(s) 57, 117
EcoRII CCWGG 1 cut(s) 374
EcoT38I GRGCYC 2 cut(s) 59, 119
FaeI CATG 3 cut(s) 68, 283, 299
FaiI YATR 6 cut(s) 66, 281, 297, 357, 584, 634
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FatI CATG 3 cut(s) 64, 279, 295
Fnu4HI GCNGC 2 cut(s) 348, 351
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 2 cut(s) 59, 119
Fsp4HI GCNGC 2 cut(s) 348, 351
FspBI CTAG 1 cut(s) 575
GluI GCNGC 2 cut(s) 348, 351
HaeIII GGCC 1 cut(s) 374
HapII CCGG 3 cut(s) 52, 81, 129
Hin1II CATG 3 cut(s) 68, 283, 299
HinfI GANTC 4 cut(s) 77, 173, 299, 307
HpaII CCGG 3 cut(s) 52, 81, 129
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 2 cut(s) 235, 518
Hpy188I TCNGA 4 cut(s) 76, 88, 166, 276
Hpy188III TCNNGA 2 cut(s) 200, 296
Hpy8I GTNNAC 2 cut(s) 235, 518
HpyAV CCTTC 1 cut(s) 223
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 4 cut(s) 287, 353, 425, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 509
HpyF3I CTNAG 4 cut(s) 165, 275, 369, 453
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 3 cut(s) 68, 283, 299
Kzo9I GATC 4 cut(s) 158, 196, 408, 416
LmnI GCTCC 3 cut(s) 54, 106, 114
Lsp1109I GCAGC 2 cut(s) 334, 337
LweI GCATC 2 cut(s) 14, 274
MaeI CTAG 1 cut(s) 575
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 392
MalI GATC 4 cut(s) 160, 198, 410, 418
MboI GATC 4 cut(s) 158, 196, 408, 416
MboII GAAGA 4 cut(s) 152, 168, 262, 580
MflI RGATCY 3 cut(s) 158, 196, 408
MhlI GDGCHC 3 cut(s) 59, 111, 119
MlyI GAGTC 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 621
MspI CCGG 3 cut(s) 52, 81, 129
MspR9I CCNGG 3 cut(s) 82, 130, 376
MvaI CCWGG 1 cut(s) 376
MwoI GCNNNNNNNGC 2 cut(s) 458, 509
NciI CCSGG 2 cut(s) 82, 130
NdeII GATC 4 cut(s) 158, 196, 408, 416
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 3 cut(s) 68, 283, 299
NlaIV GGNNCC 3 cut(s) 160, 216, 560
PagI TCATGA 1 cut(s) 295
PceI AGGCCT 1 cut(s) 374
PfeI GAWTC 3 cut(s) 77, 299, 307
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 2 cut(s) 349, 352
PleI GAGTC 1 cut(s) 181
PmaCI CACGTG 1 cut(s) 238
PmlI CACGTG 1 cut(s) 238
PpsI GAGTC 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 238
Psp124BI GAGCTC 2 cut(s) 59, 119
Psp6I CCWGG 1 cut(s) 374
PspCI CACGTG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 374
PspN4I GGNNCC 3 cut(s) 160, 216, 560
PspPI GGNCC 1 cut(s) 232
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 3 cut(s) 158, 196, 408
RsaI GTAC 3 cut(s) 71, 517, 627
RsaNI GTAC 3 cut(s) 70, 516, 626
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 2 cut(s) 59, 119
SatI GCNGC 2 cut(s) 348, 351
Sau3AI GATC 4 cut(s) 158, 196, 408, 416
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 82, 130, 376
SduI GDGCHC 3 cut(s) 59, 111, 119
SetI ASST 9 cut(s) 49, 59, 119, 195, 234, 240, 404, 454, 580
SfaNI GCATC 2 cut(s) 14, 274
SfcI CTRYAG 1 cut(s) 489
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 621
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 1 cut(s) 414
SspMI CTAG 1 cut(s) 575
SstI GAGCTC 2 cut(s) 59, 119
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 80, 128, 374
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 1 cut(s) 240
TaqI TCGA 1 cut(s) 315
TatI WGTACW 1 cut(s) 515
TfiI GAWTC 3 cut(s) 77, 299, 307
TscAI CASTG 3 cut(s) 368, 473, 498
TseI GCWGC 2 cut(s) 347, 350
TspDTI ATGAA 3 cut(s) 312, 462, 489
TspRI CASTG 3 cut(s) 368, 473, 498
VpaK11BI GGWCC 1 cut(s) 232
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.