MD07G1211200.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013092
Physical Location & Seq
Reverse (-)
28984212 .. 28987510
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1211200.v1.1.491

Sequence Viewer

Length: 621 bp
ATGGCAGCCGCAGACAAATCCCACGTGGTTGTTGATGTGGAGGAGGAGGAAGTGGGACGGCGCAGCGCCGCCACGGGTCGAAATAGCTACTGTGACTCCGGCTCCGAGGGGTCGGAGGGCCAGGAGGGGAGATGCTCCAGTGAGTCAGGGTCTGAGATTGTGGAGAGGGAATCCTGTGTGTCGGAGAGTTCGGTAGAGGTGGATCTGGAGGAGGTGAAGATGCACGTGGCGAAAGTGGAGAGGGATTGCAGGATTTGTCATCTGAGCATGGATGCAACGAATCAGGAATCTGGGATTCCTATTGAGCTGGGGTGTTCTTGTAAGGCTGATTTGGCTGCTGCCCACAAGCAGTGTGCTGAGGCTTGGTTCAAGATTAAGGGTAACAAAACCTGTGAGATCTGTGGATCAACCGCACGTAATGTTTCTGGTTTGAGTGAAGCTGAGTTGGCGGAGCAGTGGCATGATGAAGCCGCACTGGCAACAACAGCAGGAGGTGCCCCTGTGCAGACAGCAGAGAGCCGAAACTTCTGGCAGGGTCACCGCTTCCTGAACTTCTTGCTAGCTTGTATGGTCTTTGCGTTTGTTATCTCGTGGCTGTTTCACTTCAATGTGCCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

207

Amino Acids

22.31

Weight (kDa)

4.83

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 83 - 134 6.5e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05830
fragaria_vesca FvH4_7g23740 FvH4_7g23740
malus_domestica MD01G1144600.v1.1 MD07G1211200.v1.1
prunus_persica Prupe.2G244000_v2.0.a1
pyrus_communis pycom01g16420 pycom07g19470
rosa_chinensis RchiOBHm_Chr1g0369041
rosa_laevigata RLG00000027162
rosa_multiflora Rmu_sc0004250.1_g000028
rosa_roxburghii Rroxscaffold_4G00288060
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG354200 Rh1BG317600 Rh1CG332100 Rh1DG348500
rosa_wichuraiana Rw1G031290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AciI CCGC 6 cut(s) 9, 69, 411, 449, 471, 541
AclWI GGATC 2 cut(s) 210, 412
AcvI CACGTG 2 cut(s) 25, 226
AgsI TTSAA 2 cut(s) 370, 607
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 4 cut(s) 87, 307, 440, 563
AluI AGCT 4 cut(s) 87, 307, 440, 563
AlwI GGATC 2 cut(s) 210, 412
AlwNI CAGNNNCTG 1 cut(s) 152
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 4 cut(s) 5, 63, 335, 338
AspLEI GCGC 2 cut(s) 63, 68
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 226, 530
AsuNHI GCTAGC 1 cut(s) 559
BaeGI GKGCMC 2 cut(s) 499, 615
BanI GGYRCC 1 cut(s) 494
BauI CACGAG 1 cut(s) 589
BbrPI CACGTG 2 cut(s) 25, 226
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 4 cut(s) 17, 75, 322, 325
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 122
BfaI CTAG 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 69
BglI GCCNNNNNGGC 1 cut(s) 476
BglII AGATCT 1 cut(s) 396
BisI GCNGC 7 cut(s) 6, 9, 64, 69, 336, 339, 471
BlsI GCNGC 7 cut(s) 7, 10, 65, 70, 337, 340, 472
Bme1390I CCNGG 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 2 cut(s) 103, 496
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 3 cut(s) 122, 210, 262
BmtI GCTAGC 1 cut(s) 563
BpmI CTGGAG 2 cut(s) 121, 227
Bpu10I CCTNAGC 1 cut(s) 357
BsaAI YACGTR 3 cut(s) 25, 226, 416
BsaJI CCNNGG 2 cut(s) 72, 105
BsaXI ACNNNNNCTCC 4 cut(s) 80, 86, 110, 116
Bse1I ACTGG 2 cut(s) 138, 480
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 2 cut(s) 72, 105
BseGI GGATG 1 cut(s) 277
BseMII CTCAG 4 cut(s) 144, 254, 348, 432
BseNI ACTGG 2 cut(s) 138, 480
BseRI GAGGAG 3 cut(s) 56, 59, 224
BseSI GKGCMC 2 cut(s) 499, 615
BseXI GCAGC 4 cut(s) 17, 75, 322, 325
BseYI CCCAGC 1 cut(s) 307
BsgI GTGCAG 1 cut(s) 524
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 494
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 499, 615
Bsp143I GATC 3 cut(s) 202, 396, 404
BspACI CCGC 6 cut(s) 9, 69, 411, 449, 471, 541
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 4 cut(s) 145, 255, 349, 433
BspHI TCATGA 1 cut(s) 617
BspLI GGNNCC 2 cut(s) 103, 496
BspOI GCTAGC 1 cut(s) 563
BspPI GGATC 2 cut(s) 210, 412
BspT107I GGYRCC 1 cut(s) 494
BsrI ACTGG 2 cut(s) 138, 480
BssECI CCNNGG 2 cut(s) 72, 105
BssMI GATC 3 cut(s) 202, 396, 404
BssSI CACGAG 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 589
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 3 cut(s) 25, 226, 416
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 4 cut(s) 153, 263, 357, 441
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 536
BstF5I GGATG 1 cut(s) 277
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 63, 68
BstKTI GATC 3 cut(s) 205, 399, 407
BstMBI GATC 3 cut(s) 202, 396, 404
BstMWI GCNNNNNNNGC 5 cut(s) 332, 446, 476, 485, 494
BstNI CCWGG 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 120
BstSLI GKGCMC 2 cut(s) 499, 615
BstV1I GCAGC 4 cut(s) 17, 75, 322, 325
BstX2I RGATCY 2 cut(s) 202, 396
BstYI RGATCY 2 cut(s) 202, 396
BsuRI GGCC 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 277
BtsI GCAGTG 2 cut(s) 356, 461
BtsIMutI CAGTG 4 cut(s) 145, 356, 461, 473
Cac8I GCNNGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 152
CciI TCATGA 1 cut(s) 617
CfoI GCGC 2 cut(s) 63, 68
Cfr13I GGNCC 1 cut(s) 118
CviAII CATG 3 cut(s) 268, 461, 618
DdeI CTNAG 4 cut(s) 153, 263, 357, 441
DpnI GATC 3 cut(s) 204, 398, 406
DpnII GATC 3 cut(s) 202, 396, 404
EciI GGCGGA 1 cut(s) 464
Eco72I CACGTG 2 cut(s) 25, 226
Eco91I GGTNACC 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 120
FaeI CATG 3 cut(s) 271, 464, 621
FaiI YATR 4 cut(s) 269, 462, 569, 619
FaqI GGGAC 1 cut(s) 69
FatI CATG 3 cut(s) 267, 460, 617
Fnu4HI GCNGC 7 cut(s) 6, 9, 64, 69, 336, 339, 471
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 7 cut(s) 6, 9, 64, 69, 336, 339, 471
FspBI CTAG 1 cut(s) 560
GlaI GCGC 2 cut(s) 62, 67
GluI GCNGC 7 cut(s) 6, 9, 64, 69, 336, 339, 471
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 2 cut(s) 121, 227
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 99
HhaI GCGC 2 cut(s) 63, 68
Hin1II CATG 3 cut(s) 271, 464, 621
Hin6I GCGC 2 cut(s) 61, 66
HinP1I GCGC 2 cut(s) 61, 66
HinfI GANTC 6 cut(s) 95, 143, 170, 280, 287, 295
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 226, 530
Hpy188I TCNGA 5 cut(s) 106, 115, 154, 184, 264
Hpy188III TCNNGA 5 cut(s) 206, 284, 370, 547, 618
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 3 cut(s) 24, 225, 415
HpyCH4V TGCA 4 cut(s) 223, 249, 275, 505
HpyF10VI GCNNNNNNNGC 5 cut(s) 332, 446, 476, 485, 494
HpyF3I CTNAG 4 cut(s) 153, 263, 357, 441
HpySE526I ACGT 3 cut(s) 24, 225, 415
Hsp92II CATG 3 cut(s) 271, 464, 621
HspAI GCGC 2 cut(s) 61, 66
Kzo9I GATC 3 cut(s) 202, 396, 404
LmnI GCTCC 3 cut(s) 107, 140, 451
Lsp1109I GCAGC 4 cut(s) 17, 75, 322, 325
LweI GCATC 3 cut(s) 122, 210, 262
MaeI CTAG 1 cut(s) 560
MaeII ACGT 3 cut(s) 24, 225, 415
MaeIII GTNAC 3 cut(s) 92, 380, 536
MalI GATC 3 cut(s) 204, 398, 406
MboI GATC 3 cut(s) 202, 396, 404
MboII GAAGA 1 cut(s) 229
MflI RGATCY 2 cut(s) 202, 396
MhlI GDGCHC 2 cut(s) 499, 615
MlyI GAGTC 2 cut(s) 89, 152
MmeI TCCRAC 2 cut(s) 93, 162
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 606
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 122
MteI GCGCNGCGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 5 cut(s) 332, 446, 476, 485, 494
NdeII GATC 3 cut(s) 202, 396, 404
NheI GCTAGC 1 cut(s) 559
NlaIII CATG 3 cut(s) 271, 464, 621
NlaIV GGNNCC 2 cut(s) 103, 496
NmuCI GTSAC 2 cut(s) 92, 536
PagI TCATGA 1 cut(s) 617
PfeI GAWTC 4 cut(s) 170, 280, 287, 295
PkrI GCNGC 7 cut(s) 7, 10, 65, 70, 337, 340, 472
PleI GAGTC 2 cut(s) 89, 151
PmaCI CACGTG 2 cut(s) 25, 226
PmlI CACGTG 2 cut(s) 25, 226
PpsI GAGTC 2 cut(s) 89, 151
Ppu21I YACGTR 3 cut(s) 25, 226, 416
Psp6I CCWGG 1 cut(s) 120
PspCI CACGTG 2 cut(s) 25, 226
PspEI GGTNACC 1 cut(s) 536
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 103, 496
PspPI GGNCC 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 2 cut(s) 202, 396
RseI CAYNNNNRTG 1 cut(s) 606
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 7 cut(s) 6, 9, 64, 69, 336, 339, 471
Sau3AI GATC 3 cut(s) 202, 396, 404
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 2 cut(s) 89, 152
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 2 cut(s) 499, 615
SfaNI GCATC 3 cut(s) 122, 210, 262
SmiMI CAYNNNNRTG 1 cut(s) 606
SsiI CCGC 6 cut(s) 9, 69, 411, 449, 471, 541
SspMI CTAG 1 cut(s) 560
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 3 cut(s) 27, 228, 418
TaqI TCGA 1 cut(s) 79
TauI GCSGC 3 cut(s) 11, 71, 473
TfiI GAWTC 4 cut(s) 170, 280, 287, 295
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 4 cut(s) 145, 356, 461, 480
TseFI GTSAC 2 cut(s) 92, 536
TseI GCWGC 4 cut(s) 5, 63, 335, 338
Tsp45I GTSAC 2 cut(s) 92, 536
TspDTI ATGAA 1 cut(s) 480
TspRI CASTG 4 cut(s) 145, 356, 461, 480
XspI CTAG 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.