RchiOBHm_Chr7g0188311

glutamyl-tRNA(Gln) amidotransferase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004947
Physical Location & Seq
Forward (+)
7898384 .. 7900749
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16816

Sequence Viewer

Length: 1038 bp
ATGAGTGTTCTTGAATTGGGGGAGCTCATAAGGACAAAGCAAATTACATCCCAGGAGCTTACTCAGATTTTTATGCAAAGATTGAAGAGGCTGAAGTCAGCAGGAGCAGTTCTCGTTGCCAAGCTTGCTTCTGGATCACTAGCTTATGATGACATCTGGTTTGGGGGTAGGACAAGGAACCCATGGAATATCAAGGAATTTACTACTGGTTCATCATCTGGACCCGCTGCTTGCACCTCAGCAGGTATGATAAAATTTGCAATTGCATCAGAAACGGTTGGTTCCATGACTCTACCTGCTGCACACTGTGGGGTGACAGCATTGCGTCCAACATTTGGGTGTGTGGGTCGAACCGGTGTTATGAGTCTATCAGAAAGCTTGGATAAGCTTGGTCCTGTCTCCAGAAGTGTCGCAGATTGTGCTATTATTTTGGATGCTATTAGAGGAAAGGATCCAGATGATCTTTCATCAAGAGACATTGCCCTTGAGGATCCTTCCCTGGTTGACATAACAAAGCTTACTATTGGATATGTCGATGATGCTGAGCAGGAGGTTGTTCATGTTCTTGGATCGAAAGGTGTTAATATGGTCCCTTTTAAACTTAAATATGAGGTTGACTTAGTTGGTATTGTCAGCTTTACAATGGGCGTGGATACATTGGCTCACTTTGATGAGTGGCAACGGTCAGGGAAGGATGATGATTATGAATCGCAAGATAATGGCCTCCTGAGCTGCGTCGTGCACGCATTTTTCCAGCAGTGGACTATGTCCAGGTTGGCTCAGAGAGCAAGGGGGAAGCTGATTCTAGAAGTAAGAGAGAGCTTCACTGTTGACGCATTCATTGGCAATGCAGCTGATTGGGAGAGAGTTTGTATGGGAAACCTGGTAGGGATGCCTGTAATCGTTGTTCCATCGGGGTTCCCCAAGATATCTAACCCACCTTGCAATGATTGTCGTCGAAGAGCCACAATCACCACTGGTGTTAGAACTAAATGGACCTATCATCATCTATTATACAGAATGCAAATTGGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidase

Protein Analysis

345

Amino Acids

37.74

Weight (kDa)

6.6

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 29 - 188 4.9e-34 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1036
Acc36I ACCTGC 2 cut(s) 233, 304
AccB7I CCANNNNNTGG 1 cut(s) 335
AciI CCGC 1 cut(s) 225
AclWI GGATC 6 cut(s) 142, 446, 459, 485, 498, 577
AcsI RAATTY 2 cut(s) 197, 254
AcuI CTGAAG 1 cut(s) 113
AdeI CACNNNGTG 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 335
AgeI ACCGGT 1 cut(s) 353
AgsI TTSAA 2 cut(s) 14, 85
AjnI CCWGG 4 cut(s) 51, 498, 770, 882
Alw21I GWGCWC 2 cut(s) 27, 744
Alw26I GTCTC 2 cut(s) 403, 468
Alw44I GTGCAC 1 cut(s) 740
AlwI GGATC 6 cut(s) 142, 446, 459, 485, 498, 577
AoxI GGCC 1 cut(s) 721
ApaLI GTGCAC 1 cut(s) 740
ApeKI GCWGC 4 cut(s) 227, 299, 732, 851
ApoI RAATTY 2 cut(s) 197, 254
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
AsiGI ACCGGT 1 cut(s) 353
AspS9I GGNCC 4 cut(s) 221, 392, 589, 996
AsuHPI GGTGA 2 cut(s) 325, 964
AvaII GGWCC 4 cut(s) 221, 392, 589, 996
BaeGI GKGCMC 1 cut(s) 744
BamHI GGATCC 2 cut(s) 451, 490
BanII GRGCYC 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 27, 744
BbvCI CCTCAGC 1 cut(s) 238
BbvI GCAGC 4 cut(s) 214, 286, 719, 863
BccI CCATC 1 cut(s) 919
BciT130I CCWGG 4 cut(s) 53, 500, 772, 884
BciVI GTATCC 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 403, 468
BfaI CTAG 2 cut(s) 140, 806
BfuAI ACCTGC 2 cut(s) 233, 304
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 4 cut(s) 228, 300, 733, 852
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 4 cut(s) 229, 301, 734, 853
Bme1390I CCNGG 4 cut(s) 53, 500, 772, 884
Bme18I GGWCC 4 cut(s) 221, 392, 589, 996
BmgT120I GGNCC 4 cut(s) 221, 392, 589, 996
BmiI GGNNCC 7 cut(s) 179, 223, 283, 453, 492, 591, 920
BmrFI CCNGG 4 cut(s) 53, 500, 772, 884
BmsI GCATC 4 cut(s) 275, 424, 529, 882
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpmI CTGGAG 1 cut(s) 385
Bpu10I CCTNAGC 2 cut(s) 238, 728
Bpu1102I GCTNAGC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 51, 182, 498
BsaWI WCCGGW 1 cut(s) 353
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 2 cut(s) 211, 982
Bse3DI GCAATG 4 cut(s) 320, 477, 853, 952
BseBI CCWGG 4 cut(s) 53, 500, 772, 884
BseDI CCNNGG 3 cut(s) 51, 182, 498
BseGI GGATG 4 cut(s) 47, 439, 700, 897
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 4 cut(s) 320, 477, 853, 952
BseMII CTCAG 5 cut(s) 77, 252, 534, 719, 794
BseNI ACTGG 2 cut(s) 211, 982
BseSI GKGCMC 1 cut(s) 744
BseXI GCAGC 4 cut(s) 214, 286, 719, 863
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 1 cut(s) 723
BshTI ACCGGT 1 cut(s) 353
BsiHKAI GWGCWC 2 cut(s) 27, 744
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 2 cut(s) 403, 468
BsmFI GGGAC 1 cut(s) 575
BsmI GAATGC 2 cut(s) 836, 1026
BsnI GGCC 1 cut(s) 723
Bsp1286I GDGCHC 2 cut(s) 27, 744
Bsp143I GATC 5 cut(s) 134, 451, 460, 490, 569
Bsp1720I GCTNAGC 1 cut(s) 543
Bsp19I CCATGG 1 cut(s) 182
BspACI CCGC 1 cut(s) 225
BspANI GGCC 1 cut(s) 723
BspCNI CTCAG 5 cut(s) 76, 251, 535, 720, 793
BspLI GGNNCC 7 cut(s) 179, 223, 283, 453, 492, 591, 920
BspMI ACCTGC 2 cut(s) 233, 304
BspPI GGATC 6 cut(s) 142, 446, 459, 485, 498, 577
BspQI GCTCTTC 1 cut(s) 955
BsrDI GCAATG 4 cut(s) 320, 477, 853, 952
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 2 cut(s) 211, 982
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 3 cut(s) 51, 182, 498
BssMI GATC 5 cut(s) 134, 451, 460, 490, 569
BssT1I CCWWGG 1 cut(s) 182
Bst2UI CCWGG 4 cut(s) 53, 500, 772, 884
Bst4CI ACNGT 4 cut(s) 277, 308, 684, 829
Bst6I CTCTTC 2 cut(s) 80, 955
BstAPI GCANNNNNTGC 1 cut(s) 419
BstC8I GCNNGC 3 cut(s) 126, 232, 744
BstDEI CTNAG 6 cut(s) 63, 238, 543, 619, 728, 780
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 4 cut(s) 47, 439, 700, 897
BstKTI GATC 5 cut(s) 137, 454, 463, 493, 572
BstMAI GTCTC 2 cut(s) 403, 468
BstMBI GATC 5 cut(s) 134, 451, 460, 490, 569
BstMWI GCNNNNNNNGC 4 cut(s) 125, 419, 729, 785
BstNI CCWGG 4 cut(s) 53, 500, 772, 884
BstSCI CCNGG 4 cut(s) 51, 498, 770, 882
BstSLI GKGCMC 1 cut(s) 744
BstV1I GCAGC 4 cut(s) 214, 286, 719, 863
BstX2I RGATCY 2 cut(s) 451, 490
BstYI RGATCY 2 cut(s) 451, 490
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 182
BtsCI GGATG 4 cut(s) 47, 439, 700, 897
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 4 cut(s) 304, 764, 825, 975
BveI ACCTGC 2 cut(s) 233, 304
Cac8I GCNNGC 3 cut(s) 126, 232, 744
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 4 cut(s) 221, 392, 589, 996
CseI GACGC 3 cut(s) 314, 724, 842
CsiI ACCWGGT 1 cut(s) 882
CspAI ACCGGT 1 cut(s) 353
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CviAII CATG 3 cut(s) 183, 286, 560
DdeI CTNAG 6 cut(s) 63, 238, 543, 619, 728, 780
DpnI GATC 5 cut(s) 136, 453, 462, 492, 571
DpnII GATC 5 cut(s) 134, 451, 460, 490, 569
DraI TTTAAA 1 cut(s) 598
DraIII CACNNNGTG 1 cut(s) 308
Eam1104I CTCTTC 2 cut(s) 80, 955
EarI CTCTTC 2 cut(s) 80, 955
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 1 cut(s) 930
Eco47I GGWCC 4 cut(s) 221, 392, 589, 996
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 25
EcoRII CCWGG 4 cut(s) 51, 498, 770, 882
EcoRV GATATC 1 cut(s) 930
EcoT14I CCWWGG 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 3 cut(s) 186, 289, 563
FaqI GGGAC 1 cut(s) 575
FatI CATG 3 cut(s) 182, 285, 559
FauI CCCGC 1 cut(s) 232
Fnu4HI GCNGC 4 cut(s) 228, 300, 733, 852
FokI GGATG 4 cut(s) 34, 446, 707, 904
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 4 cut(s) 228, 300, 733, 852
FspBI CTAG 2 cut(s) 140, 806
GluI GCNGC 4 cut(s) 228, 300, 733, 852
GsuI CTGGAG 1 cut(s) 385
HaeIII GGCC 1 cut(s) 723
HapII CCGG 1 cut(s) 354
HgaI GACGC 3 cut(s) 314, 724, 842
Hin1II CATG 3 cut(s) 186, 289, 563
HincII GTYRAC 3 cut(s) 505, 616, 832
HindII GTYRAC 3 cut(s) 505, 616, 832
HindIII AAGCTT 4 cut(s) 122, 376, 386, 515
HinfI GANTC 4 cut(s) 289, 364, 707, 802
HpaII CCGG 1 cut(s) 354
HphI GGTGA 2 cut(s) 325, 964
Hpy166II GTNNAC 5 cut(s) 505, 616, 742, 762, 832
Hpy188I TCNGA 4 cut(s) 66, 271, 373, 783
Hpy188III TCNNGA 8 cut(s) 11, 132, 219, 402, 455, 471, 727, 806
Hpy8I GTNNAC 5 cut(s) 505, 616, 742, 762, 832
Hpy99I CGWCG 2 cut(s) 740, 960
HpyAV CCTTC 2 cut(s) 504, 685
HpyCH4III ACNGT 4 cut(s) 277, 308, 684, 829
HpyCH4V TGCA 9 cut(s) 76, 234, 260, 266, 302, 742, 851, 945, 1024
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 419, 729, 785
HpyF3I CTNAG 6 cut(s) 63, 238, 543, 619, 728, 780
Hsp92II CATG 3 cut(s) 186, 289, 563
Kzo9I GATC 5 cut(s) 134, 451, 460, 490, 569
LguI GCTCTTC 1 cut(s) 955
LmnI GCTCC 3 cut(s) 22, 55, 104
Lsp1109I GCAGC 4 cut(s) 214, 286, 719, 863
LweI GCATC 4 cut(s) 275, 424, 529, 882
MabI ACCWGGT 1 cut(s) 882
MaeI CTAG 2 cut(s) 140, 806
MaeIII GTNAC 1 cut(s) 313
MalI GATC 5 cut(s) 136, 453, 462, 492, 571
MboI GATC 5 cut(s) 134, 451, 460, 490, 569
MboII GAAGA 2 cut(s) 97, 972
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 2 cut(s) 451, 490
MhlI GDGCHC 2 cut(s) 27, 744
MluCI AATT 6 cut(s) 14, 42, 197, 254, 261, 1026
MlyI GAGTC 2 cut(s) 283, 373
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 7 cut(s) 81, 247, 437, 481, 544, 604, 734
MseI TTAA 3 cut(s) 582, 597, 603
MslI CAYNNNNRTG 2 cut(s) 337, 669
MspA1I CMGCKG 2 cut(s) 227, 854
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 4 cut(s) 53, 500, 772, 884
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 2 cut(s) 836, 1026
MvaI CCWGG 4 cut(s) 53, 500, 772, 884
MwoI GCNNNNNNNGC 4 cut(s) 125, 419, 729, 785
NcoI CCATGG 1 cut(s) 182
NdeII GATC 5 cut(s) 134, 451, 460, 490, 569
NlaIII CATG 3 cut(s) 186, 289, 563
NlaIV GGNNCC 7 cut(s) 179, 223, 283, 453, 492, 591, 920
NmuCI GTSAC 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 955
PctI GAATGC 2 cut(s) 836, 1026
PfeI GAWTC 2 cut(s) 707, 802
PflFI GACNNNGTC 1 cut(s) 766
PflMI CCANNNNNTGG 1 cut(s) 335
PinAI ACCGGT 1 cut(s) 353
PkrI GCNGC 4 cut(s) 229, 301, 734, 853
PleI GAGTC 2 cut(s) 283, 372
PpsI GAGTC 2 cut(s) 283, 372
PsiI TTATAA 1 cut(s) 1036
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 4 cut(s) 51, 498, 770, 882
PspGI CCWGG 4 cut(s) 51, 498, 770, 882
PspN4I GGNNCC 7 cut(s) 179, 223, 283, 453, 492, 591, 920
PspPI GGNCC 4 cut(s) 221, 392, 589, 996
PsrI GAACNNNNNNTAC 2 cut(s) 891, 923
PsuI RGATCY 2 cut(s) 451, 490
PsyI GACNNNGTC 1 cut(s) 766
PvuII CAGCTG 1 cut(s) 854
RseI CAYNNNNRTG 2 cut(s) 337, 669
SacI GAGCTC 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 955
SaqAI TTAA 3 cut(s) 582, 597, 603
SatI GCNGC 4 cut(s) 228, 300, 733, 852
Sau3AI GATC 5 cut(s) 134, 451, 460, 490, 569
Sau96I GGNCC 4 cut(s) 221, 392, 589, 996
SchI GAGTC 2 cut(s) 283, 373
ScrFI CCNGG 4 cut(s) 53, 500, 772, 884
SduI GDGCHC 2 cut(s) 27, 744
SexAI ACCWGGT 1 cut(s) 882
SfaNI GCATC 4 cut(s) 275, 424, 529, 882
SinI GGWCC 4 cut(s) 221, 392, 589, 996
SmiMI CAYNNNNRTG 2 cut(s) 337, 669
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 6 cut(s) 14, 42, 197, 254, 261, 1026
SsiI CCGC 1 cut(s) 225
SspMI CTAG 2 cut(s) 140, 806
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 4 cut(s) 51, 498, 770, 882
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 4 cut(s) 277, 308, 684, 829
TaqI TCGA 4 cut(s) 349, 534, 572, 958
TasI AATT 6 cut(s) 14, 42, 197, 254, 261, 1026
TfiI GAWTC 2 cut(s) 707, 802
Tru1I TTAA 3 cut(s) 582, 597, 603
Tru9I TTAA 3 cut(s) 582, 597, 603
TscAI CASTG 4 cut(s) 311, 764, 832, 982
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 4 cut(s) 227, 299, 732, 851
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 201, 456, 548, 720, 829
TspRI CASTG 4 cut(s) 311, 764, 832, 982
Tth111I GACNNNGTC 1 cut(s) 766
Van91I CCANNNNNTGG 1 cut(s) 335
VneI GTGCAC 1 cut(s) 740
VpaK11BI GGWCC 4 cut(s) 221, 392, 589, 996
XapI RAATTY 2 cut(s) 197, 254
XbaI TCTAGA 1 cut(s) 805
XspI CTAG 2 cut(s) 140, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.