AT5G09430

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013156
Physical Location & Seq
Forward (+)
2931963 .. 2933423
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G09430.1

Sequence Viewer

Length: 936 bp
ATGTTTGATCATATGAAACTGCTGCGATGCTTCAGCTTCACGGCGTCGCGAGATTGGTTATTCCGGCAATCATTCGCAAACGCCGGTCTACGTTCCGTAACGACAGATCTCTCCCACGGCAATTCAATCGCATCAACAGCTATGCATTGTTGGATTCCTAAATCCCCGAATCGATCTAAACCTAATCTCCTCCTCCTCCACGGATTCGGCGCCAACGCAATGTGGCAATACGGAGAACATCTCCGAGCTTTCACCGGCCGATTCAACGTCTACGTTCCCGATCTCCTCTTCTTCGGGTTATCATCCACATCGGAACCAAACCGAACCGAATCTTTCCAGGCTCGGTGTCTGATGAGGTTAATGGAAGCGCACGGAGTCCAGAGGATGAACATCGTCGGGATTAGTTACGGTGGATTCGTTGGGTATAGTTTAGCGGCGCAGTTTCCGGAGAACGTTGAGAAGCTTGTGCTGTGCTGCGCAGGGGTTTGTTTGGAGGAGAAAGATATGGAGGACGGATTGTTTAAGGTGCCGAATCTAGAAGAAGCTACCGGAATTTTGATTCCTCAGACGCCGGAAAAGCTTAAGGAACTCATAAGATTCTCTTTCGTTAAACCAATCAAAGGTGTTCCTTCGTTTTTTCTCTGGGATTTTATCGATGTAATGTGTACGGAGTTTGTAGAGGAGAAGAGAGATTTGATCAAATCAATACTCAAAGATAGGAGACTTTCTGATCTTCCTAGGATCAAACAGAAATCGTTGATCATATGGGGAGAAGAAGATCAAATATTTCCACTGGAACTAGGTTACAGATTAAAAAGACATATAGGAGAGAGTGCAGAGATAGTGGTGATCAAGAAAGCAGGACATGCTGTGAATTTGGAGAAGTCCAAGGAATTTGTCAAGCATTTGAAATCTTTTCTCATTGACTCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

311

Amino Acids

35.5

Weight (kDa)

8.6

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 61 - 164 2.1e-18 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 63 - 300 2.3e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09430
fragaria_vesca FvH4_2g10970
malus_domestica MD05G1044200.v1.1
prunus_persica Prupe.8G059800_v2.0.a1 Prupe.8G059800_v2.0.a1 Prupe.8G059800_v2.0.a1 Prupe.8G059800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0269171
rosa_laevigata RLG00000013875
rosa_multiflora Rmu_ssc0000381.1_g000010
rosa_roxburghii Rroxscaffold_7G00198990
rosa_rugosa Rorug06G0045200 Rorug06G0045200
rosa_samantha Rh6AG166000 Rh6BG171400 Rh6CG165100 Rh6DG156700
rosa_wichuraiana Rw6G014330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 478
AccB1I GGYRCC 2 cut(s) 209, 526
AccI GTMKAC 2 cut(s) 88, 270
AccII CGCG 1 cut(s) 49
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 1 cut(s) 434
AclI AACGTT 1 cut(s) 453
AclWI GGATC 1 cut(s) 749
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 3 cut(s) 552, 874, 893
AcuI CTGAAG 1 cut(s) 16
AcyI GRCGYC 3 cut(s) 44, 210, 569
AfaI GTAC 1 cut(s) 667
AfiI CCNNNNNNNGG 1 cut(s) 620
AflII CTTAAG 1 cut(s) 581
AgsI TTSAA 3 cut(s) 126, 265, 910
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 6 cut(s) 36, 140, 248, 463, 545, 580
AluI AGCT 6 cut(s) 36, 140, 248, 463, 545, 580
Alw26I GTCTC 1 cut(s) 715
AlwI GGATC 1 cut(s) 749
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 2 cut(s) 22, 474
ApoI RAATTY 3 cut(s) 552, 874, 893
AspA2I CCTAGG 1 cut(s) 737
AspLEI GCGC 4 cut(s) 212, 370, 439, 479
AsuHPI GGTGA 2 cut(s) 244, 859
AvrII CCTAGG 1 cut(s) 737
BanI GGYRCC 2 cut(s) 209, 526
BbvI GCAGC 2 cut(s) 9, 461
BceAI ACGGC 2 cut(s) 57, 133
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 338
BclI TGATCA 4 cut(s) 7, 696, 759, 849
BcoDI GTCTC 1 cut(s) 715
BfaI CTAG 3 cut(s) 536, 738, 800
BfoI RGCGCY 1 cut(s) 213
BfrI CTTAAG 1 cut(s) 581
BglII AGATCT 1 cut(s) 106
BisI GCNGC 3 cut(s) 23, 435, 475
BlnI CCTAGG 1 cut(s) 737
BlsI GCNGC 3 cut(s) 24, 436, 476
Bme1390I CCNGG 1 cut(s) 338
BmiI GGNNCC 3 cut(s) 211, 315, 528
BmrFI CCNGG 1 cut(s) 338
BmsI GCATC 2 cut(s) 17, 140
BplI GAGNNNNNCTC 2 cut(s) 225, 257
Bsa29I ATCGAT 2 cut(s) 172, 654
BsaBI GATNNNNATC 1 cut(s) 389
BsaHI GRCGYC 3 cut(s) 44, 210, 569
BsaJI CCNNGG 4 cut(s) 115, 199, 737, 888
BsaWI WCCGGW 2 cut(s) 445, 548
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 1 cut(s) 620
Bse118I RCCGGY 2 cut(s) 83, 254
Bse1I ACTGG 1 cut(s) 798
Bse3DI GCAATG 1 cut(s) 225
Bse8I GATNNNNATC 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 1 cut(s) 338
BseCI ATCGAT 2 cut(s) 172, 654
BseDI CCNNGG 4 cut(s) 115, 199, 737, 888
BseGI GGATG 2 cut(s) 302, 390
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 620
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 1 cut(s) 798
BseRI GAGGAG 6 cut(s) 179, 182, 185, 275, 509, 695
BseX3I CGGCCG 1 cut(s) 256
BseXI GCAGC 2 cut(s) 9, 461
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 1 cut(s) 258
BshNI GGYRCC 2 cut(s) 209, 526
BshVI ATCGAT 2 cut(s) 172, 654
BsiEI CGRYCG 1 cut(s) 259
BsiSI CCGG 6 cut(s) 64, 84, 255, 446, 549, 572
BslI CCNNNNNNNGG 1 cut(s) 620
BsmAI GTCTC 1 cut(s) 715
BsnI GGCC 1 cut(s) 258
Bsp13I TCCGGA 1 cut(s) 445
Bsp68I TCGCGA 1 cut(s) 49
BspACI CCGC 1 cut(s) 434
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 577
BspDI ATCGAT 2 cut(s) 172, 654
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 3 cut(s) 211, 315, 528
BspPI GGATC 1 cut(s) 749
BspT107I GGYRCC 2 cut(s) 209, 526
BspTI CTTAAG 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 225
BsrFI RCCGGY 2 cut(s) 83, 254
BsrI ACTGG 1 cut(s) 798
BssAI RCCGGY 2 cut(s) 83, 254
BssECI CCNNGG 4 cut(s) 115, 199, 737, 888
BssNI GRCGYC 3 cut(s) 44, 210, 569
BssT1I CCWWGG 2 cut(s) 737, 888
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 2 cut(s) 293, 680
BstACI GRCGYC 3 cut(s) 44, 210, 569
BstAFI CTTAAG 1 cut(s) 581
BstAPI GCANNNNNTGC 1 cut(s) 866
BstDEI CTNAG 1 cut(s) 564
BstDSI CCRYGG 2 cut(s) 115, 199
BstF5I GGATG 2 cut(s) 302, 390
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 4 cut(s) 212, 370, 439, 479
BstMAI GTCTC 1 cut(s) 715
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 3 cut(s) 137, 577, 866
BstNI CCWGG 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 869
BstSCI CCNGG 1 cut(s) 336
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 2 cut(s) 9, 461
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BstZI CGGCCG 1 cut(s) 256
Bsu15I ATCGAT 2 cut(s) 172, 654
BsuRI GGCC 1 cut(s) 258
BsuTUI ATCGAT 2 cut(s) 172, 654
BtgI CCRYGG 2 cut(s) 115, 199
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 302, 390
BtsIMutI CAGTG 1 cut(s) 791
BtuMI TCGCGA 1 cut(s) 49
CfoI GCGC 4 cut(s) 212, 370, 439, 479
Cfr10I RCCGGY 2 cut(s) 83, 254
ClaI ATCGAT 2 cut(s) 172, 654
CseI GACGC 2 cut(s) 33, 577
Csp6I GTAC 1 cut(s) 666
CviAII CATG 1 cut(s) 866
CviJI RGCY 8 cut(s) 36, 140, 248, 258, 341, 463, 545, 580
CviKI_1 RGCY 8 cut(s) 36, 140, 248, 258, 341, 463, 545, 580
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 1 cut(s) 564
DinI GGCGCC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 256
EagI CGGCCG 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 293, 680
EarI CTCTTC 2 cut(s) 293, 680
EclXI CGGCCG 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 737, 888
Eco52I CGGCCG 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 737, 888
EcoT22I ATGCAT 1 cut(s) 147
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 737, 888
FaeI CATG 1 cut(s) 869
FalI AAGNNNNNCTT 2 cut(s) 586, 618
FatI CATG 1 cut(s) 865
FauNDI CATATG 2 cut(s) 12, 764
FbaI TGATCA 4 cut(s) 7, 696, 759, 849
FblI GTMKAC 2 cut(s) 88, 270
Fnu4HI GCNGC 3 cut(s) 23, 435, 475
FokI GGATG 2 cut(s) 289, 397
Fsp4HI GCNGC 3 cut(s) 23, 435, 475
FspBI CTAG 3 cut(s) 536, 738, 800
FspI TGCGCA 1 cut(s) 478
GlaI GCGC 4 cut(s) 211, 369, 438, 478
GluI GCNGC 3 cut(s) 23, 435, 475
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 258
HapII CCGG 6 cut(s) 64, 84, 255, 446, 549, 572
HgaI GACGC 2 cut(s) 33, 577
HhaI GCGC 4 cut(s) 212, 370, 439, 479
Hin1I GRCGYC 3 cut(s) 44, 210, 569
Hin1II CATG 1 cut(s) 869
Hin6I GCGC 4 cut(s) 210, 368, 437, 477
HinP1I GCGC 4 cut(s) 210, 368, 437, 477
HindIII AAGCTT 2 cut(s) 461, 578
HpaII CCGG 6 cut(s) 64, 84, 255, 446, 549, 572
HphI GGTGA 2 cut(s) 244, 859
Hpy166II GTNNAC 3 cut(s) 89, 271, 666
Hpy188I TCNGA 5 cut(s) 245, 313, 351, 567, 730
Hpy188III TCNNGA 7 cut(s) 48, 278, 379, 397, 446, 536, 853
Hpy8I GTNNAC 3 cut(s) 89, 271, 666
Hpy99I CGWCG 2 cut(s) 49, 398
HpyAV CCTTC 1 cut(s) 639
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 4 cut(s) 91, 267, 273, 453
HpyCH4V TGCA 2 cut(s) 145, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 577, 866
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 4 cut(s) 91, 267, 273, 453
Hsp92I GRCGYC 3 cut(s) 44, 210, 569
Hsp92II CATG 1 cut(s) 869
HspAI GCGC 4 cut(s) 210, 368, 437, 477
KasI GGCGCC 1 cut(s) 209
Kpn2I TCCGGA 1 cut(s) 445
Ksp22I TGATCA 4 cut(s) 7, 696, 759, 849
Lsp1109I GCAGC 2 cut(s) 9, 461
LweI GCATC 2 cut(s) 17, 140
MaeI CTAG 3 cut(s) 536, 738, 800
MaeII ACGT 4 cut(s) 91, 267, 273, 453
MaeIII GTNAC 3 cut(s) 97, 404, 803
MboII GAAGA 7 cut(s) 280, 283, 551, 697, 725, 785, 788
MflI RGATCY 1 cut(s) 106
MluCI AATT 4 cut(s) 121, 552, 874, 893
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 2 cut(s) 384, 920
MmeI TCCRAC 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 147
MroI TCCGGA 1 cut(s) 445
MseI TTAA 5 cut(s) 359, 522, 582, 609, 812
MspCI CTTAAG 1 cut(s) 581
MspI CCGG 6 cut(s) 64, 84, 255, 446, 549, 572
MspR9I CCNGG 1 cut(s) 338
MvaI CCWGG 1 cut(s) 338
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 137, 577, 866
NarI GGCGCC 1 cut(s) 210
NdeI CATATG 2 cut(s) 12, 764
NlaIII CATG 1 cut(s) 869
NlaIV GGNNCC 3 cut(s) 211, 315, 528
NruI TCGCGA 1 cut(s) 49
NsbI TGCGCA 1 cut(s) 478
NsiI ATGCAT 1 cut(s) 147
NspI RCATGY 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 9 cut(s) 154, 169, 204, 261, 329, 414, 532, 559, 597
PkrI GCNGC 3 cut(s) 24, 436, 476
PleI GAGTC 2 cut(s) 383, 920
PluTI GGCGCC 1 cut(s) 213
PpsI GAGTC 2 cut(s) 383, 920
Psp1406I AACGTT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 3 cut(s) 211, 315, 528
PsuI RGATCY 1 cut(s) 106
RruI TCGCGA 1 cut(s) 49
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
SaqAI TTAA 5 cut(s) 359, 522, 582, 609, 812
SatI GCNGC 3 cut(s) 23, 435, 475
SchI GAGTC 2 cut(s) 384, 920
ScrFI CCNGG 1 cut(s) 338
SfaNI GCATC 2 cut(s) 17, 140
SfoI GGCGCC 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 4 cut(s) 121, 552, 874, 893
SsiI CCGC 1 cut(s) 434
SspDI GGCGCC 1 cut(s) 209
SspI AATATT 1 cut(s) 786
SspMI CTAG 3 cut(s) 536, 738, 800
StyD4I CCNGG 1 cut(s) 336
StyI CCWWGG 2 cut(s) 737, 888
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 4 cut(s) 94, 270, 276, 456
TaqI TCGA 2 cut(s) 172, 654
TasI AATT 4 cut(s) 121, 552, 874, 893
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 9 cut(s) 154, 169, 204, 261, 329, 414, 532, 559, 597
Tru1I TTAA 5 cut(s) 359, 522, 582, 609, 812
Tru9I TTAA 5 cut(s) 359, 522, 582, 609, 812
TscAI CASTG 1 cut(s) 798
TseI GCWGC 2 cut(s) 22, 474
TspDTI ATGAA 2 cut(s) 29, 401
TspGWI ACGGA 6 cut(s) 85, 216, 246, 387, 528, 683
TspRI CASTG 1 cut(s) 798
Vha464I CTTAAG 1 cut(s) 581
XapI RAATTY 3 cut(s) 552, 874, 893
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 1 cut(s) 869
XmaJI CCTAGG 1 cut(s) 737
XmiI GTMKAC 2 cut(s) 88, 270
XspI CTAG 3 cut(s) 536, 738, 800
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.