Rh7AG094300

glutamyl-tRNA(Gln) amidotransferase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004947
Physical Location & Seq
Forward (+)
7215929 .. 7218605
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG094300.1

Sequence Viewer

Length: 951 bp
ATGCAAAGATTGAAGAGGTACAATCCTGTTCTTGAGGCTGTGGTTACCAACATTGAAGAGTTAGCTAACCAGCAAGCAAAAGAGGCTGGTGAATTGCTCGCCCTAGGGGTGTATTTTGGTCCTCTCCATGGGATTCCTTATGGACTAAGGGATATAATATCAGTTCCCTACTACAAAACAACATGGGGTTCAAAAACTTATAAAGATCAAGTTCTTGACACGAAGGCCTGGGTTTATAAGAGGCTGAAGTCAGCAGGAGCAGTTCTCGTTGCCAAGCTTGCTTCTGGATCACTAGCTTATGATGACATCTGGTTTGGGGGTAGGACAAGGAACCCATGGAATATCAAGGAATTTACTACTGGTTCATCATCTGGACCCGCTGCTTGCACCTCAGCAGGTATGATACCATTTGCAATTGCATCAGAAACGGTTGGTTCCATGACTCTACCTGCTGCACACTGTGGGGTGACAGCATTGCGTCCAACATTTGGGTGTGTGGGTCGAACCGGTGTTATGAGTCTATCAGAAAGCTTGGATATGCTTGGTCCTGTCTCCAGAAGTGTCGCAGATTGTGCTATTATTTTGGATGCTATTAGAGGAAAGGATCCAGATGATCTTTCATCAAGAGACATTGCCCTTGAGGATCCTTCCCTGGTTGACATAACAAAGCTTACTATTGGATATGTCGATGATGCTGAGCAGGAGGTTGTTCATGTTCTTGGATCGAAAGGTGTTAATATGGTCCCTTTTAAACTTACATATGAGGTTGACTTAGTTGGTATTGTCAGCTTTACAATGGGCGTGGATACATTGGCTCACTTTGATGAGTGGCAACGGTCAGGGAAGGATGATGATTATGAATCGCAAGATAACGGCCTCCTGAGCTGCGTCGTGCACGCATTTTTCCAGCAGTGGACTATGTCCAGGCTCAGAGAGCAAGGGGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidase

Protein Analysis

316

Amino Acids

34.34

Weight (kDa)

5.11

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 2 - 235 3.8e-51 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 201, 237
Acc36I ACCTGC 2 cut(s) 386, 457
AccB7I CCANNNNNTGG 1 cut(s) 488
AciI CCGC 1 cut(s) 378
AclWI GGATC 6 cut(s) 295, 599, 612, 638, 651, 730
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 1 cut(s) 266
AdeI CACNNNGTG 1 cut(s) 461
AfaI GTAC 1 cut(s) 20
AfiI CCNNNNNNNGG 2 cut(s) 128, 488
AgeI ACCGGT 1 cut(s) 506
AgsI TTSAA 3 cut(s) 13, 56, 192
AjnI CCWGG 3 cut(s) 227, 651, 923
AluBI AGCT 8 cut(s) 65, 277, 296, 531, 670, 789, 885, 948
AluI AGCT 8 cut(s) 65, 277, 296, 531, 670, 789, 885, 948
Alw21I GWGCWC 1 cut(s) 897
Alw26I GTCTC 2 cut(s) 556, 621
Alw44I GTGCAC 1 cut(s) 893
AlwI GGATC 6 cut(s) 295, 599, 612, 638, 651, 730
AoxI GGCC 2 cut(s) 225, 874
ApaLI GTGCAC 1 cut(s) 893
ApeKI GCWGC 3 cut(s) 380, 452, 885
ApoI RAATTY 1 cut(s) 350
ArsI GACNNNNNNTTYG 2 cut(s) 296, 328
AsiGI ACCGGT 1 cut(s) 506
AspA2I CCTAGG 1 cut(s) 103
AspS9I GGNCC 4 cut(s) 119, 374, 545, 742
AsuHPI GGTGA 2 cut(s) 101, 478
AvaII GGWCC 4 cut(s) 119, 374, 545, 742
AvrII CCTAGG 1 cut(s) 103
BaeGI GKGCMC 1 cut(s) 897
BamHI GGATCC 2 cut(s) 604, 643
Bbv12I GWGCWC 1 cut(s) 897
BbvCI CCTCAGC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 367, 439, 872
BceAI ACGGC 1 cut(s) 889
BciT130I CCWGG 3 cut(s) 229, 653, 925
BciVI GTATCC 1 cut(s) 799
BcoDI GTCTC 2 cut(s) 556, 621
BfaI CTAG 2 cut(s) 104, 293
BfuAI ACCTGC 2 cut(s) 386, 457
BfuI GTATCC 1 cut(s) 799
BisI GCNGC 3 cut(s) 381, 453, 886
BlnI CCTAGG 1 cut(s) 103
BlpI GCTNAGC 1 cut(s) 696
BlsI GCNGC 3 cut(s) 382, 454, 887
Bme1390I CCNGG 3 cut(s) 229, 653, 925
Bme18I GGWCC 4 cut(s) 119, 374, 545, 742
BmgT120I GGNCC 4 cut(s) 119, 374, 545, 742
BmiI GGNNCC 6 cut(s) 332, 376, 436, 606, 645, 744
BmrFI CCNGG 3 cut(s) 229, 653, 925
BmsI GCATC 3 cut(s) 428, 577, 682
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpmI CTGGAG 1 cut(s) 538
Bpu10I CCTNAGC 2 cut(s) 391, 881
Bpu1102I GCTNAGC 1 cut(s) 696
BpuEI CTTGAG 2 cut(s) 53, 659
BsaJI CCNNGG 5 cut(s) 103, 127, 228, 335, 651
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 488
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 1 cut(s) 364
Bse3DI GCAATG 2 cut(s) 473, 630
BseBI CCWGG 3 cut(s) 229, 653, 925
BseDI CCNNGG 5 cut(s) 103, 127, 228, 335, 651
BseGI GGATG 2 cut(s) 592, 853
BseLI CCNNNNNNNGG 2 cut(s) 128, 488
BseMI GCAATG 2 cut(s) 473, 630
BseMII CTCAG 4 cut(s) 405, 687, 872, 943
BseNI ACTGG 1 cut(s) 364
BseSI GKGCMC 1 cut(s) 897
BseXI GCAGC 3 cut(s) 367, 439, 872
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 2 cut(s) 227, 876
BshTI ACCGGT 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 897
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 2 cut(s) 128, 488
BsmAI GTCTC 2 cut(s) 556, 621
BsmFI GGGAC 1 cut(s) 728
BsnI GGCC 2 cut(s) 227, 876
Bsp1286I GDGCHC 1 cut(s) 897
Bsp143I GATC 6 cut(s) 205, 287, 604, 613, 643, 722
Bsp1720I GCTNAGC 1 cut(s) 696
Bsp19I CCATGG 2 cut(s) 127, 335
BspACI CCGC 1 cut(s) 378
BspANI GGCC 2 cut(s) 227, 876
BspCNI CTCAG 4 cut(s) 404, 688, 873, 942
BspLI GGNNCC 6 cut(s) 332, 376, 436, 606, 645, 744
BspMI ACCTGC 2 cut(s) 386, 457
BspPI GGATC 6 cut(s) 295, 599, 612, 638, 651, 730
BsrDI GCAATG 2 cut(s) 473, 630
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 1 cut(s) 364
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 5 cut(s) 103, 127, 228, 335, 651
BssMI GATC 6 cut(s) 205, 287, 604, 613, 643, 722
BssT1I CCWWGG 3 cut(s) 103, 127, 335
Bst2UI CCWGG 3 cut(s) 229, 653, 925
Bst4CI ACNGT 3 cut(s) 430, 461, 837
Bst6I CTCTTC 2 cut(s) 8, 51
BstAPI GCANNNNNTGC 1 cut(s) 572
BstC8I GCNNGC 5 cut(s) 75, 99, 279, 385, 897
BstDEI CTNAG 6 cut(s) 146, 391, 696, 772, 881, 929
BstDSI CCRYGG 2 cut(s) 127, 335
BstEII GGTNACC 1 cut(s) 43
BstF5I GGATG 2 cut(s) 592, 853
BstKTI GATC 6 cut(s) 208, 290, 607, 616, 646, 725
BstMAI GTCTC 2 cut(s) 556, 621
BstMBI GATC 6 cut(s) 205, 287, 604, 613, 643, 722
BstMWI GCNNNNNNNGC 5 cut(s) 83, 278, 572, 882, 934
BstNI CCWGG 3 cut(s) 229, 653, 925
BstPI GGTNACC 1 cut(s) 43
BstSCI CCNGG 3 cut(s) 227, 651, 923
BstSLI GKGCMC 1 cut(s) 897
BstV1I GCAGC 3 cut(s) 367, 439, 872
BstX2I RGATCY 2 cut(s) 604, 643
BstYI RGATCY 2 cut(s) 604, 643
BsuI GTATCC 1 cut(s) 799
BsuRI GGCC 2 cut(s) 227, 876
BtgI CCRYGG 2 cut(s) 127, 335
BtsCI GGATG 2 cut(s) 592, 853
BtsI GCAGTG 1 cut(s) 917
BtsIMutI CAGTG 2 cut(s) 457, 917
BveI ACCTGC 2 cut(s) 386, 457
Cac8I GCNNGC 5 cut(s) 75, 99, 279, 385, 897
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 4 cut(s) 119, 374, 545, 742
CseI GACGC 2 cut(s) 467, 877
Csp6I GTAC 1 cut(s) 19
CspAI ACCGGT 1 cut(s) 506
CspCI CAANNNNNGTGG 2 cut(s) 783, 818
CviAII CATG 5 cut(s) 128, 183, 336, 439, 713
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 6 cut(s) 146, 391, 696, 772, 881, 929
DpnI GATC 6 cut(s) 207, 289, 606, 615, 645, 724
DpnII GATC 6 cut(s) 205, 287, 604, 613, 643, 722
DraI TTTAAA 1 cut(s) 751
DraIII CACNNNGTG 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 8, 51
EarI CTCTTC 2 cut(s) 8, 51
Eco130I CCWWGG 3 cut(s) 103, 127, 335
Eco147I AGGCCT 1 cut(s) 227
Eco47I GGWCC 4 cut(s) 119, 374, 545, 742
Eco57I CTGAAG 1 cut(s) 266
Eco91I GGTNACC 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 43
EcoRII CCWGG 3 cut(s) 227, 651, 923
EcoT14I CCWWGG 3 cut(s) 103, 127, 335
ErhI CCWWGG 3 cut(s) 103, 127, 335
FaeI CATG 5 cut(s) 131, 186, 339, 442, 716
FaqI GGGAC 1 cut(s) 728
FatI CATG 5 cut(s) 127, 182, 335, 438, 712
FauI CCCGC 1 cut(s) 385
FauNDI CATATG 1 cut(s) 760
Fnu4HI GCNGC 3 cut(s) 381, 453, 886
FokI GGATG 2 cut(s) 599, 860
Fsp4HI GCNGC 3 cut(s) 381, 453, 886
FspBI CTAG 2 cut(s) 104, 293
GluI GCNGC 3 cut(s) 381, 453, 886
GsuI CTGGAG 1 cut(s) 538
HaeIII GGCC 2 cut(s) 227, 876
HapII CCGG 1 cut(s) 507
HgaI GACGC 2 cut(s) 467, 877
Hin1II CATG 5 cut(s) 131, 186, 339, 442, 716
HincII GTYRAC 2 cut(s) 658, 769
HindII GTYRAC 2 cut(s) 658, 769
HindIII AAGCTT 3 cut(s) 275, 529, 668
HinfI GANTC 4 cut(s) 133, 442, 517, 860
HpaII CCGG 1 cut(s) 507
HphI GGTGA 2 cut(s) 101, 478
Hpy166II GTNNAC 4 cut(s) 658, 769, 895, 915
Hpy188I TCNGA 3 cut(s) 424, 526, 932
Hpy188III TCNNGA 8 cut(s) 32, 215, 285, 372, 555, 608, 624, 880
Hpy8I GTNNAC 4 cut(s) 658, 769, 895, 915
Hpy99I CGWCG 1 cut(s) 893
HpyAV CCTTC 3 cut(s) 217, 657, 838
HpyCH4III ACNGT 3 cut(s) 430, 461, 837
HpyCH4V TGCA 6 cut(s) 4, 387, 413, 419, 455, 895
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 278, 572, 882, 934
HpyF3I CTNAG 6 cut(s) 146, 391, 696, 772, 881, 929
Hsp92II CATG 5 cut(s) 131, 186, 339, 442, 716
Kzo9I GATC 6 cut(s) 205, 287, 604, 613, 643, 722
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 3 cut(s) 367, 439, 872
LweI GCATC 3 cut(s) 428, 577, 682
MaeI CTAG 2 cut(s) 104, 293
MaeIII GTNAC 2 cut(s) 43, 466
MalI GATC 6 cut(s) 207, 289, 606, 615, 645, 724
MboI GATC 6 cut(s) 205, 287, 604, 613, 643, 722
MboII GAAGA 2 cut(s) 25, 68
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 2 cut(s) 604, 643
MhlI GDGCHC 1 cut(s) 897
MluCI AATT 3 cut(s) 92, 350, 414
MlyI GAGTC 2 cut(s) 436, 526
MmeI TCCRAC 1 cut(s) 506
MseI TTAA 2 cut(s) 735, 750
MslI CAYNNNNRTG 2 cut(s) 490, 822
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 3 cut(s) 229, 653, 925
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 3 cut(s) 229, 653, 925
MwoI GCNNNNNNNGC 5 cut(s) 83, 278, 572, 882, 934
NcoI CCATGG 2 cut(s) 127, 335
NdeI CATATG 1 cut(s) 760
NdeII GATC 6 cut(s) 205, 287, 604, 613, 643, 722
NlaIII CATG 5 cut(s) 131, 186, 339, 442, 716
NlaIV GGNNCC 6 cut(s) 332, 376, 436, 606, 645, 744
NmuCI GTSAC 1 cut(s) 466
PceI AGGCCT 1 cut(s) 227
PfeI GAWTC 2 cut(s) 133, 860
PflFI GACNNNGTC 1 cut(s) 919
PflMI CCANNNNNTGG 1 cut(s) 488
PinAI ACCGGT 1 cut(s) 506
PkrI GCNGC 3 cut(s) 382, 454, 887
PleI GAGTC 2 cut(s) 436, 525
PpsI GAGTC 2 cut(s) 436, 525
PsiI TTATAA 2 cut(s) 201, 237
Psp6I CCWGG 3 cut(s) 227, 651, 923
PspEI GGTNACC 1 cut(s) 43
PspGI CCWGG 3 cut(s) 227, 651, 923
PspN4I GGNNCC 6 cut(s) 332, 376, 436, 606, 645, 744
PspPI GGNCC 4 cut(s) 119, 374, 545, 742
PsuI RGATCY 2 cut(s) 604, 643
PsyI GACNNNGTC 1 cut(s) 919
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
RseI CAYNNNNRTG 2 cut(s) 490, 822
SaqAI TTAA 2 cut(s) 735, 750
SatI GCNGC 3 cut(s) 381, 453, 886
Sau3AI GATC 6 cut(s) 205, 287, 604, 613, 643, 722
Sau96I GGNCC 4 cut(s) 119, 374, 545, 742
SchI GAGTC 2 cut(s) 436, 526
ScrFI CCNGG 3 cut(s) 229, 653, 925
SduI GDGCHC 1 cut(s) 897
SfaNI GCATC 3 cut(s) 428, 577, 682
SinI GGWCC 4 cut(s) 119, 374, 545, 742
SmiMI CAYNNNNRTG 2 cut(s) 490, 822
SmlI CTYRAG 2 cut(s) 32, 638
SmoI CTYRAG 2 cut(s) 32, 638
Sse9I AATT 3 cut(s) 92, 350, 414
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 1 cut(s) 378
SspMI CTAG 2 cut(s) 104, 293
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 3 cut(s) 227, 651, 923
StyI CCWWGG 3 cut(s) 103, 127, 335
TaaI ACNGT 3 cut(s) 430, 461, 837
TaqI TCGA 3 cut(s) 502, 687, 725
TasI AATT 3 cut(s) 92, 350, 414
TfiI GAWTC 2 cut(s) 133, 860
Tru1I TTAA 2 cut(s) 735, 750
Tru9I TTAA 2 cut(s) 735, 750
TscAI CASTG 2 cut(s) 464, 917
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 3 cut(s) 380, 452, 885
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 4 cut(s) 354, 609, 701, 873
TspRI CASTG 2 cut(s) 464, 917
Tth111I GACNNNGTC 1 cut(s) 919
Van91I CCANNNNNTGG 1 cut(s) 488
VneI GTGCAC 1 cut(s) 893
VpaK11BI GGWCC 4 cut(s) 119, 374, 545, 742
XapI RAATTY 1 cut(s) 350
XmaJI CCTAGG 1 cut(s) 103
XspI CTAG 2 cut(s) 104, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.