Prupe.1G352300_v2.0.a1

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016186
Physical Location & Seq
Forward (+)
32752944 .. 32755788
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G352300.1

Sequence Viewer

Length: 948 bp
ATGAAGGCGGCCATAGCCACAGGAGCCACAGCCCAGGGCGGGTTTATACTCCCGTATAATAATAGTAGTAGAACTGAAGAATCCCACTGTCATTCATGGAAGAAGAAAACGGTCTATCCCAATCCCAATTCCTATCTGCCAAAGCCACCTCGGCTCTTATTACTAGTATGTGCCACTGCCAATGGCACTAATACCAGTACCAATGATCTGGGTCTGGGCGGCGGTGGTGGTGCCAAAGGCTCAGGAACCACTGCCAGAGGACGCAGACTTCTTAAAATTCGTGAAGAGAAGAGGAAACGCGAGCACGATCGCCTTCACAACTACCCTGCCTGGGCCAAAGTGTTGGAAGACGCCTGCAAAGACGATGCTGAGCTGCGAGCGGTTCTTGGAGATAGCATTGGCAACCCTGAGCTCATGAGGAAAAGGGTTGAAGAAAGAGTTCGCAAGAAGGGTAGGGACTTCAGCAAATCCAAAACCGGTTCTGTCCTTGCCTTCAAAGTCACCTTCAGAGACTTCAATCCTCTTGATTCCTACATATGGTTTGAATTCTATGGATCGCCATCTGATCGCGACGTCGAGCTCTTTGGCAGTGTTATTCAGTCATGGTATGTTATGGGACGCTTGGGTGCCTTTAATTCTACTAATCTGCAGCTTGCAAATTCTTCCATGGAGTATAATCCCCTTTATGATGTGGATAAGGGTTTCAAAGTGATGCCATCTTCATTTCATGATATCAGTGACGTTGAGTTTCAGGATAACTGGGCTCGTGTTTGGGTAGACCTTGGTACAGCTGACTATTTTGCCATGGATGTGCTCCTCAACTGCTTGACCGTCTTGAGCTCAGAATATCTAGGCATTCAACAGATAGTTTTTGGTGGGCATAGAATTGGTGATTGGGAAGAGGGGATGACCAACACAGAGGATGGATATAAGTATTTCAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

316

Amino Acids

35.42

Weight (kDa)

8.21

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08400 AT5G08400
fragaria_vesca FvH4_7g04330 FvH4_7g04330 FvH4_7g04330
malus_domestica MD16G1005400.v1.1
prunus_persica Prupe.1G352300_v2.0.a1
pyrus_communis pycom2749g00040
rosa_chinensis RchiOBHm_Chr4g0447251
rosa_laevigata RLG00000005593
rosa_multiflora Rmu_ssc0000396.1_g000003
rosa_roxburghii Rroxscaffold_5G00387610
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4DG448100
rosa_wichuraiana Rw4G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 576
AccB1I GGYRCC 2 cut(s) 230, 626
AccBSI CCGCTC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 777
AccII CGCG 2 cut(s) 300, 570
AciI CCGC 5 cut(s) 8, 39, 219, 222, 380
AclWI GGATC 1 cut(s) 562
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 3 cut(s) 276, 545, 658
AcuI CTGAAG 3 cut(s) 96, 445, 490
AcyI GRCGYC 2 cut(s) 351, 573
AfaI GTAC 2 cut(s) 199, 787
AfiI CCNNNNNNNGG 3 cut(s) 39, 331, 537
AgeI ACCGGT 1 cut(s) 476
AgsI TTSAA 7 cut(s) 431, 496, 517, 545, 706, 860, 940
AhlI ACTAGT 1 cut(s) 163
AjnI CCWGG 2 cut(s) 33, 329
AjuI GAANNNNNNNTTGG 2 cut(s) 877, 909
AluBI AGCT 6 cut(s) 373, 412, 580, 652, 791, 840
AluI AGCT 6 cut(s) 373, 412, 580, 652, 791, 840
Alw21I GWGCWC 5 cut(s) 306, 414, 582, 816, 842
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 1 cut(s) 562
AoxI GGCC 2 cut(s) 9, 333
ApeKI GCWGC 2 cut(s) 373, 649
ApoI RAATTY 3 cut(s) 276, 545, 658
AsiGI ACCGGT 1 cut(s) 476
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 2 cut(s) 493, 902
BanI GGYRCC 2 cut(s) 230, 626
BanII GRGCYC 4 cut(s) 414, 582, 766, 842
BauI CACGAG 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 5 cut(s) 306, 414, 582, 816, 842
BbvI GCAGC 2 cut(s) 360, 661
BccI CCATC 3 cut(s) 568, 724, 917
BciT130I CCWGG 2 cut(s) 35, 331
BcoDI GTCTC 1 cut(s) 504
BcuI ACTAGT 1 cut(s) 163
BfaI CTAG 2 cut(s) 164, 851
BfmI CTRYAG 1 cut(s) 647
BglI GCCNNNNNGGC 1 cut(s) 151
BglII AGATCT 1 cut(s) 942
BisI GCNGC 4 cut(s) 9, 220, 374, 650
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 4 cut(s) 10, 221, 375, 651
Bme1390I CCNGG 2 cut(s) 35, 331
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 4 cut(s) 25, 232, 247, 628
BmrFI CCNGG 2 cut(s) 35, 331
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 2 cut(s) 355, 702
BmuI ACTGGG 1 cut(s) 769
BpiI GAAGAC 1 cut(s) 354
Bpu10I CCTNAGC 2 cut(s) 241, 408
Bpu1102I GCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 856
BsaBI GATNNNNATC 1 cut(s) 559
BsaHI GRCGYC 2 cut(s) 351, 573
BsaJI CCNNGG 7 cut(s) 33, 34, 149, 330, 666, 781, 804
BsaWI WCCGGW 1 cut(s) 476
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 331, 537
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 2 cut(s) 195, 764
Bse8I GATNNNNATC 1 cut(s) 559
BseBI CCWGG 2 cut(s) 35, 331
BseDI CCNNGG 7 cut(s) 33, 34, 149, 330, 666, 781, 804
BseGI GGATG 3 cut(s) 814, 912, 928
BseJI GATNNNNATC 1 cut(s) 559
BseLI CCNNNNNNNGG 3 cut(s) 39, 331, 537
BseMII CTCAG 4 cut(s) 255, 360, 399, 855
BseNI ACTGG 2 cut(s) 195, 764
BseRI GAGGAG 1 cut(s) 806
BseXI GCAGC 2 cut(s) 360, 661
Bsh1236I CGCG 2 cut(s) 300, 570
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 2 cut(s) 11, 335
BshNI GGYRCC 2 cut(s) 230, 626
BshTI ACCGGT 1 cut(s) 476
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 5 cut(s) 306, 414, 582, 816, 842
BsiSI CCGG 1 cut(s) 477
BslFI GGGAC 2 cut(s) 470, 630
BslI CCNNNNNNNGG 3 cut(s) 39, 331, 537
BsmAI GTCTC 1 cut(s) 504
BsmFI GGGAC 2 cut(s) 470, 630
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 2 cut(s) 11, 335
Bsp1286I GDGCHC 6 cut(s) 306, 414, 582, 766, 816, 842
Bsp143I GATC 5 cut(s) 205, 307, 554, 565, 942
Bsp1720I GCTNAGC 1 cut(s) 369
Bsp19I CCATGG 2 cut(s) 666, 804
Bsp68I TCGCGA 1 cut(s) 570
BspACI CCGC 5 cut(s) 8, 39, 219, 222, 380
BspANI GGCC 2 cut(s) 11, 335
BspCNI CTCAG 4 cut(s) 254, 361, 400, 854
BspFNI CGCG 2 cut(s) 300, 570
BspHI TCATGA 2 cut(s) 414, 727
BspLI GGNNCC 4 cut(s) 25, 232, 247, 628
BspMAI CTGCAG 1 cut(s) 651
BspPI GGATC 1 cut(s) 562
BspT107I GGYRCC 2 cut(s) 230, 626
BsrBI CCGCTC 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 476
BsrI ACTGG 2 cut(s) 195, 764
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 7 cut(s) 33, 34, 149, 330, 666, 781, 804
BssMI GATC 5 cut(s) 205, 307, 554, 565, 942
BssNI GRCGYC 2 cut(s) 351, 573
BssSI CACGAG 1 cut(s) 765
BssT1I CCWWGG 3 cut(s) 666, 781, 804
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 2 cut(s) 35, 331
Bst4CI ACNGT 3 cut(s) 89, 112, 832
Bst6I CTCTTC 3 cut(s) 279, 284, 894
BstACI GRCGYC 2 cut(s) 351, 573
BstC8I GCNNGC 4 cut(s) 302, 355, 378, 654
BstDEI CTNAG 4 cut(s) 241, 369, 408, 841
BstDSI CCRYGG 2 cut(s) 666, 804
BstF5I GGATG 3 cut(s) 814, 912, 928
BstFNI CGCG 2 cut(s) 300, 570
BstKTI GATC 5 cut(s) 208, 310, 557, 568, 945
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 5 cut(s) 205, 307, 554, 565, 942
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 14, 23, 151
BstNI CCWGG 2 cut(s) 35, 331
BstSCI CCNGG 2 cut(s) 33, 329
BstSFI CTRYAG 1 cut(s) 647
BstUI CGCG 2 cut(s) 300, 570
BstV1I GCAGC 2 cut(s) 360, 661
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 942
BstXI CCANNNNNNTGG 2 cut(s) 208, 343
BstYI RGATCY 1 cut(s) 942
BsuRI GGCC 2 cut(s) 11, 335
BtgI CCRYGG 2 cut(s) 666, 804
BtsCI GGATG 3 cut(s) 814, 912, 928
BtsI GCAGTG 3 cut(s) 174, 249, 595
BtsIMutI CAGTG 5 cut(s) 85, 174, 249, 595, 742
BtuMI TCGCGA 1 cut(s) 570
Cac8I GCNNGC 4 cut(s) 302, 355, 378, 654
CciI TCATGA 2 cut(s) 414, 727
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 1 cut(s) 333
CseI GACGC 3 cut(s) 270, 359, 627
Csp6I GTAC 2 cut(s) 198, 786
CspAI ACCGGT 1 cut(s) 476
CviAII CATG 6 cut(s) 96, 415, 603, 667, 728, 805
CviQI GTAC 2 cut(s) 198, 786
DdeI CTNAG 4 cut(s) 241, 369, 408, 841
DpnI GATC 5 cut(s) 207, 309, 556, 567, 944
DpnII GATC 5 cut(s) 205, 307, 554, 565, 942
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 3 cut(s) 279, 284, 894
EarI CTCTTC 3 cut(s) 279, 284, 894
Ecl136II GAGCTC 3 cut(s) 412, 580, 840
Eco130I CCWWGG 3 cut(s) 666, 781, 804
Eco24I GRGCYC 4 cut(s) 414, 582, 766, 842
Eco32I GATATC 1 cut(s) 733
Eco53kI GAGCTC 3 cut(s) 412, 580, 840
Eco57I CTGAAG 3 cut(s) 96, 445, 490
EcoICRI GAGCTC 3 cut(s) 412, 580, 840
EcoRI GAATTC 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 33, 329
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 3 cut(s) 666, 781, 804
EcoT38I GRGCYC 4 cut(s) 414, 582, 766, 842
ErhI CCWWGG 3 cut(s) 666, 781, 804
FaeI CATG 6 cut(s) 99, 418, 606, 670, 731, 808
FaqI GGGAC 2 cut(s) 470, 630
FatI CATG 6 cut(s) 95, 414, 602, 666, 727, 804
FauI CCCGC 1 cut(s) 32
FauNDI CATATG 1 cut(s) 536
FblI GTMKAC 1 cut(s) 777
Fnu4HI GCNGC 4 cut(s) 9, 220, 374, 650
FokI GGATG 3 cut(s) 821, 919, 935
FriOI GRGCYC 4 cut(s) 414, 582, 766, 842
Fsp4HI GCNGC 4 cut(s) 9, 220, 374, 650
FspBI CTAG 2 cut(s) 164, 851
GluI GCNGC 4 cut(s) 9, 220, 374, 650
HaeIII GGCC 2 cut(s) 11, 335
HapII CCGG 1 cut(s) 477
HgaI GACGC 3 cut(s) 270, 359, 627
Hin1I GRCGYC 2 cut(s) 351, 573
Hin1II CATG 6 cut(s) 99, 418, 606, 670, 731, 808
HinfI GANTC 2 cut(s) 80, 527
HpaII CCGG 1 cut(s) 477
HphI GGTGA 2 cut(s) 493, 902
Hpy166II GTNNAC 1 cut(s) 778
Hpy188I TCNGA 4 cut(s) 509, 565, 844, 947
Hpy188III TCNNGA 9 cut(s) 243, 281, 415, 524, 569, 728, 752, 835, 940
Hpy8I GTNNAC 1 cut(s) 778
Hpy99I CGWCG 2 cut(s) 575, 578
HpyAV CCTTC 4 cut(s) 323, 442, 502, 514
HpyCH4III ACNGT 3 cut(s) 89, 112, 832
HpyCH4IV ACGT 2 cut(s) 573, 741
HpyCH4V TGCA 3 cut(s) 357, 649, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 23, 151
HpyF3I CTNAG 4 cut(s) 241, 369, 408, 841
HpySE526I ACGT 2 cut(s) 573, 741
Hsp92I GRCGYC 2 cut(s) 351, 573
Hsp92II CATG 6 cut(s) 99, 418, 606, 670, 731, 808
Kzo9I GATC 5 cut(s) 205, 307, 554, 565, 942
LmnI GCTCC 2 cut(s) 23, 819
Lsp1109I GCAGC 2 cut(s) 360, 661
LweI GCATC 2 cut(s) 355, 702
MaeI CTAG 2 cut(s) 164, 851
MaeII ACGT 2 cut(s) 573, 741
MaeIII GTNAC 2 cut(s) 499, 737
MalI GATC 5 cut(s) 207, 309, 556, 567, 944
MbiI CCGCTC 1 cut(s) 380
MboI GATC 5 cut(s) 205, 307, 554, 565, 942
MflI RGATCY 1 cut(s) 942
MhlI GDGCHC 6 cut(s) 306, 414, 582, 766, 816, 842
MluCI AATT 6 cut(s) 127, 276, 545, 634, 658, 885
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 8 cut(s) 159, 251, 285, 411, 531, 827, 895, 913
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 2 cut(s) 273, 633
MslI CAYNNNNRTG 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 2 cut(s) 35, 331
Mva1269I GAATGC 1 cut(s) 855
MvaI CCWGG 2 cut(s) 35, 331
MvnI CGCG 2 cut(s) 300, 570
MwoI GCNNNNNNNGC 3 cut(s) 14, 23, 151
NcoI CCATGG 2 cut(s) 666, 804
NdeI CATATG 1 cut(s) 536
NdeII GATC 5 cut(s) 205, 307, 554, 565, 942
NlaIII CATG 6 cut(s) 99, 418, 606, 670, 731, 808
NlaIV GGNNCC 4 cut(s) 25, 232, 247, 628
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 499, 737
NruI TCGCGA 1 cut(s) 570
PagI TCATGA 2 cut(s) 414, 727
PasI CCCWGGG 1 cut(s) 34
PctI GAATGC 1 cut(s) 855
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 2 cut(s) 80, 527
PinAI ACCGGT 1 cut(s) 476
PkrI GCNGC 4 cut(s) 10, 221, 375, 651
Ple19I CGATCG 1 cut(s) 310
Psp124BI GAGCTC 3 cut(s) 414, 582, 842
Psp6I CCWGG 2 cut(s) 33, 329
PspGI CCWGG 2 cut(s) 33, 329
PspN4I GGNNCC 4 cut(s) 25, 232, 247, 628
PspPI GGNCC 1 cut(s) 333
PstI CTGCAG 1 cut(s) 651
PsuI RGATCY 1 cut(s) 942
PvuI CGATCG 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 791
RruI TCGCGA 1 cut(s) 570
RsaI GTAC 2 cut(s) 199, 787
RsaNI GTAC 2 cut(s) 198, 786
RseI CAYNNNNRTG 1 cut(s) 809
SacI GAGCTC 3 cut(s) 414, 582, 842
SaqAI TTAA 2 cut(s) 273, 633
SatI GCNGC 4 cut(s) 9, 220, 374, 650
Sau3AI GATC 5 cut(s) 205, 307, 554, 565, 942
Sau96I GGNCC 1 cut(s) 333
ScrFI CCNGG 2 cut(s) 35, 331
SduI GDGCHC 6 cut(s) 306, 414, 582, 766, 816, 842
SfaNI GCATC 2 cut(s) 355, 702
SfcI CTRYAG 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 809
SmlI CTYRAG 1 cut(s) 835
SmoI CTYRAG 1 cut(s) 835
SpeI ACTAGT 1 cut(s) 163
Sse9I AATT 6 cut(s) 127, 276, 545, 634, 658, 885
SsiI CCGC 5 cut(s) 8, 39, 219, 222, 380
SspMI CTAG 2 cut(s) 164, 851
SstI GAGCTC 3 cut(s) 414, 582, 842
StyD4I CCNGG 2 cut(s) 33, 329
StyI CCWWGG 3 cut(s) 666, 781, 804
TaaI ACNGT 3 cut(s) 89, 112, 832
TaiI ACGT 2 cut(s) 576, 744
TaqI TCGA 1 cut(s) 576
TasI AATT 6 cut(s) 127, 276, 545, 634, 658, 885
TauI GCSGC 2 cut(s) 11, 222
TfiI GAWTC 2 cut(s) 80, 527
Tru1I TTAA 2 cut(s) 273, 633
Tru9I TTAA 2 cut(s) 273, 633
TscAI CASTG 5 cut(s) 92, 181, 256, 595, 742
TseFI GTSAC 2 cut(s) 499, 737
TseI GCWGC 2 cut(s) 373, 649
Tsp45I GTSAC 2 cut(s) 499, 737
TspDTI ATGAA 4 cut(s) 17, 84, 711, 716
TspRI CASTG 5 cut(s) 92, 181, 256, 595, 742
XapI RAATTY 3 cut(s) 276, 545, 658
XmiI GTMKAC 1 cut(s) 777
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 2 cut(s) 164, 851
ZraI GACGTC 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.