AT5G08305

pentatricopeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016589
Physical Location & Seq
Reverse (-)
2669944 .. 2671782
1839 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08305.1

Sequence Viewer

Length: 1605 bp
ATGTTGAAATCATCATCGTCTCTCGTTGCGAAATCGATTCTGCGTCACCAATGCAAATCAATGTCGGAGCTGTACAAAATACACACCCTCCTGATCACACTTGGTCTCTCCGAGGAAGAGCCTTTTGTTTCTCAAACTCTGTCTTTCTCTGCTTTGTCTTCATCAGGCGACGTTGACTACGCTTACAAATTCTTGTCAAAACTATCTGATCCTCCGAATTACGGATGGAATTTCGTAATACGAGGTTTCTCCAATAGCAGAAACCCCGAGAAATCGATCAGCGTCTATATCCAGATGTTGAGGTTCGGGTTATTACCTGATCATATGACTTATCCGTTTCTTATGAAGTCGAGTTCTCGTCTCTCGAACAGAAAACTTGGTGGGTCTTTGCATTGCTCCGTGGTTAAATCTGGGCTTGAGTGGGATTTGTTCATATGTAATACTCTTATTCATATGTATGGTTCGTTCCGAGACCAAGCTTCTGCGCGCAAGTTGTTCGATGAAATGCCTCATAAGAACTTGGTTACTTGGAACTCTATTTTAGATGCGTATGCCAAGAGCGGGGATGTTGTTTCAGCAAGACTGGTGTTTGATGAAATGAGTGAGAGAGATGTTGTGACTTGGAGTTCTATGATCGATGGCTATGTAAAAAGAGGTGAATATAACAAAGCGTTGGAAATTTTCGATCAGATGATGAGAATGGGTTCATCAAAGGCAAATGAAGTCACTATGGTTAGCGTTATATGCGCTTGTGCTCACTTGGGTGCGCTAAATCGAGGAAAGACTGTACATCGCTATATACTCGATGTGCATTTGCCGTTAACAGTTATATTGCAGACATCTCTTATTGATATGTATGCGAAATGCGGATCGATAGGAGACGCTTGGAGCGTGTTTTATAGAGCATCTGTTAAGGAAACAGATGCCTTGATGTGGAATGCTATCATTGGAGGGCTTGCGAGTCACGGGTTCATTAGAGAGTCGCTCCAACTGTTTCACAAAATGCGAGAATCAAAGATTGACCCGGACGAGATAACATTCTTGTGCTTGCTTGCTGCTTGTTCCCATGGCGGATTAGTGAAAGAAGCGTGGCATTTCTTCAAGAGTCTCAAGGAAAGTGGTGCGGAACCAAAGAGCGAGCATTATGCTTGTATGGTTGATGTGCTTTCCAGAGCTGGTCTAGTTAAAGACGCACATGATTTTATATCTGAAATGCCGATAAAACCGACGGGTTCAATGTTGGGTGCTTTGCTTAACGGGTGTATAAATCACGGAAACTTGGAGCTTGCGGAAACAGTTGGAAAGAAGCTTATAGAGTTGCAACCACATAATGATGGACGATATGTCGGGCTAGCAAACGTTTATGCGATCAACAAGCAGTTTAGGGCAGCTCGGTCTATGAGAGAAGCAATGGAGAAGAAAGGAGTGAAGAAAATTGCAGGGCACAGTATACTTGATTTGGATGGAACACGACATAGATTCATCGCTCATGACAAAACACATTTTCACTCCGACAAGATCTATGCAGTGTTACAGCTGACTGGAGCTTGGATGAATCTCGATGTTGACTATGATGATCAAGACAATCATTGTTTTTGTTCTTGA

Protein Analysis

534

Amino Acids

59.92

Weight (kDa)

7.92

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 173 - 204 4.3e-06 PPR repeat family
PPR PF01535 175 - 204 5.1e-07 PPR repeat
PPR_2 PF13041 203 - 253 1.8e-10 PPR repeat family
PPR_1 PF12854 203 - 231 5.9e-07 PPR repeat
PPR PF01535 206 - 235 2.1e-09 PPR repeat
PPR_2 PF13041 308 - 355 9.9e-09 PPR repeat family
E_motif PF20431 424 - 484 3.4e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016589)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08305
fragaria_vesca FvH4_3g24460
pyrus_communis pycom03g09570
rosa_chinensis RchiOBHm_Chr5g0043961
rosa_laevigata RLG00000034228
rosa_multiflora Rmu_co8265095.1_g000001 Rmu_sc0002988.1_g000009 Rmu_sc0006669.1_g000009
rosa_roxburghii Rroxscaffold_1G00037100
rosa_rugosa Rorug05G0209400
rosa_samantha Rh5AG293800 Rh5BG302000 Rh5CG329200
rosa_wichuraiana Rw5G027220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 561
AccI GTMKAC 1 cut(s) 1450
AccII CGCG 1 cut(s) 487
AciI CCGC 5 cut(s) 561, 867, 1071, 1124, 1289
AclI AACGTT 1 cut(s) 1359
AclWI GGATC 2 cut(s) 203, 877
AcsI RAATTY 3 cut(s) 188, 229, 678
AfaI GTAC 2 cut(s) 74, 789
AfiI CCNNNNNNNGG 3 cut(s) 221, 561, 933
AgsI TTSAA 3 cut(s) 7, 1102, 1236
AhdI GACNNNNNGTC 1 cut(s) 1343
AleI CACNNNNGTG 1 cut(s) 762
AluBI AGCT 8 cut(s) 70, 479, 1175, 1285, 1309, 1391, 1537, 1547
AluI AGCT 8 cut(s) 70, 479, 1175, 1285, 1309, 1391, 1537, 1547
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 6 cut(s) 24, 110, 365, 465, 873, 1112
AlwI GGATC 2 cut(s) 203, 877
Ama87I CYCGRG 1 cut(s) 266
ApeKI GCWGC 2 cut(s) 1055, 1388
ApoI RAATTY 3 cut(s) 188, 229, 678
Asp700I GAANNNNTTC 1 cut(s) 703
AspLEI GCGC 4 cut(s) 487, 489, 749, 769
AsuC2I CCSGG 1 cut(s) 1025
AsuHPI GGTGA 2 cut(s) 38, 668
AsuNHI GCTAGC 1 cut(s) 1351
AvaI CYCGRG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 1446
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 2 cut(s) 1042, 1400
BccI CCATC 4 cut(s) 219, 632, 1328, 1457
BceAI ACGGC 1 cut(s) 802
BcgI CGANNNNNNTGC 4 cut(s) 478, 512, 1127, 1161
BclI TGATCA 3 cut(s) 93, 319, 1576
BcnI CCSGG 1 cut(s) 1025
BcoDI GTCTC 6 cut(s) 24, 110, 365, 465, 873, 1112
BfaI CTAG 2 cut(s) 1181, 1352
BglII AGATCT 1 cut(s) 1518
BisI GCNGC 2 cut(s) 1056, 1389
BlsI GCNGC 2 cut(s) 1057, 1390
Bme1390I CCNGG 1 cut(s) 1025
BmeRI GACNNNNNGTC 1 cut(s) 1343
BmeT110I CYCGRG 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 1128
BmrFI CCNGG 1 cut(s) 1025
BmsI GCATC 3 cut(s) 535, 913, 914
BmtI GCTAGC 1 cut(s) 1355
BpiI GAAGAC 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 969, 1001
BpmI CTGGAG 1 cut(s) 1563
BpuEI CTTGAG 2 cut(s) 437, 1094
BpuMI CCSGG 1 cut(s) 1025
Bsa29I ATCGAT 4 cut(s) 35, 275, 636, 872
BsaI GGTCTC 2 cut(s) 110, 465
BsaJI CCNNGG 3 cut(s) 111, 399, 1066
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 561, 933
Bse1I ACTGG 2 cut(s) 588, 1546
Bse3DI GCAATG 2 cut(s) 391, 1416
BseCI ATCGAT 4 cut(s) 35, 275, 636, 872
BseDI CCNNGG 3 cut(s) 111, 399, 1066
BseGI GGATG 4 cut(s) 230, 571, 1468, 1557
BseLI CCNNNNNNNGG 3 cut(s) 221, 561, 933
BseMI GCAATG 2 cut(s) 391, 1416
BseNI ACTGG 2 cut(s) 588, 1546
BsePI GCGCGC 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 1446
BseXI GCAGC 2 cut(s) 1042, 1400
Bsh1236I CGCG 1 cut(s) 487
BshVI ATCGAT 4 cut(s) 35, 275, 636, 872
BsiHKAI GWGCWC 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 1025
BslI CCNNNNNNNGG 3 cut(s) 221, 561, 933
BsmAI GTCTC 6 cut(s) 24, 110, 365, 465, 873, 1112
BsmBI CGTCTC 3 cut(s) 24, 365, 873
BsmI GAATGC 1 cut(s) 943
Bso31I GGTCTC 2 cut(s) 110, 465
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 757, 1446
Bsp1407I TGTACA 2 cut(s) 72, 787
Bsp19I CCATGG 1 cut(s) 1066
BspACI CCGC 5 cut(s) 561, 867, 1071, 1124, 1289
BspDI ATCGAT 4 cut(s) 35, 275, 636, 872
BspFNI CGCG 1 cut(s) 487
BspHI TCATGA 1 cut(s) 1489
BspLI GGNNCC 1 cut(s) 1128
BspOI GCTAGC 1 cut(s) 1355
BspPI GGATC 2 cut(s) 203, 877
BspQI GCTCTTC 1 cut(s) 111
BspTNI GGTCTC 2 cut(s) 110, 465
BsrBI CCGCTC 1 cut(s) 561
BsrDI GCAATG 2 cut(s) 391, 1416
BsrGI TGTACA 2 cut(s) 72, 787
BsrI ACTGG 2 cut(s) 588, 1546
BssECI CCNNGG 3 cut(s) 111, 399, 1066
BssHII GCGCGC 1 cut(s) 485
BssNAI GTATAC 1 cut(s) 1451
BssT1I CCWWGG 1 cut(s) 1066
Bst1107I GTATAC 1 cut(s) 1451
Bst4CI ACNGT 5 cut(s) 787, 826, 993, 1297, 1448
Bst6I CTCTTC 1 cut(s) 111
BstAUI TGTACA 2 cut(s) 72, 787
BstC8I GCNNGC 7 cut(s) 487, 957, 1049, 1053, 1139, 1287, 1353
BstDSI CCRYGG 2 cut(s) 399, 1066
BstF5I GGATG 4 cut(s) 230, 571, 1468, 1557
BstFNI CGCG 1 cut(s) 487
BstHHI GCGC 4 cut(s) 487, 489, 749, 769
BstMAI GTCTC 6 cut(s) 24, 110, 365, 465, 873, 1112
BstMWI GCNNNNNNNGC 1 cut(s) 744
BstSCI CCNGG 1 cut(s) 1023
BstSLI GKGCMC 1 cut(s) 1446
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 1042, 1400
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 1518
BstYI RGATCY 1 cut(s) 1518
BstZ17I GTATAC 1 cut(s) 1451
Bsu15I ATCGAT 4 cut(s) 35, 275, 636, 872
BsuTUI ATCGAT 4 cut(s) 35, 275, 636, 872
BtgI CCRYGG 2 cut(s) 399, 1066
BtgZI GCGATG 2 cut(s) 776, 1468
BtsCI GGATG 4 cut(s) 230, 571, 1468, 1557
BtsI GCAGTG 1 cut(s) 1533
BtsIMutI CAGTG 1 cut(s) 1533
Cac8I GCNNGC 7 cut(s) 487, 957, 1049, 1053, 1139, 1287, 1353
CciI TCATGA 1 cut(s) 1489
CfoI GCGC 4 cut(s) 487, 489, 749, 769
ClaI ATCGAT 4 cut(s) 35, 275, 636, 872
CseI GACGC 4 cut(s) 32, 271, 890, 1199
Csp6I GTAC 2 cut(s) 73, 788
CspCI CAANNNNNGTGG 2 cut(s) 1099, 1134
CviAII CATG 3 cut(s) 1067, 1196, 1490
CviQI GTAC 2 cut(s) 73, 788
DriI GACNNNNNGTC 1 cut(s) 1343
Eam1104I CTCTTC 1 cut(s) 111
Eam1105I GACNNNNNGTC 1 cut(s) 1343
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 1086
Eco130I CCWWGG 1 cut(s) 1066
Eco31I GGTCTC 2 cut(s) 110, 465
Eco88I CYCGRG 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 1066
Esp3I CGTCTC 3 cut(s) 24, 365, 873
FaeI CATG 3 cut(s) 1070, 1199, 1493
FatI CATG 3 cut(s) 1066, 1195, 1489
FauI CCCGC 1 cut(s) 554
FauNDI CATATG 3 cut(s) 324, 434, 453
FbaI TGATCA 3 cut(s) 93, 319, 1576
FblI GTMKAC 1 cut(s) 1450
Fnu4HI GCNGC 2 cut(s) 1056, 1389
FokI GGATG 4 cut(s) 237, 578, 1475, 1564
Fsp4HI GCNGC 2 cut(s) 1056, 1389
FspBI CTAG 2 cut(s) 1181, 1352
GlaI GCGC 4 cut(s) 486, 488, 748, 768
GluI GCNGC 2 cut(s) 1056, 1389
GsuI CTGGAG 1 cut(s) 1563
HapII CCGG 1 cut(s) 1025
HgaI GACGC 4 cut(s) 32, 271, 890, 1199
HhaI GCGC 4 cut(s) 487, 489, 749, 769
Hin1II CATG 3 cut(s) 1070, 1199, 1493
Hin6I GCGC 4 cut(s) 485, 487, 747, 767
HinP1I GCGC 4 cut(s) 485, 487, 747, 767
HincII GTYRAC 3 cut(s) 175, 822, 1567
HindII GTYRAC 3 cut(s) 175, 822, 1567
HindIII AAGCTT 2 cut(s) 477, 1307
HinfI GANTC 7 cut(s) 37, 961, 980, 1010, 1105, 1479, 1555
HpaI GTTAAC 1 cut(s) 822
HpaII CCGG 1 cut(s) 1025
HphI GGTGA 2 cut(s) 38, 668
Hpy166II GTNNAC 4 cut(s) 175, 822, 1451, 1567
Hpy188I TCNGA 8 cut(s) 67, 112, 208, 216, 470, 690, 1210, 1513
Hpy188III TCNNGA 9 cut(s) 91, 292, 364, 1102, 1170, 1490, 1559, 1580, 1602
Hpy8I GTNNAC 4 cut(s) 175, 822, 1451, 1567
Hpy99I CGWCG 2 cut(s) 173, 1231
HpyCH4III ACNGT 5 cut(s) 787, 826, 993, 1297, 1448
HpyCH4IV ACGT 2 cut(s) 171, 1359
HpyCH4V TGCA 7 cut(s) 54, 391, 811, 835, 1321, 1439, 1526
HpyF10VI GCNNNNNNNGC 1 cut(s) 744
HpySE526I ACGT 2 cut(s) 171, 1359
Hsp92II CATG 3 cut(s) 1070, 1199, 1493
HspAI GCGC 4 cut(s) 485, 487, 747, 767
Ksp22I TGATCA 3 cut(s) 93, 319, 1576
KspAI GTTAAC 1 cut(s) 822
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 6 cut(s) 67, 401, 888, 990, 1282, 1544
Lsp1109I GCAGC 2 cut(s) 1042, 1400
LweI GCATC 3 cut(s) 535, 913, 914
MaeI CTAG 2 cut(s) 1181, 1352
MaeII ACGT 2 cut(s) 171, 1359
MaeIII GTNAC 6 cut(s) 44, 523, 616, 724, 962, 1530
MbiI CCGCTC 1 cut(s) 561
MboII GAAGA 5 cut(s) 128, 150, 1090, 1429, 1441
MflI RGATCY 1 cut(s) 1518
MhlI GDGCHC 2 cut(s) 757, 1446
MluCI AATT 5 cut(s) 188, 217, 229, 678, 1434
MlyI GAGTC 3 cut(s) 970, 989, 1114
MmeI TCCRAC 5 cut(s) 45, 654, 1012, 1279, 1536
MnlI CCTC 9 cut(s) 98, 106, 222, 236, 294, 519, 647, 770, 944
MroXI GAANNNNTTC 1 cut(s) 703
MseI TTAA 5 cut(s) 405, 821, 912, 1185, 1254
MslI CAYNNNNRTG 4 cut(s) 456, 762, 1042, 1332
MspA1I CMGCKG 1 cut(s) 1537
MspI CCGG 1 cut(s) 1025
MspR9I CCNGG 1 cut(s) 1025
Mva1269I GAATGC 1 cut(s) 943
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 1 cut(s) 744
NciI CCSGG 1 cut(s) 1025
NcoI CCATGG 1 cut(s) 1066
NdeI CATATG 3 cut(s) 324, 434, 453
NheI GCTAGC 1 cut(s) 1351
NlaIII CATG 3 cut(s) 1070, 1199, 1493
NlaIV GGNNCC 1 cut(s) 1128
NmuCI GTSAC 4 cut(s) 44, 616, 724, 962
OliI CACNNNNGTG 1 cut(s) 762
PagI TCATGA 1 cut(s) 1489
PauI GCGCGC 1 cut(s) 485
PciSI GCTCTTC 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 888
PctI GAATGC 1 cut(s) 943
PdmI GAANNNNTTC 1 cut(s) 703
PfeI GAWTC 4 cut(s) 37, 1010, 1479, 1555
PkrI GCNGC 2 cut(s) 1057, 1390
PleI GAGTC 3 cut(s) 969, 988, 1113
PpsI GAGTC 3 cut(s) 969, 988, 1113
Psp1406I AACGTT 1 cut(s) 1359
PspN4I GGNNCC 1 cut(s) 1128
PsrI GAACNNNNNNTAC 2 cut(s) 449, 481
PsuI RGATCY 1 cut(s) 1518
PteI GCGCGC 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 1537
RsaI GTAC 2 cut(s) 74, 789
RsaNI GTAC 2 cut(s) 73, 788
RseI CAYNNNNRTG 4 cut(s) 456, 762, 1042, 1332
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 5 cut(s) 405, 821, 912, 1185, 1254
SatI GCNGC 2 cut(s) 1056, 1389
SchI GAGTC 3 cut(s) 970, 989, 1114
ScrFI CCNGG 1 cut(s) 1025
SduI GDGCHC 2 cut(s) 757, 1446
SfaNI GCATC 3 cut(s) 535, 913, 914
SmiMI CAYNNNNRTG 4 cut(s) 456, 762, 1042, 1332
SmlI CTYRAG 2 cut(s) 416, 1109
SmoI CTYRAG 2 cut(s) 416, 1109
Sse9I AATT 5 cut(s) 188, 217, 229, 678, 1434
SsiI CCGC 5 cut(s) 561, 867, 1071, 1124, 1289
SspMI CTAG 2 cut(s) 1181, 1352
StyD4I CCNGG 1 cut(s) 1023
StyI CCWWGG 1 cut(s) 1066
TaaI ACNGT 5 cut(s) 787, 826, 993, 1297, 1448
TaiI ACGT 2 cut(s) 174, 1362
TaqII GACCGA 1 cut(s) 1383
TasI AATT 5 cut(s) 188, 217, 229, 678, 1434
TatI WGTACW 2 cut(s) 72, 787
TfiI GAWTC 4 cut(s) 37, 1010, 1479, 1555
Tru1I TTAA 5 cut(s) 405, 821, 912, 1185, 1254
Tru9I TTAA 5 cut(s) 405, 821, 912, 1185, 1254
TscAI CASTG 1 cut(s) 1533
TseFI GTSAC 4 cut(s) 44, 616, 724, 962
TseI GCWGC 2 cut(s) 1055, 1388
Tsp45I GTSAC 4 cut(s) 44, 616, 724, 962
TspGWI ACGGA 4 cut(s) 237, 324, 388, 1287
TspRI CASTG 1 cut(s) 1533
XapI RAATTY 3 cut(s) 188, 229, 678
XmiI GTMKAC 1 cut(s) 1450
XmnI GAANNNNTTC 1 cut(s) 703
XspI CTAG 2 cut(s) 1181, 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.