MD16G1005400.v1.1

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016186
Physical Location & Seq
Forward (+)
403275 .. 405557
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1005400.v1.1.491

Sequence Viewer

Length: 897 bp
ATGATGGCGGCCTTGGTCACAGGAGGAGCCAAACTGGAGGGTTCAGGCTTTATCTCGCTACAGAATATTAATTACAACAATACACGAGGAAGAAGAAGAAGAAACGAAGAAACCAACTGTCGACCTCATCCACCTCCACCACCTCGGCTCCTATCACTAGTAGTATGCCAGGCCGAGGCGGCCAAAGGGTCAGGTACCACCGCCAGAGGACGCAGACTTGTTAAAATTCGTGAAGAGAAGAGGAAGCGCGAGTACGACCGCCTTCACAGGTATCCAGCCTGGGCCAAAGTGTTGGAAGACGCCTGCAAGGACGATGGTGAGCTGCGAGCGGTTCTTGGAGACAGCATTGGGAACCCTGAGCTCATGAGGAAAAGGGTCGAAGAAAGAATCCGCCAGAAGGGCAGGGACTTCACCAAATCCAAAACCGGTTCTGTCCTTGCCTTCAAAGTCACTTTCAGAGACTTCAATCCTCTTGATTCCCACATATGGTTTGAATTCTATGGATCCCCATCTGATCGCGATGTTGATCTCTTCGGCAGTGTTATTCAATCATGGTATGTCATGGGACGCTTGGGTGCCTTTAATTCTACTAATCTGCAGCTGGCAAATTCTTCTATGGAGTACAATCCCCTTTATGATGTGGATAAGGGTTTCAAAGTGATGCCATCTTCATTTCATGATATCAGCGACGTTGAGTTTCAGGATAACTGGGGTCGTGTTTGGGTAGACCTTGGTACAGCTGACTATTTTTCTGTGGATGTACTCCTCAATTGCTTGACAGTCTTGAGTACAGAATATCTGGGCATTCAACAGATAGTTTTTGGTGGCCGTAACATGGGTGATTGGGAAGAGGGGATGACCAACACAGAGTATGGATACAAGTACTTCAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

299

Amino Acids

34.06

Weight (kDa)

8.56

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 149 - 288 1.1e-52 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08400 AT5G08400
fragaria_vesca FvH4_7g04330 FvH4_7g04330 FvH4_7g04330
malus_domestica MD16G1005400.v1.1
prunus_persica Prupe.1G352300_v2.0.a1
pyrus_communis pycom2749g00040
rosa_chinensis RchiOBHm_Chr4g0447251
rosa_laevigata RLG00000005593
rosa_multiflora Rmu_ssc0000396.1_g000003
rosa_roxburghii Rroxscaffold_5G00387610
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4DG448100
rosa_wichuraiana Rw4G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 194, 575
AccBSI CCGCTC 1 cut(s) 329
AccI GTMKAC 2 cut(s) 121, 726
AccII CGCG 2 cut(s) 249, 519
AciI CCGC 6 cut(s) 8, 179, 201, 259, 329, 391
AclWI GGATC 2 cut(s) 498, 511
AcoI YGGCCR 2 cut(s) 180, 826
AcsI RAATTY 3 cut(s) 225, 494, 607
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 7 cut(s) 196, 254, 623, 736, 762, 790, 884
AfiI CCNNNNNNNGG 4 cut(s) 175, 397, 486, 835
AgeI ACCGGT 1 cut(s) 425
AgsI TTSAA 7 cut(s) 445, 466, 494, 548, 655, 809, 889
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 2 cut(s) 168, 278
AluBI AGCT 4 cut(s) 322, 361, 601, 740
AluI AGCT 4 cut(s) 322, 361, 601, 740
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 333, 453
AlwI GGATC 2 cut(s) 498, 511
AoxI GGCC 5 cut(s) 9, 171, 180, 282, 826
ApeKI GCWGC 2 cut(s) 322, 598
ApoI RAATTY 3 cut(s) 225, 494, 607
AseI ATTAAT 1 cut(s) 69
AsiGI ACCGGT 1 cut(s) 425
Asp718I GGTACC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 3 cut(s) 329, 403, 851
BamHI GGATCC 1 cut(s) 503
BanI GGYRCC 2 cut(s) 194, 575
BanII GRGCYC 1 cut(s) 363
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
BauI CACGAG 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 309, 610
BccI CCATC 3 cut(s) 308, 517, 673
BceAI ACGGC 1 cut(s) 813
BciT130I CCWGG 2 cut(s) 170, 280
BciVI GTATCC 2 cut(s) 282, 869
BcoDI GTCTC 2 cut(s) 333, 453
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 2 cut(s) 59, 596
BfuI GTATCC 2 cut(s) 282, 869
BglI GCCNNNNNGGC 2 cut(s) 179, 399
BglII AGATCT 1 cut(s) 891
BisI GCNGC 4 cut(s) 9, 180, 323, 599
BlsI GCNGC 4 cut(s) 10, 181, 324, 600
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 2 cut(s) 170, 280
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 6 cut(s) 28, 149, 196, 353, 505, 577
BmrFI CCNGG 2 cut(s) 170, 280
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 1 cut(s) 651
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 805
BsaBI GATNNNNATC 2 cut(s) 508, 525
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 5 cut(s) 12, 143, 174, 279, 730
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 397, 486, 835
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 2 cut(s) 39, 713
Bse8I GATNNNNATC 2 cut(s) 508, 525
BseBI CCWGG 2 cut(s) 170, 280
BseDI CCNNGG 5 cut(s) 12, 143, 174, 279, 730
BseGI GGATG 3 cut(s) 127, 763, 861
BseJI GATNNNNATC 2 cut(s) 508, 525
BseLI CCNNNNNNNGG 4 cut(s) 175, 397, 486, 835
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 39, 713
BseRI GAGGAG 2 cut(s) 39, 755
BseXI GCAGC 2 cut(s) 309, 610
Bsh1236I CGCG 2 cut(s) 249, 519
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 5 cut(s) 11, 173, 182, 284, 828
BshNI GGYRCC 2 cut(s) 194, 575
BshTI ACCGGT 1 cut(s) 425
BsiEI CGRYCG 1 cut(s) 259
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 2 cut(s) 419, 579
BslI CCNNNNNNNGG 4 cut(s) 175, 397, 486, 835
BsmAI GTCTC 2 cut(s) 333, 453
BsmFI GGGAC 2 cut(s) 419, 579
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 5 cut(s) 11, 173, 182, 284, 828
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 4 cut(s) 503, 514, 526, 891
Bsp68I TCGCGA 1 cut(s) 519
BspACI CCGC 6 cut(s) 8, 179, 201, 259, 329, 391
BspANI GGCC 5 cut(s) 11, 173, 182, 284, 828
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 2 cut(s) 249, 519
BspHI TCATGA 2 cut(s) 363, 676
BspLI GGNNCC 6 cut(s) 28, 149, 196, 353, 505, 577
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 2 cut(s) 498, 511
BspT107I GGYRCC 2 cut(s) 194, 575
BsrBI CCGCTC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 2 cut(s) 39, 713
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 5 cut(s) 12, 143, 174, 279, 730
BssMI GATC 4 cut(s) 503, 514, 526, 891
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 12, 730
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 170, 280
Bst4CI ACNGT 2 cut(s) 119, 781
Bst6I CTCTTC 4 cut(s) 228, 233, 536, 843
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 304, 327, 603
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 127, 763, 861
BstFNI CGCG 2 cut(s) 249, 519
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 4 cut(s) 506, 517, 529, 894
BstMAI GTCTC 2 cut(s) 333, 453
BstMBI GATC 4 cut(s) 503, 514, 526, 891
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 2 cut(s) 179, 399
BstNI CCWGG 2 cut(s) 170, 280
BstSCI CCNGG 2 cut(s) 168, 278
BstSFI CTRYAG 2 cut(s) 59, 596
BstUI CGCG 2 cut(s) 249, 519
BstV1I GCAGC 2 cut(s) 309, 610
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 2 cut(s) 503, 891
BstXI CCANNNNNNTGG 1 cut(s) 292
BstYI RGATCY 2 cut(s) 503, 891
BsuI GTATCC 2 cut(s) 282, 869
BsuRI GGCC 5 cut(s) 11, 173, 182, 284, 828
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 3 cut(s) 127, 763, 861
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 544
BtuMI TCGCGA 1 cut(s) 519
Cac8I GCNNGC 3 cut(s) 304, 327, 603
CciI TCATGA 2 cut(s) 363, 676
CfoI GCGC 1 cut(s) 249
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 3 cut(s) 219, 308, 576
Csp6I GTAC 7 cut(s) 195, 253, 622, 735, 761, 789, 883
CspAI ACCGGT 1 cut(s) 425
CviAII CATG 5 cut(s) 364, 552, 562, 677, 835
CviQI GTAC 7 cut(s) 195, 253, 622, 735, 761, 789, 883
DdeI CTNAG 1 cut(s) 357
DpnI GATC 4 cut(s) 505, 516, 528, 893
DpnII GATC 4 cut(s) 503, 514, 526, 891
EaeI YGGCCR 2 cut(s) 180, 826
Eam1104I CTCTTC 4 cut(s) 228, 233, 536, 843
EarI CTCTTC 4 cut(s) 228, 233, 536, 843
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 2 cut(s) 12, 730
Eco24I GRGCYC 1 cut(s) 363
Eco32I GATATC 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 494
EcoRII CCWGG 2 cut(s) 168, 278
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 2 cut(s) 12, 730
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 2 cut(s) 12, 730
FaeI CATG 5 cut(s) 367, 555, 565, 680, 838
FaqI GGGAC 2 cut(s) 419, 579
FatI CATG 5 cut(s) 363, 551, 561, 676, 834
FauNDI CATATG 1 cut(s) 485
FblI GTMKAC 2 cut(s) 121, 726
Fnu4HI GCNGC 4 cut(s) 9, 180, 323, 599
FokI GGATG 3 cut(s) 114, 770, 868
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 4 cut(s) 9, 180, 323, 599
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 248
GluI GCNGC 4 cut(s) 9, 180, 323, 599
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 5 cut(s) 11, 173, 182, 284, 828
HapII CCGG 1 cut(s) 426
HgaI GACGC 3 cut(s) 219, 308, 576
HhaI GCGC 1 cut(s) 249
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 5 cut(s) 367, 555, 565, 680, 838
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 122
HindII GTYRAC 1 cut(s) 122
HinfI GANTC 2 cut(s) 387, 476
HpaII CCGG 1 cut(s) 426
HphI GGTGA 3 cut(s) 329, 403, 851
Hpy166II GTNNAC 2 cut(s) 122, 727
Hpy188I TCNGA 3 cut(s) 458, 514, 896
Hpy188III TCNNGA 8 cut(s) 230, 364, 473, 518, 677, 701, 784, 889
Hpy8I GTNNAC 2 cut(s) 122, 727
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 3 cut(s) 272, 391, 451
HpyCH4III ACNGT 2 cut(s) 119, 781
HpyCH4IV ACGT 1 cut(s) 690
HpyCH4V TGCA 2 cut(s) 306, 598
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 399
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 690
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 5 cut(s) 367, 555, 565, 680, 838
HspAI GCGC 1 cut(s) 247
KpnI GGTACC 1 cut(s) 198
Kzo9I GATC 4 cut(s) 503, 514, 526, 891
LmnI GCTCC 2 cut(s) 26, 153
Lsp1109I GCAGC 2 cut(s) 309, 610
LweI GCATC 1 cut(s) 651
MaeI CTAG 1 cut(s) 158
MaeII ACGT 1 cut(s) 690
MaeIII GTNAC 3 cut(s) 16, 448, 830
MalI GATC 4 cut(s) 505, 516, 528, 893
MbiI CCGCTC 1 cut(s) 329
MboI GATC 4 cut(s) 503, 514, 526, 891
MfeI CAATTG 1 cut(s) 769
MflI RGATCY 2 cut(s) 503, 891
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 6 cut(s) 70, 225, 494, 583, 607, 769
MmeI TCCRAC 1 cut(s) 273
MseI TTAA 3 cut(s) 69, 222, 582
MspA1I CMGCKG 2 cut(s) 601, 740
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 2 cut(s) 170, 280
MunI CAATTG 1 cut(s) 769
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 2 cut(s) 170, 280
MvnI CGCG 2 cut(s) 249, 519
MwoI GCNNNNNNNGC 2 cut(s) 179, 399
NdeI CATATG 1 cut(s) 485
NdeII GATC 4 cut(s) 503, 514, 526, 891
NlaIII CATG 5 cut(s) 367, 555, 565, 680, 838
NlaIV GGNNCC 6 cut(s) 28, 149, 196, 353, 505, 577
NmeAIII GCCGAG 2 cut(s) 124, 199
NmuCI GTSAC 2 cut(s) 16, 448
NruI TCGCGA 1 cut(s) 519
PagI TCATGA 2 cut(s) 363, 676
PctI GAATGC 1 cut(s) 804
PfeI GAWTC 2 cut(s) 387, 476
PinAI ACCGGT 1 cut(s) 425
PkrI GCNGC 4 cut(s) 10, 181, 324, 600
PshBI ATTAAT 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 168, 278
PspGI CCWGG 2 cut(s) 168, 278
PspN4I GGNNCC 6 cut(s) 28, 149, 196, 353, 505, 577
PspPI GGNCC 1 cut(s) 282
PstI CTGCAG 1 cut(s) 600
PsuI RGATCY 2 cut(s) 503, 891
PvuII CAGCTG 2 cut(s) 601, 740
RruI TCGCGA 1 cut(s) 519
RsaI GTAC 7 cut(s) 196, 254, 623, 736, 762, 790, 884
RsaNI GTAC 7 cut(s) 195, 253, 622, 735, 761, 789, 883
SacI GAGCTC 1 cut(s) 363
SalI GTCGAC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 69, 222, 582
SatI GCNGC 4 cut(s) 9, 180, 323, 599
Sau3AI GATC 4 cut(s) 503, 514, 526, 891
Sau96I GGNCC 1 cut(s) 282
ScaI AGTACT 1 cut(s) 884
ScrFI CCNGG 2 cut(s) 170, 280
SduI GDGCHC 1 cut(s) 363
SfaNI GCATC 1 cut(s) 651
SfcI CTRYAG 2 cut(s) 59, 596
SfiI GGCCNNNNNGGCC 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 6 cut(s) 70, 225, 494, 583, 607, 769
SsiI CCGC 6 cut(s) 8, 179, 201, 259, 329, 391
SspI AATATT 1 cut(s) 67
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 168, 278
StyI CCWWGG 2 cut(s) 12, 730
TaaI ACNGT 2 cut(s) 119, 781
TaiI ACGT 1 cut(s) 693
TaqI TCGA 2 cut(s) 121, 378
TasI AATT 6 cut(s) 70, 225, 494, 583, 607, 769
TatI WGTACW 4 cut(s) 621, 760, 788, 882
TauI GCSGC 2 cut(s) 11, 182
TfiI GAWTC 2 cut(s) 387, 476
Tru1I TTAA 3 cut(s) 69, 222, 582
Tru9I TTAA 3 cut(s) 69, 222, 582
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 2 cut(s) 16, 448
TseI GCWGC 2 cut(s) 322, 598
Tsp45I GTSAC 2 cut(s) 16, 448
TspDTI ATGAA 2 cut(s) 660, 665
TspRI CASTG 1 cut(s) 544
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 3 cut(s) 225, 494, 607
XmiI GTMKAC 2 cut(s) 121, 726
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.