Rh6CG165100

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013156
Physical Location & Seq
Forward (+)
22395252 .. 22408106
12855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG165100.1

Sequence Viewer

Length: 1092 bp
ATGTTTCATCATCTCAAGTTTCCCAAATGCTTCAGTTTGACGGCTTCAATAGATTGGTTTTACCGCCATTATTTTGTCAATGCTGGTCTTCGGTCTGTGATAACTGATCTAGGTGATGGTGATGACACCAACATGCATTGTTGGGTGCCTAAGATTCATAAACCATTCAAACCCAACATCATCTTGATTCATGGGTTCGGAGCCAATGCAATGTGGCAGTACGGTGAGCACATTCGTCATTTCACCTCTCATTTTAACGTCTACGTGCCCGATCTTGTTTTCTTTGGAAACTCTTACACGTCCAGCCAAGAACGAACTGATTTGTTCCAGGCTACATGTGTAATTAAGTTGATGCAGGCCCATAGGGTTGAAAAGACAAGCGTTGTTGGGATAAGTTATGGTGGATTTGTGGGGTATAGTATAGCGGTGCAATGTCCGGAGATGGTAGAAAAGTTGGTATTGTGTTGTGCTGGAGTTTGCATGGAGGAGAAAGATATGGAAAATGGACTGTTTAATGTTTCTAATTTAGAGGAGGCTTCAAGTATTTTGTTACCACAAACACCTCAAAAATTGAAGGAACTAATGCGATATTCATTCTTCAAGCCTGCTAAGGCCGTCCCTTCTTTTTTCCTCACCGATTTTATTAATGTAATGTGTACGAACTATGTTGATGAGAAGAGAGAGTTGATTGGGGCAATATTAGCGAACCGAAAACTGTCTAATTTACCCAAGATCACACAGCCCACATTAATAATATGGGGAGAGCAAGATCAAATATTCCCTTTGGAACTAGGTTACAGATTAAAAAGGCATATTGGGAAACACGGTCAACTAGTGATTATTAAAAAGGCTGGACATGCTGTGAACCTGGAACAACACAAGGAGTTTATAAAGCATTTAAAATCGTTTCTTTGTGACTTATTTTGGTCTCCTCCACCATCACCAACATGGAAAGATCACTTTCATGATCACATCAACAAAACCAAGTTGGGGCTTAGCTTCTACGTCGCCGACCCCTGCAACACCGCTCAGTCACCCACCGACCTCCTCCAAAAGGCAGAGACCGTCATTCCAAAGATCTTCGATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

363

Amino Acids

41.55

Weight (kDa)

8.24

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 58 - 291 1.1e-20 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 60 - 297 1.5e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09430
fragaria_vesca FvH4_2g10970
malus_domestica MD05G1044200.v1.1
prunus_persica Prupe.8G059800_v2.0.a1 Prupe.8G059800_v2.0.a1 Prupe.8G059800_v2.0.a1 Prupe.8G059800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0269171
rosa_laevigata RLG00000013875
rosa_multiflora Rmu_ssc0000381.1_g000010
rosa_roxburghii Rroxscaffold_7G00198990
rosa_rugosa Rorug06G0045200 Rorug06G0045200
rosa_samantha Rh6AG166000 Rh6BG171400 Rh6CG165100 Rh6DG156700
rosa_wichuraiana Rw6G014330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
AccB1I GGYRCC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 3 cut(s) 64, 425, 1026
AclWI GGATC 1 cut(s) 1079
AcuI CTGAAG 1 cut(s) 16
AfaI GTAC 2 cut(s) 221, 658
AfiI CCNNNNNNNGG 2 cut(s) 990, 1054
AflIII ACRYGT 2 cut(s) 297, 335
AgsI TTSAA 6 cut(s) 48, 169, 371, 540, 574, 601
AhlI ACTAGT 1 cut(s) 832
AjiI CACGTC 1 cut(s) 300
AjnI CCWGG 2 cut(s) 327, 867
AluBI AGCT 1 cut(s) 999
AluI AGCT 1 cut(s) 999
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 933, 1055
AlwI GGATC 1 cut(s) 1079
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 2 cut(s) 357, 612
AseI ATTAAT 2 cut(s) 645, 749
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 7 cut(s) 125, 131, 235, 236, 625, 933, 1026
BaeGI GKGCMC 1 cut(s) 270
BanI GGYRCC 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 3 cut(s) 110, 436, 946
BceAI ACGGC 2 cut(s) 57, 599
BciT130I CCWGG 2 cut(s) 329, 869
BclI TGATCA 1 cut(s) 967
BcoDI GTCTC 2 cut(s) 933, 1055
BcuI ACTAGT 1 cut(s) 832
BfaI CTAG 3 cut(s) 110, 791, 833
BglII AGATCT 1 cut(s) 1077
BlpI GCTNAGC 1 cut(s) 995
Bme1390I CCNGG 2 cut(s) 329, 869
BmgBI CACGTC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 147, 202
BmrFI CCNGG 2 cut(s) 329, 869
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 609
Bpu1102I GCTNAGC 1 cut(s) 995
BsaAI YACGTR 1 cut(s) 265
BsaI GGTCTC 2 cut(s) 933, 1055
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 2 cut(s) 990, 1054
Bse3DI GCAATG 2 cut(s) 216, 437
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 2 cut(s) 329, 869
BseLI CCNNNNNNNGG 2 cut(s) 990, 1054
BseMI GCAATG 2 cut(s) 216, 437
BseMII CTCAG 1 cut(s) 1043
BseRI GAGGAG 4 cut(s) 500, 545, 921, 1037
BseSI GKGCMC 1 cut(s) 270
BshFI GGCC 2 cut(s) 359, 614
BshNI GGYRCC 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 990, 1054
BsmAI GTCTC 2 cut(s) 933, 1055
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 2 cut(s) 359, 614
Bso31I GGTCTC 2 cut(s) 933, 1055
Bsp1286I GDGCHC 2 cut(s) 231, 270
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
Bsp1720I GCTNAGC 1 cut(s) 995
BspACI CCGC 3 cut(s) 64, 425, 1026
BspANI GGCC 2 cut(s) 359, 614
BspCNI CTCAG 1 cut(s) 1042
BspEI TCCGGA 1 cut(s) 436
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 2 cut(s) 147, 202
BspPI GGATC 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 145
BspTNI GGTCTC 2 cut(s) 933, 1055
BsrBI CCGCTC 1 cut(s) 1028
BsrDI GCAATG 2 cut(s) 216, 437
BssMI GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
Bst2UI CCWGG 2 cut(s) 329, 869
Bst4CI ACNGT 5 cut(s) 224, 510, 717, 827, 1066
Bst6I CTCTTC 1 cut(s) 671
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 2 cut(s) 357, 606
BstDEI CTNAG 4 cut(s) 150, 609, 995, 1029
BstENI CCTNNNNNAGG 1 cut(s) 1052
BstKTI GATC 8 cut(s) 109, 274, 735, 772, 958, 970, 1080, 1087
BstMAI GTCTC 2 cut(s) 933, 1055
BstMBI GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
BstMWI GCNNNNNNNGC 2 cut(s) 701, 857
BstNI CCWGG 2 cut(s) 329, 869
BstNSI RCATGY 3 cut(s) 136, 339, 860
BstSCI CCNGG 2 cut(s) 327, 867
BstSLI GKGCMC 1 cut(s) 270
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 1077
BstYI RGATCY 1 cut(s) 1077
BsuRI GGCC 2 cut(s) 359, 614
BtrI CACGTC 1 cut(s) 300
Cac8I GCNNGC 2 cut(s) 357, 606
CciI TCATGA 1 cut(s) 964
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 2 cut(s) 220, 657
CviAII CATG 8 cut(s) 133, 191, 336, 481, 857, 948, 965, 1089
CviQI GTAC 2 cut(s) 220, 657
DdeI CTNAG 4 cut(s) 150, 609, 995, 1029
DpnI GATC 8 cut(s) 108, 273, 734, 771, 957, 969, 1079, 1086
DpnII GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
DraI TTTAAA 1 cut(s) 900
Eam1104I CTCTTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 671
Eco31I GGTCTC 2 cut(s) 933, 1055
Eco57I CTGAAG 1 cut(s) 16
EcoNI CCTNNNNNAGG 1 cut(s) 1052
EcoRII CCWGG 2 cut(s) 327, 867
EcoT22I ATGCAT 1 cut(s) 138
FaeI CATG 8 cut(s) 136, 194, 339, 484, 860, 951, 968, 1092
FaqI GGGAC 1 cut(s) 602
FatI CATG 8 cut(s) 132, 190, 335, 480, 856, 947, 964, 1088
FbaI TGATCA 1 cut(s) 967
FblI GTMKAC 1 cut(s) 261
FspBI CTAG 3 cut(s) 110, 791, 833
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 2 cut(s) 359, 614
HapII CCGG 1 cut(s) 437
Hin1II CATG 8 cut(s) 136, 194, 339, 484, 860, 951, 968, 1092
HincII GTYRAC 1 cut(s) 830
HindII GTYRAC 1 cut(s) 830
HinfI GANTC 2 cut(s) 154, 187
HpaII CCGG 1 cut(s) 437
HphI GGTGA 7 cut(s) 125, 131, 235, 236, 625, 933, 1026
Hpy166II GTNNAC 4 cut(s) 262, 657, 830, 865
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 3 cut(s) 184, 437, 965
Hpy8I GTNNAC 4 cut(s) 262, 657, 830, 865
Hpy99I CGWCG 1 cut(s) 1010
HpyAV CCTTC 2 cut(s) 568, 630
HpyCH4III ACNGT 5 cut(s) 224, 510, 717, 827, 1066
HpyCH4IV ACGT 4 cut(s) 258, 264, 299, 1005
HpyCH4V TGCA 6 cut(s) 136, 209, 355, 430, 480, 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 701, 857
HpyF3I CTNAG 4 cut(s) 150, 609, 995, 1029
HpySE526I ACGT 4 cut(s) 258, 264, 299, 1005
Hsp92II CATG 8 cut(s) 136, 194, 339, 484, 860, 951, 968, 1092
Kpn2I TCCGGA 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
LmnI GCTCC 1 cut(s) 200
LweI GCATC 1 cut(s) 342
MaeI CTAG 3 cut(s) 110, 791, 833
MaeII ACGT 4 cut(s) 258, 264, 299, 1005
MaeIII GTNAC 4 cut(s) 549, 794, 914, 1032
MalI GATC 8 cut(s) 108, 273, 734, 771, 957, 969, 1079, 1086
MbiI CCGCTC 1 cut(s) 1028
MboI GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
MboII GAAGA 4 cut(s) 80, 589, 688, 1072
MflI RGATCY 1 cut(s) 1077
MhlI GDGCHC 2 cut(s) 231, 270
MluCI AATT 4 cut(s) 342, 523, 569, 721
MnlI CCTC 9 cut(s) 256, 478, 523, 526, 573, 641, 942, 1055, 1058
Mph1103I ATGCAT 1 cut(s) 138
MroI TCCGGA 1 cut(s) 436
MseI TTAA 8 cut(s) 255, 345, 513, 645, 749, 803, 843, 899
MslI CAYNNNNRTG 3 cut(s) 131, 946, 963
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 329, 869
MvaI CCWGG 2 cut(s) 329, 869
MwoI GCNNNNNNNGC 2 cut(s) 701, 857
NdeII GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
NlaIII CATG 8 cut(s) 136, 194, 339, 484, 860, 951, 968, 1092
NlaIV GGNNCC 2 cut(s) 147, 202
NmuCI GTSAC 2 cut(s) 914, 1032
NsiI ATGCAT 1 cut(s) 138
NspI RCATGY 3 cut(s) 136, 339, 860
PagI TCATGA 1 cut(s) 964
PciI ACATGT 1 cut(s) 335
PfeI GAWTC 2 cut(s) 154, 187
Ppu21I YACGTR 1 cut(s) 265
PscI ACATGT 1 cut(s) 335
PshBI ATTAAT 2 cut(s) 645, 749
PsiI TTATAA 1 cut(s) 890
Psp6I CCWGG 2 cut(s) 327, 867
PspGI CCWGG 2 cut(s) 327, 867
PspN4I GGNNCC 2 cut(s) 147, 202
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 1077
RsaI GTAC 2 cut(s) 221, 658
RsaNI GTAC 2 cut(s) 220, 657
RseI CAYNNNNRTG 3 cut(s) 131, 946, 963
SaqAI TTAA 8 cut(s) 255, 345, 513, 645, 749, 803, 843, 899
Sau3AI GATC 8 cut(s) 106, 271, 732, 769, 955, 967, 1077, 1084
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 2 cut(s) 329, 869
SduI GDGCHC 2 cut(s) 231, 270
SfaNI GCATC 1 cut(s) 342
SmiMI CAYNNNNRTG 3 cut(s) 131, 946, 963
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
SpeI ACTAGT 1 cut(s) 832
Sse9I AATT 4 cut(s) 342, 523, 569, 721
SsiI CCGC 3 cut(s) 64, 425, 1026
SspI AATATT 2 cut(s) 699, 777
SspMI CTAG 3 cut(s) 110, 791, 833
StyD4I CCNGG 2 cut(s) 327, 867
TaaI ACNGT 5 cut(s) 224, 510, 717, 827, 1066
TaiI ACGT 4 cut(s) 261, 267, 302, 1008
TaqI TCGA 1 cut(s) 1083
TaqII GACCGA 1 cut(s) 81
TasI AATT 4 cut(s) 342, 523, 569, 721
TfiI GAWTC 2 cut(s) 154, 187
Tru1I TTAA 8 cut(s) 255, 345, 513, 645, 749, 803, 843, 899
Tru9I TTAA 8 cut(s) 255, 345, 513, 645, 749, 803, 843, 899
TseFI GTSAC 2 cut(s) 914, 1032
Tsp45I GTSAC 2 cut(s) 914, 1032
TspDTI ATGAA 4 cut(s) 146, 179, 582, 953
VspI ATTAAT 2 cut(s) 645, 749
XagI CCTNNNNNAGG 1 cut(s) 1052
XceI RCATGY 3 cut(s) 136, 339, 860
XcmI CCANNNNNNNNNTGG 1 cut(s) 945
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 3 cut(s) 110, 791, 833
Zsp2I ATGCAT 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.