FvH4_7g04330

Protein of unknown function (DUF3531)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016186
Physical Location & Seq
Forward (+)
4911100 .. 4915131
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04330.t2

Sequence Viewer

Length: 897 bp
ATGAAGGCGACCATGGCCATGGGCATAGGGGCAGCCAACGGCACGTTTAGAACTGATTGGGAGGATCATTCATGGAACAGGAAAACAATGTTGCCATTGCCGTATACCTATTTTCATGTAACACTACAGCCACCTCGTCATCACCAACTAATAGTATGTTCAAGCTTGGAGTCGGATGGGGCAAAAGGCTCAGGAACCACAGCCAGAGGACGCAGACTTCTTAAAATCCGTGAAGATAAGAGAAGGCGCGAGTACGAACGCCTTCACAACTATCCTGCTTGGGCCAAAGTGTTAGAAAATGCGTGCAAAGATGATGCTGAGCTTAGAGCCCTTCTGGGAGATAGCATCGGAAACCCTGAGCTCATGAGGAAAAGGGTTGAAGAAAGAGTTCGCCAGAAGGGCAGAGACTTCCGCAAATCAAAAACTGGTTCAGTCCTTGCCTTCAAAGTCTCCTTCAGAGACTTCAATCCCCTTGATTCTTACATTTGGTTTGAATTCTTTGGATCACTGTCTGATCGTGATGTTGATCTCTTTGGAAGTGTTATTCAGTCATGGTATGTTATGGGACGCTTGGGTGCCTTTAATTCTACTAATCTGCAGTTGGCAAATTCATCTATGGAGTACAATCCCCTCTATGATGTGGATAAGGGTTTCAAAGTGATGCCATCTTCATTTCATGAGATCAGTGACATTGAGTTTCAAGATAACTGGGGTCGTGTTTGGGTAGACCTGGGTACAGCTGACTATTTTGCTGTTGATGTACTCCTCAACTGCTTGACAGTCTTAAGTTCAGAATATATAGGTATTCAGCAGATAGTTTTTGGTGGTCAGAGAATCGGCGATTGGGAAGAGGGAATGACCAACGCAGAGTATGGATATAAGTACTTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3531

Protein Analysis

299

Amino Acids

34.34

Weight (kDa)

7.02

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3531 PF12049 149 - 288 4.1e-52 Protein of unknown function (DUF3531)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08400 AT5G08400
fragaria_vesca FvH4_7g04330 FvH4_7g04330 FvH4_7g04330
malus_domestica MD16G1005400.v1.1
prunus_persica Prupe.1G352300_v2.0.a1
pyrus_communis pycom2749g00040
rosa_chinensis RchiOBHm_Chr4g0447251
rosa_laevigata RLG00000005593
rosa_multiflora Rmu_ssc0000396.1_g000003
rosa_roxburghii Rroxscaffold_5G00387610
rosa_rugosa Rorug04G0379000
rosa_samantha Rh4DG448100
rosa_wichuraiana Rw4G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 575
AccI GTMKAC 2 cut(s) 104, 726
AccII CGCG 1 cut(s) 249
AciI CCGC 1 cut(s) 412
AclWI GGATC 2 cut(s) 72, 511
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 2 cut(s) 494, 607
AcuI CTGAAG 1 cut(s) 439
AfaI GTAC 5 cut(s) 254, 623, 736, 762, 884
AflII CTTAAG 1 cut(s) 784
AgsI TTSAA 8 cut(s) 162, 380, 445, 466, 494, 655, 701, 889
AjnI CCWGG 1 cut(s) 729
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
AluBI AGCT 4 cut(s) 165, 322, 361, 740
AluI AGCT 4 cut(s) 165, 322, 361, 740
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 3 cut(s) 399, 453, 454
AlwI GGATC 2 cut(s) 72, 511
AoxI GGCC 2 cut(s) 15, 282
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 2 cut(s) 494, 607
Asp700I GAANNNNTTC 2 cut(s) 261, 387
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 134
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 2 cut(s) 331, 363
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 1 cut(s) 44
BccI CCATC 2 cut(s) 170, 673
BceAI ACGGC 2 cut(s) 55, 85
BciT130I CCWGG 1 cut(s) 731
BcoDI GTCTC 3 cut(s) 399, 453, 454
BfmI CTRYAG 2 cut(s) 125, 596
BfrI CTTAAG 1 cut(s) 784
BglI GCCNNNNNGGC 1 cut(s) 399
BisI GCNGC 1 cut(s) 33
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 34
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 1 cut(s) 731
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 196, 577
BmrFI CCNGG 1 cut(s) 731
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 3 cut(s) 304, 354, 651
BmuI ACTGGG 1 cut(s) 718
Bpu10I CCTNAGC 2 cut(s) 190, 357
Bpu1102I GCTNAGC 1 cut(s) 318
BsaBI GATNNNNATC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 12, 18, 730
Bse1I ACTGG 2 cut(s) 430, 713
Bse3DI GCAATG 1 cut(s) 95
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 3 cut(s) 12, 18, 730
BseGI GGATG 1 cut(s) 181
BseJI GATNNNNATC 1 cut(s) 525
BseMI GCAATG 1 cut(s) 95
BseMII CTCAG 3 cut(s) 204, 309, 348
BseNI ACTGG 2 cut(s) 430, 713
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 2 cut(s) 17, 284
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 1 cut(s) 579
BsmAI GTCTC 3 cut(s) 399, 453, 454
BsmFI GGGAC 1 cut(s) 579
BsnI GGCC 2 cut(s) 17, 284
Bsp1286I GDGCHC 2 cut(s) 331, 363
Bsp143I GATC 5 cut(s) 64, 503, 514, 526, 681
Bsp1720I GCTNAGC 1 cut(s) 318
Bsp19I CCATGG 2 cut(s) 12, 18
BspACI CCGC 1 cut(s) 412
BspANI GGCC 2 cut(s) 17, 284
BspCNI CTCAG 3 cut(s) 203, 310, 349
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 2 cut(s) 363, 676
BspLI GGNNCC 2 cut(s) 196, 577
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 2 cut(s) 72, 511
BspT107I GGYRCC 1 cut(s) 575
BspTI CTTAAG 1 cut(s) 784
BsrDI GCAATG 1 cut(s) 95
BsrI ACTGG 2 cut(s) 430, 713
BssECI CCNNGG 3 cut(s) 12, 18, 730
BssMI GATC 5 cut(s) 64, 503, 514, 526, 681
BssNAI GTATAC 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 12, 18
Bst1107I GTATAC 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 2 cut(s) 510, 781
Bst6I CTCTTC 1 cut(s) 843
BstAFI CTTAAG 1 cut(s) 784
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 4 cut(s) 190, 318, 323, 357
BstDSI CCRYGG 2 cut(s) 12, 18
BstF5I GGATG 1 cut(s) 181
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 5 cut(s) 67, 506, 517, 529, 684
BstMAI GTCTC 3 cut(s) 399, 453, 454
BstMBI GATC 5 cut(s) 64, 503, 514, 526, 681
BstMWI GCNNNNNNNGC 2 cut(s) 14, 399
BstNI CCWGG 1 cut(s) 731
BstSCI CCNGG 1 cut(s) 729
BstSFI CTRYAG 2 cut(s) 125, 596
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 44
BstXI CCANNNNNNTGG 1 cut(s) 19
BstZ17I GTATAC 1 cut(s) 105
BsuRI GGCC 2 cut(s) 17, 284
BtgI CCRYGG 2 cut(s) 12, 18
BtsCI GGATG 1 cut(s) 181
BtsIMutI CAGTG 2 cut(s) 506, 691
Cac8I GCNNGC 1 cut(s) 304
CciI TCATGA 2 cut(s) 363, 676
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 2 cut(s) 219, 576
Csp6I GTAC 5 cut(s) 253, 622, 735, 761, 883
CviAII CATG 7 cut(s) 13, 19, 72, 116, 364, 552, 677
CviQI GTAC 5 cut(s) 253, 622, 735, 761, 883
DdeI CTNAG 4 cut(s) 190, 318, 323, 357
DpnI GATC 5 cut(s) 66, 505, 516, 528, 683
DpnII GATC 5 cut(s) 64, 503, 514, 526, 681
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 2 cut(s) 12, 18
Eco24I GRGCYC 2 cut(s) 331, 363
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 729
EcoT14I CCWWGG 2 cut(s) 12, 18
EcoT38I GRGCYC 2 cut(s) 331, 363
ErhI CCWWGG 2 cut(s) 12, 18
FaeI CATG 7 cut(s) 16, 22, 75, 119, 367, 555, 680
FaqI GGGAC 1 cut(s) 579
FatI CATG 7 cut(s) 12, 18, 71, 115, 363, 551, 676
FblI GTMKAC 2 cut(s) 104, 726
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 2 cut(s) 331, 363
Fsp4HI GCNGC 1 cut(s) 33
GlaI GCGC 1 cut(s) 248
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 2 cut(s) 17, 284
HgaI GACGC 2 cut(s) 219, 576
HhaI GCGC 1 cut(s) 249
Hin1II CATG 7 cut(s) 16, 22, 75, 119, 367, 555, 680
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 3 cut(s) 170, 476, 834
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 105, 727
Hpy188I TCNGA 6 cut(s) 175, 350, 458, 514, 793, 831
Hpy188III TCNNGA 6 cut(s) 192, 364, 518, 677, 701, 889
Hpy8I GTNNAC 2 cut(s) 105, 727
HpyAV CCTTC 6 cut(s) 237, 272, 341, 391, 451, 463
HpyCH4III ACNGT 2 cut(s) 510, 781
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 2 cut(s) 306, 598
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 399
HpyF3I CTNAG 4 cut(s) 190, 318, 323, 357
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 7 cut(s) 16, 22, 75, 119, 367, 555, 680
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 5 cut(s) 64, 503, 514, 526, 681
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 3 cut(s) 304, 354, 651
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 118, 686
MalI GATC 5 cut(s) 66, 505, 516, 528, 683
MboI GATC 5 cut(s) 64, 503, 514, 526, 681
MboII GAAGA 4 cut(s) 245, 392, 660, 860
MhlI GDGCHC 2 cut(s) 331, 363
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 3 cut(s) 494, 583, 607
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 7 cut(s) 55, 144, 200, 360, 641, 776, 844
Mox20I TGGCCA 1 cut(s) 17
MroXI GAANNNNTTC 2 cut(s) 261, 387
MscI TGGCCA 1 cut(s) 17
MseI TTAA 4 cut(s) 222, 582, 785, 895
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 740
MspCI CTTAAG 1 cut(s) 784
MspR9I CCNGG 1 cut(s) 731
MvaI CCWGG 1 cut(s) 731
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 14, 399
NcoI CCATGG 2 cut(s) 12, 18
NdeII GATC 5 cut(s) 64, 503, 514, 526, 681
NlaIII CATG 7 cut(s) 16, 22, 75, 119, 367, 555, 680
NlaIV GGNNCC 2 cut(s) 196, 577
NmuCI GTSAC 1 cut(s) 686
PagI TCATGA 2 cut(s) 363, 676
PdmI GAANNNNTTC 2 cut(s) 261, 387
PfeI GAWTC 2 cut(s) 476, 834
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 729
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 2 cut(s) 196, 577
PspPI GGNCC 1 cut(s) 282
PstI CTGCAG 1 cut(s) 600
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 5 cut(s) 254, 623, 736, 762, 884
RsaNI GTAC 5 cut(s) 253, 622, 735, 761, 883
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 4 cut(s) 222, 582, 785, 895
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 5 cut(s) 64, 503, 514, 526, 681
Sau96I GGNCC 1 cut(s) 282
ScaI AGTACT 1 cut(s) 884
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 731
SduI GDGCHC 2 cut(s) 331, 363
SetI ASST 9 cut(s) 47, 110, 136, 167, 324, 363, 732, 742, 805
SfaNI GCATC 3 cut(s) 304, 354, 651
SfcI CTRYAG 2 cut(s) 125, 596
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
Sse9I AATT 3 cut(s) 494, 583, 607
SsiI CCGC 1 cut(s) 412
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 729
StyI CCWWGG 2 cut(s) 12, 18
TaaI ACNGT 2 cut(s) 510, 781
TaiI ACGT 1 cut(s) 47
TasI AATT 3 cut(s) 494, 583, 607
TatI WGTACW 3 cut(s) 621, 760, 882
TfiI GAWTC 2 cut(s) 476, 834
Tru1I TTAA 4 cut(s) 222, 582, 785, 895
Tru9I TTAA 4 cut(s) 222, 582, 785, 895
TscAI CASTG 2 cut(s) 513, 691
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 6 cut(s) 17, 60, 104, 600, 660, 665
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 513, 691
Vha464I CTTAAG 1 cut(s) 784
XapI RAATTY 2 cut(s) 494, 607
XmiI GTMKAC 2 cut(s) 104, 726
XmnI GAANNNNTTC 2 cut(s) 261, 387
ZrmI AGTACT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.