RLG00000005593

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016186
Physical Location & Seq
Reverse (-)
158491 .. 161017
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005593

Sequence Viewer

Length: 885 bp
ATGGCGATGGTTAGTGGCGCGGCCAACGGCGGGTTTAGAATTGATTCGGAGGAACATTCACGGAAGAAGAAAACGATGTTTCCCTATTCGTTTTCCCTGTTTCATCAGGTAACCGTACAGCCACCTCGCCGTCACCGACTAATAATAGTATGTGCAAGCTCCGATGGGGCGAAAGGTTCAGGGACCACAGCGAGAGGACGGAGACTCGTCAAAATTCGTGAAGATAAGAGGAAGCGCGAGTACGAGCGCCTTCACAACTATCCTGCTTGGGCCAAAGTGTTAGAAAACGCCTGCAAAGACGATGCTGAGCTTAGAGCCCTTCTGGGAGATAGCATTGGAAACCCTGAGCTCATGAGGAAAAGGGTTGAACAAAGAGTTCGCCAGAAGGGTAGAGACTTCCGCAAGTCCAAAACCGGTTCACTCCTTGCCTTCAAAGTCTCCTTCAGAGACTTCAACCCCCTTGATTCCTACATTTGGTTTGAATTCTTTGGATCACCGTCTGATCGTGATATTGATCTCTTTGGTAGTGTTATTCAGTCATGGTATGTTATGGGACGCTTGGGTGCCTTTAATTCTACTAATCTGCAGTTGGCAAATTCATCTATGGAGTACAATCCCCTTTATGATGTGGATAAGGGTTTCAAAGTGATGCCATCTTCATTTCATGAGATCAGTGACATTGAGTTCCAGGATAACTGGGGTCGTGTTTGGGTAGACCTGGGTACAGCTGACTATTTTGCTGTTGATGTACTGCTCAACTGCTTGACAGTCTTAAGTTCAGAATATATAGGCATTCAACAGATAGTTTTTGGTGGTCAGAGAATCGGTGATTGGGAAGAGGGGATGACCAACGCAGGGTATGGATATAAGTACTTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

295

Amino Acids

33.67

Weight (kDa)

9.09

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 145 - 284 8.8e-53 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08400 AT5G08400
fragaria_vesca FvH4_7g04330 FvH4_7g04330 FvH4_7g04330
malus_domestica MD16G1005400.v1.1
prunus_persica Prupe.1G352300_v2.0.a1
pyrus_communis pycom2749g00040
rosa_chinensis RchiOBHm_Chr4g0447251
rosa_laevigata RLG00000005593
rosa_multiflora Rmu_ssc0000396.1_g000003
rosa_roxburghii Rroxscaffold_5G00387610
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4DG448100
rosa_wichuraiana Rw4G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 563
AccI GTMKAC 1 cut(s) 714
AccII CGCG 2 cut(s) 20, 237
AciI CCGC 3 cut(s) 20, 30, 400
AclWI GGATC 1 cut(s) 499
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 3 cut(s) 213, 482, 595
AcuI CTGAAG 1 cut(s) 427
AfaI GTAC 6 cut(s) 117, 242, 611, 724, 750, 872
AfiI CCNNNNNNNGG 3 cut(s) 30, 474, 855
AflII CTTAAG 1 cut(s) 772
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 7 cut(s) 368, 433, 454, 482, 643, 797, 877
AjnI CCWGG 2 cut(s) 687, 717
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AluBI AGCT 4 cut(s) 159, 310, 349, 728
AluI AGCT 4 cut(s) 159, 310, 349, 728
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 4 cut(s) 196, 387, 441, 442
AlwI GGATC 1 cut(s) 499
AoxI GGCC 2 cut(s) 21, 270
ApoI RAATTY 3 cut(s) 213, 482, 595
AsiGI ACCGGT 1 cut(s) 413
Asp700I GAANNNNTTC 1 cut(s) 43
AspLEI GCGC 3 cut(s) 20, 237, 249
AspS9I GGNCC 2 cut(s) 183, 270
AsuHPI GGTGA 3 cut(s) 125, 486, 839
AvaII GGWCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 563
BanII GRGCYC 2 cut(s) 319, 351
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
Bbv12I GWGCWC 1 cut(s) 351
BccI CCATC 2 cut(s) 158, 661
BceAI ACGGC 2 cut(s) 43, 114
BciT130I CCWGG 2 cut(s) 689, 719
BcoDI GTCTC 4 cut(s) 196, 387, 441, 442
BfmI CTRYAG 1 cut(s) 584
BfoI RGCGCY 1 cut(s) 250
BfrI CTTAAG 1 cut(s) 772
BisI GCNGC 1 cut(s) 21
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 22
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 2 cut(s) 689, 719
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 183, 270
BmiI GGNNCC 2 cut(s) 184, 565
BmrFI CCNGG 2 cut(s) 689, 719
BmrI ACTGGG 1 cut(s) 706
BmsI GCATC 2 cut(s) 292, 639
BmuI ACTGGG 1 cut(s) 706
Bpu10I CCTNAGC 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 513
BsaJI CCNNGG 1 cut(s) 718
BsaWI WCCGGW 1 cut(s) 413
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 474, 855
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 701
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 2 cut(s) 689, 719
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 1 cut(s) 849
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 3 cut(s) 30, 474, 855
BseMII CTCAG 2 cut(s) 297, 336
BseNI ACTGG 1 cut(s) 701
Bsh1236I CGCG 2 cut(s) 20, 237
BshFI GGCC 2 cut(s) 23, 272
BshNI GGYRCC 1 cut(s) 563
BshTI ACCGGT 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 1 cut(s) 414
BslFI GGGAC 2 cut(s) 196, 567
BslI CCNNNNNNNGG 3 cut(s) 30, 474, 855
BsmAI GTCTC 4 cut(s) 196, 387, 441, 442
BsmFI GGGAC 2 cut(s) 196, 567
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 2 cut(s) 23, 272
Bsp1286I GDGCHC 2 cut(s) 319, 351
Bsp143I GATC 4 cut(s) 491, 502, 514, 669
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 3 cut(s) 20, 30, 400
BspANI GGCC 2 cut(s) 23, 272
BspCNI CTCAG 2 cut(s) 298, 337
BspFNI CGCG 2 cut(s) 20, 237
BspHI TCATGA 2 cut(s) 351, 664
BspLI GGNNCC 2 cut(s) 184, 565
BspMAI CTGCAG 1 cut(s) 588
BspPI GGATC 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 563
BspTI CTTAAG 1 cut(s) 772
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 1 cut(s) 701
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 4 cut(s) 491, 502, 514, 669
Bst2UI CCWGG 2 cut(s) 689, 719
Bst4CI ACNGT 3 cut(s) 115, 498, 769
Bst6I CTCTTC 1 cut(s) 831
BstAFI CTTAAG 1 cut(s) 772
BstC8I GCNNGC 2 cut(s) 157, 292
BstDEI CTNAG 3 cut(s) 306, 311, 345
BstEII GGTNACC 1 cut(s) 109
BstF5I GGATG 1 cut(s) 849
BstFNI CGCG 2 cut(s) 20, 237
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 3 cut(s) 20, 237, 249
BstKTI GATC 4 cut(s) 494, 505, 517, 672
BstMAI GTCTC 4 cut(s) 196, 387, 441, 442
BstMBI GATC 4 cut(s) 491, 502, 514, 669
BstNI CCWGG 2 cut(s) 689, 719
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 687, 717
BstSFI CTRYAG 1 cut(s) 584
BstUI CGCG 2 cut(s) 20, 237
BsuRI GGCC 2 cut(s) 23, 272
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 849
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 2 cut(s) 157, 292
CciI TCATGA 2 cut(s) 351, 664
CfoI GCGC 3 cut(s) 20, 237, 249
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 183, 270
CseI GACGC 1 cut(s) 564
Csp6I GTAC 6 cut(s) 116, 241, 610, 723, 749, 871
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 3 cut(s) 352, 540, 665
CviJI RGCY 8 cut(s) 23, 121, 159, 272, 310, 317, 349, 728
CviKI_1 RGCY 8 cut(s) 23, 121, 159, 272, 310, 317, 349, 728
CviQI GTAC 6 cut(s) 116, 241, 610, 723, 749, 871
DdeI CTNAG 3 cut(s) 306, 311, 345
DpnI GATC 4 cut(s) 493, 504, 516, 671
DpnII GATC 4 cut(s) 491, 502, 514, 669
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
Ecl136II GAGCTC 1 cut(s) 349
Eco24I GRGCYC 2 cut(s) 319, 351
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 427
Eco91I GGTNACC 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 687, 717
EcoT38I GRGCYC 2 cut(s) 319, 351
FaeI CATG 3 cut(s) 355, 543, 668
FaqI GGGAC 2 cut(s) 196, 567
FatI CATG 3 cut(s) 351, 539, 664
FauI CCCGC 1 cut(s) 23
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 856
FriOI GRGCYC 2 cut(s) 319, 351
Fsp4HI GCNGC 1 cut(s) 21
GlaI GCGC 3 cut(s) 19, 236, 248
GluI GCNGC 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 250
HaeIII GGCC 2 cut(s) 23, 272
HapII CCGG 1 cut(s) 414
HgaI GACGC 1 cut(s) 564
HhaI GCGC 3 cut(s) 20, 237, 249
Hin1II CATG 3 cut(s) 355, 543, 668
Hin6I GCGC 3 cut(s) 18, 235, 247
HinP1I GCGC 3 cut(s) 18, 235, 247
HinfI GANTC 4 cut(s) 44, 204, 464, 822
HpaII CCGG 1 cut(s) 414
HphI GGTGA 3 cut(s) 125, 486, 839
Hpy166II GTNNAC 2 cut(s) 419, 715
Hpy188I TCNGA 6 cut(s) 49, 163, 446, 502, 781, 819
Hpy188III TCNNGA 5 cut(s) 218, 352, 506, 665, 877
Hpy8I GTNNAC 2 cut(s) 419, 715
HpyAV CCTTC 5 cut(s) 260, 329, 379, 439, 451
HpyCH4III ACNGT 3 cut(s) 115, 498, 769
HpyCH4V TGCA 3 cut(s) 155, 294, 586
HpyF3I CTNAG 3 cut(s) 306, 311, 345
Hsp92II CATG 3 cut(s) 355, 543, 668
HspAI GCGC 3 cut(s) 18, 235, 247
Kzo9I GATC 4 cut(s) 491, 502, 514, 669
LmnI GCTCC 1 cut(s) 164
LweI GCATC 2 cut(s) 292, 639
MaeIII GTNAC 3 cut(s) 109, 131, 674
MalI GATC 4 cut(s) 493, 504, 516, 671
MboI GATC 4 cut(s) 491, 502, 514, 669
MboII GAAGA 5 cut(s) 76, 79, 233, 648, 848
MhlI GDGCHC 2 cut(s) 319, 351
MluCI AATT 5 cut(s) 39, 213, 482, 571, 595
MlyI GAGTC 1 cut(s) 198
MnlI CCTC 6 cut(s) 43, 135, 188, 222, 348, 832
MroXI GAANNNNTTC 1 cut(s) 43
MseI TTAA 3 cut(s) 570, 773, 883
MspA1I CMGCKG 1 cut(s) 728
MspCI CTTAAG 1 cut(s) 772
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 2 cut(s) 689, 719
Mva1269I GAATGC 1 cut(s) 792
MvaI CCWGG 2 cut(s) 689, 719
MvnI CGCG 2 cut(s) 20, 237
NdeII GATC 4 cut(s) 491, 502, 514, 669
NlaIII CATG 3 cut(s) 355, 543, 668
NlaIV GGNNCC 2 cut(s) 184, 565
NmuCI GTSAC 2 cut(s) 131, 674
PagI TCATGA 2 cut(s) 351, 664
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PctI GAATGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 43
PfeI GAWTC 3 cut(s) 44, 464, 822
PfoI TCCNGGA 1 cut(s) 687
PinAI ACCGGT 1 cut(s) 413
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 687, 717
PspEI GGTNACC 1 cut(s) 109
PspGI CCWGG 2 cut(s) 687, 717
PspN4I GGNNCC 2 cut(s) 184, 565
PspPI GGNCC 2 cut(s) 183, 270
PstI CTGCAG 1 cut(s) 588
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 6 cut(s) 117, 242, 611, 724, 750, 872
RsaNI GTAC 6 cut(s) 116, 241, 610, 723, 749, 871
SacI GAGCTC 1 cut(s) 351
SaqAI TTAA 3 cut(s) 570, 773, 883
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 4 cut(s) 491, 502, 514, 669
Sau96I GGNCC 2 cut(s) 183, 270
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 2 cut(s) 689, 719
SduI GDGCHC 2 cut(s) 319, 351
SetI ASST 8 cut(s) 111, 127, 161, 178, 312, 351, 720, 730
SfaNI GCATC 2 cut(s) 292, 639
SfcI CTRYAG 1 cut(s) 584
SinI GGWCC 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 5 cut(s) 39, 213, 482, 571, 595
SsiI CCGC 3 cut(s) 20, 30, 400
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 687, 717
TaaI ACNGT 3 cut(s) 115, 498, 769
TasI AATT 5 cut(s) 39, 213, 482, 571, 595
TatI WGTACW 3 cut(s) 609, 748, 870
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 3 cut(s) 44, 464, 822
Tru1I TTAA 3 cut(s) 570, 773, 883
Tru9I TTAA 3 cut(s) 570, 773, 883
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 2 cut(s) 131, 674
Tsp45I GTSAC 2 cut(s) 131, 674
TspDTI ATGAA 4 cut(s) 92, 588, 648, 653
TspGWI ACGGA 2 cut(s) 76, 214
TspRI CASTG 1 cut(s) 679
Vha464I CTTAAG 1 cut(s) 772
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 3 cut(s) 213, 482, 595
XmiI GTMKAC 1 cut(s) 714
XmnI GAANNNNTTC 1 cut(s) 43
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.