AT5G08400

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016186
Physical Location & Seq
Reverse (-)
2706458 .. 2708946
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08400.1

Sequence Viewer

Length: 1002 bp
ATGTACGAAAATAATCTCTTAACGCTCTCTTCATGCACCATGAATCTCAATTTCGCTTTCTCCCCATTCCTTGTCTCTCAGAGACAACCATTCTCTTCCCACAAGCGTAATCTTCACACTCTCGTTGCTGTTTCTGCTAATTCCGACAACCTCGCCGGTGAAGATAACGGCGGAATTTCGGCGGCAAATAAAGGTTCTGGGACTACAGCGAGAGGAAGGAGATTACTCAAGGTTAGAGAAGAGAAGCGAAAACGCGACTACGATCGTCTTCACGATTACCCATCTTGGGCCAAGTACTTGTTTCTTTCCTTTTCTTTTGCACTCCAGGTGTTTGTGTTTTTGCCTAAGTCACGAGAAAGTGTGAACCTTTTTTTGGTGAATGATAAATGCAGGGTACTGGAAAGTGCGTGTAAAGATGATGAAGAGCTTCGAGCTGTTCTTGGTGATAGCATAGGAAATCCTGAGCTCATGAGAAAGAAGGTTGAAGAAAGAGTTAGGAAGAAGGGAAAAGATTTCCAGAAACAGAAGACTGGTTCTGTGCTTTCTTTCAAAGTTAACTTCAGAGATTTCAATCCTGTAGATTCCTTCATATGGTTTGAGCTCTATGGAACGCCTTCAGATCGAGATGTTGATCTCATTGGAAGTGTTATACAGGCATGGTATGTTATGGGGCGATTGGGCGCTTTCAATACATCCAACTTGCAGCTAGCAAACACATCCCTAGAATATGATCCTCTCTATGACGCAGAGAAGGGCTTCAAAGTGATGCCTTCATCGTTTCATGACATTAGCGATGTCGAATTTCAAGACAACTGGGGTCGTGTGTGGGTTGATCTTGGTACTTCCGATATCTTCGCCCTTGATGTGCTTCTCAACTGTTTGACAGTTATGAGTTCAGAGTACTTGGGCATTCAACAAGTAGTATTTGGTGGTAAACGAATGGGAGATTGGGAAGAGGGAATGACAAATCCTGATTTTGGGTATAAGTACTTCAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

333

Amino Acids

37.98

Weight (kDa)

6.47

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 184 - 323 2.3e-53 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08400 AT5G08400
fragaria_vesca FvH4_7g04330 FvH4_7g04330 FvH4_7g04330
malus_domestica MD16G1005400.v1.1
prunus_persica Prupe.1G352300_v2.0.a1
pyrus_communis pycom2749g00040
rosa_chinensis RchiOBHm_Chr4g0447251
rosa_laevigata RLG00000005593
rosa_multiflora Rmu_ssc0000396.1_g000003
rosa_roxburghii Rroxscaffold_5G00387610
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4DG448100
rosa_wichuraiana Rw4G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 2 cut(s) 171, 182
AclWI GGATC 1 cut(s) 725
AcsI RAATTY 2 cut(s) 174, 800
AcuI CTGAAG 2 cut(s) 544, 600
AfaI GTAC 6 cut(s) 5, 296, 396, 841, 902, 989
AfiI CCNNNNNNNGG 4 cut(s) 286, 373, 591, 977
AgsI TTSAA 8 cut(s) 485, 550, 571, 688, 760, 806, 914, 994
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 4 cut(s) 356, 388, 931, 963
AluBI AGCT 5 cut(s) 427, 434, 466, 601, 706
AluI AGCT 5 cut(s) 427, 434, 466, 601, 706
Alw21I GWGCWC 2 cut(s) 468, 603
Alw26I GTCTC 2 cut(s) 76, 79
AlwI GGATC 1 cut(s) 725
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 1 cut(s) 703
ApoI RAATTY 2 cut(s) 174, 800
Asp700I GAANNNNTTC 3 cut(s) 426, 613, 755
AspLEI GCGC 1 cut(s) 683
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 3 cut(s) 170, 388, 455
AsuNHI GCTAGC 1 cut(s) 706
BanII GRGCYC 2 cut(s) 468, 603
BarI GAAGNNNNNNTAC 1 cut(s) 974
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 2 cut(s) 260, 533
Bbv12I GWGCWC 2 cut(s) 468, 603
BbvI GCAGC 1 cut(s) 715
BccI CCATC 1 cut(s) 289
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 76, 79
BfaI CTAG 2 cut(s) 707, 722
BfmI CTRYAG 2 cut(s) 204, 576
BfoI RGCGCY 1 cut(s) 684
BglII AGATCT 1 cut(s) 996
BisI GCNGC 2 cut(s) 183, 704
BlsI GCNGC 2 cut(s) 184, 705
BmcAI AGTACT 3 cut(s) 296, 902, 989
Bme1390I CCNGG 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 823
BmsI GCATC 1 cut(s) 756
BmtI GCTAGC 1 cut(s) 710
BmuI ACTGGG 1 cut(s) 823
BpiI GAAGAC 2 cut(s) 260, 533
BpmI CTGGAG 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 212
BsaBI GATNNNNATC 2 cut(s) 570, 630
Bsc4I CCNNNNNNNGG 4 cut(s) 286, 373, 591, 977
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 3 cut(s) 402, 535, 818
Bse8I GATNNNNATC 2 cut(s) 570, 630
BseBI CCWGG 1 cut(s) 326
BseGI GGATG 2 cut(s) 692, 716
BseJI GATNNNNATC 2 cut(s) 570, 630
BseLI CCNNNNNNNGG 4 cut(s) 286, 373, 591, 977
BseMII CTCAG 2 cut(s) 92, 453
BseNI ACTGG 3 cut(s) 402, 535, 818
BseXI GCAGC 1 cut(s) 715
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 468, 603
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 4 cut(s) 286, 373, 591, 977
BsmAI GTCTC 2 cut(s) 76, 79
BsmFI GGGAC 1 cut(s) 214
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 468, 603
Bsp143I GATC 6 cut(s) 262, 619, 631, 730, 832, 996
BspACI CCGC 2 cut(s) 171, 182
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 2 cut(s) 91, 454
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 2 cut(s) 468, 781
BspOI GCTAGC 1 cut(s) 710
BspPI GGATC 1 cut(s) 725
BspQI GCTCTTC 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 3 cut(s) 402, 535, 818
BssAI RCCGGY 1 cut(s) 155
BssMI GATC 6 cut(s) 262, 619, 631, 730, 832, 996
BssSI CACGAG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 878, 886
Bst6I CTCTTC 5 cut(s) 34, 100, 234, 417, 948
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 3 cut(s) 78, 345, 462
BstF5I GGATG 2 cut(s) 692, 716
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 1 cut(s) 683
BstKTI GATC 6 cut(s) 265, 622, 634, 733, 835, 999
BstMAI GTCTC 2 cut(s) 76, 79
BstMBI GATC 6 cut(s) 262, 619, 631, 730, 832, 996
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 2 cut(s) 204, 576
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 715
BstV2I GAAGAC 2 cut(s) 260, 533
BstX2I RGATCY 1 cut(s) 996
BstYI RGATCY 1 cut(s) 996
BsuRI GGCC 1 cut(s) 290
BtgZI GCGATG 1 cut(s) 807
BtsCI GGATG 2 cut(s) 692, 716
Cac8I GCNNGC 1 cut(s) 708
CciI TCATGA 2 cut(s) 468, 781
CfoI GCGC 1 cut(s) 683
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 288
CseI GACGC 1 cut(s) 752
Csp6I GTAC 6 cut(s) 4, 295, 395, 840, 901, 988
CviAII CATG 5 cut(s) 33, 40, 469, 657, 782
CviJI RGCY 7 cut(s) 290, 427, 434, 466, 601, 706, 756
CviKI_1 RGCY 7 cut(s) 290, 427, 434, 466, 601, 706, 756
CviQI GTAC 6 cut(s) 4, 295, 395, 840, 901, 988
DdeI CTNAG 3 cut(s) 78, 345, 462
DpnI GATC 6 cut(s) 264, 621, 633, 732, 834, 998
DpnII GATC 6 cut(s) 262, 619, 631, 730, 832, 996
Eam1104I CTCTTC 5 cut(s) 34, 100, 234, 417, 948
EarI CTCTTC 5 cut(s) 34, 100, 234, 417, 948
EciI GGCGGA 1 cut(s) 186
Ecl136II GAGCTC 2 cut(s) 466, 601
Eco24I GRGCYC 2 cut(s) 468, 603
Eco32I GATATC 1 cut(s) 850
Eco53kI GAGCTC 2 cut(s) 466, 601
Eco57I CTGAAG 2 cut(s) 544, 600
EcoICRI GAGCTC 2 cut(s) 466, 601
EcoRII CCWGG 1 cut(s) 324
EcoRV GATATC 1 cut(s) 850
EcoT38I GRGCYC 2 cut(s) 468, 603
FaeI CATG 5 cut(s) 36, 43, 472, 660, 785
FaqI GGGAC 1 cut(s) 214
FatI CATG 5 cut(s) 32, 39, 468, 656, 781
FauNDI CATATG 1 cut(s) 590
Fnu4HI GCNGC 2 cut(s) 183, 704
FokI GGATG 2 cut(s) 679, 703
FriOI GRGCYC 2 cut(s) 468, 603
Fsp4HI GCNGC 2 cut(s) 183, 704
FspBI CTAG 2 cut(s) 707, 722
GlaI GCGC 1 cut(s) 682
GluI GCNGC 2 cut(s) 183, 704
GsuI CTGGAG 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 1 cut(s) 290
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 752
HhaI GCGC 1 cut(s) 683
Hin1II CATG 5 cut(s) 36, 43, 472, 660, 785
Hin6I GCGC 1 cut(s) 681
HinP1I GCGC 1 cut(s) 681
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 2 cut(s) 43, 581
HpaI GTTAAC 1 cut(s) 556
HpaII CCGG 1 cut(s) 156
HphI GGTGA 3 cut(s) 170, 388, 455
Hpy166II GTNNAC 3 cut(s) 364, 556, 935
Hpy188I TCNGA 7 cut(s) 81, 145, 563, 619, 847, 898, 1001
Hpy8I GTNNAC 3 cut(s) 364, 556, 935
HpyAV CCTTC 7 cut(s) 210, 472, 496, 595, 624, 745, 780
HpyCH4III ACNGT 2 cut(s) 878, 886
HpyCH4V TGCA 4 cut(s) 36, 320, 390, 703
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 78, 345, 462
Hsp92II CATG 5 cut(s) 36, 43, 472, 660, 785
HspAI GCGC 1 cut(s) 681
KspAI GTTAAC 1 cut(s) 556
Kzo9I GATC 6 cut(s) 262, 619, 631, 730, 832, 996
LguI GCTCTTC 1 cut(s) 417
Lsp1109I GCAGC 1 cut(s) 715
LweI GCATC 1 cut(s) 756
MaeI CTAG 2 cut(s) 707, 722
MaeIII GTNAC 1 cut(s) 348
MalI GATC 6 cut(s) 264, 621, 633, 732, 834, 998
MboI GATC 6 cut(s) 262, 619, 631, 730, 832, 996
MflI RGATCY 1 cut(s) 996
MhlI GDGCHC 2 cut(s) 468, 603
MluCI AATT 4 cut(s) 49, 139, 174, 800
MmeI TCCRAC 2 cut(s) 168, 720
MnlI CCTC 4 cut(s) 161, 206, 744, 949
MroXI GAANNNNTTC 3 cut(s) 426, 613, 755
MseI TTAA 2 cut(s) 20, 555
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 909
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeI CATATG 1 cut(s) 590
NdeII GATC 6 cut(s) 262, 619, 631, 730, 832, 996
NheI GCTAGC 1 cut(s) 706
NlaIII CATG 5 cut(s) 36, 43, 472, 660, 785
NmuCI GTSAC 1 cut(s) 348
PagI TCATGA 2 cut(s) 468, 781
PciSI GCTCTTC 1 cut(s) 417
PctI GAATGC 1 cut(s) 909
PdmI GAANNNNTTC 3 cut(s) 426, 613, 755
PfeI GAWTC 2 cut(s) 43, 581
PkrI GCNGC 2 cut(s) 184, 705
Ple19I CGATCG 1 cut(s) 265
Psp124BI GAGCTC 2 cut(s) 468, 603
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspPI GGNCC 1 cut(s) 288
PsuI RGATCY 1 cut(s) 996
PvuI CGATCG 1 cut(s) 265
RsaI GTAC 6 cut(s) 5, 296, 396, 841, 902, 989
RsaNI GTAC 6 cut(s) 4, 295, 395, 840, 901, 988
SacI GAGCTC 2 cut(s) 468, 603
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 2 cut(s) 20, 555
SatI GCNGC 2 cut(s) 183, 704
Sau3AI GATC 6 cut(s) 262, 619, 631, 730, 832, 996
Sau96I GGNCC 1 cut(s) 288
ScaI AGTACT 3 cut(s) 296, 902, 989
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 468, 603
SfaNI GCATC 1 cut(s) 756
SfcI CTRYAG 2 cut(s) 204, 576
SgrAI CRCCGGYG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 4 cut(s) 49, 139, 174, 800
SsiI CCGC 2 cut(s) 171, 182
SspMI CTAG 2 cut(s) 707, 722
SstI GAGCTC 2 cut(s) 468, 603
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 878, 886
TaqI TCGA 3 cut(s) 430, 622, 798
TasI AATT 4 cut(s) 49, 139, 174, 800
TatI WGTACW 3 cut(s) 294, 900, 987
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 2 cut(s) 43, 581
Tru1I TTAA 2 cut(s) 20, 555
Tru9I TTAA 2 cut(s) 20, 555
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 1 cut(s) 703
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 6 cut(s) 21, 56, 435, 577, 762, 770
XapI RAATTY 2 cut(s) 174, 800
XmnI GAANNNNTTC 3 cut(s) 426, 613, 755
XspI CTAG 2 cut(s) 707, 722
ZrmI AGTACT 3 cut(s) 296, 902, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.