AT5G07360

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004947
Physical Location & Seq
Reverse (-)
2326687 .. 2330462
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G07360.2

Sequence Viewer

Length: 1980 bp
ATGGCGAGACCATCGATCGCCACGTGTGATCCAAGCGCACGACCTTGTTTAGCGACGCGTGGCTCACTTCCTCGTAGCCGTACATGCGCGCCGCTTTTATTCCTCTGCCTCCTCCTAGGTTTAACGGTCTTTTGTGCGGACGCAAATCCTTCTGACTCGAAGCTTCTCTGCTCCCGTCTTCTTCCATGTGAGCCTTCTATTGAAATGGTGAGAAATTGTAAGAATCTGTGGAAACTCTTGGACCGGAAAGTGAGTGTTCCTTGCCGTAAGATTACAACTTTGACGAGGACAGTTACTCTAACTGAACTACCAGATAAGACTATGGAAGCTAAGTGTCTTCAGGGCATGTTTGAGTTGATTGATTCTGCATTCTTCAATGAAACTAAACTACAGGAGATTGCAAAGGGGGCTACTGAAATGAATGTTCCTATATATCGAGCCAACCGGAAACTAGTAGCTACTAAGAATGGTGGGTTGGAAAATCCATCCCCGTTGGTGTTTAATCCGTCTTGGAACAGAGAAGTTCAACGAGTGCAAGACAAGAGATTCAAATACCCTTCGGCATCCGGAGTAAAACTCCCTAGAGATGAAGAAGATATCGCCTTCATGAGCGTTCTTGAACTCGGAGAACTTATAAAGACGAGACAAATTACTTCCAAAGAACTCGTTCGGATTTATCTTAAGCAGTTAAAACGGTACAATCATGTTCTTGAAGCGGTAGTTACTTATACTGAAGAACTGGCATACAAACAAGCCAAAGAAGCTGATGATTTACTCTCTCAAGGAACTTATCTTGGACCTCTTCACGGGATTCCATATGGTTTAAAGGATATAGTTGCAGTTCCCGGATATAAAACAACTTGGGGTTCAACTTCTTTCAAAGACCAATTTCTTGACATTGAAGCATGGGTTTACAAAAGATTAAAAGCTTCAGGTGCAGTTTTAGTAGCAAAGCTTGTTACGGGTTCTATGGCTTATGATGATATCTGGTTCGGGGGACGGACTAGGAATCCATGGAATATCGAGGAATTCTCTACCGGGTCATCAGCTGGACCCGCTGCATCCACATCGGCTGGTATGGTTCCTTTTGCTATAGGCTCGGAGACAGCAGGCTCAATGACATACCCTGCAGCGCGGTGTGGCATAACAGCATTGCGTCCGACATTTGGGAGCGTTGGTAGAACCGGAGTGATGAGCATATCCGAGAGCCTGGATAAGCTGGGACCTTTCTGTAGAACAGCAGCGGATTGCGCTGTAATTCTTGACGCTATCAAAGGAAAAGATCCCGATGATCTCTCATCTAGAGAGATCGCGTTTGAAGATCCCTTCTCCGTTGATATCACAAAACTCACAGTTGGGTATACAAAAGATGCTGATATGAAGGTGGTGGAAGTTCTTGGTTCCAAAGGTGTCAATATGGTTCCTTTTGAGCTAAATTATACTGTGGAATCGGTTCAAGGGATATTGAACTTCACAATGGATGTAGATATGTTGGCTCATTTTGATGAATGGCAAAGAACCGGTCAAGAAGATCTCTATGAAGCTCAAGATCAATGGCCGGTTGAGTTACGCCGTGCTCGTGTAGTTACAGCAGTAGATTACATTCAGGCTCAGAGAGCTCGTGGTAAGTTGATTCGGGAAGTGGAGAAGAGCTTTACAGTCGATGCGTTTATTGGAAATGTGACCGATTGGGAGAAAGTCTGTATGGGAAACCTAGTCGGTTTGCCGGTTCTAGTGATTCCGACCGGTTTCAAGAACATAACGGATCCACCAACCAACAGTTGCCGGAGACGGACAACGATCAATGCCGGAATTTATGCCCCTCCAGAACGTGATCACATTGCTTTGGCATTGGGTATGGCGTACCAATCGGTTACCGATGCACATAGGAAGCGACCACCTATTGATGATCTTGGACCGGATGACTCGATACCAAATCCTCCTCGGGCTTTAATCCCTCCCCGTAGGTTACACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidase

Protein Analysis

659

Amino Acids

72.95

Weight (kDa)

8.05

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 221 - 545 1.7e-47 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AccI GTMKAC 1 cut(s) 1361
AccII CGCG 4 cut(s) 58, 89, 1135, 1313
AccIII TCCGGA 1 cut(s) 566
AciI CCGC 6 cut(s) 92, 137, 716, 1056, 1135, 1244
AclWI GGATC 5 cut(s) 23, 1277, 1316, 1760, 1773
AcoI YGGCCR 1 cut(s) 1556
AcsI RAATTY 2 cut(s) 1028, 1812
AcuI CTGAAG 3 cut(s) 323, 753, 915
AcvI CACGTG 1 cut(s) 24
AfaI GTAC 3 cut(s) 82, 698, 1865
AfiI CCNNNNNNNGG 2 cut(s) 806, 1166
AflII CTTAAG 1 cut(s) 680
AflIII ACRYGT 2 cut(s) 23, 56
AgeI ACCGGT 2 cut(s) 1520, 1745
AhlI ACTAGT 1 cut(s) 451
AjnI CCWGG 1 cut(s) 1209
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
Alw21I GWGCWC 2 cut(s) 1579, 1621
Alw26I GTCTC 3 cut(s) 637, 1097, 1783
AlwI GGATC 5 cut(s) 23, 1277, 1316, 1760, 1773
Ama87I CYCGRG 1 cut(s) 1944
Aor13HI TCCGGA 1 cut(s) 566
AoxI GGCC 1 cut(s) 1556
ApeKI GCWGC 3 cut(s) 1058, 1130, 1241
ApoI RAATTY 2 cut(s) 1028, 1812
ArsI GACNNNNNNTTYG 2 cut(s) 1507, 1539
AsiGI ACCGGT 2 cut(s) 1520, 1745
Asp700I GAANNNNTTC 2 cut(s) 666, 1452
AspA2I CCTAGG 1 cut(s) 115
AspLEI GCGC 5 cut(s) 38, 89, 91, 1135, 1253
AspS9I GGNCC 5 cut(s) 241, 797, 1052, 1223, 1916
AsuC2I CCSGG 2 cut(s) 846, 1039
AsuHPI GGTGA 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 1944
AvaII GGWCC 5 cut(s) 241, 797, 1052, 1223, 1916
AvrII CCTAGG 1 cut(s) 115
BamHI GGATCC 1 cut(s) 1765
BanII GRGCYC 1 cut(s) 1621
BauI CACGAG 2 cut(s) 1578, 1620
BbrPI CACGTG 1 cut(s) 24
BbsI GAAGAC 2 cut(s) 170, 329
Bbv12I GWGCWC 2 cut(s) 1579, 1621
BbvI GCAGC 3 cut(s) 1045, 1142, 1253
BccI CCATC 2 cut(s) 19, 493
BceAI ACGGC 3 cut(s) 63, 249, 1557
BcgI CGANNNNNNTGC 2 cut(s) 1050, 1084
BciT130I CCWGG 1 cut(s) 1211
BclI TGATCA 1 cut(s) 1834
BcnI CCSGG 2 cut(s) 846, 1039
BcoDI GTCTC 3 cut(s) 637, 1097, 1783
BcuI ACTAGT 1 cut(s) 451
BfaI CTAG 7 cut(s) 116, 452, 582, 1005, 1302, 1715, 1733
BfmI CTRYAG 4 cut(s) 389, 1092, 1128, 1231
BfrI CTTAAG 1 cut(s) 680
BglII AGATCT 1 cut(s) 1531
BisI GCNGC 4 cut(s) 92, 1059, 1131, 1242
BlnI CCTAGG 1 cut(s) 115
BlsI GCNGC 4 cut(s) 93, 1060, 1132, 1243
Bme1390I CCNGG 3 cut(s) 846, 1039, 1211
Bme18I GGWCC 5 cut(s) 241, 797, 1052, 1223, 1916
BmeT110I CYCGRG 1 cut(s) 1944
BmgT120I GGNCC 5 cut(s) 241, 797, 1052, 1223, 1916
BmiI GGNNCC 6 cut(s) 1054, 1083, 1224, 1402, 1422, 1767
BmrFI CCNGG 3 cut(s) 846, 1039, 1211
BmsI GCATC 5 cut(s) 572, 1070, 1360, 1654, 1870
BpiI GAAGAC 2 cut(s) 170, 329
BplI GAGNNNNNCTC 4 cut(s) 561, 593, 1016, 1048
BpmI CTGGAG 1 cut(s) 1809
BpuEI CTTGAG 2 cut(s) 765, 1530
BpuMI CCSGG 2 cut(s) 846, 1039
Bsa29I ATCGAT 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 115, 1013, 1943
BsaWI WCCGGW 7 cut(s) 243, 444, 566, 1184, 1520, 1745, 1918
BsaXI ACNNNNNCTCC 2 cut(s) 1162, 1192
Bsc4I CCNNNNNNNGG 2 cut(s) 806, 1166
Bse118I RCCGGY 4 cut(s) 1520, 1558, 1726, 1745
Bse1I ACTGG 1 cut(s) 744
Bse3DI GCAATG 2 cut(s) 1151, 1839
BseAI TCCGGA 1 cut(s) 566
BseBI CCWGG 1 cut(s) 1211
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 115, 1013, 1943
BseGI GGATG 5 cut(s) 485, 563, 1061, 1486, 1927
BseLI CCNNNNNNNGG 2 cut(s) 806, 1166
BseMI GCAATG 2 cut(s) 1151, 1839
BseMII CTCAG 1 cut(s) 1625
BseNI ACTGG 1 cut(s) 744
BsePI GCGCGC 1 cut(s) 87
BseRI GAGGAG 2 cut(s) 101, 1932
BseXI GCAGC 3 cut(s) 1045, 1142, 1253
BseYI CCCAGC 1 cut(s) 1219
BsgI GTGCAG 1 cut(s) 957
Bsh1236I CGCG 4 cut(s) 58, 89, 1135, 1313
Bsh1285I CGRYCG 2 cut(s) 18, 1746
BshFI GGCC 1 cut(s) 1558
BshTI ACCGGT 2 cut(s) 1520, 1745
BshVI ATCGAT 1 cut(s) 14
BsiEI CGRYCG 2 cut(s) 18, 1746
BsiHKAI GWGCWC 2 cut(s) 1579, 1621
BsiHKCI CYCGRG 1 cut(s) 1944
BslFI GGGAC 2 cut(s) 1011, 1236
BslI CCNNNNNNNGG 2 cut(s) 806, 1166
BsmAI GTCTC 3 cut(s) 637, 1097, 1783
BsmBI CGTCTC 1 cut(s) 1783
BsmFI GGGAC 2 cut(s) 1011, 1236
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 1 cut(s) 1558
BsoBI CYCGRG 1 cut(s) 1944
Bsp1286I GDGCHC 2 cut(s) 1579, 1621
Bsp13I TCCGGA 1 cut(s) 566
Bsp19I CCATGG 1 cut(s) 1013
BspACI CCGC 6 cut(s) 92, 137, 716, 1056, 1135, 1244
BspANI GGCC 1 cut(s) 1558
BspCNI CTCAG 1 cut(s) 1624
BspDI ATCGAT 1 cut(s) 14
BspEI TCCGGA 1 cut(s) 566
BspFNI CGCG 4 cut(s) 58, 89, 1135, 1313
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 6 cut(s) 1054, 1083, 1224, 1402, 1422, 1767
BspMAI CTGCAG 1 cut(s) 1132
BspPI GGATC 5 cut(s) 23, 1277, 1316, 1760, 1773
BspQI GCTCTTC 1 cut(s) 1643
BspTI CTTAAG 1 cut(s) 680
BsrDI GCAATG 2 cut(s) 1151, 1839
BsrFI RCCGGY 4 cut(s) 1520, 1558, 1726, 1745
BsrI ACTGG 1 cut(s) 744
BssAI RCCGGY 4 cut(s) 1520, 1558, 1726, 1745
BssECI CCNNGG 3 cut(s) 115, 1013, 1943
BssHII GCGCGC 1 cut(s) 87
BssNAI GTATAC 1 cut(s) 1362
BssSI CACGAG 2 cut(s) 1578, 1620
BssT1I CCWWGG 2 cut(s) 115, 1013
Bst1107I GTATAC 1 cut(s) 1362
Bst2BI CACGAG 2 cut(s) 1578, 1620
Bst2UI CCWGG 1 cut(s) 1211
Bst4CI ACNGT 7 cut(s) 127, 292, 696, 1354, 1444, 1660, 1781
Bst6I CTCTTC 2 cut(s) 807, 1643
BstAFI CTTAAG 1 cut(s) 680
BstBAI YACGTR 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 89, 1111
BstDEI CTNAG 3 cut(s) 330, 462, 1611
BstDSI CCRYGG 1 cut(s) 1013
BstEII GGTNACC 1 cut(s) 1873
BstF5I GGATG 5 cut(s) 485, 563, 1061, 1486, 1927
BstFNI CGCG 4 cut(s) 58, 89, 1135, 1313
BstHHI GCGC 5 cut(s) 38, 89, 91, 1135, 1253
BstMAI GTCTC 3 cut(s) 637, 1097, 1783
BstMCI CGRYCG 2 cut(s) 18, 1746
BstMWI GCNNNNNNNGC 7 cut(s) 84, 407, 761, 935, 1055, 1250, 1616
BstNI CCWGG 1 cut(s) 1211
BstNSI RCATGY 2 cut(s) 87, 349
BstPI GGTNACC 1 cut(s) 1873
BstSCI CCNGG 3 cut(s) 844, 1037, 1209
BstSFI CTRYAG 4 cut(s) 389, 1092, 1128, 1231
BstUI CGCG 4 cut(s) 58, 89, 1135, 1313
BstV1I GCAGC 3 cut(s) 1045, 1142, 1253
BstV2I GAAGAC 2 cut(s) 170, 329
BstX2I RGATCY 4 cut(s) 1282, 1321, 1531, 1765
BstYI RGATCY 4 cut(s) 1282, 1321, 1531, 1765
BstZ17I GTATAC 1 cut(s) 1362
Bsu15I ATCGAT 1 cut(s) 14
BsuRI GGCC 1 cut(s) 1558
BsuTUI ATCGAT 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 1013
BtsCI GGATG 5 cut(s) 485, 563, 1061, 1486, 1927
Cac8I GCNNGC 2 cut(s) 89, 1111
CciI TCATGA 1 cut(s) 606
CfoI GCGC 5 cut(s) 38, 89, 91, 1135, 1253
Cfr10I RCCGGY 4 cut(s) 1520, 1558, 1726, 1745
Cfr13I GGNCC 5 cut(s) 241, 797, 1052, 1223, 1916
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 4 cut(s) 64, 149, 1145, 1274
Csp6I GTAC 3 cut(s) 81, 697, 1864
CspAI ACCGGT 2 cut(s) 1520, 1745
CviAII CATG 7 cut(s) 84, 186, 346, 607, 704, 906, 1014
CviQI GTAC 3 cut(s) 81, 697, 1864
DdeI CTNAG 3 cut(s) 330, 462, 1611
DraI TTTAAA 1 cut(s) 825
EaeI YGGCCR 1 cut(s) 1556
Eam1104I CTCTTC 2 cut(s) 807, 1643
EarI CTCTTC 2 cut(s) 807, 1643
Ecl136II GAGCTC 1 cut(s) 1619
Eco130I CCWWGG 2 cut(s) 115, 1013
Eco24I GRGCYC 1 cut(s) 1621
Eco32I GATATC 3 cut(s) 598, 985, 1339
Eco47I GGWCC 5 cut(s) 241, 797, 1052, 1223, 1916
Eco53kI GAGCTC 1 cut(s) 1619
Eco57I CTGAAG 3 cut(s) 323, 753, 915
Eco72I CACGTG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 1944
Eco91I GGTNACC 1 cut(s) 1873
EcoICRI GAGCTC 1 cut(s) 1619
EcoO109I RGGNCCY 1 cut(s) 1223
EcoO65I GGTNACC 1 cut(s) 1873
EcoRI GAATTC 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 1209
EcoRV GATATC 3 cut(s) 598, 985, 1339
EcoT14I CCWWGG 2 cut(s) 115, 1013
EcoT38I GRGCYC 1 cut(s) 1621
ErhI CCWWGG 2 cut(s) 115, 1013
Esp3I CGTCTC 1 cut(s) 1783
FaeI CATG 7 cut(s) 87, 189, 349, 610, 707, 909, 1017
FaqI GGGAC 2 cut(s) 1011, 1236
FatI CATG 7 cut(s) 83, 185, 345, 606, 703, 905, 1013
FauI CCCGC 1 cut(s) 1063
FauNDI CATATG 1 cut(s) 817
FbaI TGATCA 1 cut(s) 1834
FblI GTMKAC 1 cut(s) 1361
Fnu4HI GCNGC 4 cut(s) 92, 1059, 1131, 1242
FokI GGATG 5 cut(s) 472, 550, 1048, 1493, 1934
FriOI GRGCYC 1 cut(s) 1621
Fsp4HI GCNGC 4 cut(s) 92, 1059, 1131, 1242
FspBI CTAG 7 cut(s) 116, 452, 582, 1005, 1302, 1715, 1733
GlaI GCGC 5 cut(s) 37, 88, 90, 1134, 1252
GluI GCNGC 4 cut(s) 92, 1059, 1131, 1242
GsaI CCCAGC 1 cut(s) 1223
GsuI CTGGAG 1 cut(s) 1809
HaeIII GGCC 1 cut(s) 1558
HgaI GACGC 4 cut(s) 64, 149, 1145, 1274
HhaI GCGC 5 cut(s) 38, 89, 91, 1135, 1253
Hin1II CATG 7 cut(s) 87, 189, 349, 610, 707, 909, 1017
Hin6I GCGC 5 cut(s) 36, 87, 89, 1133, 1251
HinP1I GCGC 5 cut(s) 36, 87, 89, 1133, 1251
HindIII AAGCTT 3 cut(s) 161, 927, 953
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 913, 1362
Hpy188I TCNGA 8 cut(s) 154, 626, 672, 1102, 1161, 1204, 1614, 1743
Hpy8I GTNNAC 2 cut(s) 913, 1362
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 6 cut(s) 159, 204, 567, 613, 1336, 1375
HpyCH4III ACNGT 7 cut(s) 127, 292, 696, 1354, 1444, 1660, 1781
HpyCH4IV ACGT 2 cut(s) 23, 1831
HpyCH4V TGCA 8 cut(s) 368, 401, 535, 839, 938, 1061, 1130, 1883
HpyF10VI GCNNNNNNNGC 7 cut(s) 84, 407, 761, 935, 1055, 1250, 1616
HpyF3I CTNAG 3 cut(s) 330, 462, 1611
HpySE526I ACGT 2 cut(s) 23, 1831
Hsp92II CATG 7 cut(s) 87, 189, 349, 610, 707, 909, 1017
HspAI GCGC 5 cut(s) 36, 87, 89, 1133, 1251
Kpn2I TCCGGA 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 1834
LguI GCTCTTC 1 cut(s) 1643
LmnI GCTCC 2 cut(s) 176, 1170
Lsp1109I GCAGC 3 cut(s) 1045, 1142, 1253
LweI GCATC 5 cut(s) 572, 1070, 1360, 1654, 1870
MaeI CTAG 7 cut(s) 116, 452, 582, 1005, 1302, 1715, 1733
MaeII ACGT 2 cut(s) 23, 1831
MaeIII GTNAC 8 cut(s) 292, 721, 958, 1566, 1585, 1681, 1873, 1968
MflI RGATCY 4 cut(s) 1282, 1321, 1531, 1765
MhlI GDGCHC 2 cut(s) 1579, 1621
MluCI AATT 7 cut(s) 214, 648, 887, 1028, 1257, 1435, 1812
MluI ACGCGT 1 cut(s) 56
MlyI GAGTC 2 cut(s) 149, 1919
MmeI TCCRAC 3 cut(s) 456, 1184, 1766
MroI TCCGGA 1 cut(s) 566
MroXI GAANNNNTTC 2 cut(s) 666, 1452
MseI TTAA 7 cut(s) 122, 501, 681, 689, 824, 923, 1952
MslI CAYNNNNRTG 1 cut(s) 1503
MspA1I CMGCKG 3 cut(s) 1049, 1058, 1244
MspCI CTTAAG 1 cut(s) 680
MspR9I CCNGG 3 cut(s) 846, 1039, 1211
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 1 cut(s) 1211
MvnI CGCG 4 cut(s) 58, 89, 1135, 1313
MwoI GCNNNNNNNGC 7 cut(s) 84, 407, 761, 935, 1055, 1250, 1616
NciI CCSGG 2 cut(s) 846, 1039
NcoI CCATGG 1 cut(s) 1013
NdeI CATATG 1 cut(s) 817
NlaIII CATG 7 cut(s) 87, 189, 349, 610, 707, 909, 1017
NlaIV GGNNCC 6 cut(s) 1054, 1083, 1224, 1402, 1422, 1767
NmuCI GTSAC 1 cut(s) 1681
NspI RCATGY 2 cut(s) 87, 349
PagI TCATGA 1 cut(s) 606
PauI GCGCGC 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 1643
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 1576
PctI GAATGC 1 cut(s) 368
PdmI GAANNNNTTC 2 cut(s) 666, 1452
PfeI GAWTC 8 cut(s) 223, 362, 546, 811, 1009, 1448, 1633, 1738
PfoI TCCNGGA 1 cut(s) 844
PinAI ACCGGT 2 cut(s) 1520, 1745
PkrI GCNGC 4 cut(s) 93, 1060, 1132, 1243
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 2 cut(s) 149, 1919
PmaCI CACGTG 1 cut(s) 24
PmlI CACGTG 1 cut(s) 24
PpsI GAGTC 2 cut(s) 149, 1919
Ppu21I YACGTR 1 cut(s) 24
PpuMI RGGWCCY 1 cut(s) 1223
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 1621
Psp5II RGGWCCY 1 cut(s) 1223
Psp6I CCWGG 1 cut(s) 1209
PspCI CACGTG 1 cut(s) 24
PspEI GGTNACC 1 cut(s) 1873
PspFI CCCAGC 1 cut(s) 1219
PspGI CCWGG 1 cut(s) 1209
PspN4I GGNNCC 6 cut(s) 1054, 1083, 1224, 1402, 1422, 1767
PspPI GGNCC 5 cut(s) 241, 797, 1052, 1223, 1916
PspPPI RGGWCCY 1 cut(s) 1223
PstI CTGCAG 1 cut(s) 1132
PsuI RGATCY 4 cut(s) 1282, 1321, 1531, 1765
PteI GCGCGC 1 cut(s) 87
PvuI CGATCG 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 3 cut(s) 82, 698, 1865
RsaNI GTAC 3 cut(s) 81, 697, 1864
RseI CAYNNNNRTG 1 cut(s) 1503
SacI GAGCTC 1 cut(s) 1621
SapI GCTCTTC 1 cut(s) 1643
SaqAI TTAA 7 cut(s) 122, 501, 681, 689, 824, 923, 1952
SatI GCNGC 4 cut(s) 92, 1059, 1131, 1242
Sau96I GGNCC 5 cut(s) 241, 797, 1052, 1223, 1916
SchI GAGTC 2 cut(s) 149, 1919
ScrFI CCNGG 3 cut(s) 846, 1039, 1211
SduI GDGCHC 2 cut(s) 1579, 1621
SfaNI GCATC 5 cut(s) 572, 1070, 1360, 1654, 1870
SfcI CTRYAG 4 cut(s) 389, 1092, 1128, 1231
SinI GGWCC 5 cut(s) 241, 797, 1052, 1223, 1916
SmiMI CAYNNNNRTG 1 cut(s) 1503
SmlI CTYRAG 3 cut(s) 680, 780, 1545
SmoI CTYRAG 3 cut(s) 680, 780, 1545
SpeI ACTAGT 1 cut(s) 451
Sse9I AATT 7 cut(s) 214, 648, 887, 1028, 1257, 1435, 1812
SsiI CCGC 6 cut(s) 92, 137, 716, 1056, 1135, 1244
SspMI CTAG 7 cut(s) 116, 452, 582, 1005, 1302, 1715, 1733
SstI GAGCTC 1 cut(s) 1621
StyD4I CCNGG 3 cut(s) 844, 1037, 1209
StyI CCWWGG 2 cut(s) 115, 1013
TaaI ACNGT 7 cut(s) 127, 292, 696, 1354, 1444, 1660, 1781
TaiI ACGT 2 cut(s) 26, 1834
TaqI TCGA 6 cut(s) 14, 158, 436, 1023, 1662, 1928
TaqII GACCGA 1 cut(s) 1700
TasI AATT 7 cut(s) 214, 648, 887, 1028, 1257, 1435, 1812
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 8 cut(s) 223, 362, 546, 811, 1009, 1448, 1633, 1738
Tru1I TTAA 7 cut(s) 122, 501, 681, 689, 824, 923, 1952
Tru9I TTAA 7 cut(s) 122, 501, 681, 689, 824, 923, 1952
TseFI GTSAC 1 cut(s) 1681
TseI GCWGC 3 cut(s) 1058, 1130, 1241
Tsp45I GTSAC 1 cut(s) 1681
TspDTI ATGAA 7 cut(s) 393, 434, 595, 603, 1394, 1521, 1554
TspGWI ACGGA 5 cut(s) 495, 1015, 1321, 1778, 1807
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 5 cut(s) 241, 797, 1052, 1223, 1916
XapI RAATTY 2 cut(s) 1028, 1812
XbaI TCTAGA 1 cut(s) 1301
XceI RCATGY 2 cut(s) 87, 349
XmaJI CCTAGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 1361
XmnI GAANNNNTTC 2 cut(s) 666, 1452
XspI CTAG 7 cut(s) 116, 452, 582, 1005, 1302, 1715, 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.