MD00G1076500.v1.1

glutamyl-tRNA(Gln) amidotransferase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004947
Physical Location & Seq
Reverse (-)
15365241 .. 15368688
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1076500.v1.1.491

Sequence Viewer

Length: 969 bp
ATGCCAACTTCTTCAACGATGAAAAGGTACAATCCTGTTCTTGAGGCTGTGGTTACCTATATTAGAGAATTAGCAAACCAACAAGCAAAAGAAGCCGATGAATTGCTTGCTCGAGGAGTTTACTTGGGTCCTCTCCACGGGATTCCTTATGGACTGAAGGATATAATCTCAGTTCCCCACTACAAAACGACGTGGCGTTCTAAAACTTTCAAAGATAAAGTTCTTGACATCGAAACCTGGGTGTTCAAGAGGTTGAAGTCTGCTGGAGCAATTCTTGTTGCAAAGCTTGTTACTAGAACGCTAGCATACGATGACATCTGGTTTGGTGGTAGGACAAAAAACAAGTGGAATATTGAGGAATTCACAACGGGTTCATCATCTGGTATGGTGCCATTTGCGATTGGATCTGAAACAGTTGCTTCCATGACTTTACCTGCAGCTCATTGTGGCGTGGCAGCTTTGTGTGCAATATTTGCCTCTGTTGGTCGAACCCGCATCATGAGCTTATTAGAAAGCTTGAGACATCCCTTCGAGGATCCTTTCTCGGTGGACATAACAAGGCTTACTGTCGGATATGTTAATGATGTTGACATGAAGGTTATTCATGTTCTTGAATCAAAAAGCATTAATATGGTCCCTTTCAAACTCAACTACATTGTTGACTTTGTACAATGCATTGTAAATTTTACGATGGATGTGGATATGTTGTCTCACTTTGATGAGTGGCAACGGTCCAGGAAGTATGATGATTATGAATCTCAAGCCAATGTCGATTGGGAGAGAATCTGTATGGGGAACCTAGTAGGGATGCCTGTAATTGTTGTTCCGGCAAGATTTACCAATATATTGGGTCCTCCTCGCAGTGACTGCAGTAGAAGAACAGCAATGACCATAGGCATTTACGCTCCGCCTCATCGCGATCACATTGCTCTAGCATTGACAATGGCATACCAATTTGTGACCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidase

Protein Analysis

323

Amino Acids

36.29

Weight (kDa)

8.2

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 7 - 152 3.8e-24 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 1 cut(s) 388
AccII CGCG 1 cut(s) 918
AciI CCGC 2 cut(s) 493, 908
AclWI GGATC 3 cut(s) 412, 530, 543
AcsI RAATTY 2 cut(s) 359, 682
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 2 cut(s) 29, 669
AfiI CCNNNNNNNGG 1 cut(s) 137
AgsI TTSAA 6 cut(s) 15, 211, 247, 256, 614, 643
AjiI CACGTC 1 cut(s) 192
AjnI CCWGG 2 cut(s) 236, 734
AluBI AGCT 5 cut(s) 286, 440, 458, 504, 516
AluI AGCT 5 cut(s) 286, 440, 458, 504, 516
Alw26I GTCTC 2 cut(s) 514, 714
AlwI GGATC 3 cut(s) 412, 530, 543
AlwNI CAGNNNCTG 1 cut(s) 867
Ama87I CYCGRG 1 cut(s) 111
ApeKI GCWGC 2 cut(s) 437, 455
ApoI RAATTY 2 cut(s) 359, 682
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 4 cut(s) 128, 634, 732, 851
AsuNHI GCTAGC 1 cut(s) 301
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 4 cut(s) 128, 634, 732, 851
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 449, 467
BccI CCATC 1 cut(s) 685
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 2 cut(s) 238, 736
BcoDI GTCTC 2 cut(s) 514, 714
BfaI CTAG 4 cut(s) 294, 302, 800, 932
BfmI CTRYAG 2 cut(s) 435, 868
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 2 cut(s) 438, 456
BlsI GCNGC 2 cut(s) 439, 457
Bme1390I CCNGG 2 cut(s) 238, 736
Bme18I GGWCC 4 cut(s) 128, 634, 732, 851
BmeT110I CYCGRG 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 192
BmgT120I GGNCC 4 cut(s) 128, 634, 732, 851
BmiI GGNNCC 6 cut(s) 129, 390, 537, 636, 797, 852
BmrFI CCNGG 2 cut(s) 238, 736
BmsI GCATC 2 cut(s) 504, 798
BmtI GCTAGC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 285
BpuEI CTTGAG 3 cut(s) 62, 538, 744
BsaJI CCNNGG 2 cut(s) 136, 237
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse3DI GCAATG 2 cut(s) 891, 924
BseBI CCWGG 2 cut(s) 238, 736
BseDI CCNNGG 2 cut(s) 136, 237
BseGI GGATG 3 cut(s) 523, 700, 813
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 2 cut(s) 891, 924
BseMII CTCAG 1 cut(s) 183
BseRI GAGGAG 2 cut(s) 129, 846
BseXI GCAGC 2 cut(s) 449, 467
Bsh1236I CGCG 1 cut(s) 918
BshNI GGYRCC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 1 cut(s) 827
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 2 cut(s) 514, 714
BsmFI GGGAC 1 cut(s) 620
BsoBI CYCGRG 1 cut(s) 111
Bsp1407I TGTACA 1 cut(s) 667
Bsp143I GATC 3 cut(s) 404, 535, 919
Bsp68I TCGCGA 1 cut(s) 918
BspACI CCGC 2 cut(s) 493, 908
BspCNI CTCAG 1 cut(s) 182
BspFNI CGCG 1 cut(s) 918
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 6 cut(s) 129, 390, 537, 636, 797, 852
BspMAI CTGCAG 2 cut(s) 439, 872
BspMI ACCTGC 1 cut(s) 442
BspOI GCTAGC 1 cut(s) 305
BspPI GGATC 3 cut(s) 412, 530, 543
BspT107I GGYRCC 1 cut(s) 388
BsrDI GCAATG 2 cut(s) 891, 924
BsrGI TGTACA 1 cut(s) 667
BssECI CCNNGG 2 cut(s) 136, 237
BssMI GATC 3 cut(s) 404, 535, 919
Bst2UI CCWGG 2 cut(s) 238, 736
Bst4CI ACNGT 3 cut(s) 415, 568, 732
BstAPI GCANNNNNTGC 2 cut(s) 473, 867
BstAUI TGTACA 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 108, 303
BstDEI CTNAG 1 cut(s) 169
BstDSI CCRYGG 1 cut(s) 136
BstEII GGTNACC 1 cut(s) 52
BstF5I GGATG 3 cut(s) 523, 700, 813
BstFNI CGCG 1 cut(s) 918
BstKTI GATC 3 cut(s) 407, 538, 922
BstMAI GTCTC 2 cut(s) 514, 714
BstMBI GATC 3 cut(s) 404, 535, 919
BstMWI GCNNNNNNNGC 5 cut(s) 92, 464, 473, 501, 867
BstNI CCWGG 2 cut(s) 238, 736
BstPI GGTNACC 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 236, 734
BstSFI CTRYAG 2 cut(s) 435, 868
BstUI CGCG 1 cut(s) 918
BstV1I GCAGC 2 cut(s) 449, 467
BstX2I RGATCY 2 cut(s) 404, 535
BstXI CCANNNNNNTGG 1 cut(s) 847
BstYI RGATCY 2 cut(s) 404, 535
BtgI CCRYGG 1 cut(s) 136
BtgZI GCGATG 1 cut(s) 899
BtrI CACGTC 1 cut(s) 192
BtsCI GGATG 3 cut(s) 523, 700, 813
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 1 cut(s) 868
BtuMI TCGCGA 1 cut(s) 918
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 108, 303
CaiI CAGNNNCTG 1 cut(s) 867
CciI TCATGA 1 cut(s) 498
Cfr13I GGNCC 4 cut(s) 128, 634, 732, 851
Csp6I GTAC 2 cut(s) 28, 668
CviAII CATG 4 cut(s) 424, 499, 592, 605
CviJI RGCY 9 cut(s) 47, 95, 286, 440, 458, 504, 516, 562, 764
CviKI_1 RGCY 9 cut(s) 47, 95, 286, 440, 458, 504, 516, 562, 764
CviQI GTAC 2 cut(s) 28, 668
DdeI CTNAG 1 cut(s) 169
DpnI GATC 3 cut(s) 406, 537, 921
DpnII GATC 3 cut(s) 404, 535, 919
EciI GGCGGA 1 cut(s) 897
Eco47I GGWCC 4 cut(s) 128, 634, 732, 851
Eco57I CTGAAG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 111
Eco91I GGTNACC 1 cut(s) 52
EcoO109I RGGNCCY 2 cut(s) 128, 851
EcoO65I GGTNACC 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 236, 734
EcoT22I ATGCAT 1 cut(s) 677
FaeI CATG 4 cut(s) 427, 502, 595, 608
FaqI GGGAC 1 cut(s) 620
FatI CATG 4 cut(s) 423, 498, 591, 604
FauI CCCGC 1 cut(s) 500
Fnu4HI GCNGC 2 cut(s) 438, 456
FokI GGATG 3 cut(s) 510, 707, 820
Fsp4HI GCNGC 2 cut(s) 438, 456
FspBI CTAG 4 cut(s) 294, 302, 800, 932
GluI GCNGC 2 cut(s) 438, 456
GsuI CTGGAG 1 cut(s) 285
HapII CCGG 1 cut(s) 827
Hin1II CATG 4 cut(s) 427, 502, 595, 608
HincII GTYRAC 2 cut(s) 589, 661
HindII GTYRAC 2 cut(s) 589, 661
HindIII AAGCTT 2 cut(s) 284, 514
HinfI GANTC 4 cut(s) 142, 614, 755, 783
HpaII CCGG 1 cut(s) 827
Hpy166II GTNNAC 4 cut(s) 121, 550, 589, 661
Hpy188I TCNGA 2 cut(s) 409, 572
Hpy188III TCNNGA 6 cut(s) 41, 224, 247, 499, 611, 917
Hpy8I GTNNAC 4 cut(s) 121, 550, 589, 661
Hpy99I CGWCG 1 cut(s) 193
HpyAV CCTTC 3 cut(s) 151, 538, 589
HpyCH4III ACNGT 3 cut(s) 415, 568, 732
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 5 cut(s) 281, 437, 467, 675, 870
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 464, 473, 501, 867
HpyF3I CTNAG 1 cut(s) 169
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 4 cut(s) 427, 502, 595, 608
Kzo9I GATC 3 cut(s) 404, 535, 919
LmnI GCTCC 2 cut(s) 266, 910
Lsp1109I GCAGC 2 cut(s) 449, 467
LweI GCATC 2 cut(s) 504, 798
MaeI CTAG 4 cut(s) 294, 302, 800, 932
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 4 cut(s) 52, 289, 863, 958
MalI GATC 3 cut(s) 406, 537, 921
MboI GATC 3 cut(s) 404, 535, 919
MboII GAAGA 2 cut(s) 3, 888
MflI RGATCY 2 cut(s) 404, 535
MluCI AATT 7 cut(s) 68, 101, 270, 359, 682, 816, 953
MmeI TCCRAC 1 cut(s) 550
Mph1103I ATGCAT 1 cut(s) 677
MseI TTAA 2 cut(s) 579, 627
MslI CAYNNNNRTG 2 cut(s) 629, 717
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 2 cut(s) 238, 736
MvaI CCWGG 2 cut(s) 238, 736
MvnI CGCG 1 cut(s) 918
MwoI GCNNNNNNNGC 5 cut(s) 92, 464, 473, 501, 867
NdeII GATC 3 cut(s) 404, 535, 919
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 4 cut(s) 427, 502, 595, 608
NlaIV GGNNCC 6 cut(s) 129, 390, 537, 636, 797, 852
NmuCI GTSAC 2 cut(s) 863, 958
NruI TCGCGA 1 cut(s) 918
NsiI ATGCAT 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 111
PagI TCATGA 1 cut(s) 498
PfeI GAWTC 4 cut(s) 142, 614, 755, 783
PfoI TCCNGGA 1 cut(s) 734
PkrI GCNGC 2 cut(s) 439, 457
PpuMI RGGWCCY 2 cut(s) 128, 851
PshBI ATTAAT 1 cut(s) 627
Psp5II RGGWCCY 2 cut(s) 128, 851
Psp6I CCWGG 2 cut(s) 236, 734
PspEI GGTNACC 1 cut(s) 52
PspGI CCWGG 2 cut(s) 236, 734
PspN4I GGNNCC 6 cut(s) 129, 390, 537, 636, 797, 852
PspPI GGNCC 4 cut(s) 128, 634, 732, 851
PspPPI RGGWCCY 2 cut(s) 128, 851
PspXI VCTCGAGB 1 cut(s) 111
PsrI GAACNNNNNNTAC 2 cut(s) 807, 839
PstI CTGCAG 2 cut(s) 439, 872
PstNI CAGNNNCTG 1 cut(s) 867
PsuI RGATCY 2 cut(s) 404, 535
RruI TCGCGA 1 cut(s) 918
RsaI GTAC 2 cut(s) 29, 669
RsaNI GTAC 2 cut(s) 28, 668
RseI CAYNNNNRTG 2 cut(s) 629, 717
SaqAI TTAA 2 cut(s) 579, 627
SatI GCNGC 2 cut(s) 438, 456
Sau3AI GATC 3 cut(s) 404, 535, 919
Sau96I GGNCC 4 cut(s) 128, 634, 732, 851
ScrFI CCNGG 2 cut(s) 238, 736
SfaNI GCATC 2 cut(s) 504, 798
SfcI CTRYAG 2 cut(s) 435, 868
Sfr274I CTCGAG 1 cut(s) 111
SinI GGWCC 4 cut(s) 128, 634, 732, 851
SlaI CTCGAG 1 cut(s) 111
SmiMI CAYNNNNRTG 2 cut(s) 629, 717
SmlI CTYRAG 4 cut(s) 41, 111, 517, 759
SmoI CTYRAG 4 cut(s) 41, 111, 517, 759
Sse9I AATT 7 cut(s) 68, 101, 270, 359, 682, 816, 953
SsiI CCGC 2 cut(s) 493, 908
SspI AATATT 2 cut(s) 352, 471
SspMI CTAG 4 cut(s) 294, 302, 800, 932
StyD4I CCNGG 2 cut(s) 236, 734
TaaI ACNGT 3 cut(s) 415, 568, 732
TaiI ACGT 1 cut(s) 194
TaqI TCGA 5 cut(s) 112, 231, 487, 531, 771
TasI AATT 7 cut(s) 68, 101, 270, 359, 682, 816, 953
TatI WGTACW 1 cut(s) 667
TfiI GAWTC 4 cut(s) 142, 614, 755, 783
Tru1I TTAA 2 cut(s) 579, 627
Tru9I TTAA 2 cut(s) 579, 627
TscAI CASTG 1 cut(s) 868
TseFI GTSAC 2 cut(s) 863, 958
TseI GCWGC 2 cut(s) 437, 455
Tsp45I GTSAC 2 cut(s) 863, 958
TspDTI ATGAA 6 cut(s) 35, 114, 363, 593, 608, 768
TspRI CASTG 1 cut(s) 868
VpaK11BI GGWCC 4 cut(s) 128, 634, 732, 851
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 2 cut(s) 359, 682
XhoI CTCGAG 1 cut(s) 111
XspI CTAG 4 cut(s) 294, 302, 800, 932
Zsp2I ATGCAT 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.