Rroxscaffold_5G00387610

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016186
Physical Location & Seq
Forward (+)
66192317 .. 66194998
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387610.1

Sequence Viewer

Length: 867 bp
ATGAAGGCGGCCATGGCGATGGTTAGAGGCGCGGCCAACGGCGGGTTTAGAATTGATTCGGAGGAACATTCCTTTTCCTTGTTTCATCAGGTAACGGTACAGCCAACTCGCCGTCACCGACTAATAACAGTATGTGCAAGCTCGGATGGGGCAAAAGGCTCAGGAACCACAGCCAGAGGACGGAGACTTATCAAAATTCGTGAAGATAAGAGGAAGCGCGAGTATGAGCGCCTTCACAACTATCCTGCTTGGGCCAAAGTGTTAGAAAACGCCTGCAAAGACGATGCTGAGCTTAGAGCCCTTCTGGGAGATAGCATTGGAAACCCTGAGCTCATGAGGAAAAGGGTTGAACAGAGAGTTCGCCAGAAGGGTAGAGACTTCCGCAAGTCCAAAACCGGTTCACTCCTTGCCTTCAAAGTCTCCTTCAGGGACTTCAACCCCCTTGATTCCTACATTTGGTTTGAATTCTTTGGATCACCGTCTGATCGTGATATTGATCTCTTTGGTAGTGTTATTCAGTCATGGTATGTTATGGGACGCTTGGGTGCCTTTAATTCTACTAATCTGCAGTTGGCAAATTCATCTATGGAGTACAATCCCCTCTATGATGTGGATAAGGGTTTCAAAGTGATGCCATCTTCATTTCATGAGATCAGTGACATTGAGTTCCAGGATAACTGGGGTCGTGTTTGGGTAGACCTGGGTACAGCTGACTATTTTGCTGTTGATGTACTGCTCAACTGCTTGACAGTCTTAAGTTCAGAATATATAGGCGTTCAACAGATAGTTTTTGGTGGTCAGAGAATCGGTGATTGGGAAGAGGGGATGACCAACGCAGAGTATGGATATAAGTACTTCAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

288

Amino Acids

32.94

Weight (kDa)

8.64

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 139 - 278 1.5e-52 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08400 AT5G08400
fragaria_vesca FvH4_7g04330 FvH4_7g04330 FvH4_7g04330
malus_domestica MD16G1005400.v1.1
prunus_persica Prupe.1G352300_v2.0.a1
pyrus_communis pycom2749g00040
rosa_chinensis RchiOBHm_Chr4g0447251
rosa_laevigata RLG00000005593
rosa_multiflora Rmu_ssc0000396.1_g000003
rosa_roxburghii Rroxscaffold_5G00387610
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4DG448100
rosa_wichuraiana Rw4G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 696
AccII CGCG 2 cut(s) 32, 219
AciI CCGC 4 cut(s) 8, 32, 42, 382
AclWI GGATC 1 cut(s) 481
AcoI YGGCCR 2 cut(s) 9, 33
AcsI RAATTY 3 cut(s) 195, 464, 577
AcuI CTGAAG 1 cut(s) 409
AfaI GTAC 5 cut(s) 99, 593, 706, 732, 854
AfiI CCNNNNNNNGG 3 cut(s) 42, 180, 456
AflII CTTAAG 1 cut(s) 754
AgeI ACCGGT 1 cut(s) 395
AgsI TTSAA 7 cut(s) 350, 415, 436, 464, 625, 779, 859
AjnI CCWGG 2 cut(s) 669, 699
AjuI GAANNNNNNNTTGG 2 cut(s) 796, 828
AluBI AGCT 4 cut(s) 141, 292, 331, 710
AluI AGCT 4 cut(s) 141, 292, 331, 710
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 3 cut(s) 178, 369, 424
AlwI GGATC 1 cut(s) 481
AoxI GGCC 3 cut(s) 9, 33, 252
ApoI RAATTY 3 cut(s) 195, 464, 577
AsiGI ACCGGT 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 55
AspLEI GCGC 3 cut(s) 32, 219, 231
AspS9I GGNCC 1 cut(s) 252
AsuHPI GGTGA 3 cut(s) 107, 468, 821
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 2 cut(s) 301, 333
Bbv12I GWGCWC 1 cut(s) 333
BccI CCATC 3 cut(s) 13, 140, 643
BceAI ACGGC 2 cut(s) 55, 96
BciT130I CCWGG 2 cut(s) 671, 701
BcoDI GTCTC 3 cut(s) 178, 369, 424
BfmI CTRYAG 1 cut(s) 566
BfoI RGCGCY 1 cut(s) 232
BfrI CTTAAG 1 cut(s) 754
BisI GCNGC 2 cut(s) 9, 33
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 2 cut(s) 10, 34
BmcAI AGTACT 1 cut(s) 854
Bme1390I CCNGG 2 cut(s) 671, 701
BmgT120I GGNCC 1 cut(s) 252
BmiI GGNNCC 2 cut(s) 166, 547
BmrFI CCNGG 2 cut(s) 671, 701
BmrI ACTGGG 1 cut(s) 688
BmsI GCATC 2 cut(s) 274, 621
BmuI ACTGGG 1 cut(s) 688
Bpu10I CCTNAGC 2 cut(s) 160, 327
Bpu1102I GCTNAGC 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 12, 700
BsaWI WCCGGW 1 cut(s) 395
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 180, 456
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 683
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 2 cut(s) 671, 701
BseDI CCNNGG 2 cut(s) 12, 700
BseGI GGATG 2 cut(s) 151, 831
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 3 cut(s) 42, 180, 456
BseMII CTCAG 3 cut(s) 174, 279, 318
BseNI ACTGG 1 cut(s) 683
Bsh1236I CGCG 2 cut(s) 32, 219
BshFI GGCC 3 cut(s) 11, 35, 254
BshNI GGYRCC 1 cut(s) 545
BshTI ACCGGT 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 1 cut(s) 396
BslFI GGGAC 2 cut(s) 443, 549
BslI CCNNNNNNNGG 3 cut(s) 42, 180, 456
BsmAI GTCTC 3 cut(s) 178, 369, 424
BsmFI GGGAC 2 cut(s) 443, 549
BsnI GGCC 3 cut(s) 11, 35, 254
Bsp1286I GDGCHC 2 cut(s) 301, 333
Bsp143I GATC 4 cut(s) 473, 484, 496, 651
Bsp1720I GCTNAGC 1 cut(s) 288
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 4 cut(s) 8, 32, 42, 382
BspANI GGCC 3 cut(s) 11, 35, 254
BspCNI CTCAG 3 cut(s) 173, 280, 319
BspFNI CGCG 2 cut(s) 32, 219
BspHI TCATGA 2 cut(s) 333, 646
BspLI GGNNCC 2 cut(s) 166, 547
BspMAI CTGCAG 1 cut(s) 570
BspPI GGATC 1 cut(s) 481
BspT107I GGYRCC 1 cut(s) 545
BspTI CTTAAG 1 cut(s) 754
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 1 cut(s) 683
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 12, 700
BssMI GATC 4 cut(s) 473, 484, 496, 651
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 2 cut(s) 671, 701
Bst4CI ACNGT 4 cut(s) 97, 130, 480, 751
Bst6I CTCTTC 1 cut(s) 813
BstAFI CTTAAG 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 139, 274
BstDEI CTNAG 4 cut(s) 160, 288, 293, 327
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 151, 831
BstFNI CGCG 2 cut(s) 32, 219
BstH2I RGCGCY 1 cut(s) 232
BstHHI GCGC 3 cut(s) 32, 219, 231
BstKTI GATC 4 cut(s) 476, 487, 499, 654
BstMAI GTCTC 3 cut(s) 178, 369, 424
BstMBI GATC 4 cut(s) 473, 484, 496, 651
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 2 cut(s) 671, 701
BstSCI CCNGG 2 cut(s) 669, 699
BstSFI CTRYAG 1 cut(s) 566
BstUI CGCG 2 cut(s) 32, 219
BstXI CCANNNNNNTGG 1 cut(s) 19
BsuRI GGCC 3 cut(s) 11, 35, 254
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 2 cut(s) 151, 831
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 139, 274
CciI TCATGA 2 cut(s) 333, 646
CfoI GCGC 3 cut(s) 32, 219, 231
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 252
CseI GACGC 1 cut(s) 546
Csp6I GTAC 5 cut(s) 98, 592, 705, 731, 853
CspAI ACCGGT 1 cut(s) 395
CviAII CATG 4 cut(s) 13, 334, 522, 647
CviQI GTAC 5 cut(s) 98, 592, 705, 731, 853
DdeI CTNAG 4 cut(s) 160, 288, 293, 327
DpnI GATC 4 cut(s) 475, 486, 498, 653
DpnII GATC 4 cut(s) 473, 484, 496, 651
EaeI YGGCCR 2 cut(s) 9, 33
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 301, 333
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 669, 699
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 2 cut(s) 301, 333
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 4 cut(s) 16, 337, 525, 650
FaqI GGGAC 2 cut(s) 443, 549
FatI CATG 4 cut(s) 12, 333, 521, 646
FauI CCCGC 1 cut(s) 35
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 2 cut(s) 9, 33
FokI GGATG 2 cut(s) 158, 838
FriOI GRGCYC 2 cut(s) 301, 333
Fsp4HI GCNGC 2 cut(s) 9, 33
GlaI GCGC 3 cut(s) 31, 218, 230
GluI GCNGC 2 cut(s) 9, 33
HaeII RGCGCY 1 cut(s) 232
HaeIII GGCC 3 cut(s) 11, 35, 254
HapII CCGG 1 cut(s) 396
HgaI GACGC 1 cut(s) 546
HhaI GCGC 3 cut(s) 32, 219, 231
Hin1II CATG 4 cut(s) 16, 337, 525, 650
Hin6I GCGC 3 cut(s) 30, 217, 229
HinP1I GCGC 3 cut(s) 30, 217, 229
HinfI GANTC 3 cut(s) 56, 446, 804
HpaII CCGG 1 cut(s) 396
HphI GGTGA 3 cut(s) 107, 468, 821
Hpy166II GTNNAC 2 cut(s) 401, 697
Hpy188I TCNGA 5 cut(s) 61, 145, 484, 763, 801
Hpy188III TCNNGA 6 cut(s) 162, 200, 334, 488, 647, 859
Hpy8I GTNNAC 2 cut(s) 401, 697
HpyAV CCTTC 5 cut(s) 242, 311, 361, 421, 433
HpyCH4III ACNGT 4 cut(s) 97, 130, 480, 751
HpyCH4V TGCA 3 cut(s) 137, 276, 568
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 4 cut(s) 160, 288, 293, 327
Hsp92II CATG 4 cut(s) 16, 337, 525, 650
HspAI GCGC 3 cut(s) 30, 217, 229
Kzo9I GATC 4 cut(s) 473, 484, 496, 651
LweI GCATC 2 cut(s) 274, 621
MaeIII GTNAC 3 cut(s) 91, 113, 656
MalI GATC 4 cut(s) 475, 486, 498, 653
MboI GATC 4 cut(s) 473, 484, 496, 651
MboII GAAGA 3 cut(s) 215, 630, 830
MhlI GDGCHC 2 cut(s) 301, 333
MluCI AATT 5 cut(s) 51, 195, 464, 553, 577
MnlI CCTC 7 cut(s) 20, 55, 170, 204, 330, 611, 814
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 2 cut(s) 552, 755
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 710
MspCI CTTAAG 1 cut(s) 754
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 2 cut(s) 671, 701
MvaI CCWGG 2 cut(s) 671, 701
MvnI CGCG 2 cut(s) 32, 219
MwoI GCNNNNNNNGC 1 cut(s) 14
NcoI CCATGG 1 cut(s) 12
NdeII GATC 4 cut(s) 473, 484, 496, 651
NlaIII CATG 4 cut(s) 16, 337, 525, 650
NlaIV GGNNCC 2 cut(s) 166, 547
NmuCI GTSAC 2 cut(s) 113, 656
PagI TCATGA 2 cut(s) 333, 646
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 56, 446, 804
PfoI TCCNGGA 1 cut(s) 669
PinAI ACCGGT 1 cut(s) 395
PkrI GCNGC 2 cut(s) 10, 34
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 669, 699
PspGI CCWGG 2 cut(s) 669, 699
PspN4I GGNNCC 2 cut(s) 166, 547
PspPI GGNCC 1 cut(s) 252
PstI CTGCAG 1 cut(s) 570
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 5 cut(s) 99, 593, 706, 732, 854
RsaNI GTAC 5 cut(s) 98, 592, 705, 731, 853
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 552, 755
SatI GCNGC 2 cut(s) 9, 33
Sau3AI GATC 4 cut(s) 473, 484, 496, 651
Sau96I GGNCC 1 cut(s) 252
ScaI AGTACT 1 cut(s) 854
ScrFI CCNGG 2 cut(s) 671, 701
SduI GDGCHC 2 cut(s) 301, 333
SetI ASST 6 cut(s) 93, 143, 294, 333, 702, 712
SfaNI GCATC 2 cut(s) 274, 621
SfcI CTRYAG 1 cut(s) 566
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 754
SmoI CTYRAG 1 cut(s) 754
Sse9I AATT 5 cut(s) 51, 195, 464, 553, 577
SsiI CCGC 4 cut(s) 8, 32, 42, 382
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 669, 699
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 4 cut(s) 97, 130, 480, 751
TasI AATT 5 cut(s) 51, 195, 464, 553, 577
TatI WGTACW 3 cut(s) 591, 730, 852
TauI GCSGC 2 cut(s) 11, 35
TfiI GAWTC 3 cut(s) 56, 446, 804
Tru1I TTAA 2 cut(s) 552, 755
Tru9I TTAA 2 cut(s) 552, 755
TscAI CASTG 1 cut(s) 661
TseFI GTSAC 2 cut(s) 113, 656
Tsp45I GTSAC 2 cut(s) 113, 656
TspDTI ATGAA 5 cut(s) 17, 74, 570, 630, 635
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 1 cut(s) 661
Vha464I CTTAAG 1 cut(s) 754
XapI RAATTY 3 cut(s) 195, 464, 577
XmiI GTMKAC 1 cut(s) 696
XmnI GAANNNNTTC 1 cut(s) 55
ZrmI AGTACT 1 cut(s) 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.